Prupe.6G057400_v2.0.a1

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001145
Physical Location & Seq
Reverse (-)
4014841 .. 4020462
5622 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G057400.1

Sequence Viewer

Length: 1548 bp
ATGAAGCACACGAAGAGGAGAAGGAAGAAGAAGAGCTCGGTAGATCCTAAGCCAGTCTCCAAAGAAGACGAAGGCCAGAAAGTGATCCGCGCGCTTACAGAGGTCTTCTCCTCGGTTTCACTCGACGACGCCGTTTCTGCGTATCGCGAAGCCAACGGTGACCCGAACAAAGCCGCAGAGATTTTGGAGCGAGCTGTGGTTGAAAGCTCCGAAGAGCCGTTCACGAGTTCCACAACGAGTGGCGTTTCTGGATCGGATGCGGGTTCGAGTTGGGGCTCGGGCTCGAGCTCGGGTTCGGGTTCGTCAGAAGGGTTCGAGTCAGGCTGTATTCAGAATTTGGTGAGCGAGAAGGGTTTTAGGGGTAAGCAGAAGAGGGTTGTGGCGGCCGCGGGAACGGTATCGACGGTTTTGGGTAAAGATTACGTGAGCTCGAATCCTAGGAGAGGTTCATGGTCGACGATGACGAAGCTGAAGGGTTTTGGTAATGGAGTTGCTGCTGGGGAAGAAGATGCCGAGCAGTTCCTTTGCTCTATGCTTGGAGATGAGTCTGAGCTAAGCTTGGCTGTTGTTAGAGATGTCCTCTGTCAGTGTGGGTATGATGTTCAAAAGGCCTTGGATGCATTACTTGATGTATCTTCTTCCTCGATTGAACAGTCCAGTAGTAGTTCTAACAATAGTTTGATTTTTAAAGAAGATGGTAGATATCGCATCGATCAGTCTGATACTCTGACAGATAGGGCATCTGATTGCACTTCTCATTCATCTGAAAGCGAGCTCCAGGATAATATATGGTATTCCGGATATACTTGCCGGAATTATGCAAAGGTTCTTGCTAGTTCTGAAGCTCAATCACCCGTCAGCCCAAGAAGTACTCCAGCAGAACTCCCTCAAAAGGTGTTGGAATCTTTGTTTAACATCACCAAGAGTCCTGAATATGAACCAACAGCCATGAATTGGAAGAATGTTGTAAATAAGTTACAATCATTGGCACCCGGGTTTGATGTTTGCACTTCTAGCTCTGCAGAAGCACAGAAGGAGACCTTTGTTAAAGGAGACGAGTATCATGCATTCAGAGGAACTGCAAAGGAGCACTGGGATTCAGTGAGGTCGCATTATCAGAAAGCTGCAATGGCATATTCAAAAGGATCACGGGAATACGCAGGTTACCTCGCTGACCAGGGGAAGGTACAGACAAAGTTGGCACGAGAGGCTGATGAAAGGGCAAGCCAGGATATCTTTAAAGCTAGAAACAAGGGCATAGAAAATATGATAACAATTGATTTGCATGGACAACATGTCAAACAAGCAATGAAGCTTTTAAAAATCCATCTCCTATTCGGAACATATGCACAATCTGTTCAATTCCTCAGGGTCATCACAGGGTGTGGGTCACATGGTGTTGGAAAGTCAAAGCTGAAGCAATCGGTCATCAAACTTATTGAGAATGAAGGTATAAAATGGAGTGAAGAGAATCAGGGAACGGTATTAATCAAGCTAGGTGCACAGAAAGAGTTCAGCTTTCTAGATTCGGAGAGTGATCCTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

516

Amino Acids

56.07

Weight (kDa)

6.09

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1151
AccB1I GGYRCC 1 cut(s) 988
AccB7I CCANNNNNTGG 1 cut(s) 954
AccI GTMKAC 1 cut(s) 455
AccII CGCG 4 cut(s) 90, 92, 147, 389
AccIII TCCGGA 1 cut(s) 797
AciI CCGC 6 cut(s) 88, 174, 260, 383, 387, 389
AclWI GGATC 5 cut(s) 38, 79, 259, 1153, 1532
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 1 cut(s) 334
AcuI CTGAAG 3 cut(s) 491, 861, 1436
