MD03G1070600.v1.1

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001145
Physical Location & Seq
Reverse (-)
5765306 .. 5771379
6074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1070600.v1.1.491

Sequence Viewer

Length: 1506 bp
ATGAAGCACACAAGGAGGATAAAGAAGAGGAAGAGCTCGGCGAATCCGACGCGGGTCGCCAAGGAAGACGAAGAACAGAAGGTAATCCGCGCACTTACGGAAGTTTTCTCGTCGGTATCGCTTGACGATGCCATTTATGCGTATCGCGAAGCCGATGGCGACGCGAACAAGGCCGCGGAGATTTTGGAACGGGCGGTGGCTGAAACCGAAAGTTCTGAAGAGCCGTTCACGAGCTCTACAACGAGTGGTGGATCGGACGCGGGCTCGAGCTCGGGTTCGGGTTCGTCGGAAGGGTTCGAGTCCGGTTGTATTCAGAATTTGGTGAGCGAGAAGGGTTTTAGGGGTAAGCAGAAGAGGGTTGTGGCGGCCGCCGGAACGGTATCGACGGTTTTGGGTAAGGAGTACGTGAGCTCGATTCCCAGGAGGGGTCCAACGTTGTCAGAGATGTCGAAGCGGAAGGGTTTTGGTAATGGAGGTGCTGCAGGGGAAGAGGAAGCTGAGCAGTTTCTTTGTTCTATGCTTGGAGATGAGTCTGATCTGAGCTTGGCGGTTGTTAGAGACGTCCTTTGTCAGTGTGAGTATGATGTTGAAAAGGCCTTGGATGCATTGCTTGAGGTTTCTTCTTCGTATGAACAGTCCTGTAGTAGGTCTGACTATAGTATGTTTTTTAAGGAAGATAGTAGACACCCCTTCGAACAGTCTGATGCTCTGACAGATAGGGCATCTGATTGCACTTCTCATTCATCTGAATATGAGCAGGATAATATATGGAATTATGGGAAGGTACTCACTATTTCTGAAGCTCATTCTCCTGTTAGCCCCAAAAGTACTCCGGCAGAGCTCCCTCAAAAGGTGTTGGAATCTTTGTTTAACATCTCCAAGAGTCCCGAATATGAACCAAAAACCATGAATTGGAAGAATGTTGCAACTAAATTGCAGTCATTGGCACCTGGGTATAATGTTTGCACTTCTAGTGCAGCAGAAGCACAGCAGGACACTTATGTTAAAGGAGATGAATATCATGCATTCAGAGGAACTGCAAAGGAGCACTGGGATTCAGTGAGGTCCTATTATCAGAAAGCTGCAACGGCATATTCAAGAGGAGCACGGGAACATGCAGGTTACCTCGCTGAGCAGGGGAGGATACAAACTAAATTGGCACAAGTGGCTGACGAAAGGGCAAGCCAGGAAATCTTTAAAGCTAGAAACAAGGGCATAGAAAATGTGATAACAATTGATTTGCATGGGCAACATGTCAAACAAGCCATGAAACTTTTAAAAATCCATCTACTGTTTGGGACGTATGCACAATCGGTTCAATTCCTCAGGGTTATCACAGGGTACGGGTCACATGATTATGGAAAGTCAAAGCTGAAGCAATCGGTCATTGAACTTGTTGAGAATGAAGGTATTCAATGGAGCGAAGAGAATCGAGGAGCAGTATTAATTAAGCTAGGTTCACATAGAGAGTTCAGCTTTCTGGATGCGGAGAGTGATCCCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

502

Amino Acids

55.21

Weight (kDa)

5.5

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 23 - 65 6.1e-20 At5g58720 UBA-like domain
DUF1771 PF08590 340 - 403 1.7e-14 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 1109
AccB1I GGYRCC 1 cut(s) 946
AccB7I CCANNNNNTGG 1 cut(s) 912
AccI GTMKAC 1 cut(s) 682
AccII CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
AclI AACGTT 1 cut(s) 434
AclWI GGATC 2 cut(s) 259, 1490
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 3 cut(s) 237, 819, 1394
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 4 cut(s) 404, 786, 829, 1343
AfiI CCNNNNNNNGG 4 cut(s) 425, 645, 850, 912
AflIII ACRYGT 1 cut(s) 1252
AgsI TTSAA 5 cut(s) 590, 1098, 1319, 1391, 1415
