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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 3685 |
| Acc36I | ACCTGC | 5 cut(s) | 274, 358, 3029, 3685, 3778 |
| Acc65I | GGTACC | 1 cut(s) | 1787 |
| AccB1I | GGYRCC | 1 cut(s) | 1787 |
| AccBSI | CCGCTC | 1 cut(s) | 3460 |
| AccI | GTMKAC | 1 cut(s) | 825 |
| AccII | CGCG | 1 cut(s) | 3963 |
| AcoI | YGGCCR | 3 cut(s) | 757, 898, 1912 |
| AcuI | CTGAAG | 9 cut(s) | 396, 1290, 1920, 2127, 2310, 2823, 2991, 3372, 4188 |
| AcyI | GRCGYC | 1 cut(s) | 34 |
| AfeI | AGCGCT | 1 cut(s) | 728 |
| AflII | CTTAAG | 1 cut(s) | 2245 |
| AflIII | ACRYGT | 2 cut(s) | 45, 3928 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3345 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1561, 1593 |
| Alw21I | GWGCWC | 4 cut(s) | 160, 707, 1093, 4520 |
| Alw44I | GTGCAC | 2 cut(s) | 156, 1089 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 305, 932, 3497, 3665 |
| Ama87I | CYCGRG | 2 cut(s) | 1926, 3484 |
| Aor51HI | AGCGCT | 1 cut(s) | 728 |
| ApaLI | GTGCAC | 2 cut(s) | 156, 1089 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 123, 129, 155, 161, 1454, 1486 |
| AseI | ATTAAT | 2 cut(s) | 495, 4110 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 996 |
| Asp718I | GGTACC | 1 cut(s) | 1787 |
| AspLEI | GCGC | 3 cut(s) | 729, 2777, 2845 |
| AspS9I | GGNCC | 8 cut(s) | 1772, 2195, 4142, 4403, 4421, 4604, 4708, 4727 |
| AsuC2I | CCSGG | 3 cut(s) | 4146, 4608, 4726 |
| AsuII | TTCGAA | 1 cut(s) | 4491 |
| AvaI | CYCGRG | 2 cut(s) | 1926, 3484 |
| AvaII | GGWCC | 4 cut(s) | 1772, 2195, 4403, 4604 |
| AxyI | CCTNAGG | 1 cut(s) | 174 |
| BaeGI | GKGCMC | 3 cut(s) | 160, 1093, 4570 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 3486, 3519 |
| BalI | TGGCCA | 1 cut(s) | 759 |
| BanI | GGYRCC | 1 cut(s) | 1787 |
| BanII | GRGCYC | 3 cut(s) | 707, 3780, 4520 |
| BauI | CACGAG | 1 cut(s) | 3372 |
| BbsI | GAAGAC | 3 cut(s) | 313, 1431, 1724 |
| Bbv12I | GWGCWC | 4 cut(s) | 160, 707, 1093, 4520 |
| BbvCI | CCTCAGC | 1 cut(s) | 18 |
| BceAI | ACGGC | 3 cut(s) | 47, 1257, 4435 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 546, 580 |
| BciVI | GTATCC | 2 cut(s) | 1486, 1846 |
| BclI | TGATCA | 2 cut(s) | 697, 1612 |
| BcnI | CCSGG | 3 cut(s) | 4146, 4608, 4726 |
| BfmI | CTRYAG | 6 cut(s) | 78, 1986, 2520, 3747, 4414, 4443 |
| BfoI | RGCGCY | 1 cut(s) | 730 |
| BfrI | CTTAAG | 1 cut(s) | 2245 |
| BfuAI | ACCTGC | 5 cut(s) | 274, 358, 3029, 3685, 3778 |
| BfuI | GTATCC | 2 cut(s) | 1486, 1846 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 3086 |
