MD04G1241700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001442
Physical Location & Seq
Forward (+)
31870338 .. 31872068
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1241700.v1.1.491

Sequence Viewer

Length: 780 bp
ATGATTTTCTTGCTACTACATCTTATGAGGTTCCTTGCGGATCTTGGTGGCCTGTATTGCATCTGCATTGGCATTTTTTTGTGCCTTCTGGTGCAATGTGAGTTCAGGATAAAAAAACTCCGCAATGAAGATATCGTGTTGCGGAGGATCAGAAATCGGCGAAAAGCTCAAGACCACTGGGATAAATTGAGGAAATATGTGATGTATACATGGGGCTGCAAGGCATTGGATGATTATTACGAGAACATAAACAAGGGATCAGGTTGCGTTAGTTTTGGTACACAGTCATTTCATCGAAATGGATCATCACATAAGCAAAGGCAACAAGAAACGGCGATTAGTTTTAACAGGAAGCTCAGCTTGCCTAAGAAGAAGACTCTGGTTCAAGAGTGCTCTGACACTGTTGGGAGTATGAAATCATTTACACCGGGAACCACACCGAACCCAGAAGAAAGTTCATCGCATTCAGCAGTCAGACCTCATATGCAAATGGAATCGCTTGGCTCTACAGAGGATGGAAAACAGCAATTTGTTCGTGCGTCTAAAGAAGATTCCTCCCCGCCTAAAGCCTTAACTCCGACAATCGATGATATCATTCCTCCACCACCGTCACTAGAACTGAAGGATGCTTCTGAAATGGATATGTCTGATGTACAAAAGAGTCTTAGAAGTTTGCACGAGTACAACGTTATGTTGAGGGAAAAATTAGTAGCTGCGCAGTCTTTGTTCCATTCTTCAGCTACAAAATCCTCATCTTCTGTAACAGAAACCCAAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.37

Weight (kDa)

