FvH4_5g18840

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001145
Physical Location & Seq
Reverse (-)
10824788 .. 10826099
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18840.t1

Sequence Viewer

Length: 1041 bp
ATGAGCAACTGTTCAGGATATATTGCTGCAGACACTGCAAATCGTCGTGGGTTTCAAATGACATCTTGCAATGGTGCCCAGATTTTGATTCAGATTATGTCATCCATGAATCAGCGTTTTGGGAATGGATATGTTGCAGCAACTGCTACCAAAAAGGCTGCCGAGCAGTTTCTTCTTTCCATGTTTGGAGACGAGCCGGAGATAAGCTTGGGAGTGGTTCAAAAAGTCCTTGGTCGGTGTGGATACGAGGTCGAGAAGGCCTTGGATGCCTTGCTCGAGGTGTCTTCTTCATCAAGAAAGAGTCAGTGTTCTTCATCAGAACAAACCCGCAAGAACTATGCCGAGGTTCTTGCTGGTCCTCCTCCTCAAGCTCATAGCTGTATGGGTGCAAGTCTTTCTCACACGGTGATGGAGTCTTTGTTTAACAGCCCCAAGAGAAGTGATGAAGAACCAAGAACCATGGATTCTTGCAAGAAGCGTGTCGTCCATGATCATTCTTTAGGACCTGAGCTTGATGTTGCAAAGGGATCGGAATATGATGCGTATAGAAGAGAAGCGAAGCAGCACTGGGATTCAGTGAAGTCTTGCCATGAGAAAGCTGCGACCGCATATTCGAACGGATCGAAGGCATATGCAAGTTACTTGTCCGAGCACGCCAAGGCAAAGGTGAAAATGGCACGAGAAGCGGATGAGAAGGCAAGCCAGGAGATCTTTAAAGCTAAGAACAGGGACAAGGGCATAAAAGGTGATGTGTTGACAATCGATTTGCATGGGCAGCATGTTAACGAAGCCATGAAGCTGTTGAAGGTGCACATACAGTACGGTACCGACAGCCAGTCGCTTCGGTTCCTCAGGGTTATAACAGGACATGGTGCAGGGAAGTCTATTGTTAAACAATCTGTTATCAAGTTTTTCCAGAGAGAAGGTATTAAATACAGTGAAGAGAATCCAGGAGCGGTAATGATCAAGCTAGCTTCACAAAGAGACTGCTTCAGATATATGGACAGGGATGTACGGAGTACTATATGTTCAGTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

347

Amino Acids

38.23

Weight (kDa)

