AT5G16520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001442
Physical Location & Seq
Reverse (-)
5394544 .. 5397466
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16520.2

Sequence Viewer

Length: 1827 bp
ATGGCGTGCCCTAGAAACTCCATAACATACAACGCCACGCGCTGCGCGTGCGGTATCGGTCAGCTACTAAACCGGAGCTCCGGGAGCTGCGAGATCTTCGGTTGGCCGTCAACAATCTCCACAGACAAAGATGTAAACTACAGCGTAATATCATTCGCCGAGACTTTATTCGCTTTCGATAGGATCCGTAAGTTCACGCAATCTCAAGCTATTTTCTTAGAAGCTACGCTCGTGATGCTTCTCTCCTGGTTAGTCTTTTGTTTCTTCCTGAGATTCACCAAACTCGGCGATGGTCGTAATGTCTGGTTTAATCTCCGTTGGTGGATTACTCGACTTGACGTCTTCTTCTCCACTAGACATTGGCTCGATGATCAACAAATTGTCAAGAAACGTAAAACAGAACTTGGTGGAACATTTTCTGTTGCGAGCTGGATTGTTTTCATTGGTTTATTCGCTGCGCTGCTTTATCAAATCATAACCAAGAGAACAATTGAAGTTCACAATGTGAGAGCTACTGGTTCTCCAGATTTGATCTCATTCGAGAACGATTTGGAGTTTAACATCACCGCTGTTTCGGATATGAGTTGCTCTAATTTACGCGGTATTGGGAACGTGGTCATGGGTAATCCCGGGTTTAGCGAGTTCAAAGTTGCTGCTCTTTCTAGTTTAGGAAGCTATACTTGTAAGAATACAACTTCAGGACCAACAGTGAATTTCAAATGTACCAAATGTCGTCTCACCAACGATTACATATATATCTCGTGGCATTTCGTTGATCTTCCTGATTCGCCTGCTGCTGCTGTTGGATTTCAGTTTAACTTTACGTCTAAGAATGGACCTAACGAGAAGCATATGAGTTTTGTTAGTGGCACTCTGAGAAACGGAAGCATTCTCGATGAAAGTCCGGTTACTTTCCGTGGAACCGAAGGGAATATATTGAAGTTTAATCTGTTTCCTCGGATTTACCATCATTTGCATGATCTAAAGCTAATCCAACCTCTCTTTCACGAGTTTATCCCTGGCTCTGTTTACCGAGACACTACTCAGCTTCAGGCATCTATGGGAAGGTCAACAGATGGCATACTCAATACGACATTGTTCATCAACTATCTCTCTGCTTATATTGTTGAGATAGACCATGAAAATATACTCGGTCCTGTTAGCTTCCTTGCCGATCTTGGTGGTTTGTATTGCATCAGTATTGGCATATTTTTCTACCTACTAGTGCAGTGTGAGTACAGGATCAAGAAGCTGCGAAATGAAGATACTGTTTTCAGGAAGATAAGGAATCGTCGTAAAGCTCTAGATCACTGGGATAAACTGAGAAGATATGTTGCATACACATGGGATTGCAGCATATTGGTCGATGATGCCATCAAAACGACAAAGGTATCAGGAATGTGTGGTTTAACGAGACCTCCAACATCTTCTAATAGCTCGGAACACGGGGAATCGATCATGGCGAACAAGAAGCCTAACTTAGGTATTGAGAAGAATGTGATTTCACAACCTGCAAGTTTAGAACTGAGCTCTTTTGATTCGGCTTCTTCTCTGGCTCATGGAGATAATTTCTCAAATAAAAAGAGTATAACTCATCCAATTTCTCATAGTGAAGACGTTTCAATCCCTCCACCTCCTCCAATGGAGTTCATTGATGGATCTTCTGGTTCAGAAGTGGACGCAATGGATATCAAGAATAAATTTCAACTTCTTTACGACTACAATGTATTGCTTAGGGAAAAACTTTTAGAGACTCAGTCTTTGCTTAATACTTTGGCACCCAAACCAATAGCATCATCATCTTCAACCGTGGAACGCAGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

608

Amino Acids

68.31

Weight (kDa)

8.3

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 342
Acc36I ACCTGC 1 cut(s) 1519
AccB1I GGYRCC 1 cut(s) 1777
AccII CGCG 3 cut(s) 40, 47, 600