AjnI CCWGG 3 cut(s) 419, 949, 1185
Alw21I GWGCWC 7 cut(s) 38, 236, 272, 413, 843, 1050, 1108
Alw26I GTCTC 1 cut(s) 552
AlwI GGATC 2 cut(s) 259, 1490
Ama87I CYCGRG 2 cut(s) 265, 271
AoxI GGCC 3 cut(s) 171, 366, 594
ApeKI GCWGC 3 cut(s) 479, 977, 1082
ApoI RAATTY 1 cut(s) 316
AseI ATTAAT 1 cut(s) 1445
Asp700I GAANNNNTTC 1 cut(s) 1410
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 428, 1065
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 693
AvaI CYCGRG 2 cut(s) 265, 271
AvaII GGWCC 2 cut(s) 428, 1065
AxyI CCTNAGG 1 cut(s) 1325
BanI GGYRCC 1 cut(s) 946
BanII GRGCYC 6 cut(s) 38, 236, 266, 272, 413, 843
BauI CACGAG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 7 cut(s) 38, 236, 272, 413, 843, 1050, 1108
BbvI GCAGC 3 cut(s) 466, 989, 1069
BccI CCATC 2 cut(s) 149, 1293
BceAI ACGGC 2 cut(s) 208, 1104
BciT130I CCWGG 3 cut(s) 421, 951, 1187
BciVI GTATCC 1 cut(s) 1137
BcoDI GTCTC 1 cut(s) 552
BfaI CTAG 3 cut(s) 972, 1203, 1454
BfmI CTRYAG 3 cut(s) 480, 640, 655
BfuAI ACCTGC 1 cut(s) 1109
BfuI GTATCC 1 cut(s) 1137
BisI GCNGC 6 cut(s) 174, 366, 369, 480, 978, 1083
BlpI GCTNAGC 2 cut(s) 498, 1131
BlsI GCNGC 6 cut(s) 175, 367, 370, 481, 979, 1084
BmcAI AGTACT 1 cut(s) 829
Bme1390I CCNGG 3 cut(s) 421, 951, 1187
Bme18I GGWCC 2 cut(s) 428, 1065
BmeT110I CYCGRG 2 cut(s) 265, 271
BmgT120I GGNCC 2 cut(s) 428, 1065
BmiI GGNNCC 2 cut(s) 429, 948
BmrFI CCNGG 3 cut(s) 421, 951, 1187
BmrI ACTGGG 1 cut(s) 1060
BmsI GCATC 5 cut(s) 118, 592, 694, 731, 1474
BmuI ACTGGG 1 cut(s) 1060
BoxI GACNNNNGTC 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 72
Bpu1102I GCTNAGC 2 cut(s) 498, 1131
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 632
BsaAI YACGTR 1 cut(s) 406
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 5 cut(s) 60, 174, 419, 597, 950
BsaWI WCCGGW 1 cut(s) 302
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 645, 850, 912
Bse1I ACTGG 1 cut(s) 1055
Bse21I CCTNAGG 1 cut(s) 1325
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 3 cut(s) 421, 951, 1187
BseDI CCNNGG 5 cut(s) 60, 174, 419, 597, 950
BseGI GGATG 2 cut(s) 607, 1489
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 4 cut(s) 425, 645, 850, 912
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 4 cut(s) 489, 530, 1122, 1339
BseNI ACTGG 1 cut(s) 1055
BseRI GAGGAG 2 cut(s) 1116, 1449
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 466, 989, 1069
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 173, 368, 596
BshNI GGYRCC 1 cut(s) 946
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 7 cut(s) 38, 236, 272, 413, 843, 1050, 1108
