Rw7G017800

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001145
Physical Location & Seq
Reverse (-)
18883077 .. 18883940
864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G017800.1

Sequence Viewer

Length: 864 bp
ATGTTTGGAGACGAGTCGGAGATCATAAGCTTGGGTGTGGTTAAAGATGTCCTTGGTCAGTGTGGGTATGAGGTTGAAAAGGCCTTGGATACCTTGCTCGAGTTATCTTCTTCCTCAAGTAAGAGACAGTGCTCTTCAGAATATACCAGCAAGAATTATGCCCAGGTTCTTGCTGGTCCTTCTCAAGCTCATAGGAGTACGAGTGCAAATCTGTCTCAAATGGTGTTGGAATCTTTGTTCAACAACTCAAAGAGAAGTCGTCAGAATAAAGAACCAAGAACTATGAGTTCTTGGAAGTATAGTGTCAAGGAATTGCAAGATTCTTTGGGACCCGAGTTTGATGTTGCTAAGGGAGCTAAATATGATGCATACAGAAGTACTGCAAAGGAGCATTGGAATTCAGTGAAGTCCTACCATCAGAAAGCTGCGACTGCGTATTCAAACGGATCACGGGCATATGCAGTTTACCTATCCGAGCAGGCCAAGGCAAAGGCAAAAATGGCACGAGAGGAGGACAAGAAGGCAAGCCTGGAGATATTAAAAGCTAAAAACAAGGACAAGGGCATAAAAAATGATGTGATGACAATTGATTTGCATGGGCAGCATGTCAACGAAGCAATGAAGCTTTTGAAGATGCACATTCAGTATGGATCCAATACACAATCGCTTCGATTTCTCAGGGTTATTACAGGACATGGTGCAGGGAAGTCTATTGTGAAACAATCTGTCATCAAATTTTTCCAGAATGAAGGTATTAAATGGAGTGAAGAGAATCCAGGAGCGGTGTTAATCAAGCTAGGTTCACACAGAGACTACTTCAGATATATGGACAGGGATATACGTAGTATATGTTCAGTCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

287

Amino Acids

32.21

Weight (kDa)

