Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Reverse (-)

2791193 .. 2794218

Gene Structure 3026 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1038400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGTATATCCAACACATCAGTCCGGCCTGTTCCACCACCGTCAACGACGCCCTTTCCCTCCCGCCCGTAACCCATCCCCTCAGCCCCGAGCTCATCAATATATCGGCTTCCGGACTGGCCGACCAAAACGGCGGTTGTGGGAACGATTTGTTGTCTGTCGTTTCGGAGCCGCCGATGTCAGTGCCGAGGTGGCAGAGGGTGGGGAGAGTTGTCCCGTCAATGGACGCGGTGGTGAAGGTGTTCTGCGTCCACACTGCGCCGAACTTCTCGCTGCCGTGGCAGAGGAAGAAGCAGTACAGCTCCAGCAGTAGTGGGTTTGTGATTGGGGGCAGGATGATTTTGACCAATGCGCATTCGGTGGATCATCATACCCAGGTCAAGCTCAAGAAACGGGGCTCCGACACCAAGTATTTGGCCACTGTGTTGGCCGTTGGAGCGGAATGCGATATCGCGATGCTTACGGTGAGTGATGATGAGTTCTGGGAAGGGATTTCACCTGTGGAGTTTGGAGAGTTGCCTGCACTGCAAGATGCCGTGACAGTGGTTGGGTACCCGATTGGGGGTGACACAATCTCCGTGACGAGCGGAGTTGTGTCGAGGATGGAGATCCTGCCTTATGTTCATGGCTTCACCGAGCTTCTGGGAATGCAGATAGATGCTGCAATCAACTCTGGAAACTCTGGTGGCCCTGCCTTTAATGATAAGGGAGAATGCGTGGGAATTGCATTTCAGTGCCTCAAACATGAAGATGCGGAAAATATAGGCTATGTTATACCAGTACCAGTTATTATGCATTTCATTCGAGATTATGAGAAAAATGGGGCATATACAGGGTTCCCAATTCTCGGAATTGAGTGGCAAAAGATGGAGAATCCTGATCTTCGCATGTCAATGGGGATGAGACCTGATCAAAAGGGTATCCGTATTAGAAGGCTGGAACCTACAGCTCCAGAATCTCAACTGCTAAAGCCATCTGATGTTATTCTTAGTTTTGATGGGGTTAACATTGCTAGTGATGGCACAGTTCCATTCAGGAATGGAGAGCGCATAGGTTTCAGTTACCTTGTCACTCAAAAATACATACGTGACAATGCTCTAATAAAAGTTCTTCGGAACTTTGAGACACTTGAGTTCAACATTAAACTTGCAAAACACAAGGAACTCATCCCATCACACATCGAGGGAAAACCTCCTTCCTTTTATATCATTGCTGGATTCGTTTTCACAGCTGTATCTGTTCCATATCTGCGTTCTGAGTTTGGAAATATATTTGACGTTCCAATCAAACTGTTAGACAAGCATTTACATGCAATGGCACAGTCTATTGACGAACAGCTCATTGTTGTTTCTCAGGTACTTGTTGCTGACATTAATATTGGATATGAGGACATCGTTAACAATCAGGTTCTTACTTTCAACGGTATGCCTGTGAAGAATCTGAAGAACTTGGCCAGCATGATTGAGAATTGTGATGATGAGTACCTTAAGTTCGGCCTTGAATACAATCAGATGGTTGTTCTGCAAACAAAAACTGCCAAGGCAGCAACGCTGGATATCCTCACGACACATTGCATATCATCGTCCATGTCCGATGATCTTAAGACTAAAGAGAATACTTTAGAGGAAGTACATTCTACGGCCGATGACCTTAGAGATACGATCTTAGAGAAAGTAGAGGCTGTCTACTTGCCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

565

Amino Acids

62.1

Weight (kDa)

5.26

Isoelectric Point (pI)