BsiHKCI CYCGRG 2 cut(s) 265, 271
BsiSI CCGG 3 cut(s) 303, 372, 833
BslFI GGGAC 2 cut(s) 870, 1312
BslI CCNNNNNNNGG 4 cut(s) 425, 645, 850, 912
BsmAI GTCTC 1 cut(s) 552
BsmBI CGTCTC 1 cut(s) 552
BsmFI GGGAC 2 cut(s) 870, 1312
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 173, 368, 596
BsoBI CYCGRG 2 cut(s) 265, 271
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 8 cut(s) 38, 236, 266, 272, 413, 843, 1050, 1108
Bsp143I GATC 3 cut(s) 251, 535, 1495
Bsp1720I GCTNAGC 2 cut(s) 498, 1131
Bsp68I TCGCGA 1 cut(s) 147
BspANI GGCC 3 cut(s) 173, 368, 596
BspCNI CTCAG 4 cut(s) 490, 531, 1123, 1338
BspFNI CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
BspLI GGNNCC 2 cut(s) 429, 948
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 1109
BspPI GGATC 2 cut(s) 259, 1490
BspQI GCTCTTC 2 cut(s) 26, 213
BspT104I TTCGAA 1 cut(s) 693
BspT107I GGYRCC 1 cut(s) 946
BsrDI GCAATG 1 cut(s) 605
BsrI ACTGG 1 cut(s) 1055
BssECI CCNNGG 5 cut(s) 60, 174, 419, 597, 950
BssMI GATC 3 cut(s) 251, 535, 1495
BssNI GRCGYC 1 cut(s) 561
BssSI CACGAG 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 60, 597
Bst2BI CACGAG 1 cut(s) 229
Bst2UI CCWGG 3 cut(s) 421, 951, 1187
Bst4CI ACNGT 5 cut(s) 379, 388, 636, 699, 1293
Bst6I CTCTTC 6 cut(s) 20, 26, 213, 347, 483, 1419
BstACI GRCGYC 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 262, 1183
BstDEI CTNAG 4 cut(s) 498, 539, 1131, 1325
BstDSI CCRYGG 1 cut(s) 174
BstEII GGTNACC 1 cut(s) 1121
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 2 cut(s) 607, 1489
BstFNI CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 3 cut(s) 254, 538, 1498
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 3 cut(s) 251, 535, 1495
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 6 cut(s) 137, 170, 602, 983, 1088, 1166
BstNI CCWGG 3 cut(s) 421, 951, 1187
BstNSI RCATGY 2 cut(s) 1118, 1256
BstPAI GACNNNNGTC 1 cut(s) 53
BstPI GGTNACC 1 cut(s) 1121
BstSCI CCNGG 3 cut(s) 419, 949, 1185
BstSFI CTRYAG 3 cut(s) 480, 640, 655
BstUI CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
BstV1I GCAGC 3 cut(s) 466, 989, 1069
BstV2I GAAGAC 1 cut(s) 72
BstZI CGGCCG 1 cut(s) 366
Bsu36I CCTNAGG 1 cut(s) 1325
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 3 cut(s) 173, 368, 596
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 607, 1489
BtsIMutI CAGTG 3 cut(s) 578, 1048, 1065
BtuMI TCGCGA 1 cut(s) 147
BveI ACCTGC 1 cut(s) 1109
Cac8I GCNNGC 2 cut(s) 262, 1183
CciNI GCGGCCGC 1 cut(s) 366
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 2 cut(s) 428, 1065
