RLG00000010621

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003321
Physical Location & Seq
Reverse (-)
3030135 .. 3038868
8734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010621

Sequence Viewer

Length: 366 bp
ATGGATCTGAATCACTGGAGTGAGAGATCAGAAAAATTTGCTGTTATTATGGTGAAGGGGAACATCTTCATTCTCGCCGGTCAGAGCAACATGGCCGGAGGAGGCGGCGTCAGCGGCAGCAAGTGGGACGGCAACATTCCTCCCGAGTGCACACCGACCCCGTCAATTCTCAGACTGAGTGCTCAACTCGTTTGGGAAGAAGCACGAGAGCCACTCCACGCTGACATTGATGTGGGCGAGGGCAAGACGTGTGGGGTTGGGCCAGGGATGGCTTTTGCCAACGAGGTCCTCCGAGCCAAAGGCTCGGCTATGGGAGTTTTGGGTCTGGTTCCTTGCGTTGTGGGCAGGACTAGAATCGGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

122

Amino Acids

12.7

Weight (kDa)

5.67

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 20 - 119 3.7e-35 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 105, 114
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 1 cut(s) 35
AcyI GRCGYC 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 248
AjiI CACGTC 1 cut(s) 249
AjnI CCWGG 1 cut(s) 262
AleI CACNNNNGTG 1 cut(s) 18
Alw21I GWGCWC 2 cut(s) 152, 184
Alw44I GTGCAC 1 cut(s) 148
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 93, 260
ApaLI GTGCAC 1 cut(s) 148
ApeKI GCWGC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 65
AspS9I GGNCC 2 cut(s) 260, 286
AsuHPI GGTGA 1 cut(s) 64
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 1 cut(s) 286
BaeGI GKGCMC 1 cut(s) 152
BauI CACGAG 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 152, 184
BbvI GCAGC 1 cut(s) 129
BccI CCATC 1 cut(s) 262
BceAI ACGGC 1 cut(s) 145
BciT130I CCWGG 1 cut(s) 264
BfaI CTAG 1 cut(s) 351
BisI GCNGC 3 cut(s) 106, 115, 118
BlsI GCNGC 3 cut(s) 107, 116, 119
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 286
BmeT110I CYCGRG 1 cut(s) 143
BmgBI CACGTC 1 cut(s) 249
BmgT120I GGNCC 2 cut(s) 260, 286
BmiI GGNNCC 1 cut(s) 330
BmrFI CCNGG 1 cut(s) 264
BplI GAGNNNNNCTC 2 cut(s) 198, 230
BpmI CTGGAG 1 cut(s) 37
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 93, 123
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 20
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 1 cut(s) 273
BseJI GATNNNNATC 1 cut(s) 9
BseMII CTCAG 2 cut(s) 167, 184
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 1 cut(s) 114
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 1 cut(s) 129
BshFI GGCC 2 cut(s) 95, 262
BsiHKAI GWGCWC 2 cut(s) 152, 184
BsiHKCI CYCGRG 1 cut(s) 143
BsiSI CCGG 2 cut(s) 78, 96
BslFI GGGAC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 140
BsnI GGCC 2 cut(s) 95, 262
BsoBI CYCGRG 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 152, 184
Bsp143I GATC 2 cut(s) 4, 26
BspACI CCGC 2 cut(s) 105, 114
BspANI GGCC 2 cut(s) 95, 262
BspCNI CTCAG 2 cut(s) 168, 183
BspLI GGNNCC 1 cut(s) 330
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 1 cut(s) 20
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 1 cut(s) 263
BssMI GATC 2 cut(s) 4, 26
BssNI GRCGYC 1 cut(s) 108
BssSI CACGAG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 264
BstACI GRCGYC 1 cut(s) 108
BstDEI CTNAG 2 cut(s) 170, 176
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 2 cut(s) 7, 29
BstMBI GATC 2 cut(s) 4, 26
BstMWI GCNNNNNNNGC 3 cut(s) 111, 114, 342
BstNI CCWGG 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 262
BstSLI GKGCMC 1 cut(s) 152