45.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	84 - 267	3.2e-19	Trypsin
Trypsin_2	PF13365	104 - 241	1.1e-20	Trypsin-like peptidase domain
PDZ_3	PF17815	389 - 532	5e-43	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	351
Acc65I	GGTACC	1 cut(s)	549
AccB1I	GGYRCC	1 cut(s)	549
AccBSI	CCGCTC	2 cut(s)	437, 585
AccI	GTMKAC	1 cut(s)	1683
AccII	CGCG	2 cut(s)	227, 452
AccIII	TCCGGA	1 cut(s)	110
AciI	CCGC	7 cut(s)	62, 132, 170, 227, 437, 585, 752
AclWI	GGATC	2 cut(s)	369, 601
AcoI	YGGCCR	5 cut(s)	117, 414, 426, 1449, 1638
AcuI	CTGAAG	1 cut(s)	1460
AcyI	GRCGYC	1 cut(s)	48
AfaI	GTAC	6 cut(s)	296, 551, 780, 1356, 1481, 1629
AfiI	CCNNNNNNNGG	4 cut(s)	220, 559, 560, 845
AflII	CTTAAG	2 cut(s)	1484, 1598
AgsI	TTSAA	3 cut(s)	1135, 1417, 1499
AjnI	CCWGG	1 cut(s)	372
AluBI	AGCT	7 cut(s)	91, 300, 382, 637, 947, 1229, 1336
AluI	AGCT	7 cut(s)	91, 300, 382, 637, 947, 1229, 1336
Alw21I	GWGCWC	1 cut(s)	93
Alw26I	GTCTC	2 cut(s)	895, 1115
AlwI	GGATC	2 cut(s)	369, 601
Ama87I	CYCGRG	1 cut(s)	86
Aor13HI	TCCGGA	1 cut(s)	110
AoxI	GGCC	8 cut(s)	24, 117, 414, 426, 685, 1449, 1492, 1638
ApeKI	GCWGC	3 cut(s)	271, 659, 1541
AseI	ATTAAT	1 cut(s)	1371
Asp700I	GAANNNNTTC	1 cut(s)	239
Asp718I	GGTACC	1 cut(s)	549
AspLEI	GCGC	3 cut(s)	259, 352, 1047
AspS9I	GGNCC	1 cut(s)	686
AsuHPI	GGTGA	5 cut(s)	244, 475, 486, 575, 622
AvaI	CYCGRG	1 cut(s)	86
BaeI	ACNNNNGTAYC	2 cut(s)	1215, 1248
BalI	TGGCCA	2 cut(s)	416, 1451
BanI	GGYRCC	1 cut(s)	549
BanII	GRGCYC	2 cut(s)	93, 398
BarI	GAAGNNNNNNTAC	2 cut(s)	278, 310
Bbv12I	GWGCWC	1 cut(s)	93
BbvCI	CCTCAGC	1 cut(s)	80
BbvI	GCAGC	3 cut(s)	258, 646, 1553
BccI	CCATC	8 cut(s)	81, 595, 859, 979, 989, 1010, 1177, 1504
BceAI	ACGGC	5 cut(s)	145, 259, 413, 518, 1653
BcgI	CGANNNNNNTGC	4 cut(s)	163, 197, 782, 816
BciT130I	CCWGG	1 cut(s)	374
BciVI	GTATCC	1 cut(s)	929
BclI	TGATCA	1 cut(s)	907
BcoDI	GTCTC	2 cut(s)	895, 1115
BfaI	CTAG	1 cut(s)	1011
BfmI	CTRYAG	1 cut(s)	942
BfrI	CTTAAG	2 cut(s)	1484, 1598
BfuI	GTATCC	1 cut(s)	929
BglI	GCCNNNNNGGC	1 cut(s)	190
BisI	GCNGC	4 cut(s)	170, 272, 660, 1542
BlsI	GCNGC	4 cut(s)	171, 273, 661, 1543
Bme1390I	CCNGG	1 cut(s)	374