| BlpI | GCTNAGC | 1 cut(s) | 3774 |
| BmcAI | AGTACT | 1 cut(s) | 2053 |
| Bme18I | GGWCC | 4 cut(s) | 1772, 2195, 4403, 4604 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3345 |
| BmeT110I | CYCGRG | 2 cut(s) | 1926, 3484 |
| BmgT120I | GGNCC | 8 cut(s) | 1772, 2195, 4142, 4403, 4421, 4604, 4708, 4727 |
| BmiI | GGNNCC | 6 cut(s) | 1633, 1789, 4144, 4422, 4605, 4866 |
| BmrI | ACTGGG | 2 cut(s) | 2471, 4791 |
| BmuI | ACTGGG | 2 cut(s) | 2471, 4791 |
| BpiI | GAAGAC | 3 cut(s) | 313, 1431, 1724 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1343, 1375, 2395, 2427 |
| BpmI | CTGGAG | 8 cut(s) | 2116, 2334, 2376, 2625, 3603, 3990, 4102, 4752 |
| Bpu10I | CCTNAGC | 3 cut(s) | 18, 968, 1750 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 3774 |
| Bpu14I | TTCGAA | 1 cut(s) | 4491 |
| BpuEI | CTTGAG | 2 cut(s) | 1386, 1645 |
| BpuMI | CCSGG | 3 cut(s) | 4146, 4608, 4726 |
| BsaAI | YACGTR | 1 cut(s) | 1163 |
| BsaHI | GRCGYC | 1 cut(s) | 34 |
| BsaI | GGTCTC | 2 cut(s) | 298, 3957 |
| BsaWI | WCCGGW | 2 cut(s) | 712, 769 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 807, 837, 1561, 1591, 2307, 2337, 3709, 3739 |
| Bse118I | RCCGGY | 1 cut(s) | 1745 |
| Bse1I | ACTGG | 7 cut(s) | 1098, 1342, 1915, 2133, 2466, 4390, 4797 |
| Bse21I | CCTNAGG | 1 cut(s) | 174 |
| Bse3DI | GCAATG | 2 cut(s) | 1732, 2546 |
| BseMI | GCAATG | 2 cut(s) | 1732, 2546 |
| BseNI | ACTGG | 7 cut(s) | 1098, 1342, 1915, 2133, 2466, 4390, 4797 |
| BseRI | GAGGAG | 5 cut(s) | 35, 1079, 1389, 2622, 3470 |
| BseSI | GKGCMC | 3 cut(s) | 160, 1093, 4570 |
| BseYI | CCCAGC | 4 cut(s) | 2041, 2527, 4185, 4355 |
| BsgI | GTGCAG | 5 cut(s) | 454, 581, 1284, 3909, 4158 |
| Bsh1236I | CGCG | 1 cut(s) | 3963 |
| Bsh1285I | CGRYCG | 1 cut(s) | 4894 |
| BshNI | GGYRCC | 1 cut(s) | 1787 |
| BsiEI | CGRYCG | 1 cut(s) | 4894 |
| BsiHKAI | GWGCWC | 4 cut(s) | 160, 707, 1093, 4520 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1926, 3484 |
| BsiSI | CCGG | 7 cut(s) | 713, 770, 1746, 1830, 4146, 4607, 4725 |
| BslFI | GGGAC | 6 cut(s) | 41, 3373, 3680, 4177, 4292, 4617 |
| BsmFI | GGGAC | 6 cut(s) | 41, 3373, 3680, 4177, 4292, 4617 |
| BsmI | GAATGC | 7 cut(s) | 383, 598, 1518, 2263, 2854, 3484, 3761 |
| Bso31I | GGTCTC | 2 cut(s) | 298, 3957 |
| BsoBI | CYCGRG | 2 cut(s) | 1926, 3484 |
| Bsp119I | TTCGAA | 1 cut(s) | 4491 |
| Bsp1286I | GDGCHC | 6 cut(s) | 160, 707, 1093, 3780, 4520, 4570 |
| Bsp1407I | TGTACA | 1 cut(s) | 961 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 3774 |
| Bsp19I | CCATGG | 1 cut(s) | 3228 |
