RLG00000032394

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001442
Physical Location & Seq
Reverse (-)
14373385 .. 14376521
3137 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032394

Sequence Viewer

Length: 594 bp
ATGTCAAGGAAGCACACTGAGCTGCCGCTCCATCCTATTGGAGAAGAAAGGCATGTGTGTGATTGTGCCGTGATATATCTTGGTCTGCACTCCACCTCCATCCTATTGGAGAAGAAGGGCATGTGTGTGATTGTGCTTAAGTTCATTGATCTTCCTGATAATCCTGCTGCTGCTGTTGGATTTCAGTTCAATCTTACTGCAAGGAGTCATGCTAATAAAAGACATCTGAGTTTTGTTAGTGGAACCCTAAAGAATGGAAGTACTTTGGATGATAGACCAGTTACATTCAGAGGGAGTAATACAAATATATTGAAATTCAGTTTATTTCCACGGATATACCATAATCTACATGATCTAAGGCTCATCCAACCTTTGTTTCATGTGTTTGTACTGGGTTCATTCTTTAATGACACAAGTCAGCTCCAAGCATCCCTTCAAAGGCCTAAGGATGGAATACTTAACACCACATTGTACATCAACTTCCTCTCTGACTACATTGTGGAGATAGAGAAGCAAAATATTATGGGTCCTGGAAGACAACAGGTGCTTCCCTTAAGTAAACAAGATCCATCCTTTCAGTATGTTCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.3

Weight (kDa)