BmeT110I	CYCGRG	1 cut(s)	86
BmgT120I	GGNCC	1 cut(s)	686
BmiI	GGNNCC	5 cut(s)	168, 397, 551, 836, 939
BmrFI	CCNGG	1 cut(s)	374
BmsI	GCATC	4 cut(s)	444, 520, 646, 739
BpmI	CTGGAG	2 cut(s)	286, 933
Bpu10I	CCTNAGC	1 cut(s)	80
BpuEI	CTTGAG	2 cut(s)	368, 1148
BsaAI	YACGTR	1 cut(s)	1085
BsaBI	GATNNNNATC	1 cut(s)	605
BsaHI	GRCGYC	1 cut(s)	48
BsaI	GGTCTC	1 cut(s)	895
BsaJI	CCNNGG	4 cut(s)	185, 275, 372, 1536
BsaWI	WCCGGW	1 cut(s)	110
BsaXI	ACNNNNNCTCC	4 cut(s)	557, 587, 699, 729
Bsc4I	CCNNNNNNNGG	4 cut(s)	220, 559, 560, 845
Bse1I	ACTGG	3 cut(s)	120, 776, 782
Bse3DI	GCAATG	4 cut(s)	1005, 1206, 1317, 1567
Bse8I	GATNNNNATC	1 cut(s)	605
BseAI	TCCGGA	1 cut(s)	110
BseBI	CCWGG	1 cut(s)	374
BseDI	CCNNGG	4 cut(s)	185, 275, 372, 1536
BseGI	GGATG	5 cut(s)	73, 339, 606, 903, 1164
BseJI	GATNNNNATC	1 cut(s)	605
BseLI	CCNNNNNNNGG	4 cut(s)	220, 559, 560, 845
BseMI	GCAATG	4 cut(s)	1005, 1206, 1317, 1567
BseMII	CTCAG	3 cut(s)	94, 1245, 1364
BseNI	ACTGG	3 cut(s)	120, 776, 782
BseX3I	CGGCCG	1 cut(s)	1638
BseXI	GCAGC	3 cut(s)	258, 646, 1553
BsgI	GTGCAG	1 cut(s)	504
Bsh1236I	CGCG	2 cut(s)	227, 452
Bsh1285I	CGRYCG	1 cut(s)	1641
BshFI	GGCC	8 cut(s)	26, 119, 416, 428, 687, 1451, 1494, 1640
BshNI	GGYRCC	1 cut(s)	549
BsiEI	CGRYCG	1 cut(s)	1641
BsiHKAI	GWGCWC	1 cut(s)	93
BsiHKCI	CYCGRG	1 cut(s)	86
BsiSI	CCGG	2 cut(s)	23, 111
BslFI	GGGAC	1 cut(s)	197
BslI	CCNNNNNNNGG	4 cut(s)	220, 559, 560, 845
BsmAI	GTCTC	2 cut(s)	895, 1115
BsmFI	GGGAC	1 cut(s)	197
BsmI	GAATGC	4 cut(s)	352, 446, 651, 716
BsnI	GGCC	8 cut(s)	26, 119, 416, 428, 687, 1451, 1494, 1640
Bso31I	GGTCTC	1 cut(s)	895
BsoBI	CYCGRG	1 cut(s)	86
Bsp1286I	GDGCHC	2 cut(s)	93, 398
Bsp13I	TCCGGA	1 cut(s)	110
Bsp143I	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
Bsp68I	TCGCGA	1 cut(s)	452
BspACI	CCGC	7 cut(s)	62, 132, 170, 227, 437, 585, 752
BspANI	GGCC	8 cut(s)	26, 119, 416, 428, 687, 1451, 1494, 1640
BspCNI	CTCAG	3 cut(s)	93, 1246, 1363
BspEI	TCCGGA	1 cut(s)	110
BspFNI	CGCG	2 cut(s)	227, 452
BspLI	GGNNCC	5 cut(s)	168, 397, 551, 836, 939
BspPI	GGATC	2 cut(s)	369, 601
BspT107I	GGYRCC	1 cut(s)	549
