Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Forward (+)

79296092 .. 79299667

Gene Structure 3576 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G051610.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGGAGAAACCAAAAGAAAGCGAGGACGAGCTCCAAAACGCAAAACCCCAGAAACCGCCACCACCTCCTCAATGGACATCGACGACGCCGCTCCGCCCCCCACGACGGAGCCTCTCTCCGACGCCGGCACCTCCCAGCCCTCCCGCCGCGGCCGCGGCCGCCCCAGGAAGGCCGAGTCGGCTAACATCACCGCCTCGCCCGAGCGGCGGAGCTCCAGACACGCGTCGGAGCAGAACGGTGACGCCGGGGCGGTGAGTTCGGAGCCGCCGCTGATGGTGCCGAGCTGGGAGAGCGTGGCGCGCGTGGTGCCGGCGATGGACTCGGTGGTGAAGGTGTTTTGCGTCCACACGGAGCCGAATTTCTCGTTGCCGTGGCAGAGGAAGAGGCAGTACAGCTCGAGTAGTAGTGGGTTTGTGATTGGGGGAAGAAGGGTCTTGACCAATGCGCACTCTGTGGAGCACCATACACAGGTCAAGCTCAAGAAGCGTGGCTCCGAGACCAAGTACTTGGCTACCGTGTTGGCCATTGGGACTGAATGTGATATTGCCATGCTCACAGTGAGTGATGATGAGTTCTGGGAAGGAGTTTCACCTGTGGAGTTTGGAGAGTTGCCGGCGCTCCAAGATGCTGTGACGGTTGTGGGCTACCCTATTGGGGGTGACACAATCTCTGTGACGAGTGGTGTAGTGTCACGTATGGAGATACTTTCATATGTTCATGGATCAACTGAGCTTCTGGGACTGCAGATAGATGCTGCCATAAACTCTGGAAACTCTGGTGGGCCTGCCTTCAACGACAAGGGGACCTGTGTGGGCATTGCTTTTCAGTCTCTTAAACATGAAGATGCAGAAAATATAGGCTACGTTATTCCAACACCTGTTATTAAGCACTTCATTACAGATTACGAGAAGAATGGGGCATATACAGGGTTCCCAATTATTGGAATTGAGTGGCAAAAGATGGAAAATCCTGATCTACGCACATCAATGGGGATGAAAGCTGATCAAAAGGGTGTACGAATTAGAAGGATCGAGCCTACAGCTCCAGATTCTCATCTTCTGCAACCATCTGATGTCATTCTTAGCTTTGATGGAGTTAATATTGCAAATGATGGCACAGTTCCATTCAGGCATGGGGAGCGCATAGGTTTCAGTTACCTCGTCTCTCAGAAATATACTGGCGATAGTGCTGTAGTAAAAGTTCTTCGTAACTCACAGATACTTGAATTCAACATAAAACTTGGAACACACAAGCGTCTGATCCCAGCACACATCAACGGCAAGCCTCCTTCCTACTATATCATCGGTGGATTTGTTTTTTCAGCTGTGTCCGTTCCATATCTGCGTTCTGAGTATGGAAAGGATTATGACTTTGATGCTCCAGTCAAACTTTTGGACAAACATTTACATGCAATGGCAGAATCTGCTGATGAACAGCTTGTTGTCGTTTCTCAGGTACTTGTTGCCGACATTAATATTGGATACGAGGACATTGTTAACACTCAGGTTCTTGCTTTCAATGGTAAGCCTGTGAAAAATTTAAAAAACTTGGCCACCATGGTGGAGAGTTGCGATGATGAGTACCTAAAGTTTGATCTAGAATACAATCAGATGGTTGCCCTACAGACAAAAACAGCCAAAGCTGCAACTTTAGATATCCTTCTGACTCATTGCATACCTTCAGCCATGTCGGATGACCTTAAGTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

569

Amino Acids

62.08

Weight (kDa)

6.13

Isoelectric Point (pI)

50.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	121 - 299	1.1e-19	Trypsin
Trypsin_2	PF13365	136 - 273	4.4e-20	Trypsin-like peptidase domain
PDZ_2	PF13180	314 - 414	8.3e-06	PDZ domain
PDZ_3	PF17815	421 - 566	1.3e-47	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	447
