AT5G58720

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001145
Physical Location & Seq
Reverse (-)
23719695 .. 23723175
3481 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58720.1

Sequence Viewer

Length: 1560 bp
ATGAAGCAGAAGAACCAGCATAAGAAGAAGAAGAAGCGATCCTGCGCCGCGAAACCTTCCGGTGATGGAACGACGTCGGACGGGAACAAAAAAGATGTGGAGGAAGAGAGGAAGGACGGTGAAGGAAAGAGGGAGATAGAAAACGTTGGAAAAAATTTTATCGAATCGTTAATGGAAGCCTTTTGCTCGGTTTCAATGGAGGAAGCGATGGCTGCTTATAAGGAAGCAGGCGGAGATCTGAACAAAGCCGCTGAGATTTTATCGGATCTGGTGGAAAGCGGTGATGATCCGTCGACGAGTTCGGTGGCGAGTGGTTCTTCGGGTCAGGAAACAGCGTCTACCTCTGAGTATGGGGCAGGGTCAAGTTCTAGCTGTAGCGAAGATTTGACTAGAGATAGATGGTTTAAGGGAAGCAAGCAGAGTAGAGTTATTGCTGCTACAGGGATGGTTTCTTCTGTGATTGCGAAGGATTACTTGAAGCCAAACCCTGTGCGAAAAGAGTTTCCTATGATGGAACGGTCTAAAGAGCTCTGTGGGAACGGGAAAAAAGCTGCGGATAGAGAGAAAGCTGAACAGTTCTTAAGTTCAATGCTTGGAGATGATTGCGAGCTTAGCATGGCTGTTGTTAGAGATGTTTTGTGTCAATGTGGCTATGATGTCGACATGGCTTTGAATGTCTTGCTTGACATGTCTTCTTCATCAACTGATGATTCATTGAGTGGTAAATGTTTCGGCATAGGAGTCAGCGATAGTCTGGCAGAGTCATCTTTTGATACTGATACTTCTGATTGTGAACTTTTCTGGGGTGGAGACTATAGTCAAAGGGACTACGCAAAAGCTCTCATGAGTTCTCAAGATCCTTTTGCAACTACCCAAGGAATTGATGAGCTTGGTCTTCCACAAAAAGTGTTGGAGTCTCTGTTCAACATTCGCCAGAATCCTAAACATGAATCAAAGACAACGAGTTGGAGGAATGTGGCAAAGAAAATGCAGTCACTTGGCATTGATGCTTCTTCATCTAGTGGGGAAGAGCCTCACCCTAATACGTTTGTGAAGGATGACAGTTATCATGAACTTAGAAAAGGTGCAAATGACCAGTGGAATGTTACGAAGTCCTACTATCAAAAAGCTGCCGAAGCATATTCGAAAGGAGGGAGGGCTCATGCGGCTTACCTTTCTGACAAGGGAAGAGTGGCATCTAAACAAGCTCAACGGGCTGATGAGAGGGCAAGCCAAGATATATTCGTTGCAAGAAACAAAGGTATAGAGAATGTGGTAACCATTGATCTGCATGGTCAGCATGTTAAACCAGCAATGAAGCTACTGAAGCTACATCTCTTATTTGGATCATATGTTCCTTCCATTCAGACTCTACGAGTGATCACAGGATGTGGAGCTTCTGGGTTTGGGAAGTCTAAGGTGAAACAATCAGTGGTAAAGCTGCTAGAAAGAGAAGGAGTTAGGTATTGTGAAGAGAACAGAGGGACACTGCTGATCAAGCTTGACGGAGGTAGTAGAGAGTTCAGTTTCTTAGACACAGAGAGTGACTCTGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

519

Amino Acids

56.65

Weight (kDa)

