Rorug02G0295500

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000547
Physical Location & Seq
Reverse (-)
32688495 .. 32693301
4807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0295500.1

Sequence Viewer

Length: 1338 bp
ATGGGCGACAGTTATGTCATTCACACCCCAAGTATTTCCAACAAACAGAGCCCCAAGAAAACAAAGAAGAAAAAGCGTGGGGGGAGTAAGAAGAAAATGACCACTGAGCAGACTCTGGCTTTCAAGTCTGTGAGTGAATGGGTTTATTTGGATCAAGCTTCTTCACCGGCTTCCACTGCAGCTTCTTGTGTTGTGGATGATTTTGGGGTTCAGAAGACTCTCGGAAGAGGTGGAGAGAAGGTAGTGTTTGAATTGCATTCACATTCAAAATGCAGTGATGGCTTTCTTTCGCCTTCCAAGTTGGTGGAAAGGGCTCATGGAAATGGGGTGAAAGTTCTTGCTCTGACAGATCATGACACAATGTCTGGCATCCCTGAGGCCCTAGAAGTAGCTCGGAGATTTGGCATGAAGATAATTCCTGGTGTTGAAATCAGTACAATTTTCCAACCAAGAGGAGAGTCTGAACCGGAGGAACCAGTGCACATCCTTGCATATTACAGCAGCTGCGGGCCAACAAGTTTTGAAGAGCTTGAAAAGTTCTTGTCTAATATAAGGGATGGTCGTTATCTTCGTGCAAAGAATATGGTCTCCAAATTGAACAAGCTTAAGTTTCCTCTTACGTGGGAGCATGTTGCAAGGGTTGCAGGCAAGGGAGTTGCTCCTGGGAGATTGCATGTGGCCCGAGCCATGGTTGAAGCCGGTTATGTGGAAAATCTGAAACAAGCTTTTGCCCGCTATCTTTTCGATGGTGGACCTGCATATGCTACGGGAAGTGAACCGCCAACAGAGGATGCAATAAAAATGATATGTGATACGGGAGGTGTGGCTGTGCTAGCTCATCCATGGGCATTGAAAAATACAGTTGCCATCATCAGAAGTTTGAAAGAAGCTGGTCTTCATGGGATGGAAGTTTACAGAAGTGATGGAAAACTGACTGCATACAGTGATCTAGCTGATACTTACGGTCTTCTGAAGCTAGGAGGGTCAGATTACCATGGCAAAGGTGGACATGGTGAGTCTGAGCTGGGTAGTGTGAACCTTCCAGTTTTGGTTTTGCGTGACTTTCTTAAAGTAGCTCGACCAATTTGGTGTGGTGCCATTAGTCACATATTAGAGAACTATGCTGACGAACCTTCAGATTCAAATCTAGCAAGGATTACAAGGTTTGGGAAGGGAGGTTCCCCTTTAAGCTGCGGCAAGGACATGATTGATCGTTGCTTGTTTTTGTGGTTGACAAATGAAGAGAGGCAAAATGCAGTTTTTGAGGCCATTAAACTGAAGCTTTCTCGTATTTCAATTAATCACGGAGGAAACCAGGTTGCTATAGAGAGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

445

Amino Acids

48.52

Weight (kDa)

8.93

Isoelectric Point (pI)