AcyI GRCGYC 1 cut(s) 129
AdeI CACNNNGTG 2 cut(s) 1383, 1397
AfaI GTAC 2 cut(s) 871, 1188
AfiI CCNNNNNNNGG 4 cut(s) 443, 892, 954, 1183
AflIII ACRYGT 1 cut(s) 1294
AgsI TTSAA 5 cut(s) 203, 605, 650, 1140, 1361
AhdI GACNNNNNGTC 1 cut(s) 1295
AjnI CCWGG 3 cut(s) 777, 1176, 1227
Alw21I GWGCWC 6 cut(s) 38, 290, 431, 777, 1092, 1504
Alw26I GTCTC 3 cut(s) 61, 1031, 1047
Alw44I GTGCAC 1 cut(s) 1500
AlwI GGATC 5 cut(s) 38, 79, 259, 1153, 1532
Ama87I CYCGRG 4 cut(s) 277, 283, 289, 992
Aor13HI TCCGGA 1 cut(s) 797
AoxI GGCC 3 cut(s) 73, 384, 609
ApaLI GTGCAC 1 cut(s) 1500
ApeKI GCWGC 2 cut(s) 494, 1124
ApoI RAATTY 1 cut(s) 334
AseI ATTAAT 1 cut(s) 1487
Asp700I GAANNNNTTC 1 cut(s) 1511
AspA2I CCTAGG 1 cut(s) 437
AspLEI GCGC 2 cut(s) 92, 94
AsuC2I CCSGG 2 cut(s) 993, 994
AsuHPI GGTGA 4 cut(s) 170, 352, 843, 910
AvaI CYCGRG 4 cut(s) 277, 283, 289, 992
AvrII CCTAGG 1 cut(s) 437
AxyI CCTNAGG 1 cut(s) 1367
BaeGI GKGCMC 1 cut(s) 1504
BanI GGYRCC 1 cut(s) 988
BanII GRGCYC 6 cut(s) 38, 278, 284, 290, 431, 777
BauI CACGAG 2 cut(s) 223, 1203
BbsI GAAGAC 2 cut(s) 72, 97
Bbv12I GWGCWC 6 cut(s) 38, 290, 431, 777, 1092, 1504
BbvI GCAGC 2 cut(s) 481, 1111
BccI CCATC 2 cut(s) 689, 1335
BceAI ACGGC 2 cut(s) 116, 202
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 3 cut(s) 779, 1178, 1229
BcnI CCSGG 2 cut(s) 993, 994
BcoDI GTCTC 3 cut(s) 61, 1031, 1047
BfaI CTAG 6 cut(s) 438, 834, 1014, 1245, 1496, 1523
BfmI CTRYAG 1 cut(s) 1020
BfuAI ACCTGC 1 cut(s) 1151
BisI GCNGC 5 cut(s) 174, 384, 387, 495, 1125
BlnI CCTAGG 1 cut(s) 437
BlpI GCTNAGC 1 cut(s) 554
BlsI GCNGC 5 cut(s) 175, 385, 388, 496, 1126
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 5 cut(s) 779, 993, 994, 1178, 1229
BmeRI GACNNNNNGTC 1 cut(s) 1295
BmeT110I CYCGRG 4 cut(s) 277, 283, 289, 992
BmiI GGNNCC 1 cut(s) 990
BmrFI CCNGG 5 cut(s) 779, 993, 994, 1178, 1229
BmrI ACTGGG 1 cut(s) 1102
BmsI GCATC 5 cut(s) 247, 499, 607, 717, 749
BmuI ACTGGG 1 cut(s) 1102
BpiI GAAGAC 2 cut(s) 72, 97
BplI GAGNNNNNCTC 4 cut(s) 92, 124, 564, 596
BpmI CTGGAG 2 cut(s) 761, 858
Bpu10I CCTNAGC 1 cut(s) 48
Bpu1102I GCTNAGC 1 cut(s) 554
BpuMI CCSGG 2 cut(s) 993, 994
Bsa29I ATCGAT 1 cut(s) 711
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 6 cut(s) 111, 387, 437, 612, 992, 1177
BsaWI WCCGGW 1 cut(s) 797