8.98

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 179 - 242 1.2e-15 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 860
Acc65I GGTACC 1 cut(s) 824
AccB1I GGYRCC 2 cut(s) 74, 824
AccBSI CCGCTC 1 cut(s) 956
AciI CCGC 4 cut(s) 328, 606, 686, 956
AclWI GGATC 2 cut(s) 535, 628
AcuI CTGAAG 1 cut(s) 976
AdeI CACNNNGTG 1 cut(s) 406
AfaI GTAC 4 cut(s) 821, 826, 1014, 1021
AgsI TTSAA 3 cut(s) 56, 221, 805
AhdI GACNNNNNGTC 1 cut(s) 835
AjnI CCWGG 2 cut(s) 702, 949
AluBI AGCT 9 cut(s) 207, 371, 378, 511, 599, 719, 799, 970, 974
AluI AGCT 9 cut(s) 207, 371, 378, 511, 599, 719, 799, 970, 974
Alw21I GWGCWC 2 cut(s) 654, 813
Alw26I GTCTC 2 cut(s) 183, 978
Alw44I GTGCAC 1 cut(s) 809
AlwI GGATC 2 cut(s) 535, 628
AlwNI CAGNNNCTG 2 cut(s) 35, 143
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 1 cut(s) 258
ApaLI GTGCAC 1 cut(s) 809
ApeKI GCWGC 6 cut(s) 26, 137, 158, 562, 599, 775
ArsI GACNNNNNNTTYG 4 cut(s) 595, 627, 748, 780
Asp718I GGTACC 1 cut(s) 824
AspS9I GGNCC 2 cut(s) 356, 503
AsuHPI GGTGA 3 cut(s) 418, 679, 758
AsuII TTCGAA 1 cut(s) 614
AsuNHI GCTAGC 1 cut(s) 970
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 2 cut(s) 356, 503
AxyI CCTNAGG 1 cut(s) 851
BaeGI GKGCMC 2 cut(s) 79, 813
BanI GGYRCC 2 cut(s) 74, 824
BauI CACGAG 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 276
Bbv12I GWGCWC 2 cut(s) 654, 813
BbvI GCAGC 6 cut(s) 13, 145, 149, 574, 586, 787
BccI CCATC 1 cut(s) 403
BcgI CGANNNNNNTGC 4 cut(s) 332, 366, 510, 544
BciT130I CCWGG 2 cut(s) 704, 951
BciVI GTATCC 1 cut(s) 236
BclI TGATCA 2 cut(s) 490, 963
BcoDI GTCTC 2 cut(s) 183, 978
BfaI CTAG 1 cut(s) 971
BfmI CTRYAG 1 cut(s) 27
BfuI GTATCC 1 cut(s) 236
BglII AGATCT 1 cut(s) 708
BisI GCNGC 6 cut(s) 27, 138, 159, 563, 600, 776
BlsI GCNGC 6 cut(s) 28, 139, 160, 564, 601, 777
BmcAI AGTACT 1 cut(s) 1021
Bme1390I CCNGG 2 cut(s) 704, 951
Bme18I GGWCC 2 cut(s) 356, 503
BmeRI GACNNNNNGTC 1 cut(s) 835
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 2 cut(s) 356, 503
BmiI GGNNCC 3 cut(s) 76, 826, 848
BmrFI CCNGG 2 cut(s) 704, 951
BmrI ACTGGG 1 cut(s) 577
BmsI GCATC 2 cut(s) 256, 529
BmtI GCTAGC 1 cut(s) 974
BmuI ACTGGG 1 cut(s) 577
BpiI GAAGAC 1 cut(s) 276
Bpu10I CCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 614
BpuEI CTTGAG 1 cut(s) 351
Bsa29I ATCGAT 1 cut(s) 762
BsaJI CCNNGG 5 cut(s) 229, 261, 342, 459, 657
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bse1I ACTGG 2 cut(s) 572, 835
Bse21I CCTNAGG 1 cut(s) 851
Bse3DI GCAATG 1 cut(s) 76
BseBI CCWGG 2 cut(s) 704, 951
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 5 cut(s) 229, 261, 342, 459, 657
BseGI GGATG 4 cut(s) 101, 271, 694, 1015
BseMI GCAATG 1 cut(s) 76
BseMII CTCAG 2 cut(s) 498, 865
BseNI ACTGG 2 cut(s) 572, 835
BseRI GAGGAG 2 cut(s) 351, 354
BseSI GKGCMC 2 cut(s) 79, 813
BseXI GCAGC 6 cut(s) 13, 145, 149, 574, 586, 787
BsgI GTGCAG 1 cut(s) 894
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 2 cut(s) 74, 824
BshVI ATCGAT 1 cut(s) 762
BsiEI CGRYCG 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 654, 813
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 197
BslFI GGGAC 1 cut(s) 743
BsmAI GTCTC 2 cut(s) 183, 978
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 743
BsnI GGCC 1 cut(s) 260
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 614
Bsp1286I GDGCHC 3 cut(s) 79, 654, 813
Bsp143I GATC 5 cut(s) 490, 527, 620, 708, 963
Bsp19I CCATGG 1 cut(s) 459
BspACI CCGC 4 cut(s) 328, 606, 686, 956
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 2 cut(s) 499, 864
BspDI ATCGAT 1 cut(s) 762
BspLI GGNNCC 3 cut(s) 76, 826, 848
