RchiOBHm_Chr6g0255421

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003321
Physical Location & Seq
Forward (+)
10747737 .. 10749980
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22908

Sequence Viewer

Length: 1188 bp
ATGAACTCCGAAGTCCACCCAATTCGTATTCCATTTTCTACTCGTTTCCCTTCAAACCTTAAGCGATATCTTCTCCAACGAATGAGAGACGAGGATGAAGAGCAGGCTCTTTTAACCCATTTGCTTACTGTCCTAGTTCACTATCTCAAAGACAAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAGGATTCTCAAGAACATGCCGGAGAACAACACCGACTCGCTAACCGGAAGCGGCGAAGAACTCGCCGTCGCGGCACAACTACACGAAGAAGACGAAACGACGGCGTTCCGCTCCAAGCATACGCTGGGCCCCACTCGCCTTCGTTGACAAGCGACGCGGAGAGGCCATTTAAACCCGAGAACTCGCTCATTCCAAGAACAAAACCCGACACGTCGTTTATCCTCCACCCGAAAAACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATCCTTTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGTGACCACGGCCACAACCACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACACCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCTCATTGAGAATGTGCGCCATGACCTTGCTTTGCCTTCACTTCCAATTATCCAGGTGGCTATTTGTTCTGGTGATGAAAAGTTCTTGGAGAAAATTAGGGAGGTTCAATTGGGGATGAAGGTTGAGAATGTTGTGTGTGTGGATGCCAAGGGATTGGAGCTCAAGGATGATCATCTTCACCTGACTACCAAGGCACAAGTTCAGTTGGGTCAGATGCTTGCTGATGCATACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

395

Amino Acids

44.73

Weight (kDa)

