protease Do-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1184 |
| AccB1I | GGYRCC | 2 cut(s) | 283, 506 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 917, 1588 |
| AccII | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| AclWI | GGATC | 4 cut(s) | 242, 255, 1004, 1317 |
| AcoI | YGGCCR | 5 cut(s) | 172, 178, 237, 486, 1527 |
| AcsI | RAATTY | 2 cut(s) | 334, 1565 |
| AcuI | CTGAAG | 1 cut(s) | 218 |
| AcyI | GRCGYC | 1 cut(s) | 117 |
| AdeI | CACNNNGTG | 2 cut(s) | 430, 513 |
| AfaI | GTAC | 3 cut(s) | 368, 482, 958 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 170, 632, 917, 1588 |
| AgeI | ACCGGT | 1 cut(s) | 568 |
| AgsI | TTSAA | 4 cut(s) | 1039, 1352, 1564, 1678 |
| AjnI | CCWGG | 2 cut(s) | 260, 444 |
| Alw21I | GWGCWC | 1 cut(s) | 414 |
| Alw26I | GTCTC | 2 cut(s) | 568, 630 |
| AlwI | GGATC | 4 cut(s) | 242, 255, 1004, 1317 |
| Ama87I | CYCGRG | 2 cut(s) | 373, 751 |
| AoxI | GGCC | 8 cut(s) | 25, 172, 178, 237, 258, 486, 758, 1527 |
| ApeKI | GCWGC | 8 cut(s) | 343, 352, 378, 595, 731, 1381, 1411, 1619 |
| ApoI | RAATTY | 2 cut(s) | 334, 1565 |
| AsiGI | ACCGGT | 1 cut(s) | 568 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 311 |
| AspLEI | GCGC | 3 cut(s) | 465, 595, 1185 |
| AspS9I | GGNCC | 1 cut(s) | 758 |
| AsuC2I | CCSGG | 2 cut(s) | 236, 252 |
| AsuHPI | GGTGA | 4 cut(s) | 316, 406, 558, 1392 |
| AvaI | CYCGRG | 2 cut(s) | 373, 751 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1287, 1320 |
| BalI | TGGCCA | 2 cut(s) | 488, 1529 |
| BamHI | GGATCC | 1 cut(s) | 247 |
| BanI | GGYRCC | 2 cut(s) | 283, 506 |
| BbsI | GAAGAC | 1 cut(s) | 1470 |
| Bbv12I | GWGCWC | 1 cut(s) | 414 |
| BbvI | GCAGC | 8 cut(s) | 330, 364, 390, 582, 718, 1368, 1423, 1631 |
| BccI | CCATC | 5 cut(s) | 286, 1051, 1061, 1082, 1582 |
| BceAI | ACGGC | 6 cut(s) | 12, 104, 229, 271, 273, 331 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 265, 299 |
| BciT130I | CCWGG | 2 cut(s) | 262, 446 |
| BclI | TGATCA | 1 cut(s) | 979 |
| BcnI | CCSGG | 2 cut(s) | 236, 252 |
| BcoDI | GTCTC | 2 cut(s) | 568, 630 |
| BfaI | CTAG | 3 cut(s) | 59, 713, 1628 |
| BmcAI | AGTACT | 1 cut(s) | 482 |
| Bme1390I | CCNGG | 4 cut(s) | 236, 252, 262, 446 |
| BmeT110I | CYCGRG | 2 cut(s) | 373, 751 |
| BmgT120I | GGNCC | 1 cut(s) | 758 |
| BmiI | GGNNCC | 4 cut(s) | 249, 285, 469, 508 |
| BmrFI | CCNGG | 4 cut(s) | 236, 252, 262, 446 |
| BmrI | ACTGGG | 1 cut(s) | 1164 |
| BmsI | GCATC | 5 cut(s) | 83, 592, 718, 811, 1229 |
| BmuI | ACTGGG | 1 cut(s) | 1164 |
| BpiI | GAAGAC | 1 cut(s) | 1470 |
| BpuEI | CTTGAG | 1 cut(s) | 440 |
| BpuMI | CCSGG | 2 cut(s) | 236, 252 |
| BsaBI | GATNNNNATC | 1 cut(s) | 246 |
| BsaHI | GRCGYC | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 6 cut(s) | 169, 175, 234, 261, 347, 444 |
| BsaWI | WCCGGW | 2 cut(s) | 568, 1402 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 170, 632, 917, 1588 |