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 892, 954, 1183
Bse1I ACTGG 3 cut(s) 53, 657, 1097
Bse21I CCTNAGG 1 cut(s) 1367
Bse3DI GCAATG 2 cut(s) 1134, 1314
BseAI TCCGGA 1 cut(s) 797
BseBI CCWGG 3 cut(s) 779, 1178, 1229
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 6 cut(s) 111, 387, 437, 612, 992, 1177
BseGI GGATG 2 cut(s) 262, 622
BseLI CCNNNNNNNGG 4 cut(s) 443, 892, 954, 1183
BseMI GCAATG 2 cut(s) 1134, 1314
BseMII CTCAG 3 cut(s) 540, 1381, 1533
BseNI ACTGG 3 cut(s) 53, 657, 1097
BsePI GCGCGC 1 cut(s) 90
BseRI GAGGAG 2 cut(s) 31, 100
BseSI GKGCMC 1 cut(s) 1504
BseX3I CGGCCG 1 cut(s) 384
BseXI GCAGC 2 cut(s) 481, 1111
BseYI CCCAGC 1 cut(s) 497
Bsh1236I CGCG 4 cut(s) 90, 92, 147, 389
Bsh1285I CGRYCG 1 cut(s) 387
BshFI GGCC 3 cut(s) 75, 386, 611
BshNI GGYRCC 1 cut(s) 988
BshVI ATCGAT 1 cut(s) 711
BsiEI CGRYCG 1 cut(s) 387
BsiHKAI GWGCWC 6 cut(s) 38, 290, 431, 777, 1092, 1504
BsiHKCI CYCGRG 4 cut(s) 277, 283, 289, 992
BsiSI CCGG 3 cut(s) 798, 811, 993
BslI CCNNNNNNNGG 4 cut(s) 443, 892, 954, 1183
BsmAI GTCTC 3 cut(s) 61, 1031, 1047
BsmBI CGTCTC 1 cut(s) 1047
BsmI GAATGC 1 cut(s) 1067
BsnI GGCC 3 cut(s) 75, 386, 611
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 4 cut(s) 277, 283, 289, 992
Bsp1286I GDGCHC 8 cut(s) 38, 278, 284, 290, 431, 777, 1092, 1504
Bsp13I TCCGGA 1 cut(s) 797
Bsp143I GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
Bsp1720I GCTNAGC 1 cut(s) 554
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 6 cut(s) 88, 174, 260, 383, 387, 389
BspANI GGCC 3 cut(s) 75, 386, 611
BspCNI CTCAG 3 cut(s) 541, 1380, 1534
BspDI ATCGAT 1 cut(s) 711
BspEI TCCGGA 1 cut(s) 797
BspFNI CGCG 4 cut(s) 90, 92, 147, 389
BspLI GGNNCC 1 cut(s) 990
BspMAI CTGCAG 1 cut(s) 1024
BspMI ACCTGC 1 cut(s) 1151
BspPI GGATC 5 cut(s) 38, 79, 259, 1153, 1532
BspQI GCTCTTC 2 cut(s) 26, 207
BspT107I GGYRCC 1 cut(s) 988
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 2 cut(s) 1134, 1314
BsrI ACTGG 3 cut(s) 53, 657, 1097
BssECI CCNNGG 6 cut(s) 111, 387, 437, 612, 992, 1177
BssHII GCGCGC 1 cut(s) 90
BssMI GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 2 cut(s) 223, 1203
BssT1I CCWWGG 2 cut(s) 437, 612
Bst2BI CACGAG 2 cut(s) 223, 1203
Bst2UI CCWGG 3 cut(s) 779, 1178, 1229
Bst4CI ACNGT 5 cut(s) 158, 397, 406, 654, 1483
Bst6I CTCTTC 5 cut(s) 8, 26, 207, 365, 1461
