Prupe.6G359100_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001442
Physical Location & Seq
Forward (+)
30364089 .. 30369712
5624 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G359100.1

Sequence Viewer

Length: 1917 bp
ATGTCATGCCCATCATCAAACAGCATCAGGTACAATGGGAGCCACTGCACATGCGCAGTTGGGTATTTGCTAAATCAGACCACCGACACCTGCGTCCTCTTTACAGCGGATTCCACCATATCCACCGACTCCGGCATCAGCACCAACGTCCTCAGCTTCTTCCCGGAGACCATCTTCTCCTTTGACTCCATTAAGAAGTTCACGCAATCCCAAGCCGTCTTCTTGGAAGCCACGCTTGTGTTTCTGCTGTCTTGGCTTTTCTTTTGCTTCTTTCTAAGGTTTATGAAGCTTGGCAACGATGGCAGGAGCATTTGGTTCAGGCTTAGATGGTGGATTAGCAGATTGGATGTTTGCTTTTCCACTAGGCATTGGCTGGATGATCAAAAGGTAGTTAAGAAACGGAAGACAGAACTTGGTGGAACCTTCTCAATAGCAAGTTGGATTCTCTTCATTGGTTTGTTTGCTGCGTTGCTCTACCAAATCATAACAAAGAGAACTATCGAGGTACATAATGTCCGAGCAACAAATGCACCCGACTTGGCCTCCTTCAACAATGATATGGAATTTAACATAACCACTATTTCTAGTATGAGCTGTTCAAACTTACGTGATCTTGCCACTTTAGTTACTGGAAATCCTGGCTTCATTGACTTTAGAGTGGCTCCTCTGTCAACATTTGGGAACTACTCATGTCAAAATACGAGTAGAGGACCAATGATAACTCTGAGGTGCAACAATTGTCAACCAATTCAAGACAATTTGTACATCTCGTGGCAATTCATTGATCTTCCTAATAATCCTGCTGCTGCTGTTGGATTTCAGTTCAATCTGAGTACAAGGAGTCATGCTAATAAAAAACATGTTAGTTTTGTTAGTGGAACCCTAAAGAATGGAAGCACTTTTGATGATAGACCAGTTACATTCAGAGGGAATGTGACAAACATTTTAAAATTCAGTTTATTCCCACGGATATACCGTAATCTGCATGGTCTAAAGCACATCCAACCTCTGTTTCATGACTTTGTTCCTGGTTCATTTTTCCGTGACACAAGTCAGCTCCAAACATCCCTTGAAAGTTCTAACGACGGAATACTCAACGCTACATTGTATGTCAACTTTCTCTCTGCCTACATTGTGGAGATAGATAACCGGAACATTATGGGTCCTGTGAGCTTCCTTGCGGATCTTGGTGGCCTGTATTGCATCTGTATTGGCATTTTTTTCTACCTTCTGGTGCAATGTGAGTTTAGGATAAAAAAACTCCGCAATGAAGATAGTGTATTGCGCAAGATCAGAAATCGGCGAAAAGCTCAAGATCACTGGGATAAATTGAGGAAATATGTGATGTACACATGGGGCTGCAAGGCATTGAATGAGAGCGAAAAAAAGGGATCTGGTTGCATTGGTTTCAATATACAATCAGTTCATCGAAATGGATCATCACACAAGCAAAGGCAACAAGAAATGGATACTATTAGTTTCAATAGGAAGCTCAGCTTACCTAACAAGAAGACTGCAGTTCAAGAGTGCTCCCACACTGTTGGGGGGGTGAGATCATTTACGCATGGAACTTCACTGAACCCTGTAGAAAGTTCATCACATTCTGCAGTTGAACCTCCTCTGCAAATTGAATTGCTTGGCTCTACAAAGGATGGAAAACACAAATGTGTTTGTTTGTGCAAAGAAGATTCCTCCCTGCCTAAAGCTTTAACTCTGACAAATGATGATATTATTCCTCCACCAGTTTCACTAGAATTGAAGGATGCTTCTGAAATGGATATGTCTGATGTTCAGAAGAATCTTAGAAGCTTGTACGAGTACAATGTTATGCTTAGGGAAAAGTTAATAGCTACTCAGTCTTTGTTCCATTCTTTACCTACAAATTCCGCATCCTCAGTAACAGAAGGCGAAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

