Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Reverse (-)

31827918 .. 31830796

Gene Structure 2879 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1178100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGGATGGAAATGGAGGGATTGCGACCATGCTTACTGTTGACGACAACGAGTTTTGGGAAGGAGTGTCACCAGTGGAGTTTGGGGATTTGCCTGCGCTTCAAGATGCCGTTACAGTTGTGGGTTACCCAATTGGGGGAGACACAATTTCAGTGACGAGTGGTGTTGTGTCACGAATAGAGATTCTGTCCTATGTTCATGGGTCTACTGAACTTCTGGGTTTGCAGATAGATGCGGCCATAAACTCTGGAAACTCAGGTGGGCCTGCTTTTAATGACAAGGGAAATTGCGTCGGTATCGCATTTCAGTCCCTTAAACATGAAGATGCGGAGAATATTGGTTATGTCATACCAACACCAGTCATTATGCACTTCATCCAAGATTATGAGAAGAATGGAGCATATACAGGATTTCCAATTCTCGGAGTTGAGTGGCAGAAGATGGAAAATCCTGATTTGCGTATTGCAATGGGCATGAAACCTGATCAGAAGGGTGTTCGTATAAGAAGAGTTGATCCCACTGCTCCGGAATCTCAGGTTCTGAAGCCATCTGATATCATTCTCAGCTTTGATGGAGTCAAGATTGCCAATGATGGAACAGTTCCTTTCCGGCATGGAGAACGCATAGGTTTTAGTTACCTTGTCTCTCAGAAATATACTGGAGATAATTCAGCGGTTAAAGTTCTGCGCAATACTGAGATTCTCAATTTTGACATCAAACTTGCATCCCACAAAAGACTAATCCCAGCACACAACAAGGGCAGACCTCCTTCATATTATATAATTGCAGGATTTGTTTTTACGGCTGTGTCTGTTCCCTATCTTCGATCTGAGTATGGAAAGGAATATGAATTTGAAGCTCCTGTCAAGCTTTTGGACAAAATGCTGCATTCATTGCCACAATCACCGGATGAGCAGCTCGTCGTTGTTTCGCAGGTGCTTGTGGCTGACATCAACATCGGTTATGAAGAAATAGTTAACACCCAGGTTCTTACTTTAAACGGTAAGCCTGTGAAGAACCTGAAGAACTTGGCAAGCATGGTAGAGAGCTGCGAGGACGAATTTTTGAAATTTGATTTAGAATACCAACAGGTGGTTGTCCTCCGAACAAAAACTGCTAAAGCAGCTACTCTGGATATTCTTGCTACACATTGCATACCCTCAGCAATGTCCGATGATCTGAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

396

Amino Acids

43.27

Weight (kDa)

5.01

Isoelectric Point (pI)

