Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Forward (+)

37491145 .. 37498833

Gene Structure 7689 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g50350.t7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1683 bp

ATGGGAGAAACCAAAAGAAAGCGAGGAGCTCCAAAGCCCAAGACCCCCGAAACCGCAATGGACATCGACGAAGCCGCCCCGCCCCCCACGTCGGAGCCTCTCGCCGACGCCGGCACCTCCCAGCCCTCCCGCCGCGGCCGCGGCCGCCCCAGGAAGACCGACTCCGCCAACATCACCGCCTCCCCGGAGCGCCGCAGCTCGCGACACGTGTCGGAGCAGAACGGCGACGCCGCGGAGCCGCCGCTGATGGTGCCGAGCTGGGAGAGCGTGGCGCGCGTGGTGCCGGCGATGGATTCGGTGGTGAAGGTGTTTTGCGTTCACACGGAGCCGAATTTCTCGCTGCCGTGGCAGAGGAAGAGGCAGTATAGCTCGAGTAGCAGTGGGTTTGTGATTGGGGGTAAAAGGGTCTTAACCAATGCGCACTCGGTGGAGCACCATACGCAGGTTAAGCTCAAGAAGCGTGGCTCGGAGACTAAGTACTTGGCTACTGTGTTGGCCATTGGGACTGAATGTGATATTGCCATGCTCACTGTGAGTGATGATGAGTTCTGGGAAGGAGTTTCACCGGTGGAGTTTGGAGAGTTGCCGGCACTACAAGACGCTGTGACGGTTGTGGGCTACCCTATCGGGGGTGACACAATCTCTGTGACAAGTGGTGTTGTGTCACGTATGGAGATACTGTCATATGTTCATGGATCGACTGAGCTTCTGGGACTGCAGATAGATGCTGCCATAAACTCTGGAAACTCTGGTGGGCCTGCCTTCAACGACAAGGGGACCTGTGTAGGCATTGCTTTTCAGTCCCTTAAACACGAAGATGCGGAAAATATAGGCTACGTGATTCCAACACCTGTTATTAAGCACTTCATTACAGATTACGAGAAGAATGGGGCATATACAGGGTTCCCAATTATTGGAATTGAGTGGCAAAAAATGGAAAATCCTGATCTACGCACGTCAATGGGGATGAAGGCTGATCAAAAGGGTGTACGTATCAGAAGGATTGAACCTACAGCTCCAGATTCTCATCTTCTGCAACCATCTGATGTCATTCTTAGCTTCGATAGAGTTAATATTGCAAATGATGGCACAGTTCCATTCAGGCATGGTGAGCGCATAGGTTTCAGTTACCTTGTCTCTCAGAAATATACTGGCGATACTGCTTTAGTGAAAGTTCTTCGTAACTCAAAGATACTGGAATTCAAAATTAAACTTGGAACACACAAGCGTCTGATCCCAGCACACATCAACGGCAAACCTCCTTCCTATTATATCATTGGTGGATTTGTTTTTTCAGCTGTATCTGTTCCGTATCTGCGTTCTGAGTATGGAAAGGATTATGATTTCGACGCTCCCGTCAAACTATTGGACAAACATTTACATGCAATGGCAGAATCTAGTGATGAACAGCTTGTTGTCGTTTCTCAGGTACTTGTTGCTGACATTAATATTGGATATGAGGACATTGTTAACACACAGGTTCTTGCTTTCAATGGTAAGCCTGTGAAAAATCTAAAAAACCTGGCCACCATGGTGGAGAGTTGCGATGAAGAGTACCTAAAGTTTGATCTAGAATACAATCAGATGGTTGCCCTACAGACAAAAACAGCCAAAGCTGCAACGTTAGATATCCTTCTGACTCATTGCATACCTTCAGCCATGTCTGATGATCTTAAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

561

Amino Acids

61.33

Weight (kDa)

6.23

Isoelectric Point (pI)

47.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	4 - 116	6.7e-09	Trypsin
Trypsin_2	PF13365	13 - 90	2.1e-11	Trypsin-like peptidase domain
PDZ_2	PF13180	131 - 231	3.6e-06	PDZ domain
PDZ_3	PF17815	238 - 381	2.7e-45	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1355
