Rh6BG069000

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003321
Physical Location & Seq
Forward (+)
10551212 .. 10553580
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG069000.1

Sequence Viewer

Length: 846 bp
ATGGGTTTGGGTTTCTGTTCTGATTCTTCTGACGATGAATTTATGCTCTTGTGCACTGTTTCACTACTATTAATAGCCGGAGAACAACACCGACTCGCTAACCGGAAGCGGCGAAGAACTCGCCGTCGCGGCACAACTACACGAAGAAGACGAAACGACGGCGTTCCGCGCCAAGCCTACGCTGGGCCCCACTCGCCTGCTTTGACAAGCGACGCGGAGAGCCCATTTAAACCAGAGAACTCGCTCATTCCAAGAACAAAACCCGACACGTCGTTTATCCTCCACCCGAAAAACCCGGAAATGGAGTCGCCGGAGCAGATCTTCTTTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGTCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACTCCAAGAAGGTGTGCGGTTTGGGTCCCGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATTATCCAGCTCTACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

281

Amino Acids

31.35

Weight (kDa)

8.67

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 105 - 278 1.2e-70 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 714
AccB1I GGYRCC 2 cut(s) 589, 714
AccII CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
AclWI GGATC 2 cut(s) 361, 436
AcoI YGGCCR 1 cut(s) 379
AcsI RAATTY 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 408
AfaI GTAC 3 cut(s) 647, 716, 761
AfiI CCNNNNNNNGG 5 cut(s) 183, 301, 400, 469, 472
AflIII ACRYGT 2 cut(s) 267, 732
AhdI GACNNNNNGTC 1 cut(s) 736
AjiI CACGTC 2 cut(s) 270, 735
AjnI CCWGG 1 cut(s) 717
AluBI AGCT 1 cut(s) 835
AluI AGCT 1 cut(s) 835
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 744
Alw44I GTGCAC 1 cut(s) 52
AlwI GGATC 2 cut(s) 361, 436
AoxI GGCC 2 cut(s) 185, 379
ApaI GGGCCC 1 cut(s) 189
ApaLI GTGCAC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 38
AseI ATTAAT 1 cut(s) 71
Asp718I GGTACC 1 cut(s) 714
AspLEI GCGC 7 cut(s) 171, 481, 567, 599, 631, 670, 798
AspS9I GGNCC 3 cut(s) 185, 186, 530
AsuC2I CCSGG 2 cut(s) 296, 534
AsuHPI GGTGA 3 cut(s) 452, 568, 670
AvaII GGWCC 1 cut(s) 530
BaeGI GKGCMC 2 cut(s) 56, 189
BanI GGYRCC 2 cut(s) 589, 714
BanII GRGCYC 2 cut(s) 189, 224
BauI CACGAG 1 cut(s) 764
BbsI GAAGAC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 56
BceAI ACGGC 6 cut(s) 108, 175, 394, 421, 577, 627
BciT130I CCWGG 1 cut(s) 719
BcnI CCSGG 2 cut(s) 296, 534
BcoDI GTCTC 1 cut(s) 744
BglI GCCNNNNNGGC 1 cut(s) 129
BglII AGATCT 1 cut(s) 318
BisI GCNGC 5 cut(s) 110, 130, 361, 477, 607
BlsI GCNGC 5 cut(s) 111, 131, 362, 478, 608
Bme1390I CCNGG 3 cut(s) 296, 534, 719
Bme18I GGWCC 1 cut(s) 530
BmeRI GACNNNNNGTC 1 cut(s) 736