BstACI GRCGYC 1 cut(s) 129
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 4 cut(s) 92, 192, 773, 1225
BstDEI CTNAG 5 cut(s) 48, 549, 554, 1367, 1542
BstDSI CCRYGG 1 cut(s) 387
BstEII GGTNACC 2 cut(s) 158, 1163
BstENI CCTNNNNNAGG 1 cut(s) 441
BstF5I GGATG 2 cut(s) 262, 622
BstFNI CGCG 4 cut(s) 90, 92, 147, 389
BstHHI GCGC 2 cut(s) 92, 94
BstKTI GATC 6 cut(s) 46, 87, 254, 715, 1148, 1540
BstMAI GTCTC 3 cut(s) 61, 1031, 1047
BstMBI GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
BstMCI CGRYCG 1 cut(s) 387
BstMWI GCNNNNNNNGC 5 cut(s) 137, 617, 1014, 1130, 1208
BstNI CCWGG 3 cut(s) 779, 1178, 1229
BstNSI RCATGY 1 cut(s) 1298
BstPI GGTNACC 2 cut(s) 158, 1163
BstSCI CCNGG 5 cut(s) 777, 991, 992, 1176, 1227
BstSFI CTRYAG 1 cut(s) 1020
BstSLI GKGCMC 1 cut(s) 1504
BstUI CGCG 4 cut(s) 90, 92, 147, 389
BstV1I GCAGC 2 cut(s) 481, 1111
BstV2I GAAGAC 2 cut(s) 72, 97
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BstZI CGGCCG 1 cut(s) 384
Bsu15I ATCGAT 1 cut(s) 711
Bsu36I CCTNAGG 1 cut(s) 1367
BsuRI GGCC 3 cut(s) 75, 386, 611
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 262, 622
BtsIMutI CAGTG 3 cut(s) 593, 1090, 1107
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 1 cut(s) 1151
Cac8I GCNNGC 4 cut(s) 92, 192, 773, 1225
CciNI GCGGCCGC 1 cut(s) 384
CfoI GCGC 2 cut(s) 92, 94
Cfr42I CCGCGG 1 cut(s) 390
Cfr9I CCCGGG 1 cut(s) 992
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 137
Csp6I GTAC 2 cut(s) 870, 1187
CviAII CATG 6 cut(s) 450, 949, 1064, 1286, 1295, 1394
CviQI GTAC 2 cut(s) 870, 1187
DdeI CTNAG 5 cut(s) 48, 549, 554, 1367, 1542
DpnI GATC 6 cut(s) 45, 86, 253, 714, 1147, 1539
DpnII GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
DraI TTTAAA 3 cut(s) 688, 1240, 1320
DraIII CACNNNGTG 2 cut(s) 1383, 1397
DriI GACNNNNNGTC 1 cut(s) 1295
EaeI YGGCCR 1 cut(s) 384
EagI CGGCCG 1 cut(s) 384
Eam1104I CTCTTC 5 cut(s) 8, 26, 207, 365, 1461
Eam1105I GACNNNNNGTC 1 cut(s) 1295
EarI CTCTTC 5 cut(s) 8, 26, 207, 365, 1461
Ecl136II GAGCTC 4 cut(s) 36, 288, 429, 775
EclXI CGGCCG 1 cut(s) 384
Eco130I CCWWGG 2 cut(s) 437, 612
Eco147I AGGCCT 1 cut(s) 611
Eco24I GRGCYC 6 cut(s) 38, 278, 284, 290, 431, 777
Eco31I GGTCTC 1 cut(s) 1031
Eco32I GATATC 2 cut(s) 704, 1234
Eco52I CGGCCG 1 cut(s) 384
Eco53kI GAGCTC 4 cut(s) 36, 288, 429, 775
