protease Do-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1193 |
| Acc36I | ACCTGC | 1 cut(s) | 1480 |
| AccB1I | GGYRCC | 2 cut(s) | 292, 515 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 926, 1597 |
| AccI | GTMKAC | 1 cut(s) | 710 |
| AccII | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| AclWI | GGATC | 4 cut(s) | 251, 264, 1013, 1326 |
| AcoI | YGGCCR | 5 cut(s) | 181, 187, 246, 495, 1536 |
| AcsI | RAATTY | 2 cut(s) | 343, 1574 |
| AcyI | GRCGYC | 1 cut(s) | 126 |
| AdeI | CACNNNGTG | 2 cut(s) | 439, 522 |
| AfaI | GTAC | 3 cut(s) | 377, 491, 967 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 179, 641, 926, 1597 |
| AgeI | ACCGGT | 1 cut(s) | 577 |
| AgsI | TTSAA | 4 cut(s) | 1048, 1361, 1573, 1687 |
| AjnI | CCWGG | 2 cut(s) | 269, 453 |
| Alw21I | GWGCWC | 1 cut(s) | 423 |
| Alw26I | GTCTC | 1 cut(s) | 639 |
| AlwI | GGATC | 4 cut(s) | 251, 264, 1013, 1326 |
| Ama87I | CYCGRG | 2 cut(s) | 382, 760 |
| AoxI | GGCC | 8 cut(s) | 25, 181, 187, 246, 267, 495, 767, 1536 |
| ApeKI | GCWGC | 7 cut(s) | 352, 361, 387, 740, 1390, 1420, 1628 |
| ApoI | RAATTY | 2 cut(s) | 343, 1574 |
| AsiGI | ACCGGT | 1 cut(s) | 577 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 320 |
| AspLEI | GCGC | 2 cut(s) | 474, 1194 |
| AspS9I | GGNCC | 1 cut(s) | 767 |
| AsuC2I | CCSGG | 1 cut(s) | 245 |
| AsuHPI | GGTGA | 4 cut(s) | 325, 415, 567, 1401 |
| AvaI | CYCGRG | 2 cut(s) | 382, 760 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1296, 1329 |
| BalI | TGGCCA | 2 cut(s) | 497, 1538 |
| BamHI | GGATCC | 1 cut(s) | 256 |
| BanI | GGYRCC | 2 cut(s) | 292, 515 |
| BbsI | GAAGAC | 1 cut(s) | 1479 |
| Bbv12I | GWGCWC | 1 cut(s) | 423 |
| BbvI | GCAGC | 7 cut(s) | 339, 373, 399, 727, 1377, 1432, 1640 |
| BccI | CCATC | 5 cut(s) | 295, 1060, 1070, 1091, 1591 |
| BceAI | ACGGC | 6 cut(s) | 12, 113, 238, 280, 282, 340 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 274, 308, 700, 734 |
| BciT130I | CCWGG | 2 cut(s) | 271, 455 |
| BclI | TGATCA | 1 cut(s) | 988 |
| BcnI | CCSGG | 1 cut(s) | 245 |
| BcoDI | GTCTC | 1 cut(s) | 639 |
| BfaI | CTAG | 3 cut(s) | 59, 722, 1637 |
| BfuAI | ACCTGC | 1 cut(s) | 1480 |
| BmcAI | AGTACT | 1 cut(s) | 491 |
| Bme1390I | CCNGG | 3 cut(s) | 245, 271, 455 |
| BmeT110I | CYCGRG | 2 cut(s) | 382, 760 |
| BmgT120I | GGNCC | 1 cut(s) | 767 |
| BmiI | GGNNCC | 4 cut(s) | 258, 294, 478, 517 |