BstV1I GCAGC 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 2 cut(s) 95, 262
BtrI CACGTC 1 cut(s) 249
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 260, 286
CseI GACGC 1 cut(s) 97
CspCI CAANNNNNGTGG 2 cut(s) 232, 267
CviAII CATG 1 cut(s) 91
CviJI RGCY 7 cut(s) 95, 211, 262, 272, 296, 303, 308
CviKI_1 RGCY 7 cut(s) 95, 211, 262, 272, 296, 303, 308
DdeI CTNAG 2 cut(s) 170, 176
DpnI GATC 2 cut(s) 6, 28
DpnII GATC 2 cut(s) 4, 26
EaeI YGGCCR 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 262
FaeI CATG 1 cut(s) 94
FaiI YATR 3 cut(s) 50, 92, 311
FaqI GGGAC 1 cut(s) 140
FatI CATG 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 106, 115, 118
FokI GGATG 1 cut(s) 280
Fsp4HI GCNGC 3 cut(s) 106, 115, 118
FspBI CTAG 1 cut(s) 351
GluI GCNGC 3 cut(s) 106, 115, 118
GsuI CTGGAG 1 cut(s) 37
HaeIII GGCC 2 cut(s) 95, 262
HapII CCGG 2 cut(s) 78, 96
HgaI GACGC 1 cut(s) 97
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 1 cut(s) 94
HinfI GANTC 2 cut(s) 10, 354
HpaII CCGG 2 cut(s) 78, 96
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 5 cut(s) 9, 31, 84, 173, 293
Hpy188III TCNNGA 1 cut(s) 143
Hpy8I GTNNAC 1 cut(s) 150
HpyAV CCTTC 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 1 cut(s) 150
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 114, 342
HpyF3I CTNAG 2 cut(s) 170, 176
HpySE526I ACGT 1 cut(s) 248
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 1 cut(s) 94
Kzo9I GATC 2 cut(s) 4, 26
LpnPI CCDG 6 cut(s) 91, 109, 249, 276, 311, 331
Lsp1109I GCAGC 1 cut(s) 129
MaeI CTAG 1 cut(s) 351
MaeII ACGT 1 cut(s) 248
MalI GATC 2 cut(s) 6, 28
MboI GATC 2 cut(s) 4, 26
MboII GAAGA 2 cut(s) 58, 209
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 152, 184
MluCI AATT 2 cut(s) 35, 165
MnlI CCTC 6 cut(s) 92, 95, 150, 232, 277, 299
MroXI GAANNNNTTC 1 cut(s) 65
MslI CAYNNNNRTG 2 cut(s) 18, 230
MspA1I CMGCKG 1 cut(s) 114
MspI CCGG 2 cut(s) 78, 96
MspR9I CCNGG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 111, 114, 342
NdeII GATC 2 cut(s) 4, 26
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 1 cut(s) 330
NmeAIII GCCGAG 1 cut(s) 284
OliI CACNNNNGTG 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 65
PfeI GAWTC 2 cut(s) 10, 354
PflFI GACNNNGTC 1 cut(s) 160
PkrI GCNGC 3 cut(s) 107, 116, 119
PpuMI RGGWCCY 1 cut(s) 286
Psp5II RGGWCCY 1 cut(s) 286
Psp6I CCWGG 1 cut(s) 262
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 330
PspPI GGNCC 2 cut(s) 260, 286
PspPPI RGGWCCY 1 cut(s) 286
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 160
RseI CAYNNNNRTG 2 cut(s) 18, 230
SatI GCNGC 3 cut(s) 106, 115, 118
Sau3AI GATC 2 cut(s) 4, 26
Sau96I GGNCC 2 cut(s) 260, 286
ScrFI CCNGG 1 cut(s) 264
SduI GDGCHC 2 cut(s) 152, 184
SetI ASST 2 cut(s) 251, 288
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 18, 230
Sse9I AATT 2 cut(s) 35, 165
SsiI CCGC 2 cut(s) 105, 114
SspMI CTAG 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 262
TaiI ACGT 1 cut(s) 251
TasI AATT 2 cut(s) 35, 165
TauI GCSGC 2 cut(s) 108, 117
TfiI GAWTC 2 cut(s) 10, 354
TscAI CASTG 1 cut(s) 20
TseI GCWGC 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 58
TspRI CASTG 1 cut(s) 20
Tth111I GACNNNGTC 1 cut(s) 160
VneI GTGCAC 1 cut(s) 148
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 1 cut(s) 35
XmnI GAANNNNTTC 1 cut(s) 65
XspI CTAG 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.