Rh7AG204500

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001145
Physical Location & Seq
Forward (+)
18666088 .. 18680146
14059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG204500.1

Sequence Viewer

Length: 1281 bp
ATGACGTCCAAGACTCGGCAGGGATCTCTTGCTGCTACAACCAAAGAGGATGCCGAGCAGTTTCTTTGCTCCATGTTTGGAGACGAGTCGGAGATAAGCTTGGGTGTGGTTAAAGATGTCCTTGGTCAGTGTGGGTATGAGGTTGAAAAGGCGTTGGATGCCTTGCTCGAGTTATCTTCTTCCTCAAGAAAGAGACGGTGCTCTTCAGAATATATCCGCAAGAACTATGCCGAGGTTCTTGTTGGTCCTTCTCAAGCACATAGGAGTACGAGTGCAAATCTGTCTCAGATGGTGGAATCTTTGTTTAACAACTCCAAGAGAAGTCTTCAGAATGAAGAACCAAGAACTATGAATTCTTGGAAGAACAGTGTCAAGGAATTGCAAGATTCTTTGGGACCCGAGTTTGATGTTGCTAAAGGAGCTGAATATGACGCATATAGAAGTACTGCAAAGGAGCATTGGGATTCAGTGAAGTCCTACCATCAAAAAGCTGCGACTGCGTATTCAAACGGATCACGGGCATATGCAGGTTACCTATCCGAGCAGGCCAAGGCAAAGGCGAAATTGGCACGAGAGGTGGACGAGAAGGCAAGCCAGGAGATCTTTAAAGCTAAAAACAAGGACAAGGGAATAAAAAATGATGTGATGACAATTGATTTGCATGGGCAGCATGTGAACGAAGCGATGAAGTTTTTGAAGATGCACATTCAGTATGGAACCAATACACAATCGCTTCGATTCCTCAGGGTTATTACAGGACATGGTGCAGGGAAGTCTATTGTGAAACACTCTGTCATCAAATTTTTCCAGAAAGAAGGTATTAAATGGAGTGAAGAGAATCCAGGAGCGTTGTTAATCAAGCTAGGTTCACAGAGAGACTACTTCAGATTTATGGACAGGGATGGTGAGGATGGATCTGCTCTTGATCCGTCAATCTATGGCGGCGGCCAAATTCCTCTTGTTGGCAGTGGTGGTGATCTGTGGCGGGACTGTATGCAACAGTATGGCGAATCTGGGCGAAGCAACGACTTCGTCTTCTTTTCCATGTCTTCGCCGTCTTCTGATGGTCCTAGCGGTGCAGATCTCGCGATCGGTGCAGTTGGTTGGCTTTGGGTGTTGACGGCAGGGTATATGGTTTTTGATGGTGGTGGATCTCAGGTTGTTGACATTGGAACTGTTCTGGTGGCCACCACTGGTGCTTGCGACGGCGATGGAGGCAGGATAGCAGCCAGCATCAATGTTGCATTTATCACGTCAGAGTTCAGACACCGTATCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

426

Amino Acids

46.64

Weight (kDa)

