FvH4_2g33790

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003321
Physical Location & Seq
Reverse (-)
25237714 .. 25239561
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33790.t1

Sequence Viewer

Length: 804 bp
ATGGCGACTAGTGCCAAGACTGCCATCTCAAACCTGGTTCTGCTATTCTCTGCAGTCCTAACGACCCAATTCAACTTAGGTTCATCTGTTGCTTCTCCCGGGAGCATCTTCATCCTCGCCGGTCAGAGTAACATGGCCGGGAGAGGTGGCGTCAACGACGGCAAGTGGGACGGAAACGTTCCTCCGGAGTGCGCACCGAACCTTTCAATTCTCAGACTGAATGCTCACCTGGTGTGGGAAGAAGCATATGAGCCACTCCACGCTGACATTGATGTTGGCAAGACGTGTGGGGTTGGTCCAGGTATGGCTTTTGCCAACGAGGTCCTCCGAGCCAAGGGCGGAGTTTTGGGTCTGGTTCCTTGCGCCGTGGGCGGGACTACTATCGGCCAGTGGGCTCGAGGGAGTCAGTTGTATACTCAGATGGTGAGACGAGCAAGTGAGTCGGTGAAGGCAGGTGGAACTATCCGAGCAGTTTTATGGTACCAGGGAGAGAGTGACACCGTCAATAGTGCTGATGCCGAGGCTTATAAAGCTAATTTTGAGAGGCTTGTAACTGATATACGCTCCGATCTCCAAAACCCTAATCTTCCTGTTATTCAGGTAGCACTGGCATCAGGAGAAGGGCAGTTCATAGATATAGTGAGGAAGGCACAGCTGGAAACGACGTTGCCTAATGTGAAATGTGTCGACGCTAAAGGATTGCCTCTGAAACCGGATAATCTCCACCTTACTACAATGTCGGAGGTCCATCTGGGGATCAAGCTAGCAAATGCTTTTCTGGCTTCGTTTGGTTATCAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

268

Amino Acids

28.27

Weight (kDa)

6.09

Isoelectric Point (pI)