AciI CCGC 3 cut(s) 51, 567, 600
AclWI GGATC 4 cut(s) 178, 191, 1250, 1666
AcoI YGGCCR 1 cut(s) 104
AcsI RAATTY 2 cut(s) 712, 1700
AcuI CTGAAG 2 cut(s) 681, 1034
AcyI GRCGYC 1 cut(s) 339
AdeI CACNNNGTG 1 cut(s) 505
AfaI GTAC 2 cut(s) 724, 1238
AfiI CCNNNNNNNGG 1 cut(s) 1481
AgsI TTSAA 7 cut(s) 494, 646, 718, 940, 1623, 1706, 1806
AhdI GACNNNNNGTC 1 cut(s) 338
AhlI ACTAGT 1 cut(s) 1222
AjnI CCWGG 2 cut(s) 245, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 987, 1019
Alw21I GWGCWC 2 cut(s) 80, 1532
Alw26I GTCTC 5 cut(s) 155, 740, 1029, 1408, 1745
AlwI GGATC 4 cut(s) 178, 191, 1250, 1666
Ama87I CYCGRG 1 cut(s) 629
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 9 cut(s) 42, 87, 455, 460, 653, 794, 797, 1252, 1353
ApoI RAATTY 2 cut(s) 712, 1700
ArsI GACNNNNNNTTYG 2 cut(s) 1744, 1776
Asp700I GAANNNNTTC 1 cut(s) 415
AspLEI GCGC 3 cut(s) 42, 47, 460
AspS9I GGNCC 3 cut(s) 701, 836, 1154
AsuC2I CCSGG 3 cut(s) 82, 630, 631
AsuHPI GGTGA 3 cut(s) 268, 556, 730
AvaI CYCGRG 1 cut(s) 629
AvaII GGWCC 3 cut(s) 701, 836, 1154
BaeGI GKGCMC 1 cut(s) 11
BaeI ACNNNNGTAYC 2 cut(s) 1374, 1407
BamHI GGATCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 1777
BanII GRGCYC 2 cut(s) 80, 1532
BauI CACGAG 3 cut(s) 230, 760, 1007
BbsI GAAGAC 2 cut(s) 334, 1620
Bbv12I GWGCWC 2 cut(s) 80, 1532
BbvI GCAGC 9 cut(s) 29, 74, 442, 447, 640, 781, 784, 1239, 1365
BccI CCATC 5 cut(s) 284, 975, 1070, 1382, 1649
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 2 cut(s) 1345, 1379
BciT130I CCWGG 2 cut(s) 247, 1020
BclI TGATCA 1 cut(s) 370
BcnI CCSGG 3 cut(s) 82, 630, 631
BcoDI GTCTC 5 cut(s) 155, 740, 1029, 1408, 1745
BcuI ACTAGT 1 cut(s) 1222
BfaI CTAG 6 cut(s) 12, 354, 663, 1223, 1304, 1825
BfmI CTRYAG 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 1519
BglII AGATCT 1 cut(s) 93
BisI GCNGC 9 cut(s) 43, 88, 456, 461, 654, 795, 798, 1253, 1354
BlsI GCNGC 9 cut(s) 44, 89, 457, 462, 655, 796, 799, 1254, 1355
Bme1390I CCNGG 5 cut(s) 82, 247, 630, 631, 1020
Bme18I GGWCC 3 cut(s) 701, 836, 1154
BmeRI GACNNNNNGTC 1 cut(s) 338
BmeT110I CYCGRG 1 cut(s) 629
BmgT120I GGNCC 3 cut(s) 701, 836, 1154
BmiI GGNNCC 3 cut(s) 185, 922, 1779
BmrFI CCNGG 5 cut(s) 82, 247, 630, 631, 1020
BmrI ACTGGG 1 cut(s) 1321
BmsI GCATC 5 cut(s) 225, 1064, 1203, 1360, 1802