8.93

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 28
AciI CCGC 1 cut(s) 26
AclWI GGATC 1 cut(s) 560
AcsI RAATTY 1 cut(s) 314
AfaI GTAC 3 cut(s) 262, 390, 473
AfiI CCNNNNNNNGG 2 cut(s) 438, 449
AflII CTTAAG 2 cut(s) 137, 553
AgsI TTSAA 3 cut(s) 190, 313, 437
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 4 cut(s) 360, 392, 417, 449
AluBI AGCT 2 cut(s) 22, 421
AluI AGCT 2 cut(s) 22, 421
AlwI GGATC 1 cut(s) 560
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 3 cut(s) 22, 167, 170
ApoI RAATTY 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 527
AvaII GGWCC 1 cut(s) 527
AxyI CCTNAGG 1 cut(s) 444
BarI GAAGNNNNNNTAC 2 cut(s) 464, 496
BbsI GAAGAC 1 cut(s) 541
BbvI GCAGC 3 cut(s) 9, 154, 157
BccI CCATC 4 cut(s) 39, 107, 443, 577
BceAI ACGGC 1 cut(s) 53
BciT130I CCWGG 1 cut(s) 531
BfrI CTTAAG 2 cut(s) 137, 553
BisI GCNGC 4 cut(s) 23, 26, 168, 171
BlsI GCNGC 4 cut(s) 24, 27, 169, 172
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 531
Bme18I GGWCC 1 cut(s) 527
BmgT120I GGNCC 1 cut(s) 527
BmiI GGNNCC 2 cut(s) 244, 528
BmrFI CCNGG 1 cut(s) 531
BmrI ACTGGG 1 cut(s) 401
BmsI GCATC 1 cut(s) 437
BmuI ACTGGG 1 cut(s) 401
BoxI GACNNNNGTC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 541
BsaJI CCNNGG 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 449
Bse1I ACTGG 2 cut(s) 278, 396
Bse21I CCTNAGG 1 cut(s) 444
BseBI CCWGG 1 cut(s) 531
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 7 cut(s) 31, 99, 274, 363, 428, 454, 569
BseLI CCNNNNNNNGG 2 cut(s) 438, 449
BseMII CTCAG 2 cut(s) 9, 218
BseNI ACTGG 2 cut(s) 278, 396
BseXI GCAGC 3 cut(s) 9, 154, 157
BsgI GTGCAG 1 cut(s) 71
BshFI GGCC 1 cut(s) 442
BslI CCNNNNNNNGG 2 cut(s) 438, 449
BsnI GGCC 1 cut(s) 442
Bsp1407I TGTACA 1 cut(s) 471
Bsp143I GATC 3 cut(s) 148, 352, 565
BspACI CCGC 1 cut(s) 26
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 2 cut(s) 10, 219
BspLI GGNNCC 2 cut(s) 244, 528
BspPI GGATC 1 cut(s) 560
BspTI CTTAAG 2 cut(s) 137, 553
BsrBI CCGCTC 1 cut(s) 28
BsrGI TGTACA 1 cut(s) 471
BsrI ACTGG 2 cut(s) 278, 396
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 3 cut(s) 148, 352, 565
Bst2UI CCWGG 1 cut(s) 531
BstAFI CTTAAG 2 cut(s) 137, 553
BstAUI TGTACA 1 cut(s) 471
BstDEI CTNAG 4 cut(s) 18, 227, 356, 444
BstDSI CCRYGG 1 cut(s) 329
BstF5I GGATG 7 cut(s) 31, 99, 274, 363, 428, 454, 569
BstKTI GATC 3 cut(s) 151, 355, 568
BstMBI GATC 3 cut(s) 148, 352, 565
BstMWI GCNNNNNNNGC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 531
BstNSI RCATGY 2 cut(s) 56, 124
BstPAI GACNNNNGTC 1 cut(s) 414
BstSCI CCNGG 1 cut(s) 529
BstV1I GCAGC 3 cut(s) 9, 154, 157
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 565
BstXI CCANNNNNNTGG 2 cut(s) 38, 106
BstYI RGATCY 1 cut(s) 565
Bsu36I CCTNAGG 1 cut(s) 444
BsuRI GGCC 1 cut(s) 442
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 7 cut(s) 31, 99, 274, 363, 428, 454, 569
BtsIMutI CAGTG 1 cut(s) 15
Cfr13I GGNCC 1 cut(s) 527
Csp6I GTAC 3 cut(s) 261, 389, 472
CviAII CATG 6 cut(s) 53, 121, 209, 350, 380, 588
CviJI RGCY 4 cut(s) 22, 361, 421, 442
CviKI_1 RGCY 4 cut(s) 22, 361, 421, 442
CviQI GTAC 3 cut(s) 261, 389, 472
DdeI CTNAG 4 cut(s) 18, 227, 356, 444
DpnI GATC 3 cut(s) 150, 354, 567
DpnII GATC 3 cut(s) 148, 352, 565