BspTI	CTTAAG	2 cut(s)	1484, 1598
BspTNI	GGTCTC	1 cut(s)	895
BsrBI	CCGCTC	2 cut(s)	437, 585
BsrDI	GCAATG	4 cut(s)	1005, 1206, 1317, 1567
BsrI	ACTGG	3 cut(s)	120, 776, 782
BssECI	CCNNGG	4 cut(s)	185, 275, 372, 1536
BssMI	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
BssNI	GRCGYC	1 cut(s)	48
BssT1I	CCWWGG	1 cut(s)	1536
Bst2UI	CCWGG	1 cut(s)	374
Bst4CI	ACNGT	8 cut(s)	40, 421, 463, 541, 1024, 1290, 1320, 1421
BstACI	GRCGYC	1 cut(s)	48
BstAFI	CTTAAG	2 cut(s)	1484, 1598
BstBAI	YACGTR	1 cut(s)	1085
BstC8I	GCNNGC	2 cut(s)	519, 1453
BstDEI	CTNAG	6 cut(s)	80, 986, 1254, 1350, 1649, 1663
BstDSI	CCRYGG	1 cut(s)	275
BstF5I	GGATG	5 cut(s)	73, 339, 606, 903, 1164
BstFNI	CGCG	2 cut(s)	227, 452
BstHHI	GCGC	3 cut(s)	259, 352, 1047
BstKTI	GATC	6 cut(s)	364, 609, 880, 910, 1597, 1662
BstMAI	GTCTC	2 cut(s)	895, 1115
BstMBI	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
BstMCI	CGRYCG	1 cut(s)	1641
BstMWI	GCNNNNNNNGC	5 cut(s)	190, 277, 434, 523, 1541
BstNI	CCWGG	1 cut(s)	374
BstNSI	RCATGY	2 cut(s)	889, 1310
BstSCI	CCNGG	1 cut(s)	372
BstSFI	CTRYAG	1 cut(s)	942
BstUI	CGCG	2 cut(s)	227, 452
BstV1I	GCAGC	3 cut(s)	258, 646, 1553
BstX2I	RGATCY	1 cut(s)	606
BstXI	CCANNNNNNTGG	2 cut(s)	412, 424
BstYI	RGATCY	1 cut(s)	606
BstZI	CGGCCG	1 cut(s)	1638
BsuI	GTATCC	1 cut(s)	929
BsuRI	GGCC	8 cut(s)	26, 119, 416, 428, 687, 1451, 1494, 1640
BtgI	CCRYGG	1 cut(s)	275
BtgZI	GCGATG	1 cut(s)	467
BtsCI	GGATG	5 cut(s)	73, 339, 606, 903, 1164
BtsI	GCAGTG	2 cut(s)	252, 521
BtsIMutI	CAGTG	6 cut(s)	186, 252, 417, 521, 546, 737
BtuMI	TCGCGA	1 cut(s)	452
Cac8I	GCNNGC	2 cut(s)	519, 1453
CfoI	GCGC	3 cut(s)	259, 352, 1047
Cfr13I	GGNCC	1 cut(s)	686
CseI	GACGC	3 cut(s)	56, 233, 235
Csp6I	GTAC	6 cut(s)	295, 550, 779, 1355, 1480, 1628
CviAII	CATG	6 cut(s)	623, 743, 886, 1307, 1456, 1585
CviQI	GTAC	6 cut(s)	295, 550, 779, 1355, 1480, 1628
DdeI	CTNAG	6 cut(s)	80, 986, 1254, 1350, 1649, 1663
DpnI	GATC	6 cut(s)	363, 608, 879, 909, 1596, 1661
DpnII	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
EaeI	YGGCCR	5 cut(s)	117, 414, 426, 1449, 1638
EagI	CGGCCG	1 cut(s)	1638