| BmrFI | CCNGG | 3 cut(s) | 245, 271, 455 |
| BmrI | ACTGGG | 1 cut(s) | 1173 |
| BmsI | GCATC | 6 cut(s) | 83, 525, 601, 727, 820, 1238 |
| BmuI | ACTGGG | 1 cut(s) | 1173 |
| BpiI | GAAGAC | 1 cut(s) | 1479 |
| BpuEI | CTTGAG | 1 cut(s) | 449 |
| BpuMI | CCSGG | 1 cut(s) | 245 |
| BsaBI | GATNNNNATC | 1 cut(s) | 255 |
| BsaHI | GRCGYC | 1 cut(s) | 126 |
| BsaJI | CCNNGG | 6 cut(s) | 178, 184, 243, 270, 356, 453 |
| BsaWI | WCCGGW | 2 cut(s) | 577, 1411 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 179, 641, 926, 1597 |
| Bse118I | RCCGGY | 1 cut(s) | 577 |
| Bse1I | ACTGG | 2 cut(s) | 863, 1168 |
| Bse3DI | GCAATG | 2 cut(s) | 528, 1677 |
| Bse8I | GATNNNNATC | 1 cut(s) | 255 |
| BseBI | CCWGG | 2 cut(s) | 271, 455 |
| BseDI | CCNNGG | 6 cut(s) | 178, 184, 243, 270, 356, 453 |
| BseGI | GGATG | 6 cut(s) | 74, 101, 835, 1229, 1420, 1567 |
| BseJI | GATNNNNATC | 1 cut(s) | 255 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 179, 641, 926, 1597 |
| BseMI | GCAATG | 2 cut(s) | 528, 1677 |
| BseMII | CTCAG | 7 cut(s) | 150, 450, 1052, 1081, 1191, 1222, 1451 |
| BseNI | ACTGG | 2 cut(s) | 863, 1168 |
| BseX3I | CGGCCG | 3 cut(s) | 181, 187, 246 |
| BseXI | GCAGC | 7 cut(s) | 339, 373, 399, 727, 1377, 1432, 1640 |
| BsgI | GTGCAG | 1 cut(s) | 310 |
| Bsh1236I | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| Bsh1285I | CGRYCG | 3 cut(s) | 184, 190, 249 |
| BshFI | GGCC | 8 cut(s) | 27, 183, 189, 248, 269, 497, 769, 1538 |
| BshNI | GGYRCC | 2 cut(s) | 292, 515 |
| BshTI | ACCGGT | 1 cut(s) | 577 |
| BsiEI | CGRYCG | 3 cut(s) | 184, 190, 249 |
| BsiHKAI | GWGCWC | 1 cut(s) | 423 |
| BsiHKCI | CYCGRG | 2 cut(s) | 382, 760 |
| BsiSI | CCGG | 3 cut(s) | 245, 578, 1412 |
| BslFI | GGGAC | 1 cut(s) | 799 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 179, 641, 926, 1597 |
| BsmAI | GTCTC | 1 cut(s) | 639 |
| BsmFI | GGGAC | 1 cut(s) | 799 |
| BsmI | GAATGC | 1 cut(s) | 1393 |
| BsnI | GGCC | 8 cut(s) | 27, 183, 189, 248, 269, 497, 769, 1538 |
| BsoBI | CYCGRG | 2 cut(s) | 382, 760 |
| Bsp1286I | GDGCHC | 1 cut(s) | 423 |
| Bsp143I | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| BspANI | GGCC | 8 cut(s) | 27, 183, 189, 248, 269, 497, 769, 1538 |
| BspCNI | CTCAG | 7 cut(s) | 149, 449, 1051, 1080, 1192, 1221, 1450 |
| BspFNI | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| BspLI | GGNNCC | 4 cut(s) | 258, 294, 478, 517 |