41.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	12 - 124	5.4e-09	Trypsin
Trypsin_2	PF13365	21 - 99	7.3e-12	Trypsin-like peptidase domain
PDZ_2	PF13180	149 - 240	1.1e-06	PDZ domain
PDZ_3	PF17815	247 - 392	9.1e-50	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	922
Acc16I	TGCGCA	1 cut(s)	686
Acc36I	ACCTGC	1 cut(s)	922
AccB7I	CCANNNNNTGG	1 cut(s)	1090
AccI	GTMKAC	1 cut(s)	203
AccIII	TCCGGA	1 cut(s)	523
AciI	CCGC	3 cut(s)	233, 326, 671
AclWI	GGATC	1 cut(s)	506
AcoI	YGGCCR	1 cut(s)	234
AcsI	RAATTY	3 cut(s)	848, 1058, 1067
AcuI	CTGAAG	2 cut(s)	560, 1040
AfiI	CCNNNNNNNGG	4 cut(s)	133, 134, 419, 1090
AgsI	TTSAA	3 cut(s)	101, 854, 1066
AjnI	CCWGG	1 cut(s)	981
AluBI	AGCT	6 cut(s)	564, 857, 868, 916, 1047, 1124
AluI	AGCT	6 cut(s)	564, 857, 868, 916, 1047, 1124
Alw26I	GTCTC	2 cut(s)	132, 646
AlwI	GGATC	1 cut(s)	506
AlwNI	CAGNNNCTG	1 cut(s)	538
Aor13HI	TCCGGA	1 cut(s)	523
AoxI	GGCC	2 cut(s)	234, 260
ApeKI	GCWGC	4 cut(s)	883, 913, 1047, 1121
ApoI	RAATTY	3 cut(s)	848, 1058, 1067
AspLEI	GCGC	2 cut(s)	97, 687
AspS9I	GGNCC	1 cut(s)	260
AsuHPI	GGTGA	2 cut(s)	60, 894
BbvCI	CCTCAGC	1 cut(s)	1159
BbvI	GCAGC	4 cut(s)	870, 925, 1034, 1133
BccI	CCATC	4 cut(s)	433, 553, 563, 584
BceAI	ACGGC	2 cut(s)	92, 816
BciT130I	CCWGG	1 cut(s)	983
BclI	TGATCA	1 cut(s)	481
BcoDI	GTCTC	2 cut(s)	132, 646
BfuAI	ACCTGC	1 cut(s)	922
BisI	GCNGC	5 cut(s)	234, 884, 914, 1048, 1122
BlsI	GCNGC	5 cut(s)	235, 885, 915, 1049, 1123
Bme1390I	CCNGG	1 cut(s)	983
BmgT120I	GGNCC	1 cut(s)	260
BmrFI	CCNGG	1 cut(s)	983
BmsI	GCATC	4 cut(s)	94, 220, 313, 731
BpmI	CTGGAG	1 cut(s)	678
Bpu10I	CCTNAGC	1 cut(s)	1159
BsaJI	CCNNGG	1 cut(s)	981
BsaWI	WCCGGW	2 cut(s)	523, 904
Bsc4I	CCNNNNNNNGG	4 cut(s)	133, 134, 419, 1090
Bse1I	ACTGG	3 cut(s)	71, 356, 661
Bse3DI	GCAATG	4 cut(s)	471, 890, 1147, 1170
BseAI	TCCGGA	1 cut(s)	523
BseBI	CCWGG	1 cut(s)	983
BseDI	CCNNGG	1 cut(s)	981
BseGI	GGATG	4 cut(s)	10, 372, 722, 913
BseLI	CCNNNNNNNGG	4 cut(s)	133, 134, 419, 1090
BseMI	GCAATG	4 cut(s)	471, 890, 1147, 1170
BseMII	CTCAG	7 cut(s)	267, 545, 574, 659, 684, 819, 1173
BseNI	ACTGG	3 cut(s)	71, 356, 661
BseXI	GCAGC	4 cut(s)	870, 925, 1034, 1133
BseYI	CCCAGC	1 cut(s)	742
BshFI	GGCC	2 cut(s)	236, 262
BsiSI	CCGG	3 cut(s)	524, 607, 905
BslFI	GGGAC	1 cut(s)	292
BslI	CCNNNNNNNGG	4 cut(s)	133, 134, 419, 1090
BsmAI	GTCTC	2 cut(s)	132, 646
BsmFI	GGGAC	1 cut(s)	292
BsmI	GAATGC	1 cut(s)	886
BsnI	GGCC	2 cut(s)	236, 262
Bsp13I	TCCGGA	1 cut(s)	523
Bsp143I	GATC	4 cut(s)	481, 511, 824, 1174
BspACI	CCGC	3 cut(s)	233, 326, 671
BspANI	GGCC	2 cut(s)	236, 262
BspCNI	CTCAG	7 cut(s)	266, 544, 573, 658, 685, 820, 1172
BspEI	TCCGGA	1 cut(s)	523
BspMI	ACCTGC	1 cut(s)	922
