MD12G1258400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001442
Physical Location & Seq
Forward (+)
32528057 .. 32532152
4096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1258400.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGCCATGTCCATCAAACAGCATCAGCTACAATGGCAGCCAGTGCGCCTGCGCAGTTGGGTACATTCTAAACCAAACCGCCGCCAGCTGCGTCCTGTTTGCCGCCAATTCCACCATCTCCACCGATGGCGGCATCAACAGCTACGCCTTAAACTTCCCGGAGACCTTCTTCTCCTTTGACTCCATCAGAAAGTTCACGCAATCGCAAGCCGTCTTCTTGGAGGCCACGCTTGTGTTGCTGCTTTCTTGGTTTTGCTGTTGCCTCTTCCTCAGGTTTATGAAGCTCGGCAACGACGGCCGGAGCATTTGGTTCCGTCTCCGGTGGTGGATTAGCCGATTGGATGTTTGCTTTGCCACTAGGCATTGGCTGGATGATCAAAAGGTAGTTAAGAAACGAAAAACGGAACTTGGTGGAATGTTCTCAATGGCAAGTTGGATTCTCTTCGTTGGCTTGTTTGCTGCGTTGCTCTACCAAACCATATCAAATAGAAGTATCGAGGTGCATAATGTCAGAGCAACAAATGCACCTGACTTGGCTGCCTTCAAGAATGACATGGAATTTAACGTAACCGCGGTTTCTAGTATGAGCTGTTCAAACTTACGTGATCTTGGTACTTTAGTTACTGGAAATCCTGGCTTCATTGACTACAGAGTCACTCCTCTGTCAAAATTCGGGAACTACTCGTGTCGGAATACGAGTATAGGACCAACTATAACTCTTAGGTGCAGCAATTGTCAACCACTTGAAGACAATTTGTACATTTCATGGCAGTTCGTCGATCTTCCTAATAATCCTGCTGCTGCTGTTGGATTTCAGTTCAATCTCAGTACAAGGAGTCATGCTAGTAAAAAATATGTCAGTTTTGTTAGCGGAACCCTAAAGAATGGAAGCAGTTTTGATGATACACCAGTTACATTCAGAGGAAATACTACAAATATATTAAAATTTAGTTTGTTTCCCCAGATATACCATAATCATCATGATCTAAGGCTCATCCAACCTCTGTTTCATGAGTTTGTTCCGGGTTCATTTTTCAGTGATGAAAGTCAGCTCCGATCGTCCCTTGAAAATTCTAAGGATGGAATGCTCAACACTACATTATATATCAACTTTCTCTCTGCCTATATCGTGGAGATAGATAACCAAAACGTTGCGGGTCCTGTGAGCTTCCTTGCGGATCTTGGTGGCCTGTATTGCATCTGTATTGGCATTTTTTTCTACCTTTTGGTGCAATTTGAGTTCAGGATAAAAAAACTCCGGAATGAAGATATCGTGTTGCGGAGGATCAGAAATCGGCGAAAAGCTCAAGACCGCTGGGATAAATTAAGGAAATATGTGATGTATACATGGGGCTGCAAGGCATTGGAGGATTATTACGAGAGCATAGACGAGGGATCAGGTTGTGTGAATTGTGGTACACAATCAGTTCATCGAAATGGATCATCACATAAGCGAAGGCAACAAGAAACAGTGATTAGTTTTAACAGGAAGCTCAGCTTGCCTAAGAAGACTCTGGTTCAAGAGTGCTCTGACACTGTTGGGAGTGTGAAGTCATTTACACTGGGAACTACACCGAACCCGGAAGAAAGTTCATCGCATTCAGCAGTCAGACCTCCTCTGCAAATGGAATCGCTTGGCTCTACAAAGGACGGAAAACAGCAATTTGTTCATGCGTCTAAAGAAGATTCCTGTCCACCTAAAGCCTTAACTCCTACAATTGGTGATATCATTCCTCCACCTCCTTCACTAGAATTGAAGGATGCTTCTGAAATGGATATGTCTGATGTTCAAAAGAATCTTAGAAGTTTGCACGAGTACAACGTTTTGTTGAGGGAAAAGTTAGTAGCTACGCAGTCTTTGTTCCGTTCTTTAGCTACAAAATCAACAGAAAGCCAAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

634

Amino Acids

71.24

Weight (kDa)

