FvH4_2g05290

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003321
Physical Location & Seq
Forward (+)
4363880 .. 4365870
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05290.t2

Sequence Viewer

Length: 1155 bp
ATGACTACTTCCGACTCCGACGACCCAACTCGTGGTCCATTTTCTTCTCCCTGCTTCAACTTGCTCTGGCGACATTATCAGCAAGAAATGAAAGACGATGAAGAGCGTCAAGCTCGGATAACCTCCTTCCTTGCTCTCGTGACTCAGTATCTCAAGCTCAAAGAGGAAGAAGATGCCATACAACAACGCCGACTCGCTAGCCGGAAGCGGCGAAGAGCTCCCCGCCGGCGCGGCACAACTACACAAAGACGAAACGACGGCGTTCCGCGCGTGGTGCCCTACGCTGGGCCCCACTCGCCTTCGTTGACAAGCGACGCGGAGAGCGCTTTTAAACCCGAGAACTCGCTCATTTTAAGAACAAAACGCGACACGTCGTCTATCCTCCTCCCACCGAAAAACCCGGAAATGGAATCGTCGCCGGAGCAGATCTTCATTCTCTCAGGTCAGAGCAACATGGCGGGCCGTGGCGGCGTGATCCGTGACCACCACCACCAGCACTGGGACGGCGTCGTTCCGTCCGAGTCTCAGCCCGATCCTTCGATCCTCCGCCTCAGCGTTCACCTCCGCTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATAGACGCCAAGAAGGTGTGCGGCTTGGGTCCGGGAATGTCGTTTGCCAACGCGGTCAGGGGGCGCGTGGAGGGGAGGATGGGGCTGGTGCCGTGCGCGGTGGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTATGAGAATATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAAGGGGAGAGCGACACGTCGTCTGAGCATGATGTTGATGCGTACCACGGGAACATGGTTAGGCTCATTGACAATGTTCGCCAGGACCTTGCTTTGCCTTCACTTCCAATCATCCAGGTGGCTATTTGTTCTGGAGATGAGAAGTACTTGGAGAAAATTAGGGAGGTACAATTGGGGATGAAGGTTAAGAATGTTGTGTGTGTGGATGCTAAGGGATTGGAGCTGAAGGAGGATCATCTTCACCTGACCACCAAGGCTCAAGTTCAGTTGGGTCAGATGCTGGCTGATGCTTACCTCAAGCATTTTGGATCAGCTGATTCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

385

Amino Acids

43.02

Weight (kDa)

