RchiOBHm_Chr6g0255411

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003321
Physical Location & Seq
Forward (+)
10740561 .. 10743015
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22907

Sequence Viewer

Length: 1341 bp
ATGGACAAAGGCGCCAGAGGGTGCCAGCATAGACAACAAAATATTATTCACTTCAATCATTCAACTCTATCCTTTCACTGCTTGTTCCCTCTACAAAAGGGCATCACCTTCTTCAACATTTCATTTCCGTCTCATTTCTTCTTAAACTCTTCCACATCCCTCTCCTCAACCATGAACTCCGATCTCGACCCAACTCGTAATCCATTCTCTTCTCGTTTTCCTCGTTCAAGGCTTATGCGACTTTGTCTGGATGATATGAATGCCTCTGCTCAAAGGCGGGCTCTGTTAATGCGTGGCATGTTTACTCTCTTAGCTCAATATCTCGACGACGACGACGACGAAGACGAAGAGGAGGAGGACGAGGATCCCCAAGAACAAGCCGGAGAACAAGACCCACTCGCTAACGGGAAGCGGCGACGCTCAACTACTCGAAACCCAAACGACGACGTTCCGCGCAAGGCCCACGCTGGTCCCCACTCGCCTTCGTTGACAAGCGACGCGGAGAGCCCATTTAAACCAGAGAACTCGCTCATTCGAAGAACAAAACCCGACACGTCGTTTATCGTCCACCCGAAAAACCCGGAAATGGAGTCGCCGGAGCAGATCTTCATTCTTTCGGGGCAGAGCAACATGGCTGGTCGGGGCGGCGTGATCCGGGACCACAGCCACCACAACCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACTCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGGGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGCACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGGAGAGATCAAAGGGTTGCTGTGGTACCAGGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGAAGCGTACCACGAGAACATGGTTAGGCTTATTGAGAATGTGCGCCGGGACCTTGCTTTGCCTTCACTTCCAGTAATCCAGGTGGCTATTTGTTCCGGTGATGAGAAGTACTTGGAGAAAATTAGGGAGATTCAATTGGGGATGAAGGTTGAGAATGTTGTGTGTGTGGATGCTAAGGGATTGGAGCTGAAAGATGATCATCTTCACCTGACCACCGAGGCTCAAGTTCAGTTGGGTCGGATGCTGGCTGATGCTTACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

446

Amino Acids

50.01

Weight (kDa)