AccB1I	GGYRCC	3 cut(s)	128, 277, 307
AccB7I	CCANNNNNTGG	1 cut(s)	941
AccBSI	CCGCTC	2 cut(s)	92, 205
AccII	CGCG	5 cut(s)	150, 156, 224, 301, 303
AclWI	GGATC	3 cut(s)	730, 1037, 1255
AcoI	YGGCCR	4 cut(s)	151, 157, 522, 1551
AcsI	RAATTY	3 cut(s)	358, 1226, 1537
AcuI	CTGAAG	1 cut(s)	1665
AcyI	GRCGYC	3 cut(s)	87, 123, 243
AdeI	CACNNNGTG	1 cut(s)	454
AfaI	GTAC	5 cut(s)	392, 506, 1017, 1458, 1583
AfiI	CCNNNNNNNGG	7 cut(s)	106, 169, 207, 469, 655, 656, 941
AflII	CTTAAG	1 cut(s)	1700
AflIII	ACRYGT	1 cut(s)	222
AgsI	TTSAA	4 cut(s)	793, 1226, 1231, 1519
AhdI	GACNNNNNGTC	1 cut(s)	1702
AjnI	CCWGG	1 cut(s)	164
AleI	CACNNNNGTG	1 cut(s)	1559
Alw21I	GWGCWC	3 cut(s)	34, 215, 462
Alw26I	GTCTC	3 cut(s)	491, 834, 1168
AlwI	GGATC	3 cut(s)	730, 1037, 1255
AlwNI	CAGNNNCTG	1 cut(s)	1424
Ama87I	CYCGRG	2 cut(s)	200, 397
AoxI	GGCC	6 cut(s)	151, 157, 171, 522, 782, 1551
ApeKI	GCWGC	2 cut(s)	755, 1643
ApoI	RAATTY	3 cut(s)	358, 1226, 1537
AseI	ATTAAT	1 cut(s)	1473
AspLEI	GCGC	5 cut(s)	301, 303, 448, 619, 1143
AspS9I	GGNCC	2 cut(s)	782, 804
AsuC2I	CCSGG	1 cut(s)	247
AsuHPI	GGTGA	6 cut(s)	181, 251, 265, 340, 582, 671
AvaI	CYCGRG	2 cut(s)	200, 397
AvaII	GGWCC	1 cut(s)	804
BalI	TGGCCA	2 cut(s)	524, 1553
BanI	GGYRCC	3 cut(s)	128, 277, 307
BanII	GRGCYC	2 cut(s)	34, 215
BarI	GAAGNNNNNNTAC	4 cut(s)	374, 406, 1188, 1220
Bbv12I	GWGCWC	3 cut(s)	34, 215, 462
BbvI	GCAGC	2 cut(s)	742, 1630
BccI	CCATC	7 cut(s)	268, 310, 955, 1075, 1085, 1106, 1606
BceAI	ACGGC	2 cut(s)	355, 1294
BciT130I	CCWGG	1 cut(s)	166
BciVI	GTATCC	1 cut(s)	1475
BclI	TGATCA	1 cut(s)	1003
BcnI	CCSGG	1 cut(s)	247
BcoDI	GTCTC	3 cut(s)	491, 834, 1168
BfaI	CTAG	1 cut(s)	1598
BfmI	CTRYAG	4 cut(s)	743, 1038, 1191, 1622
BfoI	RGCGCY	1 cut(s)	620
BfrI	CTTAAG	1 cut(s)	1700
BfuI	GTATCC	1 cut(s)	1475
BglI	GCCNNNNNGGC	2 cut(s)	179, 205
BmcAI	AGTACT	1 cut(s)	506
Bme1390I	CCNGG	2 cut(s)	166, 247
Bme18I	GGWCC	1 cut(s)	804
BmeRI	GACNNNNNGTC	1 cut(s)	1702
BmeT110I	CYCGRG	2 cut(s)	200, 397
BmgT120I	GGNCC	2 cut(s)	782, 804
BmiI	GGNNCC	9 cut(s)	111, 130, 264, 279, 309, 354, 493, 805, 932
BmrFI	CCNGG	2 cut(s)	166, 247
BmsI	GCATC	4 cut(s)	616, 742, 835, 1366
BoxI	GACNNNNGTC	1 cut(s)	223
BplI	GAGNNNNNCTC	2 cut(s)	101, 133
BpmI	CTGGAG	3 cut(s)	199, 1029, 1365
BpuEI	CTTGAG	1 cut(s)	464
BpuMI	CCSGG	1 cut(s)	247