| BspFNI | CGCG | 1 cut(s) | 3963 |
| BspHI | TCATGA | 3 cut(s) | 1852, 2821, 3955 |
| BspLI | GGNNCC | 6 cut(s) | 1633, 1789, 4144, 4422, 4605, 4866 |
| BspMAI | CTGCAG | 3 cut(s) | 1990, 2524, 4447 |
| BspMI | ACCTGC | 5 cut(s) | 274, 358, 3029, 3685, 3778 |
| BspQI | GCTCTTC | 2 cut(s) | 1423, 1708 |
| BspT104I | TTCGAA | 1 cut(s) | 4491 |
| BspT107I | GGYRCC | 1 cut(s) | 1787 |
| BspTI | CTTAAG | 1 cut(s) | 2245 |
| BspTNI | GGTCTC | 2 cut(s) | 298, 3957 |
| BsrBI | CCGCTC | 1 cut(s) | 3460 |
| BsrDI | GCAATG | 2 cut(s) | 1732, 2546 |
| BsrFI | RCCGGY | 1 cut(s) | 1745 |
| BsrGI | TGTACA | 1 cut(s) | 961 |
| BsrI | ACTGG | 7 cut(s) | 1098, 1342, 1915, 2133, 2466, 4390, 4797 |
| BssAI | RCCGGY | 1 cut(s) | 1745 |
| BssNI | GRCGYC | 1 cut(s) | 34 |
| BssSI | CACGAG | 1 cut(s) | 3372 |
| BssT1I | CCWWGG | 6 cut(s) | 949, 2019, 2712, 3132, 3228, 4913 |
| Bst2BI | CACGAG | 1 cut(s) | 3372 |
| Bst6I | CTCTTC | 6 cut(s) | 100, 433, 1423, 1708, 3137, 4850 |
| BstACI | GRCGYC | 1 cut(s) | 34 |
| BstAFI | CTTAAG | 1 cut(s) | 2245 |
| BstAUI | TGTACA | 1 cut(s) | 961 |
| BstBAI | YACGTR | 1 cut(s) | 1163 |
| BstBI | TTCGAA | 1 cut(s) | 4491 |
| BstC8I | GCNNGC | 8 cut(s) | 15, 209, 757, 898, 1747, 2592, 2895, 4660 |
| BstDSI | CCRYGG | 2 cut(s) | 1273, 3228 |
| BstEII | GGTNACC | 1 cut(s) | 4388 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 192, 3681, 4814 |
| BstFNI | CGCG | 1 cut(s) | 3963 |
| BstH2I | RGCGCY | 1 cut(s) | 730 |
| BstHHI | GCGC | 3 cut(s) | 729, 2777, 2845 |
| BstMCI | CGRYCG | 1 cut(s) | 4894 |
| BstNSI | RCATGY | 2 cut(s) | 49, 3932 |
| BstPI | GGTNACC | 1 cut(s) | 4388 |
| BstSFI | CTRYAG | 6 cut(s) | 78, 1986, 2520, 3747, 4414, 4443 |
| BstSLI | GKGCMC | 3 cut(s) | 160, 1093, 4570 |
| BstSNI | TACGTA | 1 cut(s) | 1163 |
| BstUI | CGCG | 1 cut(s) | 3963 |
| BstV2I | GAAGAC | 3 cut(s) | 313, 1431, 1724 |
| BstX2I | RGATCY | 3 cut(s) | 1954, 3577, 3743 |
| BstYI | RGATCY | 3 cut(s) | 1954, 3577, 3743 |
| Bsu36I | CCTNAGG | 1 cut(s) | 174 |
| BsuI | GTATCC | 2 cut(s) | 1486, 1846 |
| BtgI | CCRYGG | 2 cut(s) | 1273, 3228 |
| BtgZI | GCGATG | 1 cut(s) | 342 |
| BtsI | GCAGTG | 3 cut(s) | 564, 4207, 4452 |
| BveI | ACCTGC | 5 cut(s) | 274, 358, 3029, 3685, 3778 |
| Cac8I | GCNNGC | 8 cut(s) | 15, 209, 757, 898, 1747, 2592, 2895, 4660 |
| CaiI | CAGNNNCTG | 4 cut(s) | 305, 932, 3497, 3665 |
| CciI | TCATGA | 3 cut(s) | 1852, 2821, 3955 |
| CfoI | GCGC | 3 cut(s) | 729, 2777, 2845 |
| Cfr10I | RCCGGY | 1 cut(s) | 1745 |
| Cfr13I | GGNCC | 8 cut(s) | 1772, 2195, 4142, 4403, 4421, 4604, 4708, 4727 |