BspPI	GGATC	1 cut(s)	506
BsrDI	GCAATG	4 cut(s)	471, 890, 1147, 1170
BsrI	ACTGG	3 cut(s)	71, 356, 661
BssECI	CCNNGG	1 cut(s)	981
BssMI	GATC	4 cut(s)	481, 511, 824, 1174
Bst2UI	CCWGG	1 cut(s)	983
Bst4CI	ACNGT	4 cut(s)	37, 115, 598, 1001
Bst6I	CTCTTC	1 cut(s)	499
BstAPI	GCANNNNNTGC	1 cut(s)	892
BstC8I	GCNNGC	3 cut(s)	93, 264, 1033
BstDEI	CTNAG	7 cut(s)	253, 531, 560, 645, 693, 828, 1159
BstEII	GGTNACC	1 cut(s)	122
BstF5I	GGATG	4 cut(s)	10, 372, 722, 913
BstHHI	GCGC	2 cut(s)	97, 687
BstKTI	GATC	4 cut(s)	484, 514, 827, 1177
BstMAI	GTCTC	2 cut(s)	132, 646
BstMBI	GATC	4 cut(s)	481, 511, 824, 1174
BstMWI	GCNNNNNNNGC	2 cut(s)	892, 1121
BstNI	CCWGG	1 cut(s)	983
BstPI	GGTNACC	1 cut(s)	122
BstSCI	CCNGG	1 cut(s)	981
BstV1I	GCAGC	4 cut(s)	870, 925, 1034, 1133
BsuRI	GGCC	2 cut(s)	236, 262
BtsCI	GGATG	4 cut(s)	10, 372, 722, 913
BtsI	GCAGTG	1 cut(s)	516
BtsIMutI	CAGTG	3 cut(s)	78, 156, 516
BveI	ACCTGC	1 cut(s)	922
Cac8I	GCNNGC	3 cut(s)	93, 264, 1033
CaiI	CAGNNNCTG	1 cut(s)	538
CfoI	GCGC	2 cut(s)	97, 687
Cfr13I	GGNCC	1 cut(s)	260
CseI	GACGC	1 cut(s)	277
CviAII	CATG	6 cut(s)	28, 197, 317, 472, 611, 1036
DdeI	CTNAG	7 cut(s)	253, 531, 560, 645, 693, 828, 1159
DpnI	GATC	4 cut(s)	483, 513, 826, 1176
DpnII	GATC	4 cut(s)	481, 511, 824, 1174
DraI	TTTAAA	1 cut(s)	996
EaeI	YGGCCR	1 cut(s)	234
Eam1104I	CTCTTC	1 cut(s)	499
EarI	CTCTTC	1 cut(s)	499
Eco32I	GATATC	1 cut(s)	553
Eco57I	CTGAAG	2 cut(s)	560, 1040
Eco91I	GGTNACC	1 cut(s)	122
EcoO65I	GGTNACC	1 cut(s)	122
EcoRII	CCWGG	1 cut(s)	981
EcoRV	GATATC	1 cut(s)	553
FaeI	CATG	6 cut(s)	31, 200, 320, 475, 614, 1039
FaqI	GGGAC	1 cut(s)	292
FatI	CATG	6 cut(s)	27, 196, 316, 471, 610, 1035
FbaI	TGATCA	1 cut(s)	481
FblI	GTMKAC	1 cut(s)	203
Fnu4HI	GCNGC	5 cut(s)	234, 884, 914, 1048, 1122
FokI	GGATG	4 cut(s)	17, 359, 709, 920
Fsp4HI	GCNGC	5 cut(s)	234, 884, 914, 1048, 1122
FspI	TGCGCA	1 cut(s)	686
GlaI	GCGC	2 cut(s)	96, 686
GluI	GCNGC	5 cut(s)	234, 884, 914, 1048, 1122
GsaI	CCCAGC	1 cut(s)	746
GsuI	CTGGAG	1 cut(s)	678
HaeIII	GGCC	2 cut(s)	236, 262
HapII	CCGG	3 cut(s)	524, 607, 905
HgaI	GACGC	1 cut(s)	277
HhaI	GCGC	2 cut(s)	97, 687
Hin1II	CATG	6 cut(s)	31, 200, 320, 475, 614, 1039
Hin6I	GCGC	2 cut(s)	95, 685
HinP1I	GCGC	2 cut(s)	95, 685
HincII	GTYRAC	2 cut(s)	40, 976
HindII	GTYRAC	2 cut(s)	40, 976
HindIII	AAGCTT	1 cut(s)	866
HinfI	GANTC	4 cut(s)	181, 527, 573, 697
HpaI	GTTAAC	1 cut(s)	976
HpaII	CCGG	3 cut(s)	524, 607, 905
HphI	GGTGA	2 cut(s)	60, 894
Hpy166II	GTNNAC	3 cut(s)	40, 204, 976
Hpy188I	TCNGA	9 cut(s)	422, 486, 540, 550, 648, 829, 1103, 1171, 1179
Hpy188III	TCNNGA	7 cut(s)	101, 171, 246, 449, 524, 577, 1130
Hpy8I	GTNNAC	3 cut(s)	40, 204, 976
Hpy99I	CGWCG	2 cut(s)	293, 923
