Rh7AG204200

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001145
Physical Location & Seq
Reverse (-)
18612157 .. 18613492
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG204200.1

Sequence Viewer

Length: 480 bp
ATGATGTCCAAGACTCAGCGTTCTGTTGCTGCTATTACCGAAGAGGATGCTGAACAGTTTCTTTGCTCCATGTTTGGAGACGAGTCGGAGATCATAAGCTTGGGTGTGGTTAAAGATGTCCTTGGTCAGTGTGCGTATGAGGTTGAAAAGGCCTTGGATACCTTGCTCGAGTTATCTTCTTCCTCAAGTAAGAGACAGCGCTCTTCAGAATATACCCGCAAGAATTATGCCCAGGTTCTTGCTGGTCCTTCTCAAGCTCATAGGAGTACGAGTGCAAATCTGTCTCAAATGGTGTTGGAATCTTTGTTCAACAACTCAAAGAGAAGTCGTCAGAATAAAGAACCAAGAACTATGAGTTCTTGGAAGTATAGTGTCAAGGAGTTGCAAGATTCTTTGGGACCCGAGTTTGATGTTGCTGAGGGAGCTAAATATGATGCATACAGAAGTACTGCAAAGGATCGGAGCATTGGAATTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

159

Amino Acids

17.75

Weight (kDa)

5.93

Isoelectric Point (pI)