27.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 34 - 260 2.4e-95 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
AarI CACCTGC 1 cut(s) 443
Acc16I TGCGCA 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 443
Acc65I GGTACC 1 cut(s) 480
AccB1I GGYRCC 1 cut(s) 480
AccI GTMKAC 2 cut(s) 413, 687
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 2 cut(s) 339, 372
AclI AACGTT 1 cut(s) 177
AclWI GGATC 1 cut(s) 764
AcoI YGGCCR 2 cut(s) 135, 385
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 1 cut(s) 232
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 3 cut(s) 235, 334, 372
AflIII ACRYGT 1 cut(s) 284
AgsI TTSAA 2 cut(s) 73, 207
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 285
AjnI CCWGG 4 cut(s) 33, 228, 298, 483
AluBI AGCT 3 cut(s) 533, 655, 763
AluI AGCT 3 cut(s) 533, 655, 763
Alw26I GTCTC 1 cut(s) 421
AlwI GGATC 1 cut(s) 764
Ama87I CYCGRG 2 cut(s) 98, 396
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 2 cut(s) 135, 385
Asp718I GGTACC 1 cut(s) 480
AspLEI GCGC 2 cut(s) 194, 365
AspS9I GGNCC 3 cut(s) 296, 322, 745
AsuC2I CCSGG 3 cut(s) 99, 100, 139
AsuHPI GGTGA 3 cut(s) 218, 436, 457
AsuNHI GCTAGC 1 cut(s) 763
AvaI CYCGRG 2 cut(s) 98, 396
AvaII GGWCC 3 cut(s) 296, 322, 745
BanI GGYRCC 1 cut(s) 480
BanII GRGCYC 1 cut(s) 397
BccI CCATC 3 cut(s) 32, 415, 756
BceAI ACGGC 2 cut(s) 175, 350
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 4 cut(s) 35, 230, 300, 485
BcnI CCSGG 3 cut(s) 99, 100, 139
BcoDI GTCTC 1 cut(s) 421
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 2 cut(s) 9, 764
BfmI CTRYAG 1 cut(s) 51
BfuAI ACCTGC 1 cut(s) 443
Bme1390I CCNGG 7 cut(s) 35, 99, 100, 139, 230, 300, 485
Bme18I GGWCC 3 cut(s) 296, 322, 745
BmeT110I CYCGRG 2 cut(s) 98, 396
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 296, 322, 745
BmiI GGNNCC 2 cut(s) 357, 482
BmrFI CCNGG 7 cut(s) 35, 99, 100, 139, 230, 300, 485
BmsI GCATC 3 cut(s) 114, 505, 620
BmtI GCTAGC 1 cut(s) 767
BpuMI CCSGG 3 cut(s) 99, 100, 139
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 5 cut(s) 98, 333, 366, 484, 519
BsaWI WCCGGW 2 cut(s) 184, 712
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 334, 372
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 2 cut(s) 388, 612
BseAI TCCGGA 1 cut(s) 184
BseBI CCWGG 4 cut(s) 35, 230, 300, 485
BseDI CCNNGG 5 cut(s) 98, 333, 366, 484, 519
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 3 cut(s) 235, 334, 372
BseMII CTCAG 2 cut(s) 226, 431
BseNI ACTGG 2 cut(s) 388, 612
BshFI GGCC 2 cut(s) 137, 387
BshNI GGYRCC 1 cut(s) 480
BsiHKCI CYCGRG 2 cut(s) 98, 396
BsiSI CCGG 5 cut(s) 99, 120, 138, 185, 713
BslFI GGGAC 2 cut(s) 182, 388
BslI CCNNNNNNNGG 3 cut(s) 235, 334, 372
BsmAI GTCTC 1 cut(s) 421
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 2 cut(s) 182, 388
BsmI GAATGC 1 cut(s) 226
BsnI GGCC 2 cut(s) 137, 387
BsoBI CYCGRG 2 cut(s) 98, 396
Bsp1286I GDGCHC 1 cut(s) 397
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 2 cut(s) 568, 756
BspACI CCGC 2 cut(s) 339, 372
BspANI GGCC 2 cut(s) 137, 387
BspCNI CTCAG 2 cut(s) 225, 430
BspEI TCCGGA 1 cut(s) 184
BspLI GGNNCC 2 cut(s) 357, 482
BspMAI CTGCAG 1 cut(s) 55
BspMI ACCTGC 1 cut(s) 443
BspOI GCTAGC 1 cut(s) 767
BspPI GGATC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 2 cut(s) 388, 612
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 98, 333, 366, 484, 519
BssMI GATC 2 cut(s) 568, 756
BssNAI GTATAC 1 cut(s) 414
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 333
Bst1107I GTATAC 1 cut(s) 414
Bst2UI CCWGG 4 cut(s) 35, 230, 300, 485
Bst4CI ACNGT 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 3 cut(s) 76, 212, 417
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 1 cut(s) 111
BstHHI GCGC 2 cut(s) 194, 365
BstKTI GATC 2 cut(s) 571, 759
BstMAI GTCTC 1 cut(s) 421
BstMBI GATC 2 cut(s) 568, 756
BstMWI GCNNNNNNNGC 5 cut(s) 11, 20, 369, 530, 779
BstNI CCWGG 4 cut(s) 35, 230, 300, 485
BstSCI CCNGG 7 cut(s) 33, 97, 98, 137, 228, 298, 483
BstSFI CTRYAG 1 cut(s) 51
BstZ17I GTATAC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 137, 387
BtgI CCRYGG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 285
BtsCI GGATG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 395, 605
BveI ACCTGC 1 cut(s) 443
Cac8I GCNNGC 1 cut(s) 765
CfoI GCGC 2 cut(s) 194, 365
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 296, 322, 745
Cfr9I CCCGGG 1 cut(s) 98
CseI GACGC 2 cut(s) 139, 698
CsiI ACCWGGT 2 cut(s) 33, 228
Csp6I GTAC 1 cut(s) 481
CspCI CAANNNNNGTGG 2 cut(s) 268, 303
CviAII CATG 1 cut(s) 133
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 3 cut(s) 76, 212, 417
DpnI GATC 2 cut(s) 570, 758
DpnII GATC 2 cut(s) 568, 756
