Rorug01G0472800

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000547
Physical Location & Seq
Forward (+)
56387410 .. 56388867
1458 bp
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UTR
Exon/CDS
Intron
Rorug01G0472800.1

Sequence Viewer

Length: 690 bp
ATGGCTTCTTCCAGCTCCCTTGCTGGAAAGGGAAAACGACATGCCGTTCAGGAGAACTCTCCAGCCTTCTGCTTGAGGATTTTCAGTCGTGAAGATCTCAAAGACGGGAAGAAGGAACTTCCGCCGGCAGCTGTGAGGGAGTATGGACATCATTTGGCAGACCATATATACCTTAAGGTTCCAAATTGTGGAAAACATTGGAAAATAGAGTTGAGAACATCACCTCGTCGTGACCGAATGTGGTTAGAGAAGGGATGGGAAGAGTTTGCCAACTTTTACTTACTGGACCAAGGAGACATGGCAACTTTCAGCTCTGAAGGCGAAAATTCTCATTTCCAAGTAAGCATCTATAGCTGGGATGATATGGAAATAGAATACCCTATTTGCAGTGGAGGTGATATCAGCTCACCAGAGAGAAAAGAAATAGGAGCTGAAGCTGTAGCTCTAGCCATTTCGGTTTCCCCCAGATCCTCTGCTGATGAACGTGGAAGGGCTAAGGTCAGAAGACGAAGAAGGAGAGTAACAGGATCTGAAGCTGAAGCTAAAGCCATTTCAATTTCCCCAAGATCCTCTGCTGATGAACATGAATATGATGTGAGCAGTTTCATGTCGGATAAGCCATTTCTTGATATCAGAATTCCAGCCAAAACCTTGGCCTTTCGTGTGGTAAGCTTTTCTGAACAACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

229

Amino Acids

25.88

Weight (kDa)

7.07

Isoelectric Point (pI)

