Rmu_sc0005969.1_g000038

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005969.1
OG0001442
Physical Location & Seq
Reverse (-)
136181 .. 139221
3041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005969.1_g000038.1.cds

Sequence Viewer

Length: 1335 bp
atgagctgttcaaacttacgtgatcttggtacgttggttactggaaatcctggcttcattgactacagagtggctcctctgtcaaaatttgggaactactcatgtcaaaacacaagtggaggaccaaggataactcttaggtgcaatagttgtcaaccaattcaagacaatatgtatatttgctggcagttcattgatcttcctgataatcctgctgctgctgttggatttcagttcaatcttactacaaggagccatgctaataaaagacatctgagttttgttagtggaaccctgaagaatgcaagtactttggatgatagaccagttacattcagagggattaatacaaatatattgaaattcagtttatttccgcggatataccgtaatctacatgatctaaggctcatccaacctttgtttcatgcgtttgtaccgggttcattctttaatgacacaagtcagctccaagcatcccttcaaagtccgaaggatggaaaacttaacactacattgtacatcaacttcctctctgactacattgtggagatagataagcaaaatattatgggtcctgtgagcttccttgcggatcttggcggcttgtattgcatctccattggcatatttttctaccttctggtgcaatgtgagtttaggattaaaaaactccggaatgaagatagtgtgctgcgcacgatcagaaatcggcggaaagctcaagaccattgggataaattgaggaaatatgtgatgtacacatggaactgcaaagcattggatgaatgttatgagagcacaaacaacagatcattttgcactgttttcagtacacaaccagtactccgaagtgggacatcacagaagcgaatgcaacatgaagcagataccattagttttaacaggaaactcagcttaaataacaagaagactgtcgttcaagagtgcttggacactgatggggatgtggaattttatacatcgagaactaaagtgaaccctccggaaagttcatcaaattctggagctggagttggacctcatctgcaaagtgaattacttgcctctacaaggaatggaaaacagcaaggtgttgcttcgtgcgagcaagattcgtacctgcctaaagcatcatctctcacaaatgatgatatcattcctccacccccttcacttgaactgaaggaccctgctgaaatggatatgtctgatgttcagaagaatcttaaaagtttgtacgagtacaatgcgatgcttagggaaaaactagtagaggctcagtctttcttccattctttagctacagactcttcaacagaagtccaaacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

444

Amino Acids

50.16

Weight (kDa)

