Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Reverse (-)

2796340 .. 2800037

Gene Structure 3698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1038500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGGCGAAAATAAAAAAAGAAAAAGAGGTCGAAAACCCAAAACCCCAGAAGCCGATGACACCAATATCACCACCATGGACATCCAATACACCAACCCCACCGCCGTGGACGACACTGTGTCAGTTCCGGCGAGCGAAACCACCTCGGACCCAGGCAGCCCCCAGCCCGCCCGCCGCGGTAGAGGGCGGCCCAGAAAGACCGAGAACCCCAACATGCCGGGTTCGCCGGAACGGCGGAGCTCGAGACACATGGATCAGAACGGCGATGGTGGGCACGCCTTGGCAGTCGTTTCGGAGCCGCCGATGATGGTGCCGGGCCGGGAGAGCGTGGAGAGGGTGGTCCCCGCCATGGACGCCGTCGTGAAGGTGTTCTGTGTCCACACGGAGCCGAATTTCTCGCTGCCGTGGCAGCGGAAGCGGCAGTACAGTTCGAGCAGTAGTGGGTTTGTGATTGGGGGACGGAGGGTGCTGACCAATGCGCATTCTGTGGAGCACCATACGCAGGTGAAGCTCAAGAAACGCGGCTCTGAGACGAAGTACTTGGCCACTGTGTTAGCCATAGGCACCGAGTGTGATATCGCAATGCTTACTGTGAGCGATGATGAGTTCTGGAAAGGAGTTTCACCTCTGGAGTTTGGAGAGTTACCTGCACTCCAAGATGCTGTGACAGTTGTGGGCTACCCTATTGGGGGTGACACGATCTCTGTGACAAGTGGTGTTGTGTCACGTATGGAGATACTGTCTTATGTTCATGGATCGACTGAGCTTCTAGGACTGCAGATAGACGCTGCAATAAACTCTGGAAACTCTGGTGGGCCTGCCTTTAATGATAAGGGAGAATGCGTGGGTATTGCATTTCAGTCTCTTAAACATGAAGACGCGGAAAATATAGGCTATGTCATACCGACACCAGTTATTAAGCACTTCATCCAAGATTATGAGAAGAATGGGGCATATACAGGGTTCCCAGTTCTTGGAATCGAATGGCAGAAGATGGAAAATCCAGATCTGCGCATCTCAATGGGGATGAGACTTGATCAAAAGGGTGTCCGTATTAGAAGAGTTGAACCTACAGCTCCACATTCTCATGTTCTGCAGCCATCTGATGTTATTCTTAGCTTTGATGGGGTTAATATTGCAAATGACGGCACAGTTCCATTCAGGCATGGAGAGCGCATAGGTTTCAGTTACCTTGTCTCTCAAAAATATACTGCCGATAGTGCTCTAGTAAAAGTTCTTCGTAACTCAGAGATACTTGAGTACAACATAAAACTTGGAACTCACAAGCGACTCATCCCAGCACACATTAAGGGTAAACCTCCTTCGTATTACATCGTTGGTGGTTTTGTTTTTGCAGCTGTATCTGTTCCATATCTGCGTTCTGAGTATGGAAAGGATTACGAATATGATGCTCCAGTCAAGCTGCTGGACAAACATTTACATGCAATGGCGGAGTCTATCGATGAACAGCTAGTTGTTGTTTCTCAGGTGCTTGTTGCTGACATTAACATTGGATACGAGGACATTGTGAACACTCAGGTTCTTGCTTTCAATGGTAAGCCTGTGAAAAATCTGAAGAGTCTGGTTAGCATGGTGGAGAATTGTGACGACGAGTACCTAAAGTTTGACCTAGAATACAATCAGATGGTTGTACTACAGACAAAAACCGCCAAGGCGTCAACTCTAGATATCCTCCTTACACATTGCATACCTTCAGCTATGTCTGATGACCTTAAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

581

Amino Acids

63.81

Weight (kDa)

6.21

Isoelectric Point (pI)

45.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	132 - 310	8.7e-20	Trypsin