Cfr42I CCGCGG 1 cut(s) 177
CseI GACGC 3 cut(s) 58, 170, 266
Csp6I GTAC 4 cut(s) 403, 785, 828, 1342
CviAII CATG 7 cut(s) 907, 1022, 1115, 1244, 1253, 1267, 1352
CviQI GTAC 4 cut(s) 403, 785, 828, 1342
DdeI CTNAG 4 cut(s) 498, 539, 1131, 1325
DpnI GATC 3 cut(s) 253, 537, 1497
DpnII GATC 3 cut(s) 251, 535, 1495
DraI TTTAAA 2 cut(s) 1198, 1278
EaeI YGGCCR 1 cut(s) 366
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 6 cut(s) 20, 26, 213, 347, 483, 1419
EarI CTCTTC 6 cut(s) 20, 26, 213, 347, 483, 1419
Ecl136II GAGCTC 5 cut(s) 36, 234, 270, 411, 841
EclXI CGGCCG 1 cut(s) 366
Eco130I CCWWGG 2 cut(s) 60, 597
Eco147I AGGCCT 1 cut(s) 596
Eco24I GRGCYC 6 cut(s) 38, 236, 266, 272, 413, 843
Eco47I GGWCC 2 cut(s) 428, 1065
Eco52I CGGCCG 1 cut(s) 366
Eco53kI GAGCTC 5 cut(s) 36, 234, 270, 411, 841
Eco57I CTGAAG 3 cut(s) 237, 819, 1394
Eco81I CCTNAGG 1 cut(s) 1325
Eco88I CYCGRG 2 cut(s) 265, 271
Eco91I GGTNACC 1 cut(s) 1121
EcoICRI GAGCTC 5 cut(s) 36, 234, 270, 411, 841
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoO109I RGGNCCY 1 cut(s) 1065
EcoO65I GGTNACC 1 cut(s) 1121
EcoRII CCWGG 3 cut(s) 419, 949, 1185
EcoT14I CCWWGG 2 cut(s) 60, 597
EcoT22I ATGCAT 2 cut(s) 607, 1027
EcoT38I GRGCYC 6 cut(s) 38, 236, 266, 272, 413, 843
ErhI CCWWGG 2 cut(s) 60, 597
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 7 cut(s) 910, 1025, 1118, 1247, 1256, 1270, 1355
FaqI GGGAC 2 cut(s) 870, 1312
FatI CATG 7 cut(s) 906, 1021, 1114, 1243, 1252, 1266, 1351
FauI CCCGC 2 cut(s) 45, 253
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 6 cut(s) 174, 366, 369, 480, 978, 1083
FokI GGATG 2 cut(s) 614, 1496
FriOI GRGCYC 6 cut(s) 38, 236, 266, 272, 413, 843
Fsp4HI GCNGC 6 cut(s) 174, 366, 369, 480, 978, 1083
FspBI CTAG 3 cut(s) 972, 1203, 1454
GlaI GCGC 1 cut(s) 91
GluI GCNGC 6 cut(s) 174, 366, 369, 480, 978, 1083
HaeIII GGCC 3 cut(s) 173, 368, 596
HapII CCGG 3 cut(s) 303, 372, 833
HgaI GACGC 3 cut(s) 58, 170, 266
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 7 cut(s) 910, 1025, 1118, 1247, 1256, 1270, 1355
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 8 cut(s) 43, 299, 415, 530, 860, 883, 1055, 1429
HpaII CCGG 3 cut(s) 303, 372, 833
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 3 cut(s) 228, 683, 1460
Hpy188III TCNNGA 6 cut(s) 146, 229, 887, 1098, 1481, 1499
Hpy8I GTNNAC 3 cut(s) 228, 683, 1460
Hpy99I CGWCG 5 cut(s) 52, 115, 164, 289, 388
HpyAV CCTTC 7 cut(s) 73, 284, 325, 451, 700, 775, 1400
HpyCH4III ACNGT 5 cut(s) 379, 388, 636, 699, 1293
