Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001244

Physical Location & Seq

Genomic Coordinates Forward (+)

79666284 .. 79669894

Gene Structure 3611 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021973

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGGGAGAAACCAAGAGAAAGCGAGGGAGAGCTCCAAAACCCAAAACCCCAGAAACCGCCACCACCTCAATGGACGTCGACGACGCCGCTCCGCCCCCCACGACGGAGCCTCTCTCCGACGCCGGCACCTCCCAGCCCTCCCGCCGCGGCCGCGGCCGCCCCAGGAAGGCCGAGTCGGCCAACATCACCGCCTCGCCGGAGCGGCGGAGCTCCCGGCACGCGTCGGAGCAGAACGGCGACGCCGGGGCGGTGAGTTCGGAGCCGCCGCTGATGGTGCCGAGCTGGGAGAGCGTGGCGCGCGTGGTGCCGGCGATGGACTCGGTGGTGAAGGTGTTTTGCGTCCACACGGAGCCGAATTTCTCGTTGCCGTGGCAGAGGAAGAGGCAGTACAGCTCGAGTAGCAGTGGGTTTGTGATTGGAGGAAGAAGGGTCTTGACCAATGCGCACTCTGTGGAGCACCATACACAGGTCAAGCTCAAGAAGCGTGGCTCCGAGACCAAGTACTTGGCTACTGTGTTGGCGATTGGGACTGAATGTGATATTGCCATGCTCACGGTGAGTGATGATGAGTTCTGGGAAGGAGTTTCACCTGTGGAGTTTGGAGAGTTGCCGGCACTCCAAGACGCTGTGACGGTTGTGGGCTACCCTATTGGGGGTGACACAATCTCTGTGACGAGTGGTGTTGTGTCACGTATGGAGATACTGTCATATGTTCATGGATCAACTGAGCTTCTCGGACTGCAGATAGATGCTGCCATAAACTCTGGAAACTCTGGTGGGCCTGCCTTCAACGACAAGGGGACCTGTGTGGGCATTGCTTTTCAGTCCCTTAAACATGAAGATGCAGAAAATATAGGCTACGTTATTCCAACACCTGTTATTAAGCACTTCATTACAGATTACGAGAAGAATGGGGCATATACAGGGTTCCCAATTATTGGAATTGAGTGGCAAAAGATGGAAAATCCTGATCTACGAACGTCAATGGGGATGAAAGCTGATCAAAAGGGTGTACGTATTAGAAGGATCGAACCTACAGCTCCAGATGCTCATCTTCTGCAACCATCTGATGTCATTCTCAGCTTTGATGGAGTTAATATTGCAAACGATGGCACAGTTCCATTCAGGCATGGGGAGCGCATAGGTTTCAGTTACCTCGTCTCTCAGAAATATACTGGCGATAGTGCTGTAGTAAAAGTTCTTCGTAACTCACAGATACTTGAATTCAACATAAAACTTGGAACACACAAGCGTCTGATCCCAGCACACATCAATGGCAAACCACCTTCCTATTATATCATCGGTGGATTTGTTTTTTCAGCTGTATCTGTTCCATATCTGCGTTCTGAGTATGGAAAGGATTATGACTTTGATGCTCCAGTCAAACTTTTGGACAAGCATTTACATGCAATGGCAGAATCTGCTGATGAACAGCTTGTTGTCGTTTCTCAGGTACTTGTTGCTGACATTAATATCGGATATGAGGACATTGTTAACACTCAGGTTCTTGCTTTTAATGGTAAGCCTGTGAAAAATTTAAAAAACTTGGCCACCATGGTGGAGAGTTGCGATGATGAGTACCTAAAGTTTGATCTAGAATACAATCAGATGGTTGCCCTACAGACAAAAACAGCCAAAGCTGCAACTTTAGATATCCTTCTGACTCATTGCATACCTTCAGCCATGTCTGATGACCTTAAGACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0009507 GO:0009536 GO:0016787 GO:0017171 GO:0019538 GO:0031974 GO:0031981 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0070011 GO:0070013 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

