Rh6DG065000

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003321
Physical Location & Seq
Forward (+)
6660105 .. 6661051
947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG065000.1

Sequence Viewer

Length: 756 bp
ATGGAGTCGCCGGAGCAGATCTTCATTCTCTCGGGGCAGAGCAACATGGCGGGTCGGGGCGGCGTGATCCGCGACCACAGCCACGACCATCACCAGCACTGGGACGGCGTCGTTCCGTCGGAGGCTCAGCCCGACCCTTCGATCCTCCGCCTCAACGCTCACCTCCACTGGGAGGCGGCGCGTGAGCCTCTCCACGCCGACATCGACGCCAAGAAGGTGTGCGGTTTGGGTCCGGGAATGTCGTTCGCCAACGCGGTGAGGGAGCGCGTGGAGGGGAAGATTGGGCTGGTGCCGTGCGCGGTTGGCGGGACGGCGATCAAGGAGTGGGCGCGTGGGGAGCATCTGTACGAGAACATGGTGAAGAGGGCGCGTGAGAGCGTGAAGAACGGGGCAGAGATCAAAGGGTTGCTGTGGTACCAAGGGGAGAGCGACACGTCGTCTCAGCATGATGTTGATGCGTACCACGAGAACATGGTTAGGCCCATTGAGAATGTGCGCCAGGACCTTGCTTTGCCTTCACTTCCAATAATCCAGGTGGCTATTTGCTCCGGAGATGAGAAGTACTTGGAGAAAATTAGGGAGATTCAATTGGGGATGAAGGTTGAGAATGTTGTGAGTGTGGATGCTAAGGGATTGGAGCTGAAAGATGATCATCTTCACCTGACCACTGAGGCTCAAGTTCAGTTGGGTCGGATGCTGGCTGATGCTTACCTCAAGCATTTTGGATCAGCTAATTCCCATCATCCTGCCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

251

Amino Acids

27.78

Weight (kDa)

