Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Reverse (-)

27700883 .. 27704737

Gene Structure 3855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1184600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGGCAAACGAGGCCGCAAGCCCAAAACCCCCACCCCCGAAACCCTAGATTTACCCGCCACCGCCATGGCATCCCCACCGGCCCCGCCGGTTACGACGGCCACAACCGCAGTTGACGACGTTTTCTCCGTCAGCAACGTGGAGATAATCGAGCAGCCTCAGGCCTCGCAGCCCCAACACGAAGGTCGCCGCCGCGGTCGACCTAAGAAGCTCCAAAAGCTCTCCGACAAGCCCGAGGCCATGGCCCCTTCCCGGAGGATCCTTCGGGCGGTCGACAATGGTGACCACAAGGGGGCGGTGGGGCCGACCTTGACGGTTTCGGATCCTGGTGAGGCGTGGGAGGGCGGGGCGAGGGTGGTGCCGGCCATGGATGCGGTGGTGAAGGTGTTTTGTACGCATACAGAGCCGAATTTCTCGCTGCCGTGGCAGAGGAAGAGGCAGTACAGCTCGAGCAGCAGTGGGTTTGTGATTGGTGGGAGGAGGGTGCTGACCAATGCGCACTCTGTGGAGCATTATACGCAGGTCAAGCTCAAGAAGCGTGGCTCCGATACTAAGTACTTGGCCACTGTTCTCGCCATTGGCACCGAGTGCGATATTGCCATGCTTACTGTTGATGACAACGAGTTTTGGAAAGGAGTGTCACCAGTGGAGTTTGGGGATTTGCCTGCGCTTCAAGACGCCGTTACTGTTGTGGGTTACCCAATTGGAGGAGACACAATTTCAGTGACGAGTGGTGTTGTGTCACGAATAGAGATTCTGTCATATGTTCATGGGTCTACTGAACTACTGGGTTTGCAGATAGATGCTGCCATTAACTCTGGAAACTCAGGTGGGCCTGCTTTTAATGACAAAGGAAATTGCGTCGGTATCGCATTTCAGTCCCTTAAACATGAAGATGCAGAGAATATTGGTTATGTCATACCAACACCAGTCATTATGCACTTCATCCAAGATTATGAGAGGAATGGAGCATATACAGGATTTCCAATTCTTGGGGTTGAGTGGCAGAAGATGGAAAATCCTGATTTGCGTATGGCAATGGGCATGAAACCTGATCAGAAGGGTGTTCGTATAAGAAGAGTTGATCCCACTGCTCCGGAATCTCATGTTCTGAAGCCATCTGATATCATTCTCAGCTTTGATGGAGTTAATATTGCCAATGATGGAACAGTTCCTTTTCGGCATGGAGAACGCATAGGTTTTAGTTACCTTGTCTCCCAGAAATATACTGGAGATAATTCAGCGGTTAAAGTTCTGCGCAATTCTGATATTCTCAATTTTGACATCAAACTTGCATCCCACAAAAGGCTAATCCCAGCACACAACAAGGGCAGACCTCCTTCATATTATATAATTGCAGGATTTGTCTTTACAGCTGTGTCTGTTCCTTATCTTCGATCTGAGTATGGAAAGGAATATGAATTTGAAGCTCCTGTCAAGCTTTTGGACAAGATGCTGCATTCAATGCCACAATCACCAGATGAGCAGCTCGTCGTTGTTTCACAGGTCCTTGTGGCTGACATCAACATCGGTTATGAAGAAATAGTTAACACCCAGGTTCTTGCTTTAAATGGTAAGCCTGTGAAAAACCTGAAGAACTTGGCAAGCATGGTAGAGAGCTGCGAGGATGAATTTTTGAAATTTGATTTAGAATACCAACAGGTGGTGGTTCTCCGAACAAAAACTGCTAAAGCAGCTACTCTAGATATTCTTGCTACACATTGCATACCCTCAGCAATGTCTGATGATCTGAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

587

Amino Acids

64.06

Weight (kDa)

6.42

Isoelectric Point (pI)

49.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	135 - 315	5.4e-19	Trypsin
Trypsin_2	PF13365	153 - 290	6.1e-21	Trypsin-like peptidase domain
PDZ_2	PF13180	340 - 431	6.8e-06	PDZ domain
PDZ_3	PF17815	438 - 583	1.1e-49	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	498, 1259