Trypsin_2	PF13365	147 - 284	5.4e-21	Trypsin-like peptidase domain
PDZ_3	PF17815	432 - 577	1.4e-48	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	493
Acc16I	TGCGCA	2 cut(s)	480, 1013
Acc36I	ACCTGC	2 cut(s)	493, 655
AccB1I	GGYRCC	2 cut(s)	310, 563
AccB7I	CCANNNNNTGG	1 cut(s)	974
AccII	CGCG	3 cut(s)	177, 522, 881
AclWI	GGATC	2 cut(s)	261, 763
AcoI	YGGCCR	1 cut(s)	543
AcsI	RAATTY	1 cut(s)	391
AcuI	CTGAAG	2 cut(s)	1595, 1698
AcyI	GRCGYC	2 cut(s)	354, 1676
AdeI	CACNNNGTG	1 cut(s)	570
AfaI	GTAC	5 cut(s)	425, 539, 1262, 1616, 1653
AfiI	CCNNNNNNNGG	5 cut(s)	349, 502, 688, 689, 974
AflII	CTTAAG	1 cut(s)	1733
AgsI	TTSAA	2 cut(s)	1067, 1552
AhdI	GACNNNNNGTC	2 cut(s)	118, 1735
AjnI	CCWGG	1 cut(s)	151
AleI	CACNNNNGTG	1 cut(s)	104
AluBI	AGCT	9 cut(s)	240, 511, 766, 1076, 1119, 1358, 1423, 1471, 1718
AluI	AGCT	9 cut(s)	240, 511, 766, 1076, 1119, 1358, 1423, 1471, 1718
Alw21I	GWGCWC	3 cut(s)	242, 495, 1225
Alw26I	GTCTC	5 cut(s)	238, 524, 867, 1024, 1201
AlwI	GGATC	2 cut(s)	261, 763
Ama87I	CYCGRG	1 cut(s)	241
AoxI	GGCC	4 cut(s)	188, 316, 543, 815
ApeKI	GCWGC	7 cut(s)	156, 400, 409, 788, 1096, 1355, 1423
ApoI	RAATTY	1 cut(s)	391
Asp700I	GAANNNNTTC	1 cut(s)	368
AspLEI	GCGC	3 cut(s)	481, 1014, 1176
AspS9I	GGNCC	5 cut(s)	148, 189, 316, 340, 815
AsuC2I	CCSGG	3 cut(s)	219, 315, 320
AsuHPI	GGTGA	4 cut(s)	61, 517, 615, 704
AvaI	CYCGRG	1 cut(s)	241
AvaII	GGWCC	2 cut(s)	148, 340
BaeGI	GKGCMC	1 cut(s)	276
BaeI	ACNNNNGTAYC	2 cut(s)	1244, 1277
BalI	TGGCCA	1 cut(s)	545
BanI	GGYRCC	2 cut(s)	310, 563
BanII	GRGCYC	1 cut(s)	242
BarI	GAAGNNNNNNTAC	6 cut(s)	407, 439, 1221, 1253, 1306, 1338
BbsI	GAAGAC	1 cut(s)	882
Bbv12I	GWGCWC	3 cut(s)	242, 495, 1225
BbvI	GCAGC	7 cut(s)	168, 387, 421, 775, 1108, 1367, 1410
BccI	CCATC	6 cut(s)	260, 301, 988, 1108, 1118, 1639
BceAI	ACGGC	6 cut(s)	89, 248, 277, 341, 388, 1162
BcgI	CGANNNNNNTGC	6 cut(s)	273, 292, 307, 326, 1391, 1425
BciT130I	CCWGG	1 cut(s)	153
BciVI	GTATCC	1 cut(s)	1508
BclI	TGATCA	1 cut(s)	1036
BcnI	CCSGG	3 cut(s)	219, 315, 320
BcoDI	GTCTC	5 cut(s)	238, 524, 867, 1024, 1201
BfaI	CTAG	5 cut(s)	770, 1226, 1472, 1631, 1685
BfmI	CTRYAG	4 cut(s)	776, 1071, 1094, 1655
BfrI	CTTAAG	1 cut(s)	1733
BfuAI	ACCTGC	2 cut(s)	493, 655