569

Amino Acids

61.92

Weight (kDa)

6.05

Isoelectric Point (pI)

48.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	120 - 298	1.1e-19	Trypsin
Trypsin_2	PF13365	135 - 272	4.4e-20	Trypsin-like peptidase domain
PDZ_2	PF13180	313 - 413	4.8e-06	PDZ domain
PDZ_3	PF17815	420 - 565	1.3e-47	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001244)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40200
fragaria_vesca	FvH4_5g04990 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350 FvH4_6g50350
malus_domestica	MD06G1178100.v1.1 MD06G1178200.v1.1 MD09G1037000.v1.1 MD09G1037100.v1.1 MD14G1184600.v1.1 MD17G1038400.v1.1 MD17G1038500.v1.1
prunus_persica	Prupe.3G280500_v2.0.a1 Prupe.3G280600_v2.0.a1 Prupe.5G177300_v2.0.a1 Prupe.5G177300_v2.0.a1
pyrus_communis	pycom06g15840 pycom111g02980 pycom14g15360 pycom17g03470
rosa_chinensis	RchiOBHm_Chr1g0327041 RchiOBHm_Chr2g0170661 RchiOBHm_Chr2g0170671 RchiOBHm_Chr7g0194051
rosa_laevigata	RLG00000004221 RLG00000021973 RLG00000021974
rosa_multiflora	Rmu_sc0003787.1_g000010 Rmu_sc0003787.1_g000011 Rmu_sc0009433.1_g000001 Rmu_sc0023958.1_g000001 Rmu_sc0042111.1_g000001
rosa_roxburghii	Rroxscaffold_2G00081130 Rroxscaffold_2G00081140 Rroxscaffold_3G00261590
rosa_rugosa	Rorug02G0550300 Rorug07G0013500
rosa_samantha	Rh2AG623200 Rh2AG623300 Rh2BG633700 Rh2CG602900 Rh2CG603000 Rh2DG646500 Rh7AG140600 Rh7BG141300 Rh7CG144700 Rh7DG142000 Rh7DG324300
rosa_wichuraiana	Rw2G028950 Rw2G051610 Rw2G051620 Rw7G012010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	78
Acc16I	TGCGCA	1 cut(s)	444
AccB1I	GGYRCC	3 cut(s)	125, 274, 304
AccB7I	CCANNNNNTGG	1 cut(s)	938
AccBSI	CCGCTC	2 cut(s)	89, 202
AccI	GTMKAC	1 cut(s)	78
AccII	CGCG	5 cut(s)	147, 153, 221, 298, 300
AclWI	GGATC	3 cut(s)	727, 1034, 1252
AcoI	YGGCCR	4 cut(s)	148, 154, 177, 1548
AcsI	RAATTY	3 cut(s)	355, 1223, 1534
AcuI	CTGAAG	1 cut(s)	1662
AcyI	GRCGYC	4 cut(s)	75, 84, 120, 240
AdeI	CACNNNGTG	1 cut(s)	451
AfaI	GTAC	5 cut(s)	389, 503, 1014, 1455, 1580
AfiI	CCNNNNNNNGG	6 cut(s)	103, 166, 466, 652, 653, 938
AflII	CTTAAG	1 cut(s)	1697
AflIII	ACRYGT	1 cut(s)	219
AgsI	TTSAA	3 cut(s)	790, 1223, 1228