BsaAI	YACGTR	1 cut(s)	695
BsaBI	GATNNNNATC	1 cut(s)	1053
BsaHI	GRCGYC	3 cut(s)	87, 123, 243
BsaI	GGTCTC	1 cut(s)	491
BsaJI	CCNNGG	6 cut(s)	148, 154, 164, 246, 371, 1557
Bsc4I	CCNNNNNNNGG	7 cut(s)	106, 169, 207, 469, 655, 656, 941
Bse118I	RCCGGY	3 cut(s)	125, 310, 613
Bse1I	ACTGG	2 cut(s)	1183, 1382
Bse3DI	GCAATG	3 cut(s)	816, 1419, 1669
Bse8I	GATNNNNATC	1 cut(s)	1053
BseBI	CCWGG	1 cut(s)	166
BseDI	CCNNGG	6 cut(s)	148, 154, 164, 246, 371, 1557
BseGI	GGATG	2 cut(s)	999, 1699
BseJI	GATNNNNATC	1 cut(s)	1053
BseLI	CCNNNNNNNGG	7 cut(s)	106, 169, 207, 469, 655, 656, 941
BseMI	GCAATG	3 cut(s)	816, 1419, 1669
BseMII	CTCAG	5 cut(s)	720, 1181, 1341, 1466, 1517
BseNI	ACTGG	2 cut(s)	1183, 1382
BsePI	GCGCGC	1 cut(s)	299
BseRI	GAGGAG	1 cut(s)	58
BseX3I	CGGCCG	2 cut(s)	151, 157
BseXI	GCAGC	2 cut(s)	742, 1630
BseYI	CCCAGC	3 cut(s)	135, 285, 1264
Bsh1236I	CGCG	5 cut(s)	150, 156, 224, 301, 303
Bsh1285I	CGRYCG	2 cut(s)	154, 160
BshFI	GGCC	6 cut(s)	153, 159, 173, 524, 784, 1553
BshNI	GGYRCC	3 cut(s)	128, 277, 307
BsiEI	CGRYCG	2 cut(s)	154, 160
BsiHKAI	GWGCWC	3 cut(s)	34, 215, 462
BsiHKCI	CYCGRG	2 cut(s)	200, 397
BsiSI	CCGG	4 cut(s)	126, 246, 311, 614
BslFI	GGGAC	3 cut(s)	544, 753, 817
BslI	CCNNNNNNNGG	7 cut(s)	106, 169, 207, 469, 655, 656, 941
BsmAI	GTCTC	3 cut(s)	491, 834, 1168
BsmBI	CGTCTC	1 cut(s)	1168
BsmFI	GGGAC	3 cut(s)	544, 753, 817
BsnI	GGCC	6 cut(s)	153, 159, 173, 524, 784, 1553
Bso31I	GGTCTC	1 cut(s)	491
BsoBI	CYCGRG	2 cut(s)	200, 397
Bsp1286I	GDGCHC	3 cut(s)	34, 215, 462
Bsp143I	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
Bsp19I	CCATGG	1 cut(s)	1557
BspANI	GGCC	6 cut(s)	153, 159, 173, 524, 784, 1553
BspCNI	CTCAG	5 cut(s)	721, 1180, 1342, 1465, 1516
BspFNI	CGCG	5 cut(s)	150, 156, 224, 301, 303
BspLI	GGNNCC	9 cut(s)	111, 130, 264, 279, 309, 354, 493, 805, 932
BspMAI	CTGCAG	1 cut(s)	747
BspPI	GGATC	3 cut(s)	730, 1037, 1255
BspT107I	GGYRCC	3 cut(s)	128, 277, 307
BspTI	CTTAAG	1 cut(s)	1700
BspTNI	GGTCTC	1 cut(s)	491
BsrBI	CCGCTC	2 cut(s)	92, 205
BsrDI	GCAATG	3 cut(s)	816, 1419, 1669
BsrFI	RCCGGY	3 cut(s)	125, 310, 613
BsrI	ACTGG	2 cut(s)	1183, 1382
BssAI	RCCGGY	3 cut(s)	125, 310, 613
BssECI	CCNNGG	6 cut(s)	148, 154, 164, 246, 371, 1557
BssHII	GCGCGC	1 cut(s)	299
BssMI	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
BssNI	GRCGYC	3 cut(s)	87, 123, 243