HpyCH4IV ACGT 4 cut(s) 405, 434, 561, 1301
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 170, 602, 983, 1088, 1166
HpyF3I CTNAG 4 cut(s) 498, 539, 1131, 1325
HpySE526I ACGT 4 cut(s) 405, 434, 561, 1301
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 7 cut(s) 910, 1025, 1118, 1247, 1256, 1270, 1355
HspAI GCGC 1 cut(s) 90
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 3 cut(s) 251, 535, 1495
LguI GCTCTTC 2 cut(s) 26, 213
LmnI GCTCC 5 cut(s) 846, 1045, 1103, 1419, 1436
Lsp1109I GCAGC 3 cut(s) 466, 989, 1069
LweI GCATC 5 cut(s) 118, 592, 694, 731, 1474
MaeI CTAG 3 cut(s) 972, 1203, 1454
MaeII ACGT 4 cut(s) 405, 434, 561, 1301
MaeIII GTNAC 2 cut(s) 1121, 1347
MalI GATC 3 cut(s) 253, 537, 1497
MboI GATC 3 cut(s) 251, 535, 1495
MfeI CAATTG 1 cut(s) 1233
MhlI GDGCHC 8 cut(s) 38, 236, 266, 272, 413, 843, 1050, 1108
MluCI AATT 8 cut(s) 316, 772, 910, 932, 1154, 1233, 1319, 1446
MlyI GAGTC 3 cut(s) 308, 539, 892
MmeI TCCRAC 4 cut(s) 71, 267, 455, 837
Mph1103I ATGCAT 2 cut(s) 607, 1027
MroXI GAANNNNTTC 1 cut(s) 1410
MseI TTAA 7 cut(s) 669, 870, 1005, 1197, 1277, 1445, 1449
MslI CAYNNNNRTG 1 cut(s) 1356
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 3 cut(s) 303, 372, 833
MspR9I CCNGG 3 cut(s) 421, 951, 1187
MunI CAATTG 1 cut(s) 1233
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 3 cut(s) 421, 951, 1187
MvnI CGCG 6 cut(s) 52, 90, 147, 164, 176, 260
MwoI GCNNNNNNNGC 6 cut(s) 137, 170, 602, 983, 1088, 1166
NdeII GATC 3 cut(s) 251, 535, 1495
NlaIII CATG 7 cut(s) 910, 1025, 1118, 1247, 1256, 1270, 1355
NlaIV GGNNCC 2 cut(s) 429, 948
NmeAIII GCCGAG 1 cut(s) 17
NmuCI GTSAC 1 cut(s) 1347
NotI GCGGCCGC 1 cut(s) 366
NruI TCGCGA 1 cut(s) 147
NsiI ATGCAT 2 cut(s) 607, 1027
NspI RCATGY 2 cut(s) 1118, 1256
NspV TTCGAA 1 cut(s) 693
PacI TTAATTAA 1 cut(s) 1449
PaeR7I CTCGAG 1 cut(s) 265
PceI AGGCCT 1 cut(s) 596
PciI ACATGT 1 cut(s) 1252
PciSI GCTCTTC 2 cut(s) 26, 213
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 1410
PfeI GAWTC 5 cut(s) 43, 415, 860, 1055, 1429
PflMI CCANNNNNTGG 1 cut(s) 912
PkrI GCNGC 6 cut(s) 175, 367, 370, 481, 979, 1084
PleI GAGTC 3 cut(s) 307, 538, 891
PpsI GAGTC 3 cut(s) 307, 538, 891
Ppu21I YACGTR 1 cut(s) 406
PpuMI RGGWCCY 1 cut(s) 1065
PscI ACATGT 1 cut(s) 1252
PshAI GACNNNNGTC 1 cut(s) 53
PshBI ATTAAT 1 cut(s) 1445
Psp124BI GAGCTC 5 cut(s) 38, 236, 272, 413, 843
Psp1406I AACGTT 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 1065
Psp6I CCWGG 3 cut(s) 419, 949, 1185
PspEI GGTNACC 1 cut(s) 1121
PspGI CCWGG 3 cut(s) 419, 949, 1185
PspN4I GGNNCC 2 cut(s) 429, 948