| BspMI | ACCTGC | 1 cut(s) | 1480 |
| BspPI | GGATC | 4 cut(s) | 251, 264, 1013, 1326 |
| BspT107I | GGYRCC | 2 cut(s) | 292, 515 |
| BsrDI | GCAATG | 2 cut(s) | 528, 1677 |
| BsrFI | RCCGGY | 1 cut(s) | 577 |
| BsrI | ACTGG | 2 cut(s) | 863, 1168 |
| BssAI | RCCGGY | 1 cut(s) | 577 |
| BssECI | CCNNGG | 6 cut(s) | 178, 184, 243, 270, 356, 453 |
| BssMI | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| BssNI | GRCGYC | 1 cut(s) | 126 |
| Bst2UI | CCWGG | 2 cut(s) | 271, 455 |
| Bst4CI | ACNGT | 6 cut(s) | 502, 544, 622, 1105, 1556, 1665 |
| BstACI | GRCGYC | 1 cut(s) | 126 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1399 |
| BstC8I | GCNNGC | 5 cut(s) | 240, 329, 600, 771, 1540 |
| BstDEI | CTNAG | 7 cut(s) | 136, 436, 1038, 1067, 1200, 1208, 1437 |
| BstDSI | CCRYGG | 3 cut(s) | 178, 184, 356 |
| BstF5I | GGATG | 6 cut(s) | 74, 101, 835, 1229, 1420, 1567 |
| BstFNI | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| BstHHI | GCGC | 2 cut(s) | 474, 1194 |
| BstKTI | GATC | 5 cut(s) | 259, 747, 991, 1021, 1334 |
| BstMAI | GTCTC | 1 cut(s) | 639 |
| BstMBI | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| BstMCI | CGRYCG | 3 cut(s) | 184, 190, 249 |
| BstNI | CCWGG | 2 cut(s) | 271, 455 |
| BstSCI | CCNGG | 3 cut(s) | 243, 269, 453 |
| BstUI | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| BstV1I | GCAGC | 7 cut(s) | 339, 373, 399, 727, 1377, 1432, 1640 |
| BstV2I | GAAGAC | 1 cut(s) | 1479 |
| BstX2I | RGATCY | 1 cut(s) | 256 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 493, 1030 |
| BstYI | RGATCY | 1 cut(s) | 256 |
| BstZI | CGGCCG | 3 cut(s) | 181, 187, 246 |
| BsuRI | GGCC | 8 cut(s) | 27, 183, 189, 248, 269, 497, 769, 1538 |
| BtgI | CCRYGG | 3 cut(s) | 178, 184, 356 |
| BtgZI | GCGATG | 1 cut(s) | 548 |
| BtsCI | GGATG | 6 cut(s) | 74, 101, 835, 1229, 1420, 1567 |
| BtsI | GCAGTG | 3 cut(s) | 397, 1023, 1654 |
| BtsIMutI | CAGTG | 6 cut(s) | 397, 444, 498, 663, 1023, 1654 |
| BveI | ACCTGC | 1 cut(s) | 1480 |
| Cac8I | GCNNGC | 5 cut(s) | 240, 329, 600, 771, 1540 |
| CciNI | GCGGCCGC | 2 cut(s) | 181, 187 |
| CfoI | GCGC | 2 cut(s) | 474, 1194 |
| Cfr10I | RCCGGY | 1 cut(s) | 577 |
| Cfr13I | GGNCC | 1 cut(s) | 767 |
| Cfr42I | CCGCGG | 2 cut(s) | 181, 187 |
| CseI | GACGC | 2 cut(s) | 134, 314 |
| Csp6I | GTAC | 3 cut(s) | 376, 490, 966 |
| CspAI | ACCGGT | 1 cut(s) | 577 |