BfuI	GTATCC	1 cut(s)	1508
BglI	GCCNNNNNGGC	1 cut(s)	232
BglII	AGATCT	1 cut(s)	1006
BmcAI	AGTACT	1 cut(s)	539
Bme1390I	CCNGG	4 cut(s)	153, 219, 315, 320
Bme18I	GGWCC	2 cut(s)	148, 340
BmeRI	GACNNNNNGTC	2 cut(s)	118, 1735
BmeT110I	CYCGRG	1 cut(s)	241
BmgT120I	GGNCC	5 cut(s)	148, 189, 316, 340, 815
BmiI	GGNNCC	7 cut(s)	150, 297, 312, 342, 387, 565, 965
BmrFI	CCNGG	4 cut(s)	153, 219, 315, 320
BmrI	ACTGGG	1 cut(s)	962
BmsI	GCATC	3 cut(s)	649, 1023, 1399
BmuI	ACTGGG	1 cut(s)	962
BpiI	GAAGAC	1 cut(s)	882
BpmI	CTGGAG	2 cut(s)	650, 1398
BpuEI	CTTGAG	2 cut(s)	497, 1277
BpuMI	CCSGG	3 cut(s)	219, 315, 320
Bsa29I	ATCGAT	1 cut(s)	1461
BsaAI	YACGTR	1 cut(s)	728
BsaHI	GRCGYC	2 cut(s)	354, 1676
BsaJI	CCNNGG	9 cut(s)	75, 105, 144, 151, 175, 279, 348, 404, 1671
BsaXI	ACNNNNNCTCC	6 cut(s)	636, 666, 725, 755, 828, 858
Bsc4I	CCNNNNNNNGG	5 cut(s)	349, 502, 688, 689, 974
Bse1I	ACTGG	3 cut(s)	911, 968, 1415
Bse3DI	GCAATG	3 cut(s)	588, 1452, 1702
BseBI	CCWGG	1 cut(s)	153
BseCI	ATCGAT	1 cut(s)	1461
BseDI	CCNNGG	9 cut(s)	75, 105, 144, 151, 175, 279, 348, 404, 1671
BseGI	GGATG	4 cut(s)	81, 927, 1032, 1293
BseLI	CCNNNNNNNGG	5 cut(s)	349, 502, 688, 689, 974
BseMI	GCAATG	3 cut(s)	588, 1452, 1702
BseMII	CTCAG	6 cut(s)	519, 753, 1260, 1374, 1499, 1550
BseNI	ACTGG	3 cut(s)	911, 968, 1415
BseSI	GKGCMC	1 cut(s)	276
BseXI	GCAGC	7 cut(s)	168, 387, 421, 775, 1108, 1367, 1410
BseYI	CCCAGC	2 cut(s)	162, 1297
BsgI	GTGCAG	1 cut(s)	633
Bsh1236I	CGCG	3 cut(s)	177, 522, 881
BshFI	GGCC	4 cut(s)	190, 318, 545, 817
BshNI	GGYRCC	2 cut(s)	310, 563
BshVI	ATCGAT	1 cut(s)	1461
BsiHKAI	GWGCWC	3 cut(s)	242, 495, 1225
BsiHKCI	CYCGRG	1 cut(s)	241
BsiSI	CCGG	5 cut(s)	128, 218, 227, 314, 319
BslFI	GGGAC	2 cut(s)	326, 471
BslI	CCNNNNNNNGG	5 cut(s)	349, 502, 688, 689, 974
BsmAI	GTCTC	5 cut(s)	238, 524, 867, 1024, 1201
BsmBI	CGTCTC	1 cut(s)	524
BsmFI	GGGAC	2 cut(s)	326, 471
BsmI	GAATGC	2 cut(s)	481, 845
BsnI	GGCC	4 cut(s)	190, 318, 545, 817
BsoBI	CYCGRG	1 cut(s)	241
Bsp1286I	GDGCHC	4 cut(s)	242, 276, 495, 1225
Bsp143I	GATC	5 cut(s)	253, 699, 755, 1006, 1036
Bsp19I	CCATGG	2 cut(s)	75, 348
BspANI	GGCC	4 cut(s)	190, 318, 545, 817
BspCNI	CTCAG	6 cut(s)	520, 754, 1259, 1375, 1498, 1549
BspDI	ATCGAT	1 cut(s)	1461