Acc16I	TGCGCA	1 cut(s)	420
Acc36I	ACCTGC	1 cut(s)	433
AccB1I	GGYRCC	3 cut(s)	113, 250, 280
AccB7I	CCANNNNNTGG	1 cut(s)	914
AccII	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
AclI	AACGTT	1 cut(s)	1622
AclWI	GGATC	2 cut(s)	703, 1228
AcoI	YGGCCR	4 cut(s)	136, 142, 495, 1524
AcsI	RAATTY	2 cut(s)	331, 1199
AcuI	CTGAAG	1 cut(s)	1638
AcvI	CACGTG	1 cut(s)	208
AcyI	GRCGYC	2 cut(s)	108, 228
AdeI	CACNNNGTG	1 cut(s)	427
AfaI	GTAC	4 cut(s)	479, 990, 1431, 1556
AfiI	CCNNNNNNNGG	5 cut(s)	91, 442, 628, 629, 914
AflII	CTTAAG	1 cut(s)	1673
AflIII	ACRYGT	2 cut(s)	205, 207
AgeI	ACCGGT	1 cut(s)	565
AgsI	TTSAA	4 cut(s)	766, 1007, 1204, 1492
AjiI	CACGTC	2 cut(s)	90, 957
AjnI	CCWGG	2 cut(s)	149, 1521
AleI	CACNNNNGTG	1 cut(s)	1532
Alw21I	GWGCWC	2 cut(s)	31, 435
Alw26I	GTCTC	2 cut(s)	464, 1141
AlwI	GGATC	2 cut(s)	703, 1228
Ama87I	CYCGRG	1 cut(s)	370
AoxI	GGCC	5 cut(s)	136, 142, 495, 755, 1524
ApeKI	GCWGC	4 cut(s)	195, 340, 728, 1616
ApoI	RAATTY	2 cut(s)	331, 1199
AseI	ATTAAT	1 cut(s)	1446
AsiGI	ACCGGT	1 cut(s)	565
AspLEI	GCGC	5 cut(s)	192, 274, 276, 421, 1116
AspS9I	GGNCC	2 cut(s)	755, 777
AsuC2I	CCSGG	1 cut(s)	185
AsuHPI	GGTGA	5 cut(s)	166, 313, 555, 644, 1121
AvaI	CYCGRG	1 cut(s)	370
AvaII	GGWCC	1 cut(s)	777
BalI	TGGCCA	2 cut(s)	497, 1526
BanI	GGYRCC	3 cut(s)	113, 250, 280
BanII	GRGCYC	1 cut(s)	31
BbrPI	CACGTG	1 cut(s)	208
BbsI	GAAGAC	1 cut(s)	161
Bbv12I	GWGCWC	2 cut(s)	31, 435
BbvI	GCAGC	4 cut(s)	207, 327, 715, 1603
BccI	CCATC	5 cut(s)	241, 283, 1048, 1079, 1579
BceAI	ACGGC	3 cut(s)	238, 328, 1267
BciT130I	CCWGG	2 cut(s)	151, 1523
BclI	TGATCA	1 cut(s)	976
BcnI	CCSGG	1 cut(s)	185
BcoDI	GTCTC	2 cut(s)	464, 1141
BfaI	CTAG	2 cut(s)	1398, 1571
BfmI	CTRYAG	3 cut(s)	716, 1011, 1595
BfoI	RGCGCY	1 cut(s)	193
BfrI	CTTAAG	1 cut(s)	1673
BfuAI	ACCTGC	1 cut(s)	433
BmcAI	AGTACT	1 cut(s)	479
Bme1390I	CCNGG	3 cut(s)	151, 185, 1523
Bme18I	GGWCC	1 cut(s)	777
BmeT110I	CYCGRG	1 cut(s)	370
BmgBI	CACGTC	2 cut(s)	90, 957
BmgT120I	GGNCC	2 cut(s)	755, 777
BmiI	GGNNCC	8 cut(s)	96, 115, 237, 252, 282, 327, 778, 905
BmrFI	CCNGG	3 cut(s)	151, 185, 1523
BmsI	GCATC	2 cut(s)	715, 808
BoxI	GACNNNNGTC	1 cut(s)	208
BpiI	GAAGAC	1 cut(s)	161
BpmI	CTGGAG	1 cut(s)	1002
BpuEI	CTTGAG	1 cut(s)	437
BpuMI	CCSGG	1 cut(s)	185
BsaAI	YACGTR	4 cut(s)	208, 668, 838, 992