Acc36I	ACCTGC	1 cut(s)	511
AccB1I	GGYRCC	2 cut(s)	358, 581
AccB7I	CCANNNNNTGG	2 cut(s)	992, 1663
AccI	GTMKAC	3 cut(s)	199, 273, 776
AccII	CGCG	1 cut(s)	195
AccIII	TCCGGA	1 cut(s)	1096
AclWI	GGATC	5 cut(s)	253, 266, 317, 330, 1079
AcoI	YGGCCR	3 cut(s)	99, 363, 561
AcsI	RAATTY	4 cut(s)	409, 1421, 1631, 1640
AcuI	CTGAAG	2 cut(s)	1133, 1613
AcyI	GRCGYC	1 cut(s)	678
AdeI	CACNNNGTG	2 cut(s)	505, 588
AfaI	GTAC	3 cut(s)	394, 443, 557
AfiI	CCNNNNNNNGG	7 cut(s)	252, 268, 292, 707, 992, 1305, 1663
AgsI	TTSAA	4 cut(s)	674, 1427, 1464, 1639
AjnI	CCWGG	2 cut(s)	325, 1554
Alw26I	GTCTC	2 cut(s)	705, 1219
AlwI	GGATC	5 cut(s)	253, 266, 317, 330, 1079
Ama87I	CYCGRG	2 cut(s)	233, 448
Aor13HI	TCCGGA	1 cut(s)	1096
ApeKI	GCWGC	9 cut(s)	154, 169, 418, 453, 806, 1456, 1486, 1620, 1694
ApoI	RAATTY	4 cut(s)	409, 1421, 1631, 1640
AspLEI	GCGC	3 cut(s)	499, 670, 1260
AspS9I	GGNCC	5 cut(s)	82, 244, 302, 833, 1507
AsuC2I	CCSGG	1 cut(s)	253
AsuHPI	GGTGA	5 cut(s)	293, 341, 391, 633, 1467
AvaI	CYCGRG	2 cut(s)	233, 448
AvaII	GGWCC	1 cut(s)	1507
AxyI	CCTNAGG	1 cut(s)	159
BalI	TGGCCA	1 cut(s)	563
BamHI	GGATCC	2 cut(s)	258, 322
BanI	GGYRCC	2 cut(s)	358, 581
BarI	GAAGNNNNNNTAC	2 cut(s)	425, 457
BbvCI	CCTCAGC	1 cut(s)	1732
BbvI	GCAGC	9 cut(s)	166, 181, 405, 465, 793, 1443, 1498, 1607, 1706
BccI	CCATC	4 cut(s)	1006, 1126, 1136, 1157
BceAI	ACGGC	3 cut(s)	114, 406, 665
BcgI	CGANNNNNNTGC	2 cut(s)	340, 374
BciT130I	CCWGG	2 cut(s)	327, 1556
BclI	TGATCA	1 cut(s)	1054
BcnI	CCSGG	1 cut(s)	253
BcoDI	GTCTC	2 cut(s)	705, 1219
BfaI	CTAG	2 cut(s)	47, 1703
BfuAI	ACCTGC	1 cut(s)	511
BmcAI	AGTACT	1 cut(s)	557
Bme1390I	CCNGG	3 cut(s)	253, 327, 1556
Bme18I	GGWCC	1 cut(s)	1507
BmeT110I	CYCGRG	2 cut(s)	233, 448
BmgT120I	GGNCC	5 cut(s)	82, 244, 302, 833, 1507
BmiI	GGNNCC	8 cut(s)	84, 246, 260, 303, 324, 360, 544, 583
BmrFI	CCNGG	3 cut(s)	253, 327, 1556
BmrI	ACTGGG	1 cut(s)	797
BmsI	GCATC	6 cut(s)	80, 361, 793, 886, 1304, 1443
BmuI	ACTGGG	1 cut(s)	797
BpmI	CTGGAG	1 cut(s)	1251
Bpu10I	CCTNAGC	1 cut(s)	1732
BpuEI	CTTGAG	1 cut(s)	515
BpuMI	CCSGG	1 cut(s)	253
BsaHI	GRCGYC	1 cut(s)	678
BsaJI	CCNNGG	7 cut(s)	66, 193, 234, 240, 366, 422, 1554
BsaWI	WCCGGW	1 cut(s)	1096
BsaXI	ACNNNNNCTCC	4 cut(s)	110, 140, 1199, 1229
Bsc4I	CCNNNNNNNGG	7 cut(s)	252, 268, 292, 707, 992, 1305, 1663
Bse118I	RCCGGY	3 cut(s)	79, 88, 361