6.53

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 143 - 206 7e-15 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 518
AccII CGCG 1 cut(s) 1088
AciI CCGC 5 cut(s) 217, 942, 945, 985, 1074
AclWI GGATC 5 cut(s) 31, 520, 920, 922, 1159
AcoI YGGCCR 2 cut(s) 946, 1185
AcsI RAATTY 3 cut(s) 352, 800, 951
AcuI CTGAAG 3 cut(s) 189, 311, 868
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 268, 445
AfiI CCNNNNNNNGG 2 cut(s) 15, 962
AgsI TTSAA 3 cut(s) 146, 507, 697
AjiI CACGTC 1 cut(s) 1254
AjnI CCWGG 2 cut(s) 594, 841
AluBI AGCT 5 cut(s) 99, 422, 491, 611, 862
AluI AGCT 5 cut(s) 99, 422, 491, 611, 862
Alw21I GWGCWC 1 cut(s) 203
Alw26I GTCTC 4 cut(s) 75, 187, 288, 870
AlwI GGATC 5 cut(s) 31, 520, 920, 922, 1159
Ama87I CYCGRG 2 cut(s) 167, 398
AoxI GGCC 3 cut(s) 546, 946, 1185
ApeKI GCWGC 4 cut(s) 32, 491, 667, 1226
ApoI RAATTY 3 cut(s) 352, 800, 951
ArsI GACNNNNNNTTYG 2 cut(s) 640, 672
AspS9I GGNCC 3 cut(s) 245, 395, 1067
AsuHPI GGTGA 2 cut(s) 917, 986
AvaI CYCGRG 2 cut(s) 167, 398
AvaII GGWCC 3 cut(s) 245, 395, 1067
AxyI CCTNAGG 1 cut(s) 743
BalI TGGCCA 1 cut(s) 1187
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 4 cut(s) 317, 1027, 1041, 1050
Bbv12I GWGCWC 1 cut(s) 203
BbvI GCAGC 4 cut(s) 19, 478, 679, 1238
BccI CCATC 7 cut(s) 283, 489, 896, 905, 1058, 1136, 1205
BceAI ACGGC 3 cut(s) 1039, 1137, 1222
BcgI CGANNNNNNTGC 2 cut(s) 1012, 1046
BciT130I CCWGG 2 cut(s) 596, 843
BcoDI GTCTC 4 cut(s) 75, 187, 288, 870
BfaI CTAG 2 cut(s) 863, 1071
BfuAI ACCTGC 1 cut(s) 518
BglII AGATCT 2 cut(s) 600, 1081
BisI GCNGC 6 cut(s) 33, 492, 668, 943, 946, 1227
BlsI GCNGC 6 cut(s) 34, 493, 669, 944, 947, 1228
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 2 cut(s) 596, 843
Bme18I GGWCC 3 cut(s) 245, 395, 1067
BmeT110I CYCGRG 2 cut(s) 167, 398
BmgBI CACGTC 1 cut(s) 1254
BmgT120I GGNCC 3 cut(s) 245, 395, 1067
BmiI GGNNCC 3 cut(s) 396, 397, 718
BmrFI CCNGG 2 cut(s) 596, 843
BmsI GCATC 4 cut(s) 40, 148, 690, 1242
BpiI GAAGAC 4 cut(s) 317, 1027, 1041, 1050
BpuEI CTTGAG 2 cut(s) 169, 237
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 121, 231, 549
BsaXI ACNNNNNCTCC 2 cut(s) 256, 286
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 962
Bse1I ACTGG 1 cut(s) 1198
Bse21I CCTNAGG 1 cut(s) 743
BseBI CCWGG 2 cut(s) 596, 843
BseDI CCNNGG 3 cut(s) 121, 231, 549
BseGI GGATG 4 cut(s) 55, 163, 907, 916
BseLI CCNNNNNNNGG 2 cut(s) 15, 962
BseMII CTCAG 3 cut(s) 299, 757, 1169
BseNI ACTGG 1 cut(s) 1198
BseXI GCAGC 4 cut(s) 19, 478, 679, 1238
BsgI GTGCAG 3 cut(s) 786, 1098, 1116
Bsh1236I CGCG 1 cut(s) 1088
Bsh1285I CGRYCG 1 cut(s) 1092
BshFI GGCC 3 cut(s) 548, 948, 1187
BsiEI CGRYCG 1 cut(s) 1092
