RchiOBHm_Chr2g0087141

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000547
Physical Location & Seq
Forward (+)
2013710 .. 2014090
381 bp
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UTR
Exon/CDS
Intron
PRQ46259

Sequence Viewer

Length: 381 bp
ATGGCAATGAAAATTGTACCATTATCTGCACTGCTACTAGTGGCATTACTCACCACCTTGCTATCGCAACCAGGAATAGCCAATGCGCAGTCGCCACAGAGTCCTCCTTCGGTTCCAGGCCTGCTTCCACCAGGAACTCCGAATGATGTCCTTAAGTGTTGGTCATCTCTTGCCGGCATTAGTGGATGTGTTTTGGAAATCTTTCAATCTGTTTTCAGCTTGCAATTAGGCACCATTGGTGCCGACTGTTGCAAGGCCTTCCTTTCTATCGAGGAAAGCTGTTGGCCCAAAATGTTTCCTCTGACTCCTTTCTTCCCTCCCTTGCTCAAGAACATTTGTGAGCTCCAAGGAAGAGGTGCTGCGCCCCCAGCATTCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

126

Amino Acids

13.24

Weight (kDa)

6.08

Isoelectric Point (pI)

72.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 52 - 113 1.2e-16 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 87
AccB1I GGYRCC 2 cut(s) 230, 239
AfaI GTAC 1 cut(s) 18
AflII CTTAAG 1 cut(s) 152
AgsI TTSAA 1 cut(s) 206
AhlI ACTAGT 1 cut(s) 37
AjnI CCWGG 3 cut(s) 70, 115, 130
AluBI AGCT 3 cut(s) 219, 279, 343
AluI AGCT 3 cut(s) 219, 279, 343
Alw21I GWGCWC 1 cut(s) 345
AoxI GGCC 3 cut(s) 118, 255, 284
ApeKI GCWGC 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 88, 364
AspS9I GGNCC 1 cut(s) 285
AsuHPI GGTGA 1 cut(s) 43
BanI GGYRCC 2 cut(s) 230, 239
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 346
BciT130I CCWGG 3 cut(s) 72, 117, 132
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 1 cut(s) 38
BfrI CTTAAG 1 cut(s) 152
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
Bme1390I CCNGG 3 cut(s) 72, 117, 132
BmgT120I GGNCC 1 cut(s) 285
BmiI GGNNCC 3 cut(s) 114, 232, 241
BmrFI CCNGG 3 cut(s) 72, 117, 132
BpuEI CTTGAG 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 346
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 3 cut(s) 72, 117, 132
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 191
BseMI GCAATG 1 cut(s) 12
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 1 cut(s) 367
BsgI GTGCAG 1 cut(s) 12
BshFI GGCC 3 cut(s) 120, 257, 286
BshNI GGYRCC 2 cut(s) 230, 239
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 174
BsmI GAATGC 1 cut(s) 371
BsnI GGCC 3 cut(s) 120, 257, 286
Bsp1286I GDGCHC 1 cut(s) 345
BspANI GGCC 3 cut(s) 120, 257, 286
BspLI GGNNCC 3 cut(s) 114, 232, 241
BspT107I GGYRCC 2 cut(s) 230, 239
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 346
Bst2UI CCWGG 3 cut(s) 72, 117, 132
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 1 cut(s) 346
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 3 cut(s) 122, 175, 221
BstF5I GGATG 1 cut(s) 191
BstHHI GCGC 2 cut(s) 88, 364
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNI CCWGG 3 cut(s) 72, 117, 132
BstSCI CCNGG 3 cut(s) 70, 115, 130
BstV1I GCAGC 1 cut(s) 346
BsuRI GGCC 3 cut(s) 120, 257, 286
BtsCI GGATG 1 cut(s) 191
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 3 cut(s) 122, 175, 221
CfoI GCGC 2 cut(s) 88, 364
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 17
CviJI RGCY 7 cut(s) 80, 120, 219, 257, 279, 286, 343