BsaBI	GATNNNNATC	1 cut(s)	1026
BsaHI	GRCGYC	2 cut(s)	108, 228
BsaJI	CCNNGG	7 cut(s)	133, 139, 149, 183, 231, 344, 1530
BsaWI	WCCGGW	1 cut(s)	565
BsaXI	ACNNNNNCTCC	4 cut(s)	461, 491, 665, 695
Bsc4I	CCNNNNNNNGG	5 cut(s)	91, 442, 628, 629, 914
Bse118I	RCCGGY	4 cut(s)	110, 283, 565, 586
Bse1I	ACTGG	2 cut(s)	1156, 1200
Bse3DI	GCAATG	4 cut(s)	63, 789, 1392, 1642
Bse8I	GATNNNNATC	1 cut(s)	1026
BseBI	CCWGG	2 cut(s)	151, 1523
BseDI	CCNNGG	7 cut(s)	133, 139, 149, 183, 231, 344, 1530
BseGI	GGATG	1 cut(s)	972
BseJI	GATNNNNATC	1 cut(s)	1026
BseLI	CCNNNNNNNGG	5 cut(s)	91, 442, 628, 629, 914
BseMI	GCAATG	4 cut(s)	63, 789, 1392, 1642
BseMII	CTCAG	4 cut(s)	693, 1154, 1314, 1439
BseNI	ACTGG	2 cut(s)	1156, 1200
BsePI	GCGCGC	1 cut(s)	272
BseRI	GAGGAG	1 cut(s)	39
BseX3I	CGGCCG	2 cut(s)	136, 142
BseXI	GCAGC	4 cut(s)	207, 327, 715, 1603
BseYI	CCCAGC	3 cut(s)	120, 258, 1237
Bsh1236I	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
Bsh1285I	CGRYCG	2 cut(s)	139, 145
BshFI	GGCC	5 cut(s)	138, 144, 497, 757, 1526
BshNI	GGYRCC	3 cut(s)	113, 250, 280
BshTI	ACCGGT	1 cut(s)	565
BsiEI	CGRYCG	2 cut(s)	139, 145
BsiHKAI	GWGCWC	2 cut(s)	31, 435
BsiHKCI	CYCGRG	1 cut(s)	370
BsiSI	CCGG	5 cut(s)	111, 185, 284, 566, 587
BslFI	GGGAC	4 cut(s)	517, 726, 787, 790
BslI	CCNNNNNNNGG	5 cut(s)	91, 442, 628, 629, 914
BsmAI	GTCTC	2 cut(s)	464, 1141
BsmFI	GGGAC	4 cut(s)	517, 726, 787, 790
BsnI	GGCC	5 cut(s)	138, 144, 497, 757, 1526
BsoBI	CYCGRG	1 cut(s)	370
Bsp1286I	GDGCHC	2 cut(s)	31, 435
Bsp143I	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
Bsp19I	CCATGG	1 cut(s)	1530
Bsp68I	TCGCGA	1 cut(s)	202
BspANI	GGCC	5 cut(s)	138, 144, 497, 757, 1526
BspCNI	CTCAG	4 cut(s)	694, 1153, 1315, 1438
BspFNI	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
BspLI	GGNNCC	8 cut(s)	96, 115, 237, 252, 282, 327, 778, 905
BspMAI	CTGCAG	1 cut(s)	720
BspMI	ACCTGC	1 cut(s)	433
BspPI	GGATC	2 cut(s)	703, 1228
BspT107I	GGYRCC	3 cut(s)	113, 250, 280
BspTI	CTTAAG	1 cut(s)	1673
BsrDI	GCAATG	4 cut(s)	63, 789, 1392, 1642
BsrFI	RCCGGY	4 cut(s)	110, 283, 565, 586
BsrI	ACTGG	2 cut(s)	1156, 1200
BssAI	RCCGGY	4 cut(s)	110, 283, 565, 586
BssECI	CCNNGG	7 cut(s)	133, 139, 149, 183, 231, 344, 1530
BssHII	GCGCGC	1 cut(s)	272
BssMI	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
BssNI	GRCGYC	2 cut(s)	108, 228
BssT1I	CCWWGG	1 cut(s)	1530