DraIII CACNNNGTG 1 cut(s) 232
EaeI YGGCCR 2 cut(s) 135, 385
EciI GGCGGA 1 cut(s) 354
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 397
Eco47I GGWCC 3 cut(s) 296, 322, 745
Eco88I CYCGRG 2 cut(s) 98, 396
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 4 cut(s) 33, 228, 298, 483
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 1 cut(s) 136
FaqI GGGAC 2 cut(s) 182, 388
FatI CATG 1 cut(s) 132
FauI CCCGC 1 cut(s) 365
FauNDI CATATG 1 cut(s) 247
FblI GTMKAC 2 cut(s) 413, 687
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 397
FspAI RTGCGCAY 1 cut(s) 193
FspBI CTAG 2 cut(s) 9, 764
FspI TGCGCA 1 cut(s) 193
GlaI GCGC 2 cut(s) 193, 364
HaeIII GGCC 2 cut(s) 137, 387
HapII CCGG 5 cut(s) 99, 120, 138, 185, 713
HgaI GACGC 2 cut(s) 139, 698
HhaI GCGC 2 cut(s) 194, 365
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 136
Hin6I GCGC 2 cut(s) 192, 363
HinP1I GCGC 2 cut(s) 192, 363
HincII GTYRAC 2 cut(s) 154, 688
HindII GTYRAC 2 cut(s) 154, 688
HinfI GANTC 2 cut(s) 403, 440
HpaII CCGG 5 cut(s) 99, 120, 138, 185, 713
HphI GGTGA 3 cut(s) 218, 436, 457
Hpy166II GTNNAC 3 cut(s) 154, 414, 688
Hpy188I TCNGA 8 cut(s) 126, 215, 329, 420, 467, 568, 708, 742
Hpy188III TCNNGA 2 cut(s) 185, 615
Hpy8I GTNNAC 3 cut(s) 154, 414, 688
Hpy99I CGWCG 3 cut(s) 161, 667, 692
HpyAV CCTTC 3 cut(s) 442, 614, 640
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4IV ACGT 3 cut(s) 177, 284, 665
HpyCH4V TGCA 1 cut(s) 53
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 20, 369, 530, 779
HpyF3I CTNAG 3 cut(s) 76, 212, 417
HpySE526I ACGT 3 cut(s) 177, 284, 665
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 136
HspAI GCGC 2 cut(s) 192, 363
Kpn2I TCCGGA 1 cut(s) 184
KpnI GGTACC 1 cut(s) 484
Kzo9I GATC 2 cut(s) 568, 756
LmnI GCTCC 2 cut(s) 102, 569
LweI GCATC 3 cut(s) 114, 505, 620
MabI ACCWGGT 2 cut(s) 33, 228
MaeI CTAG 2 cut(s) 9, 764
MaeII ACGT 3 cut(s) 177, 284, 665
MaeIII GTNAC 3 cut(s) 128, 494, 550
MalI GATC 2 cut(s) 570, 758
MboI GATC 2 cut(s) 568, 756
MboII GAAGA 3 cut(s) 100, 251, 578
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 3 cut(s) 68, 207, 535
MlyI GAGTC 2 cut(s) 412, 449
MmeI TCCRAC 1 cut(s) 720
MroI TCCGGA 1 cut(s) 184
MseI TTAA 1 cut(s) 802
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 5 cut(s) 99, 120, 138, 185, 713
MspR9I CCNGG 7 cut(s) 35, 99, 100, 139, 230, 300, 485
Mva1269I GAATGC 1 cut(s) 226
MvaI CCWGG 4 cut(s) 35, 230, 300, 485
MwoI GCNNNNNNNGC 5 cut(s) 11, 20, 369, 530, 779
NciI CCSGG 3 cut(s) 99, 100, 139
NdeI CATATG 1 cut(s) 247
NdeII GATC 2 cut(s) 568, 756
NheI GCTAGC 1 cut(s) 763
NlaIII CATG 1 cut(s) 136
NlaIV GGNNCC 2 cut(s) 357, 482
NmeAIII GCCGAG 1 cut(s) 544
NmuCI GTSAC 1 cut(s) 494
NsbI TGCGCA 1 cut(s) 193
PaeR7I CTCGAG 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 443
PctI GAATGC 1 cut(s) 226
PflFI GACNNNGTC 1 cut(s) 500
PleI GAGTC 2 cut(s) 411, 448
PpsI GAGTC 2 cut(s) 411, 448
PpuMI RGGWCCY 1 cut(s) 322
PsiI TTATAA 1 cut(s) 528
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 4 cut(s) 33, 228, 298, 483
PspGI CCWGG 4 cut(s) 33, 228, 298, 483
PspN4I GGNNCC 2 cut(s) 357, 482
PspPI GGNCC 3 cut(s) 296, 322, 745
PspPPI RGGWCCY 1 cut(s) 322
PspXI VCTCGAGB 1 cut(s) 396
PstI CTGCAG 1 cut(s) 55
PsyI GACNNNGTC 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 655
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 1 cut(s) 802
Sau3AI GATC 2 cut(s) 568, 756
Sau96I GGNCC 3 cut(s) 296, 322, 745
SchI GAGTC 2 cut(s) 412, 449
ScrFI CCNGG 7 cut(s) 35, 99, 100, 139, 230, 300, 485
SduI GDGCHC 1 cut(s) 397
SexAI ACCWGGT 2 cut(s) 33, 228
SfaNI GCATC 3 cut(s) 114, 505, 620
SfcI CTRYAG 1 cut(s) 51
Sfr274I CTCGAG 1 cut(s) 396
SinI GGWCC 3 cut(s) 296, 322, 745
SlaI CTCGAG 1 cut(s) 396
SmaI CCCGGG 1 cut(s) 100
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
SpeI ACTAGT 1 cut(s) 8
Sse9I AATT 3 cut(s) 68, 207, 535
SsiI CCGC 2 cut(s) 339, 372
SspMI CTAG 2 cut(s) 9, 764
StyD4I CCNGG 7 cut(s) 33, 97, 98, 137, 228, 298, 483
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 1 cut(s) 502
TaiI ACGT 3 cut(s) 180, 287, 668
TaqI TCGA 2 cut(s) 397, 687
TasI AATT 3 cut(s) 68, 207, 535
Tru1I TTAA 1 cut(s) 802
Tru9I TTAA 1 cut(s) 802
TscAI CASTG 2 cut(s) 395, 612
TseFI GTSAC 1 cut(s) 494
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 3 cut(s) 72, 100, 619
TspGWI ACGGA 1 cut(s) 186
TspMI CCCGGG 1 cut(s) 98
TspRI CASTG 2 cut(s) 395, 612
Tth111I GACNNNGTC 1 cut(s) 500
VpaK11BI GGWCC 3 cut(s) 296, 322, 745
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XhoI CTCGAG 1 cut(s) 396
XmaI CCCGGG 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 413, 687
XspI CTAG 2 cut(s) 9, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.