Rorug07G0074500

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001145
Physical Location & Seq
Forward (+)
5745000 .. 5745863
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0074500.1

Sequence Viewer

Length: 864 bp
ATGGCTTCTCTTCCCATCCTTACCATCCCTTTCTCTGCTTTGTCTTGCAGAAGACCTCCAAACCCTAAGCCCTCTTCCCCATATCTCTCCCGCCTGGTAAAGGCTCCCAACTACTCCGTTTCTTCCCTCCACCAAAAACAGCACTTCCGATTCTCAGCCAAAAGAACACCAGAGGATGATGACCACTCCAAGTCGGAGCCGCCGAGCAAGGGAAAGGCTGACTCGGAGGCGAGCAAGGAGGTCAACCAGGCCGAGAAGAAACCGGTCGACTCAAAGGCTCCAGGCGAGGCAGAAGTTGAGTCGGAGCCGCCGAGCAAGGGAAAGGCTGACTCGGAGGTCAACAAGGCCGAGAAGAAACCGGTCGACTCAAAGGCTCCAGGCGAGGCAGAAATTGGGTCGGAGGAGATTTCTGACAAAGAGTTGGGTGAGAAGCTGTTGCAGAGGCAACCGATTCAATTCTTGGTGGATGCTTTTGCCAAACGTCCTTGGCCAGGTCAGGGAGAAGTCGAAAGGGAAAAACTAGTGATCGACTGGGCCCGCAGACTAGCCCCGGTCCAACACCCTGAAGAGTTCCCTGAAATGTTCACCTATAAGGATCAGCATTGGATCAAGGCCTATTACGGGAGAGGGTCGGCGAAAATTTTCTTTGAGATGGATTCTACTAATCAGTTTATGAGAAGAAGAATGGCGATCATGTCATTTGCTCTGAACGGGCTAAAGCTACGGGAGTTGAACTGTTTCCCACTGGGAAAATTCCTCGAGCTGCTGTCCATTGCTGATGAAGAGCACGTCAGTGATTTCCAGAGTCTGTTTTCTGAAATGCGGCTTCGGGCAAACAGGATTAATTGGGGTCCTACTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

287

Amino Acids

32.58

Weight (kDa)