5.86

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 5 - 239 1.7e-94 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 2 cut(s) 289, 414
AccII CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
AccIII TCCGGA 1 cut(s) 548
AciI CCGC 9 cut(s) 50, 60, 70, 148, 176, 222, 254, 299, 306
AclWI GGATC 3 cut(s) 61, 136, 733
AcyI GRCGYC 2 cut(s) 108, 207
AfaI GTAC 4 cut(s) 347, 416, 461, 563
AfiI CCNNNNNNNGG 3 cut(s) 100, 169, 172
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 1 cut(s) 587
AhdI GACNNNNNGTC 1 cut(s) 436
AjiI CACGTC 1 cut(s) 435
AjnI CCWGG 2 cut(s) 498, 531
AluBI AGCT 2 cut(s) 640, 731
AluI AGCT 2 cut(s) 640, 731
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 3 cut(s) 61, 136, 733
Ama87I CYCGRG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 548
AoxI GGCC 1 cut(s) 479
Asp718I GGTACC 1 cut(s) 414
AspLEI GCGC 6 cut(s) 181, 267, 299, 331, 370, 498
AspS9I GGNCC 3 cut(s) 230, 480, 502
AsuC2I CCSGG 1 cut(s) 234
AsuHPI GGTGA 5 cut(s) 83, 152, 268, 370, 650
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 2 cut(s) 230, 502
BanI GGYRCC 2 cut(s) 289, 414
BauI CACGAG 1 cut(s) 464
BccI CCATC 2 cut(s) 96, 747
BceAI ACGGC 3 cut(s) 121, 277, 327
BciT130I CCWGG 2 cut(s) 500, 533
BclI TGATCA 1 cut(s) 649
BcnI CCSGG 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 444
BglII AGATCT 1 cut(s) 18
BisI GCNGC 2 cut(s) 61, 177
BlpI GCTNAGC 1 cut(s) 126
BlsI GCNGC 2 cut(s) 62, 178
BmcAI AGTACT 1 cut(s) 563
Bme1390I CCNGG 3 cut(s) 234, 500, 533
Bme18I GGWCC 2 cut(s) 230, 502
BmeRI GACNNNNNGTC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 31
BmgBI CACGTC 1 cut(s) 435
BmgT120I GGNCC 3 cut(s) 230, 480, 502
BmiI GGNNCC 3 cut(s) 231, 291, 416
BmrFI CCNGG 3 cut(s) 234, 500, 533
BmrI ACTGGG 2 cut(s) 109, 178
BmsI GCATC 5 cut(s) 349, 445, 613, 684, 694
BmuI ACTGGG 2 cut(s) 109, 178
Bpu10I CCTNAGC 1 cut(s) 627
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 660, 698
BpuMI CCSGG 1 cut(s) 234
BsaBI GATNNNNATC 1 cut(s) 651
BsaHI GRCGYC 2 cut(s) 108, 207
BsaJI CCNNGG 1 cut(s) 418
BsaWI WCCGGW 1 cut(s) 548
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 169, 172
Bse1I ACTGG 2 cut(s) 104, 173
Bse8I GATNNNNATC 1 cut(s) 651
BseAI TCCGGA 1 cut(s) 548
BseBI CCWGG 2 cut(s) 500, 533
BseDI CCNNGG 1 cut(s) 418
BseGI GGATG 4 cut(s) 600, 628, 699, 742
BseJI GATNNNNATC 1 cut(s) 651
BseLI CCNNNNNNNGG 3 cut(s) 100, 169, 172
BseMII CTCAG 3 cut(s) 140, 455, 660
BseNI ACTGG 2 cut(s) 104, 173
Bsh1236I CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
BshFI GGCC 1 cut(s) 481
BshNI GGYRCC 2 cut(s) 289, 414
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 3 cut(s) 11, 233, 549
BslFI GGGAC 2 cut(s) 116, 322
BslI CCNNNNNNNGG 3 cut(s) 100, 169, 172
BsmAI GTCTC 1 cut(s) 444
BsmBI CGTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 116, 322
BsnI GGCC 1 cut(s) 481
BsoBI CYCGRG 1 cut(s) 31
Bsp13I TCCGGA 1 cut(s) 548
Bsp143I GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 9 cut(s) 50, 60, 70, 148, 176, 222, 254, 299, 306
BspANI GGCC 1 cut(s) 481
BspCNI CTCAG 3 cut(s) 139, 454, 661
BspEI TCCGGA 1 cut(s) 548
BspFNI CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
BspLI GGNNCC 3 cut(s) 231, 291, 416
BspPI GGATC 3 cut(s) 61, 136, 733
BspT107I GGYRCC 2 cut(s) 289, 414
BsrI ACTGG 2 cut(s) 104, 173
BssECI CCNNGG 1 cut(s) 418
BssMI GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
BssNI GRCGYC 2 cut(s) 108, 207
BssSI CACGAG 1 cut(s) 464
BssT1I CCWWGG 1 cut(s) 418
Bst2BI CACGAG 1 cut(s) 464
Bst2UI CCWGG 2 cut(s) 500, 533
Bst6I CTCTTC 1 cut(s) 356
BstACI GRCGYC 2 cut(s) 108, 207
BstC8I GCNNGC 1 cut(s) 699
BstDEI CTNAG 4 cut(s) 126, 441, 627, 669
BstF5I GGATG 4 cut(s) 600, 628, 699, 742
BstFNI CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
BstHHI GCGC 6 cut(s) 181, 267, 299, 331, 370, 498
BstKTI GATC 7 cut(s) 21, 69, 144, 318, 399, 652, 728
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
BstMWI GCNNNNNNNGC 4 cut(s) 69, 78, 303, 337
BstNI CCWGG 2 cut(s) 500, 533
BstSCI CCNGG 3 cut(s) 232, 498, 531
BstUI CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
BstX2I RGATCY 1 cut(s) 18
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 1 cut(s) 481
BtrI CACGTC 1 cut(s) 435
BtsCI GGATG 4 cut(s) 600, 628, 699, 742
BtsIMutI CAGTG 3 cut(s) 97, 166, 666
Cac8I GCNNGC 1 cut(s) 699