BspFNI	CGCG	3 cut(s)	177, 522, 881
BspLI	GGNNCC	7 cut(s)	150, 297, 312, 342, 387, 565, 965
BspMAI	CTGCAG	2 cut(s)	780, 1098
BspMI	ACCTGC	2 cut(s)	493, 655
BspPI	GGATC	2 cut(s)	261, 763
BspT107I	GGYRCC	2 cut(s)	310, 563
BspTI	CTTAAG	1 cut(s)	1733
BsrDI	GCAATG	3 cut(s)	588, 1452, 1702
BsrI	ACTGG	3 cut(s)	911, 968, 1415
BssECI	CCNNGG	9 cut(s)	75, 105, 144, 151, 175, 279, 348, 404, 1671
BssMI	GATC	5 cut(s)	253, 699, 755, 1006, 1036
BssNI	GRCGYC	2 cut(s)	354, 1676
BssT1I	CCWWGG	4 cut(s)	75, 279, 348, 1671
Bst2UI	CCWGG	1 cut(s)	153
Bst4CI	ACNGT	7 cut(s)	118, 428, 550, 592, 670, 741, 1153
Bst6I	CTCTTC	2 cut(s)	1054, 1571
BstACI	GRCGYC	2 cut(s)	354, 1676
BstAFI	CTTAAG	1 cut(s)	1733
BstBAI	YACGTR	1 cut(s)	728
BstC8I	GCNNGC	5 cut(s)	133, 168, 172, 276, 819
BstDEI	CTNAG	7 cut(s)	528, 762, 1115, 1246, 1383, 1485, 1536
BstDSI	CCRYGG	5 cut(s)	75, 105, 175, 348, 404
BstF5I	GGATG	4 cut(s)	81, 927, 1032, 1293
BstFNI	CGCG	3 cut(s)	177, 522, 881
BstHHI	GCGC	3 cut(s)	481, 1014, 1176
BstKTI	GATC	5 cut(s)	256, 702, 758, 1009, 1039
BstMAI	GTCTC	5 cut(s)	238, 524, 867, 1024, 1201
BstMBI	GATC	5 cut(s)	253, 699, 755, 1006, 1036
BstNI	CCWGG	1 cut(s)	153
BstNSI	RCATGY	2 cut(s)	217, 1445
BstSCI	CCNGG	4 cut(s)	151, 217, 313, 318
BstSFI	CTRYAG	4 cut(s)	776, 1071, 1094, 1655
BstSLI	GKGCMC	1 cut(s)	276
BstUI	CGCG	3 cut(s)	177, 522, 881
BstV1I	GCAGC	7 cut(s)	168, 387, 421, 775, 1108, 1367, 1410
BstV2I	GAAGAC	1 cut(s)	882
BstX2I	RGATCY	1 cut(s)	1006
BstXI	CCANNNNNNTGG	1 cut(s)	106
BstYI	RGATCY	1 cut(s)	1006
Bsu15I	ATCGAT	1 cut(s)	1461
BsuI	GTATCC	1 cut(s)	1508
BsuRI	GGCC	4 cut(s)	190, 318, 545, 817
BsuTUI	ATCGAT	1 cut(s)	1461
BtgI	CCRYGG	5 cut(s)	75, 105, 175, 348, 404
BtgZI	GCGATG	2 cut(s)	279, 612
BtsCI	GGATG	4 cut(s)	81, 927, 1032, 1293
BtsIMutI	CAGTG	2 cut(s)	114, 546
BveI	ACCTGC	2 cut(s)	493, 655
Cac8I	GCNNGC	5 cut(s)	133, 168, 172, 276, 819
CfoI	GCGC	3 cut(s)	481, 1014, 1176
Cfr13I	GGNCC	5 cut(s)	148, 189, 316, 340, 815
Cfr42I	CCGCGG	1 cut(s)	178
ClaI	ATCGAT	1 cut(s)	1461
CseI	GACGC	4 cut(s)	362, 794, 887, 1665
Csp6I	GTAC	5 cut(s)	424, 538, 1261, 1615, 1652
CviQI	GTAC	5 cut(s)	424, 538, 1261, 1615, 1652
DdeI	CTNAG	7 cut(s)	528, 762, 1115, 1246, 1383, 1485, 1536