639

Amino Acids

72.03

Weight (kDa)

8.9

Isoelectric Point (pI)

43.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 98
AasI GACNNNNNNGTC 1 cut(s) 92
Acc16I TGCGCA 2 cut(s) 55, 1286
Acc36I ACCTGC 1 cut(s) 98
AciI CCGC 4 cut(s) 107, 1181, 1264, 1887
AclWI GGATC 3 cut(s) 1191, 1399, 1444
AcsI RAATTY 3 cut(s) 563, 950, 1882
AfaI GTAC 7 cut(s) 32, 507, 764, 835, 1349, 1814, 1820
AflIII ACRYGT 1 cut(s) 859
AjnI CCWGG 2 cut(s) 637, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 996, 1028
AleI CACNNNNGTG 2 cut(s) 236, 1665
Alw21I GWGCWC 1 cut(s) 1532
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 3 cut(s) 1191, 1399, 1444
AoxI GGCC 2 cut(s) 540, 1192
ApeKI GCWGC 4 cut(s) 464, 803, 806, 1359
ApoI RAATTY 3 cut(s) 563, 950, 1882
AspLEI GCGC 2 cut(s) 56, 1287
AspS9I GGNCC 2 cut(s) 710, 1163
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 1561
AvaII GGWCC 2 cut(s) 710, 1163
BauI CACGAG 1 cut(s) 769
BbsI GAAGAC 3 cut(s) 211, 410, 1517
Bbv12I GWGCWC 1 cut(s) 1532
BbvCI CCTCAGC 1 cut(s) 152
BbvI GCAGC 4 cut(s) 451, 790, 793, 1346
BccI CCATC 5 cut(s) 19, 179, 293, 321, 1646
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 2 cut(s) 639, 1029
BciVI GTATCC 1 cut(s) 1462
BclI TGATCA 1 cut(s) 379
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 3 cut(s) 363, 585, 1751
BfmI CTRYAG 3 cut(s) 1515, 1584, 1605
BfuAI ACCTGC 1 cut(s) 98
BfuI GTATCC 1 cut(s) 1462
BisI GCNGC 4 cut(s) 465, 804, 807, 1360
BlpI GCTNAGC 1 cut(s) 1493
BlsI GCNGC 4 cut(s) 466, 805, 808, 1361
Bme1390I CCNGG 3 cut(s) 164, 639, 1029
Bme18I GGWCC 2 cut(s) 710, 1163
BmgT120I GGNCC 2 cut(s) 710, 1163
BmiI GGNNCC 5 cut(s) 41, 421, 663, 880, 1164
BmrFI CCNGG 3 cut(s) 164, 639, 1029
BmrI ACTGGG 1 cut(s) 1330
BmsI GCATC 5 cut(s) 33, 144, 1212, 1753, 1898
BmuI ACTGGG 1 cut(s) 1330
BoxI GACNNNNGTC 1 cut(s) 1050
BpiI GAAGAC 3 cut(s) 211, 410, 1517
Bpu10I CCTNAGC 2 cut(s) 152, 1832
Bpu1102I GCTNAGC 1 cut(s) 1493
BpuEI CTTGAG 1 cut(s) 1296
BpuMI CCSGG 1 cut(s) 164
BsaAI YACGTR 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 965
BsaWI WCCGGW 1 cut(s) 1149
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bse1I ACTGG 4 cut(s) 634, 914, 1325, 1742
Bse3DI GCAATG 2 cut(s) 1244, 1273
BseBI CCWGG 2 cut(s) 639, 1029
BseDI CCNNGG 1 cut(s) 965
BseGI GGATG 7 cut(s) 352, 382, 999, 1064, 1657, 1768, 1889
BseMI GCAATG 2 cut(s) 1244, 1273
BseMII CTCAG 6 cut(s) 166, 716, 821, 1507, 1868, 1908
BseNI ACTGG 4 cut(s) 634, 914, 1325, 1742
BseRI GAGGAG 2 cut(s) 654, 1608
BseXI GCAGC 4 cut(s) 451, 790, 793, 1346