Eco57I CTGAAG 3 cut(s) 491, 861, 1436
Eco81I CCTNAGG 1 cut(s) 1367
Eco88I CYCGRG 4 cut(s) 277, 283, 289, 992
Eco91I GGTNACC 2 cut(s) 158, 1163
EcoICRI GAGCTC 4 cut(s) 36, 288, 429, 775
EcoNI CCTNNNNNAGG 1 cut(s) 441
EcoO65I GGTNACC 2 cut(s) 158, 1163
EcoRII CCWGG 3 cut(s) 777, 1176, 1227
EcoRV GATATC 2 cut(s) 704, 1234
EcoT14I CCWWGG 2 cut(s) 437, 612
EcoT22I ATGCAT 2 cut(s) 622, 1069
EcoT38I GRGCYC 6 cut(s) 38, 278, 284, 290, 431, 777
ErhI CCWWGG 2 cut(s) 437, 612
Esp3I CGTCTC 1 cut(s) 1047
FaeI CATG 6 cut(s) 453, 952, 1067, 1289, 1298, 1397
FalI AAGNNNNNCTT 2 cut(s) 1025, 1057
FatI CATG 6 cut(s) 449, 948, 1063, 1285, 1294, 1393
FauI CCCGC 2 cut(s) 253, 382
FauNDI CATATG 1 cut(s) 1345
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 5 cut(s) 174, 384, 387, 495, 1125
FokI GGATG 2 cut(s) 269, 629
FriOI GRGCYC 6 cut(s) 38, 278, 284, 290, 431, 777
Fsp4HI GCNGC 5 cut(s) 174, 384, 387, 495, 1125
FspBI CTAG 6 cut(s) 438, 834, 1014, 1245, 1496, 1523
GlaI GCGC 2 cut(s) 91, 93
GluI GCNGC 5 cut(s) 174, 384, 387, 495, 1125
GsaI CCCAGC 1 cut(s) 501
GsuI CTGGAG 2 cut(s) 761, 858
HaeIII GGCC 3 cut(s) 75, 386, 611
HapII CCGG 3 cut(s) 798, 811, 993
HgaI GACGC 1 cut(s) 137
HhaI GCGC 2 cut(s) 92, 94
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 6 cut(s) 453, 952, 1067, 1289, 1298, 1397
Hin6I GCGC 2 cut(s) 90, 92
HinP1I GCGC 2 cut(s) 90, 92
HincII GTYRAC 1 cut(s) 456
HindII GTYRAC 1 cut(s) 456
HindIII AAGCTT 2 cut(s) 556, 1313
HinfI GANTC 8 cut(s) 317, 433, 545, 902, 925, 1097, 1471, 1526
HpaII CCGG 3 cut(s) 798, 811, 993
HphI GGTGA 4 cut(s) 170, 352, 843, 910
Hpy166II GTNNAC 3 cut(s) 222, 456, 1502
Hpy188III TCNNGA 7 cut(s) 146, 223, 249, 798, 929, 1523, 1541
Hpy8I GTNNAC 3 cut(s) 222, 456, 1502
Hpy99I CGWCG 4 cut(s) 128, 131, 406, 460
HpyAV CCTTC 8 cut(s) 15, 65, 302, 343, 466, 1027, 1177, 1442
HpyCH4III ACNGT 5 cut(s) 158, 397, 406, 654, 1483
HpyCH4IV ACGT 1 cut(s) 423
HpyF10VI GCNNNNNNNGC 5 cut(s) 137, 617, 1014, 1130, 1208
HpyF3I CTNAG 5 cut(s) 48, 549, 554, 1367, 1542
HpySE526I ACGT 1 cut(s) 423
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 6 cut(s) 453, 952, 1067, 1289, 1298, 1397
HspAI GCGC 2 cut(s) 90, 92
Kpn2I TCCGGA 1 cut(s) 797
KspI CCGCGG 1 cut(s) 390
Kzo9I GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