8.72

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
Acc16I TGCGCA 1 cut(s) 52
AccI GTMKAC 1 cut(s) 1343
AccII CGCG 1 cut(s) 572
AccIII TCCGGA 1 cut(s) 1257
AclI AACGTT 2 cut(s) 1149, 1821
AclWI GGATC 4 cut(s) 1185, 1292, 1402, 1447
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 4 cut(s) 557, 668, 944, 1069
AfaI GTAC 6 cut(s) 62, 613, 758, 829, 1417, 1817
AfiI CCNNNNNNNGG 1 cut(s) 1718
AgsI TTSAA 8 cut(s) 544, 594, 746, 820, 1067, 1520, 1756, 1790
AjnI CCWGG 1 cut(s) 631
AjuI GAANNNNNNNTTGG 2 cut(s) 990, 1022
AleI CACNNNNGTG 1 cut(s) 230
Alw21I GWGCWC 1 cut(s) 1529
Alw26I GTCTC 2 cut(s) 155, 320
AlwI GGATC 4 cut(s) 1185, 1292, 1402, 1447
Aor13HI TCCGGA 1 cut(s) 1257
AoxI GGCC 3 cut(s) 222, 295, 1186
ApeKI GCWGC 9 cut(s) 36, 87, 238, 458, 536, 726, 797, 800, 1353
ApoI RAATTY 4 cut(s) 557, 668, 944, 1069
AspLEI GCGC 2 cut(s) 47, 53
AspS9I GGNCC 2 cut(s) 704, 1157
AsuC2I CCSGG 3 cut(s) 158, 1023, 1580
AsuHPI GGTGA 1 cut(s) 1733
AvaII GGWCC 2 cut(s) 704, 1157
AxyI CCTNAGG 1 cut(s) 269
BaeI ACNNNNGTAYC 4 cut(s) 603, 636, 894, 927
BauI CACGAG 2 cut(s) 682, 1811
BbsI GAAGAC 3 cut(s) 205, 753, 1514
Bbv12I GWGCWC 1 cut(s) 1529
BbvI GCAGC 9 cut(s) 48, 74, 225, 445, 523, 738, 784, 787, 1340
BccI CCATC 5 cut(s) 19, 119, 122, 191, 1073
BceAI ACGGC 2 cut(s) 194, 310
BciT130I CCWGG 1 cut(s) 633
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 3 cut(s) 158, 1023, 1580
BcoDI GTCTC 2 cut(s) 155, 320
BfaI CTAG 4 cut(s) 357, 579, 843, 1748
BfmI CTRYAG 1 cut(s) 646
BlpI GCTNAGC 1 cut(s) 1493
Bme1390I CCNGG 4 cut(s) 158, 633, 1023, 1580
Bme18I GGWCC 2 cut(s) 704, 1157
BmgT120I GGNCC 2 cut(s) 704, 1157
BmiI GGNNCC 3 cut(s) 311, 874, 1158
BmrFI CCNGG 4 cut(s) 158, 633, 1023, 1580
BmrI ACTGGG 1 cut(s) 1571
BmsI GCATC 4 cut(s) 30, 141, 1206, 1750
BmuI ACTGGG 1 cut(s) 1571
BpiI GAAGAC 3 cut(s) 205, 753, 1514
Bpu1102I GCTNAGC 1 cut(s) 1493
BpuEI CTTGAG 1 cut(s) 1290
BpuMI CCSGG 3 cut(s) 158, 1023, 1580
BsaAI YACGTR 1 cut(s) 602
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 1 cut(s) 570
BsaWI WCCGGW 2 cut(s) 318, 1257
Bsc4I CCNNNNNNNGG 1 cut(s) 1718
Bse1I ACTGG 4 cut(s) 40, 628, 908, 1566
Bse21I CCTNAGG 1 cut(s) 269
BseAI TCCGGA 1 cut(s) 1257
BseBI CCWGG 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 5 cut(s) 346, 376, 993, 1084, 1765