66.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 217
AclWI GGATC 1 cut(s) 465
AcsI RAATTY 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 189
AfaI GTAC 2 cut(s) 268, 448
AfeI AGCGCT 1 cut(s) 200
AgsI TTSAA 2 cut(s) 146, 310
AjnI CCWGG 1 cut(s) 231
AloI GAACNNNNNNTCC 2 cut(s) 290, 322
AluBI AGCT 3 cut(s) 99, 257, 425
AluI AGCT 3 cut(s) 99, 257, 425
Alw26I GTCTC 3 cut(s) 72, 187, 288
AlwI GGATC 1 cut(s) 465
Ama87I CYCGRG 2 cut(s) 167, 401
Aor51HI AGCGCT 1 cut(s) 200
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 471
Asp700I GAANNNNTTC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 245, 398
AvaI CYCGRG 2 cut(s) 167, 401
AvaII GGWCC 2 cut(s) 245, 398
BbvCI CCTCAGC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 16
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BciT130I CCWGG 1 cut(s) 233
BciVI GTATCC 1 cut(s) 151
BcoDI GTCTC 3 cut(s) 72, 187, 288
BfoI RGCGCY 1 cut(s) 202
BfuI GTATCC 1 cut(s) 151
BisI GCNGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 31
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 2 cut(s) 245, 398
BmeT110I CYCGRG 2 cut(s) 167, 401
BmgT120I GGNCC 2 cut(s) 245, 398
BmiI GGNNCC 2 cut(s) 399, 400
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 2 cut(s) 37, 424
Bpu10I CCTNAGC 1 cut(s) 417
BpuEI CTTGAG 2 cut(s) 169, 237
BsaJI CCNNGG 3 cut(s) 121, 153, 231
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
BseBI CCWGG 1 cut(s) 233
BseDI CCNNGG 3 cut(s) 121, 153, 231
BseGI GGATG 1 cut(s) 52
BseMII CTCAG 2 cut(s) 29, 408
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 152
BsiHKCI CYCGRG 2 cut(s) 167, 401
BslFI GGGAC 1 cut(s) 411
BsmAI GTCTC 3 cut(s) 72, 187, 288
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 1 cut(s) 411
BsnI GGCC 1 cut(s) 152
BsoBI CYCGRG 2 cut(s) 167, 401
Bsp143I GATC 2 cut(s) 90, 457
BspACI CCGC 1 cut(s) 217
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 2 cut(s) 28, 409
BspLI GGNNCC 2 cut(s) 399, 400
BspPI GGATC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 208
BssECI CCNNGG 3 cut(s) 121, 153, 231
BssMI GATC 2 cut(s) 90, 457
BssT1I CCWWGG 2 cut(s) 121, 153
Bst2UI CCWGG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 57
Bst6I CTCTTC 2 cut(s) 36, 208
BstDEI CTNAG 2 cut(s) 15, 417
BstF5I GGATG 1 cut(s) 52
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 2 cut(s) 93, 460
BstMAI GTCTC 3 cut(s) 72, 187, 288
BstMBI GATC 2 cut(s) 90, 457
BstMWI GCNNNNNNNGC 1 cut(s) 422
BstNI CCWGG 1 cut(s) 233
BstSCI CCNGG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 16
BsuI GTATCC 1 cut(s) 151
BsuRI GGCC 1 cut(s) 152
BtsCI GGATG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 134
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 245, 398
Csp6I GTAC 2 cut(s) 267, 447
CviAII CATG 1 cut(s) 70
CviJI RGCY 4 cut(s) 99, 152, 257, 425
CviKI_1 RGCY 4 cut(s) 99, 152, 257, 425
CviQI GTAC 2 cut(s) 267, 447
DdeI CTNAG 2 cut(s) 15, 417
DpnI GATC 2 cut(s) 92, 459
DpnII GATC 2 cut(s) 90, 457
Eam1104I CTCTTC 2 cut(s) 36, 208
EarI CTCTTC 2 cut(s) 36, 208
Eco130I CCWWGG 2 cut(s) 121, 153
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 2 cut(s) 245, 398
Eco47III AGCGCT 1 cut(s) 200
Eco57I CTGAAG 1 cut(s) 189
Eco88I CYCGRG 2 cut(s) 167, 401
EcoO109I RGGNCCY 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 471
EcoRII CCWGG 1 cut(s) 231
EcoT14I CCWWGG 2 cut(s) 121, 153
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 121, 153
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 1 cut(s) 73
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 1 cut(s) 411
FatI CATG 1 cut(s) 69
FauI CCCGC 1 cut(s) 224
Fnu4HI GCNGC 1 cut(s) 30
FokI GGATG 1 cut(s) 59
Fsp4HI GCNGC 1 cut(s) 30
GlaI GCGC 1 cut(s) 200
GluI GCNGC 1 cut(s) 30
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 1 cut(s) 152
HhaI GCGC 1 cut(s) 201
Hin1II CATG 1 cut(s) 73
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 4 cut(s) 13, 83, 299, 389
Hpy188I TCNGA 4 cut(s) 88, 208, 333, 462
HpyAV CCTTC 1 cut(s) 258
HpyCH4III ACNGT 1 cut(s) 57
HpyCH4V TGCA 4 cut(s) 275, 385, 437, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 422
HpyF3I CTNAG 2 cut(s) 15, 417
Hsp92II CATG 1 cut(s) 73
HspAI GCGC 1 cut(s) 199
KflI GGGWCCC 1 cut(s) 398
Kzo9I GATC 2 cut(s) 90, 457
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 3 cut(s) 71, 422, 462
LpnPI CCDG 3 cut(s) 218, 228, 245
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 2 cut(s) 37, 424
MalI GATC 2 cut(s) 92, 459
MboI GATC 2 cut(s) 90, 457
MboII GAAGA 4 cut(s) 53, 168, 171, 195
MluCI AATT 2 cut(s) 223, 471
MlyI GAGTC 2 cut(s) 7, 92
MmeI TCCRAC 2 cut(s) 66, 276
MnlI CCTC 4 cut(s) 37, 133, 193, 412
Mph1103I ATGCAT 1 cut(s) 439
MroXI GAANNNNTTC 1 cut(s) 57
MseI TTAA 1 cut(s) 111
MspR9I CCNGG 1 cut(s) 233
MvaI CCWGG 1 cut(s) 233
MwoI GCNNNNNNNGC 1 cut(s) 422
NdeII GATC 2 cut(s) 90, 457
NlaIII CATG 1 cut(s) 73
NlaIV GGNNCC 2 cut(s) 399, 400
NsiI ATGCAT 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 167
PceI AGGCCT 1 cut(s) 152
PciSI GCTCTTC 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 57
PfeI GAWTC 2 cut(s) 299, 389
PkrI GCNGC 1 cut(s) 31
PleI GAGTC 2 cut(s) 7, 91
PpsI GAGTC 2 cut(s) 7, 91
PpuMI RGGWCCY 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 231
PspGI CCWGG 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 399, 400
PspPI GGNCC 2 cut(s) 245, 398
PspPPI RGGWCCY 1 cut(s) 398
PspXI VCTCGAGB 1 cut(s) 167
RsaI GTAC 2 cut(s) 268, 448
RsaNI GTAC 2 cut(s) 267, 447
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 1 cut(s) 30
Sau3AI GATC 2 cut(s) 90, 457
Sau96I GGNCC 2 cut(s) 245, 398
ScaI AGTACT 1 cut(s) 448
SchI GAGTC 2 cut(s) 7, 92
ScrFI CCNGG 1 cut(s) 233
SetI ASST 6 cut(s) 101, 144, 164, 237, 259, 427
SfaNI GCATC 2 cut(s) 37, 424
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 2 cut(s) 245, 398
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 3 cut(s) 167, 184, 252
SmoI CTYRAG 3 cut(s) 167, 184, 252
Sse9I AATT 2 cut(s) 223, 471
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 1 cut(s) 217
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 231
StyI CCWWGG 2 cut(s) 121, 153
TaaI ACNGT 1 cut(s) 57
TaqI TCGA 1 cut(s) 168
TasI AATT 2 cut(s) 223, 471
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 2 cut(s) 299, 389
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 1 cut(s) 134
TseI GCWGC 1 cut(s) 29
TspRI CASTG 1 cut(s) 134
VpaK11BI GGWCC 2 cut(s) 245, 398
XapI RAATTY 1 cut(s) 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 239
XhoI CTCGAG 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 57
ZrmI AGTACT 1 cut(s) 448
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.