AjnI	CCWGG	1 cut(s)	161
AleI	CACNNNNGTG	1 cut(s)	1556
Alw21I	GWGCWC	3 cut(s)	34, 212, 459
Alw26I	GTCTC	2 cut(s)	488, 1165
AlwI	GGATC	3 cut(s)	727, 1034, 1252
AlwNI	CAGNNNCTG	1 cut(s)	1421
Ama87I	CYCGRG	1 cut(s)	394
AoxI	GGCC	6 cut(s)	148, 154, 168, 177, 779, 1548
ApeKI	GCWGC	2 cut(s)	752, 1640
ApoI	RAATTY	3 cut(s)	355, 1223, 1534
AseI	ATTAAT	1 cut(s)	1470
AspLEI	GCGC	4 cut(s)	298, 300, 445, 1140
AspS9I	GGNCC	2 cut(s)	779, 801
AsuC2I	CCSGG	2 cut(s)	214, 244
AsuHPI	GGTGA	6 cut(s)	178, 262, 337, 568, 579, 668
AvaI	CYCGRG	1 cut(s)	394
AvaII	GGWCC	1 cut(s)	801
BalI	TGGCCA	1 cut(s)	1550
BanI	GGYRCC	3 cut(s)	125, 274, 304
BanII	GRGCYC	2 cut(s)	34, 212
BarI	GAAGNNNNNNTAC	4 cut(s)	371, 403, 1185, 1217
Bbv12I	GWGCWC	3 cut(s)	34, 212, 459
BbvI	GCAGC	2 cut(s)	739, 1627
BccI	CCATC	7 cut(s)	265, 307, 952, 1072, 1082, 1103, 1603
BceAI	ACGGC	2 cut(s)	250, 352
BciT130I	CCWGG	1 cut(s)	163
BclI	TGATCA	1 cut(s)	1000
BcnI	CCSGG	2 cut(s)	214, 244
BcoDI	GTCTC	2 cut(s)	488, 1165
BfaI	CTAG	1 cut(s)	1595
BfmI	CTRYAG	4 cut(s)	740, 1035, 1188, 1619
BfrI	CTTAAG	1 cut(s)	1697
BglI	GCCNNNNNGGC	2 cut(s)	176, 202
BmcAI	AGTACT	1 cut(s)	503
Bme1390I	CCNGG	3 cut(s)	163, 214, 244
Bme18I	GGWCC	1 cut(s)	801
BmeT110I	CYCGRG	1 cut(s)	394
BmgT120I	GGNCC	2 cut(s)	779, 801
BmiI	GGNNCC	9 cut(s)	108, 127, 261, 276, 306, 351, 490, 802, 929
BmrFI	CCNGG	3 cut(s)	163, 214, 244
BmsI	GCATC	4 cut(s)	739, 832, 1036, 1363
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
BpmI	CTGGAG	2 cut(s)	1026, 1362
BpuEI	CTTGAG	1 cut(s)	461
BpuMI	CCSGG	2 cut(s)	214, 244
BsaAI	YACGTR	2 cut(s)	692, 1016
BsaBI	GATNNNNATC	1 cut(s)	1050
BsaHI	GRCGYC	4 cut(s)	75, 84, 120, 240
BsaI	GGTCTC	1 cut(s)	488
BsaJI	CCNNGG	6 cut(s)	145, 151, 161, 243, 368, 1554
BsaXI	ACNNNNNCTCC	2 cut(s)	689, 719
Bsc4I	CCNNNNNNNGG	6 cut(s)	103, 166, 466, 652, 653, 938
Bse118I	RCCGGY	3 cut(s)	122, 307, 610
Bse1I	ACTGG	2 cut(s)	1180, 1379
Bse3DI	GCAATG	3 cut(s)	813, 1416, 1666
Bse8I	GATNNNNATC	1 cut(s)	1050
BseBI	CCWGG	1 cut(s)	163
BseDI	CCNNGG	6 cut(s)	145, 151, 161, 243, 368, 1554
BseGI	GGATG	1 cut(s)	996
BseJI	GATNNNNATC	1 cut(s)	1050
BseLI	CCNNNNNNNGG	6 cut(s)	103, 166, 466, 652, 653, 938
BseMI	GCAATG	3 cut(s)	813, 1416, 1666
