FvH4_5g18730

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001145
Physical Location & Seq
Forward (+)
10770225 .. 10770836
612 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18730.t1

Sequence Viewer

Length: 612 bp
ATGCTCGGAGACGAATCGGAGTTAAACTTGGGTGTTGTTAGGGATGTCCTCTCTCTATGTGGCTATGAAGTTGAAAGGGCCTTGGATGCCTTGCTTGAGTTGTCTTCTTCTTCCTCGAGTGAGACGTGGTATTGCGTGGGACTGCTGGGAGACACTGATCAGAGTTATGCAGAAGTTCTTGCCGGTAGCACTTCCGGAGCTCACCGGAGTTCGAGCTCAGATGACCTCTCTAAGATAGTGTTGGAATCTTTGTTCAACAAGTCTAATAAGAGGAGTCGACCTGATAAAGAAGAACCGAGAACCATGAACTCTTGGAAGAAAAGTGCCAAGAAAGTGCAAGACTCTTCGGAATCCAAGCTCGTTGTTGCCAGAGGAGGTGAATATGATGCATATAGAGGGGCTGCAAAGCAGCACTGGGATTCAGTAACGTCCGACTACCAGAAAGCAGCAAATGCATATTCAAACGGATCTCGGGCGTATGCAGGTTATCTCTCTGAGCAGGCGAAAGTGAAGGCGAAACTGGCAAGAGAGGCGGATGAGAAGGCAAGCCAGGAGATCTTCAAAGCAAGAAACAAGGACAAGGGGATAGAGAATGATTGTGATGACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

204

Amino Acids

22.29

Weight (kDa)