38.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHP PF02811 84 - 161 5.5e-13 PHP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 763
AccB1I GGYRCC 1 cut(s) 1094
AciI CCGC 4 cut(s) 507, 733, 779, 1194
AclWI GGATC 1 cut(s) 159
AcuI CTGAAG 3 cut(s) 992, 1119, 1298
AfaI GTAC 1 cut(s) 436
AfiI CCNNNNNNNGG 1 cut(s) 688
AflII CTTAAG 1 cut(s) 605
AhdI GACNNNNNGTC 1 cut(s) 361
AjnI CCWGG 3 cut(s) 418, 661, 1314
AloI GAACNNNNNNTCC 2 cut(s) 1162, 1194
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 1 cut(s) 592
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 159
AlwNI CAGNNNCTG 2 cut(s) 115, 504
Ama87I CYCGRG 1 cut(s) 681
AoxI GGCC 4 cut(s) 378, 509, 678, 1266
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 4 cut(s) 179, 501, 504, 1191
AseI ATTAAT 1 cut(s) 1299
AspS9I GGNCC 4 cut(s) 379, 509, 679, 752
AsuHPI GGTGA 3 cut(s) 156, 340, 1025
AsuNHI GCTAGC 1 cut(s) 832
AvaI CYCGRG 1 cut(s) 681
AvaII GGWCC 1 cut(s) 752
AxyI CCTNAGG 1 cut(s) 375
BaeGI GKGCMC 1 cut(s) 483
BanI GGYRCC 1 cut(s) 1094
BanII GRGCYC 2 cut(s) 53, 316
BbsI GAAGAC 3 cut(s) 221, 887, 959
Bbv12I GWGCWC 1 cut(s) 483
BbvI GCAGC 4 cut(s) 191, 491, 513, 1178
BccI CCATC 6 cut(s) 272, 551, 740, 875, 898, 917
BciT130I CCWGG 3 cut(s) 420, 663, 1316
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 5 cut(s) 383, 833, 950, 977, 1148
BfmI CTRYAG 2 cut(s) 177, 1323
BfrI CTTAAG 1 cut(s) 605
BfuAI ACCTGC 1 cut(s) 763
BisI GCNGC 5 cut(s) 180, 502, 505, 1192, 1195
BlsI GCNGC 5 cut(s) 181, 503, 506, 1193, 1196
Bme1390I CCNGG 3 cut(s) 420, 663, 1316
Bme18I GGWCC 1 cut(s) 752
BmeRI GACNNNNNGTC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 681
BmgT120I GGNCC 4 cut(s) 379, 509, 679, 752
BmiI GGNNCC 3 cut(s) 474, 1096, 1180
BmrFI CCNGG 3 cut(s) 420, 663, 1316
BmsI GCATC 2 cut(s) 378, 781
BmtI GCTAGC 1 cut(s) 836
BpiI GAAGAC 3 cut(s) 221, 887, 959
BsaAI YACGTR 1 cut(s) 621
BsaBI GATNNNNATC 1 cut(s) 1143
BsaI GGTCTC 1 cut(s) 592
BsaJI CCNNGG 4 cut(s) 662, 687, 842, 994
BsaWI WCCGGW 1 cut(s) 466
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 1 cut(s) 688
Bse118I RCCGGY 2 cut(s) 166, 698
Bse1I ACTGG 2 cut(s) 476, 1043
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 1143
BseBI CCWGG 3 cut(s) 420, 663, 1316
BseDI CCNNGG 4 cut(s) 662, 687, 842, 994
BseGI GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BseJI GATNNNNATC 1 cut(s) 1143
BseLI CCNNNNNNNGG 1 cut(s) 688
BseMII CTCAG 3 cut(s) 96, 366, 1011
BseNI ACTGG 2 cut(s) 476, 1043
BseRI GAGGAG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 4 cut(s) 191, 491, 513, 1178
BseYI CCCAGC 1 cut(s) 1024
BshFI GGCC 4 cut(s) 380, 511, 680, 1268
BshNI GGYRCC 1 cut(s) 1094
BsiHKAI GWGCWC 1 cut(s) 483
BsiHKCI CYCGRG 1 cut(s) 681
BsiSI CCGG 3 cut(s) 167, 467, 699
BslI CCNNNNNNNGG 1 cut(s) 688
BsmAI GTCTC 1 cut(s) 592
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 4 cut(s) 380, 511, 680, 1268
Bso31I GGTCTC 1 cut(s) 592
BsoBI CYCGRG 1 cut(s) 681
Bsp1286I GDGCHC 3 cut(s) 53, 316, 483
Bsp143I GATC 4 cut(s) 151, 349, 946, 1210
Bsp19I CCATGG 3 cut(s) 687, 842, 994
BspACI CCGC 4 cut(s) 507, 733, 779, 1194
BspANI GGCC 4 cut(s) 380, 511, 680, 1268
BspCNI CTCAG 3 cut(s) 97, 367, 1012
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 474, 1096, 1180
BspMAI CTGCAG 1 cut(s) 181
BspMI ACCTGC 1 cut(s) 763
BspOI GCTAGC 1 cut(s) 836
BspPI GGATC 1 cut(s) 159
BspQI GCTCTTC 1 cut(s) 519
BspT107I GGYRCC 1 cut(s) 1094