8.21

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 101 - 332 4.7e-95 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 813
AccB1I GGYRCC 3 cut(s) 274, 688, 813
AccB7I CCANNNNNTGG 1 cut(s) 32
AclWI GGATC 5 cut(s) 469, 527, 535, 1056, 1132
AcuI CTGAAG 1 cut(s) 1061
AcyI GRCGYC 2 cut(s) 507, 606
AfaI GTAC 4 cut(s) 815, 860, 962, 984
AfeI AGCGCT 1 cut(s) 325
AfiI CCNNNNNNNGG 7 cut(s) 32, 284, 285, 406, 499, 568, 571
AflIII ACRYGT 2 cut(s) 369, 831
AgsI TTSAA 1 cut(s) 58
AhdI GACNNNNNGTC 2 cut(s) 373, 835
AjiI CACGTC 2 cut(s) 372, 834
AjnI CCWGG 2 cut(s) 897, 930
AluBI AGCT 5 cut(s) 113, 157, 218, 1039, 1130
AluI AGCT 5 cut(s) 113, 157, 218, 1039, 1130
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 5 cut(s) 469, 527, 535, 1056, 1132
AlwNI CAGNNNCTG 1 cut(s) 1096
Ama87I CYCGRG 1 cut(s) 335
Aor51HI AGCGCT 1 cut(s) 325
AoxI GGCC 2 cut(s) 287, 460
ApaI GGGCCC 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 813
AspLEI GCGC 8 cut(s) 231, 270, 326, 580, 666, 698, 730, 769
AspS9I GGNCC 6 cut(s) 35, 287, 288, 460, 629, 901
AsuC2I CCSGG 2 cut(s) 401, 633
AsuHPI GGTGA 3 cut(s) 551, 769, 1049
AsuNHI GCTAGC 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 3 cut(s) 35, 629, 901
BaeGI GKGCMC 2 cut(s) 279, 291
BanI GGYRCC 3 cut(s) 274, 688, 813
BanII GRGCYC 2 cut(s) 220, 291
BarI GAAGNNNNNNTAC 2 cut(s) 162, 194
BauI CACGAG 2 cut(s) 30, 137
Bbv12I GWGCWC 1 cut(s) 220
BbvCI CCTCAGC 1 cut(s) 551
BccI CCATC 2 cut(s) 673, 1146
BceAI ACGGC 5 cut(s) 274, 447, 520, 676, 726
BciT130I CCWGG 2 cut(s) 899, 932
BcnI CCSGG 2 cut(s) 401, 633
BcoDI GTCTC 1 cut(s) 528
BfaI CTAG 1 cut(s) 198
BfoI RGCGCY 1 cut(s) 327
BglI GCCNNNNNGGC 2 cut(s) 231, 468
BglII AGATCT 1 cut(s) 426
BisI GCNGC 6 cut(s) 209, 232, 469, 576, 622, 706
BlsI GCNGC 6 cut(s) 210, 233, 470, 577, 623, 707
BmcAI AGTACT 1 cut(s) 962
Bme1390I CCNGG 4 cut(s) 401, 633, 899, 932
Bme18I GGWCC 3 cut(s) 35, 629, 901
BmeRI GACNNNNNGTC 2 cut(s) 373, 835
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 2 cut(s) 372, 834
BmgT120I GGNCC 6 cut(s) 35, 287, 288, 460, 629, 901
BmiI GGNNCC 6 cut(s) 276, 289, 290, 630, 690, 815
BmrFI CCNGG 4 cut(s) 401, 633, 899, 932
BmrI ACTGGG 1 cut(s) 508
BmsI GCATC 6 cut(s) 163, 748, 844, 1012, 1083, 1093
BmtI GCTAGC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 508
BpmI CTGGAG 1 cut(s) 969
Bpu10I CCTNAGC 2 cut(s) 551, 1026
BpuEI CTTGAG 3 cut(s) 137, 1059, 1097
BpuMI CCSGG 2 cut(s) 401, 633
BsaBI GATNNNNATC 1 cut(s) 1137
BsaHI GRCGYC 2 cut(s) 507, 606
BsaJI CCNNGG 4 cut(s) 463, 817, 862, 1068
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 7 cut(s) 32, 284, 285, 406, 499, 568, 571
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 1 cut(s) 503
Bse8I GATNNNNATC 1 cut(s) 1137
BseBI CCWGG 2 cut(s) 899, 932
BseDI CCNNGG 4 cut(s) 463, 817, 862, 1068
BseGI GGATG 5 cut(s) 684, 927, 999, 1027, 1141
BseJI GATNNNNATC 1 cut(s) 1137
BseLI CCNNNNNNNGG 7 cut(s) 32, 284, 285, 406, 499, 568, 571
