RLG00000014840

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003321
Physical Location & Seq
Reverse (-)
58233661 .. 58235336
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014840

Sequence Viewer

Length: 1167 bp
ATGAACTCCGAAGTCGACCCAACTCGTAATCCATTTTCTTCTCCTTTTCGTTCTTCAAGGCTTGTGCGACTTTGTCTGGATGATATGAATGCCTCTGCTCAAAGGCGGGCTCTGTTAATGCGCGGCATGTTTACTCTCTTAGCTCACTATCTCGACGACGACGACGAAGATGACGAAGACGAAGACGAAGAGGACGAGGATCCCCAAGAACAAGCCGGAGAACAAGACCCACTCGCTAACCGGAAGCGGAGCCGCTCAACTACTCGAAACCCAAACGACGACGTTCCGCGCAAGGCCCACGCTGGTCCCCACTCGCCTTCGTTGACAAGCGACGCGGAGAGCCCATTTAAACCCGAAAATTCGCTCATCCCAAGAACAAAACCCGACACGTCGTTTATCCTCCACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTTAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCATGCCGACATCGACGCCAAGAAGGTGTGTGGTTTGGGTCCAGGAATGTCGTTCGCCAACGCGGTGAGGAGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGTACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGCGAGAGCGTGAAGAACGGAGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCGCGATGTTGATGCGTACCACGAGAAGATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATAATCCAGGTGGTTATTTGCTCCGGAGATGAGAAGTACCTGGAAAAAATTAGGGAGGTTCAGCTGGGAATTAAGGTTGAGAATGTTGTGTGTGTGGACGCTAAGGGGTTGGAGCTGAAAGATGATCATCTTCACCTGACCACTGAGGCTCAAGTTCAGTTGGGTCAGGTGCTGGCTGGTGCTTACCTTAAGCATTTTGGATCGGCTAATTCCCATCATCCTACCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

389

Amino Acids

43.28

Weight (kDa)