9.02

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 717
AccI GTMKAC 1 cut(s) 206
AciI CCGC 4 cut(s) 38, 121, 142, 560
AclI AACGTT 1 cut(s) 687
AclWI GGATC 4 cut(s) 48, 155, 265, 310
AcuI CTGAAG 2 cut(s) 641, 720
AfaI GTAC 3 cut(s) 280, 654, 683
AgsI TTSAA 1 cut(s) 386
AluBI AGCT 5 cut(s) 167, 355, 360, 713, 740
AluI AGCT 5 cut(s) 167, 355, 360, 713, 740
Alw21I GWGCWC 1 cut(s) 395
AlwI GGATC 4 cut(s) 48, 155, 265, 310
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 2 cut(s) 216, 713
AspLEI GCGC 1 cut(s) 718
AsuC2I CCSGG 1 cut(s) 429
BauI CACGAG 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 380
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 2 cut(s) 203, 700
BccI CCATC 1 cut(s) 509
BceAI ACGGC 1 cut(s) 348
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 1 cut(s) 614
BfmI CTRYAG 1 cut(s) 507
BisI GCNGC 2 cut(s) 217, 714
BlpI GCTNAGC 1 cut(s) 356
BlsI GCNGC 2 cut(s) 218, 715
Bme1390I CCNGG 1 cut(s) 429
BmiI GGNNCC 2 cut(s) 32, 433
BmrFI CCNGG 1 cut(s) 429
BmrI ACTGGG 1 cut(s) 187
BmsI GCATC 2 cut(s) 69, 616
BmuI ACTGGG 1 cut(s) 187
BpiI GAAGAC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 356
BpuEI CTTGAG 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 429
Bsa29I ATCGAT 1 cut(s) 585
Bse1I ACTGG 1 cut(s) 182
Bse3DI GCAATG 2 cut(s) 101, 130
BseCI ATCGAT 1 cut(s) 585
BseGI GGATG 3 cut(s) 235, 520, 631
BseMI GCAATG 2 cut(s) 101, 130
BseMII CTCAG 1 cut(s) 370
BseNI ACTGG 1 cut(s) 182
BseXI GCAGC 2 cut(s) 203, 700
BshFI GGCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 585
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 1 cut(s) 428
BsmI GAATGC 1 cut(s) 463
BsnI GGCC 1 cut(s) 51
Bsp1286I GDGCHC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 4 cut(s) 40, 147, 257, 302
Bsp1720I GCTNAGC 1 cut(s) 356
BspACI CCGC 4 cut(s) 38, 121, 142, 560
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 1 cut(s) 369
BspDI ATCGAT 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 32, 433
BspPI GGATC 4 cut(s) 48, 155, 265, 310
BsrDI GCAATG 2 cut(s) 101, 130
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 1 cut(s) 182
BssMI GATC 4 cut(s) 40, 147, 257, 302
BssNAI GTATAC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 677
Bst1107I GTATAC 1 cut(s) 207
Bst2BI CACGAG 1 cut(s) 677
Bst4CI ACNGT 3 cut(s) 285, 403, 609
BstAUI TGTACA 1 cut(s) 652
BstC8I GCNNGC 1 cut(s) 362
BstDEI CTNAG 3 cut(s) 356, 366, 665
BstF5I GGATG 3 cut(s) 235, 520, 631
BstHHI GCGC 1 cut(s) 718
BstKTI GATC 4 cut(s) 43, 150, 260, 305
BstMBI GATC 4 cut(s) 40, 147, 257, 302
BstMWI GCNNNNNNNGC 2 cut(s) 57, 361
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 2 cut(s) 203, 700
BstV2I GAAGAC 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
BstZ17I GTATAC 1 cut(s) 207
Bsu15I ATCGAT 1 cut(s) 585
BsuRI GGCC 1 cut(s) 51
BsuTUI ATCGAT 1 cut(s) 585
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 3 cut(s) 235, 520, 631
BtsIMutI CAGTG 2 cut(s) 175, 399
Cac8I GCNNGC 1 cut(s) 362
CfoI GCGC 1 cut(s) 718
ClaI ATCGAT 1 cut(s) 585
CseI GACGC 1 cut(s) 528
Csp6I GTAC 3 cut(s) 279, 653, 682
CviAII CATG 1 cut(s) 210
CviJI RGCY 9 cut(s) 51, 167, 216, 355, 360, 504, 569, 713, 740
CviKI_1 RGCY 9 cut(s) 51, 167, 216, 355, 360, 504, 569, 713, 740
CviQI GTAC 3 cut(s) 279, 653, 682
DdeI CTNAG 3 cut(s) 356, 366, 665
DpnI GATC 4 cut(s) 42, 149, 259, 304
DpnII GATC 4 cut(s) 40, 147, 257, 302
Eco32I GATATC 2 cut(s) 133, 592
Eco57I CTGAAG 2 cut(s) 641, 720