BspMAI CTGCAG 1 cut(s) 31
BspOI GCTAGC 1 cut(s) 974
BspPI GGATC 2 cut(s) 535, 628
BspT104I TTCGAA 1 cut(s) 614
BspT107I GGYRCC 2 cut(s) 74, 824
BsrBI CCGCTC 1 cut(s) 956
BsrDI GCAATG 1 cut(s) 76
BsrI ACTGG 2 cut(s) 572, 835
BssECI CCNNGG 5 cut(s) 229, 261, 342, 459, 657
BssMI GATC 5 cut(s) 490, 527, 620, 708, 963
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 4 cut(s) 229, 261, 459, 657
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 2 cut(s) 704, 951
Bst4CI ACNGT 5 cut(s) 11, 406, 819, 824, 938
Bst6I CTCTTC 2 cut(s) 544, 936
BstAPI GCANNNNNTGC 2 cut(s) 35, 143
BstBI TTCGAA 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 654, 700, 972
BstDEI CTNAG 3 cut(s) 507, 720, 851
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 101, 271, 694, 1015
BstKTI GATC 5 cut(s) 493, 530, 623, 711, 966
BstMAI GTCTC 2 cut(s) 183, 978
BstMBI GATC 5 cut(s) 490, 527, 620, 708, 963
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 6 cut(s) 35, 143, 266, 605, 683, 775
BstNI CCWGG 2 cut(s) 704, 951
BstNSI RCATGY 1 cut(s) 782
BstSCI CCNGG 2 cut(s) 702, 949
BstSFI CTRYAG 1 cut(s) 27
BstSLI GKGCMC 2 cut(s) 79, 813
BstV1I GCAGC 6 cut(s) 13, 145, 149, 574, 586, 787
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 1 cut(s) 708
BstYI RGATCY 1 cut(s) 708
Bsu15I ATCGAT 1 cut(s) 762
Bsu36I CCTNAGG 1 cut(s) 851
BsuI GTATCC 1 cut(s) 236
BsuRI GGCC 1 cut(s) 260
BsuTUI ATCGAT 1 cut(s) 762
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 101, 271, 694, 1015
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 5 cut(s) 33, 311, 565, 582, 943
Cac8I GCNNGC 3 cut(s) 654, 700, 972
CaiI CAGNNNCTG 2 cut(s) 35, 143
Cfr13I GGNCC 2 cut(s) 356, 503
ClaI ATCGAT 1 cut(s) 762
Csp6I GTAC 4 cut(s) 820, 825, 1013, 1020
CviAII CATG 9 cut(s) 106, 181, 460, 488, 590, 770, 779, 793, 869
CviQI GTAC 4 cut(s) 820, 825, 1013, 1020
DdeI CTNAG 3 cut(s) 507, 720, 851
DpnI GATC 5 cut(s) 492, 529, 622, 710, 965
DpnII GATC 5 cut(s) 490, 527, 620, 708, 963
DraI TTTAAA 1 cut(s) 715
DraIII CACNNNGTG 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 835
Eam1104I CTCTTC 2 cut(s) 544, 936
Eam1105I GACNNNNNGTC 1 cut(s) 835
EarI CTCTTC 2 cut(s) 544, 936
Eco130I CCWWGG 4 cut(s) 229, 261, 459, 657
Eco147I AGGCCT 1 cut(s) 260
Eco47I GGWCC 2 cut(s) 356, 503
Eco57I CTGAAG 1 cut(s) 976
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 503
EcoRII CCWGG 2 cut(s) 702, 949
EcoT14I CCWWGG 4 cut(s) 229, 261, 459, 657
ErhI CCWWGG 4 cut(s) 229, 261, 459, 657
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 9 cut(s) 109, 184, 463, 491, 593, 773, 782, 796, 872
FaqI GGGAC 1 cut(s) 743
FatI CATG 9 cut(s) 105, 180, 459, 487, 589, 769, 778, 792, 868
FauI CCCGC 1 cut(s) 335
FauNDI CATATG 1 cut(s) 631
FbaI TGATCA 2 cut(s) 490, 963
Fnu4HI GCNGC 6 cut(s) 27, 138, 159, 563, 600, 776
FokI GGATG 4 cut(s) 88, 278, 701, 1022
Fsp4HI GCNGC 6 cut(s) 27, 138, 159, 563, 600, 776
FspBI CTAG 1 cut(s) 971
GluI GCNGC 6 cut(s) 27, 138, 159, 563, 600, 776
HaeIII GGCC 1 cut(s) 260
HapII CCGG 1 cut(s) 197
Hin1II CATG 9 cut(s) 109, 184, 463, 491, 593, 773, 782, 796, 872
HincII GTYRAC 2 cut(s) 756, 784
HindII GTYRAC 2 cut(s) 756, 784
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 7 cut(s) 88, 109, 301, 413, 464, 572, 946
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 1 cut(s) 197
HphI GGTGA 3 cut(s) 418, 679, 758
Hpy166II GTNNAC 3 cut(s) 756, 784, 811
Hpy188I TCNGA 5 cut(s) 93, 319, 532, 649, 995
Hpy188III TCNNGA 4 cut(s) 15, 253, 294, 916
Hpy8I GTNNAC 3 cut(s) 756, 784, 811
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 5 cut(s) 250, 619, 688, 799, 917