6.05

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 200 - 434 9.5e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 999
AccB1I GGYRCC 4 cut(s) 11, 21, 874, 999
AccII CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
AclWI GGATC 5 cut(s) 359, 372, 646, 721, 1318
AcyI GRCGYC 2 cut(s) 12, 693
AfaI GTAC 4 cut(s) 932, 1001, 1046, 1148
AfiI CCNNNNNNNGG 4 cut(s) 586, 685, 754, 757
AflIII ACRYGT 2 cut(s) 552, 1017
AgsI TTSAA 6 cut(s) 55, 63, 115, 228, 1040, 1172
AhdI GACNNNNNGTC 1 cut(s) 1021
AjiI CACGTC 2 cut(s) 555, 1020
AjnI CCWGG 2 cut(s) 1002, 1116
AluBI AGCT 3 cut(s) 314, 1225, 1316
AluI AGCT 3 cut(s) 314, 1225, 1316
Alw26I GTCTC 2 cut(s) 135, 1029
AlwI GGATC 5 cut(s) 359, 372, 646, 721, 1318
AoxI GGCC 1 cut(s) 459
Asp718I GGTACC 1 cut(s) 999
AspLEI GCGC 8 cut(s) 14, 456, 766, 852, 884, 916, 955, 1083
AspS9I GGNCC 5 cut(s) 460, 470, 658, 815, 1087
AsuC2I CCSGG 4 cut(s) 581, 656, 819, 1085
AsuHPI GGTGA 6 cut(s) 97, 737, 853, 955, 1148, 1235
AsuII TTCGAA 1 cut(s) 535
AvaII GGWCC 4 cut(s) 470, 658, 815, 1087
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 4 cut(s) 11, 21, 874, 999
BanII GRGCYC 2 cut(s) 283, 509
BauI CACGAG 1 cut(s) 1049
BbsI GAAGAC 1 cut(s) 348
BccI CCATC 1 cut(s) 1332
BceAI ACGGC 3 cut(s) 706, 862, 912
BciT130I CCWGG 2 cut(s) 1004, 1118
BclI TGATCA 1 cut(s) 1234
BcnI CCSGG 4 cut(s) 581, 656, 819, 1085
BcoDI GTCTC 2 cut(s) 135, 1029
BfoI RGCGCY 1 cut(s) 15
BglII AGATCT 1 cut(s) 603
BisI GCNGC 4 cut(s) 413, 646, 762, 892
BlpI GCTNAGC 1 cut(s) 711
BlsI GCNGC 4 cut(s) 414, 647, 763, 893
BmcAI AGTACT 1 cut(s) 1148
Bme1390I CCNGG 6 cut(s) 581, 656, 819, 1004, 1085, 1118
Bme18I GGWCC 4 cut(s) 470, 658, 815, 1087
BmeRI GACNNNNNGTC 1 cut(s) 1021
BmgBI CACGTC 2 cut(s) 555, 1020
BmgT120I GGNCC 5 cut(s) 460, 470, 658, 815, 1087
BmiI GGNNCC 9 cut(s) 13, 23, 366, 472, 659, 816, 876, 1001, 1088
BmrFI CCNGG 6 cut(s) 581, 656, 819, 1004, 1085, 1118
BmrI ACTGGG 2 cut(s) 694, 763
BmsI GCATC 5 cut(s) 111, 934, 1198, 1269, 1279
BmuI ACTGGG 2 cut(s) 694, 763
BpiI GAAGAC 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 1212
Bpu1102I GCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 535
BpuEI CTTGAG 2 cut(s) 1245, 1283
BpuMI CCSGG 4 cut(s) 581, 656, 819, 1085
BsaBI GATNNNNATC 1 cut(s) 1236
BsaHI GRCGYC 2 cut(s) 12, 693
BsaJI CCNNGG 2 cut(s) 1003, 1254
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 4 cut(s) 776, 806, 914, 944
Bsc4I CCNNNNNNNGG 4 cut(s) 586, 685, 754, 757
Bse1I ACTGG 3 cut(s) 689, 758, 1109
Bse8I GATNNNNATC 1 cut(s) 1236
BseBI CCWGG 2 cut(s) 1004, 1118
BseDI CCNNGG 2 cut(s) 1003, 1254
BseGI GGATG 6 cut(s) 155, 256, 1185, 1213, 1284, 1327
BseJI GATNNNNATC 1 cut(s) 1236
