MD15G1076800.v1.1

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003321
Physical Location & Seq
Forward (+)
5231664 .. 5232684
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1076800.v1.1.491

Sequence Viewer

Length: 510 bp
ATGACATTTGCCAATGAGGTCCTCCGAGCCAAGGGGTCGGGATTTGGGGAAGTGGGTCTGGTTCCTTGCGCCGTAGGCGGGACTAGAATAGGCGAGTGGGGTAAAGTGACTCGGTTGTATAGTGAGTTGGTGAGACGAGCCCGCGAGTCGGTCAGGGGCGGTGGAGTTATCCGGGCGCTTTTGTGGTTTCAGGGAGAGAGTGACACCGTGAGAAAGGAGGATGCTGAGGGGTATAAAGGGAATTTCGAGATGCTTGTTTTGGACCTGCGTTCTGGTCTTCATCTCCCTAACCTTCCTGTCATCCAGGTGGCACTGGCTTCTGGAGAAGGCAAATTTGTAGAAATAGTGAGGAAAGGTCAGTTGGATGTCAAGTTACCAAATGTGAAATGCGTTGATGCCAAAGGATTGCGTCTCAAAGAAGATGGCCTCCACCTTACTACTGTATCTGAAGTCCACTTGGGCATCAAGCTTGCTCGTTCCTTCCTTGATTCTTATGGCCACCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

170

Amino Acids

18.48

Weight (kDa)

9.17

Isoelectric Point (pI)