BspTI CTTAAG 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 592
BsrFI RCCGGY 2 cut(s) 166, 698
BsrI ACTGG 2 cut(s) 476, 1043
BssAI RCCGGY 2 cut(s) 166, 698
BssECI CCNNGG 4 cut(s) 662, 687, 842, 994
BssMI GATC 4 cut(s) 151, 349, 946, 1210
BssT1I CCWWGG 3 cut(s) 687, 842, 994
Bst2UI CCWGG 3 cut(s) 420, 663, 1316
Bst4CI ACNGT 4 cut(s) 11, 862, 944, 965
Bst6I CTCTTC 3 cut(s) 220, 519, 1236
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBAI YACGTR 1 cut(s) 621
BstC8I GCNNGC 4 cut(s) 509, 646, 733, 834
BstDEI CTNAG 3 cut(s) 105, 375, 1020
BstDSI CCRYGG 3 cut(s) 687, 842, 994
BstF5I GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BstKTI GATC 4 cut(s) 154, 352, 949, 1213
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 4 cut(s) 151, 349, 946, 1210
BstMWI GCNNNNNNNGC 4 cut(s) 176, 279, 641, 833
BstNI CCWGG 3 cut(s) 420, 663, 1316
BstNSI RCATGY 2 cut(s) 632, 677
BstSCI CCNGG 3 cut(s) 418, 661, 1314
BstSFI CTRYAG 2 cut(s) 177, 1323
BstSLI GKGCMC 1 cut(s) 483
BstV1I GCAGC 4 cut(s) 191, 491, 513, 1178
BstV2I GAAGAC 3 cut(s) 221, 887, 959
BstXI CCANNNNNNTGG 1 cut(s) 304
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 4 cut(s) 380, 511, 680, 1268
BtgI CCRYGG 3 cut(s) 687, 842, 994
BtsCI GGATG 7 cut(s) 202, 369, 483, 562, 796, 838, 909
BtsI GCAGTG 2 cut(s) 174, 280
BtsIMutI CAGTG 5 cut(s) 102, 174, 280, 483, 949
BveI ACCTGC 1 cut(s) 763
Cac8I GCNNGC 4 cut(s) 509, 646, 733, 834
CaiI CAGNNNCTG 2 cut(s) 115, 504
CciI TCATGA 1 cut(s) 352
Cfr10I RCCGGY 2 cut(s) 166, 698
Cfr13I GGNCC 4 cut(s) 379, 509, 679, 752
CsiI ACCWGGT 1 cut(s) 1314
Csp6I GTAC 1 cut(s) 435
CviQI GTAC 1 cut(s) 435
DdeI CTNAG 3 cut(s) 105, 375, 1020
DpnI GATC 4 cut(s) 153, 351, 948, 1212
DpnII GATC 4 cut(s) 151, 349, 946, 1210
DrdI GACNNNNNNGTC 1 cut(s) 14
DriI GACNNNNNGTC 1 cut(s) 361
DseDI GACNNNNNNGTC 1 cut(s) 14
Eam1104I CTCTTC 3 cut(s) 220, 519, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 361
EarI CTCTTC 3 cut(s) 220, 519, 1236
Eco130I CCWWGG 3 cut(s) 687, 842, 994
Eco24I GRGCYC 2 cut(s) 53, 316
Eco31I GGTCTC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 752
Eco57I CTGAAG 3 cut(s) 992, 1119, 1298
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 681
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 3 cut(s) 418, 661, 1314
EcoT14I CCWWGG 3 cut(s) 687, 842, 994
EcoT38I GRGCYC 2 cut(s) 53, 316
ErhI CCWWGG 3 cut(s) 687, 842, 994
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FauI CCCGC 2 cut(s) 500, 740
FauNDI CATATG 1 cut(s) 760
Fnu4HI GCNGC 5 cut(s) 180, 502, 505, 1192, 1195
FokI GGATG 7 cut(s) 209, 356, 470, 569, 803, 825, 916
FriOI GRGCYC 2 cut(s) 53, 316
Fsp4HI GCNGC 5 cut(s) 180, 502, 505, 1192, 1195
FspBI CTAG 5 cut(s) 383, 833, 950, 977, 1148
GluI GCNGC 5 cut(s) 180, 502, 505, 1192, 1195
GsaI CCCAGC 1 cut(s) 1028
HaeIII GGCC 4 cut(s) 380, 511, 680, 1268
HapII CCGG 3 cut(s) 167, 467, 699
HincII GTYRAC 1 cut(s) 1233
HindII GTYRAC 1 cut(s) 1233
HindIII AAGCTT 4 cut(s) 156, 602, 723, 1280
HinfI GANTC 5 cut(s) 112, 217, 458, 1016, 1139
HpaII CCGG 3 cut(s) 167, 467, 699
HphI GGTGA 3 cut(s) 156, 340, 1025
Hpy166II GTNNAC 7 cut(s) 481, 752, 776, 913, 1007, 1036, 1233
Hpy188III TCNNGA 1 cut(s) 353
Hpy8I GTNNAC 7 cut(s) 481, 752, 776, 913, 1007, 1036, 1233
HpyAV CCTTC 5 cut(s) 232, 303, 1049, 1143, 1165
HpyCH4III ACNGT 4 cut(s) 11, 862, 944, 965
HpyCH4IV ACGT 1 cut(s) 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 279, 641, 833