| CseI | GACGC | 3 cut(s) | 23, 141, 3296 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1608, 1643 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3345 |
| EaeI | YGGCCR | 3 cut(s) | 757, 898, 1912 |
| Eam1104I | CTCTTC | 6 cut(s) | 100, 433, 1423, 1708, 3137, 4850 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3345 |
| EarI | CTCTTC | 6 cut(s) | 100, 433, 1423, 1708, 3137, 4850 |
| Ecl136II | GAGCTC | 2 cut(s) | 705, 4518 |
| Eco105I | TACGTA | 1 cut(s) | 1163 |
| Eco130I | CCWWGG | 6 cut(s) | 949, 2019, 2712, 3132, 3228, 4913 |
| Eco24I | GRGCYC | 3 cut(s) | 707, 3780, 4520 |
| Eco31I | GGTCTC | 2 cut(s) | 298, 3957 |
| Eco32I | GATATC | 2 cut(s) | 420, 4015 |
| Eco47I | GGWCC | 4 cut(s) | 1772, 2195, 4403, 4604 |
| Eco47III | AGCGCT | 1 cut(s) | 728 |
| Eco53kI | GAGCTC | 2 cut(s) | 705, 4518 |
| Eco57I | CTGAAG | 9 cut(s) | 396, 1290, 1920, 2127, 2310, 2823, 2991, 3372, 4188 |
| Eco81I | CCTNAGG | 1 cut(s) | 174 |
| Eco88I | CYCGRG | 2 cut(s) | 1926, 3484 |
| Eco91I | GGTNACC | 1 cut(s) | 4388 |
| EcoICRI | GAGCTC | 2 cut(s) | 705, 4518 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 192, 3681, 4814 |
| EcoO109I | RGGNCCY | 2 cut(s) | 4421, 4708 |
| EcoO65I | GGTNACC | 1 cut(s) | 4388 |
| EcoRI | GAATTC | 3 cut(s) | 2407, 3117, 3308 |
| EcoRV | GATATC | 2 cut(s) | 420, 4015 |
| EcoT14I | CCWWGG | 6 cut(s) | 949, 2019, 2712, 3132, 3228, 4913 |
| EcoT22I | ATGCAT | 2 cut(s) | 6, 552 |
| EcoT38I | GRGCYC | 3 cut(s) | 707, 3780, 4520 |
| ErhI | CCWWGG | 6 cut(s) | 949, 2019, 2712, 3132, 3228, 4913 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 804, 836, 2020, 2052, 2775, 2807, 3497, 3529 |
| FaqI | GGGAC | 6 cut(s) | 41, 3373, 3680, 4177, 4292, 4617 |
| FauI | CCCGC | 1 cut(s) | 2544 |
| FauNDI | CATATG | 2 cut(s) | 2233, 4543 |
| FbaI | TGATCA | 2 cut(s) | 697, 1612 |
| FblI | GTMKAC | 1 cut(s) | 825 |
| FriOI | GRGCYC | 3 cut(s) | 707, 3780, 4520 |
| GlaI | GCGC | 3 cut(s) | 728, 2776, 2844 |
| GsaI | CCCAGC | 4 cut(s) | 2045, 2531, 4189, 4359 |
| GsuI | CTGGAG | 8 cut(s) | 2116, 2334, 2376, 2625, 3603, 3990, 4102, 4752 |
| HaeII | RGCGCY | 1 cut(s) | 730 |
| HapII | CCGG | 7 cut(s) | 713, 770, 1746, 1830, 4146, 4607, 4725 |
| HgaI | GACGC | 3 cut(s) | 23, 141, 3296 |
| HhaI | GCGC | 3 cut(s) | 729, 2777, 2845 |
| Hin1I | GRCGYC | 1 cut(s) | 34 |
| Hin6I | GCGC | 3 cut(s) | 727, 2775, 2843 |
| HinP1I | GCGC | 3 cut(s) | 727, 2775, 2843 |
| HincII | GTYRAC | 5 cut(s) | 477, 1210, 1458, 1870, 2489 |
| HindII | GTYRAC | 5 cut(s) | 477, 1210, 1458, 1870, 2489 |