BseLI CCNNNNNNNGG 1 cut(s) 1718
BseMII CTCAG 3 cut(s) 283, 838, 1507
BseNI ACTGG 4 cut(s) 40, 628, 908, 1566
BseRI GAGGAG 2 cut(s) 648, 1605
BseX3I CGGCCG 1 cut(s) 295
BseXI GCAGC 9 cut(s) 48, 74, 225, 445, 523, 738, 784, 787, 1340
BseYI CCCAGC 1 cut(s) 1314
BsgI GTGCAG 1 cut(s) 745
Bsh1236I CGCG 1 cut(s) 572
Bsh1285I CGRYCG 2 cut(s) 298, 1058
BshFI GGCC 3 cut(s) 224, 297, 1188
BsiEI CGRYCG 2 cut(s) 298, 1058
BsiHKAI GWGCWC 1 cut(s) 1529
BsiSI CCGG 6 cut(s) 158, 298, 319, 1022, 1258, 1580
BslFI GGGAC 1 cut(s) 1045
BslI CCNNNNNNNGG 1 cut(s) 1718
BsmAI GTCTC 2 cut(s) 155, 320
BsmBI CGTCTC 1 cut(s) 320
BsmFI GGGAC 1 cut(s) 1045
BsmI GAATGC 2 cut(s) 1089, 1597
BsnI GGCC 3 cut(s) 224, 297, 1188
Bso31I GGTCTC 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 1529
Bsp13I TCCGGA 1 cut(s) 1257
Bsp1407I TGTACA 1 cut(s) 756
Bsp143I GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
Bsp1720I GCTNAGC 1 cut(s) 1493
BspANI GGCC 3 cut(s) 224, 297, 1188
BspCNI CTCAG 3 cut(s) 282, 837, 1506
BspEI TCCGGA 1 cut(s) 1257
BspFNI CGCG 1 cut(s) 572
BspHI TCATGA 2 cut(s) 979, 1009
BspLI GGNNCC 3 cut(s) 311, 874, 1158
BspPI GGATC 4 cut(s) 1185, 1292, 1402, 1447
BspTNI GGTCTC 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 4 cut(s) 40, 628, 908, 1566
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
BssNAI GTATAC 1 cut(s) 1344
BssSI CACGAG 2 cut(s) 682, 1811
Bst1107I GTATAC 1 cut(s) 1344
Bst2BI CACGAG 2 cut(s) 682, 1811
Bst2UI CCWGG 1 cut(s) 633
Bst4CI ACNGT 2 cut(s) 1471, 1537
Bst6I CTCTTC 2 cut(s) 269, 446
BstAPI GCANNNNNTGC 2 cut(s) 42, 521
BstAUI TGTACA 1 cut(s) 756
BstBAI YACGTR 1 cut(s) 602
BstC8I GCNNGC 4 cut(s) 49, 85, 207, 1499
BstDEI CTNAG 8 cut(s) 269, 719, 824, 986, 1074, 1493, 1503, 1799
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 5 cut(s) 346, 376, 993, 1084, 1765
BstFNI CGCG 1 cut(s) 572
BstHHI GCGC 2 cut(s) 47, 53
BstKTI GATC 9 cut(s) 376, 607, 781, 985, 1058, 1180, 1287, 1397, 1442
BstMAI GTCTC 2 cut(s) 155, 320
BstMBI GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
BstMCI CGRYCG 2 cut(s) 298, 1058
BstMWI GCNNNNNNNGC 8 cut(s) 33, 42, 138, 235, 294, 521, 1194, 1498
BstNI CCWGG 1 cut(s) 633
BstSCI CCNGG 4 cut(s) 156, 631, 1021, 1578