BseMII	CTCAG	6 cut(s)	717, 1093, 1178, 1338, 1463, 1514
BseNI	ACTGG	2 cut(s)	1180, 1379
BsePI	GCGCGC	1 cut(s)	296
BseX3I	CGGCCG	2 cut(s)	148, 154
BseXI	GCAGC	2 cut(s)	739, 1627
BseYI	CCCAGC	3 cut(s)	132, 282, 1261
Bsh1236I	CGCG	5 cut(s)	147, 153, 221, 298, 300
Bsh1285I	CGRYCG	2 cut(s)	151, 157
BshFI	GGCC	6 cut(s)	150, 156, 170, 179, 781, 1550
BshNI	GGYRCC	3 cut(s)	125, 274, 304
BsiEI	CGRYCG	2 cut(s)	151, 157
BsiHKAI	GWGCWC	3 cut(s)	34, 212, 459
BsiHKCI	CYCGRG	1 cut(s)	394
BsiSI	CCGG	6 cut(s)	123, 197, 214, 243, 308, 611
BslFI	GGGAC	3 cut(s)	541, 811, 814
BslI	CCNNNNNNNGG	6 cut(s)	103, 166, 466, 652, 653, 938
BsmAI	GTCTC	2 cut(s)	488, 1165
BsmBI	CGTCTC	1 cut(s)	1165
BsmFI	GGGAC	3 cut(s)	541, 811, 814
BsnI	GGCC	6 cut(s)	150, 156, 170, 179, 781, 1550
Bso31I	GGTCTC	1 cut(s)	488
BsoBI	CYCGRG	1 cut(s)	394
Bsp1286I	GDGCHC	3 cut(s)	34, 212, 459
Bsp143I	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
Bsp19I	CCATGG	1 cut(s)	1554
BspANI	GGCC	6 cut(s)	150, 156, 170, 179, 781, 1550
BspCNI	CTCAG	6 cut(s)	718, 1092, 1177, 1339, 1462, 1513
BspFNI	CGCG	5 cut(s)	147, 153, 221, 298, 300
BspLI	GGNNCC	9 cut(s)	108, 127, 261, 276, 306, 351, 490, 802, 929
BspMAI	CTGCAG	1 cut(s)	744
BspPI	GGATC	3 cut(s)	727, 1034, 1252
BspT107I	GGYRCC	3 cut(s)	125, 274, 304
BspTI	CTTAAG	1 cut(s)	1697
BspTNI	GGTCTC	1 cut(s)	488
BsrBI	CCGCTC	2 cut(s)	89, 202
BsrDI	GCAATG	3 cut(s)	813, 1416, 1666
BsrFI	RCCGGY	3 cut(s)	122, 307, 610
BsrI	ACTGG	2 cut(s)	1180, 1379
BssAI	RCCGGY	3 cut(s)	122, 307, 610
BssECI	CCNNGG	6 cut(s)	145, 151, 161, 243, 368, 1554
BssHII	GCGCGC	1 cut(s)	296
BssMI	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
BssNI	GRCGYC	4 cut(s)	75, 84, 120, 240
BssT1I	CCWWGG	1 cut(s)	1554
Bst2UI	CCWGG	1 cut(s)	163
Bst4CI	ACNGT	5 cut(s)	514, 556, 634, 705, 1117
Bst6I	CTCTTC	1 cut(s)	374
BstACI	GRCGYC	4 cut(s)	75, 84, 120, 240
BstAFI	CTTAAG	1 cut(s)	1697
BstAPI	GCANNNNNTGC	1 cut(s)	1421
BstBAI	YACGTR	2 cut(s)	692, 1016
BstC8I	GCNNGC	6 cut(s)	124, 219, 298, 309, 612, 783
BstDEI	CTNAG	6 cut(s)	726, 1079, 1164, 1347, 1449, 1500
BstDSI	CCRYGG	4 cut(s)	145, 151, 368, 1554
BstF5I	GGATG	1 cut(s)	996
BstFNI	CGCG	5 cut(s)	147, 153, 221, 298, 300
BstHHI	GCGC	4 cut(s)	298, 300, 445, 1140