BmgBI CACGTC 2 cut(s) 270, 735
BmgT120I GGNCC 3 cut(s) 185, 186, 530
BmiI GGNNCC 6 cut(s) 187, 188, 531, 532, 591, 716
BmrFI CCNGG 3 cut(s) 296, 534, 719
BmrI ACTGGG 2 cut(s) 409, 478
BmsI GCATC 2 cut(s) 649, 745
BmuI ACTGGG 2 cut(s) 409, 478
BpiI GAAGAC 1 cut(s) 154
BpuMI CCSGG 2 cut(s) 296, 534
BsaHI GRCGYC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 376, 718
BsaWI WCCGGW 1 cut(s) 102
BsaXI ACNNNNNCTCC 6 cut(s) 413, 443, 491, 521, 629, 659
Bsc4I CCNNNNNNNGG 5 cut(s) 183, 301, 400, 469, 472
Bse1I ACTGG 2 cut(s) 404, 473
BseBI CCWGG 1 cut(s) 719
BseDI CCNNGG 2 cut(s) 376, 718
BseLI CCNNNNNNNGG 5 cut(s) 183, 301, 400, 469, 472
BseMII CTCAG 2 cut(s) 440, 755
BseNI ACTGG 2 cut(s) 404, 473
BseSI GKGCMC 2 cut(s) 56, 189
BseYI CCCAGC 1 cut(s) 182
Bsh1236I CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
BshFI GGCC 2 cut(s) 187, 381
BshNI GGYRCC 2 cut(s) 589, 714
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 5 cut(s) 78, 103, 296, 311, 534
BslFI GGGAC 2 cut(s) 416, 516
BslI CCNNNNNNNGG 5 cut(s) 183, 301, 400, 469, 472
BsmAI GTCTC 1 cut(s) 744
BsmBI CGTCTC 1 cut(s) 744
BsmFI GGGAC 2 cut(s) 416, 516
BsnI GGCC 2 cut(s) 187, 381
Bsp120I GGGCCC 1 cut(s) 185
Bsp1286I GDGCHC 3 cut(s) 56, 189, 224
Bsp143I GATC 5 cut(s) 318, 366, 441, 615, 696
BspANI GGCC 2 cut(s) 187, 381
BspCNI CTCAG 2 cut(s) 439, 754
BspFNI CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
BspLI GGNNCC 6 cut(s) 187, 188, 531, 532, 591, 716
BspPI GGATC 2 cut(s) 361, 436
BspT107I GGYRCC 2 cut(s) 589, 714
BsrI ACTGG 2 cut(s) 404, 473
BssECI CCNNGG 2 cut(s) 376, 718
BssMI GATC 5 cut(s) 318, 366, 441, 615, 696
BssNI GRCGYC 1 cut(s) 408
BssSI CACGAG 1 cut(s) 764
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 1 cut(s) 719
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 1 cut(s) 656
BstACI GRCGYC 1 cut(s) 408
BstC8I GCNNGC 1 cut(s) 198
BstDEI CTNAG 2 cut(s) 426, 741
BstDSI CCRYGG 1 cut(s) 376
BstFNI CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
BstHHI GCGC 7 cut(s) 171, 481, 567, 599, 631, 670, 798
BstKTI GATC 5 cut(s) 321, 369, 444, 618, 699
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 5 cut(s) 318, 366, 441, 615, 696
BstMWI GCNNNNNNNGC 5 cut(s) 129, 168, 193, 603, 637
BstNI CCWGG 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 294, 532, 717
BstSLI GKGCMC 2 cut(s) 56, 189
BstUI CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
BstV2I GAAGAC 1 cut(s) 154
BstX2I RGATCY 1 cut(s) 318
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 2 cut(s) 187, 381