BstSFI CTRYAG 1 cut(s) 646
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 9 cut(s) 48, 74, 225, 445, 523, 738, 784, 787, 1340
BstV2I GAAGAC 3 cut(s) 205, 753, 1514
BstX2I RGATCY 1 cut(s) 1177
BstYI RGATCY 1 cut(s) 1177
BstZ17I GTATAC 1 cut(s) 1344
BstZI CGGCCG 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 269
BsuRI GGCC 3 cut(s) 224, 297, 1188
BtgI CCRYGG 1 cut(s) 570
BtgZI GCGATG 1 cut(s) 1578
BtsCI GGATG 5 cut(s) 346, 376, 993, 1084, 1765
BtsIMutI CAGTG 5 cut(s) 47, 1042, 1476, 1533, 1559
Cac8I GCNNGC 4 cut(s) 49, 85, 207, 1499
CciI TCATGA 2 cut(s) 979, 1009
CfoI GCGC 2 cut(s) 47, 53
Cfr13I GGNCC 2 cut(s) 704, 1157
Cfr42I CCGCGG 1 cut(s) 573
CseI GACGC 2 cut(s) 79, 1662
Csp6I GTAC 6 cut(s) 61, 612, 757, 828, 1416, 1816
CviAII CATG 8 cut(s) 6, 553, 765, 839, 980, 1010, 1347, 1670
CviQI GTAC 6 cut(s) 61, 612, 757, 828, 1416, 1816
DdeI CTNAG 8 cut(s) 269, 719, 824, 986, 1074, 1493, 1503, 1799
DpnI GATC 9 cut(s) 375, 606, 780, 984, 1057, 1179, 1286, 1396, 1441
DpnII GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 1 cut(s) 295
EagI CGGCCG 1 cut(s) 295
Eam1104I CTCTTC 2 cut(s) 269, 446
EarI CTCTTC 2 cut(s) 269, 446
EclXI CGGCCG 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 155
Eco32I GATATC 2 cut(s) 1270, 1726
Eco47I GGWCC 2 cut(s) 704, 1157
Eco52I CGGCCG 1 cut(s) 295
Eco81I CCTNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 1157
EcoRII CCWGG 1 cut(s) 631
EcoRV GATATC 2 cut(s) 1270, 1726
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 8 cut(s) 9, 556, 768, 842, 983, 1013, 1350, 1673
FalI AAGNNNNNCTT 2 cut(s) 1481, 1513
FaqI GGGAC 1 cut(s) 1045
FatI CATG 8 cut(s) 5, 552, 764, 838, 979, 1009, 1346, 1669
FauI CCCGC 1 cut(s) 1147
FbaI TGATCA 1 cut(s) 373
FblI GTMKAC 1 cut(s) 1343
FokI GGATG 5 cut(s) 353, 383, 980, 1091, 1772
FspBI CTAG 4 cut(s) 357, 579, 843, 1748
FspI TGCGCA 1 cut(s) 52
GlaI GCGC 2 cut(s) 46, 52
GsaI CCCAGC 1 cut(s) 1318
HaeIII GGCC 3 cut(s) 224, 297, 1188
HapII CCGG 6 cut(s) 158, 298, 319, 1022, 1258, 1580
HgaI GACGC 2 cut(s) 79, 1662
HhaI GCGC 2 cut(s) 47, 53
Hin1II CATG 8 cut(s) 9, 556, 768, 842, 983, 1013, 1350, 1673
Hin6I GCGC 2 cut(s) 45, 51
HinP1I GCGC 2 cut(s) 45, 51
HincII GTYRAC 1 cut(s) 737
HindII GTYRAC 1 cut(s) 737