BsgI GTGCAG 1 cut(s) 31
BshFI GGCC 2 cut(s) 542, 1194
BsiHKAI GWGCWC 1 cut(s) 1532
BsiSI CCGG 3 cut(s) 132, 164, 1150
BsmAI GTCTC 1 cut(s) 161
BsnI GGCC 2 cut(s) 542, 1194
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 1532
Bsp1407I TGTACA 2 cut(s) 762, 1347
Bsp143I GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
Bsp1720I GCTNAGC 1 cut(s) 1493
BspACI CCGC 4 cut(s) 107, 1181, 1264, 1887
BspANI GGCC 2 cut(s) 542, 1194
BspCNI CTCAG 6 cut(s) 165, 717, 822, 1506, 1867, 1907
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 5 cut(s) 41, 421, 663, 880, 1164
BspMAI CTGCAG 2 cut(s) 1519, 1609
BspMI ACCTGC 1 cut(s) 98
BspPI GGATC 3 cut(s) 1191, 1399, 1444
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 2 cut(s) 1244, 1273
BsrGI TGTACA 2 cut(s) 762, 1347
BsrI ACTGG 4 cut(s) 634, 914, 1325, 1742
BssECI CCNNGG 1 cut(s) 965
BssMI GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
BssSI CACGAG 1 cut(s) 769
Bst2BI CACGAG 1 cut(s) 769
Bst2UI CCWGG 2 cut(s) 639, 1029
Bst4CI ACNGT 2 cut(s) 977, 1540
Bst6I CTCTTC 1 cut(s) 452
BstAPI GCANNNNNTGC 1 cut(s) 527
BstAUI TGTACA 2 cut(s) 762, 1347
BstBAI YACGTR 1 cut(s) 608
BstDSI CCRYGG 1 cut(s) 965
BstF5I GGATG 7 cut(s) 352, 382, 999, 1064, 1657, 1768, 1889
BstHHI GCGC 2 cut(s) 56, 1287
BstKTI GATC 9 cut(s) 382, 613, 787, 1186, 1293, 1318, 1394, 1439, 1556
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
BstMWI GCNNNNNNNGC 4 cut(s) 253, 300, 527, 1200
BstNI CCWGG 2 cut(s) 639, 1029
BstNSI RCATGY 2 cut(s) 54, 863
BstPAI GACNNNNGTC 1 cut(s) 1050
BstSCI CCNGG 3 cut(s) 162, 637, 1027
BstSFI CTRYAG 3 cut(s) 1515, 1584, 1605
BstV1I GCAGC 4 cut(s) 451, 790, 793, 1346
BstV2I GAAGAC 3 cut(s) 211, 410, 1517
BstX2I RGATCY 2 cut(s) 1183, 1391
BstXI CCANNNNNNTGG 1 cut(s) 1541
BstYI RGATCY 2 cut(s) 1183, 1391
BsuI GTATCC 1 cut(s) 1462
BsuRI GGCC 2 cut(s) 542, 1194
BtgI CCRYGG 1 cut(s) 965
BtsCI GGATG 7 cut(s) 352, 382, 999, 1064, 1657, 1768, 1889
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 4 cut(s) 43, 1318, 1536, 1574
BveI ACCTGC 1 cut(s) 98
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 2 cut(s) 56, 1287
Cfr13I GGNCC 2 cut(s) 710, 1163
CseI GACGC 1 cut(s) 82
Csp6I GTAC 7 cut(s) 31, 506, 763, 834, 1348, 1813, 1819
CviAII CATG 9 cut(s) 6, 51, 690, 845, 860, 986, 1016, 1353, 1565
CviQI GTAC 7 cut(s) 31, 506, 763, 834, 1348, 1813, 1819
DpnI GATC 9 cut(s) 381, 612, 786, 1185, 1292, 1317, 1393, 1438, 1555
DpnII GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
DraI TTTAAA 1 cut(s) 948