BseLI CCNNNNNNNGG 4 cut(s) 586, 685, 754, 757
BseMII CTCAG 2 cut(s) 725, 1040
BseNI ACTGG 3 cut(s) 689, 758, 1109
BseRI GAGGAG 3 cut(s) 154, 365, 368
Bsh1236I CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
BshFI GGCC 1 cut(s) 461
BshNI GGYRCC 4 cut(s) 11, 21, 874, 999
BsiSI CCGG 7 cut(s) 381, 581, 596, 655, 818, 1084, 1134
BslFI GGGAC 4 cut(s) 456, 671, 701, 1100
BslI CCNNNNNNNGG 4 cut(s) 586, 685, 754, 757
BsmAI GTCTC 2 cut(s) 135, 1029
BsmBI CGTCTC 2 cut(s) 135, 1029
BsmFI GGGAC 4 cut(s) 456, 671, 701, 1100
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 1 cut(s) 461
Bsp119I TTCGAA 1 cut(s) 535
Bsp1286I GDGCHC 2 cut(s) 283, 509
Bsp143I GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
Bsp1720I GCTNAGC 1 cut(s) 711
BspANI GGCC 1 cut(s) 461
BspCNI CTCAG 2 cut(s) 724, 1039
BspFNI CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
BspLI GGNNCC 9 cut(s) 13, 23, 366, 472, 659, 816, 876, 1001, 1088
BspPI GGATC 5 cut(s) 359, 372, 646, 721, 1318
BspT104I TTCGAA 1 cut(s) 535
BspT107I GGYRCC 4 cut(s) 11, 21, 874, 999
BsrI ACTGG 3 cut(s) 689, 758, 1109
BssECI CCNNGG 2 cut(s) 1003, 1254
BssMI GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
BssNI GRCGYC 2 cut(s) 12, 693
BssSI CACGAG 1 cut(s) 1049
Bst2BI CACGAG 1 cut(s) 1049
Bst2UI CCWGG 2 cut(s) 1004, 1118
Bst6I CTCTTC 4 cut(s) 154, 214, 342, 941
BstACI GRCGYC 2 cut(s) 12, 693
BstBI TTCGAA 1 cut(s) 535
BstC8I GCNNGC 3 cut(s) 26, 279, 1284
BstDEI CTNAG 4 cut(s) 310, 711, 1026, 1212
BstF5I GGATG 6 cut(s) 155, 256, 1185, 1213, 1284, 1327
BstFNI CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 8 cut(s) 14, 456, 766, 852, 884, 916, 955, 1083
BstKTI GATC 9 cut(s) 184, 367, 606, 654, 729, 903, 984, 1237, 1313
BstMAI GTCTC 2 cut(s) 135, 1029
BstMBI GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
BstMWI GCNNNNNNNGC 2 cut(s) 888, 922
BstNI CCWGG 2 cut(s) 1004, 1118
BstNSI RCATGY 1 cut(s) 301
BstSCI CCNGG 6 cut(s) 579, 654, 817, 1002, 1083, 1116
BstUI CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 364, 603
BstYI RGATCY 2 cut(s) 364, 603
BsuRI GGCC 1 cut(s) 461
BtrI CACGTC 2 cut(s) 555, 1020
BtsCI GGATG 6 cut(s) 155, 256, 1185, 1213, 1284, 1327
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 682, 751
Cac8I GCNNGC 3 cut(s) 26, 279, 1284
CfoI GCGC 8 cut(s) 14, 456, 766, 852, 884, 916, 955, 1083
Cfr13I GGNCC 5 cut(s) 460, 470, 658, 815, 1087
CseI GACGC 3 cut(s) 426, 506, 682
Csp6I GTAC 4 cut(s) 931, 1000, 1045, 1147
CviAII CATG 6 cut(s) 172, 298, 631, 940, 1031, 1057
CviQI GTAC 4 cut(s) 931, 1000, 1045, 1147
DdeI CTNAG 4 cut(s) 310, 711, 1026, 1212
DinI GGCGCC 1 cut(s) 13
DpnI GATC 9 cut(s) 183, 366, 605, 653, 728, 902, 983, 1236, 1312