DpnI	GATC	5 cut(s)	255, 701, 757, 1008, 1038
DpnII	GATC	5 cut(s)	253, 699, 755, 1006, 1036
DraIII	CACNNNGTG	1 cut(s)	570
DriI	GACNNNNNGTC	2 cut(s)	118, 1735
EaeI	YGGCCR	1 cut(s)	543
Eam1104I	CTCTTC	2 cut(s)	1054, 1571
Eam1105I	GACNNNNNGTC	2 cut(s)	118, 1735
EarI	CTCTTC	2 cut(s)	1054, 1571
EciI	GGCGGA	2 cut(s)	250, 1466
Ecl136II	GAGCTC	1 cut(s)	240
Eco130I	CCWWGG	4 cut(s)	75, 279, 348, 1671
Eco24I	GRGCYC	1 cut(s)	242
Eco32I	GATATC	2 cut(s)	577, 1690
Eco47I	GGWCC	2 cut(s)	148, 340
Eco53kI	GAGCTC	1 cut(s)	240
Eco57I	CTGAAG	2 cut(s)	1595, 1698
Eco88I	CYCGRG	1 cut(s)	241
EcoICRI	GAGCTC	1 cut(s)	240
EcoRII	CCWGG	1 cut(s)	151
EcoRV	GATATC	2 cut(s)	577, 1690
EcoT14I	CCWWGG	4 cut(s)	75, 279, 348, 1671
EcoT38I	GRGCYC	1 cut(s)	242
ErhI	CCWWGG	4 cut(s)	75, 279, 348, 1671
Esp3I	CGTCTC	1 cut(s)	524
FaqI	GGGAC	2 cut(s)	326, 471
FauI	CCCGC	3 cut(s)	175, 179, 352
FbaI	TGATCA	1 cut(s)	1036
FokI	GGATG	4 cut(s)	68, 914, 1039, 1280
FriOI	GRGCYC	1 cut(s)	242
FspAI	RTGCGCAY	1 cut(s)	480
FspBI	CTAG	5 cut(s)	770, 1226, 1472, 1631, 1685
FspI	TGCGCA	2 cut(s)	480, 1013
GlaI	GCGC	3 cut(s)	480, 1013, 1175
GsaI	CCCAGC	2 cut(s)	166, 1301
GsuI	CTGGAG	2 cut(s)	650, 1398
HaeIII	GGCC	4 cut(s)	190, 318, 545, 817
HapII	CCGG	5 cut(s)	128, 218, 227, 314, 319
HgaI	GACGC	4 cut(s)	362, 794, 887, 1665
HhaI	GCGC	3 cut(s)	481, 1014, 1176
Hin1I	GRCGYC	2 cut(s)	354, 1676
Hin6I	GCGC	3 cut(s)	479, 1012, 1174
HinP1I	GCGC	3 cut(s)	479, 1012, 1174
HincII	GTYRAC	1 cut(s)	1680
HindII	GTYRAC	1 cut(s)	1680
HinfI	GANTC	4 cut(s)	978, 1290, 1454, 1579
HpaII	CCGG	5 cut(s)	128, 218, 227, 314, 319
HphI	GGTGA	4 cut(s)	61, 517, 615, 704
Hpy166II	GTNNAC	5 cut(s)	109, 379, 1316, 1531, 1680
Hpy188III	TCNNGA	8 cut(s)	243, 361, 514, 610, 629, 801, 1004, 1685
Hpy8I	GTNNAC	5 cut(s)	109, 379, 1316, 1531, 1680
Hpy99I	CGWCG	2 cut(s)	362, 1613
HpyAV	CCTTC	3 cut(s)	358, 1332, 1722
HpyCH4III	ACNGT	7 cut(s)	118, 428, 550, 592, 670, 741, 1153
HpyCH4IV	ACGT	1 cut(s)	727
HpyCH4V	TGCA	9 cut(s)	650, 778, 791, 854, 1096, 1139, 1355, 1445, 1707
HpyF3I	CTNAG	7 cut(s)	528, 762, 1115, 1246, 1383, 1485, 1536
HpySE526I	ACGT	1 cut(s)	727
Hsp92I	GRCGYC	2 cut(s)	354, 1676
HspAI	GCGC	3 cut(s)	479, 1012, 1174