BstKTI	GATC	6 cut(s)	722, 973, 1003, 1029, 1260, 1594
BstMAI	GTCTC	2 cut(s)	488, 1165
BstMBI	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
BstMCI	CGRYCG	2 cut(s)	151, 157
BstNI	CCWGG	1 cut(s)	163
BstNSI	RCATGY	1 cut(s)	1409
BstSCI	CCNGG	3 cut(s)	161, 212, 242
BstSFI	CTRYAG	4 cut(s)	740, 1035, 1188, 1619
BstSNI	TACGTA	1 cut(s)	1016
BstUI	CGCG	5 cut(s)	147, 153, 221, 298, 300
BstV1I	GCAGC	2 cut(s)	739, 1627
BstXI	CCANNNNNNTGG	3 cut(s)	70, 505, 1558
BstZI	CGGCCG	2 cut(s)	148, 154
BsuRI	GGCC	6 cut(s)	150, 156, 170, 179, 781, 1550
BtgI	CCRYGG	4 cut(s)	145, 151, 368, 1554
BtgZI	GCGATG	2 cut(s)	326, 1584
BtsCI	GGATG	1 cut(s)	996
BtsI	GCAGTG	1 cut(s)	409
BtsIMutI	CAGTG	1 cut(s)	409
Cac8I	GCNNGC	6 cut(s)	124, 219, 298, 309, 612, 783
CaiI	CAGNNNCTG	1 cut(s)	1421
CciNI	GCGGCCGC	2 cut(s)	148, 154
CfoI	GCGC	4 cut(s)	298, 300, 445, 1140
Cfr10I	RCCGGY	3 cut(s)	122, 307, 610
Cfr13I	GGNCC	2 cut(s)	779, 801
Cfr42I	CCGCGG	2 cut(s)	148, 154
CseI	GACGC	7 cut(s)	92, 128, 210, 248, 328, 632, 1241
Csp6I	GTAC	5 cut(s)	388, 502, 1013, 1454, 1579
CspCI	CAANNNNNGTGG	2 cut(s)	466, 501
CviAII	CATG	7 cut(s)	547, 716, 836, 1130, 1406, 1555, 1684
CviQI	GTAC	5 cut(s)	388, 502, 1013, 1454, 1579
DdeI	CTNAG	6 cut(s)	726, 1079, 1164, 1347, 1449, 1500
DpnI	GATC	6 cut(s)	721, 972, 1002, 1028, 1259, 1593
DpnII	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
DraI	TTTAAA	1 cut(s)	1539
DraIII	CACNNNGTG	1 cut(s)	451
EaeI	YGGCCR	4 cut(s)	148, 154, 177, 1548
EagI	CGGCCG	2 cut(s)	148, 154
Eam1104I	CTCTTC	1 cut(s)	374
EarI	CTCTTC	1 cut(s)	374
EciI	GGCGGA	2 cut(s)	81, 220
Ecl136II	GAGCTC	2 cut(s)	32, 210
EclXI	CGGCCG	2 cut(s)	148, 154
Eco105I	TACGTA	1 cut(s)	1016
Eco130I	CCWWGG	1 cut(s)	1554
Eco24I	GRGCYC	2 cut(s)	34, 212
Eco31I	GGTCTC	1 cut(s)	488
Eco32I	GATATC	1 cut(s)	1654
Eco47I	GGWCC	1 cut(s)	801
Eco52I	CGGCCG	2 cut(s)	148, 154
Eco53kI	GAGCTC	2 cut(s)	32, 210
Eco57I	CTGAAG	1 cut(s)	1662
Eco88I	CYCGRG	1 cut(s)	394
EcoICRI	GAGCTC	2 cut(s)	32, 210
EcoO109I	RGGNCCY	1 cut(s)	801
EcoRI	GAATTC	1 cut(s)	1223
EcoRII	CCWGG	1 cut(s)	161
EcoRV	GATATC	1 cut(s)	1654
EcoT14I	CCWWGG	1 cut(s)	1554
EcoT38I	GRGCYC	2 cut(s)	34, 212
ErhI	CCWWGG	1 cut(s)	1554
Esp3I	CGTCTC	1 cut(s)	1165