| Bse118I | RCCGGY | 1 cut(s) | 568 |
| Bse1I | ACTGG | 2 cut(s) | 854, 1159 |
| Bse3DI | GCAATG | 3 cut(s) | 519, 531, 1668 |
| Bse8I | GATNNNNATC | 1 cut(s) | 246 |
| BseBI | CCWGG | 2 cut(s) | 262, 446 |
| BseDI | CCNNGG | 6 cut(s) | 169, 175, 234, 261, 347, 444 |
| BseGI | GGATG | 6 cut(s) | 74, 92, 826, 1220, 1411, 1558 |
| BseJI | GATNNNNATC | 1 cut(s) | 246 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 170, 632, 917, 1588 |
| BseMI | GCAATG | 3 cut(s) | 519, 531, 1668 |
| BseMII | CTCAG | 7 cut(s) | 141, 441, 1043, 1072, 1182, 1213, 1442 |
| BseNI | ACTGG | 2 cut(s) | 854, 1159 |
| BseX3I | CGGCCG | 3 cut(s) | 172, 178, 237 |
| BseXI | GCAGC | 8 cut(s) | 330, 364, 390, 582, 718, 1368, 1423, 1631 |
| BsgI | GTGCAG | 1 cut(s) | 301 |
| Bsh1236I | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| Bsh1285I | CGRYCG | 3 cut(s) | 175, 181, 240 |
| BshFI | GGCC | 8 cut(s) | 27, 174, 180, 239, 260, 488, 760, 1529 |
| BshNI | GGYRCC | 2 cut(s) | 283, 506 |
| BshTI | ACCGGT | 1 cut(s) | 568 |
| BsiEI | CGRYCG | 3 cut(s) | 175, 181, 240 |
| BsiHKAI | GWGCWC | 1 cut(s) | 414 |
| BsiHKCI | CYCGRG | 2 cut(s) | 373, 751 |
| BsiSI | CCGG | 4 cut(s) | 236, 252, 569, 1403 |
| BslFI | GGGAC | 1 cut(s) | 790 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 170, 632, 917, 1588 |
| BsmAI | GTCTC | 2 cut(s) | 568, 630 |
| BsmFI | GGGAC | 1 cut(s) | 790 |
| BsmI | GAATGC | 1 cut(s) | 1384 |
| BsnI | GGCC | 8 cut(s) | 27, 174, 180, 239, 260, 488, 760, 1529 |
| BsoBI | CYCGRG | 2 cut(s) | 373, 751 |
| Bsp1286I | GDGCHC | 1 cut(s) | 414 |
| Bsp143I | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| BspANI | GGCC | 8 cut(s) | 27, 174, 180, 239, 260, 488, 760, 1529 |
| BspCNI | CTCAG | 7 cut(s) | 140, 440, 1042, 1071, 1183, 1212, 1441 |
| BspFNI | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| BspLI | GGNNCC | 4 cut(s) | 249, 285, 469, 508 |
| BspPI | GGATC | 4 cut(s) | 242, 255, 1004, 1317 |
| BspT107I | GGYRCC | 2 cut(s) | 283, 506 |
| BsrDI | GCAATG | 3 cut(s) | 519, 531, 1668 |
| BsrFI | RCCGGY | 1 cut(s) | 568 |
| BsrI | ACTGG | 2 cut(s) | 854, 1159 |
| BssAI | RCCGGY | 1 cut(s) | 568 |
| BssECI | CCNNGG | 6 cut(s) | 169, 175, 234, 261, 347, 444 |
| BssMI | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| BssNI | GRCGYC | 1 cut(s) | 117 |
| Bst2UI | CCWGG | 2 cut(s) | 262, 446 |
| Bst4CI | ACNGT | 6 cut(s) | 493, 535, 613, 1096, 1547, 1656 |
| BstACI | GRCGYC | 1 cut(s) | 117 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1390 |
| BstC8I | GCNNGC | 5 cut(s) | 231, 320, 591, 762, 1531 |
| BstDEI | CTNAG | 7 cut(s) | 127, 427, 1029, 1058, 1191, 1199, 1428 |
| BstDSI | CCRYGG | 3 cut(s) | 169, 175, 347 |
| BstF5I | GGATG | 6 cut(s) | 74, 92, 826, 1220, 1411, 1558 |
| BstFNI | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| BstHHI | GCGC | 3 cut(s) | 465, 595, 1185 |