6.25

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 149 - 383 5.6e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 2 cut(s) 721, 846
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
AclWI GGATC 3 cut(s) 493, 568, 1165
AcoI YGGCCR 1 cut(s) 511
AcyI GRCGYC 1 cut(s) 540
AfaI GTAC 3 cut(s) 779, 848, 893
AfiI CCNNNNNNNGG 4 cut(s) 433, 532, 601, 604
AflII CTTAAG 1 cut(s) 59
AflIII ACRYGT 2 cut(s) 399, 864
AgsI TTSAA 2 cut(s) 54, 1019
AhdI GACNNNNNGTC 1 cut(s) 868
AjiI CACGTC 2 cut(s) 402, 867
AjnI CCWGG 2 cut(s) 849, 963
AluBI AGCT 2 cut(s) 1072, 1163
AluI AGCT 2 cut(s) 1072, 1163
Alw21I GWGCWC 1 cut(s) 1074
Alw26I GTCTC 2 cut(s) 81, 876
AlwI GGATC 3 cut(s) 493, 568, 1165
Ama87I CYCGRG 1 cut(s) 365
AoxI GGCC 3 cut(s) 317, 353, 511
ApaI GGGCCC 1 cut(s) 321
Asp718I GGTACC 1 cut(s) 846
AspLEI GCGC 6 cut(s) 613, 699, 731, 763, 802, 930
AspS9I GGNCC 3 cut(s) 317, 318, 662
AsuC2I CCSGG 2 cut(s) 428, 666
AsuHPI GGTGA 5 cut(s) 584, 700, 802, 995, 1082
AvaI CYCGRG 1 cut(s) 365
AvaII GGWCC 1 cut(s) 662
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 2 cut(s) 721, 846
BanII GRGCYC 2 cut(s) 321, 1074
BauI CACGAG 1 cut(s) 896
BbsI GAAGAC 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 1074
BccI CCATC 1 cut(s) 1179
BceAI ACGGC 6 cut(s) 240, 307, 526, 553, 709, 759
BciT130I CCWGG 2 cut(s) 851, 965
BclI TGATCA 1 cut(s) 1081
BcnI CCSGG 2 cut(s) 428, 666
BcoDI GTCTC 2 cut(s) 81, 876
BfaI CTAG 1 cut(s) 134
BfrI CTTAAG 1 cut(s) 59
BglI GCCNNNNNGGC 1 cut(s) 261
BglII AGATCT 1 cut(s) 450
BisI GCNGC 5 cut(s) 242, 262, 493, 609, 739
BlpI GCTNAGC 1 cut(s) 558
BlsI GCNGC 5 cut(s) 243, 263, 494, 610, 740
Bme1390I CCNGG 4 cut(s) 428, 666, 851, 965
Bme18I GGWCC 1 cut(s) 662
BmeRI GACNNNNNGTC 1 cut(s) 868
BmeT110I CYCGRG 1 cut(s) 365
BmgBI CACGTC 2 cut(s) 402, 867
BmgT120I GGNCC 3 cut(s) 317, 318, 662
BmiI GGNNCC 5 cut(s) 319, 320, 663, 723, 848
BmrFI CCNGG 4 cut(s) 428, 666, 851, 965
BmrI ACTGGG 2 cut(s) 541, 610
BmsI GCATC 5 cut(s) 781, 877, 1045, 1116, 1126
BmuI ACTGGG 2 cut(s) 541, 610
BpiI GAAGAC 1 cut(s) 286
Bpu1102I GCTNAGC 1 cut(s) 558
BpuEI CTTGAG 3 cut(s) 183, 1058, 1130
BpuMI CCSGG 2 cut(s) 428, 666
BsaBI GATNNNNATC 2 cut(s) 455, 1083
BsaHI GRCGYC 1 cut(s) 540
BsaJI CCNNGG 4 cut(s) 508, 850, 1059, 1101
BsaWI WCCGGW 1 cut(s) 234
BsaXI ACNNNNNCTCC 2 cut(s) 761, 791
Bsc4I CCNNNNNNNGG 4 cut(s) 433, 532, 601, 604
Bse1I ACTGG 2 cut(s) 536, 605
Bse8I GATNNNNATC 2 cut(s) 455, 1083
BseBI CCWGG 2 cut(s) 851, 965
BseDI CCNNGG 4 cut(s) 508, 850, 1059, 1101
BseGI GGATG 6 cut(s) 100, 456, 1032, 1060, 1084, 1174
BseJI GATNNNNATC 2 cut(s) 455, 1083
BseLI CCNNNNNNNGG 4 cut(s) 433, 532, 601, 604
BseMII CTCAG 2 cut(s) 572, 887
BseNI ACTGG 2 cut(s) 536, 605
BseRI GAGGAG 7 cut(s) 185, 188, 191, 194, 197, 200, 203
BseSI GKGCMC 1 cut(s) 321
BseYI CCCAGC 1 cut(s) 314
Bsh1236I CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
BshFI GGCC 3 cut(s) 319, 355, 513
BshNI GGYRCC 2 cut(s) 721, 846
BsiHKAI GWGCWC 1 cut(s) 1074
BsiHKCI CYCGRG 1 cut(s) 365
BsiSI CCGG 5 cut(s) 210, 235, 428, 443, 665
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 4 cut(s) 433, 532, 601, 604
BsmAI GTCTC 2 cut(s) 81, 876
BsmBI CGTCTC 2 cut(s) 81, 876
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 3 cut(s) 319, 355, 513
BsoBI CYCGRG 1 cut(s) 365
Bsp120I GGGCCC 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 321, 1074
Bsp143I GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
Bsp1720I GCTNAGC 1 cut(s) 558
BspANI GGCC 3 cut(s) 319, 355, 513
BspCNI CTCAG 2 cut(s) 571, 886
BspFNI CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
BspLI GGNNCC 5 cut(s) 319, 320, 663, 723, 848
BspPI GGATC 3 cut(s) 493, 568, 1165
BspQI GCTCTTC 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 721, 846
BspTI CTTAAG 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 301
