RchiOBHm_Chr2g0087131

Prolamin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000547
Physical Location & Seq
Forward (+)
2011440 .. 2011823
384 bp
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UTR
Exon/CDS
Intron
PRQ46258

Sequence Viewer

Length: 384 bp
ATGGCAATGAAAATTGTAGCATTGTCTTACCTGCTGCTAGTGGCATTACTCATCACCACCTTGCTAACCCAACCAGGAATAGCCAATGCGCAGTTGCCACAGACTCCTCCTTCGAATATTCCAGGCCTGTTTCCACCAGGAACTCCGCAGGATGCACTTAAATGTTGGTCATCTCTAACCGGCATTAGTGGATGTGTTACGGAAATCTTTCAATCTGTTTTCAGCTTGCAATTAGGCACCATTGGTGCCGACTGTTGCAAGGCCTTCCTTTCTATCGAGGAAAGCTGTTTGCCCAAAATGTTTCCTCTGACTCCTTTCTTCCCTCCTGTGCTCAAGAACTTTTGTGCTCGCCAAGGAGGTCCTGCTCCCCCAGCAAGAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Prolamin_like

Protein Analysis

127

Amino Acids

13.46

Weight (kDa)

8.27

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prolamin_like PF05617 54 - 115 4.8e-16 Prolamin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 90
Acc36I ACCTGC 1 cut(s) 39
AccB1I GGYRCC 2 cut(s) 236, 245
AciI CCGC 1 cut(s) 146
AgsI TTSAA 1 cut(s) 212
AjnI CCWGG 3 cut(s) 73, 121, 136
AluBI AGCT 2 cut(s) 225, 285
AluI AGCT 2 cut(s) 225, 285
Alw21I GWGCWC 2 cut(s) 333, 349
AoxI GGCC 2 cut(s) 124, 261
ApeKI GCWGC 1 cut(s) 34
ArsI GACNNNNNNTTYG 2 cut(s) 94, 126
Asp700I GAANNNNTTC 1 cut(s) 207
AspLEI GCGC 1 cut(s) 91
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 46
AsuII TTCGAA 1 cut(s) 113
AvaII GGWCC 1 cut(s) 359
BanI GGYRCC 2 cut(s) 236, 245
Bbv12I GWGCWC 2 cut(s) 333, 349
BbvI GCAGC 1 cut(s) 21
BciT130I CCWGG 3 cut(s) 75, 123, 138
BfaI CTAG 2 cut(s) 38, 382
BfuAI ACCTGC 1 cut(s) 39
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 75, 123, 138
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 2 cut(s) 238, 247
BmrFI CCNGG 3 cut(s) 75, 123, 138
BmsI GCATC 1 cut(s) 142
Bpu14I TTCGAA 1 cut(s) 113
BpuEI CTTGAG 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 352
Bse118I RCCGGY 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 3 cut(s) 75, 123, 138
BseDI CCNNGG 1 cut(s) 352
BseGI GGATG 2 cut(s) 157, 197
BseMI GCAATG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 21
BseYI CCCAGC 1 cut(s) 370
BshFI GGCC 2 cut(s) 126, 263
BshNI GGYRCC 2 cut(s) 236, 245
BsiHKAI GWGCWC 2 cut(s) 333, 349
BsiSI CCGG 1 cut(s) 180
BsnI GGCC 2 cut(s) 126, 263
Bsp119I TTCGAA 1 cut(s) 113
Bsp1286I GDGCHC 2 cut(s) 333, 349
BspACI CCGC 1 cut(s) 146
BspANI GGCC 2 cut(s) 126, 263
BspLI GGNNCC 2 cut(s) 238, 247
BspMI ACCTGC 1 cut(s) 39
BspT104I TTCGAA 1 cut(s) 113
BspT107I GGYRCC 2 cut(s) 236, 245
BsrDI GCAATG 1 cut(s) 12
BsrFI RCCGGY 1 cut(s) 179
BssAI RCCGGY 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 352
Bst2UI CCWGG 3 cut(s) 75, 123, 138
Bst4CI ACNGT 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 113
BstC8I GCNNGC 2 cut(s) 227, 349
BstF5I GGATG 2 cut(s) 157, 197
BstHHI GCGC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 371
BstNI CCWGG 3 cut(s) 75, 123, 138
BstSCI CCNGG 3 cut(s) 73, 121, 136
BstV1I GCAGC 1 cut(s) 21
BsuRI GGCC 2 cut(s) 126, 263
BtsCI GGATG 2 cut(s) 157, 197
BveI ACCTGC 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 227, 349