FaeI	CATG	7 cut(s)	550, 719, 839, 1133, 1409, 1558, 1687
FaqI	GGGAC	3 cut(s)	541, 811, 814
FatI	CATG	7 cut(s)	546, 715, 835, 1129, 1405, 1554, 1683
FauI	CCCGC	1 cut(s)	149
FauNDI	CATATG	1 cut(s)	709
FbaI	TGATCA	1 cut(s)	1000
FblI	GTMKAC	1 cut(s)	78
FokI	GGATG	1 cut(s)	1003
FriOI	GRGCYC	2 cut(s)	34, 212
FspAI	RTGCGCAY	1 cut(s)	444
FspBI	CTAG	1 cut(s)	1595
FspI	TGCGCA	1 cut(s)	444
GlaI	GCGC	4 cut(s)	297, 299, 444, 1139
GsaI	CCCAGC	3 cut(s)	136, 286, 1265
GsuI	CTGGAG	2 cut(s)	1026, 1362
HaeIII	GGCC	6 cut(s)	150, 156, 170, 179, 781, 1550
HapII	CCGG	6 cut(s)	123, 197, 214, 243, 308, 611
HgaI	GACGC	7 cut(s)	92, 128, 210, 248, 328, 632, 1241
HhaI	GCGC	4 cut(s)	298, 300, 445, 1140
Hin1I	GRCGYC	4 cut(s)	75, 84, 120, 240
Hin1II	CATG	7 cut(s)	550, 719, 839, 1133, 1409, 1558, 1687
Hin6I	GCGC	4 cut(s)	296, 298, 443, 1138
HinP1I	GCGC	4 cut(s)	296, 298, 443, 1138
HincII	GTYRAC	2 cut(s)	79, 1495
HindII	GTYRAC	2 cut(s)	79, 1495
HinfI	GANTC	4 cut(s)	173, 317, 1418, 1663
HpaI	GTTAAC	1 cut(s)	1495
HpaII	CCGG	6 cut(s)	123, 197, 214, 243, 308, 611
HphI	GGTGA	6 cut(s)	178, 262, 337, 568, 579, 668
Hpy166II	GTNNAC	4 cut(s)	79, 343, 1013, 1495
Hpy188III	TCNNGA	6 cut(s)	433, 478, 765, 968, 1043, 1595
Hpy8I	GTNNAC	4 cut(s)	79, 343, 1013, 1495
Hpy99I	CGWCG	7 cut(s)	80, 83, 86, 106, 122, 226, 242
HpyAV	CCTTC	9 cut(s)	160, 322, 420, 572, 796, 1017, 1296, 1667, 1686
HpyCH4III	ACNGT	5 cut(s)	514, 556, 634, 705, 1117
HpyCH4IV	ACGT	5 cut(s)	75, 691, 861, 980, 1015
HpyCH4V	TGCA	7 cut(s)	742, 845, 1060, 1103, 1409, 1643, 1671
HpyF3I	CTNAG	6 cut(s)	726, 1079, 1164, 1347, 1449, 1500
HpySE526I	ACGT	5 cut(s)	75, 691, 861, 980, 1015
Hsp92I	GRCGYC	4 cut(s)	75, 84, 120, 240
Hsp92II	CATG	7 cut(s)	550, 719, 839, 1133, 1409, 1558, 1687
HspAI	GCGC	4 cut(s)	296, 298, 443, 1138
KroI	GCCGGC	3 cut(s)	122, 307, 610
KroNI	GCCGGC	3 cut(s)	124, 309, 612
Ksp22I	TGATCA	1 cut(s)	1000
KspAI	GTTAAC	1 cut(s)	1495
KspI	CCGCGG	2 cut(s)	148, 154
Kzo9I	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
Lsp1109I	GCAGC	2 cut(s)	739, 1627
LweI	GCATC	4 cut(s)	739, 832, 1036, 1363
MaeI	CTAG	1 cut(s)	1595
MaeII	ACGT	5 cut(s)	75, 691, 861, 980, 1015
MaeIII	GTNAC	6 cut(s)	628, 656, 670, 687, 1151, 1205