BseMII CTCAG 5 cut(s) 158, 453, 539, 565, 831
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 1 cut(s) 374
BseSI GKGCMC 2 cut(s) 279, 291
BseYI CCCAGC 2 cut(s) 284, 567
BshFI GGCC 2 cut(s) 289, 462
BshNI GGYRCC 3 cut(s) 274, 688, 813
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 5 cut(s) 202, 226, 401, 419, 632
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 7 cut(s) 32, 284, 285, 406, 499, 568, 571
BsmAI GTCTC 1 cut(s) 528
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 2 cut(s) 289, 462
BsoBI CYCGRG 1 cut(s) 335
Bsp120I GGGCCC 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 220, 279, 291
Bsp143I GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
BspANI GGCC 2 cut(s) 289, 462
BspCNI CTCAG 5 cut(s) 157, 452, 538, 564, 832
BspLI GGNNCC 6 cut(s) 276, 289, 290, 630, 690, 815
BspOI GCTAGC 1 cut(s) 201
BspPI GGATC 5 cut(s) 469, 527, 535, 1056, 1132
BspQI GCTCTTC 2 cut(s) 96, 208
BspT107I GGYRCC 3 cut(s) 274, 688, 813
BsrFI RCCGGY 1 cut(s) 225
BsrI ACTGG 1 cut(s) 503
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 4 cut(s) 463, 817, 862, 1068
BssMI GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
BssNI GRCGYC 2 cut(s) 507, 606
BssSI CACGAG 2 cut(s) 30, 137
BssT1I CCWWGG 2 cut(s) 817, 1068
Bst2BI CACGAG 2 cut(s) 30, 137
Bst2UI CCWGG 2 cut(s) 899, 932
Bst6I CTCTTC 3 cut(s) 96, 208, 755
BstACI GRCGYC 2 cut(s) 507, 606
BstC8I GCNNGC 4 cut(s) 199, 227, 460, 1098
BstDEI CTNAG 6 cut(s) 144, 439, 525, 551, 840, 1026
BstDSI CCRYGG 2 cut(s) 463, 862
BstF5I GGATG 5 cut(s) 684, 927, 999, 1027, 1141
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 8 cut(s) 231, 270, 326, 580, 666, 698, 730, 769
BstKTI GATC 8 cut(s) 429, 477, 535, 543, 717, 798, 1051, 1127
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
BstMWI GCNNNNNNNGC 8 cut(s) 231, 267, 274, 295, 323, 468, 702, 736
BstNI CCWGG 2 cut(s) 899, 932
BstSCI CCNGG 4 cut(s) 399, 631, 897, 930
BstSLI GKGCMC 2 cut(s) 279, 291
BstX2I RGATCY 1 cut(s) 426
BstYI RGATCY 1 cut(s) 426
BsuRI GGCC 2 cut(s) 289, 462
BtgI CCRYGG 2 cut(s) 463, 862
BtrI CACGTC 2 cut(s) 372, 834
BtsCI GGATG 5 cut(s) 684, 927, 999, 1027, 1141
BtsIMutI CAGTG 1 cut(s) 496
Cac8I GCNNGC 4 cut(s) 199, 227, 460, 1098
CaiI CAGNNNCTG 1 cut(s) 1096
CfoI GCGC 8 cut(s) 231, 270, 326, 580, 666, 698, 730, 769
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 6 cut(s) 35, 287, 288, 460, 629, 901
CseI GACGC 4 cut(s) 95, 323, 496, 614
Csp6I GTAC 4 cut(s) 814, 859, 961, 983
CviAII CATG 3 cut(s) 454, 845, 871
CviQI GTAC 4 cut(s) 814, 859, 961, 983
DdeI CTNAG 6 cut(s) 144, 439, 525, 551, 840, 1026
DpnI GATC 8 cut(s) 428, 476, 534, 542, 716, 797, 1050, 1126
DpnII GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
DraI TTTAAA 1 cut(s) 331
DriI GACNNNNNGTC 2 cut(s) 373, 835
Eam1104I CTCTTC 3 cut(s) 96, 208, 755
Eam1105I GACNNNNNGTC 2 cut(s) 373, 835
EarI CTCTTC 3 cut(s) 96, 208, 755