22.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 1 - 162 2.4e-55 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 273
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 78, 142, 159
AcoI YGGCCR 1 cut(s) 496
AcsI RAATTY 2 cut(s) 241, 332
AcuI CTGAAG 1 cut(s) 468
AfiI CCNNNNNNNGG 3 cut(s) 31, 78, 148
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 1 cut(s) 469
AluI AGCT 1 cut(s) 469
Alw26I GTCTC 2 cut(s) 127, 416
AoxI GGCC 2 cut(s) 424, 496
ApoI RAATTY 2 cut(s) 241, 332
AspLEI GCGC 2 cut(s) 71, 178
AspS9I GGNCC 2 cut(s) 19, 262
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 2 cut(s) 19, 262
BaeI ACNNNNGTAYC 2 cut(s) 426, 459
BalI TGGCCA 1 cut(s) 498
BanII GRGCYC 1 cut(s) 142
BbsI GAAGAC 1 cut(s) 269
BbvCI CCTCAGC 1 cut(s) 225
BccI CCATC 1 cut(s) 416
BceAI ACGGC 1 cut(s) 56
BciT130I CCWGG 1 cut(s) 305
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 127, 416
BfaI CTAG 1 cut(s) 84
BfoI RGCGCY 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 273
Bme1390I CCNGG 2 cut(s) 173, 305
Bme18I GGWCC 2 cut(s) 19, 262
BmgT120I GGNCC 2 cut(s) 19, 262
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 2 cut(s) 173, 305
BmsI GCATC 4 cut(s) 211, 240, 385, 471
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 30
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 78, 148
Bse1I ACTGG 1 cut(s) 318
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 3 cut(s) 226, 300, 370
BseLI CCNNNNNNNGG 3 cut(s) 31, 78, 148
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 426, 498
BsiSI CCGG 1 cut(s) 172
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 3 cut(s) 31, 78, 148
BsmAI GTCTC 2 cut(s) 127, 416
BsmBI CGTCTC 2 cut(s) 127, 416
BsmFI GGGAC 1 cut(s) 94
BsnI GGCC 2 cut(s) 426, 498
Bsp1286I GDGCHC 1 cut(s) 142
BspACI CCGC 3 cut(s) 78, 142, 159
BspANI GGCC 2 cut(s) 426, 498
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 63
BspMI ACCTGC 1 cut(s) 273
BsrI ACTGG 1 cut(s) 318
BssECI CCNNGG 1 cut(s) 30
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 208, 442
BstC8I GCNNGC 2 cut(s) 142, 471
BstDEI CTNAG 1 cut(s) 225
BstF5I GGATG 3 cut(s) 226, 300, 370
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 2 cut(s) 71, 178
BstMAI GTCTC 2 cut(s) 127, 416
BstMWI GCNNNNNNNGC 1 cut(s) 75
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 171, 303
BstUI CGCG 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 269
BsuRI GGCC 2 cut(s) 426, 498
BtsCI GGATG 3 cut(s) 226, 300, 370
BtsIMutI CAGTG 1 cut(s) 311
BveI ACCTGC 1 cut(s) 273
Cac8I GCNNGC 2 cut(s) 142, 471
CfoI GCGC 2 cut(s) 71, 178
Cfr13I GGNCC 2 cut(s) 19, 262
CseI GACGC 1 cut(s) 398
CspCI CAANNNNNGTGG 1 cut(s) 488
CviJI RGCY 6 cut(s) 29, 140, 317, 426, 469, 498
CviKI_1 RGCY 6 cut(s) 29, 140, 317, 426, 469, 498
DdeI CTNAG 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 496
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 19, 262
Eco57I CTGAAG 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 30
EcoT38I GRGCYC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 30
Esp3I CGTCTC 2 cut(s) 127, 416
FaiI YATR 3 cut(s) 120, 234, 495
FaqI GGGAC 1 cut(s) 94
FauI CCCGC 2 cut(s) 71, 149
FokI GGATG 3 cut(s) 233, 287, 377
FriOI GRGCYC 1 cut(s) 142
FspBI CTAG 1 cut(s) 84
GlaI GCGC 2 cut(s) 70, 177
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 2 cut(s) 426, 498
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 398
HhaI GCGC 2 cut(s) 71, 178
Hin6I GCGC 2 cut(s) 69, 176
HinP1I GCGC 2 cut(s) 69, 176
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 3 cut(s) 109, 146, 488
HpaII CCGG 1 cut(s) 172
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 2 cut(s) 26, 448
Hpy188III TCNNGA 3 cut(s) 39, 247, 321
Hpy8I GTNNAC 1 cut(s) 454
HpyAV CCTTC 3 cut(s) 302, 320, 490
HpyCH4III ACNGT 2 cut(s) 208, 442
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
HpyF3I CTNAG 1 cut(s) 225
HspAI GCGC 2 cut(s) 69, 176
LweI GCATC 4 cut(s) 211, 240, 385, 471
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 106, 200, 372
MboII GAAGA 2 cut(s) 269, 431
MhlI GDGCHC 1 cut(s) 142
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 2 cut(s) 241, 332
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 2 cut(s) 103, 155
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 6 cut(s) 10, 32, 211, 220, 342, 437
Mox20I TGGCCA 1 cut(s) 498
MscI TGGCCA 1 cut(s) 498
MslI CAYNNNNRTG 1 cut(s) 305
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 2 cut(s) 173, 305
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 75
NciI CCSGG 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 2 cut(s) 106, 200
PfeI GAWTC 1 cut(s) 488
PleI GAGTC 2 cut(s) 103, 154
PpsI GAGTC 2 cut(s) 103, 154
PpuMI RGGWCCY 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 19, 262
PspPPI RGGWCCY 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 305
Sau96I GGNCC 2 cut(s) 19, 262
SchI GAGTC 2 cut(s) 103, 155
ScrFI CCNGG 2 cut(s) 173, 305
SduI GDGCHC 1 cut(s) 142
SetI ASST 7 cut(s) 21, 267, 294, 309, 358, 435, 471
SfaNI GCATC 4 cut(s) 211, 240, 385, 471
SinI GGWCC 2 cut(s) 19, 262
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 2 cut(s) 241, 332
SsiI CCGC 3 cut(s) 78, 142, 159
SspMI CTAG 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 171, 303
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 2 cut(s) 208, 442
TaqI TCGA 1 cut(s) 246
TaqII GACCGA 1 cut(s) 139
TasI AATT 2 cut(s) 241, 332
TfiI GAWTC 1 cut(s) 488
TscAI CASTG 1 cut(s) 318
TseFI GTSAC 2 cut(s) 106, 200
Tsp45I GTSAC 2 cut(s) 106, 200
TspDTI ATGAA 1 cut(s) 269
TspRI CASTG 1 cut(s) 318
VpaK11BI GGWCC 2 cut(s) 19, 262
XapI RAATTY 2 cut(s) 241, 332
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.