CviKI_1 RGCY 7 cut(s) 80, 120, 219, 257, 279, 286, 343
CviQI GTAC 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 346
EarI CTCTTC 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 1 cut(s) 346
Eco147I AGGCCT 2 cut(s) 120, 257
Eco24I GRGCYC 1 cut(s) 345
Eco53kI GAGCTC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 343
EcoRII CCWGG 3 cut(s) 70, 115, 130
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 1 cut(s) 198
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 1 cut(s) 38
FspI TGCGCA 1 cut(s) 87
GlaI GCGC 2 cut(s) 87, 363
GluI GCNGC 1 cut(s) 360
GsaI CCCAGC 1 cut(s) 371
HaeIII GGCC 3 cut(s) 120, 257, 286
HapII CCGG 1 cut(s) 174
HhaI GCGC 2 cut(s) 88, 364
Hin6I GCGC 2 cut(s) 86, 362
HinP1I GCGC 2 cut(s) 86, 362
HinfI GANTC 2 cut(s) 100, 304
HpaII CCGG 1 cut(s) 174
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 2 cut(s) 141, 303
Hpy188III TCNNGA 1 cut(s) 328
HpyAV CCTTC 2 cut(s) 117, 268
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 3 cut(s) 29, 223, 252
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HspAI GCGC 2 cut(s) 86, 362
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 8 cut(s) 57, 84, 102, 117, 129, 134, 144, 187
Lsp1109I GCAGC 1 cut(s) 346
MaeI CTAG 1 cut(s) 38
MboII GAAGA 2 cut(s) 304, 363
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 2 cut(s) 12, 224
MlyI GAGTC 2 cut(s) 109, 298
MnlI CCTC 5 cut(s) 114, 265, 309, 327, 347
MroNI GCCGGC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 201
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 376
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 72, 117, 132
Mva1269I GAATGC 1 cut(s) 371
MvaI CCWGG 3 cut(s) 72, 117, 132
MwoI GCNNNNNNNGC 1 cut(s) 368
NaeI GCCGGC 1 cut(s) 175
NgoMIV GCCGGC 1 cut(s) 173
NlaIV GGNNCC 3 cut(s) 114, 232, 241
NsbI TGCGCA 1 cut(s) 87
PceI AGGCCT 2 cut(s) 120, 257
PctI GAATGC 1 cut(s) 371
PdiI GCCGGC 1 cut(s) 175
PdmI GAANNNNTTC 1 cut(s) 201
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 2 cut(s) 108, 298
PpsI GAGTC 2 cut(s) 108, 298
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 70, 115, 130
PspFI CCCAGC 1 cut(s) 367
PspGI CCWGG 3 cut(s) 70, 115, 130
PspN4I GGNNCC 3 cut(s) 114, 232, 241
PspPI GGNCC 1 cut(s) 285
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 376
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 1 cut(s) 360
Sau96I GGNCC 1 cut(s) 285
SchI GAGTC 2 cut(s) 109, 298
ScrFI CCNGG 3 cut(s) 72, 117, 132
SduI GDGCHC 1 cut(s) 345
SetI ASST 5 cut(s) 59, 221, 281, 345, 358
SmiMI CAYNNNNRTG 1 cut(s) 376
SmlI CTYRAG 2 cut(s) 152, 326
SmoI CTYRAG 2 cut(s) 152, 326
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 2 cut(s) 12, 224
SseBI AGGCCT 2 cut(s) 120, 257
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 345
StuI AGGCCT 2 cut(s) 120, 257
StyD4I CCNGG 3 cut(s) 70, 115, 130
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 270
TasI AATT 2 cut(s) 12, 224
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 36
TseI GCWGC 1 cut(s) 359
TspDTI ATGAA 1 cut(s) 23
TspRI CASTG 1 cut(s) 36
Vha464I CTTAAG 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.