Bse1I	ACTGG	4 cut(s)	644, 792, 929, 1234
Bse21I	CCTNAGG	1 cut(s)	159
Bse3DI	GCAATG	3 cut(s)	1044, 1720, 1743
BseAI	TCCGGA	1 cut(s)	1096
BseBI	CCWGG	2 cut(s)	327, 1556
BseDI	CCNNGG	7 cut(s)	66, 193, 234, 240, 366, 422, 1554
BseGI	GGATG	5 cut(s)	71, 376, 945, 1295, 1633
BseLI	CCNNNNNNNGG	7 cut(s)	252, 268, 292, 707, 992, 1305, 1663
BseMI	GCAATG	3 cut(s)	1044, 1720, 1743
BseMII	CTCAG	5 cut(s)	173, 840, 1147, 1392, 1746
BseNI	ACTGG	4 cut(s)	644, 792, 929, 1234
BseRI	GAGGAG	2 cut(s)	493, 723
BseXI	GCAGC	9 cut(s)	166, 181, 405, 465, 793, 1443, 1498, 1607, 1706
BseYI	CCCAGC	1 cut(s)	1315
Bsh1236I	CGCG	1 cut(s)	195
Bsh1285I	CGRYCG	2 cut(s)	199, 273
BshNI	GGYRCC	2 cut(s)	358, 581
BsiEI	CGRYCG	2 cut(s)	199, 273
BsiHKCI	CYCGRG	2 cut(s)	233, 448
BsiSI	CCGG	5 cut(s)	80, 89, 253, 362, 1097
BslFI	GGGAC	1 cut(s)	865
BslI	CCNNNNNNNGG	7 cut(s)	252, 268, 292, 707, 992, 1305, 1663
BsmAI	GTCTC	2 cut(s)	705, 1219
BsmFI	GGGAC	1 cut(s)	865
BsmI	GAATGC	1 cut(s)	1459
BsoBI	CYCGRG	2 cut(s)	233, 448
Bsp13I	TCCGGA	1 cut(s)	1096
Bsp143I	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
Bsp19I	CCATGG	3 cut(s)	66, 240, 366
BspCNI	CTCAG	5 cut(s)	172, 839, 1146, 1393, 1745
BspEI	TCCGGA	1 cut(s)	1096
BspFNI	CGCG	1 cut(s)	195
BspLI	GGNNCC	8 cut(s)	84, 246, 260, 303, 324, 360, 544, 583
BspMI	ACCTGC	1 cut(s)	511
BspPI	GGATC	5 cut(s)	253, 266, 317, 330, 1079
BspT107I	GGYRCC	2 cut(s)	358, 581
BsrDI	GCAATG	3 cut(s)	1044, 1720, 1743
BsrFI	RCCGGY	3 cut(s)	79, 88, 361
BsrI	ACTGG	4 cut(s)	644, 792, 929, 1234
BssAI	RCCGGY	3 cut(s)	79, 88, 361
BssECI	CCNNGG	7 cut(s)	66, 193, 234, 240, 366, 422, 1554
BssMI	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
BssNI	GRCGYC	1 cut(s)	678
BssT1I	CCWWGG	3 cut(s)	66, 240, 366
Bst2UI	CCWGG	2 cut(s)	327, 1556
Bst4CI	ACNGT	5 cut(s)	316, 568, 610, 688, 1171
Bst6I	CTCTTC	2 cut(s)	428, 1072
BstACI	GRCGYC	1 cut(s)	678
BstAPI	GCANNNNNTGC	2 cut(s)	588, 1465
BstC8I	GCNNGC	5 cut(s)	20, 363, 666, 837, 1606
BstDEI	CTNAG	7 cut(s)	159, 204, 552, 826, 1133, 1401, 1732
BstDSI	CCRYGG	5 cut(s)	66, 193, 240, 366, 422
BstEII	GGTNACC	2 cut(s)	281, 695
BstF5I	GGATG	5 cut(s)	71, 376, 945, 1295, 1633
BstFNI	CGCG	1 cut(s)	195
BstHHI	GCGC	3 cut(s)	499, 670, 1260
BstKTI	GATC	6 cut(s)	261, 325, 1057, 1087, 1400, 1750
BstMAI	GTCTC	2 cut(s)	705, 1219
BstMBI	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
BstMCI	CGRYCG	2 cut(s)	199, 273