DpnII GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
DraI TTTAAA 1 cut(s) 514
DriI GACNNNNNGTC 1 cut(s) 1021
Eam1104I CTCTTC 4 cut(s) 154, 214, 342, 941
Eam1105I GACNNNNNGTC 1 cut(s) 1021
EarI CTCTTC 4 cut(s) 154, 214, 342, 941
EciI GGCGGA 1 cut(s) 722
Eco24I GRGCYC 2 cut(s) 283, 509
Eco47I GGWCC 4 cut(s) 470, 658, 815, 1087
EcoO109I RGGNCCY 1 cut(s) 1087
EcoRII CCWGG 2 cut(s) 1002, 1116
EcoT38I GRGCYC 2 cut(s) 283, 509
EgeI GGCGCC 1 cut(s) 13
EheI GGCGCC 1 cut(s) 13
Esp3I CGTCTC 2 cut(s) 135, 1029
FaeI CATG 6 cut(s) 175, 301, 634, 943, 1034, 1060
FaiI YATR 9 cut(s) 30, 173, 236, 257, 299, 632, 941, 1032, 1058
FaqI GGGAC 4 cut(s) 456, 671, 701, 1100
FatI CATG 6 cut(s) 171, 297, 630, 939, 1030, 1056
FauI CCCGC 1 cut(s) 270
FbaI TGATCA 1 cut(s) 1234
Fnu4HI GCNGC 4 cut(s) 413, 646, 762, 892
FokI GGATG 6 cut(s) 142, 263, 1192, 1220, 1291, 1314
FriOI GRGCYC 2 cut(s) 283, 509
Fsp4HI GCNGC 4 cut(s) 413, 646, 762, 892
GlaI GCGC 8 cut(s) 13, 455, 765, 851, 883, 915, 954, 1082
GluI GCNGC 4 cut(s) 413, 646, 762, 892
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 1 cut(s) 461
HapII CCGG 7 cut(s) 381, 581, 596, 655, 818, 1084, 1134
HgaI GACGC 3 cut(s) 426, 506, 682
HhaI GCGC 8 cut(s) 14, 456, 766, 852, 884, 916, 955, 1083
Hin1I GRCGYC 2 cut(s) 12, 693
Hin1II CATG 6 cut(s) 175, 301, 634, 943, 1034, 1060
Hin6I GCGC 8 cut(s) 12, 454, 764, 850, 882, 914, 953, 1081
HinP1I GCGC 8 cut(s) 12, 454, 764, 850, 882, 914, 953, 1081
HincII GTYRAC 1 cut(s) 489
HindII GTYRAC 1 cut(s) 489
HinfI GANTC 3 cut(s) 590, 791, 1168
HpaII CCGG 7 cut(s) 381, 581, 596, 655, 818, 1084, 1134
HphI GGTGA 6 cut(s) 97, 737, 853, 955, 1148, 1235
Hpy166II GTNNAC 3 cut(s) 303, 489, 568
Hpy188I TCNGA 3 cut(s) 181, 706, 1278
Hpy188III TCNNGA 3 cut(s) 185, 248, 323
Hpy8I GTNNAC 3 cut(s) 303, 489, 568
HpyAV CCTTC 6 cut(s) 118, 492, 732, 793, 1110, 1177
HpyCH4IV ACGT 3 cut(s) 447, 554, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 888, 922
HpyF3I CTNAG 4 cut(s) 310, 711, 1026, 1212
HpySE526I ACGT 3 cut(s) 447, 554, 1019
Hsp92I GRCGYC 2 cut(s) 12, 693
Hsp92II CATG 6 cut(s) 175, 301, 634, 943, 1034, 1060
HspAI GCGC 8 cut(s) 12, 454, 764, 850, 882, 914, 953, 1081
KasI GGCGCC 1 cut(s) 11
KpnI GGTACC 1 cut(s) 1003
Ksp22I TGATCA 1 cut(s) 1234
Kzo9I GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
LmnI GCTCC 3 cut(s) 598, 922, 1222
LweI GCATC 5 cut(s) 111, 934, 1198, 1269, 1279
MaeII ACGT 3 cut(s) 447, 554, 1019
MalI GATC 9 cut(s) 183, 366, 605, 653, 728, 902, 983, 1236, 1312
MboI GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
MfeI CAATTG 1 cut(s) 1172
MflI RGATCY 2 cut(s) 364, 603
MhlI GDGCHC 2 cut(s) 283, 509
MluCI AATT 3 cut(s) 1158, 1172, 1318