8.35

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 698
Acc36I ACCTGC 1 cut(s) 1131
AccII CGCG 1 cut(s) 379
AccIII TCCGGA 2 cut(s) 675, 1006
AciI CCGC 5 cut(s) 377, 379, 593, 603, 715
AclWI GGATC 1 cut(s) 603
AcsI RAATTY 4 cut(s) 86, 362, 974, 1021
AcuI CTGAAG 2 cut(s) 317, 1205
AfiI CCNNNNNNNGG 2 cut(s) 497, 1074
AgsI TTSAA 8 cut(s) 12, 164, 238, 361, 485, 944, 1181, 1317
AhlI ACTAGT 1 cut(s) 1270
AjnI CCWGG 1 cut(s) 49
AjuI GAANNNNNNNTTGG 4 cut(s) 408, 440, 465, 497
AluBI AGCT 7 cut(s) 6, 469, 585, 722, 918, 1031, 1304
AluI AGCT 7 cut(s) 6, 469, 585, 722, 918, 1031, 1304
Alw21I GWGCWC 1 cut(s) 803
AlwI GGATC 1 cut(s) 603
Aor13HI TCCGGA 2 cut(s) 675, 1006
ApeKI GCWGC 3 cut(s) 215, 218, 694
ApoI RAATTY 4 cut(s) 86, 362, 974, 1021
AseI ATTAAT 1 cut(s) 345
AspLEI GCGC 1 cut(s) 699
AspS9I GGNCC 4 cut(s) 122, 575, 1040, 1189
AsuC2I CCSGG 1 cut(s) 441
AvaII GGWCC 4 cut(s) 122, 575, 1040, 1189
BaeI ACNNNNGTAYC 2 cut(s) 21, 54
BarI GAAGNNNNNNTAC 2 cut(s) 512, 544
BbsI GAAGAC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 803
BbvI GCAGC 3 cut(s) 202, 205, 681
BccI CCATC 2 cut(s) 491, 956
BcgI CGANNNNNNTGC 2 cut(s) 1232, 1266
BciT130I CCWGG 1 cut(s) 51
BcnI CCSGG 1 cut(s) 441
BcuI ACTAGT 1 cut(s) 1270
BfaI CTAG 1 cut(s) 1271
BfmI CTRYAG 2 cut(s) 64, 1305
BfuAI ACCTGC 1 cut(s) 1131
BisI GCNGC 4 cut(s) 216, 219, 604, 695
BlsI GCNGC 4 cut(s) 217, 220, 605, 696
BmcAI AGTACT 2 cut(s) 310, 846
Bme1390I CCNGG 2 cut(s) 51, 441
Bme18I GGWCC 4 cut(s) 122, 575, 1040, 1189
BmgT120I GGNCC 4 cut(s) 122, 575, 1040, 1189
BmiI GGNNCC 5 cut(s) 75, 254, 292, 576, 1191
BmrFI CCNGG 2 cut(s) 51, 441
BmsI GCATC 4 cut(s) 485, 624, 1142, 1245
BoxI GACNNNNGTC 1 cut(s) 462
BpiI GAAGAC 1 cut(s) 938
BpmI CTGGAG 2 cut(s) 1047, 1053
Bpu10I CCTNAGC 1 cut(s) 1259
BpuEI CTTGAG 1 cut(s) 708
BpuMI CCSGG 1 cut(s) 441
BsaAI YACGTR 1 cut(s) 20
BsaJI CCNNGG 2 cut(s) 125, 377
BsaWI WCCGGW 2 cut(s) 675, 1006
BsaXI ACNNNNNCTCC 4 cut(s) 831, 861, 1020, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 1074
Bse1I ACTGG 3 cut(s) 46, 326, 842
Bse3DI GCAATG 1 cut(s) 656
BseAI TCCGGA 2 cut(s) 675, 1006
BseBI CCWGG 1 cut(s) 51
BseDI CCNNGG 2 cut(s) 125, 377
BseGI GGATG 6 cut(s) 322, 411, 476, 502, 790, 973
BseLI CCNNNNNNNGG 2 cut(s) 497, 1074
BseMI GCAATG 1 cut(s) 656
BseMII CTCAG 3 cut(s) 266, 928, 1295
BseNI ACTGG 3 cut(s) 46, 326, 842
BseRI GAGGAG 1 cut(s) 66
BseXI GCAGC 3 cut(s) 202, 205, 681
Bsh1236I CGCG 1 cut(s) 379
BsiHKAI GWGCWC 1 cut(s) 803
BsiSI CCGG 3 cut(s) 440, 676, 1007
BslFI GGGAC 1 cut(s) 871
BslI CCNNNNNNNGG 2 cut(s) 497, 1074
BsmFI GGGAC 1 cut(s) 871
BsmI GAATGC 2 cut(s) 307, 879
Bsp1286I GDGCHC 1 cut(s) 803
Bsp13I TCCGGA 2 cut(s) 675, 1006
Bsp1407I TGTACA 2 cut(s) 519, 759
Bsp143I GATC 6 cut(s) 22, 196, 400, 595, 702, 812
BspACI CCGC 5 cut(s) 377, 379, 593, 603, 715
BspCNI CTCAG 3 cut(s) 267, 927, 1294
BspEI TCCGGA 2 cut(s) 675, 1006
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 5 cut(s) 75, 254, 292, 576, 1191
BspMI ACCTGC 1 cut(s) 1131
BspPI GGATC 1 cut(s) 603
BsrDI GCAATG 1 cut(s) 656
BsrGI TGTACA 2 cut(s) 519, 759
BsrI ACTGG 3 cut(s) 46, 326, 842
BssECI CCNNGG 2 cut(s) 125, 377