5.64

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 50 - 91 1.2e-22 At5g58720 UBA-like domain
DUF1771 PF08590 356 - 419 7.2e-15 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
AatII GACGTC 1 cut(s) 77
AccI GTMKAC 3 cut(s) 293, 338, 660
AccII CGCG 1 cut(s) 50
AciI CCGC 6 cut(s) 48, 231, 249, 279, 554, 1166
AclI AACGTT 1 cut(s) 144
AclWI GGATC 5 cut(s) 33, 273, 281, 851, 1354
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 1 cut(s) 1346
AcyI GRCGYC 1 cut(s) 74
AflII CTTAAG 1 cut(s) 580
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 5 cut(s) 195, 478, 588, 673, 925
AloI GAACNNNNNNTCC 4 cut(s) 905, 937, 1338, 1370
Alw21I GWGCWC 1 cut(s) 531
Alw26I GTCTC 2 cut(s) 804, 921
AlwI GGATC 5 cut(s) 33, 273, 281, 851, 1354
ApeKI GCWGC 5 cut(s) 212, 434, 551, 1130, 1441
ApoI RAATTY 1 cut(s) 154
AspLEI GCGC 1 cut(s) 47
AsuHPI GGTGA 5 cut(s) 74, 131, 293, 1028, 1432
AsuII TTCGAA 1 cut(s) 1145
BanII GRGCYC 2 cut(s) 531, 1162
BbsI GAAGAC 2 cut(s) 684, 887
Bbv12I GWGCWC 1 cut(s) 531
BbvI GCAGC 5 cut(s) 199, 421, 538, 1117, 1428
BccI CCATC 5 cut(s) 59, 202, 393, 439, 505
BclI TGATCA 2 cut(s) 1380, 1494
BcoDI GTCTC 2 cut(s) 804, 921
BfaI CTAG 4 cut(s) 369, 390, 1020, 1445
BfmI CTRYAG 3 cut(s) 373, 438, 814
BfrI CTTAAG 1 cut(s) 580
BglII AGATCT 1 cut(s) 235
BisI GCNGC 8 cut(s) 48, 213, 249, 435, 552, 1131, 1167, 1442
BlpI GCTNAGC 1 cut(s) 611
BlsI GCNGC 8 cut(s) 49, 214, 250, 436, 553, 1132, 1168, 1443
BmsI GCATC 2 cut(s) 997, 1205
BoxI GACNNNNGTC 1 cut(s) 816
BpiI GAAGAC 2 cut(s) 684, 887
BplI GAGNNNNNCTC 1 cut(s) 1532
Bpu1102I GCTNAGC 1 cut(s) 611
Bpu14I TTCGAA 1 cut(s) 1145
BpuEI CTTGAG 1 cut(s) 837
BsaHI GRCGYC 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 874
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 905, 935
Bse1I ACTGG 1 cut(s) 1096
Bse3DI GCAATG 1 cut(s) 1320
BseDI CCNNGG 1 cut(s) 874
BseGI GGATG 3 cut(s) 450, 1063, 1394
BseMI GCAATG 1 cut(s) 1320
BseMII CTCAG 2 cut(s) 243, 336
BseNI ACTGG 1 cut(s) 1096
BseXI GCAGC 5 cut(s) 199, 421, 538, 1117, 1428
Bsh1236I CGCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 531
BsiSI CCGG 1 cut(s) 60
BslFI GGGAC 2 cut(s) 839, 1498
BsmAI GTCTC 2 cut(s) 804, 921
BsmFI GGGAC 2 cut(s) 839, 1498
Bsp119I TTCGAA 1 cut(s) 1145
Bsp1286I GDGCHC 2 cut(s) 531, 1162
Bsp143I GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
Bsp1720I GCTNAGC 1 cut(s) 611
BspACI CCGC 6 cut(s) 48, 231, 249, 279, 554, 1166
BspCNI CTCAG 2 cut(s) 244, 337
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 2 cut(s) 843, 1069
BspPI GGATC 5 cut(s) 33, 273, 281, 851, 1354
BspQI GCTCTTC 1 cut(s) 1023
BspT104I TTCGAA 1 cut(s) 1145
BspTI CTTAAG 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 1320
BsrI ACTGG 1 cut(s) 1096
BssECI CCNNGG 1 cut(s) 874
BssMI GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
BssNI GRCGYC 1 cut(s) 74
BssT1I CCWWGG 1 cut(s) 874
Bst4CI ACNGT 4 cut(s) 119, 519, 576, 1064
Bst6I CTCTTC 4 cut(s) 99, 1023, 1183, 1467
BstACI GRCGYC 1 cut(s) 74
BstAFI CTTAAG 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 1145
BstC8I GCNNGC 4 cut(s) 229, 416, 608, 1231
BstDEI CTNAG 6 cut(s) 252, 345, 611, 1076, 1416, 1531
BstEII GGTNACC 1 cut(s) 1276
BstF5I GGATG 3 cut(s) 450, 1063, 1394