PspPI GGNCC 2 cut(s) 428, 1065
PspPPI RGGWCCY 1 cut(s) 1065
PspXI VCTCGAGB 1 cut(s) 265
PstI CTGCAG 1 cut(s) 484
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 4 cut(s) 404, 786, 829, 1343
RsaNI GTAC 4 cut(s) 403, 785, 828, 1342
RseI CAYNNNNRTG 1 cut(s) 1356
SacI GAGCTC 5 cut(s) 38, 236, 272, 413, 843
SacII CCGCGG 1 cut(s) 177
SapI GCTCTTC 2 cut(s) 26, 213
SaqAI TTAA 7 cut(s) 669, 870, 1005, 1197, 1277, 1445, 1449
SatI GCNGC 6 cut(s) 174, 366, 369, 480, 978, 1083
Sau3AI GATC 3 cut(s) 251, 535, 1495
Sau96I GGNCC 2 cut(s) 428, 1065
ScaI AGTACT 1 cut(s) 829
SchI GAGTC 3 cut(s) 308, 539, 892
ScrFI CCNGG 3 cut(s) 421, 951, 1187
SduI GDGCHC 8 cut(s) 38, 236, 266, 272, 413, 843, 1050, 1108
SfaNI GCATC 5 cut(s) 118, 592, 694, 731, 1474
SfcI CTRYAG 3 cut(s) 480, 640, 655
Sfr274I CTCGAG 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 693
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 2 cut(s) 428, 1065
SlaI CTCGAG 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 1356
SmlI CTYRAG 2 cut(s) 265, 611
SmoI CTYRAG 2 cut(s) 265, 611
Sse9I AATT 8 cut(s) 316, 772, 910, 932, 1154, 1233, 1319, 1446
SseBI AGGCCT 1 cut(s) 596
SspMI CTAG 3 cut(s) 972, 1203, 1454
SstI GAGCTC 5 cut(s) 38, 236, 272, 413, 843
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 3 cut(s) 419, 949, 1185
StyI CCWWGG 2 cut(s) 60, 597
TaaI ACNGT 5 cut(s) 379, 388, 636, 699, 1293
TaiI ACGT 4 cut(s) 408, 437, 564, 1304
TaqI TCGA 7 cut(s) 266, 297, 383, 413, 449, 693, 1432
TaqII GACCGA 1 cut(s) 1372
TasI AATT 8 cut(s) 316, 772, 910, 932, 1154, 1233, 1319, 1446
TatI WGTACW 1 cut(s) 827
TauI GCSGC 3 cut(s) 176, 368, 371
TfiI GAWTC 5 cut(s) 43, 415, 860, 1055, 1429
Tru1I TTAA 7 cut(s) 669, 870, 1005, 1197, 1277, 1445, 1449
Tru9I TTAA 7 cut(s) 669, 870, 1005, 1197, 1277, 1445, 1449
TscAI CASTG 3 cut(s) 578, 1055, 1065
TseFI GTSAC 1 cut(s) 1347
TseI GCWGC 3 cut(s) 479, 977, 1082
Tsp45I GTSAC 1 cut(s) 1347
TspDTI ATGAA 8 cut(s) 17, 645, 732, 909, 923, 1029, 1283, 1419
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 3 cut(s) 578, 1055, 1065
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 2 cut(s) 428, 1065
VspI ATTAAT 1 cut(s) 1445
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 1 cut(s) 316
XceI RCATGY 2 cut(s) 1118, 1256
XcmI CCANNNNNNNNNTGG 1 cut(s) 1292
XhoI CTCGAG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 1410
XspI CTAG 3 cut(s) 972, 1203, 1454
ZraI GACGTC 1 cut(s) 562
ZrmI AGTACT 1 cut(s) 829
Zsp2I ATGCAT 2 cut(s) 607, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.