AT5G44495

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000547
Physical Location & Seq
Reverse (-)
17926302 .. 17926710
409 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44495.1

Sequence Viewer

Length: 360 bp
ATGGCTATAAAAAACGTGATCTTATTTCTAGCGGTTTTATGCATTGCATTGTCTGTAAATGCCCAATTACCACAATTTCCGGCACTTTTTCCTTTTCCATTTCCATTTCTACCGAGTCCCGGTGTACCAGGATTACCTGATATAACAAAATGTTTATCATCTGTGATAAATATTCCAGGGTGCATCGTAGAGTTTTCGCAATCCATACTCTTAGAAAAGTTCGGCAAGATAGGTTCAGCTTGTTGCAAAGCAATTTTGGAAGCCGAAACCAATTGTATTCCGCAACTTCCGTTCAATCCGTTTTTTCCTCCTATGCTAAAGGAACAATGTTCAAAAACTGTTGGCGGAGCCTCCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

119

Amino Acids

12.76

Weight (kDa)

7.51

Isoelectric Point (pI)

60.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 50 - 111 3.9e-09 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 32, 281, 345
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 2 cut(s) 295, 333
AjnI CCWGG 2 cut(s) 127, 175
AluBI AGCT 1 cut(s) 239
AluI AGCT 1 cut(s) 239
AsuC2I CCSGG 1 cut(s) 120
BciT130I CCWGG 2 cut(s) 129, 177
BcnI CCSGG 1 cut(s) 120
BfaI CTAG 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 120, 129, 177
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 3 cut(s) 120, 129, 177
BmsI GCATC 1 cut(s) 192
BpuMI CCSGG 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 2 cut(s) 129, 177
BseDI CCNNGG 1 cut(s) 176
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 42
BsiSI CCGG 2 cut(s) 80, 120
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 102
Bsp143I GATC 1 cut(s) 18
BspACI CCGC 3 cut(s) 32, 281, 345
BspLI GGNNCC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 42
BssECI CCNNGG 1 cut(s) 176
BssMI GATC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 129, 177
Bst4CI ACNGT 1 cut(s) 340
BstDEI CTNAG 1 cut(s) 211
BstKTI GATC 1 cut(s) 21
BstMBI GATC 1 cut(s) 18
BstNI CCWGG 2 cut(s) 129, 177
BstSCI CCNGG 3 cut(s) 118, 127, 175
Csp6I GTAC 1 cut(s) 125
CviJI RGCY 4 cut(s) 5, 239, 263, 350
CviKI_1 RGCY 4 cut(s) 5, 239, 263, 350
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 1 cut(s) 211
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EciI GGCGGA 1 cut(s) 360
EcoRII CCWGG 2 cut(s) 127, 175
EcoT22I ATGCAT 1 cut(s) 44
FaiI YATR 5 cut(s) 8, 40, 143, 206, 314
FaqI GGGAC 1 cut(s) 102
FspBI CTAG 1 cut(s) 29
HapII CCGG 2 cut(s) 80, 120
HinfI GANTC 1 cut(s) 115
HpaII CCGG 2 cut(s) 80, 120
Hpy166II GTNNAC 1 cut(s) 125
Hpy8I GTNNAC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 15
HpyCH4V TGCA 4 cut(s) 42, 47, 183, 246
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 1 cut(s) 15
Kzo9I GATC 1 cut(s) 18
LmnI GCTCC 1 cut(s) 347
LpnPI CCDG 7 cut(s) 93, 114, 133, 141, 150, 162, 189
LweI GCATC 1 cut(s) 192
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 15
MalI GATC 1 cut(s) 20
MboI GATC 1 cut(s) 18
MfeI CAATTG 1 cut(s) 271
MluCI AATT 4 cut(s) 65, 74, 252, 271
MlyI GAGTC 1 cut(s) 124
MnlI CCTC 1 cut(s) 318
Mph1103I ATGCAT 1 cut(s) 44
MspI CCGG 2 cut(s) 80, 120
MspR9I CCNGG 3 cut(s) 120, 129, 177
MunI CAATTG 1 cut(s) 271
MvaI CCWGG 2 cut(s) 129, 177
NciI CCSGG 1 cut(s) 120
NdeII GATC 1 cut(s) 18
NlaIV GGNNCC 1 cut(s) 349
NsiI ATGCAT 1 cut(s) 44
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 127, 175
PspGI CCWGG 2 cut(s) 127, 175
PspN4I GGNNCC 1 cut(s) 349
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
Sau3AI GATC 1 cut(s) 18
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 3 cut(s) 120, 129, 177
SetI ASST 4 cut(s) 18, 139, 235, 241
SfaNI GCATC 1 cut(s) 192
Sse9I AATT 4 cut(s) 65, 74, 252, 271
SsiI CCGC 3 cut(s) 32, 281, 345
SspI AATATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 3 cut(s) 118, 127, 175
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 18
TasI AATT 4 cut(s) 65, 74, 252, 271
TspGWI ACGGA 2 cut(s) 279, 288
XspI CTAG 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.