BtgI CCRYGG 1 cut(s) 376
BtrI CACGTC 2 cut(s) 270, 735
BtsIMutI CAGTG 3 cut(s) 54, 397, 466
Cac8I GCNNGC 1 cut(s) 198
CfoI GCGC 7 cut(s) 171, 481, 567, 599, 631, 670, 798
Cfr13I GGNCC 3 cut(s) 185, 186, 530
CseI GACGC 2 cut(s) 221, 397
Csp6I GTAC 3 cut(s) 646, 715, 760
CviAII CATG 5 cut(s) 346, 655, 746, 772, 800
CviQI GTAC 3 cut(s) 646, 715, 760
DdeI CTNAG 2 cut(s) 426, 741
DpnI GATC 5 cut(s) 320, 368, 443, 617, 698
DpnII GATC 5 cut(s) 318, 366, 441, 615, 696
DraI TTTAAA 1 cut(s) 229
DriI GACNNNNNGTC 1 cut(s) 736
EaeI YGGCCR 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 656
Eam1105I GACNNNNNGTC 1 cut(s) 736
EarI CTCTTC 1 cut(s) 656
EciI GGCGGA 1 cut(s) 437
Eco24I GRGCYC 2 cut(s) 189, 224
Eco47I GGWCC 1 cut(s) 530
EcoO109I RGGNCCY 2 cut(s) 186, 530
EcoRII CCWGG 1 cut(s) 717
EcoT38I GRGCYC 2 cut(s) 189, 224
Esp3I CGTCTC 1 cut(s) 744
FaeI CATG 5 cut(s) 349, 658, 749, 775, 803
FaiI YATR 6 cut(s) 44, 347, 656, 747, 773, 801
FaqI GGGAC 2 cut(s) 416, 516
FatI CATG 5 cut(s) 345, 654, 745, 771, 799
FauI CCCGC 1 cut(s) 343
Fnu4HI GCNGC 5 cut(s) 110, 130, 361, 477, 607
FriOI GRGCYC 2 cut(s) 189, 224
Fsp4HI GCNGC 5 cut(s) 110, 130, 361, 477, 607
GlaI GCGC 7 cut(s) 170, 480, 566, 598, 630, 669, 797
GluI GCNGC 5 cut(s) 110, 130, 361, 477, 607
GsaI CCCAGC 1 cut(s) 186
HaeIII GGCC 2 cut(s) 187, 381
HapII CCGG 5 cut(s) 78, 103, 296, 311, 534
HgaI GACGC 2 cut(s) 221, 397
HhaI GCGC 7 cut(s) 171, 481, 567, 599, 631, 670, 798
Hin1I GRCGYC 1 cut(s) 408
Hin1II CATG 5 cut(s) 349, 658, 749, 775, 803
Hin6I GCGC 7 cut(s) 169, 479, 565, 597, 629, 668, 796
HinP1I GCGC 7 cut(s) 169, 479, 565, 597, 629, 668, 796
HinfI GANTC 4 cut(s) 23, 93, 305, 506
HpaII CCGG 5 cut(s) 78, 103, 296, 311, 534
HphI GGTGA 3 cut(s) 452, 568, 670
Hpy166II GTNNAC 1 cut(s) 54
Hpy188I TCNGA 4 cut(s) 22, 31, 421, 433
Hpy8I GTNNAC 1 cut(s) 54
Hpy99I CGWCG 7 cut(s) 129, 161, 215, 274, 413, 421, 739
HpyAV CCTTC 3 cut(s) 447, 508, 825
HpyCH4III ACNGT 1 cut(s) 58
HpyCH4IV ACGT 2 cut(s) 269, 734
HpyCH4V TGCA 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 5 cut(s) 129, 168, 193, 603, 637
HpyF3I CTNAG 2 cut(s) 426, 741
HpySE526I ACGT 2 cut(s) 269, 734
Hsp92I GRCGYC 1 cut(s) 408
Hsp92II CATG 5 cut(s) 349, 658, 749, 775, 803
HspAI GCGC 7 cut(s) 169, 479, 565, 597, 629, 668, 796
KflI GGGWCCC 1 cut(s) 530
KpnI GGTACC 1 cut(s) 718
Kzo9I GATC 5 cut(s) 318, 366, 441, 615, 696
LmnI GCTCC 2 cut(s) 313, 637
LweI GCATC 2 cut(s) 649, 745
MaeII ACGT 2 cut(s) 269, 734