| BstKTI | GATC | 5 cut(s) | 250, 738, 982, 1012, 1325 |
| BstMAI | GTCTC | 2 cut(s) | 568, 630 |
| BstMBI | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| BstMCI | CGRYCG | 3 cut(s) | 175, 181, 240 |
| BstNI | CCWGG | 2 cut(s) | 262, 446 |
| BstSCI | CCNGG | 4 cut(s) | 234, 250, 260, 444 |
| BstUI | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| BstV1I | GCAGC | 8 cut(s) | 330, 364, 390, 582, 718, 1368, 1423, 1631 |
| BstV2I | GAAGAC | 1 cut(s) | 1470 |
| BstX2I | RGATCY | 1 cut(s) | 247 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 484, 1021 |
| BstYI | RGATCY | 1 cut(s) | 247 |
| BstZI | CGGCCG | 3 cut(s) | 172, 178, 237 |
| BsuRI | GGCC | 8 cut(s) | 27, 174, 180, 239, 260, 488, 760, 1529 |
| BtgI | CCRYGG | 3 cut(s) | 169, 175, 347 |
| BtsCI | GGATG | 6 cut(s) | 74, 92, 826, 1220, 1411, 1558 |
| BtsI | GCAGTG | 3 cut(s) | 388, 1014, 1645 |
| BtsIMutI | CAGTG | 6 cut(s) | 388, 435, 489, 654, 1014, 1645 |
| Cac8I | GCNNGC | 5 cut(s) | 231, 320, 591, 762, 1531 |
| CciNI | GCGGCCGC | 2 cut(s) | 172, 178 |
| CfoI | GCGC | 3 cut(s) | 465, 595, 1185 |
| Cfr10I | RCCGGY | 1 cut(s) | 568 |
| Cfr13I | GGNCC | 1 cut(s) | 758 |
| Cfr42I | CCGCGG | 2 cut(s) | 172, 178 |
| CseI | GACGC | 2 cut(s) | 125, 305 |
| Csp6I | GTAC | 3 cut(s) | 367, 481, 957 |
| CspAI | ACCGGT | 1 cut(s) | 568 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 478, 513 |
| CviAII | CATG | 5 cut(s) | 695, 815, 970, 1109, 1534 |
| CviQI | GTAC | 3 cut(s) | 367, 481, 957 |
| DdeI | CTNAG | 7 cut(s) | 127, 427, 1029, 1058, 1191, 1199, 1428 |
| DpnI | GATC | 5 cut(s) | 249, 737, 981, 1011, 1324 |
| DpnII | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| DraIII | CACNNNGTG | 2 cut(s) | 430, 513 |
| EaeI | YGGCCR | 5 cut(s) | 172, 178, 237, 486, 1527 |
| EagI | CGGCCG | 3 cut(s) | 172, 178, 237 |
| EciI | GGCGGA | 1 cut(s) | 85 |
| EclXI | CGGCCG | 3 cut(s) | 172, 178, 237 |
| Eco32I | GATATC | 1 cut(s) | 1447 |
| Eco52I | CGGCCG | 3 cut(s) | 172, 178, 237 |
| Eco57I | CTGAAG | 1 cut(s) | 218 |
| Eco88I | CYCGRG | 2 cut(s) | 373, 751 |
| EcoRII | CCWGG | 2 cut(s) | 260, 444 |
| EcoRV | GATATC | 1 cut(s) | 1447 |
| FaeI | CATG | 5 cut(s) | 698, 818, 973, 1112, 1537 |
| FaqI | GGGAC | 1 cut(s) | 790 |
| FatI | CATG | 5 cut(s) | 694, 814, 969, 1108, 1533 |
| FauI | CCCGC | 4 cut(s) | 173, 234, 238, 263 |
| FbaI | TGATCA | 1 cut(s) | 979 |
| FokI | GGATG | 6 cut(s) | 61, 79, 833, 1207, 1418, 1565 |
| FspBI | CTAG | 3 cut(s) | 59, 713, 1628 |
| FspI | TGCGCA | 1 cut(s) | 1184 |
| GlaI | GCGC | 3 cut(s) | 464, 594, 1184 |
| HaeIII | GGCC | 8 cut(s) | 27, 174, 180, 239, 260, 488, 760, 1529 |
| HapII | CCGG | 4 cut(s) | 236, 252, 569, 1403 |
| HgaI | GACGC | 2 cut(s) | 125, 305 |
| HhaI | GCGC | 3 cut(s) | 465, 595, 1185 |
| Hin1I | GRCGYC | 1 cut(s) | 117 |