Bst2UI	CCWGG	2 cut(s)	151, 1523
Bst4CI	ACNGT	5 cut(s)	490, 532, 610, 681, 1093
Bst6I	CTCTTC	2 cut(s)	350, 1545
BstACI	GRCGYC	2 cut(s)	108, 228
BstAFI	CTTAAG	1 cut(s)	1673
BstBAI	YACGTR	4 cut(s)	208, 668, 838, 992
BstC8I	GCNNGC	6 cut(s)	112, 200, 274, 285, 588, 759
BstDEI	CTNAG	6 cut(s)	474, 702, 1055, 1140, 1323, 1425
BstDSI	CCRYGG	5 cut(s)	133, 139, 231, 344, 1530
BstF5I	GGATG	1 cut(s)	972
BstFNI	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
BstH2I	RGCGCY	1 cut(s)	193
BstHHI	GCGC	5 cut(s)	192, 274, 276, 421, 1116
BstKTI	GATC	6 cut(s)	698, 949, 979, 1236, 1570, 1672
BstMAI	GTCTC	2 cut(s)	464, 1141
BstMBI	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
BstMCI	CGRYCG	2 cut(s)	139, 145
BstNI	CCWGG	2 cut(s)	151, 1523
BstNSI	RCATGY	1 cut(s)	1385
BstPAI	GACNNNNGTC	1 cut(s)	208
BstSCI	CCNGG	3 cut(s)	149, 183, 1521
BstSFI	CTRYAG	3 cut(s)	716, 1011, 1595
BstSNI	TACGTA	1 cut(s)	992
BstUI	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
BstV1I	GCAGC	4 cut(s)	207, 327, 715, 1603
BstV2I	GAAGAC	1 cut(s)	161
BstXI	CCANNNNNNTGG	1 cut(s)	1534
BstZI	CGGCCG	2 cut(s)	136, 142
BsuRI	GGCC	5 cut(s)	138, 144, 497, 757, 1526
BtgI	CCRYGG	5 cut(s)	133, 139, 231, 344, 1530
BtgZI	GCGATG	2 cut(s)	302, 1560
BtrI	CACGTC	2 cut(s)	90, 957
BtsCI	GGATG	1 cut(s)	972
BtsI	GCAGTG	1 cut(s)	385
BtsIMutI	CAGTG	2 cut(s)	385, 528
BtuMI	TCGCGA	1 cut(s)	202
BveI	ACCTGC	1 cut(s)	433
Cac8I	GCNNGC	6 cut(s)	112, 200, 274, 285, 588, 759
CciNI	GCGGCCGC	2 cut(s)	136, 142
CfoI	GCGC	5 cut(s)	192, 274, 276, 421, 1116
Cfr10I	RCCGGY	4 cut(s)	110, 283, 565, 586
Cfr13I	GGNCC	2 cut(s)	755, 777
Cfr42I	CCGCGG	3 cut(s)	136, 142, 234
CseI	GACGC	5 cut(s)	116, 236, 608, 1217, 1358
Csp6I	GTAC	4 cut(s)	478, 989, 1430, 1555
CspAI	ACCGGT	1 cut(s)	565
CspCI	CAANNNNNGTGG	2 cut(s)	442, 477
CviAII	CATG	6 cut(s)	523, 692, 1106, 1382, 1531, 1660
CviQI	GTAC	4 cut(s)	478, 989, 1430, 1555
DdeI	CTNAG	6 cut(s)	474, 702, 1055, 1140, 1323, 1425
DpnI	GATC	6 cut(s)	697, 948, 978, 1235, 1569, 1671
DpnII	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
DraIII	CACNNNGTG	1 cut(s)	427
DrdI	GACNNNNNNGTC	1 cut(s)	1355
DseDI	GACNNNNNNGTC	1 cut(s)	1355
EaeI	YGGCCR	4 cut(s)	136, 142, 495, 1524
EagI	CGGCCG	2 cut(s)	136, 142
Eam1104I	CTCTTC	2 cut(s)	350, 1545
EarI	CTCTTC	2 cut(s)	350, 1545
EciI	GGCGGA	1 cut(s)	154