5.54

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 142 - 376 2.1e-94 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 825
AccB1I GGYRCC 2 cut(s) 700, 825
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 15
AccIII TCCGGA 1 cut(s) 959
AclWI GGATC 5 cut(s) 194, 207, 472, 547, 1144
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 1 cut(s) 358
AcyI GRCGYC 2 cut(s) 519, 618
AfaI GTAC 5 cut(s) 721, 758, 827, 872, 974
AfiI CCNNNNNNNGG 5 cut(s) 406, 412, 511, 580, 583
AflII CTTAAG 1 cut(s) 1124
AflIII ACRYGT 2 cut(s) 387, 843
AgsI TTSAA 1 cut(s) 57
AhdI GACNNNNNGTC 1 cut(s) 847
AjiI CACGTC 2 cut(s) 390, 846
AjnI CCWGG 4 cut(s) 643, 828, 942, 975
AluBI AGCT 3 cut(s) 143, 1000, 1051
AluI AGCT 3 cut(s) 143, 1000, 1051
Alw26I GTCTC 1 cut(s) 855
AlwI GGATC 5 cut(s) 194, 207, 472, 547, 1144
AlwNI CAGNNNCTG 1 cut(s) 1108
Aor13HI TCCGGA 1 cut(s) 959
AoxI GGCC 2 cut(s) 294, 490
ApoI RAATTY 1 cut(s) 358
Asp718I GGTACC 1 cut(s) 825
AspS9I GGNCC 3 cut(s) 295, 305, 641
AsuC2I CCSGG 1 cut(s) 407
AsuHPI GGTGA 4 cut(s) 563, 679, 781, 1061
AvaII GGWCC 2 cut(s) 305, 641
BamHI GGATCC 1 cut(s) 199
BanI GGYRCC 2 cut(s) 700, 825
BanII GRGCYC 2 cut(s) 112, 344
BauI CACGAG 1 cut(s) 875
BbsI GAAGAC 2 cut(s) 183, 189
BccI CCATC 2 cut(s) 877, 1158
BceAI ACGGC 4 cut(s) 505, 532, 688, 738
BcgI CGANNNNNNTGC 2 cut(s) 848, 882
BciT130I CCWGG 4 cut(s) 645, 830, 944, 977
BclI TGATCA 1 cut(s) 1060
BcnI CCSGG 1 cut(s) 407
BcoDI GTCTC 1 cut(s) 855
BfrI CTTAAG 1 cut(s) 1124
BglII AGATCT 1 cut(s) 429
BisI GCNGC 4 cut(s) 124, 253, 472, 588
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 4 cut(s) 125, 254, 473, 589
Bme1390I CCNGG 5 cut(s) 407, 645, 830, 944, 977
Bme18I GGWCC 2 cut(s) 305, 641
BmeRI GACNNNNNGTC 1 cut(s) 847
BmgBI CACGTC 2 cut(s) 390, 846
BmgT120I GGNCC 3 cut(s) 295, 305, 641
BmiI GGNNCC 6 cut(s) 201, 251, 307, 642, 702, 827
BmrFI CCNGG 5 cut(s) 407, 645, 830, 944, 977
BmrI ACTGGG 2 cut(s) 520, 589
BmsI GCATC 2 cut(s) 760, 856
BmuI ACTGGG 2 cut(s) 520, 589
BpiI GAAGAC 2 cut(s) 183, 189
Bpu10I CCTNAGC 1 cut(s) 1038
Bpu1102I GCTNAGC 1 cut(s) 537
BpuEI CTTGAG 1 cut(s) 1071
BpuMI CCSGG 1 cut(s) 407
BsaBI GATNNNNATC 1 cut(s) 1062
BsaHI GRCGYC 2 cut(s) 519, 618
BsaJI CCNNGG 2 cut(s) 487, 829
BsaWI WCCGGW 2 cut(s) 240, 959
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 5 cut(s) 406, 412, 511, 580, 583
Bse1I ACTGG 2 cut(s) 515, 584
Bse8I GATNNNNATC 1 cut(s) 1062
BseAI TCCGGA 1 cut(s) 959
BseBI CCWGG 4 cut(s) 645, 830, 944, 977
BseDI CCNNGG 2 cut(s) 487, 829
BseGI GGATG 3 cut(s) 85, 366, 1153
BseJI GATNNNNATC 1 cut(s) 1062
BseLI CCNNNNNNNGG 5 cut(s) 406, 412, 511, 580, 583
BseMII CTCAG 3 cut(s) 551, 866, 1071
BseNI ACTGG 2 cut(s) 515, 584
BsePI GCGCGC 1 cut(s) 779
BseRI GAGGAG 2 cut(s) 685, 816
BseYI CCCAGC 1 cut(s) 1000
BshFI GGCC 2 cut(s) 296, 492
BshNI GGYRCC 2 cut(s) 700, 825
BsiSI CCGG 5 cut(s) 216, 241, 407, 422, 960
BslFI GGGAC 2 cut(s) 291, 527
BslI CCNNNNNNNGG 5 cut(s) 406, 412, 511, 580, 583
BsmAI GTCTC 1 cut(s) 855
BsmBI CGTCTC 1 cut(s) 855
BsmFI GGGAC 2 cut(s) 291, 527
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 2 cut(s) 296, 492
Bsp1286I GDGCHC 2 cut(s) 112, 344
Bsp13I TCCGGA 1 cut(s) 959
Bsp143I GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
Bsp1720I GCTNAGC 1 cut(s) 537