| CviAII | CATG | 5 cut(s) | 704, 824, 979, 1118, 1543 |
| CviQI | GTAC | 3 cut(s) | 376, 490, 966 |
| DdeI | CTNAG | 7 cut(s) | 136, 436, 1038, 1067, 1200, 1208, 1437 |
| DpnI | GATC | 5 cut(s) | 258, 746, 990, 1020, 1333 |
| DpnII | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| DraIII | CACNNNGTG | 2 cut(s) | 439, 522 |
| EaeI | YGGCCR | 5 cut(s) | 181, 187, 246, 495, 1536 |
| EagI | CGGCCG | 3 cut(s) | 181, 187, 246 |
| EciI | GGCGGA | 1 cut(s) | 94 |
| EclXI | CGGCCG | 3 cut(s) | 181, 187, 246 |
| Eco32I | GATATC | 1 cut(s) | 1456 |
| Eco52I | CGGCCG | 3 cut(s) | 181, 187, 246 |
| Eco88I | CYCGRG | 2 cut(s) | 382, 760 |
| EcoRII | CCWGG | 2 cut(s) | 269, 453 |
| EcoRV | GATATC | 1 cut(s) | 1456 |
| FaeI | CATG | 5 cut(s) | 707, 827, 982, 1121, 1546 |
| FaqI | GGGAC | 1 cut(s) | 799 |
| FatI | CATG | 5 cut(s) | 703, 823, 978, 1117, 1542 |
| FauI | CCCGC | 6 cut(s) | 182, 243, 247, 268, 272, 607 |
| FbaI | TGATCA | 1 cut(s) | 988 |
| FblI | GTMKAC | 1 cut(s) | 710 |
| FokI | GGATG | 6 cut(s) | 61, 88, 842, 1216, 1427, 1574 |
| FspBI | CTAG | 3 cut(s) | 59, 722, 1637 |
| FspI | TGCGCA | 1 cut(s) | 1193 |
| GlaI | GCGC | 2 cut(s) | 473, 1193 |
| HaeIII | GGCC | 8 cut(s) | 27, 183, 189, 248, 269, 497, 769, 1538 |
| HapII | CCGG | 3 cut(s) | 245, 578, 1412 |
| HgaI | GACGC | 2 cut(s) | 134, 314 |
| HhaI | GCGC | 2 cut(s) | 474, 1194 |
| Hin1I | GRCGYC | 1 cut(s) | 126 |
| Hin1II | CATG | 5 cut(s) | 707, 827, 982, 1121, 1546 |
| Hin6I | GCGC | 2 cut(s) | 472, 1192 |
| HinP1I | GCGC | 2 cut(s) | 472, 1192 |
| HincII | GTYRAC | 2 cut(s) | 711, 1483 |
| HindII | GTYRAC | 2 cut(s) | 711, 1483 |
| HindIII | AAGCTT | 1 cut(s) | 1531 |
| HinfI | GANTC | 5 cut(s) | 251, 688, 1034, 1204, 1522 |
| HpaI | GTTAAC | 1 cut(s) | 1483 |
| HpaII | CCGG | 3 cut(s) | 245, 578, 1412 |
| HphI | GGTGA | 4 cut(s) | 325, 415, 567, 1401 |
| Hpy166II | GTNNAC | 3 cut(s) | 32, 711, 1483 |
| Hpy188III | TCNNGA | 6 cut(s) | 151, 205, 466, 753, 956, 1031 |
| Hpy8I | GTNNAC | 3 cut(s) | 32, 711, 1483 |
| Hpy99I | CGWCG | 4 cut(s) | 119, 128, 152, 301 |
| HpyAV | CCTTC | 3 cut(s) | 310, 560, 1284 |
| HpyCH4III | ACNGT | 6 cut(s) | 502, 544, 622, 1105, 1556, 1665 |
| HpyCH4IV | ACGT | 1 cut(s) | 147 |
| HpyF3I | CTNAG | 7 cut(s) | 136, 436, 1038, 1067, 1200, 1208, 1437 |
| HpySE526I | ACGT | 1 cut(s) | 147 |
| Hsp92I | GRCGYC | 1 cut(s) | 126 |