HinfI GANTC 8 cut(s) 179, 436, 651, 835, 1510, 1628, 1685, 1795
HpaII CCGG 6 cut(s) 158, 298, 319, 1022, 1258, 1580
HphI GGTGA 1 cut(s) 1733
Hpy166II GTNNAC 5 cut(s) 195, 737, 1344, 1418, 1694
Hpy188III TCNNGA 8 cut(s) 544, 673, 980, 1010, 1243, 1258, 1307, 1520
Hpy8I GTNNAC 5 cut(s) 195, 737, 1344, 1418, 1694
Hpy99I CGWCG 2 cut(s) 296, 779
HpyAV CCTTC 5 cut(s) 175, 550, 1449, 1750, 1752
HpyCH4III ACNGT 2 cut(s) 1471, 1537
HpyCH4IV ACGT 5 cut(s) 564, 601, 1149, 1821, 1898
HpyCH4V TGCA 8 cut(s) 502, 524, 726, 1197, 1231, 1356, 1621, 1810
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 42, 138, 235, 294, 521, 1194, 1498
HpyF3I CTNAG 8 cut(s) 269, 719, 824, 986, 1074, 1493, 1503, 1799
HpySE526I ACGT 5 cut(s) 564, 601, 1149, 1821, 1898
Hsp92II CATG 8 cut(s) 9, 556, 768, 842, 983, 1013, 1350, 1673
HspAI GCGC 2 cut(s) 45, 51
Kpn2I TCCGGA 1 cut(s) 1257
Ksp22I TGATCA 1 cut(s) 373
KspI CCGCGG 1 cut(s) 573
Kzo9I GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
LmnI GCTCC 2 cut(s) 300, 1056
Lsp1109I GCAGC 9 cut(s) 48, 74, 225, 445, 523, 738, 784, 787, 1340
LweI GCATC 4 cut(s) 30, 141, 1206, 1750
MaeI CTAG 4 cut(s) 357, 579, 843, 1748
MaeII ACGT 5 cut(s) 564, 601, 1149, 1821, 1898
MaeIII GTNAC 4 cut(s) 565, 619, 652, 910
MalI GATC 9 cut(s) 375, 606, 780, 984, 1057, 1179, 1286, 1396, 1441
MboI GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
MfeI CAATTG 2 cut(s) 730, 1716
MflI RGATCY 1 cut(s) 1177
MhlI GDGCHC 1 cut(s) 1529
MlyI GAGTC 4 cut(s) 173, 660, 844, 1504
MmeI TCCRAC 4 cut(s) 413, 668, 787, 1021
MroI TCCGGA 1 cut(s) 1257
MseI TTAA 7 cut(s) 149, 387, 561, 941, 1325, 1482, 1706
MslI CAYNNNNRTG 2 cut(s) 230, 1434
MspA1I CMGCKG 3 cut(s) 87, 572, 1314
MspI CCGG 6 cut(s) 158, 298, 319, 1022, 1258, 1580
MspR9I CCNGG 4 cut(s) 158, 633, 1023, 1580
MunI CAATTG 2 cut(s) 730, 1716
Mva1269I GAATGC 2 cut(s) 1089, 1597
MvaI CCWGG 1 cut(s) 633
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 8 cut(s) 33, 42, 138, 235, 294, 521, 1194, 1498
NciI CCSGG 3 cut(s) 158, 1023, 1580
NdeII GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
NlaIII CATG 8 cut(s) 9, 556, 768, 842, 983, 1013, 1350, 1673
NlaIV GGNNCC 3 cut(s) 311, 874, 1158
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 1 cut(s) 652
NsbI TGCGCA 1 cut(s) 52