BspANI GGCC 2 cut(s) 296, 492
BspCNI CTCAG 3 cut(s) 550, 865, 1072
BspEI TCCGGA 1 cut(s) 959
BspLI GGNNCC 6 cut(s) 201, 251, 307, 642, 702, 827
BspPI GGATC 5 cut(s) 194, 207, 472, 547, 1144
BspT107I GGYRCC 2 cut(s) 700, 825
BspTI CTTAAG 1 cut(s) 1124
BsrBI CCGCTC 1 cut(s) 255
BsrI ACTGG 2 cut(s) 515, 584
BssECI CCNNGG 2 cut(s) 487, 829
BssHII GCGCGC 1 cut(s) 779
BssMI GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
BssNI GRCGYC 2 cut(s) 519, 618
BssSI CACGAG 1 cut(s) 875
Bst2BI CACGAG 1 cut(s) 875
Bst2UI CCWGG 4 cut(s) 645, 830, 944, 977
Bst6I CTCTTC 2 cut(s) 183, 767
BstACI GRCGYC 2 cut(s) 519, 618
BstAFI CTTAAG 1 cut(s) 1124
BstC8I GCNNGC 3 cut(s) 108, 781, 1110
BstDEI CTNAG 5 cut(s) 139, 537, 852, 1038, 1080
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 3 cut(s) 85, 366, 1153
BstKTI GATC 8 cut(s) 202, 432, 480, 555, 729, 810, 1063, 1139
BstMAI GTCTC 1 cut(s) 855
BstMBI GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
BstMWI GCNNNNNNNGC 2 cut(s) 714, 748
BstNI CCWGG 4 cut(s) 645, 830, 944, 977
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 5 cut(s) 405, 643, 828, 942, 975
BstV2I GAAGAC 2 cut(s) 183, 189
BstX2I RGATCY 2 cut(s) 199, 429
BstYI RGATCY 2 cut(s) 199, 429
BsuRI GGCC 2 cut(s) 296, 492
BtgI CCRYGG 1 cut(s) 487
BtgZI GCGATG 1 cut(s) 873
BtrI CACGTC 2 cut(s) 390, 846
BtsCI GGATG 3 cut(s) 85, 366, 1153
BtsIMutI CAGTG 3 cut(s) 508, 577, 1077
Cac8I GCNNGC 3 cut(s) 108, 781, 1110
CaiI CAGNNNCTG 1 cut(s) 1108
Cfr13I GGNCC 3 cut(s) 295, 305, 641
CseI GACGC 4 cut(s) 341, 508, 626, 1043
Csp6I GTAC 5 cut(s) 720, 757, 826, 871, 973
CviAII CATG 5 cut(s) 127, 457, 605, 766, 911
CviQI GTAC 5 cut(s) 720, 757, 826, 871, 973
DdeI CTNAG 5 cut(s) 139, 537, 852, 1038, 1080
DpnI GATC 8 cut(s) 201, 431, 479, 554, 728, 809, 1062, 1138
DpnII GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
DraI TTTAAA 1 cut(s) 349
DriI GACNNNNNGTC 1 cut(s) 847
EaeI YGGCCR 1 cut(s) 490
Eam1104I CTCTTC 2 cut(s) 183, 767
Eam1105I GACNNNNNGTC 1 cut(s) 847
EarI CTCTTC 2 cut(s) 183, 767
EciI GGCGGA 1 cut(s) 548
Eco24I GRGCYC 2 cut(s) 112, 344
Eco47I GGWCC 2 cut(s) 305, 641
EcoRII CCWGG 4 cut(s) 643, 828, 942, 975
EcoT38I GRGCYC 2 cut(s) 112, 344
Esp3I CGTCTC 1 cut(s) 855
FaeI CATG 5 cut(s) 130, 460, 608, 769, 914
FaiI YATR 6 cut(s) 86, 128, 458, 606, 767, 912
FaqI GGGAC 2 cut(s) 291, 527
FatI CATG 5 cut(s) 126, 456, 604, 765, 910
FauI CCCGC 2 cut(s) 99, 454
FbaI TGATCA 1 cut(s) 1060
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 4 cut(s) 124, 253, 472, 588
FokI GGATG 3 cut(s) 92, 353, 1140
FriOI GRGCYC 2 cut(s) 112, 344
Fsp4HI GCNGC 4 cut(s) 124, 253, 472, 588
GluI GCNGC 4 cut(s) 124, 253, 472, 588
GsaI CCCAGC 1 cut(s) 1004
HaeIII GGCC 2 cut(s) 296, 492
HapII CCGG 5 cut(s) 216, 241, 407, 422, 960
HgaI GACGC 4 cut(s) 341, 508, 626, 1043
Hin1I GRCGYC 2 cut(s) 519, 618
Hin1II CATG 5 cut(s) 130, 460, 608, 769, 914
HincII GTYRAC 2 cut(s) 16, 324
HindII GTYRAC 2 cut(s) 16, 324
HinfI GANTC 1 cut(s) 416
HpaII CCGG 5 cut(s) 216, 241, 407, 422, 960
HphI GGTGA 4 cut(s) 563, 679, 781, 1061
Hpy166II GTNNAC 4 cut(s) 16, 132, 324, 1033
Hpy188I TCNGA 2 cut(s) 10, 532
Hpy188III TCNNGA 3 cut(s) 77, 152, 960
Hpy8I GTNNAC 4 cut(s) 16, 132, 324, 1033
HpyAV CCTTC 4 cut(s) 327, 558, 619, 936