Mly113I GGCGCC 1 cut(s) 12
MlyI GAGTC 2 cut(s) 599, 785
MmeI TCCRAC 2 cut(s) 684, 1256
MseI TTAA 3 cut(s) 143, 287, 513
MspI CCGG 7 cut(s) 381, 581, 596, 655, 818, 1084, 1134
MspR9I CCNGG 6 cut(s) 581, 656, 819, 1004, 1085, 1118
MunI CAATTG 1 cut(s) 1172
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 1004, 1118
MvnI CGCG 8 cut(s) 454, 500, 766, 839, 852, 884, 916, 955
MwoI GCNNNNNNNGC 2 cut(s) 888, 922
NarI GGCGCC 1 cut(s) 12
NciI CCSGG 4 cut(s) 581, 656, 819, 1085
NdeII GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
NlaIII CATG 6 cut(s) 175, 301, 634, 943, 1034, 1060
NlaIV GGNNCC 9 cut(s) 13, 23, 366, 472, 659, 816, 876, 1001, 1088
NspI RCATGY 1 cut(s) 301
NspV TTCGAA 1 cut(s) 535
PcsI WCGNNNNNNNCGW 6 cut(s) 220, 330, 333, 336, 342, 786
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 1168
PflFI GACNNNGTC 2 cut(s) 243, 692
PfoI TCCNGGA 2 cut(s) 654, 817
PkrI GCNGC 4 cut(s) 414, 647, 763, 893
PleI GAGTC 2 cut(s) 598, 785
PluTI GGCGCC 1 cut(s) 15
PpsI GAGTC 2 cut(s) 598, 785
PpuMI RGGWCCY 1 cut(s) 1087
Psp5II RGGWCCY 1 cut(s) 1087
Psp6I CCWGG 2 cut(s) 1002, 1116
PspGI CCWGG 2 cut(s) 1002, 1116
PspN4I GGNNCC 9 cut(s) 13, 23, 366, 472, 659, 816, 876, 1001, 1088
PspPI GGNCC 5 cut(s) 460, 470, 658, 815, 1087
PspPPI RGGWCCY 1 cut(s) 1087
PsuI RGATCY 2 cut(s) 364, 603
PsyI GACNNNGTC 2 cut(s) 243, 692
RsaI GTAC 4 cut(s) 932, 1001, 1046, 1148
RsaNI GTAC 4 cut(s) 931, 1000, 1045, 1147
SaqAI TTAA 3 cut(s) 143, 287, 513
SatI GCNGC 4 cut(s) 413, 646, 762, 892
Sau3AI GATC 9 cut(s) 181, 364, 603, 651, 726, 900, 981, 1234, 1310
Sau96I GGNCC 5 cut(s) 460, 470, 658, 815, 1087
ScaI AGTACT 1 cut(s) 1148
SchI GAGTC 2 cut(s) 599, 785
ScrFI CCNGG 6 cut(s) 581, 656, 819, 1004, 1085, 1118
SduI GDGCHC 2 cut(s) 283, 509
SfaNI GCATC 5 cut(s) 111, 934, 1198, 1269, 1279
SfoI GGCGCC 1 cut(s) 13
SfuI TTCGAA 1 cut(s) 535
SinI GGWCC 4 cut(s) 470, 658, 815, 1087
SmlI CTYRAG 2 cut(s) 1260, 1298
SmoI CTYRAG 2 cut(s) 1260, 1298
Sse9I AATT 3 cut(s) 1158, 1172, 1318
SspDI GGCGCC 1 cut(s) 11
SspI AATATT 1 cut(s) 43
StyD4I CCNGG 6 cut(s) 579, 654, 817, 1002, 1083, 1116
TaiI ACGT 3 cut(s) 450, 557, 1022
TaqI TCGA 6 cut(s) 186, 324, 430, 535, 725, 789
TasI AATT 3 cut(s) 1158, 1172, 1318
TatI WGTACW 1 cut(s) 1146
TauI GCSGC 4 cut(s) 415, 648, 764, 894
TfiI GAWTC 1 cut(s) 1168
Tru1I TTAA 3 cut(s) 143, 287, 513
Tru9I TTAA 3 cut(s) 143, 287, 513
TscAI CASTG 3 cut(s) 83, 689, 758
TspDTI ATGAA 5 cut(s) 111, 188, 272, 598, 1196
TspGWI ACGGA 2 cut(s) 117, 690
TspRI CASTG 3 cut(s) 83, 689, 758
Tth111I GACNNNGTC 2 cut(s) 243, 692
VpaK11BI GGWCC 4 cut(s) 470, 658, 815, 1087
XceI RCATGY 1 cut(s) 301
ZrmI AGTACT 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.