| HindIII | AAGCTT | 2 cut(s) | 278, 2729 |
| HpaI | GTTAAC | 1 cut(s) | 477 |
| HpaII | CCGG | 7 cut(s) | 713, 770, 1746, 1830, 4146, 4607, 4725 |
| Hpy99I | CGWCG | 2 cut(s) | 39, 42 |
| HpyCH4IV | ACGT | 2 cut(s) | 721, 1162 |
| HpySE526I | ACGT | 2 cut(s) | 721, 1162 |
| Hsp92I | GRCGYC | 1 cut(s) | 34 |
| HspAI | GCGC | 3 cut(s) | 727, 2775, 2843 |
| KpnI | GGTACC | 1 cut(s) | 1791 |
| KroI | GCCGGC | 1 cut(s) | 1745 |
| KroNI | GCCGGC | 1 cut(s) | 1747 |
| Ksp22I | TGATCA | 2 cut(s) | 697, 1612 |
| KspAI | GTTAAC | 1 cut(s) | 477 |
| LguI | GCTCTTC | 2 cut(s) | 1423, 1708 |
| MaeII | ACGT | 2 cut(s) | 721, 1162 |
| MbiI | CCGCTC | 1 cut(s) | 3460 |
| MflI | RGATCY | 3 cut(s) | 1954, 3577, 3743 |
| MhlI | GDGCHC | 6 cut(s) | 160, 707, 1093, 3780, 4520, 4570 |
| MlsI | TGGCCA | 1 cut(s) | 759 |
| MluNI | TGGCCA | 1 cut(s) | 759 |
| MlyI | GAGTC | 7 cut(s) | 32, 2537, 2882, 3137, 4015, 4109, 4377 |
| MmeI | TCCRAC | 6 cut(s) | 90, 111, 127, 877, 3408, 3790 |
| Mox20I | TGGCCA | 1 cut(s) | 759 |
| Mph1103I | ATGCAT | 2 cut(s) | 6, 552 |
| MroNI | GCCGGC | 1 cut(s) | 1745 |
| MroXI | GAANNNNTTC | 1 cut(s) | 996 |
| MscI | TGGCCA | 1 cut(s) | 759 |
| MslI | CAYNNNNRTG | 3 cut(s) | 339, 430, 2389 |
| Msp20I | TGGCCA | 1 cut(s) | 759 |
| MspA1I | CMGCKG | 2 cut(s) | 17, 2630 |
| MspCI | CTTAAG | 1 cut(s) | 2245 |
| MspI | CCGG | 7 cut(s) | 713, 770, 1746, 1830, 4146, 4607, 4725 |
| Mva1269I | GAATGC | 7 cut(s) | 383, 598, 1518, 2263, 2854, 3484, 3761 |
| MvnI | CGCG | 1 cut(s) | 3963 |
| NaeI | GCCGGC | 1 cut(s) | 1747 |
| NciI | CCSGG | 3 cut(s) | 4146, 4608, 4726 |
| NcoI | CCATGG | 1 cut(s) | 3228 |
| NdeI | CATATG | 2 cut(s) | 2233, 4543 |
| NgoMIV | GCCGGC | 1 cut(s) | 1745 |
| NlaIV | GGNNCC | 6 cut(s) | 1633, 1789, 4144, 4422, 4605, 4866 |
| NmuCI | GTSAC | 6 cut(s) | 2078, 2540, 3233, 3347, 3381, 4359 |
| NsiI | ATGCAT | 2 cut(s) | 6, 552 |
| NspI | RCATGY | 2 cut(s) | 49, 3932 |
| NspV | TTCGAA | 1 cut(s) | 4491 |
| PacI | TTAATTAA | 1 cut(s) | 735 |
| PagI | TCATGA | 3 cut(s) | 1852, 2821, 3955 |
| PaqCI | CACCTGC | 1 cut(s) | 3685 |
| PciI | ACATGT | 2 cut(s) | 45, 3928 |
| PciSI | GCTCTTC | 2 cut(s) | 1423, 1708 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 139 |
| PctI | GAATGC | 7 cut(s) | 383, 598, 1518, 2263, 2854, 3484, 3761 |
| PdiI | GCCGGC | 1 cut(s) | 1747 |
| PdmI | GAANNNNTTC | 1 cut(s) | 996 |
| PfeI | GAWTC | 8 cut(s) | 179, 1111, 1253, 2641, 3068, 3440, 4054, 4550 |
| PfoI | TCCNGGA | 3 cut(s) | 2197, 3580, 4279 |
| Ple19I | CGATCG | 1 cut(s) | 4894 |
| PleI | GAGTC | 7 cut(s) | 31, 2537, 2881, 3136, 4014, 4109, 4376 |