BssT1I	CCWWGG	1 cut(s)	1557
Bst2UI	CCWGG	1 cut(s)	166
Bst4CI	ACNGT	5 cut(s)	239, 517, 559, 637, 1120
Bst6I	CTCTTC	1 cut(s)	377
BstACI	GRCGYC	3 cut(s)	87, 123, 243
BstAFI	CTTAAG	1 cut(s)	1700
BstAPI	GCANNNNNTGC	1 cut(s)	1424
BstBAI	YACGTR	1 cut(s)	695
BstC8I	GCNNGC	6 cut(s)	127, 301, 312, 615, 786, 1283
BstDEI	CTNAG	6 cut(s)	729, 1082, 1167, 1350, 1452, 1503
BstDSI	CCRYGG	4 cut(s)	148, 154, 371, 1557
BstF5I	GGATG	2 cut(s)	999, 1699
BstFNI	CGCG	5 cut(s)	150, 156, 224, 301, 303
BstH2I	RGCGCY	1 cut(s)	620
BstHHI	GCGC	5 cut(s)	301, 303, 448, 619, 1143
BstKTI	GATC	6 cut(s)	725, 976, 1006, 1032, 1263, 1597
BstMAI	GTCTC	3 cut(s)	491, 834, 1168
BstMBI	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
BstMCI	CGRYCG	2 cut(s)	154, 160
BstNI	CCWGG	1 cut(s)	166
BstNSI	RCATGY	1 cut(s)	1412
BstPAI	GACNNNNGTC	1 cut(s)	223
BstSCI	CCNGG	2 cut(s)	164, 245
BstSFI	CTRYAG	4 cut(s)	743, 1038, 1191, 1622
BstUI	CGCG	5 cut(s)	150, 156, 224, 301, 303
BstV1I	GCAGC	2 cut(s)	742, 1630
BstXI	CCANNNNNNTGG	2 cut(s)	508, 1561
BstZI	CGGCCG	2 cut(s)	151, 157
BsuI	GTATCC	1 cut(s)	1475
BsuRI	GGCC	6 cut(s)	153, 159, 173, 524, 784, 1553
BtgI	CCRYGG	4 cut(s)	148, 154, 371, 1557
BtgZI	GCGATG	2 cut(s)	329, 1587
BtsCI	GGATG	2 cut(s)	999, 1699
BtsIMutI	CAGTG	1 cut(s)	564
Cac8I	GCNNGC	6 cut(s)	127, 301, 312, 615, 786, 1283
CaiI	CAGNNNCTG	1 cut(s)	1424
CciNI	GCGGCCGC	2 cut(s)	151, 157
CfoI	GCGC	5 cut(s)	301, 303, 448, 619, 1143
Cfr10I	RCCGGY	3 cut(s)	125, 310, 613
Cfr13I	GGNCC	2 cut(s)	782, 804
Cfr42I	CCGCGG	2 cut(s)	151, 157
CseI	GACGC	6 cut(s)	95, 131, 213, 251, 331, 1244
Csp6I	GTAC	5 cut(s)	391, 505, 1016, 1457, 1582
CspCI	CAANNNNNGTGG	2 cut(s)	469, 504
CviAII	CATG	7 cut(s)	550, 719, 839, 1133, 1409, 1558, 1687
CviQI	GTAC	5 cut(s)	391, 505, 1016, 1457, 1582
DdeI	CTNAG	6 cut(s)	729, 1082, 1167, 1350, 1452, 1503
DpnI	GATC	6 cut(s)	724, 975, 1005, 1031, 1262, 1596
DpnII	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
DraI	TTTAAA	1 cut(s)	1542
DraIII	CACNNNGTG	1 cut(s)	454
DriI	GACNNNNNGTC	1 cut(s)	1702
EaeI	YGGCCR	4 cut(s)	151, 157, 522, 1551
EagI	CGGCCG	2 cut(s)	151, 157
Eam1104I	CTCTTC	1 cut(s)	377
Eam1105I	GACNNNNNGTC	1 cut(s)	1702
EarI	CTCTTC	1 cut(s)	377
EciI	GGCGGA	2 cut(s)	84, 223
Ecl136II	GAGCTC	2 cut(s)	32, 213
EclXI	CGGCCG	2 cut(s)	151, 157
Eco130I	CCWWGG	1 cut(s)	1557