Ecl136II	GAGCTC	1 cut(s)	91
EclXI	CGGCCG	1 cut(s)	1638
Eco130I	CCWWGG	1 cut(s)	1536
Eco24I	GRGCYC	2 cut(s)	93, 398
Eco31I	GGTCTC	1 cut(s)	895
Eco32I	GATATC	2 cut(s)	448, 1555
Eco52I	CGGCCG	1 cut(s)	1638
Eco53kI	GAGCTC	1 cut(s)	91
Eco57I	CTGAAG	1 cut(s)	1460
Eco88I	CYCGRG	1 cut(s)	86
EcoICRI	GAGCTC	1 cut(s)	91
EcoRII	CCWGG	1 cut(s)	372
EcoRV	GATATC	2 cut(s)	448, 1555
EcoT14I	CCWWGG	1 cut(s)	1536
EcoT22I	ATGCAT	1 cut(s)	795
EcoT38I	GRGCYC	2 cut(s)	93, 398
ErhI	CCWWGG	1 cut(s)	1536
FaeI	CATG	6 cut(s)	626, 746, 889, 1310, 1459, 1588
FaqI	GGGAC	1 cut(s)	197
FatI	CATG	6 cut(s)	622, 742, 885, 1306, 1455, 1584
FauI	CCCGC	1 cut(s)	69
FbaI	TGATCA	1 cut(s)	907
FblI	GTMKAC	1 cut(s)	1683
Fnu4HI	GCNGC	4 cut(s)	170, 272, 660, 1542
FokI	GGATG	5 cut(s)	60, 346, 613, 910, 1151
FriOI	GRGCYC	2 cut(s)	93, 398
Fsp4HI	GCNGC	4 cut(s)	170, 272, 660, 1542
FspAI	RTGCGCAY	1 cut(s)	351
FspBI	CTAG	1 cut(s)	1011
FspI	TGCGCA	1 cut(s)	351
GlaI	GCGC	3 cut(s)	258, 351, 1046
GluI	GCNGC	4 cut(s)	170, 272, 660, 1542
GsuI	CTGGAG	2 cut(s)	286, 933
HaeIII	GGCC	8 cut(s)	26, 119, 416, 428, 687, 1451, 1494, 1640
HapII	CCGG	2 cut(s)	23, 111
HgaI	GACGC	3 cut(s)	56, 233, 235
HhaI	GCGC	3 cut(s)	259, 352, 1047
Hin1I	GRCGYC	1 cut(s)	48
Hin1II	CATG	6 cut(s)	626, 746, 889, 1310, 1459, 1588
Hin6I	GCGC	3 cut(s)	257, 350, 1045
HinP1I	GCGC	3 cut(s)	257, 350, 1045
HincII	GTYRAC	3 cut(s)	43, 1003, 1396
HindII	GTYRAC	3 cut(s)	43, 1003, 1396
HinfI	GANTC	4 cut(s)	871, 953, 1215, 1435
HpaI	GTTAAC	2 cut(s)	1003, 1396
HpaII	CCGG	2 cut(s)	23, 111
HphI	GGTGA	5 cut(s)	244, 475, 486, 575, 622
Hpy166II	GTNNAC	5 cut(s)	43, 250, 1003, 1396, 1684
Hpy188I	TCNGA	9 cut(s)	166, 400, 848, 976, 1113, 1255, 1440, 1509, 1591
Hpy188III	TCNNGA	9 cut(s)	111, 385, 451, 672, 803, 875, 950, 1033, 1561
Hpy8I	GTNNAC	5 cut(s)	43, 250, 1003, 1396, 1684
Hpy99I	CGWCG	1 cut(s)	50
HpyAV	CCTTC	4 cut(s)	229, 479, 924, 1203
HpyCH4III	ACNGT	8 cut(s)	40, 421, 463, 541, 1024, 1290, 1320, 1421
HpyCH4IV	ACGT	2 cut(s)	1084, 1275
HpyF10VI	GCNNNNNNNGC	5 cut(s)	190, 277, 434, 523, 1541
HpyF3I	CTNAG	6 cut(s)	80, 986, 1254, 1350, 1649, 1663