Eco147I AGGCCT 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 527
Eco81I CCTNAGG 1 cut(s) 444
EcoO109I RGGNCCY 1 cut(s) 527
EcoRII CCWGG 1 cut(s) 529
FaeI CATG 6 cut(s) 56, 124, 212, 353, 383, 591
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 6 cut(s) 52, 120, 208, 349, 379, 587
Fnu4HI GCNGC 4 cut(s) 23, 26, 168, 171
FokI GGATG 7 cut(s) 18, 86, 281, 350, 415, 461, 556
Fsp4HI GCNGC 4 cut(s) 23, 26, 168, 171
GluI GCNGC 4 cut(s) 23, 26, 168, 171
HaeIII GGCC 1 cut(s) 442
Hin1II CATG 6 cut(s) 56, 124, 212, 353, 383, 591
HinfI GANTC 1 cut(s) 205
Hpy166II GTNNAC 1 cut(s) 560
Hpy188I TCNGA 3 cut(s) 228, 290, 490
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 560
HpyAV CCTTC 2 cut(s) 109, 443
HpyCH4V TGCA 2 cut(s) 88, 200
HpyF10VI GCNNNNNNNGC 1 cut(s) 19
HpyF3I CTNAG 4 cut(s) 18, 227, 356, 444
Hsp92II CATG 6 cut(s) 56, 124, 212, 353, 383, 591
Kzo9I GATC 3 cut(s) 148, 352, 565
LmnI GCTCC 2 cut(s) 33, 426
LpnPI CCDG 7 cut(s) 168, 177, 291, 377, 516, 527, 543
Lsp1109I GCAGC 3 cut(s) 9, 154, 157
LweI GCATC 1 cut(s) 437
MaeIII GTNAC 1 cut(s) 280
MalI GATC 3 cut(s) 150, 354, 567
MbiI CCGCTC 1 cut(s) 28
MboI GATC 3 cut(s) 148, 352, 565
MboII GAAGA 4 cut(s) 56, 124, 143, 546
MflI RGATCY 1 cut(s) 565
MluCI AATT 1 cut(s) 314
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 2 cut(s) 157, 391
MnlI CCTC 3 cut(s) 106, 284, 494
MseI TTAA 5 cut(s) 138, 405, 459, 554, 592
MslI CAYNNNNRTG 2 cut(s) 57, 125
MspCI CTTAAG 2 cut(s) 137, 553
MspR9I CCNGG 1 cut(s) 531
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 19
NdeII GATC 3 cut(s) 148, 352, 565
NlaIII CATG 6 cut(s) 56, 124, 212, 353, 383, 591
NlaIV GGNNCC 2 cut(s) 244, 528
NspI RCATGY 2 cut(s) 56, 124
PceI AGGCCT 1 cut(s) 442
PfoI TCCNGGA 1 cut(s) 529
PkrI GCNGC 4 cut(s) 24, 27, 169, 172
PleI GAGTC 1 cut(s) 213
PpsI GAGTC 1 cut(s) 213
PpuMI RGGWCCY 1 cut(s) 527
PshAI GACNNNNGTC 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 527
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
PspN4I GGNNCC 2 cut(s) 244, 528
PspPI GGNCC 1 cut(s) 527
PspPPI RGGWCCY 1 cut(s) 527
PsuI RGATCY 1 cut(s) 565
RsaI GTAC 3 cut(s) 262, 390, 473
RsaNI GTAC 3 cut(s) 261, 389, 472
RseI CAYNNNNRTG 2 cut(s) 57, 125
SaqAI TTAA 5 cut(s) 138, 405, 459, 554, 592
SatI GCNGC 4 cut(s) 23, 26, 168, 171
Sau3AI GATC 3 cut(s) 148, 352, 565
Sau96I GGNCC 1 cut(s) 527
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 531
SetI ASST 5 cut(s) 24, 98, 373, 423, 546
SfaNI GCATC 1 cut(s) 437
SinI GGWCC 1 cut(s) 527
SmiMI CAYNNNNRTG 2 cut(s) 57, 125
SmlI CTYRAG 2 cut(s) 137, 553
SmoI CTYRAG 2 cut(s) 137, 553
Sse9I AATT 1 cut(s) 314
SseBI AGGCCT 1 cut(s) 442
SsiI CCGC 1 cut(s) 26
SspI AATATT 1 cut(s) 520
StuI AGGCCT 1 cut(s) 442
StyD4I CCNGG 1 cut(s) 529
TasI AATT 1 cut(s) 314
TatI WGTACW 3 cut(s) 260, 388, 471
TauI GCSGC 1 cut(s) 28
Tru1I TTAA 5 cut(s) 138, 405, 459, 554, 592
Tru9I TTAA 5 cut(s) 138, 405, 459, 554, 592
TscAI CASTG 1 cut(s) 22
TseI GCWGC 3 cut(s) 22, 167, 170
TspDTI ATGAA 3 cut(s) 133, 368, 387
TspGWI ACGGA 1 cut(s) 346
TspRI CASTG 1 cut(s) 22
Vha464I CTTAAG 2 cut(s) 137, 553
VpaK11BI GGWCC 1 cut(s) 527
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 56, 124
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.