Ecl136II	GAGCTC	1 cut(s)	29
EclXI	CGGCCG	2 cut(s)	136, 142
Eco105I	TACGTA	1 cut(s)	992
Eco130I	CCWWGG	1 cut(s)	1530
Eco24I	GRGCYC	1 cut(s)	31
Eco32I	GATATC	1 cut(s)	1630
Eco47I	GGWCC	1 cut(s)	777
Eco52I	CGGCCG	2 cut(s)	136, 142
Eco53kI	GAGCTC	1 cut(s)	29
Eco57I	CTGAAG	1 cut(s)	1638
Eco72I	CACGTG	1 cut(s)	208
Eco88I	CYCGRG	1 cut(s)	370
EcoICRI	GAGCTC	1 cut(s)	29
EcoO109I	RGGNCCY	1 cut(s)	777
EcoRI	GAATTC	1 cut(s)	1199
EcoRII	CCWGG	2 cut(s)	149, 1521
EcoRV	GATATC	1 cut(s)	1630
EcoT14I	CCWWGG	1 cut(s)	1530
EcoT38I	GRGCYC	1 cut(s)	31
ErhI	CCWWGG	1 cut(s)	1530
FaeI	CATG	6 cut(s)	526, 695, 1109, 1385, 1534, 1663
FaqI	GGGAC	4 cut(s)	517, 726, 787, 790
FatI	CATG	6 cut(s)	522, 691, 1105, 1381, 1530, 1659
FauI	CCCGC	2 cut(s)	87, 137
FauNDI	CATATG	1 cut(s)	685
FbaI	TGATCA	1 cut(s)	976
FokI	GGATG	1 cut(s)	979
FriOI	GRGCYC	1 cut(s)	31
FspAI	RTGCGCAY	1 cut(s)	420
FspBI	CTAG	2 cut(s)	1398, 1571
FspI	TGCGCA	1 cut(s)	420
GlaI	GCGC	5 cut(s)	191, 273, 275, 420, 1115
GsaI	CCCAGC	3 cut(s)	124, 262, 1241
GsuI	CTGGAG	1 cut(s)	1002
HaeII	RGCGCY	1 cut(s)	193
HaeIII	GGCC	5 cut(s)	138, 144, 497, 757, 1526
HapII	CCGG	5 cut(s)	111, 185, 284, 566, 587
HgaI	GACGC	5 cut(s)	116, 236, 608, 1217, 1358
HhaI	GCGC	5 cut(s)	192, 274, 276, 421, 1116
Hin1I	GRCGYC	2 cut(s)	108, 228
Hin1II	CATG	6 cut(s)	526, 695, 1109, 1385, 1534, 1663
Hin6I	GCGC	5 cut(s)	190, 272, 274, 419, 1114
HinP1I	GCGC	5 cut(s)	190, 272, 274, 419, 1114
HincII	GTYRAC	1 cut(s)	1471
HindII	GTYRAC	1 cut(s)	1471
HinfI	GANTC	6 cut(s)	161, 293, 841, 1022, 1394, 1639
HpaI	GTTAAC	1 cut(s)	1471
HpaII	CCGG	5 cut(s)	111, 185, 284, 566, 587
HphI	GGTGA	5 cut(s)	166, 313, 555, 644, 1121
Hpy166II	GTNNAC	3 cut(s)	319, 989, 1471
Hpy188III	TCNNGA	6 cut(s)	201, 454, 741, 944, 1019, 1571
Hpy8I	GTNNAC	3 cut(s)	319, 989, 1471
Hpy99I	CGWCG	5 cut(s)	71, 94, 110, 230, 1352
HpyAV	CCTTC	8 cut(s)	298, 548, 772, 964, 993, 1272, 1643, 1662
HpyCH4III	ACNGT	5 cut(s)	490, 532, 610, 681, 1093
HpyCH4IV	ACGT	7 cut(s)	89, 207, 667, 837, 956, 991, 1622
HpyCH4V	TGCA	6 cut(s)	718, 1036, 1079, 1385, 1619, 1647
HpyF3I	CTNAG	6 cut(s)	474, 702, 1055, 1140, 1323, 1425
HpySE526I	ACGT	7 cut(s)	89, 207, 667, 837, 956, 991, 1622
Hsp92I	GRCGYC	2 cut(s)	108, 228
Hsp92II	CATG	6 cut(s)	526, 695, 1109, 1385, 1534, 1663
HspAI	GCGC	5 cut(s)	190, 272, 274, 419, 1114