8.36

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AccI GTMKAC 2 cut(s) 267, 363
AciI CCGC 5 cut(s) 91, 200, 308, 538, 823
AclWI GGATC 2 cut(s) 603, 614
AcoI YGGCCR 1 cut(s) 488
AcsI RAATTY 2 cut(s) 639, 752
AcuI CTGAAG 1 cut(s) 585
AfiI CCNNNNNNNGG 6 cut(s) 100, 209, 317, 491, 497, 621
AgeI ACCGGT 2 cut(s) 262, 358
AgsI TTSAA 2 cut(s) 455, 733
AhlI ACTAGT 1 cut(s) 520
AjiI CACGTC 1 cut(s) 790
AjnI CCWGG 5 cut(s) 93, 246, 280, 376, 490
AleI CACNNNNGTG 1 cut(s) 792
AluBI AGCT 3 cut(s) 433, 721, 763
AluI AGCT 3 cut(s) 433, 721, 763
Alw21I GWGCWC 1 cut(s) 789
AlwI GGATC 2 cut(s) 603, 614
AlwNI CAGNNNCTG 1 cut(s) 808
Ama87I CYCGRG 1 cut(s) 758
AoxI GGCC 5 cut(s) 249, 345, 488, 534, 612
ApaI GGGCCC 1 cut(s) 538
ApeKI GCWGC 1 cut(s) 763
ApoI RAATTY 2 cut(s) 639, 752
AseI ATTAAT 1 cut(s) 843
AsiGI ACCGGT 2 cut(s) 262, 358
Asp700I GAANNNNTTC 2 cut(s) 641, 737
AspS9I GGNCC 4 cut(s) 534, 535, 553, 851
AsuC2I CCSGG 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 437, 577
AvaI CYCGRG 1 cut(s) 758
AvaII GGWCC 2 cut(s) 553, 851
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 1 cut(s) 490
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 1 cut(s) 750
BccI CCATC 3 cut(s) 23, 32, 646
BciT130I CCWGG 5 cut(s) 95, 248, 282, 378, 492
BcnI CCSGG 1 cut(s) 551
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 3 cut(s) 521, 545, 858
BisI GCNGC 4 cut(s) 200, 308, 764, 824
BlsI GCNGC 4 cut(s) 201, 309, 765, 825
Bme1390I CCNGG 6 cut(s) 95, 248, 282, 378, 492, 551
Bme18I GGWCC 2 cut(s) 553, 851
BmeT110I CYCGRG 1 cut(s) 758
BmgBI CACGTC 1 cut(s) 790
BmgT120I GGNCC 4 cut(s) 534, 535, 553, 851
BmiI GGNNCC 7 cut(s) 105, 198, 279, 306, 375, 536, 852
BmrFI CCNGG 6 cut(s) 95, 248, 282, 378, 492, 551
BmrI ACTGGG 2 cut(s) 541, 755
BmsI GCATC 1 cut(s) 457
BmuI ACTGGG 2 cut(s) 541, 755
BpiI GAAGAC 1 cut(s) 58
BpmI CTGGAG 2 cut(s) 264, 360
Bpu10I CCTNAGC 1 cut(s) 66
BpuMI CCSGG 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 485, 549
BsaWI WCCGGW 2 cut(s) 262, 358
Bsc4I CCNNNNNNNGG 6 cut(s) 100, 209, 317, 491, 497, 621
Bse118I RCCGGY 2 cut(s) 262, 358
Bse1I ACTGG 2 cut(s) 536, 750
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 5 cut(s) 95, 248, 282, 378, 492
BseDI CCNNGG 2 cut(s) 485, 549
BseGI GGATG 4 cut(s) 15, 24, 181, 472
BseLI CCNNNNNNNGG 6 cut(s) 100, 209, 317, 491, 497, 621
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 536, 750
BseRI GAGGAG 1 cut(s) 416
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 1 cut(s) 750
Bsh1285I CGRYCG 2 cut(s) 267, 363
BshFI GGCC 5 cut(s) 251, 347, 490, 536, 614
BshTI ACCGGT 2 cut(s) 262, 358
BsiEI CGRYCG 2 cut(s) 267, 363
BsiHKAI GWGCWC 1 cut(s) 789
BsiHKCI CYCGRG 1 cut(s) 758
BsiSI CCGG 3 cut(s) 263, 359, 551
BslI CCNNNNNNNGG 6 cut(s) 100, 209, 317, 491, 497, 621
BsnI GGCC 5 cut(s) 251, 347, 490, 536, 614
BsoBI CYCGRG 1 cut(s) 758
Bsp120I GGGCCC 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 538, 789
Bsp143I GATC 4 cut(s) 525, 595, 606, 690
BspACI CCGC 5 cut(s) 91, 200, 308, 538, 823
BspANI GGCC 5 cut(s) 251, 347, 490, 536, 614
BspCNI CTCAG 1 cut(s) 167
BspLI GGNNCC 7 cut(s) 105, 198, 279, 306, 375, 536, 852