| PpsI | GAGTC | 7 cut(s) | 31, 2537, 2881, 3136, 4014, 4109, 4376 |
| Ppu21I | YACGTR | 1 cut(s) | 1163 |
| PscI | ACATGT | 2 cut(s) | 45, 3928 |
| PshBI | ATTAAT | 2 cut(s) | 495, 4110 |
| Psp124BI | GAGCTC | 2 cut(s) | 707, 4520 |
| PspEI | GGTNACC | 1 cut(s) | 4388 |
| PspFI | CCCAGC | 4 cut(s) | 2041, 2527, 4185, 4355 |
| PspN4I | GGNNCC | 6 cut(s) | 1633, 1789, 4144, 4422, 4605, 4866 |
| PspPI | GGNCC | 8 cut(s) | 1772, 2195, 4142, 4403, 4421, 4604, 4708, 4727 |
| PstI | CTGCAG | 3 cut(s) | 1990, 2524, 4447 |
| PstNI | CAGNNNCTG | 4 cut(s) | 305, 932, 3497, 3665 |
| PsuI | RGATCY | 3 cut(s) | 1954, 3577, 3743 |
| PvuI | CGATCG | 1 cut(s) | 4894 |
| PvuII | CAGCTG | 2 cut(s) | 17, 2630 |
| RseI | CAYNNNNRTG | 3 cut(s) | 339, 430, 2389 |
| SacI | GAGCTC | 2 cut(s) | 707, 4520 |
| SapI | GCTCTTC | 2 cut(s) | 1423, 1708 |
| Sau96I | GGNCC | 8 cut(s) | 1772, 2195, 4142, 4403, 4421, 4604, 4708, 4727 |
| ScaI | AGTACT | 1 cut(s) | 2053 |
| SchI | GAGTC | 7 cut(s) | 32, 2537, 2882, 3137, 4015, 4109, 4377 |
| SduI | GDGCHC | 6 cut(s) | 160, 707, 1093, 3780, 4520, 4570 |
| SfcI | CTRYAG | 6 cut(s) | 78, 1986, 2520, 3747, 4414, 4443 |
| SfuI | TTCGAA | 1 cut(s) | 4491 |
| SinI | GGWCC | 4 cut(s) | 1772, 2195, 4403, 4604 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 339, 430, 2389 |
| SmlI | CTYRAG | 3 cut(s) | 1401, 1660, 2245 |
| SmoI | CTYRAG | 3 cut(s) | 1401, 1660, 2245 |
| SnaBI | TACGTA | 1 cut(s) | 1163 |
| SspI | AATATT | 2 cut(s) | 906, 3076 |
| SstI | GAGCTC | 2 cut(s) | 707, 4520 |
| StyI | CCWWGG | 6 cut(s) | 949, 2019, 2712, 3132, 3228, 4913 |
| TaiI | ACGT | 2 cut(s) | 724, 1165 |
| TaqI | TCGA | 7 cut(s) | 26, 262, 1708, 3984, 4491, 4514, 4890 |
| TatI | WGTACW | 3 cut(s) | 961, 2051, 4426 |
| TauI | GCSGC | 6 cut(s) | 1701, 1917, 2354, 2893, 3463, 3855 |
| TfiI | GAWTC | 8 cut(s) | 179, 1111, 1253, 2641, 3068, 3440, 4054, 4550 |
| TseFI | GTSAC | 6 cut(s) | 2078, 2540, 3233, 3347, 3381, 4359 |
| Tsp45I | GTSAC | 6 cut(s) | 2078, 2540, 3233, 3347, 3381, 4359 |
| TspGWI | ACGGA | 7 cut(s) | 360, 588, 1375, 1582, 2566, 3290, 4765 |
| Vha464I | CTTAAG | 1 cut(s) | 2245 |
| VneI | GTGCAC | 2 cut(s) | 156, 1089 |
| VpaK11BI | GGWCC | 4 cut(s) | 1772, 2195, 4403, 4604 |
| VspI | ATTAAT | 2 cut(s) | 495, 4110 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 192, 3681, 4814 |
| XceI | RCATGY | 2 cut(s) | 49, 3932 |
| XmiI | GTMKAC | 1 cut(s) | 825 |
| XmnI | GAANNNNTTC | 1 cut(s) | 996 |
| ZrmI | AGTACT | 1 cut(s) | 2053 |
| Zsp2I | ATGCAT | 2 cut(s) | 6, 552 |