67.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 32 - 117 1.5e-10 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 188
AciI CCGC 1 cut(s) 122
AclWI GGATC 3 cut(s) 462, 535, 561
AcsI RAATTY 2 cut(s) 325, 636
AcuI CTGAAG 4 cut(s) 336, 453, 552, 558
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 173
AgsI TTSAA 1 cut(s) 555
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 3 cut(s) 462, 535, 561
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 325, 636
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 3 cut(s) 213, 399, 407
AvaII GGWCC 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 511
BbvI GCAGC 1 cut(s) 140
BccI CCATC 1 cut(s) 249
BceAI ACGGC 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 2 cut(s) 446, 688
BfmI CTRYAG 2 cut(s) 349, 438
BfrI CTTAAG 1 cut(s) 173
BglII AGATCT 1 cut(s) 94
BisI GCNGC 1 cut(s) 129
BlsI GCNGC 1 cut(s) 130
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 180
BmsI GCATC 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 511
BpmI CTGGAG 1 cut(s) 45
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 289, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse118I RCCGGY 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 288
BseDI CCNNGG 2 cut(s) 289, 651
BseGI GGATG 2 cut(s) 260, 364
BseLI CCNNNNNNNGG 1 cut(s) 188
BseNI ACTGG 1 cut(s) 288
BseXI GCAGC 1 cut(s) 140
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 1 cut(s) 656
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 288
BsnI GGCC 1 cut(s) 656
Bsp143I GATC 4 cut(s) 94, 467, 527, 566
BspACI CCGC 1 cut(s) 122
BspANI GGCC 1 cut(s) 656
BspLI GGNNCC 1 cut(s) 180
BspPI GGATC 3 cut(s) 462, 535, 561
BspTI CTTAAG 1 cut(s) 173
BsrFI RCCGGY 1 cut(s) 124
BsrI ACTGG 1 cut(s) 288
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 2 cut(s) 289, 651
BssMI GATC 4 cut(s) 94, 467, 527, 566
BssT1I CCWWGG 2 cut(s) 289, 651
Bst6I CTCTTC 1 cut(s) 255
BstAFI CTTAAG 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 126
BstDEI CTNAG 1 cut(s) 495
BstF5I GGATG 2 cut(s) 260, 364
BstKTI GATC 4 cut(s) 97, 470, 530, 569
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 4 cut(s) 94, 467, 527, 566
BstMWI GCNNNNNNNGC 2 cut(s) 318, 351
BstNSI RCATGY 1 cut(s) 44
BstSFI CTRYAG 2 cut(s) 349, 438
BstV1I GCAGC 1 cut(s) 140
BstV2I GAAGAC 1 cut(s) 511
BstX2I RGATCY 4 cut(s) 94, 467, 527, 566
BstXI CCANNNNNNTGG 1 cut(s) 652
BstYI RGATCY 4 cut(s) 94, 467, 527, 566
BsuRI GGCC 1 cut(s) 656
BtsCI GGATG 2 cut(s) 260, 364
BtsI GCAGTG 1 cut(s) 394
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 530
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 1 cut(s) 286
CviAII CATG 4 cut(s) 41, 298, 584, 607
DdeI CTNAG 1 cut(s) 495
DpnI GATC 4 cut(s) 96, 469, 529, 568
DpnII GATC 4 cut(s) 94, 467, 527, 566
Eam1104I CTCTTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 255
EciI GGCGGA 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 289, 651
Eco32I GATATC 2 cut(s) 400, 631
Eco47I GGWCC 1 cut(s) 286
Eco57I CTGAAG 4 cut(s) 336, 453, 552, 558
EcoRI GAATTC 1 cut(s) 636
EcoRV GATATC 2 cut(s) 400, 631
EcoT14I CCWWGG 2 cut(s) 289, 651
ErhI CCWWGG 2 cut(s) 289, 651
FaeI CATG 4 cut(s) 44, 301, 587, 610
FatI CATG 4 cut(s) 40, 297, 583, 606
Fnu4HI GCNGC 1 cut(s) 129
FokI GGATG 2 cut(s) 267, 371
Fsp4HI GCNGC 1 cut(s) 129
FspBI CTAG 2 cut(s) 446, 688
GluI GCNGC 1 cut(s) 129
GsaI CCCAGC 1 cut(s) 358
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 1 cut(s) 656
HapII CCGG 1 cut(s) 125
Hin1II CATG 4 cut(s) 44, 301, 587, 610
HindIII AAGCTT 1 cut(s) 670
HpaII CCGG 1 cut(s) 125
HphI GGTGA 3 cut(s) 213, 399, 407
Hpy188I TCNGA 6 cut(s) 316, 503, 532, 613, 635, 679
Hpy188III TCNNGA 4 cut(s) 50, 89, 230, 626
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 6 cut(s) 76, 106, 244, 311, 483, 507
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 1 cut(s) 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 318, 351
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 4 cut(s) 44, 301, 587, 610
KroI GCCGGC 1 cut(s) 124
KroNI GCCGGC 1 cut(s) 126
Kzo9I GATC 4 cut(s) 94, 467, 527, 566
LmnI GCTCC 2 cut(s) 20, 428
Lsp1109I GCAGC 1 cut(s) 140
LweI GCATC 1 cut(s) 354
MaeI CTAG 2 cut(s) 446, 688
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 2 cut(s) 230, 520
MalI GATC 4 cut(s) 96, 469, 529, 568
MboI GATC 4 cut(s) 94, 467, 527, 566
MboII GAAGA 5 cut(s) 104, 121, 272, 516, 522
MflI RGATCY 4 cut(s) 94, 467, 527, 566
MluCI AATT 4 cut(s) 184, 325, 555, 636
MmeI TCCRAC 1 cut(s) 591
MnlI CCTC 6 cut(s) 69, 129, 234, 386, 481, 580
MroNI GCCGGC 1 cut(s) 124
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 588
MspA1I CMGCKG 1 cut(s) 131
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 318, 351
NaeI GCCGGC 1 cut(s) 126
NdeII GATC 4 cut(s) 94, 467, 527, 566
NgoMIV GCCGGC 1 cut(s) 124
NlaIII CATG 4 cut(s) 44, 301, 587, 610
NlaIV GGNNCC 1 cut(s) 180
NmuCI GTSAC 1 cut(s) 230
NspI RCATGY 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PdiI GCCGGC 1 cut(s) 126
PflMI CCANNNNNTGG 1 cut(s) 188
PkrI GCNGC 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 180
PspPI GGNCC 1 cut(s) 286
PstNI CAGNNNCTG 1 cut(s) 530
PsuI RGATCY 4 cut(s) 94, 467, 527, 566
PvuII CAGCTG 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 588
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 1 cut(s) 129
Sau3AI GATC 4 cut(s) 94, 467, 527, 566
Sau96I GGNCC 1 cut(s) 286
SfaNI GCATC 1 cut(s) 354
SfcI CTRYAG 2 cut(s) 349, 438
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 2 cut(s) 73, 173
SmoI CTYRAG 2 cut(s) 73, 173
Sse9I AATT 4 cut(s) 184, 325, 555, 636
SsiI CCGC 1 cut(s) 122
SspMI CTAG 2 cut(s) 446, 688
StyI CCWWGG 2 cut(s) 289, 651
TaiI ACGT 1 cut(s) 487
TaqII GACCGA 1 cut(s) 249
TasI AATT 4 cut(s) 184, 325, 555, 636
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 128
Tsp45I GTSAC 1 cut(s) 230
TspDTI ATGAA 4 cut(s) 495, 594, 595, 600
TspRI CASTG 1 cut(s) 394
Van91I CCANNNNNTGG 1 cut(s) 188
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 286
XapI RAATTY 2 cut(s) 325, 636
XceI RCATGY 1 cut(s) 44
XspI CTAG 2 cut(s) 446, 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.