BssMI GATC 6 cut(s) 22, 196, 400, 595, 702, 812
BssT1I CCWWGG 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 389, 826, 937
Bst6I CTCTTC 1 cut(s) 1318
BstAUI TGTACA 2 cut(s) 519, 759
BstBAI YACGTR 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 185, 1109
BstDEI CTNAG 6 cut(s) 137, 275, 404, 914, 1259, 1281
BstDSI CCRYGG 1 cut(s) 377
BstENI CCTNNNNNAGG 1 cut(s) 1072
BstF5I GGATG 6 cut(s) 322, 411, 476, 502, 790, 973
BstFNI CGCG 1 cut(s) 379
BstHHI GCGC 1 cut(s) 699
BstKTI GATC 6 cut(s) 25, 199, 403, 598, 705, 815
BstMBI GATC 6 cut(s) 22, 196, 400, 595, 702, 812
BstMWI GCNNNNNNNGC 1 cut(s) 612
BstNI CCWGG 1 cut(s) 51
BstPAI GACNNNNGTC 1 cut(s) 462
BstSCI CCNGG 2 cut(s) 49, 439
BstSFI CTRYAG 2 cut(s) 64, 1305
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 3 cut(s) 202, 205, 681
BstV2I GAAGAC 1 cut(s) 938
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 377
BtgZI GCGATG 1 cut(s) 1268
BtsCI GGATG 6 cut(s) 322, 411, 476, 502, 790, 973
BtsIMutI CAGTG 2 cut(s) 822, 957
BveI ACCTGC 1 cut(s) 1131
Cac8I GCNNGC 2 cut(s) 185, 1109
CfoI GCGC 1 cut(s) 699
Cfr13I GGNCC 4 cut(s) 122, 575, 1040, 1189
Cfr42I CCGCGG 1 cut(s) 380
CviAII CATG 6 cut(s) 102, 257, 398, 428, 765, 881
DdeI CTNAG 6 cut(s) 137, 275, 404, 914, 1259, 1281
DpnI GATC 6 cut(s) 24, 198, 402, 597, 704, 814
DpnII GATC 6 cut(s) 22, 196, 400, 595, 702, 812
Eam1104I CTCTTC 1 cut(s) 1318
EarI CTCTTC 1 cut(s) 1318
EciI GGCGGA 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 125
Eco32I GATATC 1 cut(s) 1156
Eco47I GGWCC 4 cut(s) 122, 575, 1040, 1189
Eco57I CTGAAG 2 cut(s) 317, 1205
EcoNI CCTNNNNNAGG 1 cut(s) 1072
EcoO109I RGGNCCY 2 cut(s) 575, 1189
EcoRII CCWGG 1 cut(s) 49
EcoRV GATATC 1 cut(s) 1156
EcoT14I CCWWGG 1 cut(s) 125
ErhI CCWWGG 1 cut(s) 125
FaeI CATG 6 cut(s) 105, 260, 401, 431, 768, 884
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 1 cut(s) 871
FatI CATG 6 cut(s) 101, 256, 397, 427, 764, 880
Fnu4HI GCNGC 4 cut(s) 216, 219, 604, 695
FokI GGATG 6 cut(s) 329, 398, 463, 509, 797, 980
Fsp4HI GCNGC 4 cut(s) 216, 219, 604, 695
FspBI CTAG 1 cut(s) 1271
FspI TGCGCA 1 cut(s) 698
GlaI GCGC 1 cut(s) 698
GluI GCNGC 4 cut(s) 216, 219, 604, 695
GsuI CTGGAG 2 cut(s) 1047, 1053
HapII CCGG 3 cut(s) 440, 676, 1007
HhaI GCGC 1 cut(s) 699
Hin1II CATG 6 cut(s) 105, 260, 401, 431, 768, 884
Hin6I GCGC 1 cut(s) 697
HinP1I GCGC 1 cut(s) 697
HincII GTYRAC 1 cut(s) 155
HindII GTYRAC 1 cut(s) 155
HinfI GANTC 3 cut(s) 1115, 1225, 1310
HpaII CCGG 3 cut(s) 440, 676, 1007
Hpy166II GTNNAC 4 cut(s) 155, 762, 836, 1000
Hpy188I TCNGA 8 cut(s) 276, 338, 492, 538, 707, 851, 1213, 1221
Hpy188III TCNNGA 8 cut(s) 164, 203, 676, 725, 944, 987, 1007, 1026
Hpy8I GTNNAC 4 cut(s) 155, 762, 836, 1000
HpyAV CCTTC 5 cut(s) 487, 491, 650, 1180, 1182
HpyCH4III ACNGT 3 cut(s) 389, 826, 937
HpyCH4IV ACGT 2 cut(s) 19, 32
HpyCH4V TGCA 8 cut(s) 144, 305, 615, 649, 774, 822, 877, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 612
HpyF3I CTNAG 6 cut(s) 137, 275, 404, 914, 1259, 1281
HpySE526I ACGT 2 cut(s) 19, 32
Hsp92II CATG 6 cut(s) 105, 260, 401, 431, 768, 884
HspAI GCGC 1 cut(s) 697
Kpn2I TCCGGA 2 cut(s) 675, 1006
KspI CCGCGG 1 cut(s) 380