CfoI GCGC 6 cut(s) 181, 267, 299, 331, 370, 498
Cfr13I GGNCC 3 cut(s) 230, 480, 502
CseI GACGC 2 cut(s) 97, 215
Csp6I GTAC 4 cut(s) 346, 415, 460, 562
CviAII CATG 4 cut(s) 46, 355, 446, 472
CviQI GTAC 4 cut(s) 346, 415, 460, 562
DdeI CTNAG 4 cut(s) 126, 441, 627, 669
DpnI GATC 7 cut(s) 20, 68, 143, 317, 398, 651, 727
DpnII GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
DriI GACNNNNNGTC 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 356
Eam1105I GACNNNNNGTC 1 cut(s) 436
EarI CTCTTC 1 cut(s) 356
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 230, 502
Eco88I CYCGRG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 498, 531
EcoT14I CCWWGG 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 444
FaeI CATG 4 cut(s) 49, 358, 449, 475
FaiI YATR 4 cut(s) 47, 356, 447, 473
FaqI GGGAC 2 cut(s) 116, 322
FatI CATG 4 cut(s) 45, 354, 445, 471
FauI CCCGC 2 cut(s) 43, 299
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 2 cut(s) 61, 177
FokI GGATG 4 cut(s) 607, 635, 706, 729
Fsp4HI GCNGC 2 cut(s) 61, 177
GlaI GCGC 6 cut(s) 180, 266, 298, 330, 369, 497
GluI GCNGC 2 cut(s) 61, 177
HaeIII GGCC 1 cut(s) 481
HapII CCGG 3 cut(s) 11, 233, 549
HgaI GACGC 2 cut(s) 97, 215
HhaI GCGC 6 cut(s) 181, 267, 299, 331, 370, 498
Hin1I GRCGYC 2 cut(s) 108, 207
Hin1II CATG 4 cut(s) 49, 358, 449, 475
Hin6I GCGC 6 cut(s) 179, 265, 297, 329, 368, 496
HinP1I GCGC 6 cut(s) 179, 265, 297, 329, 368, 496
HinfI GANTC 2 cut(s) 5, 583
HpaII CCGG 3 cut(s) 11, 233, 549
HphI GGTGA 5 cut(s) 83, 152, 268, 370, 650
Hpy188I TCNGA 2 cut(s) 121, 693
Hpy188III TCNNGA 1 cut(s) 549
Hpy99I CGWCG 4 cut(s) 113, 121, 209, 439
HpyAV CCTTC 4 cut(s) 147, 208, 525, 592
HpyCH4IV ACGT 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 78, 303, 337
HpyF3I CTNAG 4 cut(s) 126, 441, 627, 669
HpySE526I ACGT 1 cut(s) 434
Hsp92I GRCGYC 2 cut(s) 108, 207
Hsp92II CATG 4 cut(s) 49, 358, 449, 475
HspAI GCGC 6 cut(s) 179, 265, 297, 329, 368, 496
Kpn2I TCCGGA 1 cut(s) 548
KpnI GGTACC 1 cut(s) 418
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
LmnI GCTCC 5 cut(s) 13, 262, 337, 551, 637
LweI GCATC 5 cut(s) 349, 445, 613, 684, 694
MaeII ACGT 1 cut(s) 434
MalI GATC 7 cut(s) 20, 68, 143, 317, 398, 651, 727
MboI GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
MboII GAAGA 5 cut(s) 13, 289, 373, 394, 647
MfeI CAATTG 1 cut(s) 587
MflI RGATCY 1 cut(s) 18
MluCI AATT 3 cut(s) 573, 587, 733
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 99, 671
MroI TCCGGA 1 cut(s) 548
MspI CCGG 3 cut(s) 11, 233, 549
MspR9I CCNGG 3 cut(s) 234, 500, 533
MunI CAATTG 1 cut(s) 587
MvaI CCWGG 2 cut(s) 500, 533
MvnI CGCG 7 cut(s) 72, 181, 254, 267, 299, 331, 370
MwoI GCNNNNNNNGC 4 cut(s) 69, 78, 303, 337
NciI CCSGG 1 cut(s) 234
NdeII GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
NlaIII CATG 4 cut(s) 49, 358, 449, 475
NlaIV GGNNCC 3 cut(s) 231, 291, 416
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PfeI GAWTC 1 cut(s) 583
PflFI GACNNNGTC 1 cut(s) 107
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 2 cut(s) 62, 178
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 498, 531
PspGI CCWGG 2 cut(s) 498, 531
PspN4I GGNNCC 3 cut(s) 231, 291, 416
PspPI GGNCC 3 cut(s) 230, 480, 502
PspPPI RGGWCCY 1 cut(s) 502
PsuI RGATCY 1 cut(s) 18
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 4 cut(s) 347, 416, 461, 563
RsaNI GTAC 4 cut(s) 346, 415, 460, 562
SatI GCNGC 2 cut(s) 61, 177
Sau3AI GATC 7 cut(s) 18, 66, 141, 315, 396, 649, 725
Sau96I GGNCC 3 cut(s) 230, 480, 502
ScaI AGTACT 1 cut(s) 563
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 3 cut(s) 234, 500, 533
SfaNI GCATC 5 cut(s) 349, 445, 613, 684, 694
SinI GGWCC 2 cut(s) 230, 502
SmlI CTYRAG 2 cut(s) 675, 713
SmoI CTYRAG 2 cut(s) 675, 713
Sse9I AATT 3 cut(s) 573, 587, 733
SsiI CCGC 9 cut(s) 50, 60, 70, 148, 176, 222, 254, 299, 306
StyD4I CCNGG 3 cut(s) 232, 498, 531
StyI CCWWGG 1 cut(s) 418
TaiI ACGT 1 cut(s) 437
TaqI TCGA 2 cut(s) 140, 204
TasI AATT 3 cut(s) 573, 587, 733
TatI WGTACW 1 cut(s) 561
TauI GCSGC 2 cut(s) 63, 179
TfiI GAWTC 1 cut(s) 583
TscAI CASTG 3 cut(s) 104, 173, 673
TspDTI ATGAA 2 cut(s) 13, 611
TspGWI ACGGA 1 cut(s) 105
TspRI CASTG 3 cut(s) 104, 173, 673
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 230, 502
ZrmI AGTACT 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.