BsrI ACTGG 2 cut(s) 536, 605
BssECI CCNNGG 4 cut(s) 508, 850, 1059, 1101
BssMI GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
BssNI GRCGYC 1 cut(s) 540
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 2 cut(s) 1059, 1101
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 2 cut(s) 851, 965
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 4 cut(s) 93, 156, 162, 788
BstACI GRCGYC 1 cut(s) 540
BstAFI CTTAAG 1 cut(s) 59
BstC8I GCNNGC 2 cut(s) 105, 1131
BstDEI CTNAG 2 cut(s) 558, 873
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 6 cut(s) 100, 456, 1032, 1060, 1084, 1174
BstFNI CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
BstHHI GCGC 6 cut(s) 613, 699, 731, 763, 802, 930
BstKTI GATC 7 cut(s) 453, 501, 576, 750, 831, 1084, 1160
BstMAI GTCTC 2 cut(s) 81, 876
BstMBI GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
BstMWI GCNNNNNNNGC 4 cut(s) 261, 325, 735, 769
BstNI CCWGG 2 cut(s) 851, 965
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 4 cut(s) 426, 664, 849, 963
BstSLI GKGCMC 1 cut(s) 321
BstUI CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
BstV2I GAAGAC 1 cut(s) 286
BstX2I RGATCY 1 cut(s) 450
BstXI CCANNNNNNTGG 1 cut(s) 1066
BstYI RGATCY 1 cut(s) 450
BsuRI GGCC 3 cut(s) 319, 355, 513
BtgI CCRYGG 1 cut(s) 508
BtrI CACGTC 2 cut(s) 402, 867
BtsCI GGATG 6 cut(s) 100, 456, 1032, 1060, 1084, 1174
BtsIMutI CAGTG 2 cut(s) 529, 598
Cac8I GCNNGC 2 cut(s) 105, 1131
CfoI GCGC 6 cut(s) 613, 699, 731, 763, 802, 930
Cfr13I GGNCC 3 cut(s) 317, 318, 662
CseI GACGC 2 cut(s) 353, 529
Csp6I GTAC 3 cut(s) 778, 847, 892
CviAII CATG 6 cut(s) 206, 478, 787, 878, 904, 932
CviQI GTAC 3 cut(s) 778, 847, 892
DdeI CTNAG 2 cut(s) 558, 873
DpnI GATC 7 cut(s) 452, 500, 575, 749, 830, 1083, 1159
DpnII GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
DraI TTTAAA 1 cut(s) 361
DriI GACNNNNNGTC 1 cut(s) 868
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 4 cut(s) 93, 156, 162, 788
Eam1105I GACNNNNNGTC 1 cut(s) 868
EarI CTCTTC 4 cut(s) 93, 156, 162, 788
EciI GGCGGA 1 cut(s) 569
Ecl136II GAGCTC 1 cut(s) 1072
Eco130I CCWWGG 2 cut(s) 1059, 1101
Eco24I GRGCYC 2 cut(s) 321, 1074
Eco32I GATATC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 662
Eco53kI GAGCTC 1 cut(s) 1072
Eco88I CYCGRG 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 1072
EcoO109I RGGNCCY 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 849, 963
EcoRV GATATC 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 1059, 1101
EcoT22I ATGCAT 1 cut(s) 1141
EcoT38I GRGCYC 2 cut(s) 321, 1074
ErhI CCWWGG 2 cut(s) 1059, 1101
Esp3I CGTCTC 2 cut(s) 81, 876
FaeI CATG 6 cut(s) 209, 481, 790, 881, 907, 935
FaiI YATR 8 cut(s) 207, 310, 479, 788, 879, 905, 933, 1141
FaqI GGGAC 1 cut(s) 548
FatI CATG 6 cut(s) 205, 477, 786, 877, 903, 931
FauI CCCGC 1 cut(s) 475
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 5 cut(s) 242, 262, 493, 609, 739
FokI GGATG 6 cut(s) 107, 443, 1039, 1067, 1091, 1161
FriOI GRGCYC 2 cut(s) 321, 1074
Fsp4HI GCNGC 5 cut(s) 242, 262, 493, 609, 739
FspBI CTAG 1 cut(s) 134
GlaI GCGC 6 cut(s) 612, 698, 730, 762, 801, 929
GluI GCNGC 5 cut(s) 242, 262, 493, 609, 739
GsaI CCCAGC 1 cut(s) 318
HaeIII GGCC 3 cut(s) 319, 355, 513
HapII CCGG 5 cut(s) 210, 235, 428, 443, 665
HgaI GACGC 2 cut(s) 353, 529
HhaI GCGC 6 cut(s) 613, 699, 731, 763, 802, 930
Hin1I GRCGYC 1 cut(s) 540
Hin1II CATG 6 cut(s) 209, 481, 790, 881, 907, 935
Hin6I GCGC 6 cut(s) 611, 697, 729, 761, 800, 928
HinP1I GCGC 6 cut(s) 611, 697, 729, 761, 800, 928
HincII GTYRAC 1 cut(s) 336
HindII GTYRAC 1 cut(s) 336
HinfI GANTC 3 cut(s) 194, 225, 437
HpaII CCGG 5 cut(s) 210, 235, 428, 443, 665
HphI GGTGA 5 cut(s) 584, 700, 802, 995, 1082
Hpy166II GTNNAC 3 cut(s) 16, 139, 336
Hpy188I TCNGA 3 cut(s) 10, 553, 1125