OliI CACNNNNGTG 1 cut(s) 230
PagI TCATGA 2 cut(s) 979, 1009
PcsI WCGNNNNNNNCGW 1 cut(s) 1818
PctI GAATGC 2 cut(s) 1089, 1597
PfeI GAWTC 4 cut(s) 436, 1628, 1685, 1795
PfoI TCCNGGA 1 cut(s) 156
Ple19I CGATCG 1 cut(s) 1058
PleI GAGTC 4 cut(s) 173, 659, 843, 1504
PpsI GAGTC 4 cut(s) 173, 659, 843, 1504
Ppu21I YACGTR 1 cut(s) 602
PpuMI RGGWCCY 1 cut(s) 1157
Psp1406I AACGTT 2 cut(s) 1149, 1821
Psp5II RGGWCCY 1 cut(s) 1157
Psp6I CCWGG 1 cut(s) 631
PspFI CCCAGC 1 cut(s) 1314
PspGI CCWGG 1 cut(s) 631
PspN4I GGNNCC 3 cut(s) 311, 874, 1158
PspPI GGNCC 2 cut(s) 704, 1157
PspPPI RGGWCCY 1 cut(s) 1157
PsrI GAACNNNNNNTAC 2 cut(s) 574, 606
PsuI RGATCY 1 cut(s) 1177
PvuI CGATCG 1 cut(s) 1058
PvuII CAGCTG 1 cut(s) 87
RsaI GTAC 6 cut(s) 62, 613, 758, 829, 1417, 1817
RsaNI GTAC 6 cut(s) 61, 612, 757, 828, 1416, 1816
RseI CAYNNNNRTG 2 cut(s) 230, 1434
SacII CCGCGG 1 cut(s) 573
SaqAI TTAA 7 cut(s) 149, 387, 561, 941, 1325, 1482, 1706
Sau3AI GATC 9 cut(s) 373, 604, 778, 982, 1055, 1177, 1284, 1394, 1439
Sau96I GGNCC 2 cut(s) 704, 1157
SchI GAGTC 4 cut(s) 173, 660, 844, 1504
ScrFI CCNGG 4 cut(s) 158, 633, 1023, 1580
SduI GDGCHC 1 cut(s) 1529
SfaNI GCATC 4 cut(s) 30, 141, 1206, 1750
SfcI CTRYAG 1 cut(s) 646
Sfr303I CCGCGG 1 cut(s) 573
SgrBI CCGCGG 1 cut(s) 573
SinI GGWCC 2 cut(s) 704, 1157
SmiMI CAYNNNNRTG 2 cut(s) 230, 1434
SmlI CTYRAG 1 cut(s) 1305
SmoI CTYRAG 1 cut(s) 1305
SspMI CTAG 4 cut(s) 357, 579, 843, 1748
StyD4I CCNGG 4 cut(s) 156, 631, 1021, 1578
TaaI ACNGT 2 cut(s) 1471, 1537
TaiI ACGT 5 cut(s) 567, 604, 1152, 1824, 1901
TaqI TCGA 3 cut(s) 495, 777, 1432
TatI WGTACW 3 cut(s) 756, 827, 1815
TauI GCSGC 3 cut(s) 83, 104, 132
TfiI GAWTC 4 cut(s) 436, 1628, 1685, 1795
Tru1I TTAA 7 cut(s) 149, 387, 561, 941, 1325, 1482, 1706
Tru9I TTAA 7 cut(s) 149, 387, 561, 941, 1325, 1482, 1706
TscAI CASTG 5 cut(s) 47, 1042, 1476, 1540, 1566
TseFI GTSAC 1 cut(s) 652
TseI GCWGC 9 cut(s) 36, 87, 238, 458, 536, 726, 797, 800, 1353
Tsp45I GTSAC 1 cut(s) 652
TspGWI ACGGA 4 cut(s) 302, 416, 1665, 1853
TspRI CASTG 5 cut(s) 47, 1042, 1476, 1540, 1566
VpaK11BI GGWCC 2 cut(s) 704, 1157
XapI RAATTY 4 cut(s) 557, 668, 944, 1069
XmiI GTMKAC 1 cut(s) 1343
XspI CTAG 4 cut(s) 357, 579, 843, 1748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.