BmuI ACTGGG 1 cut(s) 1321
BpiI GAAGAC 2 cut(s) 334, 1620
BplI GAGNNNNNCTC 2 cut(s) 1576, 1608
BpmI CTGGAG 1 cut(s) 507
Bpu10I CCTNAGC 1 cut(s) 1733
BpuEI CTTGAG 1 cut(s) 189
BpuMI CCSGG 3 cut(s) 82, 630, 631
Bsa29I ATCGAT 1 cut(s) 1454
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 1 cut(s) 1408
BsaJI CCNNGG 5 cut(s) 629, 916, 956, 1018, 1809
BsaWI WCCGGW 2 cut(s) 72, 904
BsaXI ACNNNNNCTCC 6 cut(s) 62, 92, 505, 535, 1402, 1432
Bsc4I CCNNNNNNNGG 1 cut(s) 1481
Bse1I ACTGG 2 cut(s) 520, 1316
Bse3DI GCAATG 1 cut(s) 1689
BseBI CCWGG 2 cut(s) 247, 1020
BseCI ATCGAT 1 cut(s) 1454
BseDI CCNNGG 5 cut(s) 629, 916, 956, 1018, 1809
BseGI GGATG 1 cut(s) 1594
BseLI CCNNNNNNNGG 1 cut(s) 1481
BseMI GCAATG 1 cut(s) 1689
BseMII CTCAG 6 cut(s) 260, 866, 1058, 1313, 1517, 1769
BseNI ACTGG 2 cut(s) 520, 1316
BseRI GAGGAG 1 cut(s) 1626
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 9 cut(s) 29, 74, 442, 447, 640, 781, 784, 1239, 1365
BsgI GTGCAG 1 cut(s) 1247
Bsh1236I CGCG 3 cut(s) 40, 47, 600
BshFI GGCC 1 cut(s) 106
BshNI GGYRCC 1 cut(s) 1777
BshVI ATCGAT 1 cut(s) 1454
BsiHKAI GWGCWC 2 cut(s) 80, 1532
BsiHKCI CYCGRG 1 cut(s) 629
BsiSI CCGG 4 cut(s) 73, 81, 630, 905
BslI CCNNNNNNNGG 1 cut(s) 1481
BsmAI GTCTC 5 cut(s) 155, 740, 1029, 1408, 1745
BsmBI CGTCTC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 1 cut(s) 106
Bso31I GGTCTC 1 cut(s) 1408
BsoBI CYCGRG 1 cut(s) 629
Bsp1286I GDGCHC 3 cut(s) 11, 80, 1532
BspACI CCGC 3 cut(s) 51, 567, 600
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 6 cut(s) 261, 867, 1057, 1314, 1518, 1768
BspDI ATCGAT 1 cut(s) 1454
BspFNI CGCG 3 cut(s) 40, 47, 600
BspLI GGNNCC 3 cut(s) 185, 922, 1779
BspMI ACCTGC 1 cut(s) 1519
BspPI GGATC 4 cut(s) 178, 191, 1250, 1666
BspT107I GGYRCC 1 cut(s) 1777
BspTNI GGTCTC 1 cut(s) 1408
BsrDI GCAATG 1 cut(s) 1689
BsrI ACTGG 2 cut(s) 520, 1316
BssECI CCNNGG 5 cut(s) 629, 916, 956, 1018, 1809
BssNI GRCGYC 1 cut(s) 339
BssSI CACGAG 3 cut(s) 230, 760, 1007
Bst2BI CACGAG 3 cut(s) 230, 760, 1007
Bst2UI CCWGG 2 cut(s) 247, 1020
Bst4CI ACNGT 3 cut(s) 709, 1270, 1810
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 4 cut(s) 7, 49, 427, 792
BstDSI CCRYGG 2 cut(s) 916, 1809
BstENI CCTNNNNNAGG 1 cut(s) 1479
BstF5I GGATG 1 cut(s) 1594
BstFNI CGCG 3 cut(s) 40, 47, 600