Kzo9I GATC 6 cut(s) 22, 196, 400, 595, 702, 812
LmnI GCTCC 4 cut(s) 79, 252, 474, 1028
Lsp1109I GCAGC 3 cut(s) 202, 205, 681
LweI GCATC 4 cut(s) 485, 624, 1142, 1245
MaeI CTAG 1 cut(s) 1271
MaeII ACGT 2 cut(s) 19, 32
MaeIII GTNAC 2 cut(s) 37, 328
MalI GATC 6 cut(s) 24, 198, 402, 597, 704, 814
MboI GATC 6 cut(s) 22, 196, 400, 595, 702, 812
MboII GAAGA 7 cut(s) 191, 310, 695, 943, 1234, 1282, 1305
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 803
MluCI AATT 7 cut(s) 86, 159, 362, 740, 974, 1021, 1058
MlyI GAGTC 1 cut(s) 1304
MmeI TCCRAC 3 cut(s) 205, 439, 1018
MroI TCCGGA 2 cut(s) 675, 1006
MseI TTAA 7 cut(s) 345, 453, 507, 666, 903, 920, 1230
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 3 cut(s) 440, 676, 1007
MspR9I CCNGG 2 cut(s) 51, 441
Mva1269I GAATGC 2 cut(s) 307, 879
MvaI CCWGG 1 cut(s) 51
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 1 cut(s) 612
NciI CCSGG 1 cut(s) 441
NdeII GATC 6 cut(s) 22, 196, 400, 595, 702, 812
NlaIII CATG 6 cut(s) 105, 260, 401, 431, 768, 884
NlaIV GGNNCC 5 cut(s) 75, 254, 292, 576, 1191
NsbI TGCGCA 1 cut(s) 698
PctI GAATGC 2 cut(s) 307, 879
PfeI GAWTC 2 cut(s) 1115, 1225
PkrI GCNGC 4 cut(s) 217, 220, 605, 696
PleI GAGTC 1 cut(s) 1304
PpsI GAGTC 1 cut(s) 1304
Ppu21I YACGTR 1 cut(s) 20
PpuMI RGGWCCY 2 cut(s) 575, 1189
PshAI GACNNNNGTC 1 cut(s) 462
PshBI ATTAAT 1 cut(s) 345
Psp5II RGGWCCY 2 cut(s) 575, 1189
Psp6I CCWGG 1 cut(s) 49
PspGI CCWGG 1 cut(s) 49
PspN4I GGNNCC 5 cut(s) 75, 254, 292, 576, 1191
PspPI GGNCC 4 cut(s) 122, 575, 1040, 1189
PspPPI RGGWCCY 2 cut(s) 575, 1189
PsuI RGATCY 1 cut(s) 595
SacII CCGCGG 1 cut(s) 380
SaqAI TTAA 7 cut(s) 345, 453, 507, 666, 903, 920, 1230
SatI GCNGC 4 cut(s) 216, 219, 604, 695
Sau3AI GATC 6 cut(s) 22, 196, 400, 595, 702, 812
Sau96I GGNCC 4 cut(s) 122, 575, 1040, 1189
ScaI AGTACT 2 cut(s) 310, 846
SchI GAGTC 1 cut(s) 1304
ScrFI CCNGG 2 cut(s) 51, 441
SduI GDGCHC 1 cut(s) 803
SfaNI GCATC 4 cut(s) 485, 624, 1142, 1245
SfcI CTRYAG 2 cut(s) 64, 1305
Sfr303I CCGCGG 1 cut(s) 380
SgrBI CCGCGG 1 cut(s) 380
SinI GGWCC 4 cut(s) 122, 575, 1040, 1189
SmlI CTYRAG 1 cut(s) 723
SmoI CTYRAG 1 cut(s) 723
SpeI ACTAGT 1 cut(s) 1270
Sse9I AATT 7 cut(s) 86, 159, 362, 740, 974, 1021, 1058
SsiI CCGC 5 cut(s) 377, 379, 593, 603, 715
SspI AATATT 1 cut(s) 568
SspMI CTAG 1 cut(s) 1271
StyD4I CCNGG 2 cut(s) 49, 439
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 3 cut(s) 389, 826, 937
TaiI ACGT 2 cut(s) 22, 35
TaqI TCGA 1 cut(s) 986
TasI AATT 7 cut(s) 86, 159, 362, 740, 974, 1021, 1058
TatI WGTACW 6 cut(s) 308, 519, 759, 833, 844, 1245
TauI GCSGC 1 cut(s) 606
TfiI GAWTC 2 cut(s) 1115, 1225
Tru1I TTAA 7 cut(s) 345, 453, 507, 666, 903, 920, 1230
Tru9I TTAA 7 cut(s) 345, 453, 507, 666, 903, 920, 1230
TscAI CASTG 2 cut(s) 829, 964
TseI GCWGC 3 cut(s) 215, 218, 694
TspDTI ATGAA 8 cut(s) 46, 181, 416, 435, 696, 801, 897, 1005
TspRI CASTG 2 cut(s) 829, 964
VpaK11BI GGWCC 4 cut(s) 122, 575, 1040, 1189
VspI ATTAAT 1 cut(s) 345
XagI CCTNNNNNAGG 1 cut(s) 1072
XapI RAATTY 4 cut(s) 86, 362, 974, 1021
XspI CTAG 1 cut(s) 1271
ZrmI AGTACT 2 cut(s) 310, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.