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 9 cut(s) 41, 238, 268, 289, 859, 1288, 1349, 1383, 1497
BstMAI GTCTC 2 cut(s) 804, 921
BstMBI GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
BstMWI GCNNNNNNNGC 8 cut(s) 212, 612, 1136, 1166, 1214, 1297, 1327, 1498
BstNSI RCATGY 2 cut(s) 691, 1304
BstPAI GACNNNNGTC 1 cut(s) 816
BstPI GGTNACC 1 cut(s) 1276
BstSFI CTRYAG 3 cut(s) 373, 438, 814
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 5 cut(s) 199, 421, 538, 1117, 1428
BstV2I GAAGAC 2 cut(s) 684, 887
BstX2I RGATCY 3 cut(s) 235, 265, 856
BstYI RGATCY 3 cut(s) 235, 265, 856
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 3 cut(s) 450, 1063, 1394
BtsI GCAGTG 1 cut(s) 1487
BtsIMutI CAGTG 3 cut(s) 1103, 1437, 1487
Cac8I GCNNGC 4 cut(s) 229, 416, 608, 1231
CciI TCATGA 2 cut(s) 843, 1069
CfoI GCGC 1 cut(s) 47
CseI GACGC 1 cut(s) 324
CviAII CATG 9 cut(s) 616, 664, 688, 844, 947, 1070, 1163, 1292, 1301
DdeI CTNAG 6 cut(s) 252, 345, 611, 1076, 1416, 1531
DpnI GATC 9 cut(s) 40, 237, 267, 288, 858, 1287, 1348, 1382, 1496
DpnII GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
Eam1104I CTCTTC 4 cut(s) 99, 1023, 1183, 1467
EarI CTCTTC 4 cut(s) 99, 1023, 1183, 1467
EciI GGCGGA 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 874
Eco24I GRGCYC 2 cut(s) 531, 1162
Eco53kI GAGCTC 1 cut(s) 529
Eco57I CTGAAG 1 cut(s) 1346
Eco91I GGTNACC 1 cut(s) 1276
EcoICRI GAGCTC 1 cut(s) 529
EcoO65I GGTNACC 1 cut(s) 1276
EcoT14I CCWWGG 1 cut(s) 874
EcoT38I GRGCYC 2 cut(s) 531, 1162
ErhI CCWWGG 1 cut(s) 874
FaeI CATG 9 cut(s) 619, 667, 691, 847, 950, 1073, 1166, 1295, 1304
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 839, 1498
FatI CATG 9 cut(s) 615, 663, 687, 843, 946, 1069, 1162, 1291, 1300
FauNDI CATATG 1 cut(s) 1351
FbaI TGATCA 2 cut(s) 1380, 1494
FblI GTMKAC 3 cut(s) 293, 338, 660
Fnu4HI GCNGC 8 cut(s) 48, 213, 249, 435, 552, 1131, 1167, 1442
FokI GGATG 3 cut(s) 457, 1070, 1401
FriOI GRGCYC 2 cut(s) 531, 1162
Fsp4HI GCNGC 8 cut(s) 48, 213, 249, 435, 552, 1131, 1167, 1442
FspBI CTAG 4 cut(s) 369, 390, 1020, 1445
GlaI GCGC 1 cut(s) 46
GluI GCNGC 8 cut(s) 48, 213, 249, 435, 552, 1131, 1167, 1442
HapII CCGG 1 cut(s) 60
HgaI GACGC 1 cut(s) 324
HhaI GCGC 1 cut(s) 47
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 9 cut(s) 619, 667, 691, 847, 950, 1073, 1166, 1295, 1304
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 2 cut(s) 294, 661
HindII GTYRAC 2 cut(s) 294, 661
HindIII AAGCTT 1 cut(s) 1499
HinfI GANTC 9 cut(s) 164, 710, 741, 761, 914, 937, 950, 1369, 1547
HpaII CCGG 1 cut(s) 60
HphI GGTGA 5 cut(s) 74, 131, 293, 1028, 1432
Hpy166II GTNNAC 4 cut(s) 294, 339, 661, 794
Hpy188I TCNGA 8 cut(s) 79, 240, 265, 346, 787, 1180, 1368, 1552
Hpy188III TCNNGA 4 cut(s) 326, 844, 854, 1070
Hpy8I GTNNAC 4 cut(s) 294, 339, 661, 794
Hpy99I CGWCG 4 cut(s) 76, 79, 295, 298
HpyAV CCTTC 7 cut(s) 66, 106, 116, 460, 1048, 1368, 1448
HpyCH4III ACNGT 4 cut(s) 119, 519, 576, 1064
HpyCH4IV ACGT 3 cut(s) 74, 144, 1046
HpyCH4V TGCA 5 cut(s) 866, 991, 1088, 1250, 1291
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 612, 1136, 1166, 1214, 1297, 1327, 1498
HpyF3I CTNAG 6 cut(s) 252, 345, 611, 1076, 1416, 1531
HpySE526I ACGT 3 cut(s) 74, 144, 1046