MalI	GATC	6 cut(s)	721, 972, 1002, 1028, 1259, 1593
MbiI	CCGCTC	2 cut(s)	89, 202
MboI	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
MboII	GAAGA	6 cut(s)	391, 435, 851, 919, 1046, 1193
MhlI	GDGCHC	3 cut(s)	34, 212, 459
MlsI	TGGCCA	1 cut(s)	1550
MluCI	AATT	5 cut(s)	355, 933, 942, 1223, 1534
MluI	ACGCGT	1 cut(s)	219
MluNI	TGGCCA	1 cut(s)	1550
MlyI	GAGTC	3 cut(s)	182, 311, 1657
MmeI	TCCRAC	3 cut(s)	141, 204, 893
Mox20I	TGGCCA	1 cut(s)	1550
MroNI	GCCGGC	3 cut(s)	122, 307, 610
MscI	TGGCCA	1 cut(s)	1550
MseI	TTAA	8 cut(s)	831, 882, 1095, 1470, 1494, 1515, 1538, 1698
MslI	CAYNNNNRTG	5 cut(s)	68, 840, 1272, 1404, 1556
Msp20I	TGGCCA	1 cut(s)	1550
MspA1I	CMGCKG	4 cut(s)	147, 153, 268, 1322
MspCI	CTTAAG	1 cut(s)	1697
MspI	CCGG	6 cut(s)	123, 197, 214, 243, 308, 611
MspR9I	CCNGG	3 cut(s)	163, 214, 244
MvaI	CCWGG	1 cut(s)	163
MvnI	CGCG	5 cut(s)	147, 153, 221, 298, 300
NaeI	GCCGGC	3 cut(s)	124, 309, 612
NciI	CCSGG	2 cut(s)	214, 244
NcoI	CCATGG	1 cut(s)	1554
NdeI	CATATG	1 cut(s)	709
NdeII	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
NgoMIV	GCCGGC	3 cut(s)	122, 307, 610
NlaIII	CATG	7 cut(s)	550, 719, 839, 1133, 1409, 1558, 1687
NlaIV	GGNNCC	9 cut(s)	108, 127, 261, 276, 306, 351, 490, 802, 929
NmeAIII	GCCGAG	2 cut(s)	196, 303
NmuCI	GTSAC	4 cut(s)	628, 656, 670, 687
NotI	GCGGCCGC	2 cut(s)	148, 154
NsbI	TGCGCA	1 cut(s)	444
NspI	RCATGY	1 cut(s)	1409
OliI	CACNNNNGTG	1 cut(s)	1556
PaeR7I	CTCGAG	1 cut(s)	394
PauI	GCGCGC	1 cut(s)	296
PdiI	GCCGGC	3 cut(s)	124, 309, 612
PfeI	GAWTC	1 cut(s)	1418
PflMI	CCANNNNNTGG	1 cut(s)	938
PleI	GAGTC	3 cut(s)	181, 311, 1657
PpsI	GAGTC	3 cut(s)	181, 311, 1657
Ppu21I	YACGTR	2 cut(s)	692, 1016
PpuMI	RGGWCCY	1 cut(s)	801
PshBI	ATTAAT	1 cut(s)	1470
Psp124BI	GAGCTC	2 cut(s)	34, 212
Psp5II	RGGWCCY	1 cut(s)	801
Psp6I	CCWGG	1 cut(s)	161
PspFI	CCCAGC	3 cut(s)	132, 282, 1261
PspGI	CCWGG	1 cut(s)	161
PspN4I	GGNNCC	9 cut(s)	108, 127, 261, 276, 306, 351, 490, 802, 929
PspPI	GGNCC	2 cut(s)	779, 801
PspPPI	RGGWCCY	1 cut(s)	801
PspXI	VCTCGAGB	1 cut(s)	394
PsrI	GAACNNNNNNTAC	2 cut(s)	1182, 1214
PstI	CTGCAG	1 cut(s)	744
PstNI	CAGNNNCTG	1 cut(s)	1421
PteI	GCGCGC	1 cut(s)	296
PvuII	CAGCTG	1 cut(s)	1322
RsaI	GTAC	5 cut(s)	389, 503, 1014, 1455, 1580