| Hsp92II | CATG | 5 cut(s) | 707, 827, 982, 1121, 1546 |
| HspAI | GCGC | 2 cut(s) | 472, 1192 |
| Ksp22I | TGATCA | 1 cut(s) | 988 |
| KspAI | GTTAAC | 1 cut(s) | 1483 |
| KspI | CCGCGG | 2 cut(s) | 181, 187 |
| Kzo9I | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| LmnI | GCTCC | 5 cut(s) | 207, 442, 482, 902, 1369 |
| Lsp1109I | GCAGC | 7 cut(s) | 339, 373, 399, 727, 1377, 1432, 1640 |
| LweI | GCATC | 6 cut(s) | 83, 525, 601, 727, 820, 1238 |
| MaeI | CTAG | 3 cut(s) | 59, 722, 1637 |
| MaeII | ACGT | 1 cut(s) | 147 |
| MaeIII | GTNAC | 6 cut(s) | 524, 573, 616, 658, 1139, 1407 |
| MalI | GATC | 5 cut(s) | 258, 746, 990, 1020, 1333 |
| MboI | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| MboII | GAAGA | 6 cut(s) | 907, 1023, 1055, 1319, 1484, 1531 |
| MflI | RGATCY | 1 cut(s) | 256 |
| MhlI | GDGCHC | 1 cut(s) | 423 |
| MlsI | TGGCCA | 2 cut(s) | 497, 1538 |
| MluCI | AATT | 6 cut(s) | 343, 921, 1014, 1195, 1245, 1574 |
| MluNI | TGGCCA | 2 cut(s) | 497, 1538 |
| Mox20I | TGGCCA | 2 cut(s) | 497, 1538 |
| MroXI | GAANNNNTTC | 1 cut(s) | 320 |
| MscI | TGGCCA | 2 cut(s) | 497, 1538 |
| MseI | TTAA | 5 cut(s) | 777, 1083, 1182, 1482, 1503 |
| MslI | CAYNNNNRTG | 2 cut(s) | 533, 828 |
| Msp20I | TGGCCA | 2 cut(s) | 497, 1538 |
| MspA1I | CMGCKG | 3 cut(s) | 180, 186, 1310 |
| MspI | CCGG | 3 cut(s) | 245, 578, 1412 |
| MspR9I | CCNGG | 3 cut(s) | 245, 271, 455 |
| Mva1269I | GAATGC | 1 cut(s) | 1393 |
| MvaI | CCWGG | 2 cut(s) | 271, 455 |
| MvnI | CGCG | 5 cut(s) | 180, 186, 308, 474, 679 |
| NciI | CCSGG | 1 cut(s) | 245 |
| NdeII | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| NlaIII | CATG | 5 cut(s) | 707, 827, 982, 1121, 1546 |
| NlaIV | GGNNCC | 4 cut(s) | 258, 294, 478, 517 |
| NmuCI | GTSAC | 4 cut(s) | 524, 573, 658, 1407 |
| NotI | GCGGCCGC | 2 cut(s) | 181, 187 |
| NsbI | TGCGCA | 1 cut(s) | 1193 |
| PaeR7I | CTCGAG | 1 cut(s) | 382 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 120, 156, 228 |
| PctI | GAATGC | 1 cut(s) | 1393 |
| PdmI | GAANNNNTTC | 1 cut(s) | 320 |
| PfeI | GAWTC | 5 cut(s) | 251, 688, 1034, 1204, 1522 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 926, 1597 |
| PinAI | ACCGGT | 1 cut(s) | 577 |
| Psp6I | CCWGG | 2 cut(s) | 269, 453 |
| PspGI | CCWGG | 2 cut(s) | 269, 453 |
| PspN4I | GGNNCC | 4 cut(s) | 258, 294, 478, 517 |
| PspPI | GGNCC | 1 cut(s) | 767 |
