Trypsin
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1439 |
| AatII | GACGTC | 1 cut(s) | 230 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 713 |
| AccII | CGCG | 1 cut(s) | 160 |
| AciI | CCGC | 7 cut(s) | 56, 69, 330, 738, 1036, 1075, 1098 |
| AclWI | GGATC | 1 cut(s) | 787 |
| AcoI | YGGCCR | 1 cut(s) | 579 |
| AcsI | RAATTY | 3 cut(s) | 7, 829, 1427 |
| AcuI | CTGAAG | 1 cut(s) | 159 |
| AcyI | GRCGYC | 1 cut(s) | 227 |
| AfaI | GTAC | 3 cut(s) | 945, 1232, 1352 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 713, 998 |
| AgeI | ACCGGT | 1 cut(s) | 934 |
| AgsI | TTSAA | 8 cut(s) | 196, 309, 372, 892, 956, 1120, 1288, 1600 |
| AjnI | CCWGG | 1 cut(s) | 1560 |
| AleI | CACNNNNGTG | 2 cut(s) | 935, 1332 |
| Alw26I | GTCTC | 1 cut(s) | 1390 |
| AlwI | GGATC | 1 cut(s) | 787 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 137, 212 |
| AoxI | GGCC | 1 cut(s) | 579 |
| ApeKI | GCWGC | 3 cut(s) | 332, 812, 1492 |
| ApoI | RAATTY | 3 cut(s) | 7, 829, 1427 |
| AseI | ATTAAT | 1 cut(s) | 819 |
| AsiGI | ACCGGT | 1 cut(s) | 934 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 324 |
| AspS9I | GGNCC | 1 cut(s) | 839 |
| AsuC2I | CCSGG | 1 cut(s) | 751 |
| AsuHPI | GGTGA | 5 cut(s) | 225, 293, 674, 728, 949 |
| AvaII | GGWCC | 1 cut(s) | 839 |
| BalI | TGGCCA | 1 cut(s) | 581 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 90, 122 |
| BauI | CACGAG | 1 cut(s) | 1250 |
| BbsI | GAAGAC | 1 cut(s) | 248 |
| BbvI | GCAGC | 3 cut(s) | 319, 824, 1504 |
| BccI | CCATC | 6 cut(s) | 157, 748, 1012, 1142, 1163, 1609 |
| BceAI | ACGGC | 1 cut(s) | 1108 |
| BciT130I | CCWGG | 1 cut(s) | 1562 |
| BclI | TGATCA | 1 cut(s) | 1663 |
| BcnI | CCSGG | 1 cut(s) | 751 |
| BcoDI | GTCTC | 1 cut(s) | 1390 |
| BfmI | CTRYAG | 1 cut(s) | 570 |
| BglII | AGATCT | 1 cut(s) | 248 |
| BisI | GCNGC | 4 cut(s) | 70, 333, 813, 1493 |
| BlsI | GCNGC | 4 cut(s) | 71, 334, 814, 1494 |
| Bme1390I | CCNGG | 2 cut(s) | 751, 1562 |
| Bme18I | GGWCC | 1 cut(s) | 839 |
| BmgT120I | GGNCC | 1 cut(s) | 839 |
| BmrFI | CCNGG | 2 cut(s) | 751, 1562 |
| BmsI | GCATC | 7 cut(s) | 34, 110, 367, 526, 673, 799, 892 |
| BpiI | GAAGAC | 1 cut(s) | 248 |
| BpmI | CTGGAG | 1 cut(s) | 1257 |
| Bpu10I | CCTNAGC | 1 cut(s) | 120 |
| BpuEI | CTTGAG | 3 cut(s) | 510, 521, 1742 |
| BpuMI | CCSGG | 1 cut(s) | 751 |
| Bsa29I | ATCGAT | 1 cut(s) | 323 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1662 |
| BsaHI | GRCGYC | 1 cut(s) | 227 |
| BsaJI | CCNNGG | 3 cut(s) | 428, 1482, 1560 |
| BsaWI | WCCGGW | 1 cut(s) | 934 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 749, 779 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 713, 998 |