RsaNI	GTAC	5 cut(s)	388, 502, 1013, 1454, 1579
RseI	CAYNNNNRTG	5 cut(s)	68, 840, 1272, 1404, 1556
SacI	GAGCTC	2 cut(s)	34, 212
SacII	CCGCGG	2 cut(s)	148, 154
SalI	GTCGAC	1 cut(s)	77
SaqAI	TTAA	8 cut(s)	831, 882, 1095, 1470, 1494, 1515, 1538, 1698
Sau3AI	GATC	6 cut(s)	719, 970, 1000, 1026, 1257, 1591
Sau96I	GGNCC	2 cut(s)	779, 801
ScaI	AGTACT	1 cut(s)	503
SchI	GAGTC	3 cut(s)	182, 311, 1657
ScrFI	CCNGG	3 cut(s)	163, 214, 244
SduI	GDGCHC	3 cut(s)	34, 212, 459
SfaNI	GCATC	4 cut(s)	739, 832, 1036, 1363
SfcI	CTRYAG	4 cut(s)	740, 1035, 1188, 1619
SfiI	GGCCNNNNNGGCC	1 cut(s)	176
Sfr274I	CTCGAG	1 cut(s)	394
Sfr303I	CCGCGG	2 cut(s)	148, 154
SgrBI	CCGCGG	2 cut(s)	148, 154
SgrDI	CGTCGACG	1 cut(s)	77
SinI	GGWCC	1 cut(s)	801
SlaI	CTCGAG	1 cut(s)	394
SmiMI	CAYNNNNRTG	5 cut(s)	68, 840, 1272, 1404, 1556
SmlI	CTYRAG	3 cut(s)	394, 476, 1697
SmoI	CTYRAG	3 cut(s)	394, 476, 1697
SnaBI	TACGTA	1 cut(s)	1016
Sse9I	AATT	5 cut(s)	355, 933, 942, 1223, 1534
SspI	AATATT	1 cut(s)	1099
SspMI	CTAG	1 cut(s)	1595
SstI	GAGCTC	2 cut(s)	34, 212
StyD4I	CCNGG	3 cut(s)	161, 212, 242
StyI	CCWWGG	1 cut(s)	1554
TaaI	ACNGT	5 cut(s)	514, 556, 634, 705, 1117
TaiI	ACGT	5 cut(s)	78, 694, 864, 983, 1018
TaqI	TCGA	3 cut(s)	78, 395, 1029
TasI	AATT	5 cut(s)	355, 933, 942, 1223, 1534
TatI	WGTACW	2 cut(s)	387, 501
TauI	GCSGC	9 cut(s)	89, 147, 150, 153, 156, 159, 205, 265, 268
TfiI	GAWTC	1 cut(s)	1418
Tru1I	TTAA	8 cut(s)	831, 882, 1095, 1470, 1494, 1515, 1538, 1698
Tru9I	TTAA	8 cut(s)	831, 882, 1095, 1470, 1494, 1515, 1538, 1698
TscAI	CASTG	1 cut(s)	409
TseFI	GTSAC	4 cut(s)	628, 656, 670, 687
TseI	GCWGC	2 cut(s)	752, 1640
Tsp45I	GTSAC	4 cut(s)	628, 656, 670, 687
TspDTI	ATGAA	5 cut(s)	704, 852, 880, 1007, 1443
TspGWI	ACGGA	2 cut(s)	119, 362
TspRI	CASTG	1 cut(s)	409
Van91I	CCANNNNNTGG	1 cut(s)	938
Vha464I	CTTAAG	1 cut(s)	1697
VpaK11BI	GGWCC	1 cut(s)	801
VspI	ATTAAT	1 cut(s)	1470
XapI	RAATTY	3 cut(s)	355, 1223, 1534
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	1 cut(s)	1409
XcmI	CCANNNNNNNNNTGG	1 cut(s)	67
XhoI	CTCGAG	1 cut(s)	394
XmiI	GTMKAC	1 cut(s)	78
XspI	CTAG	1 cut(s)	1595
ZraI	GACGTC	1 cut(s)	76
ZrmI	AGTACT	1 cut(s)	503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.