| PspXI | VCTCGAGB | 1 cut(s) | 382 |
| PsuI | RGATCY | 1 cut(s) | 256 |
| PvuII | CAGCTG | 1 cut(s) | 1310 |
| RsaI | GTAC | 3 cut(s) | 377, 491, 967 |
| RsaNI | GTAC | 3 cut(s) | 376, 490, 966 |
| RseI | CAYNNNNRTG | 2 cut(s) | 533, 828 |
| SacII | CCGCGG | 2 cut(s) | 181, 187 |
| SalI | GTCGAC | 1 cut(s) | 709 |
| SaqAI | TTAA | 5 cut(s) | 777, 1083, 1182, 1482, 1503 |
| Sau3AI | GATC | 5 cut(s) | 256, 744, 988, 1018, 1331 |
| Sau96I | GGNCC | 1 cut(s) | 767 |
| ScaI | AGTACT | 1 cut(s) | 491 |
| ScrFI | CCNGG | 3 cut(s) | 245, 271, 455 |
| SduI | GDGCHC | 1 cut(s) | 423 |
| SfaNI | GCATC | 6 cut(s) | 83, 525, 601, 727, 820, 1238 |
| Sfr274I | CTCGAG | 1 cut(s) | 382 |
| Sfr303I | CCGCGG | 2 cut(s) | 181, 187 |
| SgrAI | CRCCGGYG | 1 cut(s) | 577 |
| SgrBI | CCGCGG | 2 cut(s) | 181, 187 |
| SlaI | CTCGAG | 1 cut(s) | 382 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 533, 828 |
| SmlI | CTYRAG | 2 cut(s) | 382, 464 |
| SmoI | CTYRAG | 2 cut(s) | 382, 464 |
| Sse9I | AATT | 6 cut(s) | 343, 921, 1014, 1195, 1245, 1574 |
| SspI | AATATT | 2 cut(s) | 841, 1087 |
| SspMI | CTAG | 3 cut(s) | 59, 722, 1637 |
| StyD4I | CCNGG | 3 cut(s) | 243, 269, 453 |
| TaaI | ACNGT | 6 cut(s) | 502, 544, 622, 1105, 1556, 1665 |
| TaiI | ACGT | 1 cut(s) | 150 |
| TaqI | TCGA | 6 cut(s) | 150, 159, 299, 383, 710, 1330 |
| TasI | AATT | 6 cut(s) | 343, 921, 1014, 1195, 1245, 1574 |
| TatI | WGTACW | 2 cut(s) | 375, 489 |
| TauI | GCSGC | 9 cut(s) | 110, 180, 183, 186, 189, 192, 411, 477, 1405 |
| TfiI | GAWTC | 5 cut(s) | 251, 688, 1034, 1204, 1522 |
| Tru1I | TTAA | 5 cut(s) | 777, 1083, 1182, 1482, 1503 |
| Tru9I | TTAA | 5 cut(s) | 777, 1083, 1182, 1482, 1503 |
| TscAI | CASTG | 6 cut(s) | 397, 444, 505, 663, 1030, 1661 |
| TseFI | GTSAC | 4 cut(s) | 524, 573, 658, 1407 |
| TseI | GCWGC | 7 cut(s) | 352, 361, 387, 740, 1390, 1420, 1628 |
| Tsp45I | GTSAC | 4 cut(s) | 524, 573, 658, 1407 |
| TspDTI | ATGAA | 5 cut(s) | 692, 868, 995, 1266, 1485 |
| TspRI | CASTG | 6 cut(s) | 397, 444, 505, 663, 1030, 1661 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 926, 1597 |
| XapI | RAATTY | 2 cut(s) | 343, 1574 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1160 |
| XhoI | CTCGAG | 1 cut(s) | 382 |
| XmiI | GTMKAC | 1 cut(s) | 710 |
| XmnI | GAANNNNTTC | 1 cut(s) | 320 |
| XspI | CTAG | 3 cut(s) | 59, 722, 1637 |
| ZrmI | AGTACT | 1 cut(s) | 491 |