HpySE526I	ACGT	2 cut(s)	1084, 1275
Hsp92I	GRCGYC	1 cut(s)	48
Hsp92II	CATG	6 cut(s)	626, 746, 889, 1310, 1459, 1588
HspAI	GCGC	3 cut(s)	257, 350, 1045
Kpn2I	TCCGGA	1 cut(s)	110
KpnI	GGTACC	1 cut(s)	553
Ksp22I	TGATCA	1 cut(s)	907
KspAI	GTTAAC	2 cut(s)	1003, 1396
Kzo9I	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
LmnI	GCTCC	5 cut(s)	166, 305, 401, 434, 952
Lsp1109I	GCAGC	3 cut(s)	258, 646, 1553
LweI	GCATC	4 cut(s)	444, 520, 646, 739
MaeI	CTAG	1 cut(s)	1011
MaeII	ACGT	2 cut(s)	1084, 1275
MaeIII	GTNAC	7 cut(s)	67, 535, 563, 577, 1058, 1066, 1085
MalI	GATC	6 cut(s)	363, 608, 879, 909, 1596, 1661
MbiI	CCGCTC	2 cut(s)	437, 585
MboI	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
MboII	GAAGA	6 cut(s)	298, 758, 872, 1100, 1444, 1453
MflI	RGATCY	1 cut(s)	606
MhlI	GDGCHC	2 cut(s)	93, 398
MlsI	TGGCCA	2 cut(s)	416, 1451
MluCI	AATT	4 cut(s)	720, 840, 849, 1465
MluNI	TGGCCA	2 cut(s)	416, 1451
MmeI	TCCRAC	3 cut(s)	34, 412, 423
Mox20I	TGGCCA	2 cut(s)	416, 1451
Mph1103I	ATGCAT	1 cut(s)	795
MroI	TCCGGA	1 cut(s)	110
MroXI	GAANNNNTTC	1 cut(s)	239
MscI	TGGCCA	2 cut(s)	416, 1451
MseI	TTAA	8 cut(s)	696, 1002, 1140, 1371, 1395, 1485, 1599, 1693
MslI	CAYNNNNRTG	4 cut(s)	730, 747, 890, 1305
Msp20I	TGGCCA	2 cut(s)	416, 1451
MspA1I	CMGCKG	1 cut(s)	1229
MspCI	CTTAAG	2 cut(s)	1484, 1598
MspI	CCGG	2 cut(s)	23, 111
MspR9I	CCNGG	1 cut(s)	374
Mva1269I	GAATGC	4 cut(s)	352, 446, 651, 716
MvaI	CCWGG	1 cut(s)	374
MvnI	CGCG	2 cut(s)	227, 452
MwoI	GCNNNNNNNGC	5 cut(s)	190, 277, 434, 523, 1541
NdeII	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
NlaIII	CATG	6 cut(s)	626, 746, 889, 1310, 1459, 1588
NlaIV	GGNNCC	5 cut(s)	168, 397, 551, 836, 939
NmeAIII	GCCGAG	1 cut(s)	210
NmuCI	GTSAC	5 cut(s)	535, 563, 577, 1066, 1085
NruI	TCGCGA	1 cut(s)	452
NsbI	TGCGCA	1 cut(s)	351
NsiI	ATGCAT	1 cut(s)	795
NspI	RCATGY	2 cut(s)	889, 1310
PcsI	WCGNNNNNNNCGW	1 cut(s)	170
PctI	GAATGC	4 cut(s)	352, 446, 651, 716
PdmI	GAANNNNTTC	1 cut(s)	239
PfeI	GAWTC	4 cut(s)	871, 953, 1215, 1435
PkrI	GCNGC	4 cut(s)	171, 273, 661, 1543
Ppu21I	YACGTR	1 cut(s)	1085