BspPI GGATC 2 cut(s) 603, 614
BspQI GCTCTTC 1 cut(s) 777
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 2 cut(s) 262, 358
BsrI ACTGG 2 cut(s) 536, 750
BssAI RCCGGY 2 cut(s) 262, 358
BssECI CCNNGG 2 cut(s) 485, 549
BssMI GATC 4 cut(s) 525, 595, 606, 690
BssT1I CCWWGG 1 cut(s) 485
Bst2UI CCWGG 5 cut(s) 95, 248, 282, 378, 492
Bst4CI ACNGT 1 cut(s) 737
Bst6I CTCTTC 4 cut(s) 15, 79, 561, 777
BstC8I GCNNGC 2 cut(s) 232, 538
BstDEI CTNAG 2 cut(s) 66, 154
BstENI CCTNNNNNAGG 2 cut(s) 98, 489
BstF5I GGATG 4 cut(s) 15, 24, 181, 472
BstKTI GATC 4 cut(s) 528, 598, 609, 693
BstMBI GATC 4 cut(s) 525, 595, 606, 690
BstMCI CGRYCG 2 cut(s) 267, 363
BstNI CCWGG 5 cut(s) 95, 248, 282, 378, 492
BstSCI CCNGG 6 cut(s) 93, 246, 280, 376, 490, 549
BstSLI GKGCMC 1 cut(s) 538
BstV1I GCAGC 1 cut(s) 750
BstV2I GAAGAC 1 cut(s) 58
BsuRI GGCC 5 cut(s) 251, 347, 490, 536, 614
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 4 cut(s) 15, 24, 181, 472
BtsIMutI CAGTG 2 cut(s) 743, 799
Cac8I GCNNGC 2 cut(s) 232, 538
CaiI CAGNNNCTG 1 cut(s) 808
Cfr10I RCCGGY 2 cut(s) 262, 358
Cfr13I GGNCC 4 cut(s) 534, 535, 553, 851
CspAI ACCGGT 2 cut(s) 262, 358
CviAII CATG 1 cut(s) 694
DdeI CTNAG 2 cut(s) 66, 154
DpnI GATC 4 cut(s) 527, 597, 608, 692
DpnII GATC 4 cut(s) 525, 595, 606, 690
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 1 cut(s) 488
Eam1104I CTCTTC 4 cut(s) 15, 79, 561, 777
EarI CTCTTC 4 cut(s) 15, 79, 561, 777
Eco130I CCWWGG 1 cut(s) 485
Eco147I AGGCCT 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 553, 851
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 758
EcoNI CCTNNNNNAGG 2 cut(s) 98, 489
EcoO109I RGGNCCY 1 cut(s) 851
EcoRII CCWGG 5 cut(s) 93, 246, 280, 376, 490
EcoT14I CCWWGG 1 cut(s) 485
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 1 cut(s) 697
FaiI YATR 4 cut(s) 82, 591, 674, 695
FatI CATG 1 cut(s) 693
FauI CCCGC 2 cut(s) 98, 545
FblI GTMKAC 2 cut(s) 267, 363
Fnu4HI GCNGC 4 cut(s) 200, 308, 764, 824
FokI GGATG 4 cut(s) 2, 11, 188, 479
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 4 cut(s) 200, 308, 764, 824
FspBI CTAG 3 cut(s) 521, 545, 858
GluI GCNGC 4 cut(s) 200, 308, 764, 824
GsuI CTGGAG 2 cut(s) 264, 360
HaeIII GGCC 5 cut(s) 251, 347, 490, 536, 614
HapII CCGG 3 cut(s) 263, 359, 551
Hin1II CATG 1 cut(s) 697
HincII GTYRAC 4 cut(s) 244, 268, 340, 364
HindII GTYRAC 4 cut(s) 244, 268, 340, 364
HinfI GANTC 9 cut(s) 150, 221, 269, 299, 329, 365, 451, 656, 805
HpaII CCGG 3 cut(s) 263, 359, 551
HphI GGTGA 2 cut(s) 437, 577
Hpy166II GTNNAC 5 cut(s) 244, 268, 340, 364, 585
Hpy188I TCNGA 9 cut(s) 149, 196, 226, 304, 334, 400, 412, 708, 817
Hpy188III TCNNGA 1 cut(s) 802
Hpy8I GTNNAC 5 cut(s) 244, 268, 340, 364, 585
HpyCH4III ACNGT 1 cut(s) 737
HpyCH4IV ACGT 2 cut(s) 481, 789
HpyCH4V TGCA 2 cut(s) 48, 439
HpyF3I CTNAG 2 cut(s) 66, 154
HpySE526I ACGT 2 cut(s) 481, 789
Hsp92II CATG 1 cut(s) 697
Kzo9I GATC 4 cut(s) 525, 595, 606, 690
LguI GCTCTTC 1 cut(s) 777
LmnI GCTCC 5 cut(s) 109, 196, 283, 304, 379
Lsp1109I GCAGC 1 cut(s) 750
LweI GCATC 1 cut(s) 457
MaeI CTAG 3 cut(s) 521, 545, 858
MaeII ACGT 2 cut(s) 481, 789
MalI GATC 4 cut(s) 527, 597, 608, 692