| Hin1II | CATG | 5 cut(s) | 698, 818, 973, 1112, 1537 |
| Hin6I | GCGC | 3 cut(s) | 463, 593, 1183 |
| HinP1I | GCGC | 3 cut(s) | 463, 593, 1183 |
| HincII | GTYRAC | 2 cut(s) | 702, 1474 |
| HindII | GTYRAC | 2 cut(s) | 702, 1474 |
| HindIII | AAGCTT | 1 cut(s) | 1522 |
| HinfI | GANTC | 6 cut(s) | 242, 679, 1025, 1195, 1483, 1513 |
| HpaI | GTTAAC | 1 cut(s) | 1474 |
| HpaII | CCGG | 4 cut(s) | 236, 252, 569, 1403 |
| HphI | GGTGA | 4 cut(s) | 316, 406, 558, 1392 |
| Hpy166II | GTNNAC | 2 cut(s) | 702, 1474 |
| Hpy188III | TCNNGA | 6 cut(s) | 142, 196, 457, 744, 947, 1022 |
| Hpy8I | GTNNAC | 2 cut(s) | 702, 1474 |
| Hpy99I | CGWCG | 4 cut(s) | 110, 119, 143, 292 |
| HpyAV | CCTTC | 3 cut(s) | 301, 551, 1275 |
| HpyCH4III | ACNGT | 6 cut(s) | 493, 535, 613, 1096, 1547, 1656 |
| HpyCH4IV | ACGT | 1 cut(s) | 138 |
| HpyF3I | CTNAG | 7 cut(s) | 127, 427, 1029, 1058, 1191, 1199, 1428 |
| HpySE526I | ACGT | 1 cut(s) | 138 |
| Hsp92I | GRCGYC | 1 cut(s) | 117 |
| Hsp92II | CATG | 5 cut(s) | 698, 818, 973, 1112, 1537 |
| HspAI | GCGC | 3 cut(s) | 463, 593, 1183 |
| Ksp22I | TGATCA | 1 cut(s) | 979 |
| KspAI | GTTAAC | 1 cut(s) | 1474 |
| KspI | CCGCGG | 2 cut(s) | 172, 178 |
| Kzo9I | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| LmnI | GCTCC | 5 cut(s) | 198, 433, 473, 893, 1360 |
| Lsp1109I | GCAGC | 8 cut(s) | 330, 364, 390, 582, 718, 1368, 1423, 1631 |
| LweI | GCATC | 5 cut(s) | 83, 592, 718, 811, 1229 |
| MaeI | CTAG | 3 cut(s) | 59, 713, 1628 |
| MaeII | ACGT | 1 cut(s) | 138 |
| MaeIII | GTNAC | 5 cut(s) | 515, 607, 649, 1130, 1398 |
| MalI | GATC | 5 cut(s) | 249, 737, 981, 1011, 1324 |
| MboI | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| MboII | GAAGA | 6 cut(s) | 898, 1014, 1046, 1310, 1475, 1522 |
| MflI | RGATCY | 1 cut(s) | 247 |
| MhlI | GDGCHC | 1 cut(s) | 414 |
| MlsI | TGGCCA | 2 cut(s) | 488, 1529 |
| MluCI | AATT | 7 cut(s) | 334, 642, 912, 1005, 1186, 1236, 1565 |
| MluNI | TGGCCA | 2 cut(s) | 488, 1529 |
| Mox20I | TGGCCA | 2 cut(s) | 488, 1529 |
| MroXI | GAANNNNTTC | 1 cut(s) | 311 |
| MscI | TGGCCA | 2 cut(s) | 488, 1529 |
| MseI | TTAA | 5 cut(s) | 768, 1074, 1173, 1473, 1494 |
| MslI | CAYNNNNRTG | 2 cut(s) | 524, 819 |
| Msp20I | TGGCCA | 2 cut(s) | 488, 1529 |
| MspA1I | CMGCKG | 3 cut(s) | 171, 177, 1301 |
| MspI | CCGG | 4 cut(s) | 236, 252, 569, 1403 |
| MspR9I | CCNGG | 4 cut(s) | 236, 252, 262, 446 |
| Mva1269I | GAATGC | 1 cut(s) | 1384 |
| MvaI | CCWGG | 2 cut(s) | 262, 446 |
| MvnI | CGCG | 5 cut(s) | 171, 177, 299, 465, 670 |
| NciI | CCSGG | 2 cut(s) | 236, 252 |
| NdeII | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| NlaIII | CATG | 5 cut(s) | 698, 818, 973, 1112, 1537 |
| NlaIV | GGNNCC | 4 cut(s) | 249, 285, 469, 508 |
| NmuCI | GTSAC | 3 cut(s) | 515, 649, 1398 |
| NotI | GCGGCCGC | 2 cut(s) | 172, 178 |
| NsbI | TGCGCA | 1 cut(s) | 1184 |