BsiHKAI GWGCWC 1 cut(s) 203
BsiHKCI CYCGRG 2 cut(s) 167, 398
BslFI GGGAC 2 cut(s) 408, 1001
BslI CCNNNNNNNGG 2 cut(s) 15, 962
BsmAI GTCTC 4 cut(s) 75, 187, 288, 870
BsmBI CGTCTC 2 cut(s) 75, 187
BsmFI GGGAC 2 cut(s) 408, 1001
BsnI GGCC 3 cut(s) 548, 948, 1187
BsoBI CYCGRG 2 cut(s) 167, 398
Bsp1286I GDGCHC 1 cut(s) 203
Bsp143I GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
Bsp68I TCGCGA 1 cut(s) 1088
BspACI CCGC 5 cut(s) 217, 942, 945, 985, 1074
BspANI GGCC 3 cut(s) 548, 948, 1187
BspCNI CTCAG 3 cut(s) 298, 756, 1168
BspFNI CGCG 1 cut(s) 1088
BspLI GGNNCC 3 cut(s) 396, 397, 718
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 5 cut(s) 31, 520, 920, 922, 1159
BspQI GCTCTTC 1 cut(s) 208
BsrI ACTGG 1 cut(s) 1198
BssECI CCNNGG 3 cut(s) 121, 231, 549
BssMI GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 2 cut(s) 121, 549
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 596, 843
Bst4CI ACNGT 6 cut(s) 198, 368, 992, 1002, 1177, 1271
Bst6I CTCTTC 2 cut(s) 208, 828
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 4 cut(s) 546, 592, 1201, 1231
BstDEI CTNAG 3 cut(s) 285, 743, 1155
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 4 cut(s) 55, 163, 907, 916
BstFNI CGCG 1 cut(s) 1088
BstKTI GATC 9 cut(s) 26, 515, 603, 917, 928, 979, 1084, 1092, 1154
BstMAI GTCTC 4 cut(s) 75, 187, 288, 870
BstMBI GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
BstMCI CGRYCG 1 cut(s) 1092
BstMWI GCNNNNNNNGC 8 cut(s) 158, 419, 497, 566, 667, 1085, 1094, 1215
BstNI CCWGG 2 cut(s) 596, 843
BstNSI RCATGY 1 cut(s) 674
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 2 cut(s) 594, 841
BstUI CGCG 1 cut(s) 1088
BstV1I GCAGC 4 cut(s) 19, 478, 679, 1238
BstV2I GAAGAC 4 cut(s) 317, 1027, 1041, 1050
BstX2I RGATCY 5 cut(s) 23, 600, 914, 1081, 1151
BstYI RGATCY 5 cut(s) 23, 600, 914, 1081, 1151
Bsu36I CCTNAGG 1 cut(s) 743
BsuRI GGCC 3 cut(s) 548, 948, 1187
BtgZI GCGATG 2 cut(s) 698, 1224
BtrI CACGTC 1 cut(s) 1254
BtsCI GGATG 4 cut(s) 55, 163, 907, 916
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 5 cut(s) 134, 373, 474, 973, 1191
BtuMI TCGCGA 1 cut(s) 1088
BveI ACCTGC 1 cut(s) 518
Cac8I GCNNGC 4 cut(s) 546, 592, 1201, 1231
Cfr13I GGNCC 3 cut(s) 245, 395, 1067
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 267, 444
CviAII CATG 5 cut(s) 73, 662, 671, 761, 1045
CviQI GTAC 2 cut(s) 267, 444
DdeI CTNAG 3 cut(s) 285, 743, 1155
DpnI GATC 9 cut(s) 25, 514, 602, 916, 927, 978, 1083, 1091, 1153
DpnII GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
DraI TTTAAA 1 cut(s) 607
EaeI YGGCCR 2 cut(s) 946, 1185
Eam1104I CTCTTC 2 cut(s) 208, 828
EarI CTCTTC 2 cut(s) 208, 828
Eco130I CCWWGG 2 cut(s) 121, 549