| Bse118I | RCCGGY | 2 cut(s) | 65, 934 |
| Bse1I | ACTGG | 1 cut(s) | 1240 |
| Bse3DI | GCAATG | 4 cut(s) | 298, 600, 1476, 1726 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1662 |
| BseBI | CCWGG | 1 cut(s) | 1562 |
| BseCI | ATCGAT | 1 cut(s) | 323 |
| BseDI | CCNNGG | 3 cut(s) | 428, 1482, 1560 |
| BseGI | GGATG | 6 cut(s) | 303, 382, 628, 759, 1620, 1639 |
| BseJI | GATNNNNATC | 1 cut(s) | 1662 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 713, 998 |
| BseMI | GCAATG | 4 cut(s) | 298, 600, 1476, 1726 |
| BseMII | CTCAG | 4 cut(s) | 111, 399, 897, 1398 |
| BseNI | ACTGG | 1 cut(s) | 1240 |
| BseXI | GCAGC | 3 cut(s) | 319, 824, 1504 |
| BseYI | CCCAGC | 1 cut(s) | 1610 |
| Bsh1236I | CGCG | 1 cut(s) | 160 |
| BshFI | GGCC | 1 cut(s) | 581 |
| BshTI | ACCGGT | 1 cut(s) | 934 |
| BshVI | ATCGAT | 1 cut(s) | 323 |
| BsiSI | CCGG | 3 cut(s) | 66, 751, 935 |
| BslFI | GGGAC | 1 cut(s) | 1186 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 713, 998 |
| BsmAI | GTCTC | 1 cut(s) | 1390 |
| BsmFI | GGGAC | 1 cut(s) | 1186 |
| BsnI | GGCC | 1 cut(s) | 581 |
| Bsp1407I | TGTACA | 1 cut(s) | 943 |
| Bsp143I | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| Bsp19I | CCATGG | 1 cut(s) | 428 |
| Bsp68I | TCGCGA | 1 cut(s) | 160 |
| BspACI | CCGC | 7 cut(s) | 56, 69, 330, 738, 1036, 1075, 1098 |
| BspANI | GGCC | 1 cut(s) | 581 |
| BspCNI | CTCAG | 5 cut(s) | 4, 112, 400, 898, 1399 |
| BspDI | ATCGAT | 1 cut(s) | 323 |
| BspFNI | CGCG | 1 cut(s) | 160 |
| BspPI | GGATC | 1 cut(s) | 787 |
| BsrDI | GCAATG | 4 cut(s) | 298, 600, 1476, 1726 |
| BsrFI | RCCGGY | 2 cut(s) | 65, 934 |
| BsrGI | TGTACA | 1 cut(s) | 943 |
| BsrI | ACTGG | 1 cut(s) | 1240 |
| BssAI | RCCGGY | 2 cut(s) | 65, 934 |
| BssECI | CCNNGG | 3 cut(s) | 428, 1482, 1560 |
| BssMI | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| BssNI | GRCGYC | 1 cut(s) | 227 |
| BssSI | CACGAG | 1 cut(s) | 1250 |
| BssT1I | CCWWGG | 1 cut(s) | 428 |
| Bst2BI | CACGAG | 1 cut(s) | 1250 |
| Bst2UI | CCWGG | 1 cut(s) | 1562 |
| Bst4CI | ACNGT | 7 cut(s) | 352, 447, 574, 616, 694, 1177, 1384 |
| Bst6I | CTCTTC | 3 cut(s) | 11, 481, 1539 |
| BstACI | GRCGYC | 1 cut(s) | 227 |
| BstAUI | TGTACA | 1 cut(s) | 943 |
| BstC8I | GCNNGC | 2 cut(s) | 67, 1366 |
| BstDEI | CTNAG | 8 cut(s) | 12, 120, 408, 558, 906, 1139, 1407, 1686 |
| BstDSI | CCRYGG | 2 cut(s) | 428, 1482 |
| BstEII | GGTNACC | 1 cut(s) | 215 |
| BstF5I | GGATG | 6 cut(s) | 303, 382, 628, 759, 1620, 1639 |
| BstFNI | CGCG | 1 cut(s) | 160 |
| BstKTI | GATC | 5 cut(s) | 251, 782, 1406, 1666, 1756 |
| BstMAI | GTCTC | 1 cut(s) | 1390 |