BstNI	CCWGG	2 cut(s)	327, 1556
BstPI	GGTNACC	2 cut(s)	281, 695
BstSCI	CCNGG	3 cut(s)	251, 325, 1554
BstUI	CGCG	1 cut(s)	195
BstV1I	GCAGC	9 cut(s)	166, 181, 405, 465, 793, 1443, 1498, 1607, 1706
BstX2I	RGATCY	2 cut(s)	258, 322
BstXI	CCANNNNNNTGG	1 cut(s)	67
BstYI	RGATCY	2 cut(s)	258, 322
Bsu36I	CCTNAGG	1 cut(s)	159
BtgI	CCRYGG	5 cut(s)	66, 193, 240, 366, 422
BtsCI	GGATG	5 cut(s)	71, 376, 945, 1295, 1633
BtsI	GCAGTG	2 cut(s)	463, 1089
BtsIMutI	CAGTG	5 cut(s)	463, 564, 651, 729, 1089
BveI	ACCTGC	1 cut(s)	511
Cac8I	GCNNGC	5 cut(s)	20, 363, 666, 837, 1606
CfoI	GCGC	3 cut(s)	499, 670, 1260
Cfr10I	RCCGGY	3 cut(s)	79, 88, 361
Cfr13I	GGNCC	5 cut(s)	82, 244, 302, 833, 1507
Cfr42I	CCGCGG	1 cut(s)	196
CseI	GACGC	2 cut(s)	686, 850
Csp6I	GTAC	3 cut(s)	393, 442, 556
CspCI	CAANNNNNGTGG	2 cut(s)	520, 555
CviQI	GTAC	3 cut(s)	393, 442, 556
DdeI	CTNAG	7 cut(s)	159, 204, 552, 826, 1133, 1401, 1732
DpnI	GATC	6 cut(s)	260, 324, 1056, 1086, 1399, 1749
DpnII	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
DraI	TTTAAA	1 cut(s)	1569
DraIII	CACNNNGTG	2 cut(s)	505, 588
EaeI	YGGCCR	3 cut(s)	99, 363, 561
Eam1104I	CTCTTC	2 cut(s)	428, 1072
EarI	CTCTTC	2 cut(s)	428, 1072
Eco130I	CCWWGG	3 cut(s)	66, 240, 366
Eco147I	AGGCCT	1 cut(s)	164
Eco32I	GATATC	1 cut(s)	1126
Eco47I	GGWCC	1 cut(s)	1507
Eco57I	CTGAAG	2 cut(s)	1133, 1613
Eco81I	CCTNAGG	1 cut(s)	159
Eco88I	CYCGRG	2 cut(s)	233, 448
Eco91I	GGTNACC	2 cut(s)	281, 695
EcoO109I	RGGNCCY	1 cut(s)	1507
EcoO65I	GGTNACC	2 cut(s)	281, 695
EcoRII	CCWGG	2 cut(s)	325, 1554
EcoRV	GATATC	1 cut(s)	1126
EcoT14I	CCWWGG	3 cut(s)	66, 240, 366
ErhI	CCWWGG	3 cut(s)	66, 240, 366
FaqI	GGGAC	1 cut(s)	865
FauI	CCCGC	3 cut(s)	64, 93, 338
FauNDI	CATATG	1 cut(s)	763
FbaI	TGATCA	1 cut(s)	1054
FblI	GTMKAC	3 cut(s)	199, 273, 776
FokI	GGATG	5 cut(s)	58, 383, 932, 1282, 1640
FspAI	RTGCGCAY	1 cut(s)	498
FspBI	CTAG	2 cut(s)	47, 1703
FspI	TGCGCA	2 cut(s)	498, 1259
GlaI	GCGC	3 cut(s)	498, 669, 1259
GsaI	CCCAGC	1 cut(s)	1319
GsuI	CTGGAG	1 cut(s)	1251
HapII	CCGG	5 cut(s)	80, 89, 253, 362, 1097
HgaI	GACGC	2 cut(s)	686, 850
HhaI	GCGC	3 cut(s)	499, 670, 1260
Hin1I	GRCGYC	1 cut(s)	678
Hin6I	GCGC	3 cut(s)	497, 668, 1258
HinP1I	GCGC	3 cut(s)	497, 668, 1258
HincII	GTYRAC	4 cut(s)	115, 200, 274, 1549
HindII	GTYRAC	4 cut(s)	115, 200, 274, 1549
HindIII	AAGCTT	1 cut(s)	1439
HinfI	GANTC	2 cut(s)	754, 1100
HpaI	GTTAAC	1 cut(s)	1549