MboI GATC 4 cut(s) 525, 595, 606, 690
MboII GAAGA 9 cut(s) 63, 66, 114, 268, 364, 578, 690, 693, 794
MhlI GDGCHC 2 cut(s) 538, 789
MlsI TGGCCA 1 cut(s) 490
MluCI AATT 5 cut(s) 390, 455, 639, 752, 844
MluNI TGGCCA 1 cut(s) 490
MlyI GAGTC 6 cut(s) 215, 263, 308, 323, 359, 814
MmeI TCCRAC 4 cut(s) 174, 282, 378, 580
Mox20I TGGCCA 1 cut(s) 490
MroXI GAANNNNTTC 2 cut(s) 641, 737
MscI TGGCCA 1 cut(s) 490
MseI TTAA 1 cut(s) 843
MslI CAYNNNNRTG 1 cut(s) 792
Msp20I TGGCCA 1 cut(s) 490
MspI CCGG 3 cut(s) 263, 359, 551
MspR9I CCNGG 6 cut(s) 95, 248, 282, 378, 492, 551
MvaI CCWGG 5 cut(s) 95, 248, 282, 378, 492
NciI CCSGG 1 cut(s) 551
NdeII GATC 4 cut(s) 525, 595, 606, 690
NlaIII CATG 1 cut(s) 697
NlaIV GGNNCC 7 cut(s) 105, 198, 279, 306, 375, 536, 852
NmeAIII GCCGAG 4 cut(s) 228, 277, 336, 373
OliI CACNNNNGTG 1 cut(s) 792
PaeR7I CTCGAG 1 cut(s) 758
PceI AGGCCT 1 cut(s) 614
PciSI GCTCTTC 1 cut(s) 777
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 308
PdmI GAANNNNTTC 2 cut(s) 641, 737
PfeI GAWTC 3 cut(s) 150, 451, 656
PinAI ACCGGT 2 cut(s) 262, 358
PkrI GCNGC 4 cut(s) 201, 309, 765, 825
PleI GAGTC 6 cut(s) 215, 263, 307, 323, 359, 813
PpsI GAGTC 6 cut(s) 215, 263, 307, 323, 359, 813
PpuMI RGGWCCY 1 cut(s) 851
PshBI ATTAAT 1 cut(s) 843
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 5 cut(s) 93, 246, 280, 376, 490
PspGI CCWGG 5 cut(s) 93, 246, 280, 376, 490
PspN4I GGNNCC 7 cut(s) 105, 198, 279, 306, 375, 536, 852
PspOMI GGGCCC 1 cut(s) 534
PspPI GGNCC 4 cut(s) 534, 535, 553, 851
PspPPI RGGWCCY 1 cut(s) 851
PspXI VCTCGAGB 1 cut(s) 758
PstNI CAGNNNCTG 1 cut(s) 808
RseI CAYNNNNRTG 1 cut(s) 792
SalI GTCGAC 2 cut(s) 266, 362
SapI GCTCTTC 1 cut(s) 777
SaqAI TTAA 1 cut(s) 843
SatI GCNGC 4 cut(s) 200, 308, 764, 824
Sau3AI GATC 4 cut(s) 525, 595, 606, 690
Sau96I GGNCC 4 cut(s) 534, 535, 553, 851
SchI GAGTC 6 cut(s) 215, 263, 308, 323, 359, 814
ScrFI CCNGG 6 cut(s) 95, 248, 282, 378, 492, 551
SduI GDGCHC 2 cut(s) 538, 789
SfaNI GCATC 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 758
SinI GGWCC 2 cut(s) 553, 851
SlaI CTCGAG 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 758
SmoI CTYRAG 1 cut(s) 758
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 5 cut(s) 390, 455, 639, 752, 844
SseBI AGGCCT 1 cut(s) 614
SsiI CCGC 5 cut(s) 91, 200, 308, 538, 823
SspMI CTAG 3 cut(s) 521, 545, 858
StuI AGGCCT 1 cut(s) 614
StyD4I CCNGG 6 cut(s) 93, 246, 280, 376, 490, 549
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 737
TaiI ACGT 2 cut(s) 484, 792
TaqI TCGA 5 cut(s) 267, 363, 507, 528, 759
TasI AATT 5 cut(s) 390, 455, 639, 752, 844
TauI GCSGC 3 cut(s) 202, 310, 826
TfiI GAWTC 3 cut(s) 150, 451, 656
Tru1I TTAA 1 cut(s) 843
Tru9I TTAA 1 cut(s) 843
TscAI CASTG 2 cut(s) 750, 799
TseI GCWGC 1 cut(s) 763
TspDTI ATGAA 1 cut(s) 795
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 2 cut(s) 750, 799
VpaK11BI GGWCC 2 cut(s) 553, 851
VspI ATTAAT 1 cut(s) 843
XagI CCTNNNNNAGG 2 cut(s) 98, 489
XapI RAATTY 2 cut(s) 639, 752
XhoI CTCGAG 1 cut(s) 758
XmiI GTMKAC 2 cut(s) 267, 363
XmnI GAANNNNTTC 2 cut(s) 641, 737
XspI CTAG 3 cut(s) 521, 545, 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.