Ksp22I	TGATCA	1 cut(s)	1036
KspI	CCGCGG	1 cut(s)	178
Kzo9I	GATC	5 cut(s)	253, 699, 755, 1006, 1036
LmnI	GCTCC	6 cut(s)	237, 295, 385, 490, 1081, 1417
Lsp1109I	GCAGC	7 cut(s)	168, 387, 421, 775, 1108, 1367, 1410
LweI	GCATC	3 cut(s)	649, 1023, 1399
MaeI	CTAG	5 cut(s)	770, 1226, 1472, 1631, 1685
MaeII	ACGT	1 cut(s)	727
MaeIII	GTNAC	8 cut(s)	642, 664, 692, 706, 723, 1187, 1241, 1604
MalI	GATC	5 cut(s)	255, 701, 757, 1008, 1038
MboI	GATC	5 cut(s)	253, 699, 755, 1006, 1036
MboII	GAAGA	6 cut(s)	887, 955, 1003, 1071, 1229, 1588
MflI	RGATCY	1 cut(s)	1006
MhlI	GDGCHC	4 cut(s)	242, 276, 495, 1225
MlsI	TGGCCA	1 cut(s)	545
MluCI	AATT	2 cut(s)	391, 1600
MluNI	TGGCCA	1 cut(s)	545
MlyI	GAGTC	3 cut(s)	1284, 1463, 1588
MnlI	CCTC	9 cut(s)	20, 154, 176, 327, 456, 636, 1329, 1513, 1703
Mox20I	TGGCCA	1 cut(s)	545
MroXI	GAANNNNTTC	1 cut(s)	368
MscI	TGGCCA	1 cut(s)	545
MseI	TTAA	8 cut(s)	825, 867, 918, 1131, 1308, 1506, 1734, 1741
MslI	CAYNNNNRTG	5 cut(s)	74, 104, 1019, 1086, 1440
Msp20I	TGGCCA	1 cut(s)	545
MspA1I	CMGCKG	3 cut(s)	177, 412, 1358
MspCI	CTTAAG	1 cut(s)	1733
MspI	CCGG	5 cut(s)	128, 218, 227, 314, 319
MspR9I	CCNGG	4 cut(s)	153, 219, 315, 320
Mva1269I	GAATGC	2 cut(s)	481, 845
MvaI	CCWGG	1 cut(s)	153
MvnI	CGCG	3 cut(s)	177, 522, 881
NciI	CCSGG	3 cut(s)	219, 315, 320
NcoI	CCATGG	2 cut(s)	75, 348
NdeII	GATC	5 cut(s)	253, 699, 755, 1006, 1036
NlaIV	GGNNCC	7 cut(s)	150, 297, 312, 342, 387, 565, 965
NmuCI	GTSAC	5 cut(s)	664, 692, 706, 723, 1604
NsbI	TGCGCA	2 cut(s)	480, 1013
NspI	RCATGY	2 cut(s)	217, 1445
OliI	CACNNNNGTG	1 cut(s)	104
PaeR7I	CTCGAG	1 cut(s)	241
PaqCI	CACCTGC	1 cut(s)	493
PcsI	WCGNNNNNNNCGW	1 cut(s)	299
PctI	GAATGC	2 cut(s)	481, 845
PdmI	GAANNNNTTC	1 cut(s)	368
PfeI	GAWTC	1 cut(s)	978
PflFI	GACNNNGTC	1 cut(s)	356
PflMI	CCANNNNNTGG	1 cut(s)	974
PleI	GAGTC	3 cut(s)	1284, 1462, 1587
PpsI	GAGTC	3 cut(s)	1284, 1462, 1587
Ppu21I	YACGTR	1 cut(s)	728
Psp124BI	GAGCTC	1 cut(s)	242
Psp6I	CCWGG	1 cut(s)	151
PspFI	CCCAGC	2 cut(s)	162, 1297
PspGI	CCWGG	1 cut(s)	151
PspN4I	GGNNCC	7 cut(s)	150, 297, 312, 342, 387, 565, 965
PspPI	GGNCC	5 cut(s)	148, 189, 316, 340, 815
PstI	CTGCAG	2 cut(s)	780, 1098
PsuI	RGATCY	1 cut(s)	1006
PsyI	GACNNNGTC	1 cut(s)	356