BstHHI GCGC 3 cut(s) 42, 47, 460
BstMAI GTCTC 5 cut(s) 155, 740, 1029, 1408, 1745
BstMWI GCNNNNNNNGC 3 cut(s) 48, 84, 235
BstNI CCWGG 2 cut(s) 247, 1020
BstSCI CCNGG 5 cut(s) 80, 245, 628, 629, 1018
BstSFI CTRYAG 1 cut(s) 139
BstSLI GKGCMC 1 cut(s) 11
BstUI CGCG 3 cut(s) 40, 47, 600
BstV1I GCAGC 9 cut(s) 29, 74, 442, 447, 640, 781, 784, 1239, 1365
BstV2I GAAGAC 2 cut(s) 334, 1620
BstX2I RGATCY 3 cut(s) 93, 183, 1658
BstYI RGATCY 3 cut(s) 93, 183, 1658
Bsu15I ATCGAT 1 cut(s) 1454
BsuRI GGCC 1 cut(s) 106
BsuTUI ATCGAT 1 cut(s) 1454
BtgI CCRYGG 2 cut(s) 916, 1809
BtgZI GCGATG 1 cut(s) 303
BtsCI GGATG 1 cut(s) 1594
BtsI GCAGTG 2 cut(s) 1235, 1825
BtsIMutI CAGTG 4 cut(s) 714, 1235, 1309, 1825
BveI ACCTGC 1 cut(s) 1519
Cac8I GCNNGC 4 cut(s) 7, 49, 427, 792
CfoI GCGC 3 cut(s) 42, 47, 460
Cfr13I GGNCC 3 cut(s) 701, 836, 1154
Cfr9I CCCGGG 1 cut(s) 629
ClaI ATCGAT 1 cut(s) 1454
CseI GACGC 1 cut(s) 1688
Csp6I GTAC 2 cut(s) 723, 1237
CviAII CATG 6 cut(s) 619, 977, 1139, 1344, 1459, 1559
CviQI GTAC 2 cut(s) 723, 1237
DraIII CACNNNGTG 1 cut(s) 505
DriI GACNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 104
Eam1105I GACNNNNNGTC 1 cut(s) 338
Ecl136II GAGCTC 2 cut(s) 78, 1530
Eco24I GRGCYC 2 cut(s) 80, 1532
Eco31I GGTCTC 1 cut(s) 1408
Eco32I GATATC 1 cut(s) 1690
Eco47I GGWCC 3 cut(s) 701, 836, 1154
Eco53kI GAGCTC 2 cut(s) 78, 1530
Eco57I CTGAAG 2 cut(s) 681, 1034
Eco88I CYCGRG 1 cut(s) 629
EcoICRI GAGCTC 2 cut(s) 78, 1530
EcoNI CCTNNNNNAGG 1 cut(s) 1479
EcoRII CCWGG 2 cut(s) 245, 1018
EcoRV GATATC 1 cut(s) 1690
EcoT38I GRGCYC 2 cut(s) 80, 1532
Esp3I CGTCTC 1 cut(s) 740
FaeI CATG 6 cut(s) 622, 980, 1142, 1347, 1462, 1562
FalI AAGNNNNNCTT 4 cut(s) 664, 696, 1463, 1495
FatI CATG 6 cut(s) 618, 976, 1138, 1343, 1458, 1558
FauNDI CATATG 1 cut(s) 852
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 9 cut(s) 43, 88, 456, 461, 654, 795, 798, 1253, 1354
FokI GGATG 1 cut(s) 1581
FriOI GRGCYC 2 cut(s) 80, 1532
Fsp4HI GCNGC 9 cut(s) 43, 88, 456, 461, 654, 795, 798, 1253, 1354
FspBI CTAG 6 cut(s) 12, 354, 663, 1223, 1304, 1825
GlaI GCGC 3 cut(s) 41, 46, 459
GluI GCNGC 9 cut(s) 43, 88, 456, 461, 654, 795, 798, 1253, 1354
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 106
HapII CCGG 4 cut(s) 73, 81, 630, 905
HgaI GACGC 1 cut(s) 1688
HhaI GCGC 3 cut(s) 42, 47, 460