| BstMBI | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 53, 62, 98, 140, 1700 |
| BstNI | CCWGG | 1 cut(s) | 1562 |
| BstPI | GGTNACC | 1 cut(s) | 215 |
| BstSCI | CCNGG | 2 cut(s) | 749, 1560 |
| BstSFI | CTRYAG | 1 cut(s) | 570 |
| BstUI | CGCG | 1 cut(s) | 160 |
| BstV1I | GCAGC | 3 cut(s) | 319, 824, 1504 |
| BstV2I | GAAGAC | 1 cut(s) | 248 |
| BstX2I | RGATCY | 1 cut(s) | 248 |
| BstYI | RGATCY | 1 cut(s) | 248 |
| Bsu15I | ATCGAT | 1 cut(s) | 323 |
| BsuRI | GGCC | 1 cut(s) | 581 |
| BsuTUI | ATCGAT | 1 cut(s) | 323 |
| BtgI | CCRYGG | 2 cut(s) | 428, 1482 |
| BtsCI | GGATG | 6 cut(s) | 303, 382, 628, 759, 1620, 1639 |
| BtsIMutI | CAGTG | 2 cut(s) | 405, 1233 |
| BtuMI | TCGCGA | 1 cut(s) | 160 |
| Cac8I | GCNNGC | 2 cut(s) | 67, 1366 |
| CaiI | CAGNNNCTG | 2 cut(s) | 137, 212 |
| Cfr10I | RCCGGY | 2 cut(s) | 65, 934 |
| Cfr13I | GGNCC | 1 cut(s) | 839 |
| ClaI | ATCGAT | 1 cut(s) | 323 |
| CseI | GACGC | 2 cut(s) | 171, 1442 |
| Csp6I | GTAC | 3 cut(s) | 944, 1231, 1351 |
| CspAI | ACCGGT | 1 cut(s) | 934 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 526, 561 |
| CviAII | CATG | 5 cut(s) | 429, 776, 971, 1190, 1466 |
| CviQI | GTAC | 3 cut(s) | 944, 1231, 1351 |
| DdeI | CTNAG | 8 cut(s) | 12, 120, 408, 558, 906, 1139, 1407, 1686 |
| DpnI | GATC | 5 cut(s) | 250, 781, 1405, 1665, 1755 |
| DpnII | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1439 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1439 |
| EaeI | YGGCCR | 1 cut(s) | 579 |
| Eam1104I | CTCTTC | 3 cut(s) | 11, 481, 1539 |
| EarI | CTCTTC | 3 cut(s) | 11, 481, 1539 |
| EciI | GGCGGA | 1 cut(s) | 71 |
| Eco130I | CCWWGG | 1 cut(s) | 428 |
| Eco47I | GGWCC | 1 cut(s) | 839 |
| Eco57I | CTGAAG | 1 cut(s) | 159 |
| Eco91I | GGTNACC | 1 cut(s) | 215 |
| EcoO65I | GGTNACC | 1 cut(s) | 215 |
| EcoRI | GAATTC | 2 cut(s) | 7, 829 |
| EcoRII | CCWGG | 1 cut(s) | 1560 |
| EcoT14I | CCWWGG | 1 cut(s) | 428 |
| ErhI | CCWWGG | 1 cut(s) | 428 |
| FaeI | CATG | 5 cut(s) | 432, 779, 974, 1193, 1469 |
| FaqI | GGGAC | 1 cut(s) | 1186 |
| FatI | CATG | 5 cut(s) | 428, 775, 970, 1189, 1465 |
| FauI | CCCGC | 1 cut(s) | 337 |
| FbaI | TGATCA | 1 cut(s) | 1663 |
| Fnu4HI | GCNGC | 4 cut(s) | 70, 333, 813, 1493 |
| FokI | GGATG | 6 cut(s) | 310, 389, 635, 766, 1627, 1646 |
| Fsp4HI | GCNGC | 4 cut(s) | 70, 333, 813, 1493 |
| GluI | GCNGC | 4 cut(s) | 70, 333, 813, 1493 |
| GsaI | CCCAGC | 1 cut(s) | 1614 |
| GsuI | CTGGAG | 1 cut(s) | 1257 |
| HaeIII | GGCC | 1 cut(s) | 581 |
| HapII | CCGG | 3 cut(s) | 66, 751, 935 |
| HgaI | GACGC | 2 cut(s) | 171, 1442 |
| Hin1I | GRCGYC | 1 cut(s) | 227 |