5.37

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 128 - 191 1.4e-14 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 277
AccIII TCCGGA 1 cut(s) 194
AciI CCGC 1 cut(s) 533
AclWI GGATC 1 cut(s) 475
AgsI TTSAA 4 cut(s) 74, 256, 462, 562
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 1 cut(s) 549
AloI GAACNNNNNNTCC 2 cut(s) 236, 268
AluBI AGCT 3 cut(s) 200, 216, 358
AluI AGCT 3 cut(s) 200, 216, 358
Alw21I GWGCWC 2 cut(s) 202, 218
Alw26I GTCTC 3 cut(s) 3, 116, 144
AlwI GGATC 1 cut(s) 475
Ama87I CYCGRG 2 cut(s) 115, 471
Aor13HI TCCGGA 1 cut(s) 194
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 401, 409, 446
AspS9I GGNCC 1 cut(s) 78
AsuHPI GGTGA 2 cut(s) 194, 389
AvaI CYCGRG 2 cut(s) 115, 471
BanII GRGCYC 2 cut(s) 202, 218
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 202, 218
BbvI GCAGC 3 cut(s) 388, 421, 458
BciT130I CCWGG 1 cut(s) 551
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 3 cut(s) 3, 116, 144
BfuAI ACCTGC 1 cut(s) 473
BglII AGATCT 1 cut(s) 555
BisI GCNGC 3 cut(s) 402, 410, 447
BlsI GCNGC 3 cut(s) 403, 411, 448
Bme1390I CCNGG 1 cut(s) 551
BmeT110I CYCGRG 2 cut(s) 115, 471
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 551
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 76, 376
BmuI ACTGGG 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 2 cut(s) 194, 204
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 419, 525
BseAI TCCGGA 1 cut(s) 194
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 3 cut(s) 49, 91, 541
BseMII CTCAG 2 cut(s) 231, 486
BseNI ACTGG 2 cut(s) 419, 525
BseRI GAGGAG 2 cut(s) 286, 387
BseXI GCAGC 3 cut(s) 388, 421, 458
BseYI CCCAGC 1 cut(s) 145
BshFI GGCC 1 cut(s) 80
BsiHKAI GWGCWC 2 cut(s) 202, 218
BsiHKCI CYCGRG 2 cut(s) 115, 471
BsiSI CCGG 3 cut(s) 183, 195, 205
BslFI GGGAC 1 cut(s) 153
BsmAI GTCTC 3 cut(s) 3, 116, 144
BsmBI CGTCTC 2 cut(s) 3, 116
BsmFI GGGAC 1 cut(s) 153
BsnI GGCC 1 cut(s) 80
BsoBI CYCGRG 2 cut(s) 115, 471
Bsp1286I GDGCHC 2 cut(s) 202, 218
Bsp13I TCCGGA 1 cut(s) 194
Bsp143I GATC 3 cut(s) 157, 467, 555
BspACI CCGC 1 cut(s) 533
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 230, 487
BspEI TCCGGA 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 2 cut(s) 419, 525
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 3 cut(s) 157, 467, 555
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 349
BstAPI GCANNNNNTGC 1 cut(s) 452
BstC8I GCNNGC 2 cut(s) 501, 547
BstDEI CTNAG 3 cut(s) 217, 231, 495
BstF5I GGATG 3 cut(s) 49, 91, 541
BstKTI GATC 3 cut(s) 160, 470, 558
BstMAI GTCTC 3 cut(s) 3, 116, 144
BstMBI GATC 3 cut(s) 157, 467, 555
BstMWI GCNNNNNNNGC 4 cut(s) 86, 452, 521, 530
BstNI CCWGG 1 cut(s) 551
BstSCI CCNGG 1 cut(s) 549
BstV1I GCAGC 3 cut(s) 388, 421, 458
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 2 cut(s) 467, 555
BstYI RGATCY 2 cut(s) 467, 555
BsuRI GGCC 1 cut(s) 80
BtrI CACGTC 1 cut(s) 126
BtsCI GGATG 3 cut(s) 49, 91, 541
BtsIMutI CAGTG 2 cut(s) 153, 412
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 2 cut(s) 501, 547
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 78
CviAII CATG 1 cut(s) 304
CviJI RGCY 7 cut(s) 63, 80, 200, 216, 358, 401, 549
CviKI_1 RGCY 7 cut(s) 63, 80, 200, 216, 358, 401, 549
DdeI CTNAG 3 cut(s) 217, 231, 495
DpnI GATC 3 cut(s) 159, 469, 557
DpnII GATC 3 cut(s) 157, 467, 555
Eam1104I CTCTTC 1 cut(s) 349
EarI CTCTTC 1 cut(s) 349
EciI GGCGGA 1 cut(s) 548
Ecl136II GAGCTC 2 cut(s) 200, 216
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 2 cut(s) 202, 218
Eco53kI GAGCTC 2 cut(s) 200, 216
Eco88I CYCGRG 2 cut(s) 115, 471
EcoICRI GAGCTC 2 cut(s) 200, 216