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 3 cut(s) 16, 139, 336
Hpy99I CGWCG 7 cut(s) 261, 293, 347, 406, 545, 553, 871
HpyAV CCTTC 6 cut(s) 60, 339, 579, 640, 957, 1024
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 2 cut(s) 401, 866
HpyCH4V TGCA 1 cut(s) 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 261, 325, 735, 769
HpyF3I CTNAG 2 cut(s) 558, 873
HpySE526I ACGT 2 cut(s) 401, 866
Hsp92I GRCGYC 1 cut(s) 540
Hsp92II CATG 6 cut(s) 209, 481, 790, 881, 907, 935
HspAI GCGC 6 cut(s) 611, 697, 729, 761, 800, 928
KpnI GGTACC 1 cut(s) 850
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
LguI GCTCTTC 1 cut(s) 93
LmnI GCTCC 4 cut(s) 306, 445, 769, 1069
LweI GCATC 5 cut(s) 781, 877, 1045, 1116, 1126
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 401, 866
MaeIII GTNAC 1 cut(s) 503
MalI GATC 7 cut(s) 452, 500, 575, 749, 830, 1083, 1159
MbiI CCGCTC 1 cut(s) 301
MboI GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
MfeI CAATTG 1 cut(s) 1019
MflI RGATCY 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 321, 1074
MluCI AATT 5 cut(s) 21, 957, 1005, 1019, 1165
MlyI GAGTC 2 cut(s) 219, 446
MmeI TCCRAC 2 cut(s) 100, 531
Mph1103I ATGCAT 1 cut(s) 1141
MseI TTAA 3 cut(s) 60, 113, 360
MspCI CTTAAG 1 cut(s) 59
MspI CCGG 5 cut(s) 210, 235, 428, 443, 665
MspR9I CCNGG 4 cut(s) 428, 666, 851, 965
MunI CAATTG 1 cut(s) 1019
MvaI CCWGG 2 cut(s) 851, 965
MvnI CGCG 8 cut(s) 261, 347, 613, 686, 699, 731, 763, 802
MwoI GCNNNNNNNGC 4 cut(s) 261, 325, 735, 769
NciI CCSGG 2 cut(s) 428, 666
NdeII GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
NlaIII CATG 6 cut(s) 209, 481, 790, 881, 907, 935
NlaIV GGNNCC 5 cut(s) 319, 320, 663, 723, 848
NmuCI GTSAC 1 cut(s) 503
NsiI ATGCAT 1 cut(s) 1141
NspI RCATGY 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 93
PcsI WCGNNNNNNNCGW 2 cut(s) 280, 633
PfeI GAWTC 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 664
PkrI GCNGC 5 cut(s) 243, 263, 494, 610, 740
PleI GAGTC 2 cut(s) 219, 445
PpsI GAGTC 2 cut(s) 219, 445
Psp124BI GAGCTC 1 cut(s) 1074
Psp6I CCWGG 2 cut(s) 849, 963
PspFI CCCAGC 1 cut(s) 314
PspGI CCWGG 2 cut(s) 849, 963
PspN4I GGNNCC 5 cut(s) 319, 320, 663, 723, 848
PspOMI GGGCCC 1 cut(s) 317
PspPI GGNCC 3 cut(s) 317, 318, 662
PsuI RGATCY 1 cut(s) 450
PsyI GACNNNGTC 1 cut(s) 539
RsaI GTAC 3 cut(s) 779, 848, 893
RsaNI GTAC 3 cut(s) 778, 847, 892
SacI GAGCTC 1 cut(s) 1074
SapI GCTCTTC 1 cut(s) 93
SaqAI TTAA 3 cut(s) 60, 113, 360
SatI GCNGC 5 cut(s) 242, 262, 493, 609, 739
Sau3AI GATC 7 cut(s) 450, 498, 573, 747, 828, 1081, 1157
Sau96I GGNCC 3 cut(s) 317, 318, 662
SchI GAGTC 2 cut(s) 219, 446
ScrFI CCNGG 4 cut(s) 428, 666, 851, 965
SduI GDGCHC 2 cut(s) 321, 1074
SfaNI GCATC 5 cut(s) 781, 877, 1045, 1116, 1126
SinI GGWCC 1 cut(s) 662
SmlI CTYRAG 4 cut(s) 59, 198, 1073, 1145
SmoI CTYRAG 4 cut(s) 59, 198, 1073, 1145
Sse9I AATT 5 cut(s) 21, 957, 1005, 1019, 1165
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 1074
StyD4I CCNGG 4 cut(s) 426, 664, 849, 963
StyI CCWWGG 2 cut(s) 1059, 1101
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 2 cut(s) 404, 869
TaqI TCGA 2 cut(s) 572, 636
TasI AATT 5 cut(s) 21, 957, 1005, 1019, 1165
TauI GCSGC 5 cut(s) 244, 264, 495, 611, 741
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 3 cut(s) 60, 113, 360
Tru9I TTAA 3 cut(s) 60, 113, 360
TscAI CASTG 2 cut(s) 536, 605
TseFI GTSAC 1 cut(s) 503
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 5 cut(s) 17, 111, 445, 1002, 1043
TspGWI ACGGA 2 cut(s) 491, 537
TspRI CASTG 2 cut(s) 536, 605
Tth111I GACNNNGTC 1 cut(s) 539
Vha464I CTTAAG 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 662
XceI RCATGY 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 311
XspI CTAG 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 1141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.