HpyCH4III ACNGT 5 cut(s) 11, 406, 819, 824, 938
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 143, 266, 605, 683, 775
HpyF3I CTNAG 3 cut(s) 507, 720, 851
Hsp92II CATG 9 cut(s) 109, 184, 463, 491, 593, 773, 782, 796, 872
KpnI GGTACC 1 cut(s) 828
Ksp22I TGATCA 2 cut(s) 490, 963
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 5 cut(s) 490, 527, 620, 708, 963
LmnI GCTCC 1 cut(s) 953
Lsp1109I GCAGC 6 cut(s) 13, 145, 149, 574, 586, 787
LweI GCATC 2 cut(s) 256, 529
MaeI CTAG 1 cut(s) 971
MaeIII GTNAC 1 cut(s) 638
MalI GATC 5 cut(s) 492, 529, 622, 710, 965
MbiI CCGCTC 1 cut(s) 956
MboI GATC 5 cut(s) 490, 527, 620, 708, 963
MboII GAAGA 7 cut(s) 164, 276, 279, 303, 458, 561, 953
MflI RGATCY 1 cut(s) 708
MhlI GDGCHC 3 cut(s) 79, 654, 813
MlyI GAGTC 2 cut(s) 310, 422
MnlI CCTC 7 cut(s) 241, 271, 337, 369, 372, 375, 860
MseI TTAA 5 cut(s) 423, 714, 783, 891, 930
MslI CAYNNNNRTG 1 cut(s) 407
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 2 cut(s) 704, 951
MvaI CCWGG 2 cut(s) 704, 951
MwoI GCNNNNNNNGC 6 cut(s) 35, 143, 266, 605, 683, 775
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 631
NdeII GATC 5 cut(s) 490, 527, 620, 708, 963
NheI GCTAGC 1 cut(s) 970
NlaIII CATG 9 cut(s) 109, 184, 463, 491, 593, 773, 782, 796, 872
NlaIV GGNNCC 3 cut(s) 76, 826, 848
NmeAIII GCCGAG 2 cut(s) 187, 367
NspI RCATGY 1 cut(s) 782
NspV TTCGAA 1 cut(s) 614
PaeR7I CTCGAG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 620
PfeI GAWTC 5 cut(s) 88, 109, 464, 572, 946
PfoI TCCNGGA 1 cut(s) 949
PkrI GCNGC 6 cut(s) 28, 139, 160, 564, 601, 777
PleI GAGTC 2 cut(s) 309, 421
PpsI GAGTC 2 cut(s) 309, 421
PpuMI RGGWCCY 1 cut(s) 503
PsiI TTATAA 1 cut(s) 860
Psp5II RGGWCCY 1 cut(s) 503
Psp6I CCWGG 2 cut(s) 702, 949
PspGI CCWGG 2 cut(s) 702, 949
PspN4I GGNNCC 3 cut(s) 76, 826, 848
PspPI GGNCC 2 cut(s) 356, 503
PspPPI RGGWCCY 1 cut(s) 503
PspXI VCTCGAGB 1 cut(s) 275
PsrI GAACNNNNNNTAC 1 cut(s) 1012
PstI CTGCAG 1 cut(s) 31
PstNI CAGNNNCTG 2 cut(s) 35, 143
PsuI RGATCY 1 cut(s) 708
RsaI GTAC 4 cut(s) 821, 826, 1014, 1021
RsaNI GTAC 4 cut(s) 820, 825, 1013, 1020
RseI CAYNNNNRTG 1 cut(s) 407
SaqAI TTAA 5 cut(s) 423, 714, 783, 891, 930
SatI GCNGC 6 cut(s) 27, 138, 159, 563, 600, 776
Sau3AI GATC 5 cut(s) 490, 527, 620, 708, 963
Sau96I GGNCC 2 cut(s) 356, 503
ScaI AGTACT 1 cut(s) 1021
SchI GAGTC 2 cut(s) 310, 422
ScrFI CCNGG 2 cut(s) 704, 951
SduI GDGCHC 3 cut(s) 79, 654, 813
SfaNI GCATC 2 cut(s) 256, 529
SfcI CTRYAG 1 cut(s) 27
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 614
SinI GGWCC 2 cut(s) 356, 503
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 407
SmlI CTYRAG 2 cut(s) 275, 366
SmoI CTYRAG 2 cut(s) 275, 366
SseBI AGGCCT 1 cut(s) 260
SsiI CCGC 4 cut(s) 328, 606, 686, 956
SspMI CTAG 1 cut(s) 971
StuI AGGCCT 1 cut(s) 260
StyD4I CCNGG 2 cut(s) 702, 949
StyI CCWWGG 4 cut(s) 229, 261, 459, 657
TaaI ACNGT 5 cut(s) 11, 406, 819, 824, 938
TaqI TCGA 5 cut(s) 252, 276, 614, 623, 762
TatI WGTACW 1 cut(s) 1019
TfiI GAWTC 5 cut(s) 88, 109, 464, 572, 946
Tru1I TTAA 5 cut(s) 423, 714, 783, 891, 930
Tru9I TTAA 5 cut(s) 423, 714, 783, 891, 930
TscAI CASTG 5 cut(s) 40, 311, 572, 582, 943
TseI GCWGC 6 cut(s) 26, 137, 158, 562, 599, 775
TspDTI ATGAA 5 cut(s) 122, 279, 303, 459, 809
TspGWI ACGGA 2 cut(s) 633, 1030
TspRI CASTG 5 cut(s) 40, 311, 572, 582, 943
VneI GTGCAC 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 356, 503
XceI RCATGY 1 cut(s) 782
XhoI CTCGAG 1 cut(s) 275
XspI CTAG 1 cut(s) 971
ZrmI AGTACT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.