9.41

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 121 - 184 5.4e-13 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 782
AciI CCGC 1 cut(s) 782
AclWI GGATC 3 cut(s) 454, 645, 658
AcsI RAATTY 2 cut(s) 397, 734
AcuI CTGAAG 2 cut(s) 120, 802
AfaI GTAC 2 cut(s) 199, 379
AgsI TTSAA 4 cut(s) 77, 241, 441, 631
AjnI CCWGG 3 cut(s) 162, 528, 775
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
AluBI AGCT 8 cut(s) 30, 188, 356, 425, 545, 625, 796, 861
AluI AGCT 8 cut(s) 30, 188, 356, 425, 545, 625, 796, 861
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 4 cut(s) 3, 118, 219, 804
AlwI GGATC 3 cut(s) 454, 645, 658
Ama87I CYCGRG 2 cut(s) 98, 332
AoxI GGCC 2 cut(s) 81, 480
ApeKI GCWGC 2 cut(s) 425, 601
ApoI RAATTY 2 cut(s) 397, 734
ArsI GACNNNNNNTTYG 2 cut(s) 574, 606
AspS9I GGNCC 2 cut(s) 176, 329
AvaI CYCGRG 2 cut(s) 98, 332
AvaII GGWCC 2 cut(s) 176, 329
BamHI GGATCC 1 cut(s) 650
BauI CACGAG 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 412, 613
BccI CCATC 1 cut(s) 423
BciT130I CCWGG 3 cut(s) 164, 530, 777
BciVI GTATCC 1 cut(s) 82
BcoDI GTCTC 4 cut(s) 3, 118, 219, 804
BfaI CTAG 1 cut(s) 797
BfuI GTATCC 1 cut(s) 82
BisI GCNGC 2 cut(s) 426, 602
BlsI GCNGC 2 cut(s) 427, 603
BmcAI AGTACT 1 cut(s) 379
Bme1390I CCNGG 3 cut(s) 164, 530, 777
Bme18I GGWCC 2 cut(s) 176, 329
BmeT110I CYCGRG 2 cut(s) 98, 332
BmgT120I GGNCC 2 cut(s) 176, 329
BmiI GGNNCC 3 cut(s) 330, 331, 652
BmrFI CCNGG 3 cut(s) 164, 530, 777
BmsI GCATC 2 cut(s) 355, 624
BpmI CTGGAG 1 cut(s) 551
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 2 cut(s) 100, 168
BsaAI YACGTR 1 cut(s) 842
BsaJI CCNNGG 4 cut(s) 52, 84, 162, 483
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bse3DI GCAATG 1 cut(s) 624
BseBI CCWGG 3 cut(s) 164, 530, 777
BseDI CCNNGG 4 cut(s) 52, 84, 162, 483
BseMI GCAATG 1 cut(s) 624
BseMII CTCAG 1 cut(s) 691
BseRI GAGGAG 1 cut(s) 524
BseXI GCAGC 2 cut(s) 412, 613
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 2 cut(s) 83, 482
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 2 cut(s) 98, 332
BslFI GGGAC 1 cut(s) 342
BsmAI GTCTC 4 cut(s) 3, 118, 219, 804
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 2 cut(s) 83, 482
BsoBI CYCGRG 2 cut(s) 98, 332
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 21, 446, 650
BspACI CCGC 1 cut(s) 782
BspANI GGCC 2 cut(s) 83, 482
BspCNI CTCAG 1 cut(s) 690
BspLI GGNNCC 3 cut(s) 330, 331, 652
BspPI GGATC 3 cut(s) 454, 645, 658
BspQI GCTCTTC 1 cut(s) 139
BsrBI CCGCTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 624
BssECI CCNNGG 4 cut(s) 52, 84, 162, 483
BssMI GATC 3 cut(s) 21, 446, 650
BssSI CACGAG 1 cut(s) 504
BssT1I CCWWGG 3 cut(s) 52, 84, 483
Bst2BI CACGAG 1 cut(s) 504
Bst2UI CCWGG 3 cut(s) 164, 530, 777
Bst4CI ACNGT 1 cut(s) 129
Bst6I CTCTTC 2 cut(s) 139, 762
BstBAI YACGTR 1 cut(s) 842
BstC8I GCNNGC 2 cut(s) 480, 526
BstDEI CTNAG 2 cut(s) 348, 677
BstKTI GATC 3 cut(s) 24, 449, 653
BstMAI GTCTC 4 cut(s) 3, 118, 219, 804
BstMBI GATC 3 cut(s) 21, 446, 650
BstMWI GCNNNNNNNGC 4 cut(s) 353, 431, 500, 601
BstNI CCWGG 3 cut(s) 164, 530, 777
BstNSI RCATGY 1 cut(s) 608
BstSCI CCNGG 3 cut(s) 162, 528, 775
BstSNI TACGTA 1 cut(s) 842
BstV1I GCAGC 2 cut(s) 412, 613
BstX2I RGATCY 1 cut(s) 650
BstYI RGATCY 1 cut(s) 650
BsuI GTATCC 1 cut(s) 82
BsuRI GGCC 2 cut(s) 83, 482
BtsIMutI CAGTG 3 cut(s) 65, 134, 408
Cac8I GCNNGC 2 cut(s) 480, 526
Cfr13I GGNCC 2 cut(s) 176, 329
Csp6I GTAC 2 cut(s) 198, 378
CviAII CATG 3 cut(s) 596, 605, 695
CviQI GTAC 2 cut(s) 198, 378
DdeI CTNAG 2 cut(s) 348, 677
DpnI GATC 3 cut(s) 23, 448, 652
DpnII GATC 3 cut(s) 21, 446, 650
Eam1104I CTCTTC 2 cut(s) 139, 762
EarI CTCTTC 2 cut(s) 139, 762
Eco105I TACGTA 1 cut(s) 842
Eco130I CCWWGG 3 cut(s) 52, 84, 483
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 176, 329
Eco57I CTGAAG 2 cut(s) 120, 802
Eco88I CYCGRG 2 cut(s) 98, 332
EcoO109I RGGNCCY 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 397
EcoRII CCWGG 3 cut(s) 162, 528, 775
EcoT14I CCWWGG 3 cut(s) 52, 84, 483
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 3 cut(s) 52, 84, 483
Esp3I CGTCTC 1 cut(s) 3