Eco24I	GRGCYC	2 cut(s)	34, 215
Eco31I	GGTCTC	1 cut(s)	491
Eco32I	GATATC	1 cut(s)	1657
Eco47I	GGWCC	1 cut(s)	804
Eco52I	CGGCCG	2 cut(s)	151, 157
Eco53kI	GAGCTC	2 cut(s)	32, 213
Eco57I	CTGAAG	1 cut(s)	1665
Eco88I	CYCGRG	2 cut(s)	200, 397
EcoICRI	GAGCTC	2 cut(s)	32, 213
EcoO109I	RGGNCCY	1 cut(s)	804
EcoRI	GAATTC	1 cut(s)	1226
EcoRII	CCWGG	1 cut(s)	164
EcoRV	GATATC	1 cut(s)	1657
EcoT14I	CCWWGG	1 cut(s)	1557
EcoT38I	GRGCYC	2 cut(s)	34, 215
ErhI	CCWWGG	1 cut(s)	1557
Esp3I	CGTCTC	1 cut(s)	1168
FaeI	CATG	7 cut(s)	553, 722, 842, 1136, 1412, 1561, 1690
FaqI	GGGAC	3 cut(s)	544, 753, 817
FatI	CATG	7 cut(s)	549, 718, 838, 1132, 1408, 1557, 1686
FauI	CCCGC	1 cut(s)	152
FauNDI	CATATG	1 cut(s)	712
FbaI	TGATCA	1 cut(s)	1003
FokI	GGATG	2 cut(s)	1006, 1706
FriOI	GRGCYC	2 cut(s)	34, 215
FspAI	RTGCGCAY	1 cut(s)	447
FspBI	CTAG	1 cut(s)	1598
FspI	TGCGCA	1 cut(s)	447
GlaI	GCGC	5 cut(s)	300, 302, 447, 618, 1142
GsaI	CCCAGC	3 cut(s)	139, 289, 1268
GsuI	CTGGAG	3 cut(s)	199, 1029, 1365
HaeII	RGCGCY	1 cut(s)	620
HaeIII	GGCC	6 cut(s)	153, 159, 173, 524, 784, 1553
HapII	CCGG	4 cut(s)	126, 246, 311, 614
HgaI	GACGC	6 cut(s)	95, 131, 213, 251, 331, 1244
HhaI	GCGC	5 cut(s)	301, 303, 448, 619, 1143
Hin1I	GRCGYC	3 cut(s)	87, 123, 243
Hin1II	CATG	7 cut(s)	553, 722, 842, 1136, 1412, 1561, 1690
Hin6I	GCGC	5 cut(s)	299, 301, 446, 617, 1141
HinP1I	GCGC	5 cut(s)	299, 301, 446, 617, 1141
HincII	GTYRAC	1 cut(s)	1498
HindII	GTYRAC	1 cut(s)	1498
HinfI	GANTC	5 cut(s)	176, 320, 1049, 1421, 1666
HpaI	GTTAAC	1 cut(s)	1498
HpaII	CCGG	4 cut(s)	126, 246, 311, 614
HphI	GGTGA	6 cut(s)	181, 251, 265, 340, 582, 671
Hpy166II	GTNNAC	3 cut(s)	346, 1016, 1498
Hpy188III	TCNNGA	7 cut(s)	216, 436, 481, 768, 971, 1046, 1598
Hpy8I	GTNNAC	3 cut(s)	346, 1016, 1498
Hpy99I	CGWCG	5 cut(s)	86, 89, 109, 125, 229
HpyAV	CCTTC	9 cut(s)	163, 325, 423, 575, 799, 1020, 1299, 1670, 1689
HpyCH4III	ACNGT	5 cut(s)	239, 517, 559, 637, 1120
HpyCH4IV	ACGT	2 cut(s)	694, 864
HpyCH4V	TGCA	7 cut(s)	745, 848, 1063, 1106, 1412, 1646, 1674
HpyF3I	CTNAG	6 cut(s)	729, 1082, 1167, 1350, 1452, 1503
HpySE526I	ACGT	2 cut(s)	694, 864
Hsp92I	GRCGYC	3 cut(s)	87, 123, 243
Hsp92II	CATG	7 cut(s)	553, 722, 842, 1136, 1412, 1561, 1690
HspAI	GCGC	5 cut(s)	299, 301, 446, 617, 1141
KroI	GCCGGC	3 cut(s)	125, 310, 613
KroNI	GCCGGC	3 cut(s)	127, 312, 615