PvuII	CAGCTG	1 cut(s)	1358
RsaI	GTAC	5 cut(s)	425, 539, 1262, 1616, 1653
RsaNI	GTAC	5 cut(s)	424, 538, 1261, 1615, 1652
RseI	CAYNNNNRTG	5 cut(s)	74, 104, 1019, 1086, 1440
SacI	GAGCTC	1 cut(s)	242
SacII	CCGCGG	1 cut(s)	178
SaqAI	TTAA	8 cut(s)	825, 867, 918, 1131, 1308, 1506, 1734, 1741
Sau3AI	GATC	5 cut(s)	253, 699, 755, 1006, 1036
Sau96I	GGNCC	5 cut(s)	148, 189, 316, 340, 815
ScaI	AGTACT	1 cut(s)	539
SchI	GAGTC	3 cut(s)	1284, 1463, 1588
ScrFI	CCNGG	4 cut(s)	153, 219, 315, 320
SduI	GDGCHC	4 cut(s)	242, 276, 495, 1225
SfaNI	GCATC	3 cut(s)	649, 1023, 1399
SfcI	CTRYAG	4 cut(s)	776, 1071, 1094, 1655
Sfr274I	CTCGAG	1 cut(s)	241
Sfr303I	CCGCGG	1 cut(s)	178
SgrBI	CCGCGG	1 cut(s)	178
SinI	GGWCC	2 cut(s)	148, 340
SlaI	CTCGAG	1 cut(s)	241
SmiMI	CAYNNNNRTG	5 cut(s)	74, 104, 1019, 1086, 1440
SmlI	CTYRAG	4 cut(s)	241, 512, 1256, 1733
SmoI	CTYRAG	4 cut(s)	241, 512, 1256, 1733
Sse9I	AATT	2 cut(s)	391, 1600
SspI	AATATT	1 cut(s)	1135
SspMI	CTAG	5 cut(s)	770, 1226, 1472, 1631, 1685
SstI	GAGCTC	1 cut(s)	242
StyD4I	CCNGG	4 cut(s)	151, 217, 313, 318
StyI	CCWWGG	4 cut(s)	75, 279, 348, 1671
TaaI	ACNGT	7 cut(s)	118, 428, 550, 592, 670, 741, 1153
TaiI	ACGT	1 cut(s)	730
TaqI	TCGA	6 cut(s)	31, 242, 431, 758, 981, 1461
TaqII	GACCGA	1 cut(s)	215
TasI	AATT	2 cut(s)	391, 1600
TatI	WGTACW	4 cut(s)	423, 537, 1260, 1651
TauI	GCSGC	5 cut(s)	177, 190, 301, 421, 525
TfiI	GAWTC	1 cut(s)	978
Tru1I	TTAA	8 cut(s)	825, 867, 918, 1131, 1308, 1506, 1734, 1741
Tru9I	TTAA	8 cut(s)	825, 867, 918, 1131, 1308, 1506, 1734, 1741
TscAI	CASTG	2 cut(s)	121, 553
TseFI	GTSAC	5 cut(s)	664, 692, 706, 723, 1604
TseI	GCWGC	7 cut(s)	156, 400, 409, 788, 1096, 1355, 1423
Tsp45I	GTSAC	5 cut(s)	664, 692, 706, 723, 1604
TspDTI	ATGAA	4 cut(s)	740, 888, 916, 1479
TspGWI	ACGGA	3 cut(s)	398, 475, 1040
TspRI	CASTG	2 cut(s)	121, 553
Tth111I	GACNNNGTC	1 cut(s)	356
Van91I	CCANNNNNTGG	1 cut(s)	974
Vha464I	CTTAAG	1 cut(s)	1733
VpaK11BI	GGWCC	2 cut(s)	148, 340
XapI	RAATTY	1 cut(s)	391
XbaI	TCTAGA	1 cut(s)	1684
XceI	RCATGY	2 cut(s)	217, 1445
XhoI	CTCGAG	1 cut(s)	241
XmnI	GAANNNNTTC	1 cut(s)	368
XspI	CTAG	5 cut(s)	770, 1226, 1472, 1631, 1685
ZrmI	AGTACT	1 cut(s)	539

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.