EcoO109I RGGNCCY 1 cut(s) 78
EcoRII CCWGG 1 cut(s) 549
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 2 cut(s) 391, 457
EcoT38I GRGCYC 2 cut(s) 202, 218
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 3, 116
FaeI CATG 1 cut(s) 307
FaiI YATR 9 cut(s) 58, 66, 168, 305, 384, 391, 393, 457, 480
FaqI GGGAC 1 cut(s) 153
FatI CATG 1 cut(s) 303
FbaI TGATCA 1 cut(s) 157
FblI GTMKAC 1 cut(s) 277
Fnu4HI GCNGC 3 cut(s) 402, 410, 447
FokI GGATG 3 cut(s) 56, 98, 548
FriOI GRGCYC 2 cut(s) 202, 218
Fsp4HI GCNGC 3 cut(s) 402, 410, 447
GluI GCNGC 3 cut(s) 402, 410, 447
GsaI CCCAGC 1 cut(s) 149
HaeIII GGCC 1 cut(s) 80
HapII CCGG 3 cut(s) 183, 195, 205
Hin1II CATG 1 cut(s) 307
HincII GTYRAC 1 cut(s) 278
HindII GTYRAC 1 cut(s) 278
HinfI GANTC 6 cut(s) 14, 245, 274, 341, 350, 419
HpaII CCGG 3 cut(s) 183, 195, 205
HphI GGTGA 2 cut(s) 194, 389
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 7 cut(s) 8, 19, 162, 220, 349, 433, 496
Hpy188III TCNNGA 1 cut(s) 195
Hpy8I GTNNAC 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 505, 535
HpyCH4IV ACGT 2 cut(s) 125, 428
HpyCH4V TGCA 6 cut(s) 170, 337, 389, 404, 455, 482
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 452, 521, 530
HpyF3I CTNAG 3 cut(s) 217, 231, 495
HpySE526I ACGT 2 cut(s) 125, 428
Hsp92II CATG 1 cut(s) 307
Kpn2I TCCGGA 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 3 cut(s) 157, 467, 555
LmnI GCTCC 1 cut(s) 197
Lsp1109I GCAGC 3 cut(s) 388, 421, 458
LweI GCATC 2 cut(s) 76, 376
MaeII ACGT 2 cut(s) 125, 428
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 159, 469, 557
MboI GATC 3 cut(s) 157, 467, 555
MboII GAAGA 7 cut(s) 96, 99, 102, 302, 328, 336, 550
MflI RGATCY 2 cut(s) 467, 555
MhlI GDGCHC 2 cut(s) 202, 218
MlyI GAGTC 2 cut(s) 283, 335
MmeI TCCRAC 2 cut(s) 222, 456
MnlI CCTC 8 cut(s) 59, 124, 236, 264, 365, 368, 389, 523
Mph1103I ATGCAT 2 cut(s) 391, 457
MroI TCCGGA 1 cut(s) 194
MseI TTAA 1 cut(s) 23
MspI CCGG 3 cut(s) 183, 195, 205
MspR9I CCNGG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 551
MwoI GCNNNNNNNGC 4 cut(s) 86, 452, 521, 530
NdeII GATC 3 cut(s) 157, 467, 555
NlaIII CATG 1 cut(s) 307
NsiI ATGCAT 2 cut(s) 391, 457
PaeR7I CTCGAG 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 4 cut(s) 14, 245, 350, 419
PkrI GCNGC 3 cut(s) 403, 411, 448
PleI GAGTC 2 cut(s) 282, 335
PpsI GAGTC 2 cut(s) 282, 335
Psp124BI GAGCTC 2 cut(s) 202, 218
Psp6I CCWGG 1 cut(s) 549
PspFI CCCAGC 1 cut(s) 145
PspGI CCWGG 1 cut(s) 549
PspPI GGNCC 1 cut(s) 78
PspXI VCTCGAGB 1 cut(s) 115
PsuI RGATCY 2 cut(s) 467, 555
SacI GAGCTC 2 cut(s) 202, 218
SalI GTCGAC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 3 cut(s) 402, 410, 447
Sau3AI GATC 3 cut(s) 157, 467, 555
Sau96I GGNCC 1 cut(s) 78
SchI GAGTC 2 cut(s) 283, 335
ScrFI CCNGG 1 cut(s) 551
SduI GDGCHC 2 cut(s) 202, 218
SetI ASST 9 cut(s) 128, 202, 218, 228, 283, 360, 379, 431, 487
SfaNI GCATC 2 cut(s) 76, 376
Sfr274I CTCGAG 1 cut(s) 115
SlaI CTCGAG 1 cut(s) 115
SmlI CTYRAG 2 cut(s) 95, 115
SmoI CTYRAG 2 cut(s) 95, 115
SsiI CCGC 1 cut(s) 533
SstI GAGCTC 2 cut(s) 202, 218
StyD4I CCNGG 1 cut(s) 549
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 2 cut(s) 128, 431
TaqI TCGA 3 cut(s) 116, 212, 277
TfiI GAWTC 4 cut(s) 14, 245, 350, 419
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 2 cut(s) 160, 419
TseI GCWGC 3 cut(s) 401, 409, 446
TspDTI ATGAA 2 cut(s) 81, 320
TspGWI ACGGA 1 cut(s) 480
TspRI CASTG 2 cut(s) 160, 419
XhoI CTCGAG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 277
Zsp2I ATGCAT 2 cut(s) 391, 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.