FaeI CATG 3 cut(s) 599, 608, 698
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FaqI GGGAC 1 cut(s) 342
FatI CATG 3 cut(s) 595, 604, 694
FauNDI CATATG 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 426, 602
Fsp4HI GCNGC 2 cut(s) 426, 602
FspBI CTAG 1 cut(s) 797
GluI GCNGC 2 cut(s) 426, 602
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 2 cut(s) 83, 482
Hin1II CATG 3 cut(s) 599, 608, 698
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HindIII AAGCTT 2 cut(s) 28, 623
HinfI GANTC 4 cut(s) 14, 230, 320, 772
Hpy166II GTNNAC 3 cut(s) 466, 610, 803
Hpy188I TCNGA 6 cut(s) 19, 139, 264, 420, 475, 821
Hpy188III TCNNGA 1 cut(s) 742
Hpy8I GTNNAC 3 cut(s) 466, 610, 803
HpyAV CCTTC 3 cut(s) 189, 514, 743
HpyCH4III ACNGT 1 cut(s) 129
HpyCH4IV ACGT 1 cut(s) 841
HpyCH4V TGCA 8 cut(s) 206, 316, 368, 383, 461, 595, 637, 701
HpyF10VI GCNNNNNNNGC 4 cut(s) 353, 431, 500, 601
HpyF3I CTNAG 2 cut(s) 348, 677
HpySE526I ACGT 1 cut(s) 841
Hsp92II CATG 3 cut(s) 599, 608, 698
KflI GGGWCCC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 21, 446, 650
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 3 cut(s) 353, 388, 779
Lsp1109I GCAGC 2 cut(s) 412, 613
LweI GCATC 2 cut(s) 355, 624
MaeI CTAG 1 cut(s) 797
MaeII ACGT 1 cut(s) 841
MalI GATC 3 cut(s) 23, 448, 652
MbiI CCGCTC 1 cut(s) 782
MboI GATC 3 cut(s) 21, 446, 650
MboII GAAGA 5 cut(s) 99, 102, 126, 643, 779
MfeI CAATTG 1 cut(s) 585
MflI RGATCY 1 cut(s) 650
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 5 cut(s) 154, 311, 397, 585, 734
MlyI GAGTC 1 cut(s) 23
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 4 cut(s) 64, 124, 502, 505
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 4 cut(s) 42, 539, 756, 788
MspR9I CCNGG 3 cut(s) 164, 530, 777
MunI CAATTG 1 cut(s) 585
MvaI CCWGG 3 cut(s) 164, 530, 777
MwoI GCNNNNNNNGC 4 cut(s) 353, 431, 500, 601
NdeI CATATG 1 cut(s) 457
NdeII GATC 3 cut(s) 21, 446, 650
NlaIII CATG 3 cut(s) 599, 608, 698
NlaIV GGNNCC 3 cut(s) 330, 331, 652
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 608
PaeR7I CTCGAG 1 cut(s) 98
PceI AGGCCT 1 cut(s) 83
PciSI GCTCTTC 1 cut(s) 139
PfeI GAWTC 3 cut(s) 230, 320, 772
PfoI TCCNGGA 1 cut(s) 775
PkrI GCNGC 2 cut(s) 427, 603
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Ppu21I YACGTR 1 cut(s) 842
PpuMI RGGWCCY 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 3 cut(s) 162, 528, 775
PspGI CCWGG 3 cut(s) 162, 528, 775
PspN4I GGNNCC 3 cut(s) 330, 331, 652
PspPI GGNCC 2 cut(s) 176, 329
PspPPI RGGWCCY 1 cut(s) 329
PspXI VCTCGAGB 1 cut(s) 98
PsrI GAACNNNNNNTAC 1 cut(s) 835
PsuI RGATCY 1 cut(s) 650
RsaI GTAC 2 cut(s) 199, 379
RsaNI GTAC 2 cut(s) 198, 378
SapI GCTCTTC 1 cut(s) 139
SaqAI TTAA 4 cut(s) 42, 539, 756, 788
SatI GCNGC 2 cut(s) 426, 602
Sau3AI GATC 3 cut(s) 21, 446, 650
Sau96I GGNCC 2 cut(s) 176, 329
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 1 cut(s) 23
ScrFI CCNGG 3 cut(s) 164, 530, 777
SduI GDGCHC 1 cut(s) 134
SfaNI GCATC 2 cut(s) 355, 624
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 2 cut(s) 176, 329
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 98, 115, 183
SmoI CTYRAG 3 cut(s) 98, 115, 183
SnaBI TACGTA 1 cut(s) 842
Sse9I AATT 5 cut(s) 154, 311, 397, 585, 734
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 1 cut(s) 782
SspMI CTAG 1 cut(s) 797
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 3 cut(s) 162, 528, 775
StyI CCWWGG 3 cut(s) 52, 84, 483
TaaI ACNGT 1 cut(s) 129
TaiI ACGT 1 cut(s) 844
TaqI TCGA 2 cut(s) 99, 670
TasI AATT 5 cut(s) 154, 311, 397, 585, 734
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 3 cut(s) 230, 320, 772
Tru1I TTAA 4 cut(s) 42, 539, 756, 788
Tru9I TTAA 4 cut(s) 42, 539, 756, 788
TscAI CASTG 3 cut(s) 65, 134, 408
TseI GCWGC 2 cut(s) 425, 601
TspDTI ATGAA 2 cut(s) 635, 762
TspGWI ACGGA 1 cut(s) 459
TspRI CASTG 3 cut(s) 65, 134, 408
VpaK11BI GGWCC 2 cut(s) 176, 329
XapI RAATTY 2 cut(s) 397, 734
XceI RCATGY 1 cut(s) 608
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XhoI CTCGAG 1 cut(s) 98
XspI CTAG 1 cut(s) 797
ZrmI AGTACT 1 cut(s) 379
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.