CfoI GCGC 1 cut(s) 91
Cfr10I RCCGGY 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 359
CviJI RGCY 5 cut(s) 83, 126, 225, 263, 285
CviKI_1 RGCY 5 cut(s) 83, 126, 225, 263, 285
Eco130I CCWWGG 1 cut(s) 352
Eco147I AGGCCT 2 cut(s) 126, 263
Eco47I GGWCC 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 359
EcoRII CCWGG 3 cut(s) 73, 121, 136
EcoT14I CCWWGG 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 2 cut(s) 164, 204
Fsp4HI GCNGC 1 cut(s) 35
FspBI CTAG 2 cut(s) 38, 382
FspI TGCGCA 1 cut(s) 90
GlaI GCGC 1 cut(s) 90
GluI GCNGC 1 cut(s) 35
GsaI CCCAGC 1 cut(s) 374
HaeIII GGCC 2 cut(s) 126, 263
HapII CCGG 1 cut(s) 180
HhaI GCGC 1 cut(s) 91
Hin6I GCGC 1 cut(s) 89
HinP1I GCGC 1 cut(s) 89
HinfI GANTC 3 cut(s) 103, 310, 378
HpaII CCGG 1 cut(s) 180
HphI GGTGA 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 1 cut(s) 334
HpyAV CCTTC 2 cut(s) 120, 274
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4V TGCA 3 cut(s) 155, 229, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 371
HspAI GCGC 1 cut(s) 89
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 1 cut(s) 21
LweI GCATC 1 cut(s) 142
MaeI CTAG 2 cut(s) 38, 382
MaeIII GTNAC 1 cut(s) 196
MboII GAAGA 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 333, 349
MluCI AATT 2 cut(s) 12, 230
MlyI GAGTC 2 cut(s) 97, 304
MnlI CCTC 5 cut(s) 117, 271, 315, 333, 350
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 160
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 3 cut(s) 75, 123, 138
MvaI CCWGG 3 cut(s) 75, 123, 138
MwoI GCNNNNNNNGC 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 238, 247
NsbI TGCGCA 1 cut(s) 90
NspV TTCGAA 1 cut(s) 113
PceI AGGCCT 2 cut(s) 126, 263
PdmI GAANNNNTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 378
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 2 cut(s) 97, 304
PpsI GAGTC 2 cut(s) 97, 304
PpuMI RGGWCCY 1 cut(s) 359
Psp5II RGGWCCY 1 cut(s) 359
Psp6I CCWGG 3 cut(s) 73, 121, 136
PspFI CCCAGC 1 cut(s) 370
PspGI CCWGG 3 cut(s) 73, 121, 136
PspN4I GGNNCC 2 cut(s) 238, 247
PspPI GGNCC 1 cut(s) 359
PspPPI RGGWCCY 1 cut(s) 359
RseI CAYNNNNRTG 1 cut(s) 160
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 1 cut(s) 35
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 2 cut(s) 97, 304
ScrFI CCNGG 3 cut(s) 75, 123, 138
SduI GDGCHC 2 cut(s) 333, 349
SetI ASST 5 cut(s) 33, 62, 227, 287, 361
SfaNI GCATC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 113
SinI GGWCC 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 160
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 2 cut(s) 12, 230
SseBI AGGCCT 2 cut(s) 126, 263
SsiI CCGC 1 cut(s) 146
SspI AATATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 38, 382
StuI AGGCCT 2 cut(s) 126, 263
StyD4I CCNGG 3 cut(s) 73, 121, 136
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 254
TaqI TCGA 2 cut(s) 113, 276
TasI AATT 2 cut(s) 12, 230
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TseI GCWGC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 359
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 38, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.