PshBI	ATTAAT	1 cut(s)	1371
Psp124BI	GAGCTC	1 cut(s)	93
Psp6I	CCWGG	1 cut(s)	372
PspGI	CCWGG	1 cut(s)	372
PspN4I	GGNNCC	5 cut(s)	168, 397, 551, 836, 939
PspPI	GGNCC	1 cut(s)	686
PsrI	GAACNNNNNNTAC	2 cut(s)	1472, 1504
PsuI	RGATCY	1 cut(s)	606
PvuII	CAGCTG	1 cut(s)	1229
RruI	TCGCGA	1 cut(s)	452
RsaI	GTAC	6 cut(s)	296, 551, 780, 1356, 1481, 1629
RsaNI	GTAC	6 cut(s)	295, 550, 779, 1355, 1480, 1628
RseI	CAYNNNNRTG	4 cut(s)	730, 747, 890, 1305
SacI	GAGCTC	1 cut(s)	93
SaqAI	TTAA	8 cut(s)	696, 1002, 1140, 1371, 1395, 1485, 1599, 1693
SatI	GCNGC	4 cut(s)	170, 272, 660, 1542
Sau3AI	GATC	6 cut(s)	361, 606, 877, 907, 1594, 1659
Sau96I	GGNCC	1 cut(s)	686
ScrFI	CCNGG	1 cut(s)	374
SduI	GDGCHC	2 cut(s)	93, 398
SfaNI	GCATC	4 cut(s)	444, 520, 646, 739
SfcI	CTRYAG	1 cut(s)	942
SmiMI	CAYNNNNRTG	4 cut(s)	730, 747, 890, 1305
SmlI	CTYRAG	4 cut(s)	383, 1127, 1484, 1598
SmoI	CTYRAG	4 cut(s)	383, 1127, 1484, 1598
Sse9I	AATT	4 cut(s)	720, 840, 849, 1465
SsiI	CCGC	7 cut(s)	62, 132, 170, 227, 437, 585, 752
SspI	AATATT	1 cut(s)	1375
SspMI	CTAG	1 cut(s)	1011
SstI	GAGCTC	1 cut(s)	93
StyD4I	CCNGG	1 cut(s)	372
StyI	CCWWGG	1 cut(s)	1536
TaaI	ACNGT	8 cut(s)	40, 421, 463, 541, 1024, 1290, 1320, 1421
TaiI	ACGT	2 cut(s)	1087, 1278
TaqI	TCGA	3 cut(s)	596, 802, 1179
TasI	AATT	4 cut(s)	720, 840, 849, 1465
TatI	WGTACW	2 cut(s)	294, 1627
TauI	GCSGC	1 cut(s)	172
TfiI	GAWTC	4 cut(s)	871, 953, 1215, 1435
Tru1I	TTAA	8 cut(s)	696, 1002, 1140, 1371, 1395, 1485, 1599, 1693
Tru9I	TTAA	8 cut(s)	696, 1002, 1140, 1371, 1395, 1485, 1599, 1693
TscAI	CASTG	6 cut(s)	186, 259, 424, 528, 546, 737
TseFI	GTSAC	5 cut(s)	535, 563, 577, 1066, 1085
TseI	GCWGC	3 cut(s)	271, 659, 1541
Tsp45I	GTSAC	5 cut(s)	535, 563, 577, 1066, 1085
TspDTI	ATGAA	3 cut(s)	611, 759, 787
TspGWI	ACGGA	2 cut(s)	565, 911
TspRI	CASTG	6 cut(s)	186, 259, 424, 528, 546, 737
Vha464I	CTTAAG	2 cut(s)	1484, 1598
VspI	ATTAAT	1 cut(s)	1371
XceI	RCATGY	2 cut(s)	889, 1310
XmiI	GTMKAC	1 cut(s)	1683
XmnI	GAANNNNTTC	1 cut(s)	239
XspI	CTAG	1 cut(s)	1011
Zsp2I	ATGCAT	1 cut(s)	795

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.