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 2 cut(s) 710, 1163
EcoO109I RGGNCCY 1 cut(s) 1163
EcoRII CCWGG 2 cut(s) 637, 1027
FaeI CATG 9 cut(s) 9, 54, 693, 848, 863, 989, 1019, 1356, 1568
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 1481, 1513
FatI CATG 9 cut(s) 5, 50, 689, 844, 859, 985, 1015, 1352, 1564
FbaI TGATCA 1 cut(s) 379
Fnu4HI GCNGC 4 cut(s) 465, 804, 807, 1360
FokI GGATG 7 cut(s) 359, 389, 986, 1051, 1664, 1775, 1876
Fsp4HI GCNGC 4 cut(s) 465, 804, 807, 1360
FspBI CTAG 3 cut(s) 363, 585, 1751
FspI TGCGCA 2 cut(s) 55, 1286
GlaI GCGC 2 cut(s) 55, 1286
GluI GCNGC 4 cut(s) 465, 804, 807, 1360
HaeIII GGCC 2 cut(s) 542, 1194
HapII CCGG 3 cut(s) 132, 164, 1150
HgaI GACGC 1 cut(s) 82
HhaI GCGC 2 cut(s) 56, 1287
Hin1II CATG 9 cut(s) 9, 54, 693, 848, 863, 989, 1019, 1356, 1568
Hin6I GCGC 2 cut(s) 54, 1285
HinP1I GCGC 2 cut(s) 54, 1285
HincII GTYRAC 3 cut(s) 672, 743, 1114
HindII GTYRAC 3 cut(s) 672, 743, 1114
HindIII AAGCTT 3 cut(s) 287, 1704, 1807
HinfI GANTC 7 cut(s) 110, 128, 185, 442, 841, 1688, 1798
HpaII CCGG 3 cut(s) 132, 164, 1150
HphI GGTGA 1 cut(s) 1561
Hpy166II GTNNAC 5 cut(s) 201, 672, 743, 1114, 1350
Hpy188III TCNNGA 4 cut(s) 752, 1016, 1313, 1523
Hpy8I GTNNAC 5 cut(s) 201, 672, 743, 1114, 1350
Hpy99I CGWCG 1 cut(s) 1088
HpyAV CCTTC 5 cut(s) 433, 556, 1238, 1753, 1898
HpyCH4III ACNGT 2 cut(s) 977, 1540
HpyCH4IV ACGT 3 cut(s) 147, 607, 1913
HpyF10VI GCNNNNNNNGC 4 cut(s) 253, 300, 527, 1200
HpySE526I ACGT 3 cut(s) 147, 607, 1913
Hsp92II CATG 9 cut(s) 9, 54, 693, 848, 863, 989, 1019, 1356, 1568
HspAI GCGC 2 cut(s) 54, 1285
Ksp22I TGATCA 1 cut(s) 379
Kzo9I GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
LmnI GCTCC 5 cut(s) 39, 306, 667, 1062, 1535
Lsp1109I GCAGC 4 cut(s) 451, 790, 793, 1346
LweI GCATC 5 cut(s) 33, 144, 1212, 1753, 1898
MaeI CTAG 3 cut(s) 363, 585, 1751
MaeII ACGT 3 cut(s) 147, 607, 1913
MaeIII GTNAC 5 cut(s) 625, 916, 934, 1043, 1897
MalI GATC 9 cut(s) 381, 612, 786, 1185, 1292, 1317, 1393, 1438, 1555
MboI GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 2 cut(s) 1183, 1391
MhlI GDGCHC 1 cut(s) 1532
MlyI GAGTC 3 cut(s) 122, 179, 850
MmeI TCCRAC 3 cut(s) 419, 793, 1027
MseI TTAA 6 cut(s) 192, 393, 567, 947, 1709, 1844
MslI CAYNNNNRTG 3 cut(s) 236, 1431, 1665
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 3 cut(s) 132, 164, 1150
MspR9I CCNGG 3 cut(s) 164, 639, 1029
MunI CAATTG 1 cut(s) 736
MvaI CCWGG 2 cut(s) 639, 1029
MwoI GCNNNNNNNGC 4 cut(s) 253, 300, 527, 1200
NciI CCSGG 1 cut(s) 164