LguI GCTCTTC 2 cut(s) 26, 207
LmnI GCTCC 4 cut(s) 187, 212, 780, 1087
Lsp1109I GCAGC 2 cut(s) 481, 1111
LweI GCATC 5 cut(s) 247, 499, 607, 717, 749
MaeI CTAG 6 cut(s) 438, 834, 1014, 1245, 1496, 1523
MaeII ACGT 1 cut(s) 423
MaeIII GTNAC 4 cut(s) 158, 975, 1163, 1389
MalI GATC 6 cut(s) 45, 86, 253, 714, 1147, 1539
MboI GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
MfeI CAATTG 1 cut(s) 1275
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 8 cut(s) 38, 278, 284, 290, 431, 777, 1092, 1504
MluCI AATT 5 cut(s) 334, 814, 952, 1275, 1361
MlyI GAGTC 3 cut(s) 326, 554, 934
MmeI TCCRAC 2 cut(s) 879, 1381
Mph1103I ATGCAT 2 cut(s) 622, 1069
MroI TCCGGA 1 cut(s) 797
MroXI GAANNNNTTC 1 cut(s) 1511
MseI TTAA 6 cut(s) 687, 912, 1047, 1239, 1319, 1487
MspA1I CMGCKG 1 cut(s) 389
MspI CCGG 3 cut(s) 798, 811, 993
MspR9I CCNGG 5 cut(s) 779, 993, 994, 1178, 1229
MunI CAATTG 1 cut(s) 1275
Mva1269I GAATGC 1 cut(s) 1067
MvaI CCWGG 3 cut(s) 779, 1178, 1229
MvnI CGCG 4 cut(s) 90, 92, 147, 389
MwoI GCNNNNNNNGC 5 cut(s) 137, 617, 1014, 1130, 1208
NciI CCSGG 2 cut(s) 993, 994
NdeI CATATG 1 cut(s) 1345
NdeII GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
NlaIII CATG 6 cut(s) 453, 952, 1067, 1289, 1298, 1397
NlaIV GGNNCC 1 cut(s) 990
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 2 cut(s) 158, 1389
NotI GCGGCCGC 1 cut(s) 384
NruI TCGCGA 1 cut(s) 147
NsiI ATGCAT 2 cut(s) 622, 1069
NspI RCATGY 1 cut(s) 1298
PaeR7I CTCGAG 1 cut(s) 283
PauI GCGCGC 1 cut(s) 90
PceI AGGCCT 1 cut(s) 611
PciI ACATGT 1 cut(s) 1294
PciSI GCTCTTC 2 cut(s) 26, 207
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 461
PctI GAATGC 1 cut(s) 1067
PdmI GAANNNNTTC 1 cut(s) 1511
PfeI GAWTC 5 cut(s) 433, 902, 1097, 1471, 1526
PflMI CCANNNNNTGG 1 cut(s) 954
PfoI TCCNGGA 1 cut(s) 777
PkrI GCNGC 5 cut(s) 175, 385, 388, 496, 1126
PleI GAGTC 3 cut(s) 325, 553, 933
PpsI GAGTC 3 cut(s) 325, 553, 933
Ppu21I YACGTR 1 cut(s) 424
PscI ACATGT 1 cut(s) 1294
PshBI ATTAAT 1 cut(s) 1487
Psp124BI GAGCTC 4 cut(s) 38, 290, 431, 777
Psp6I CCWGG 3 cut(s) 777, 1176, 1227
PspEI GGTNACC 2 cut(s) 158, 1163
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 3 cut(s) 777, 1176, 1227
PspN4I GGNNCC 1 cut(s) 990
PspXI VCTCGAGB 1 cut(s) 283
PstI CTGCAG 1 cut(s) 1024
PsuI RGATCY 1 cut(s) 43
PteI GCGCGC 1 cut(s) 90