Ksp22I	TGATCA	1 cut(s)	1003
KspAI	GTTAAC	1 cut(s)	1498
KspI	CCGCGG	2 cut(s)	151, 157
Kzo9I	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
Lsp1109I	GCAGC	2 cut(s)	742, 1630
LweI	GCATC	4 cut(s)	616, 742, 835, 1366
MaeI	CTAG	1 cut(s)	1598
MaeII	ACGT	2 cut(s)	694, 864
MaeIII	GTNAC	7 cut(s)	239, 631, 659, 673, 690, 1154, 1208
MalI	GATC	6 cut(s)	724, 975, 1005, 1031, 1262, 1596
MbiI	CCGCTC	2 cut(s)	92, 205
MboI	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
MboII	GAAGA	6 cut(s)	394, 438, 854, 922, 1049, 1196
MhlI	GDGCHC	3 cut(s)	34, 215, 462
MlsI	TGGCCA	2 cut(s)	524, 1553
MluCI	AATT	6 cut(s)	358, 936, 945, 1020, 1226, 1537
MluI	ACGCGT	1 cut(s)	222
MluNI	TGGCCA	2 cut(s)	524, 1553
MlyI	GAGTC	3 cut(s)	185, 314, 1660
MmeI	TCCRAC	4 cut(s)	144, 207, 896, 1671
Mox20I	TGGCCA	2 cut(s)	524, 1553
MroNI	GCCGGC	3 cut(s)	125, 310, 613
MscI	TGGCCA	2 cut(s)	524, 1553
MseI	TTAA	8 cut(s)	834, 885, 1098, 1473, 1497, 1541, 1701, 1708
MslI	CAYNNNNRTG	4 cut(s)	843, 986, 1407, 1559
Msp20I	TGGCCA	2 cut(s)	524, 1553
MspA1I	CMGCKG	4 cut(s)	150, 156, 271, 1325
MspCI	CTTAAG	1 cut(s)	1700
MspI	CCGG	4 cut(s)	126, 246, 311, 614
MspR9I	CCNGG	2 cut(s)	166, 247
MvaI	CCWGG	1 cut(s)	166
MvnI	CGCG	5 cut(s)	150, 156, 224, 301, 303
NaeI	GCCGGC	3 cut(s)	127, 312, 615
NciI	CCSGG	1 cut(s)	247
NcoI	CCATGG	1 cut(s)	1557
NdeI	CATATG	1 cut(s)	712
NdeII	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
NgoMIV	GCCGGC	3 cut(s)	125, 310, 613
NlaIII	CATG	7 cut(s)	553, 722, 842, 1136, 1412, 1561, 1690
NlaIV	GGNNCC	9 cut(s)	111, 130, 264, 279, 309, 354, 493, 805, 932
NmeAIII	GCCGAG	2 cut(s)	199, 306
NmuCI	GTSAC	5 cut(s)	239, 631, 659, 673, 690
NotI	GCGGCCGC	2 cut(s)	151, 157
NsbI	TGCGCA	1 cut(s)	447
NspI	RCATGY	1 cut(s)	1412
OliI	CACNNNNGTG	1 cut(s)	1559
PaeR7I	CTCGAG	1 cut(s)	397
PauI	GCGCGC	1 cut(s)	299
PdiI	GCCGGC	3 cut(s)	127, 312, 615
PfeI	GAWTC	2 cut(s)	1049, 1421
PflMI	CCANNNNNTGG	1 cut(s)	941
PleI	GAGTC	3 cut(s)	184, 314, 1660
PpsI	GAGTC	3 cut(s)	184, 314, 1660
Ppu21I	YACGTR	1 cut(s)	695
PpuMI	RGGWCCY	1 cut(s)	804
PshAI	GACNNNNGTC	1 cut(s)	223
PshBI	ATTAAT	1 cut(s)	1473
Psp124BI	GAGCTC	2 cut(s)	34, 215
Psp5II	RGGWCCY	1 cut(s)	804
Psp6I	CCWGG	1 cut(s)	164
PspFI	CCCAGC	3 cut(s)	135, 285, 1264
PspGI	CCWGG	1 cut(s)	164
PspN4I	GGNNCC	9 cut(s)	111, 130, 264, 279, 309, 354, 493, 805, 932