KroI	GCCGGC	3 cut(s)	110, 283, 586
KroNI	GCCGGC	3 cut(s)	112, 285, 588
Ksp22I	TGATCA	1 cut(s)	976
KspAI	GTTAAC	1 cut(s)	1471
KspI	CCGCGG	3 cut(s)	136, 142, 234
Kzo9I	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
Lsp1109I	GCAGC	4 cut(s)	207, 327, 715, 1603
LweI	GCATC	2 cut(s)	715, 808
MaeI	CTAG	2 cut(s)	1398, 1571
MaeII	ACGT	7 cut(s)	89, 207, 667, 837, 956, 991, 1622
MaeIII	GTNAC	6 cut(s)	604, 632, 646, 663, 1127, 1181
MalI	GATC	6 cut(s)	697, 948, 978, 1235, 1569, 1671
MboI	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
MboII	GAAGA	7 cut(s)	166, 367, 827, 895, 1022, 1169, 1562
MhlI	GDGCHC	2 cut(s)	31, 435
MlsI	TGGCCA	2 cut(s)	497, 1526
MluCI	AATT	5 cut(s)	331, 909, 918, 1199, 1206
MluNI	TGGCCA	2 cut(s)	497, 1526
MlyI	GAGTC	2 cut(s)	155, 1633
MmeI	TCCRAC	3 cut(s)	72, 192, 869
MnlI	CCTC	9 cut(s)	17, 108, 127, 136, 190, 345, 351, 1269, 1453
Mox20I	TGGCCA	2 cut(s)	497, 1526
MroNI	GCCGGC	3 cut(s)	110, 283, 586
MscI	TGGCCA	2 cut(s)	497, 1526
MslI	CAYNNNNRTG	4 cut(s)	816, 959, 1380, 1532
Msp20I	TGGCCA	2 cut(s)	497, 1526
MspA1I	CMGCKG	5 cut(s)	135, 141, 233, 244, 1298
MspCI	CTTAAG	1 cut(s)	1673
MspI	CCGG	5 cut(s)	111, 185, 284, 566, 587
MspR9I	CCNGG	3 cut(s)	151, 185, 1523
MvaI	CCWGG	2 cut(s)	151, 1523
MvnI	CGCG	6 cut(s)	135, 141, 202, 233, 274, 276
NaeI	GCCGGC	3 cut(s)	112, 285, 588
NciI	CCSGG	1 cut(s)	185
NcoI	CCATGG	1 cut(s)	1530
NdeI	CATATG	1 cut(s)	685
NdeII	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
NgoMIV	GCCGGC	3 cut(s)	110, 283, 586
NlaIII	CATG	6 cut(s)	526, 695, 1109, 1385, 1534, 1663
NlaIV	GGNNCC	8 cut(s)	96, 115, 237, 252, 282, 327, 778, 905
NmeAIII	GCCGAG	1 cut(s)	279
NmuCI	GTSAC	4 cut(s)	604, 632, 646, 663
NotI	GCGGCCGC	2 cut(s)	136, 142
NruI	TCGCGA	1 cut(s)	202
NsbI	TGCGCA	1 cut(s)	420
NspI	RCATGY	1 cut(s)	1385
OliI	CACNNNNGTG	1 cut(s)	1532
PaeR7I	CTCGAG	1 cut(s)	370
PauI	GCGCGC	1 cut(s)	272
PcsI	WCGNNNNNNNCGW	1 cut(s)	1353
PdiI	GCCGGC	3 cut(s)	112, 285, 588
PfeI	GAWTC	4 cut(s)	293, 841, 1022, 1394
PflMI	CCANNNNNTGG	1 cut(s)	914
PinAI	ACCGGT	1 cut(s)	565
PleI	GAGTC	2 cut(s)	155, 1633
PmaCI	CACGTG	1 cut(s)	208
PmlI	CACGTG	1 cut(s)	208
PpsI	GAGTC	2 cut(s)	155, 1633
Ppu21I	YACGTR	4 cut(s)	208, 668, 838, 992
PpuMI	RGGWCCY	1 cut(s)	777
PshAI	GACNNNNGTC	1 cut(s)	208
PshBI	ATTAAT	1 cut(s)	1446
Psp124BI	GAGCTC	1 cut(s)	31