EciI GGCGGA 1 cut(s) 536
Ecl136II GAGCTC 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 817, 1068
Eco24I GRGCYC 2 cut(s) 220, 291
Eco47I GGWCC 3 cut(s) 35, 629, 901
Eco47III AGCGCT 1 cut(s) 325
Eco53kI GAGCTC 1 cut(s) 218
Eco57I CTGAAG 1 cut(s) 1061
Eco88I CYCGRG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 218
EcoO109I RGGNCCY 2 cut(s) 288, 901
EcoRII CCWGG 2 cut(s) 897, 930
EcoT14I CCWWGG 2 cut(s) 817, 1068
EcoT38I GRGCYC 2 cut(s) 220, 291
ErhI CCWWGG 2 cut(s) 817, 1068
FaeI CATG 3 cut(s) 457, 848, 874
FaiI YATR 7 cut(s) 179, 455, 602, 747, 755, 846, 872
FaqI GGGAC 1 cut(s) 515
FatI CATG 3 cut(s) 453, 844, 870
FauI CCCGC 2 cut(s) 230, 451
Fnu4HI GCNGC 6 cut(s) 209, 232, 469, 576, 622, 706
FokI GGATG 5 cut(s) 691, 914, 1006, 1034, 1128
FriOI GRGCYC 2 cut(s) 220, 291
Fsp4HI GCNGC 6 cut(s) 209, 232, 469, 576, 622, 706
FspBI CTAG 1 cut(s) 198
GlaI GCGC 8 cut(s) 230, 269, 325, 579, 665, 697, 729, 768
GluI GCNGC 6 cut(s) 209, 232, 469, 576, 622, 706
GsaI CCCAGC 2 cut(s) 288, 571
GsuI CTGGAG 1 cut(s) 969
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 2 cut(s) 289, 462
HapII CCGG 5 cut(s) 202, 226, 401, 419, 632
HgaI GACGC 4 cut(s) 95, 323, 496, 614
HhaI GCGC 8 cut(s) 231, 270, 326, 580, 666, 698, 730, 769
Hin1I GRCGYC 2 cut(s) 507, 606
Hin1II CATG 3 cut(s) 457, 848, 874
Hin6I GCGC 8 cut(s) 229, 268, 324, 578, 664, 696, 728, 767
HinP1I GCGC 8 cut(s) 229, 268, 324, 578, 664, 696, 728, 767
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 6 cut(s) 14, 142, 192, 410, 521, 1133
HpaII CCGG 5 cut(s) 202, 226, 401, 419, 632
HphI GGTGA 3 cut(s) 551, 769, 1049
Hpy166II GTNNAC 2 cut(s) 306, 559
Hpy188I TCNGA 7 cut(s) 13, 19, 117, 447, 520, 841, 1092
Hpy188III TCNNGA 2 cut(s) 139, 948
Hpy8I GTNNAC 2 cut(s) 306, 559
Hpy99I CGWCG 7 cut(s) 23, 260, 317, 376, 418, 512, 838
HpyAV CCTTC 7 cut(s) 136, 309, 546, 607, 924, 991, 1036
HpyCH4IV ACGT 2 cut(s) 371, 833
HpyF10VI GCNNNNNNNGC 8 cut(s) 231, 267, 274, 295, 323, 468, 702, 736
HpyF3I CTNAG 6 cut(s) 144, 439, 525, 551, 840, 1026
HpySE526I ACGT 2 cut(s) 371, 833
Hsp92I GRCGYC 2 cut(s) 507, 606
Hsp92II CATG 3 cut(s) 457, 848, 874
HspAI GCGC 8 cut(s) 229, 268, 324, 578, 664, 696, 728, 767
KpnI GGTACC 1 cut(s) 817
KroI GCCGGC 1 cut(s) 225
KroNI GCCGGC 1 cut(s) 227
Kzo9I GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
LguI GCTCTTC 2 cut(s) 96, 208
LmnI GCTCC 4 cut(s) 223, 421, 736, 1036
LweI GCATC 6 cut(s) 163, 748, 844, 1012, 1083, 1093
MaeI CTAG 1 cut(s) 198
MaeII ACGT 2 cut(s) 371, 833
MaeIII GTNAC 2 cut(s) 139, 479
MalI GATC 8 cut(s) 428, 476, 534, 542, 716, 797, 1050, 1126
MboI GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
MboII GAAGA 9 cut(s) 36, 113, 179, 182, 225, 421, 772, 793, 1046
MfeI CAATTG 1 cut(s) 986
MflI RGATCY 1 cut(s) 426
MhlI GDGCHC 3 cut(s) 220, 279, 291
MluCI AATT 2 cut(s) 972, 986
MlyI GAGTC 4 cut(s) 8, 136, 186, 530
MmeI TCCRAC 2 cut(s) 36, 42
MreI CGCCGGCG 1 cut(s) 225