HpaII	CCGG	5 cut(s)	80, 89, 253, 362, 1097
HphI	GGTGA	5 cut(s)	293, 341, 391, 633, 1467
Hpy166II	GTNNAC	5 cut(s)	115, 200, 274, 777, 1549
Hpy188III	TCNNGA	7 cut(s)	532, 674, 744, 819, 1022, 1097, 1703
Hpy8I	GTNNAC	5 cut(s)	115, 200, 274, 777, 1549
Hpy99I	CGWCG	4 cut(s)	100, 122, 866, 1496
HpyAV	CCTTC	6 cut(s)	176, 258, 272, 376, 1054, 1350
HpyCH4III	ACNGT	5 cut(s)	316, 568, 610, 688, 1171
HpyCH4IV	ACGT	2 cut(s)	120, 138
HpyCH4V	TGCA	7 cut(s)	796, 899, 940, 1295, 1358, 1459, 1725
HpyF3I	CTNAG	7 cut(s)	159, 204, 552, 826, 1133, 1401, 1732
HpySE526I	ACGT	2 cut(s)	120, 138
Hsp92I	GRCGYC	1 cut(s)	678
HspAI	GCGC	3 cut(s)	497, 668, 1258
Kpn2I	TCCGGA	1 cut(s)	1096
KroI	GCCGGC	1 cut(s)	361
KroNI	GCCGGC	1 cut(s)	363
Ksp22I	TGATCA	1 cut(s)	1054
KspAI	GTTAAC	1 cut(s)	1549
KspI	CCGCGG	1 cut(s)	196
Kzo9I	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
LmnI	GCTCC	6 cut(s)	216, 508, 548, 968, 1099, 1435
Lsp1109I	GCAGC	9 cut(s)	166, 181, 405, 465, 793, 1443, 1498, 1607, 1706
LweI	GCATC	6 cut(s)	80, 361, 793, 886, 1304, 1443
MaeI	CTAG	2 cut(s)	47, 1703
MaeII	ACGT	2 cut(s)	120, 138
MaeIII	GTNAC	8 cut(s)	91, 281, 639, 682, 695, 724, 741, 1205
MalI	GATC	6 cut(s)	260, 324, 1056, 1086, 1399, 1749
MboI	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
MboII	GAAGA	7 cut(s)	445, 905, 1021, 1089, 1385, 1550, 1606
MfeI	CAATTG	1 cut(s)	702
MflI	RGATCY	2 cut(s)	258, 322
MlsI	TGGCCA	1 cut(s)	563
MluNI	TGGCCA	1 cut(s)	563
MmeI	TCCRAC	1 cut(s)	249
Mox20I	TGGCCA	1 cut(s)	563
MroI	TCCGGA	1 cut(s)	1096
MroNI	GCCGGC	1 cut(s)	361
MscI	TGGCCA	1 cut(s)	563
MseI	TTAA	7 cut(s)	813, 843, 885, 1149, 1248, 1548, 1568
MslI	CAYNNNNRTG	2 cut(s)	65, 894
Msp20I	TGGCCA	1 cut(s)	563
MspA1I	CMGCKG	3 cut(s)	195, 1244, 1376
MspI	CCGG	5 cut(s)	80, 89, 253, 362, 1097
MspR9I	CCNGG	3 cut(s)	253, 327, 1556
MunI	CAATTG	1 cut(s)	702
Mva1269I	GAATGC	1 cut(s)	1459
MvaI	CCWGG	2 cut(s)	327, 1556
MvnI	CGCG	1 cut(s)	195
NaeI	GCCGGC	1 cut(s)	363
NciI	CCSGG	1 cut(s)	253
NcoI	CCATGG	3 cut(s)	66, 240, 366
NdeI	CATATG	1 cut(s)	763
NdeII	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
NgoMIV	GCCGGC	1 cut(s)	361
NlaIV	GGNNCC	8 cut(s)	84, 246, 260, 303, 324, 360, 544, 583
NmuCI	GTSAC	4 cut(s)	281, 639, 724, 741
NsbI	TGCGCA	2 cut(s)	498, 1259
PaeR7I	CTCGAG	1 cut(s)	448
PceI	AGGCCT	1 cut(s)	164
PcsI	WCGNNNNNNNCGW	1 cut(s)	126
PctI	GAATGC	1 cut(s)	1459