| Hin1II | CATG | 5 cut(s) | 432, 779, 974, 1193, 1469 |
| HincII | GTYRAC | 2 cut(s) | 612, 1555 |
| HindII | GTYRAC | 2 cut(s) | 612, 1555 |
| HindIII | AAGCTT | 1 cut(s) | 84 |
| HinfI | GANTC | 6 cut(s) | 203, 320, 325, 1055, 1106, 1241 |
| HpaII | CCGG | 3 cut(s) | 66, 751, 935 |
| HphI | GGTGA | 5 cut(s) | 225, 293, 674, 728, 949 |
| Hpy166II | GTNNAC | 6 cut(s) | 29, 403, 612, 1233, 1486, 1555 |
| Hpy188III | TCNNGA | 8 cut(s) | 159, 538, 956, 1028, 1252, 1655, 1679, 1759 |
| Hpy8I | GTNNAC | 6 cut(s) | 29, 403, 612, 1233, 1486, 1555 |
| Hpy99I | CGWCG | 3 cut(s) | 160, 165, 232 |
| HpyAV | CCTTC | 5 cut(s) | 378, 632, 1249, 1356, 1746 |
| HpyCH4III | ACNGT | 7 cut(s) | 352, 447, 574, 616, 694, 1177, 1384 |
| HpyCH4IV | ACGT | 2 cut(s) | 25, 227 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 53, 62, 98, 140, 1700 |
| HpyF3I | CTNAG | 8 cut(s) | 12, 120, 408, 558, 906, 1139, 1407, 1686 |
| HpySE526I | ACGT | 2 cut(s) | 25, 227 |
| Hsp92I | GRCGYC | 1 cut(s) | 227 |
| Hsp92II | CATG | 5 cut(s) | 432, 779, 974, 1193, 1469 |
| KroI | GCCGGC | 1 cut(s) | 65 |
| KroNI | GCCGGC | 1 cut(s) | 67 |
| Ksp22I | TGATCA | 1 cut(s) | 1663 |
| Kzo9I | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| LmnI | GCTCC | 1 cut(s) | 340 |
| Lsp1109I | GCAGC | 3 cut(s) | 319, 824, 1504 |
| LweI | GCATC | 7 cut(s) | 34, 110, 367, 526, 673, 799, 892 |
| MaeII | ACGT | 2 cut(s) | 25, 227 |
| MaeIII | GTNAC | 7 cut(s) | 105, 215, 231, 596, 616, 688, 730 |
| MalI | GATC | 5 cut(s) | 250, 781, 1405, 1665, 1755 |
| MboI | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| MfeI | CAATTG | 1 cut(s) | 708 |
| MflI | RGATCY | 1 cut(s) | 248 |
| MlsI | TGGCCA | 1 cut(s) | 581 |
| MluNI | TGGCCA | 1 cut(s) | 581 |
| MnlI | CCTC | 7 cut(s) | 14, 120, 170, 216, 1305, 1353, 1624 |
| Mox20I | TGGCCA | 1 cut(s) | 581 |
| MreI | CGCCGGCG | 1 cut(s) | 65 |
| MroNI | GCCGGC | 1 cut(s) | 65 |
| MroXI | GAANNNNTTC | 1 cut(s) | 324 |
| MscI | TGGCCA | 1 cut(s) | 581 |
| MslI | CAYNNNNRTG | 4 cut(s) | 900, 935, 1332, 1464 |
| Msp20I | TGGCCA | 1 cut(s) | 581 |
| MspA1I | CMGCKG | 1 cut(s) | 332 |
| MspI | CCGG | 3 cut(s) | 66, 751, 935 |
| MspR9I | CCNGG | 2 cut(s) | 751, 1562 |
| MunI | CAATTG | 1 cut(s) | 708 |
| MvaI | CCWGG | 1 cut(s) | 1562 |
| MvnI | CGCG | 1 cut(s) | 160 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 53, 62, 98, 140, 1700 |
| NaeI | GCCGGC | 1 cut(s) | 67 |
| NciI | CCSGG | 1 cut(s) | 751 |
| NcoI | CCATGG | 1 cut(s) | 428 |
| NdeII | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| NgoMIV | GCCGGC | 1 cut(s) | 65 |
| NlaIII | CATG | 5 cut(s) | 432, 779, 974, 1193, 1469 |