Eco47I GGWCC 3 cut(s) 245, 395, 1067
Eco57I CTGAAG 3 cut(s) 189, 311, 868
Eco81I CCTNAGG 1 cut(s) 743
Eco88I CYCGRG 2 cut(s) 167, 398
Eco91I GGTNACC 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 395
EcoO65I GGTNACC 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 594, 841
EcoT14I CCWWGG 2 cut(s) 121, 549
ErhI CCWWGG 2 cut(s) 121, 549
Esp3I CGTCTC 2 cut(s) 75, 187
FaeI CATG 5 cut(s) 76, 665, 674, 764, 1048
FalI AAGNNNNNCTT 2 cut(s) 105, 137
FaqI GGGAC 2 cut(s) 408, 1001
FatI CATG 5 cut(s) 72, 661, 670, 760, 1044
FauI CCCGC 1 cut(s) 978
FauNDI CATATG 1 cut(s) 523
Fnu4HI GCNGC 6 cut(s) 33, 492, 668, 943, 946, 1227
FokI GGATG 4 cut(s) 62, 170, 914, 923
Fsp4HI GCNGC 6 cut(s) 33, 492, 668, 943, 946, 1227
FspBI CTAG 2 cut(s) 863, 1071
GluI GCNGC 6 cut(s) 33, 492, 668, 943, 946, 1227
HaeIII GGCC 3 cut(s) 548, 948, 1187
HgaI GACGC 1 cut(s) 440
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 76, 665, 674, 764, 1048
HincII GTYRAC 2 cut(s) 1119, 1165
HindII GTYRAC 2 cut(s) 1119, 1165
HindIII AAGCTT 1 cut(s) 97
HinfI GANTC 8 cut(s) 13, 86, 296, 386, 464, 738, 838, 1010
HphI GGTGA 2 cut(s) 917, 986
Hpy166II GTNNAC 5 cut(s) 580, 676, 869, 1119, 1165
Hpy188I TCNGA 9 cut(s) 91, 208, 288, 330, 541, 887, 1063, 1258, 1265
Hpy188III TCNNGA 4 cut(s) 186, 808, 923, 1087
Hpy8I GTNNAC 5 cut(s) 580, 676, 869, 1119, 1165
Hpy99I CGWCG 1 cut(s) 1208
HpyAV CCTTC 3 cut(s) 258, 580, 809
HpyCH4III ACNGT 6 cut(s) 198, 368, 992, 1002, 1177, 1271
HpyCH4IV ACGT 2 cut(s) 5, 1253
HpyF10VI GCNNNNNNNGC 8 cut(s) 158, 419, 497, 566, 667, 1085, 1094, 1215
HpyF3I CTNAG 3 cut(s) 285, 743, 1155
HpySE526I ACGT 2 cut(s) 5, 1253
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 76, 665, 674, 764, 1048
KflI GGGWCCC 1 cut(s) 395
Kzo9I GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
LguI GCTCTTC 1 cut(s) 208
LmnI GCTCC 4 cut(s) 74, 419, 454, 845
Lsp1109I GCAGC 4 cut(s) 19, 478, 679, 1238
LweI GCATC 4 cut(s) 40, 148, 690, 1242
MaeI CTAG 2 cut(s) 863, 1071
MaeII ACGT 2 cut(s) 5, 1253
MaeIII GTNAC 1 cut(s) 530
MalI GATC 9 cut(s) 25, 514, 602, 916, 927, 978, 1083, 1091, 1153
MboI GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 5 cut(s) 23, 600, 914, 1081, 1151
MhlI GDGCHC 1 cut(s) 203
MlsI TGGCCA 1 cut(s) 1187
MluCI AATT 6 cut(s) 352, 377, 563, 651, 800, 951
MluNI TGGCCA 1 cut(s) 1187
MlyI GAGTC 2 cut(s) 7, 95
MmeI TCCRAC 2 cut(s) 69, 135
MnlI CCTC 9 cut(s) 40, 133, 193, 226, 568, 752, 901, 966, 1208
Mox20I TGGCCA 1 cut(s) 1187
MscI TGGCCA 1 cut(s) 1187
MseI TTAA 5 cut(s) 111, 306, 606, 822, 854
Msp20I TGGCCA 1 cut(s) 1187
MspR9I CCNGG 2 cut(s) 596, 843
MunI CAATTG 1 cut(s) 651
MvaI CCWGG 2 cut(s) 596, 843