| PaeR7I | CTCGAG | 1 cut(s) | 373 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 111, 147, 219 |
| PctI | GAATGC | 1 cut(s) | 1384 |
| PdmI | GAANNNNTTC | 1 cut(s) | 311 |
| PfeI | GAWTC | 6 cut(s) | 242, 679, 1025, 1195, 1483, 1513 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 917, 1588 |
| PfoI | TCCNGGA | 1 cut(s) | 250 |
| PinAI | ACCGGT | 1 cut(s) | 568 |
| Psp6I | CCWGG | 2 cut(s) | 260, 444 |
| PspGI | CCWGG | 2 cut(s) | 260, 444 |
| PspN4I | GGNNCC | 4 cut(s) | 249, 285, 469, 508 |
| PspPI | GGNCC | 1 cut(s) | 758 |
| PspXI | VCTCGAGB | 1 cut(s) | 373 |
| PsuI | RGATCY | 1 cut(s) | 247 |
| PvuII | CAGCTG | 1 cut(s) | 1301 |
| RsaI | GTAC | 3 cut(s) | 368, 482, 958 |
| RsaNI | GTAC | 3 cut(s) | 367, 481, 957 |
| RseI | CAYNNNNRTG | 2 cut(s) | 524, 819 |
| SacII | CCGCGG | 2 cut(s) | 172, 178 |
| SaqAI | TTAA | 5 cut(s) | 768, 1074, 1173, 1473, 1494 |
| Sau3AI | GATC | 5 cut(s) | 247, 735, 979, 1009, 1322 |
| Sau96I | GGNCC | 1 cut(s) | 758 |
| ScaI | AGTACT | 1 cut(s) | 482 |
| ScrFI | CCNGG | 4 cut(s) | 236, 252, 262, 446 |
| SduI | GDGCHC | 1 cut(s) | 414 |
| SfaNI | GCATC | 5 cut(s) | 83, 592, 718, 811, 1229 |
| Sfr274I | CTCGAG | 1 cut(s) | 373 |
| Sfr303I | CCGCGG | 2 cut(s) | 172, 178 |
| SgrAI | CRCCGGYG | 1 cut(s) | 568 |
| SgrBI | CCGCGG | 2 cut(s) | 172, 178 |
| SlaI | CTCGAG | 1 cut(s) | 373 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 524, 819 |
| SmlI | CTYRAG | 2 cut(s) | 373, 455 |
| SmoI | CTYRAG | 2 cut(s) | 373, 455 |
| Sse9I | AATT | 7 cut(s) | 334, 642, 912, 1005, 1186, 1236, 1565 |
| SspI | AATATT | 2 cut(s) | 832, 1078 |
| SspMI | CTAG | 3 cut(s) | 59, 713, 1628 |
| StyD4I | CCNGG | 4 cut(s) | 234, 250, 260, 444 |
| TaaI | ACNGT | 6 cut(s) | 493, 535, 613, 1096, 1547, 1656 |
| TaiI | ACGT | 1 cut(s) | 141 |
| TaqI | TCGA | 5 cut(s) | 141, 150, 290, 374, 1321 |
| TasI | AATT | 7 cut(s) | 334, 642, 912, 1005, 1186, 1236, 1565 |
| TatI | WGTACW | 2 cut(s) | 366, 480 |
| TauI | GCSGC | 9 cut(s) | 101, 171, 174, 177, 180, 183, 402, 468, 1396 |
| TfiI | GAWTC | 6 cut(s) | 242, 679, 1025, 1195, 1483, 1513 |
| Tru1I | TTAA | 5 cut(s) | 768, 1074, 1173, 1473, 1494 |
| Tru9I | TTAA | 5 cut(s) | 768, 1074, 1173, 1473, 1494 |
| TscAI | CASTG | 6 cut(s) | 388, 435, 496, 654, 1021, 1652 |
| TseFI | GTSAC | 3 cut(s) | 515, 649, 1398 |
| TseI | GCWGC | 8 cut(s) | 343, 352, 378, 595, 731, 1381, 1411, 1619 |
| Tsp45I | GTSAC | 3 cut(s) | 515, 649, 1398 |
| TspDTI | ATGAA | 5 cut(s) | 683, 859, 986, 1257, 1476 |
| TspRI | CASTG | 6 cut(s) | 388, 435, 496, 654, 1021, 1652 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 917, 1588 |
| XapI | RAATTY | 2 cut(s) | 334, 1565 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1151 |
| XhoI | CTCGAG | 1 cut(s) | 373 |
| XmnI | GAANNNNTTC | 1 cut(s) | 311 |
| XspI | CTAG | 3 cut(s) | 59, 713, 1628 |
| ZrmI | AGTACT | 1 cut(s) | 482 |