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 2 cut(s) 871, 1188
RsaNI GTAC 2 cut(s) 870, 1187
SacI GAGCTC 4 cut(s) 38, 290, 431, 777
SacII CCGCGG 1 cut(s) 390
SalI GTCGAC 1 cut(s) 454
SapI GCTCTTC 2 cut(s) 26, 207
SaqAI TTAA 6 cut(s) 687, 912, 1047, 1239, 1319, 1487
SatI GCNGC 5 cut(s) 174, 384, 387, 495, 1125
Sau3AI GATC 6 cut(s) 43, 84, 251, 712, 1145, 1537
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 3 cut(s) 326, 554, 934
ScrFI CCNGG 5 cut(s) 779, 993, 994, 1178, 1229
SduI GDGCHC 8 cut(s) 38, 278, 284, 290, 431, 777, 1092, 1504
SfaNI GCATC 5 cut(s) 247, 499, 607, 717, 749
SfcI CTRYAG 1 cut(s) 1020
Sfr274I CTCGAG 1 cut(s) 283
Sfr303I CCGCGG 1 cut(s) 390
SgrBI CCGCGG 1 cut(s) 390
SlaI CTCGAG 1 cut(s) 283
SmaI CCCGGG 1 cut(s) 994
SmlI CTYRAG 1 cut(s) 283
SmoI CTYRAG 1 cut(s) 283
Sse9I AATT 5 cut(s) 334, 814, 952, 1275, 1361
SseBI AGGCCT 1 cut(s) 611
SsiI CCGC 6 cut(s) 88, 174, 260, 383, 387, 389
SspMI CTAG 6 cut(s) 438, 834, 1014, 1245, 1496, 1523
SstI GAGCTC 4 cut(s) 38, 290, 431, 777
StuI AGGCCT 1 cut(s) 611
StyD4I CCNGG 5 cut(s) 777, 991, 992, 1176, 1227
StyI CCWWGG 2 cut(s) 437, 612
TaaI ACNGT 5 cut(s) 158, 397, 406, 654, 1483
TaiI ACGT 1 cut(s) 426
TaqI TCGA 9 cut(s) 123, 266, 284, 315, 401, 431, 455, 644, 711
TaqII GACCGA 1 cut(s) 1414
TasI AATT 5 cut(s) 334, 814, 952, 1275, 1361
TatI WGTACW 1 cut(s) 869
TauI GCSGC 3 cut(s) 176, 386, 389
TfiI GAWTC 5 cut(s) 433, 902, 1097, 1471, 1526
Tru1I TTAA 6 cut(s) 687, 912, 1047, 1239, 1319, 1487
Tru9I TTAA 6 cut(s) 687, 912, 1047, 1239, 1319, 1487
TscAI CASTG 3 cut(s) 593, 1097, 1107
TseFI GTSAC 2 cut(s) 158, 1389
TseI GCWGC 2 cut(s) 494, 1124
Tsp45I GTSAC 2 cut(s) 158, 1389
TspDTI ATGAA 8 cut(s) 17, 438, 750, 951, 965, 1230, 1325, 1461
TspMI CCCGGG 1 cut(s) 992
TspRI CASTG 3 cut(s) 593, 1097, 1107
Van91I CCANNNNNTGG 1 cut(s) 954
VneI GTGCAC 1 cut(s) 1500
VspI ATTAAT 1 cut(s) 1487
XagI CCTNNNNNAGG 1 cut(s) 441
XapI RAATTY 1 cut(s) 334
XbaI TCTAGA 1 cut(s) 1522
XceI RCATGY 1 cut(s) 1298
XhoI CTCGAG 1 cut(s) 283
XmaI CCCGGG 1 cut(s) 992
XmaJI CCTAGG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 455
XmnI GAANNNNTTC 1 cut(s) 1511
XspI CTAG 6 cut(s) 438, 834, 1014, 1245, 1496, 1523
ZrmI AGTACT 1 cut(s) 871
Zsp2I ATGCAT 2 cut(s) 622, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.