EcoRV GATATC 2 cut(s) 133, 592
FaeI CATG 1 cut(s) 213
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 1 cut(s) 209
FauI CCCGC 1 cut(s) 567
FauNDI CATATG 1 cut(s) 483
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 2 cut(s) 217, 714
FokI GGATG 3 cut(s) 242, 527, 638
Fsp4HI GCNGC 2 cut(s) 217, 714
FspBI CTAG 1 cut(s) 614
FspI TGCGCA 1 cut(s) 717
GlaI GCGC 1 cut(s) 717
GluI GCNGC 2 cut(s) 217, 714
HaeIII GGCC 1 cut(s) 51
HapII CCGG 1 cut(s) 428
HgaI GACGC 1 cut(s) 528
HhaI GCGC 1 cut(s) 718
Hin1II CATG 1 cut(s) 213
Hin6I GCGC 1 cut(s) 716
HinP1I GCGC 1 cut(s) 716
HinfI GANTC 4 cut(s) 376, 494, 551, 661
HpaII CCGG 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 207, 281
Hpy188I TCNGA 6 cut(s) 152, 397, 476, 579, 634, 649
Hpy188III TCNNGA 3 cut(s) 106, 170, 386
Hpy8I GTNNAC 2 cut(s) 207, 281
HpyAV CCTTC 2 cut(s) 95, 616
HpyCH4III ACNGT 3 cut(s) 285, 403, 609
HpyCH4IV ACGT 2 cut(s) 687, 776
HpyCH4V TGCA 6 cut(s) 60, 66, 94, 219, 487, 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 361
HpyF3I CTNAG 3 cut(s) 356, 366, 665
HpySE526I ACGT 2 cut(s) 687, 776
Hsp92II CATG 1 cut(s) 213
HspAI GCGC 1 cut(s) 716
Kzo9I GATC 4 cut(s) 40, 147, 257, 302
LpnPI CCDG 9 cut(s) 65, 74, 91, 163, 246, 334, 365, 441, 459
Lsp1109I GCAGC 2 cut(s) 203, 700
LweI GCATC 2 cut(s) 69, 616
MaeI CTAG 1 cut(s) 614
MaeII ACGT 2 cut(s) 687, 776
MaeIII GTNAC 2 cut(s) 609, 760
MalI GATC 4 cut(s) 42, 149, 259, 304
MboI GATC 4 cut(s) 40, 147, 257, 302
MboII GAAGA 7 cut(s) 140, 382, 385, 461, 560, 726, 747
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 3 cut(s) 185, 527, 704
MlyI GAGTC 2 cut(s) 370, 670
MmeI TCCRAC 1 cut(s) 602
MnlI CCTC 9 cut(s) 21, 138, 183, 489, 505, 565, 609, 690, 760
MseI TTAA 2 cut(s) 345, 572
MslI CAYNNNNRTG 1 cut(s) 297
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 1 cut(s) 429
Mva1269I GAATGC 1 cut(s) 463
MwoI GCNNNNNNNGC 2 cut(s) 57, 361
NciI CCSGG 1 cut(s) 429
NdeI CATATG 1 cut(s) 483
NdeII GATC 4 cut(s) 40, 147, 257, 302
NlaIII CATG 1 cut(s) 213
NlaIV GGNNCC 2 cut(s) 32, 433
NmuCI GTSAC 1 cut(s) 609
NsbI TGCGCA 1 cut(s) 717
PcsI WCGNNNNNNNCGW 1 cut(s) 684
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 494, 551
PkrI GCNGC 2 cut(s) 218, 715
PleI GAGTC 2 cut(s) 370, 669
PpsI GAGTC 2 cut(s) 370, 669
Psp1406I AACGTT 1 cut(s) 687
PspN4I GGNNCC 2 cut(s) 32, 433
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 3 cut(s) 280, 654, 683
RsaNI GTAC 3 cut(s) 279, 653, 682
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 2 cut(s) 345, 572
SatI GCNGC 2 cut(s) 217, 714
Sau3AI GATC 4 cut(s) 40, 147, 257, 302
SchI GAGTC 2 cut(s) 370, 670
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 1 cut(s) 395
SfaNI GCATC 2 cut(s) 69, 616
SfcI CTRYAG 1 cut(s) 507
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 3 cut(s) 185, 527, 704
SsiI CCGC 4 cut(s) 38, 121, 142, 560
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 3 cut(s) 285, 403, 609
TaiI ACGT 2 cut(s) 690, 779
TaqI TCGA 2 cut(s) 295, 585
TasI AATT 3 cut(s) 185, 527, 704
TatI WGTACW 2 cut(s) 652, 681
TfiI GAWTC 2 cut(s) 494, 551
Tru1I TTAA 2 cut(s) 345, 572
Tru9I TTAA 2 cut(s) 345, 572
TscAI CASTG 2 cut(s) 182, 406
TseFI GTSAC 1 cut(s) 609
TseI GCWGC 2 cut(s) 216, 713
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 4 cut(s) 141, 281, 428, 447
TspRI CASTG 2 cut(s) 182, 406
XmiI GTMKAC 1 cut(s) 206
XspI CTAG 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.