HpyF3I CTNAG 3 cut(s) 105, 375, 1020
HpySE526I ACGT 1 cut(s) 620
Kzo9I GATC 4 cut(s) 151, 349, 946, 1210
LguI GCTCTTC 1 cut(s) 519
LmnI GCTCC 2 cut(s) 625, 664
Lsp1109I GCAGC 4 cut(s) 191, 491, 513, 1178
LweI GCATC 2 cut(s) 378, 781
MabI ACCWGGT 1 cut(s) 1314
MaeI CTAG 5 cut(s) 383, 833, 950, 977, 1148
MaeII ACGT 1 cut(s) 620
MaeIII GTNAC 2 cut(s) 1058, 1103
MalI GATC 4 cut(s) 153, 351, 948, 1212
MboI GATC 4 cut(s) 151, 349, 946, 1210
MhlI GDGCHC 3 cut(s) 53, 316, 483
MluCI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
MlyI GAGTC 4 cut(s) 106, 211, 467, 1025
MmeI TCCRAC 2 cut(s) 63, 469
MseI TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 504
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 3 cut(s) 167, 467, 699
MspR9I CCNGG 3 cut(s) 420, 663, 1316
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 3 cut(s) 420, 663, 1316
MwoI GCNNNNNNNGC 4 cut(s) 176, 279, 641, 833
NcoI CCATGG 3 cut(s) 687, 842, 994
NdeI CATATG 1 cut(s) 760
NdeII GATC 4 cut(s) 151, 349, 946, 1210
NheI GCTAGC 1 cut(s) 832
NlaIV GGNNCC 3 cut(s) 474, 1096, 1180
NmuCI GTSAC 2 cut(s) 1058, 1103
NspI RCATGY 2 cut(s) 632, 677
PagI TCATGA 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 519
PcsI WCGNNNNNNNCGW 1 cut(s) 568
PctI GAATGC 1 cut(s) 256
PfeI GAWTC 1 cut(s) 1139
PkrI GCNGC 5 cut(s) 181, 503, 506, 1193, 1196
PleI GAGTC 4 cut(s) 106, 211, 466, 1024
PpsI GAGTC 4 cut(s) 106, 211, 466, 1024
Ppu21I YACGTR 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 1299
Psp6I CCWGG 3 cut(s) 418, 661, 1314
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 3 cut(s) 418, 661, 1314
PspN4I GGNNCC 3 cut(s) 474, 1096, 1180
PspPI GGNCC 4 cut(s) 379, 509, 679, 752
PstI CTGCAG 1 cut(s) 181
PstNI CAGNNNCTG 2 cut(s) 115, 504
PvuII CAGCTG 1 cut(s) 504
RsaI GTAC 1 cut(s) 436
RsaNI GTAC 1 cut(s) 435
RseI CAYNNNNRTG 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 519
SaqAI TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
SatI GCNGC 5 cut(s) 180, 502, 505, 1192, 1195
Sau3AI GATC 4 cut(s) 151, 349, 946, 1210
Sau96I GGNCC 4 cut(s) 379, 509, 679, 752
SchI GAGTC 4 cut(s) 106, 211, 467, 1025
ScrFI CCNGG 3 cut(s) 420, 663, 1316
SduI GDGCHC 3 cut(s) 53, 316, 483
SexAI ACCWGGT 1 cut(s) 1314
SfaNI GCATC 2 cut(s) 378, 781
SfcI CTRYAG 2 cut(s) 177, 1323
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
Sse9I AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
SsiI CCGC 4 cut(s) 507, 733, 779, 1194
SspMI CTAG 5 cut(s) 383, 833, 950, 977, 1148
StyD4I CCNGG 3 cut(s) 418, 661, 1314
StyI CCWWGG 3 cut(s) 687, 842, 994
TaaI ACNGT 4 cut(s) 11, 862, 944, 965
TaiI ACGT 1 cut(s) 623
TaqI TCGA 2 cut(s) 744, 1078
TasI AATT 6 cut(s) 251, 414, 438, 593, 1083, 1296
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 1197
TfiI GAWTC 1 cut(s) 1139
Tru1I TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
Tru9I TTAA 5 cut(s) 606, 1068, 1187, 1272, 1299
TscAI CASTG 5 cut(s) 109, 181, 280, 483, 949
TseFI GTSAC 2 cut(s) 1058, 1103
TseI GCWGC 4 cut(s) 179, 501, 504, 1191
Tsp45I GTSAC 2 cut(s) 1058, 1103
TspDTI ATGAA 3 cut(s) 422, 887, 1254
TspGWI ACGGA 1 cut(s) 1320
TspRI CASTG 5 cut(s) 109, 181, 280, 483, 949
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 752
VspI ATTAAT 1 cut(s) 1299
XceI RCATGY 2 cut(s) 632, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 1001
XspI CTAG 5 cut(s) 383, 833, 950, 977, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.