PdiI	GCCGGC	1 cut(s)	363
PfeI	GAWTC	2 cut(s)	754, 1100
PflMI	CCANNNNNTGG	2 cut(s)	992, 1663
PfoI	TCCNGGA	1 cut(s)	251
PpuMI	RGGWCCY	1 cut(s)	1507
Psp5II	RGGWCCY	1 cut(s)	1507
Psp6I	CCWGG	2 cut(s)	325, 1554
PspEI	GGTNACC	2 cut(s)	281, 695
PspFI	CCCAGC	1 cut(s)	1315
PspGI	CCWGG	2 cut(s)	325, 1554
PspN4I	GGNNCC	8 cut(s)	84, 246, 260, 303, 324, 360, 544, 583
PspPI	GGNCC	5 cut(s)	82, 244, 302, 833, 1507
PspPPI	RGGWCCY	1 cut(s)	1507
PspXI	VCTCGAGB	1 cut(s)	448
PsuI	RGATCY	2 cut(s)	258, 322
PvuII	CAGCTG	1 cut(s)	1376
RsaI	GTAC	3 cut(s)	394, 443, 557
RsaNI	GTAC	3 cut(s)	393, 442, 556
RseI	CAYNNNNRTG	2 cut(s)	65, 894
SacII	CCGCGG	1 cut(s)	196
SalI	GTCGAC	2 cut(s)	198, 272
SaqAI	TTAA	7 cut(s)	813, 843, 885, 1149, 1248, 1548, 1568
Sau3AI	GATC	6 cut(s)	258, 322, 1054, 1084, 1397, 1747
Sau96I	GGNCC	5 cut(s)	82, 244, 302, 833, 1507
ScaI	AGTACT	1 cut(s)	557
ScrFI	CCNGG	3 cut(s)	253, 327, 1556
SfaNI	GCATC	6 cut(s)	80, 361, 793, 886, 1304, 1443
Sfr274I	CTCGAG	1 cut(s)	448
Sfr303I	CCGCGG	1 cut(s)	196
SgrBI	CCGCGG	1 cut(s)	196
SinI	GGWCC	1 cut(s)	1507
SlaI	CTCGAG	1 cut(s)	448
SmiMI	CAYNNNNRTG	2 cut(s)	65, 894
SmlI	CTYRAG	2 cut(s)	448, 530
SmoI	CTYRAG	2 cut(s)	448, 530
SseBI	AGGCCT	1 cut(s)	164
SspI	AATATT	2 cut(s)	907, 1153
SspMI	CTAG	2 cut(s)	47, 1703
StuI	AGGCCT	1 cut(s)	164
StyD4I	CCNGG	3 cut(s)	251, 325, 1554
StyI	CCWWGG	3 cut(s)	66, 240, 366
TaaI	ACNGT	5 cut(s)	316, 568, 610, 688, 1171
TaiI	ACGT	2 cut(s)	123, 141
TaqI	TCGA	5 cut(s)	150, 199, 273, 449, 1396
TatI	WGTACW	2 cut(s)	441, 555
TauI	GCSGC	3 cut(s)	18, 192, 195
TfiI	GAWTC	2 cut(s)	754, 1100
Tru1I	TTAA	7 cut(s)	813, 843, 885, 1149, 1248, 1548, 1568
Tru9I	TTAA	7 cut(s)	813, 843, 885, 1149, 1248, 1548, 1568
TscAI	CASTG	5 cut(s)	463, 571, 651, 729, 1096
TseFI	GTSAC	4 cut(s)	281, 639, 724, 741
TseI	GCWGC	9 cut(s)	154, 169, 418, 453, 806, 1456, 1486, 1620, 1694
Tsp45I	GTSAC	4 cut(s)	281, 639, 724, 741
TspDTI	ATGAA	8 cut(s)	758, 906, 934, 1061, 1332, 1434, 1551, 1644
TspGWI	ACGGA	1 cut(s)	118
TspRI	CASTG	5 cut(s)	463, 571, 651, 729, 1096
Van91I	CCANNNNNTGG	2 cut(s)	992, 1663
VpaK11BI	GGWCC	1 cut(s)	1507
XapI	RAATTY	4 cut(s)	409, 1421, 1631, 1640
XbaI	TCTAGA	1 cut(s)	1702
XcmI	CCANNNNNNNNNTGG	2 cut(s)	373, 1226
XhoI	CTCGAG	1 cut(s)	448
XmiI	GTMKAC	3 cut(s)	199, 273, 776
XspI	CTAG	2 cut(s)	47, 1703
ZrmI	AGTACT	1 cut(s)	557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.