| NmuCI | GTSAC | 3 cut(s) | 231, 596, 730 |
| NruI | TCGCGA | 1 cut(s) | 160 |
| OliI | CACNNNNGTG | 2 cut(s) | 935, 1332 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PdiI | GCCGGC | 1 cut(s) | 67 |
| PdmI | GAANNNNTTC | 1 cut(s) | 324 |
| PfeI | GAWTC | 6 cut(s) | 203, 320, 325, 1055, 1106, 1241 |
| PflFI | GACNNNGTC | 2 cut(s) | 165, 229 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 713 |
| PfoI | TCCNGGA | 1 cut(s) | 749 |
| PinAI | ACCGGT | 1 cut(s) | 934 |
| PkrI | GCNGC | 4 cut(s) | 71, 334, 814, 1494 |
| PshBI | ATTAAT | 1 cut(s) | 819 |
| Psp6I | CCWGG | 1 cut(s) | 1560 |
| PspEI | GGTNACC | 1 cut(s) | 215 |
| PspFI | CCCAGC | 1 cut(s) | 1610 |
| PspGI | CCWGG | 1 cut(s) | 1560 |
| PspPI | GGNCC | 1 cut(s) | 839 |
| PstNI | CAGNNNCTG | 2 cut(s) | 137, 212 |
| PsuI | RGATCY | 1 cut(s) | 248 |
| PsyI | GACNNNGTC | 2 cut(s) | 165, 229 |
| RruI | TCGCGA | 1 cut(s) | 160 |
| RsaI | GTAC | 3 cut(s) | 945, 1232, 1352 |
| RsaNI | GTAC | 3 cut(s) | 944, 1231, 1351 |
| RseI | CAYNNNNRTG | 4 cut(s) | 900, 935, 1332, 1464 |
| SatI | GCNGC | 4 cut(s) | 70, 333, 813, 1493 |
| Sau3AI | GATC | 5 cut(s) | 248, 779, 1403, 1663, 1753 |
| Sau96I | GGNCC | 1 cut(s) | 839 |
| ScrFI | CCNGG | 2 cut(s) | 751, 1562 |
| SfaNI | GCATC | 7 cut(s) | 34, 110, 367, 526, 673, 799, 892 |
| SfcI | CTRYAG | 1 cut(s) | 570 |
| SgrAI | CRCCGGYG | 2 cut(s) | 65, 934 |
| SinI | GGWCC | 1 cut(s) | 839 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 900, 935, 1332, 1464 |
| SmlI | CTYRAG | 3 cut(s) | 525, 536, 1757 |
| SmoI | CTYRAG | 3 cut(s) | 525, 536, 1757 |
| SsiI | CCGC | 7 cut(s) | 56, 69, 330, 738, 1036, 1075, 1098 |
| SspI | AATATT | 2 cut(s) | 913, 1278 |
| StyD4I | CCNGG | 2 cut(s) | 749, 1560 |
| StyI | CCWWGG | 1 cut(s) | 428 |
| TaaI | ACNGT | 7 cut(s) | 352, 447, 574, 616, 694, 1177, 1384 |
| TaiI | ACGT | 2 cut(s) | 28, 230 |
| TaqI | TCGA | 2 cut(s) | 323, 1402 |
| TatI | WGTACW | 2 cut(s) | 943, 1230 |
| TauI | GCSGC | 1 cut(s) | 72 |
| TfiI | GAWTC | 6 cut(s) | 203, 320, 325, 1055, 1106, 1241 |
| TscAI | CASTG | 2 cut(s) | 412, 1240 |
| TseFI | GTSAC | 3 cut(s) | 231, 596, 730 |
| TseI | GCWGC | 3 cut(s) | 332, 812, 1492 |
| Tsp45I | GTSAC | 3 cut(s) | 231, 596, 730 |
| TspGWI | ACGGA | 3 cut(s) | 144, 635, 1471 |
| TspRI | CASTG | 2 cut(s) | 412, 1240 |
| Tth111I | GACNNNGTC | 2 cut(s) | 165, 229 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 713 |
| VpaK11BI | GGWCC | 1 cut(s) | 839 |
| VspI | ATTAAT | 1 cut(s) | 819 |
| XapI | RAATTY | 3 cut(s) | 7, 829, 1427 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1232 |
| XmnI | GAANNNNTTC | 1 cut(s) | 324 |
| ZraI | GACGTC | 1 cut(s) | 228 |