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 9 cut(s) 619, 667, 691, 847, 950, 1073, 1166, 1295, 1304
HspAI GCGC 1 cut(s) 45
Ksp22I TGATCA 2 cut(s) 1380, 1494
Kzo9I GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
LguI GCTCTTC 1 cut(s) 1023
LmnI GCTCC 1 cut(s) 1394
Lsp1109I GCAGC 5 cut(s) 199, 421, 538, 1117, 1428
LweI GCATC 2 cut(s) 997, 1205
MaeI CTAG 4 cut(s) 369, 390, 1020, 1445
MaeII ACGT 3 cut(s) 74, 144, 1046
MaeIII GTNAC 4 cut(s) 993, 1105, 1276, 1544
MalI GATC 9 cut(s) 40, 237, 267, 288, 858, 1287, 1348, 1382, 1496
MboI GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
MflI RGATCY 3 cut(s) 235, 265, 856
MhlI GDGCHC 2 cut(s) 531, 1162
MluCI AATT 2 cut(s) 154, 879
MlyI GAGTC 5 cut(s) 750, 770, 923, 1363, 1541
MmeI TCCRAC 4 cut(s) 57, 127, 891, 947
MseI TTAA 4 cut(s) 170, 405, 581, 1305
MspA1I CMGCKG 1 cut(s) 251
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 8 cut(s) 212, 612, 1136, 1166, 1214, 1297, 1327, 1498
NdeI CATATG 1 cut(s) 1351
NdeII GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
NlaIII CATG 9 cut(s) 619, 667, 691, 847, 950, 1073, 1166, 1295, 1304
NmuCI GTSAC 2 cut(s) 993, 1544
NspI RCATGY 2 cut(s) 691, 1304
NspV TTCGAA 1 cut(s) 1145
PagI TCATGA 2 cut(s) 843, 1069
PciI ACATGT 1 cut(s) 687
PciSI GCTCTTC 1 cut(s) 1023
PfeI GAWTC 4 cut(s) 164, 710, 937, 950
PkrI GCNGC 8 cut(s) 49, 214, 250, 436, 553, 1132, 1168, 1443
PleI GAGTC 5 cut(s) 749, 769, 922, 1363, 1541
PpsI GAGTC 5 cut(s) 749, 769, 922, 1363, 1541
PscI ACATGT 1 cut(s) 687
PshAI GACNNNNGTC 1 cut(s) 816
PsiI TTATAA 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 531
Psp1406I AACGTT 1 cut(s) 144
PspEI GGTNACC 1 cut(s) 1276
PsuI RGATCY 3 cut(s) 235, 265, 856
SacI GAGCTC 1 cut(s) 531
SalI GTCGAC 2 cut(s) 292, 659
SapI GCTCTTC 1 cut(s) 1023
SaqAI TTAA 4 cut(s) 170, 405, 581, 1305
SatI GCNGC 8 cut(s) 48, 213, 249, 435, 552, 1131, 1167, 1442
Sau3AI GATC 9 cut(s) 38, 235, 265, 286, 856, 1285, 1346, 1380, 1494
SchI GAGTC 5 cut(s) 750, 770, 923, 1363, 1541
SduI GDGCHC 2 cut(s) 531, 1162
SfaNI GCATC 2 cut(s) 997, 1205
SfcI CTRYAG 3 cut(s) 373, 438, 814
SfuI TTCGAA 1 cut(s) 1145
SgrDI CGTCGACG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 580, 852
SmoI CTYRAG 2 cut(s) 580, 852
Sse9I AATT 2 cut(s) 154, 879
SsiI CCGC 6 cut(s) 48, 231, 249, 279, 554, 1166
SspMI CTAG 4 cut(s) 369, 390, 1020, 1445
SstI GAGCTC 1 cut(s) 531
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 4 cut(s) 119, 519, 576, 1064
TaiI ACGT 3 cut(s) 77, 147, 1049
TaqI TCGA 4 cut(s) 162, 293, 660, 1145
TasI AATT 2 cut(s) 154, 879
TauI GCSGC 3 cut(s) 50, 251, 1169
TfiI GAWTC 4 cut(s) 164, 710, 937, 950
Tru1I TTAA 4 cut(s) 170, 405, 581, 1305
Tru9I TTAA 4 cut(s) 170, 405, 581, 1305
TscAI CASTG 3 cut(s) 1103, 1437, 1494
TseFI GTSAC 2 cut(s) 993, 1544
TseI GCWGC 5 cut(s) 212, 434, 551, 1130, 1441
Tsp45I GTSAC 2 cut(s) 993, 1544
TspDTI ATGAA 7 cut(s) 17, 687, 702, 963, 1005, 1086, 1331
TspGWI ACGGA 2 cut(s) 279, 1521
TspRI CASTG 3 cut(s) 1103, 1437, 1494
Vha464I CTTAAG 1 cut(s) 580
XapI RAATTY 1 cut(s) 154
XceI RCATGY 2 cut(s) 691, 1304
XmiI GTMKAC 3 cut(s) 293, 338, 660
XspI CTAG 4 cut(s) 369, 390, 1020, 1445
ZraI GACGTC 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.