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 6 cut(s) 622, 980, 1142, 1347, 1462, 1562
Hin6I GCGC 3 cut(s) 40, 45, 458
HinP1I GCGC 3 cut(s) 40, 45, 458
HincII GTYRAC 2 cut(s) 111, 1071
HindII GTYRAC 2 cut(s) 111, 1071
HinfI GANTC 6 cut(s) 273, 785, 1288, 1451, 1538, 1753
HpaII CCGG 4 cut(s) 73, 81, 630, 905
HphI GGTGA 3 cut(s) 268, 556, 730
Hpy166II GTNNAC 7 cut(s) 111, 136, 195, 499, 1030, 1071, 1678
Hpy188I TCNGA 5 cut(s) 577, 876, 960, 1441, 1672
Hpy8I GTNNAC 7 cut(s) 111, 136, 195, 499, 1030, 1071, 1678
Hpy99I CGWCG 1 cut(s) 1296
HpyAV CCTTC 2 cut(s) 920, 1059
HpyCH4III ACNGT 3 cut(s) 709, 1270, 1810
HpyCH4IV ACGT 5 cut(s) 339, 391, 612, 824, 1617
HpyCH4V TGCA 6 cut(s) 976, 1194, 1228, 1337, 1353, 1514
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 84, 235
HpySE526I ACGT 5 cut(s) 339, 391, 612, 824, 1617
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 6 cut(s) 622, 980, 1142, 1347, 1462, 1562
HspAI GCGC 3 cut(s) 40, 45, 458
Ksp22I TGATCA 1 cut(s) 370
LmnI GCTCC 3 cut(s) 75, 83, 84
Lsp1109I GCAGC 9 cut(s) 29, 74, 442, 447, 640, 781, 784, 1239, 1365
LweI GCATC 5 cut(s) 225, 1064, 1203, 1360, 1802
MaeI CTAG 6 cut(s) 12, 354, 663, 1223, 1304, 1825
MaeII ACGT 5 cut(s) 339, 391, 612, 824, 1617
MaeIII GTNAC 1 cut(s) 907
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 3 cut(s) 93, 183, 1658
MhlI GDGCHC 3 cut(s) 11, 80, 1532
MluCI AATT 7 cut(s) 378, 489, 592, 712, 1567, 1599, 1700
MlyI GAGTC 1 cut(s) 1747
MmeI TCCRAC 3 cut(s) 784, 1018, 1445
MnlI CCTC 6 cut(s) 966, 1008, 1428, 1638, 1644, 1647
MroXI GAANNNNTTC 1 cut(s) 415
MseI TTAA 6 cut(s) 309, 558, 816, 945, 1409, 1767
MslI CAYNNNNRTG 2 cut(s) 975, 1342
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 4 cut(s) 73, 81, 630, 905
MspR9I CCNGG 5 cut(s) 82, 247, 630, 631, 1020
MteI GCGCNGCGC 1 cut(s) 43
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 2 cut(s) 247, 1020
MvnI CGCG 3 cut(s) 40, 47, 600
MwoI GCNNNNNNNGC 3 cut(s) 48, 84, 235
NciI CCSGG 3 cut(s) 82, 630, 631
NdeI CATATG 1 cut(s) 852
NlaIII CATG 6 cut(s) 622, 980, 1142, 1347, 1462, 1562
NlaIV GGNNCC 3 cut(s) 185, 922, 1779
NmeAIII GCCGAG 2 cut(s) 184, 264
PcsI WCGNNNNNNNCGW 2 cut(s) 44, 1460
PctI GAATGC 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 415
PfeI GAWTC 5 cut(s) 273, 785, 1288, 1451, 1538