Psp1406I	AACGTT	1 cut(s)	1622
Psp5II	RGGWCCY	1 cut(s)	777
Psp6I	CCWGG	2 cut(s)	149, 1521
PspCI	CACGTG	1 cut(s)	208
PspFI	CCCAGC	3 cut(s)	120, 258, 1237
PspGI	CCWGG	2 cut(s)	149, 1521
PspN4I	GGNNCC	8 cut(s)	96, 115, 237, 252, 282, 327, 778, 905
PspPI	GGNCC	2 cut(s)	755, 777
PspPPI	RGGWCCY	1 cut(s)	777
PspXI	VCTCGAGB	1 cut(s)	370
PstI	CTGCAG	1 cut(s)	720
PteI	GCGCGC	1 cut(s)	272
PvuII	CAGCTG	1 cut(s)	1298
RruI	TCGCGA	1 cut(s)	202
RsaI	GTAC	4 cut(s)	479, 990, 1431, 1556
RsaNI	GTAC	4 cut(s)	478, 989, 1430, 1555
RseI	CAYNNNNRTG	4 cut(s)	816, 959, 1380, 1532
SacI	GAGCTC	1 cut(s)	31
SacII	CCGCGG	3 cut(s)	136, 142, 234
Sau3AI	GATC	6 cut(s)	695, 946, 976, 1233, 1567, 1669
Sau96I	GGNCC	2 cut(s)	755, 777
ScaI	AGTACT	1 cut(s)	479
SchI	GAGTC	2 cut(s)	155, 1633
ScrFI	CCNGG	3 cut(s)	151, 185, 1523
SduI	GDGCHC	2 cut(s)	31, 435
SfaNI	GCATC	2 cut(s)	715, 808
SfcI	CTRYAG	3 cut(s)	716, 1011, 1595
Sfr274I	CTCGAG	1 cut(s)	370
Sfr303I	CCGCGG	3 cut(s)	136, 142, 234
SgrAI	CRCCGGYG	1 cut(s)	565
SgrBI	CCGCGG	3 cut(s)	136, 142, 234
SinI	GGWCC	1 cut(s)	777
SlaI	CTCGAG	1 cut(s)	370
SmiMI	CAYNNNNRTG	4 cut(s)	816, 959, 1380, 1532
SmlI	CTYRAG	3 cut(s)	370, 452, 1673
SmoI	CTYRAG	3 cut(s)	370, 452, 1673
SnaBI	TACGTA	1 cut(s)	992
Sse9I	AATT	5 cut(s)	331, 909, 918, 1199, 1206
SspI	AATATT	2 cut(s)	1075, 1450
SspMI	CTAG	2 cut(s)	1398, 1571
SstI	GAGCTC	1 cut(s)	31
StyD4I	CCNGG	3 cut(s)	149, 183, 1521
StyI	CCWWGG	1 cut(s)	1530
TaaI	ACNGT	5 cut(s)	490, 532, 610, 681, 1093
TaiI	ACGT	7 cut(s)	92, 210, 670, 840, 959, 994, 1625
TaqI	TCGA	5 cut(s)	66, 371, 698, 1062, 1347
TaqII	GACCGA	1 cut(s)	173
TasI	AATT	5 cut(s)	331, 909, 918, 1199, 1206
TatI	WGTACW	1 cut(s)	477
TfiI	GAWTC	4 cut(s)	293, 841, 1022, 1394
TscAI	CASTG	2 cut(s)	385, 535
TseFI	GTSAC	4 cut(s)	604, 632, 646, 663
TseI	GCWGC	4 cut(s)	195, 340, 728, 1616
Tsp45I	GTSAC	4 cut(s)	604, 632, 646, 663
TspDTI	ATGAA	5 cut(s)	680, 856, 983, 1419, 1563
TspGWI	ACGGA	2 cut(s)	338, 1299
TspRI	CASTG	2 cut(s)	385, 535
Van91I	CCANNNNNTGG	1 cut(s)	914
Vha464I	CTTAAG	1 cut(s)	1673
VpaK11BI	GGWCC	1 cut(s)	777
VspI	ATTAAT	1 cut(s)	1446
XapI	RAATTY	2 cut(s)	331, 1199
XbaI	TCTAGA	1 cut(s)	1570
XceI	RCATGY	1 cut(s)	1385
XhoI	CTCGAG	1 cut(s)	370
XspI	CTAG	2 cut(s)	1398, 1571
ZrmI	AGTACT	1 cut(s)	479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.