PspPI	GGNCC	2 cut(s)	782, 804
PspPPI	RGGWCCY	1 cut(s)	804
PspXI	VCTCGAGB	1 cut(s)	397
PsrI	GAACNNNNNNTAC	2 cut(s)	1185, 1217
PstI	CTGCAG	1 cut(s)	747
PstNI	CAGNNNCTG	1 cut(s)	1424
PteI	GCGCGC	1 cut(s)	299
PvuII	CAGCTG	1 cut(s)	1325
RsaI	GTAC	5 cut(s)	392, 506, 1017, 1458, 1583
RsaNI	GTAC	5 cut(s)	391, 505, 1016, 1457, 1582
RseI	CAYNNNNRTG	4 cut(s)	843, 986, 1407, 1559
SacI	GAGCTC	2 cut(s)	34, 215
SacII	CCGCGG	2 cut(s)	151, 157
SaqAI	TTAA	8 cut(s)	834, 885, 1098, 1473, 1497, 1541, 1701, 1708
Sau3AI	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
Sau96I	GGNCC	2 cut(s)	782, 804
ScaI	AGTACT	1 cut(s)	506
SchI	GAGTC	3 cut(s)	185, 314, 1660
ScrFI	CCNGG	2 cut(s)	166, 247
SduI	GDGCHC	3 cut(s)	34, 215, 462
SfaNI	GCATC	4 cut(s)	616, 742, 835, 1366
SfcI	CTRYAG	4 cut(s)	743, 1038, 1191, 1622
Sfr274I	CTCGAG	1 cut(s)	397
Sfr303I	CCGCGG	2 cut(s)	151, 157
SgrBI	CCGCGG	2 cut(s)	151, 157
SinI	GGWCC	1 cut(s)	804
SlaI	CTCGAG	1 cut(s)	397
SmiMI	CAYNNNNRTG	4 cut(s)	843, 986, 1407, 1559
SmlI	CTYRAG	3 cut(s)	397, 479, 1700
SmoI	CTYRAG	3 cut(s)	397, 479, 1700
Sse9I	AATT	6 cut(s)	358, 936, 945, 1020, 1226, 1537
SspI	AATATT	2 cut(s)	1102, 1477
SspMI	CTAG	1 cut(s)	1598
SstI	GAGCTC	2 cut(s)	34, 215
StyD4I	CCNGG	2 cut(s)	164, 245
StyI	CCWWGG	1 cut(s)	1557
TaaI	ACNGT	5 cut(s)	239, 517, 559, 637, 1120
TaiI	ACGT	2 cut(s)	697, 867
TaqI	TCGA	3 cut(s)	81, 398, 1032
TasI	AATT	6 cut(s)	358, 936, 945, 1020, 1226, 1537
TatI	WGTACW	2 cut(s)	390, 504
TauI	GCSGC	9 cut(s)	92, 150, 153, 156, 159, 162, 208, 268, 271
TfiI	GAWTC	2 cut(s)	1049, 1421
Tru1I	TTAA	8 cut(s)	834, 885, 1098, 1473, 1497, 1541, 1701, 1708
Tru9I	TTAA	8 cut(s)	834, 885, 1098, 1473, 1497, 1541, 1701, 1708
TscAI	CASTG	1 cut(s)	564
TseFI	GTSAC	5 cut(s)	239, 631, 659, 673, 690
TseI	GCWGC	2 cut(s)	755, 1643
Tsp45I	GTSAC	5 cut(s)	239, 631, 659, 673, 690
TspDTI	ATGAA	6 cut(s)	699, 707, 855, 883, 1010, 1446
TspGWI	ACGGA	3 cut(s)	122, 365, 1321
TspRI	CASTG	1 cut(s)	564
Van91I	CCANNNNNTGG	1 cut(s)	941
Vha464I	CTTAAG	1 cut(s)	1700
VpaK11BI	GGWCC	1 cut(s)	804
VspI	ATTAAT	1 cut(s)	1473
XapI	RAATTY	3 cut(s)	358, 1226, 1537
XbaI	TCTAGA	1 cut(s)	1597
XceI	RCATGY	1 cut(s)	1412
XcmI	CCANNNNNNNNNTGG	1 cut(s)	70
XhoI	CTCGAG	1 cut(s)	397
XspI	CTAG	1 cut(s)	1598
ZrmI	AGTACT	1 cut(s)	506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.