NdeII GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
NlaIII CATG 9 cut(s) 9, 54, 693, 848, 863, 989, 1019, 1356, 1568
NlaIV GGNNCC 5 cut(s) 41, 421, 663, 880, 1164
NmuCI GTSAC 2 cut(s) 934, 1043
NsbI TGCGCA 2 cut(s) 55, 1286
NspI RCATGY 2 cut(s) 54, 863
OliI CACNNNNGTG 2 cut(s) 236, 1665
PagI TCATGA 1 cut(s) 1015
PaqCI CACCTGC 1 cut(s) 98
PciI ACATGT 1 cut(s) 859
PcsI WCGNNNNNNNCGW 1 cut(s) 973
PfeI GAWTC 4 cut(s) 110, 442, 1688, 1798
PfoI TCCNGGA 1 cut(s) 162
PkrI GCNGC 4 cut(s) 466, 805, 808, 1361
PleI GAGTC 3 cut(s) 122, 179, 849
PpsI GAGTC 3 cut(s) 122, 179, 849
Ppu21I YACGTR 1 cut(s) 608
PpuMI RGGWCCY 1 cut(s) 1163
PscI ACATGT 1 cut(s) 859
PshAI GACNNNNGTC 1 cut(s) 1050
Psp5II RGGWCCY 1 cut(s) 1163
Psp6I CCWGG 2 cut(s) 637, 1027
PspGI CCWGG 2 cut(s) 637, 1027
PspN4I GGNNCC 5 cut(s) 41, 421, 663, 880, 1164
PspPI GGNCC 2 cut(s) 710, 1163
PspPPI RGGWCCY 1 cut(s) 1163
PsrI GAACNNNNNNTAC 2 cut(s) 580, 612
PstI CTGCAG 2 cut(s) 1519, 1609
PsuI RGATCY 2 cut(s) 1183, 1391
RsaI GTAC 7 cut(s) 32, 507, 764, 835, 1349, 1814, 1820
RsaNI GTAC 7 cut(s) 31, 506, 763, 834, 1348, 1813, 1819
RseI CAYNNNNRTG 3 cut(s) 236, 1431, 1665
SaqAI TTAA 6 cut(s) 192, 393, 567, 947, 1709, 1844
SatI GCNGC 4 cut(s) 465, 804, 807, 1360
Sau3AI GATC 9 cut(s) 379, 610, 784, 1183, 1290, 1315, 1391, 1436, 1553
Sau96I GGNCC 2 cut(s) 710, 1163
SchI GAGTC 3 cut(s) 122, 179, 850
ScrFI CCNGG 3 cut(s) 164, 639, 1029
SduI GDGCHC 1 cut(s) 1532
SfaNI GCATC 5 cut(s) 33, 144, 1212, 1753, 1898
SfcI CTRYAG 3 cut(s) 1515, 1584, 1605
SinI GGWCC 2 cut(s) 710, 1163
SmiMI CAYNNNNRTG 3 cut(s) 236, 1431, 1665
SmlI CTYRAG 1 cut(s) 1311
SmoI CTYRAG 1 cut(s) 1311
SsiI CCGC 4 cut(s) 107, 1181, 1264, 1887
SspMI CTAG 3 cut(s) 363, 585, 1751
StyD4I CCNGG 3 cut(s) 162, 637, 1027
TaaI ACNGT 2 cut(s) 977, 1540
TaiI ACGT 3 cut(s) 150, 610, 1916
TaqI TCGA 2 cut(s) 501, 1429
TatI WGTACW 4 cut(s) 762, 833, 1347, 1818
TfiI GAWTC 4 cut(s) 110, 442, 1688, 1798
Tru1I TTAA 6 cut(s) 192, 393, 567, 947, 1709, 1844
Tru9I TTAA 6 cut(s) 192, 393, 567, 947, 1709, 1844
TscAI CASTG 4 cut(s) 50, 1325, 1543, 1581
TseFI GTSAC 2 cut(s) 934, 1043
TseI GCWGC 4 cut(s) 464, 803, 806, 1359
Tsp45I GTSAC 2 cut(s) 934, 1043
TspDTI ATGAA 9 cut(s) 299, 439, 634, 769, 1004, 1023, 1284, 1415, 1584
TspGWI ACGGA 4 cut(s) 415, 982, 1031, 1101
TspRI CASTG 4 cut(s) 50, 1325, 1543, 1581
VpaK11BI GGWCC 2 cut(s) 710, 1163
XapI RAATTY 3 cut(s) 563, 950, 1882
XceI RCATGY 2 cut(s) 54, 863
XspI CTAG 3 cut(s) 363, 585, 1751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.