MvnI CGCG 1 cut(s) 1088
MwoI GCNNNNNNNGC 8 cut(s) 158, 419, 497, 566, 667, 1085, 1094, 1215
NdeI CATATG 1 cut(s) 523
NdeII GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
NlaIII CATG 5 cut(s) 76, 665, 674, 764, 1048
NlaIV GGNNCC 3 cut(s) 396, 397, 718
NmeAIII GCCGAG 2 cut(s) 79, 256
NruI TCGCGA 1 cut(s) 1088
NspI RCATGY 1 cut(s) 674
PaeR7I CTCGAG 1 cut(s) 167
PciSI GCTCTTC 1 cut(s) 208
PfeI GAWTC 6 cut(s) 296, 386, 464, 738, 838, 1010
PflFI GACNNNGTC 1 cut(s) 1031
PfoI TCCNGGA 1 cut(s) 841
PkrI GCNGC 6 cut(s) 34, 493, 669, 944, 947, 1228
Ple19I CGATCG 1 cut(s) 1092
PleI GAGTC 2 cut(s) 7, 94
PpsI GAGTC 2 cut(s) 7, 94
PpuMI RGGWCCY 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 395
Psp6I CCWGG 2 cut(s) 594, 841
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 2 cut(s) 594, 841
PspN4I GGNNCC 3 cut(s) 396, 397, 718
PspPI GGNCC 3 cut(s) 245, 395, 1067
PspPPI RGGWCCY 1 cut(s) 395
PspXI VCTCGAGB 1 cut(s) 167
PsuI RGATCY 5 cut(s) 23, 600, 914, 1081, 1151
PsyI GACNNNGTC 1 cut(s) 1031
PvuI CGATCG 1 cut(s) 1092
RruI TCGCGA 1 cut(s) 1088
RsaI GTAC 2 cut(s) 268, 445
RsaNI GTAC 2 cut(s) 267, 444
SapI GCTCTTC 1 cut(s) 208
SaqAI TTAA 5 cut(s) 111, 306, 606, 822, 854
SatI GCNGC 6 cut(s) 33, 492, 668, 943, 946, 1227
Sau3AI GATC 9 cut(s) 23, 512, 600, 914, 925, 976, 1081, 1089, 1151
Sau96I GGNCC 3 cut(s) 245, 395, 1067
ScaI AGTACT 1 cut(s) 445
SchI GAGTC 2 cut(s) 7, 95
ScrFI CCNGG 2 cut(s) 596, 843
SduI GDGCHC 1 cut(s) 203
SfaNI GCATC 4 cut(s) 40, 148, 690, 1242
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 3 cut(s) 245, 395, 1067
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 3 cut(s) 167, 184, 252
SmoI CTYRAG 3 cut(s) 167, 184, 252
Sse9I AATT 6 cut(s) 352, 377, 563, 651, 800, 951
SsiI CCGC 5 cut(s) 217, 942, 945, 985, 1074
SspMI CTAG 2 cut(s) 863, 1071
StyD4I CCNGG 2 cut(s) 594, 841
StyI CCWWGG 2 cut(s) 121, 549
TaaI ACNGT 6 cut(s) 198, 368, 992, 1002, 1177, 1271
TaiI ACGT 2 cut(s) 8, 1256
TaqI TCGA 2 cut(s) 168, 736
TasI AATT 6 cut(s) 352, 377, 563, 651, 800, 951
TatI WGTACW 1 cut(s) 443
TauI GCSGC 2 cut(s) 945, 948
TfiI GAWTC 6 cut(s) 296, 386, 464, 738, 838, 1010
Tru1I TTAA 5 cut(s) 111, 306, 606, 822, 854
Tru9I TTAA 5 cut(s) 111, 306, 606, 822, 854
TscAI CASTG 5 cut(s) 134, 373, 474, 973, 1198
TseI GCWGC 4 cut(s) 32, 491, 667, 1226
TspDTI ATGAA 3 cut(s) 348, 365, 701
TspGWI ACGGA 2 cut(s) 525, 918
TspRI CASTG 5 cut(s) 134, 373, 474, 973, 1198
Tth111I GACNNNGTC 1 cut(s) 1031
VpaK11BI GGWCC 3 cut(s) 245, 395, 1067
XapI RAATTY 3 cut(s) 352, 800, 951
XceI RCATGY 1 cut(s) 674
XhoI CTCGAG 1 cut(s) 167
XspI CTAG 2 cut(s) 863, 1071
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.