PflFI GACNNNGTC 1 cut(s) 1756
PfoI TCCNGGA 1 cut(s) 80
PkrI GCNGC 9 cut(s) 44, 89, 457, 462, 655, 796, 799, 1254, 1355
PleI GAGTC 1 cut(s) 1747
PpsI GAGTC 1 cut(s) 1747
Psp124BI GAGCTC 2 cut(s) 80, 1532
Psp6I CCWGG 2 cut(s) 245, 1018
PspGI CCWGG 2 cut(s) 245, 1018
PspN4I GGNNCC 3 cut(s) 185, 922, 1779
PspPI GGNCC 3 cut(s) 701, 836, 1154
PsuI RGATCY 3 cut(s) 93, 183, 1658
PsyI GACNNNGTC 1 cut(s) 1756
RsaI GTAC 2 cut(s) 724, 1238
RsaNI GTAC 2 cut(s) 723, 1237
RseI CAYNNNNRTG 2 cut(s) 975, 1342
SacI GAGCTC 2 cut(s) 80, 1532
SaqAI TTAA 6 cut(s) 309, 558, 816, 945, 1409, 1767
SatI GCNGC 9 cut(s) 43, 88, 456, 461, 654, 795, 798, 1253, 1354
Sau96I GGNCC 3 cut(s) 701, 836, 1154
SchI GAGTC 1 cut(s) 1747
ScrFI CCNGG 5 cut(s) 82, 247, 630, 631, 1020
SduI GDGCHC 3 cut(s) 11, 80, 1532
SfaNI GCATC 5 cut(s) 225, 1064, 1203, 1360, 1802
SfcI CTRYAG 1 cut(s) 139
SinI GGWCC 3 cut(s) 701, 836, 1154
SmaI CCCGGG 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 975, 1342
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
SpeI ACTAGT 1 cut(s) 1222
Sse9I AATT 7 cut(s) 378, 489, 592, 712, 1567, 1599, 1700
SsiI CCGC 3 cut(s) 51, 567, 600
SspMI CTAG 6 cut(s) 12, 354, 663, 1223, 1304, 1825
SstI GAGCTC 2 cut(s) 80, 1532
StyD4I CCNGG 5 cut(s) 80, 245, 628, 629, 1018
TaaI ACNGT 3 cut(s) 709, 1270, 1810
TaiI ACGT 5 cut(s) 342, 394, 615, 827, 1620
TaqI TCGA 7 cut(s) 177, 331, 366, 540, 894, 1365, 1454
TaqII GACCGA 2 cut(s) 47, 1142
TasI AATT 7 cut(s) 378, 489, 592, 712, 1567, 1599, 1700
TatI WGTACW 1 cut(s) 1236
TfiI GAWTC 5 cut(s) 273, 785, 1288, 1451, 1538
Tru1I TTAA 6 cut(s) 309, 558, 816, 945, 1409, 1767
Tru9I TTAA 6 cut(s) 309, 558, 816, 945, 1409, 1767
TscAI CASTG 4 cut(s) 714, 1235, 1316, 1825
TseI GCWGC 9 cut(s) 42, 87, 455, 460, 653, 794, 797, 1252, 1353
TspDTI ATGAA 6 cut(s) 430, 912, 1090, 1155, 1275, 1639
TspGWI ACGGA 4 cut(s) 176, 305, 897, 905
TspMI CCCGGG 1 cut(s) 629
TspRI CASTG 4 cut(s) 714, 1235, 1316, 1825
Tth111I GACNNNGTC 1 cut(s) 1756
VpaK11BI GGWCC 3 cut(s) 701, 836, 1154
XagI CCTNNNNNAGG 1 cut(s) 1479
XapI RAATTY 2 cut(s) 712, 1700
XbaI TCTAGA 1 cut(s) 1303
XmaI CCCGGG 1 cut(s) 629
XmnI GAANNNNTTC 1 cut(s) 415
XspI CTAG 6 cut(s) 12, 354, 663, 1223, 1304, 1825
ZraI GACGTC 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.