MaeIII GTNAC 1 cut(s) 371
MalI GATC 5 cut(s) 320, 368, 443, 617, 698
MboI GATC 5 cut(s) 318, 366, 441, 615, 696
MboII GAAGA 8 cut(s) 18, 126, 156, 159, 313, 589, 673, 694
MflI RGATCY 1 cut(s) 318
MhlI GDGCHC 3 cut(s) 56, 189, 224
MluCI AATT 2 cut(s) 38, 825
MlyI GAGTC 3 cut(s) 87, 314, 500
MmeI TCCRAC 2 cut(s) 399, 456
MseI TTAA 2 cut(s) 71, 228
MspI CCGG 5 cut(s) 78, 103, 296, 311, 534
MspR9I CCNGG 3 cut(s) 296, 534, 719
MvaI CCWGG 1 cut(s) 719
MvnI CGCG 9 cut(s) 129, 169, 215, 481, 554, 567, 599, 631, 670
MwoI GCNNNNNNNGC 5 cut(s) 129, 168, 193, 603, 637
NciI CCSGG 2 cut(s) 296, 534
NdeII GATC 5 cut(s) 318, 366, 441, 615, 696
NlaIII CATG 5 cut(s) 349, 658, 749, 775, 803
NlaIV GGNNCC 6 cut(s) 187, 188, 531, 532, 591, 716
NmuCI GTSAC 1 cut(s) 371
PcsI WCGNNNNNNNCGW 2 cut(s) 148, 501
PfeI GAWTC 1 cut(s) 23
PflFI GACNNNGTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 5 cut(s) 111, 131, 362, 478, 608
PleI GAGTC 3 cut(s) 87, 313, 500
PpsI GAGTC 3 cut(s) 87, 313, 500
PpuMI RGGWCCY 1 cut(s) 530
PshBI ATTAAT 1 cut(s) 71
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 1 cut(s) 717
PspFI CCCAGC 1 cut(s) 182
PspGI CCWGG 1 cut(s) 717
PspN4I GGNNCC 6 cut(s) 187, 188, 531, 532, 591, 716
PspOMI GGGCCC 1 cut(s) 185
PspPI GGNCC 3 cut(s) 185, 186, 530
PspPPI RGGWCCY 1 cut(s) 530
PsuI RGATCY 1 cut(s) 318
PsyI GACNNNGTC 1 cut(s) 407
RsaI GTAC 3 cut(s) 647, 716, 761
RsaNI GTAC 3 cut(s) 646, 715, 760
SaqAI TTAA 2 cut(s) 71, 228
SatI GCNGC 5 cut(s) 110, 130, 361, 477, 607
Sau3AI GATC 5 cut(s) 318, 366, 441, 615, 696
Sau96I GGNCC 3 cut(s) 185, 186, 530
SchI GAGTC 3 cut(s) 87, 314, 500
ScrFI CCNGG 3 cut(s) 296, 534, 719
SduI GDGCHC 3 cut(s) 56, 189, 224
SetI ASST 6 cut(s) 272, 465, 519, 737, 807, 837
SfaNI GCATC 2 cut(s) 649, 745
SinI GGWCC 1 cut(s) 530
Sse9I AATT 2 cut(s) 38, 825
StyD4I CCNGG 3 cut(s) 294, 532, 717
TaaI ACNGT 1 cut(s) 58
TaiI ACGT 2 cut(s) 272, 737
TaqI TCGA 2 cut(s) 440, 504
TasI AATT 2 cut(s) 38, 825
TauI GCSGC 5 cut(s) 112, 132, 363, 479, 609
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 2 cut(s) 71, 228
Tru9I TTAA 2 cut(s) 71, 228
TscAI CASTG 3 cut(s) 61, 404, 473
TseFI GTSAC 1 cut(s) 371
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 1 cut(s) 51
TspGWI ACGGA 2 cut(s) 359, 405
TspRI CASTG 3 cut(s) 61, 404, 473
Tth111I GACNNNGTC 1 cut(s) 407
VneI GTGCAC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 530
VspI ATTAAT 1 cut(s) 71
XapI RAATTY 1 cut(s) 38
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.