MroNI GCCGGC 1 cut(s) 225
MseI TTAA 3 cut(s) 330, 353, 1002
MslI CAYNNNNRTG 2 cut(s) 744, 932
MspA1I CMGCKG 2 cut(s) 567, 1130
MspI CCGG 5 cut(s) 202, 226, 401, 419, 632
MspR9I CCNGG 4 cut(s) 401, 633, 899, 932
MunI CAATTG 1 cut(s) 986
MvaI CCWGG 2 cut(s) 899, 932
MwoI GCNNNNNNNGC 8 cut(s) 231, 267, 274, 295, 323, 468, 702, 736
NaeI GCCGGC 1 cut(s) 227
NciI CCSGG 2 cut(s) 401, 633
NdeII GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
NgoMIV GCCGGC 1 cut(s) 225
NheI GCTAGC 1 cut(s) 197
NlaIII CATG 3 cut(s) 457, 848, 874
NlaIV GGNNCC 6 cut(s) 276, 289, 290, 630, 690, 815
NmuCI GTSAC 2 cut(s) 139, 479
PciSI GCTCTTC 2 cut(s) 96, 208
PcsI WCGNNNNNNNCGW 1 cut(s) 516
PdiI GCCGGC 1 cut(s) 227
PfeI GAWTC 2 cut(s) 410, 1133
PflFI GACNNNGTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 631
PkrI GCNGC 6 cut(s) 210, 233, 470, 577, 623, 707
PleI GAGTC 4 cut(s) 8, 136, 186, 529
PpsI GAGTC 4 cut(s) 8, 136, 186, 529
PpuMI RGGWCCY 1 cut(s) 901
Psp124BI GAGCTC 1 cut(s) 220
Psp5II RGGWCCY 1 cut(s) 901
Psp6I CCWGG 2 cut(s) 897, 930
PspFI CCCAGC 2 cut(s) 284, 567
PspGI CCWGG 2 cut(s) 897, 930
PspN4I GGNNCC 6 cut(s) 276, 289, 290, 630, 690, 815
PspOMI GGGCCC 1 cut(s) 287
PspPI GGNCC 6 cut(s) 35, 287, 288, 460, 629, 901
PspPPI RGGWCCY 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 1096
PsuI RGATCY 1 cut(s) 426
PsyI GACNNNGTC 1 cut(s) 506
PvuII CAGCTG 1 cut(s) 1130
RsaI GTAC 4 cut(s) 815, 860, 962, 984
RsaNI GTAC 4 cut(s) 814, 859, 961, 983
RseI CAYNNNNRTG 2 cut(s) 744, 932
SacI GAGCTC 1 cut(s) 220
SapI GCTCTTC 2 cut(s) 96, 208
SaqAI TTAA 3 cut(s) 330, 353, 1002
SatI GCNGC 6 cut(s) 209, 232, 469, 576, 622, 706
Sau3AI GATC 8 cut(s) 426, 474, 532, 540, 714, 795, 1048, 1124
Sau96I GGNCC 6 cut(s) 35, 287, 288, 460, 629, 901
ScaI AGTACT 1 cut(s) 962
SchI GAGTC 4 cut(s) 8, 136, 186, 530
ScrFI CCNGG 4 cut(s) 401, 633, 899, 932
SduI GDGCHC 3 cut(s) 220, 279, 291
SfaNI GCATC 6 cut(s) 163, 748, 844, 1012, 1083, 1093
SgrAI CRCCGGYG 1 cut(s) 225
SinI GGWCC 3 cut(s) 35, 629, 901
SmiMI CAYNNNNRTG 2 cut(s) 744, 932
SmlI CTYRAG 3 cut(s) 152, 1074, 1112
SmoI CTYRAG 3 cut(s) 152, 1074, 1112
Sse9I AATT 2 cut(s) 972, 986
SspMI CTAG 1 cut(s) 198
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 4 cut(s) 399, 631, 897, 930
StyI CCWWGG 2 cut(s) 817, 1068
TaiI ACGT 2 cut(s) 374, 836
TaqI TCGA 1 cut(s) 539
TasI AATT 2 cut(s) 972, 986
TatI WGTACW 1 cut(s) 960
TauI GCSGC 6 cut(s) 211, 234, 471, 578, 624, 708
TfiI GAWTC 2 cut(s) 410, 1133
Tru1I TTAA 3 cut(s) 330, 353, 1002
Tru9I TTAA 3 cut(s) 330, 353, 1002
TscAI CASTG 1 cut(s) 503
TseFI GTSAC 2 cut(s) 139, 479
Tsp45I GTSAC 2 cut(s) 139, 479
TspDTI ATGAA 4 cut(s) 104, 114, 421, 1010
TspGWI ACGGA 2 cut(s) 467, 504
TspRI CASTG 1 cut(s) 503
Tth111I GACNNNGTC 1 cut(s) 506
Van91I CCANNNNNTGG 1 cut(s) 32
VpaK11BI GGWCC 3 cut(s) 35, 629, 901
XspI CTAG 1 cut(s) 198
ZrmI AGTACT 1 cut(s) 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.