HpyCH4IV ACGT 3 cut(s) 282, 389, 845
HpyF10VI GCNNNNNNNGC 2 cut(s) 714, 748
HpyF3I CTNAG 5 cut(s) 139, 537, 852, 1038, 1080
HpySE526I ACGT 3 cut(s) 282, 389, 845
Hsp92I GRCGYC 2 cut(s) 519, 618
Hsp92II CATG 5 cut(s) 130, 460, 608, 769, 914
Kpn2I TCCGGA 1 cut(s) 959
KpnI GGTACC 1 cut(s) 829
Ksp22I TGATCA 1 cut(s) 1060
Kzo9I GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
LmnI GCTCC 5 cut(s) 249, 424, 748, 962, 1048
LweI GCATC 2 cut(s) 760, 856
MaeII ACGT 3 cut(s) 282, 389, 845
MaeIII GTNAC 1 cut(s) 482
MalI GATC 8 cut(s) 201, 431, 479, 554, 728, 809, 1062, 1138
MbiI CCGCTC 1 cut(s) 255
MboI GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
MflI RGATCY 2 cut(s) 199, 429
MhlI GDGCHC 2 cut(s) 112, 344
MluCI AATT 4 cut(s) 358, 984, 1005, 1144
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 2 cut(s) 510, 1026
MroI TCCGGA 1 cut(s) 959
MseI TTAA 5 cut(s) 116, 348, 564, 1008, 1125
MspA1I CMGCKG 1 cut(s) 1000
MspCI CTTAAG 1 cut(s) 1124
MspI CCGG 5 cut(s) 216, 241, 407, 422, 960
MspR9I CCNGG 5 cut(s) 407, 645, 830, 944, 977
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 4 cut(s) 645, 830, 944, 977
MwoI GCNNNNNNNGC 2 cut(s) 714, 748
NciI CCSGG 1 cut(s) 407
NdeII GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
NlaIII CATG 5 cut(s) 130, 460, 608, 769, 914
NlaIV GGNNCC 6 cut(s) 201, 251, 307, 642, 702, 827
NmuCI GTSAC 1 cut(s) 482
NspI RCATGY 1 cut(s) 130
PauI GCGCGC 1 cut(s) 779
PcsI WCGNNNNNNNCGW 4 cut(s) 159, 162, 171, 192
PctI GAATGC 1 cut(s) 94
PflFI GACNNNGTC 2 cut(s) 72, 518
PfoI TCCNGGA 1 cut(s) 643
PkrI GCNGC 4 cut(s) 125, 254, 473, 589
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 4 cut(s) 643, 828, 942, 975
PspFI CCCAGC 1 cut(s) 1000
PspGI CCWGG 4 cut(s) 643, 828, 942, 975
PspN4I GGNNCC 6 cut(s) 201, 251, 307, 642, 702, 827
PspPI GGNCC 3 cut(s) 295, 305, 641
PstNI CAGNNNCTG 1 cut(s) 1108
PsuI RGATCY 2 cut(s) 199, 429
PsyI GACNNNGTC 2 cut(s) 72, 518
PteI GCGCGC 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 1000
RsaI GTAC 5 cut(s) 721, 758, 827, 872, 974
RsaNI GTAC 5 cut(s) 720, 757, 826, 871, 973
SalI GTCGAC 1 cut(s) 14
SaqAI TTAA 5 cut(s) 116, 348, 564, 1008, 1125
SatI GCNGC 4 cut(s) 124, 253, 472, 588
Sau3AI GATC 8 cut(s) 199, 429, 477, 552, 726, 807, 1060, 1136
Sau96I GGNCC 3 cut(s) 295, 305, 641
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 5 cut(s) 407, 645, 830, 944, 977
SduI GDGCHC 2 cut(s) 112, 344
SfaNI GCATC 2 cut(s) 760, 856
SinI GGWCC 2 cut(s) 305, 641
SmlI CTYRAG 2 cut(s) 1086, 1124
SmoI CTYRAG 2 cut(s) 1086, 1124
Sse9I AATT 4 cut(s) 358, 984, 1005, 1144
StyD4I CCNGG 5 cut(s) 405, 643, 828, 942, 975
TaiI ACGT 3 cut(s) 285, 392, 848
TaqI TCGA 5 cut(s) 15, 153, 265, 551, 615
TasI AATT 4 cut(s) 358, 984, 1005, 1144
TauI GCSGC 4 cut(s) 126, 255, 474, 590
Tru1I TTAA 5 cut(s) 116, 348, 564, 1008, 1125
Tru9I TTAA 5 cut(s) 116, 348, 564, 1008, 1125
TscAI CASTG 3 cut(s) 515, 584, 1084
TseFI GTSAC 1 cut(s) 482
Tsp45I GTSAC 1 cut(s) 482
TspDTI ATGAA 3 cut(s) 17, 101, 424
TspGWI ACGGA 3 cut(s) 470, 516, 813
TspRI CASTG 3 cut(s) 515, 584, 1084
Tth111I GACNNNGTC 2 cut(s) 72, 518
Vha464I CTTAAG 1 cut(s) 1124
VpaK11BI GGWCC 2 cut(s) 305, 641
XapI RAATTY 1 cut(s) 358
XceI RCATGY 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.