HpyAV	CCTTC	3 cut(s)	53, 481, 777
HpyCH4III	ACNGT	4 cut(s)	37, 115, 598, 1001
HpyCH4V	TGCA	7 cut(s)	223, 367, 464, 722, 785, 886, 1152
HpyF10VI	GCNNNNNNNGC	2 cut(s)	892, 1121
HpyF3I	CTNAG	7 cut(s)	253, 531, 560, 645, 693, 828, 1159
Hsp92II	CATG	6 cut(s)	31, 200, 320, 475, 614, 1039
HspAI	GCGC	2 cut(s)	95, 685
Kpn2I	TCCGGA	1 cut(s)	523
Ksp22I	TGATCA	1 cut(s)	481
KspAI	GTTAAC	1 cut(s)	976
Kzo9I	GATC	4 cut(s)	481, 511, 824, 1174
LmnI	GCTCC	3 cut(s)	395, 526, 862
Lsp1109I	GCAGC	4 cut(s)	870, 925, 1034, 1133
LweI	GCATC	4 cut(s)	94, 220, 313, 731
MaeIII	GTNAC	6 cut(s)	66, 109, 122, 151, 168, 632
MalI	GATC	4 cut(s)	483, 513, 826, 1176
MboI	GATC	4 cut(s)	481, 511, 824, 1174
MboII	GAAGA	8 cut(s)	332, 400, 448, 516, 812, 977, 1024, 1033
MfeI	CAATTG	1 cut(s)	129
MlyI	GAGTC	1 cut(s)	582
MnlI	CCTC	5 cut(s)	8, 774, 1045, 1109, 1168
MroI	TCCGGA	1 cut(s)	523
MseI	TTAA	5 cut(s)	270, 312, 675, 975, 995
MslI	CAYNNNNRTG	1 cut(s)	321
MspA1I	CMGCKG	1 cut(s)	671
MspI	CCGG	3 cut(s)	524, 607, 905
MspR9I	CCNGG	1 cut(s)	983
MunI	CAATTG	1 cut(s)	129
Mva1269I	GAATGC	1 cut(s)	886
MvaI	CCWGG	1 cut(s)	983
MwoI	GCNNNNNNNGC	2 cut(s)	892, 1121
NdeII	GATC	4 cut(s)	481, 511, 824, 1174
NlaIII	CATG	6 cut(s)	31, 200, 320, 475, 614, 1039
NmuCI	GTSAC	3 cut(s)	66, 151, 168
NsbI	TGCGCA	1 cut(s)	686
PaqCI	CACCTGC	1 cut(s)	922
PctI	GAATGC	1 cut(s)	886
PfeI	GAWTC	3 cut(s)	181, 527, 697
PflMI	CCANNNNNTGG	1 cut(s)	1090
PkrI	GCNGC	5 cut(s)	235, 885, 915, 1049, 1123
PleI	GAGTC	1 cut(s)	581
PpsI	GAGTC	1 cut(s)	581
Psp6I	CCWGG	1 cut(s)	981
PspEI	GGTNACC	1 cut(s)	122
PspFI	CCCAGC	1 cut(s)	742
PspGI	CCWGG	1 cut(s)	981
PspPI	GGNCC	1 cut(s)	260
PstNI	CAGNNNCTG	1 cut(s)	538
RseI	CAYNNNNRTG	1 cut(s)	321
SaqAI	TTAA	5 cut(s)	270, 312, 675, 975, 995
SatI	GCNGC	5 cut(s)	234, 884, 914, 1048, 1122
Sau3AI	GATC	4 cut(s)	481, 511, 824, 1174
Sau96I	GGNCC	1 cut(s)	260
SchI	GAGTC	1 cut(s)	582
ScrFI	CCNGG	1 cut(s)	983
SfaNI	GCATC	4 cut(s)	94, 220, 313, 731
SmiMI	CAYNNNNRTG	1 cut(s)	321
SsiI	CCGC	3 cut(s)	233, 326, 671
SspI	AATATT	1 cut(s)	334
StyD4I	CCNGG	1 cut(s)	981
TaaI	ACNGT	4 cut(s)	37, 115, 598, 1001
TaqI	TCGA	1 cut(s)	823
TauI	GCSGC	1 cut(s)	236
TfiI	GAWTC	3 cut(s)	181, 527, 697
Tru1I	TTAA	5 cut(s)	270, 312, 675, 975, 995
Tru9I	TTAA	5 cut(s)	270, 312, 675, 975, 995
TscAI	CASTG	3 cut(s)	78, 156, 523
TseFI	GTSAC	3 cut(s)	66, 151, 168
TseI	GCWGC	4 cut(s)	883, 913, 1047, 1121
Tsp45I	GTSAC	3 cut(s)	66, 151, 168
TspDTI	ATGAA	8 cut(s)	185, 333, 361, 488, 759, 861, 879, 978
TspRI	CASTG	3 cut(s)	78, 156, 523
Van91I	CCANNNNNTGG	1 cut(s)	1090
XapI	RAATTY	3 cut(s)	848, 1058, 1067
XmiI	GTMKAC	1 cut(s)	203

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.