Rorug05G0397800

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001145
Physical Location & Seq
Reverse (-)
54641169 .. 54644408
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0397800.1

Sequence Viewer

Length: 765 bp
ATGGCGACAGAGCAAGAGATATCGCAAGCTTTAGAAGCCCTGATCCACTCCTCCGACGCCCCGACGTTCGCCACTCTAAACGACGTCGTGCAGCAGCTCCAGTCCAAGCTCGGCGGCCACGACCTCTCGCACAAGGCCGACTTCATTCGGGCCCAGATCCACTACCTTCTCCAATCCCACCCGCCACCACCAACTCCCCAGCCTCAACCTCCTAAAGACCATTTTGCCCTCCAGCAAAACCCCAATTACCACCCGCCTCCCTCCGCCTTCCAGACCTTCGCCTCCCACCCTCCTCAACTTCCTTCTCCCAAGCCCAACCCCAACCCCAAGCCCAAGCCCGAGCCCAGCACTACTCCCGTACCCGCATCCGTTTCCGCCTCCGACCCGCCGCCTAAGGATGGCGCTAAACCTAAACCAAAGAGAAAAGGAGGCCCAGGGGGTCTAAACAAACTTTGTGGTGTTTCGCCTGAACTACAGGTCATTGTTGGCCATCCAACTCTTCCAAGGACTGAGATTGTGAAGCAACTGTGGGCTTACATAAGAAAACACAACCTCCAAGACCCCGGTAACAAGAGGAAAATTATTTGCAATGATGAGCTGCGGTTGGTTTTCGAGACAGACTGTACTGATATGTTTAAGATGAACAAGTTGCTGGCTAAACATATCATTGCACTTGAACCTTCAACCAGTTCCCAAGAAGCCCAAGGTTGTCGCGGACTCTGGAACAAAGAGTTCCCAGTCAGGTCCTTCTGTGATAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

254

Amino Acids

28.02

Weight (kDa)

8.92

Isoelectric Point (pI)

74.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEK_C PF08766 3 - 56 7.8e-10 DEK C terminal domain
SWIB PF02201 151 - 222 2.9e-27 SWIB/MDM2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AccII CGCG 1 cut(s) 714
AclWI GGATC 2 cut(s) 37, 151
AcoI YGGCCR 2 cut(s) 115, 487
AcyI GRCGYC 2 cut(s) 57, 84
AfaI GTAC 2 cut(s) 360, 625
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 2 cut(s) 677, 684
AjnI CCWGG 1 cut(s) 433
AluBI AGCT 4 cut(s) 29, 97, 109, 598
AluI AGCT 4 cut(s) 29, 97, 109, 598
Alw26I GTCTC 1 cut(s) 608
AlwI GGATC 2 cut(s) 37, 151
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 5 cut(s) 115, 135, 150, 430, 487
ApaI GGGCCC 1 cut(s) 154
ApeKI GCWGC 3 cut(s) 91, 94, 598
AspLEI GCGC 1 cut(s) 404
AspS9I GGNCC 4 cut(s) 150, 151, 431, 744
AsuC2I CCSGG 1 cut(s) 564
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 1 cut(s) 744
AxyI CCTNAGG 1 cut(s) 393
BaeGI GKGCMC 1 cut(s) 154
BaeI ACNNNNGTAYC 2 cut(s) 342, 375
BalI TGGCCA 1 cut(s) 489
BanII GRGCYC 2 cut(s) 154, 345
BbvI GCAGC 3 cut(s) 103, 106, 585
BccI CCATC 2 cut(s) 392, 498
BciT130I CCWGG 1 cut(s) 435
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 1 cut(s) 608
BfmI CTRYAG 1 cut(s) 473
BfoI RGCGCY 1 cut(s) 405
BisI GCNGC 5 cut(s) 92, 95, 115, 389, 599
BlsI GCNGC 5 cut(s) 93, 96, 116, 390, 600
Bme1390I CCNGG 2 cut(s) 435, 564
Bme18I GGWCC 1 cut(s) 744
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 4 cut(s) 150, 151, 431, 744
BmiI GGNNCC 1 cut(s) 152
BmrFI CCNGG 2 cut(s) 435, 564
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 1 cut(s) 374
BmuI ACTGGG 1 cut(s) 731
BpmI CTGGAG 2 cut(s) 83, 215
BpuMI CCSGG 1 cut(s) 564
BsaHI GRCGYC 2 cut(s) 57, 84
BsaJI CCNNGG 5 cut(s) 433, 434, 503, 562, 703
BsaXI ACNNNNNCTCC 2 cut(s) 537, 567
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 3 cut(s) 100, 687, 737
Bse21I CCTNAGG 1 cut(s) 393
Bse3DI GCAATG 2 cut(s) 595, 666
BseBI CCWGG 1 cut(s) 435
BseDI CCNNGG 5 cut(s) 433, 434, 503, 562, 703
BseGI GGATG 3 cut(s) 365, 403, 490
BseLI CCNNNNNNNGG 1 cut(s) 398
BseMI GCAATG 2 cut(s) 595, 666
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 3 cut(s) 100, 687, 737
BseRI GAGGAG 2 cut(s) 40, 282
BseSI GKGCMC 1 cut(s) 154
BseXI GCAGC 3 cut(s) 103, 106, 585
BseYI CCCAGC 2 cut(s) 198, 344
BsgI GTGCAG 1 cut(s) 110
Bsh1236I CGCG 1 cut(s) 714
BshFI GGCC 5 cut(s) 117, 137, 152, 432, 489
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 1 cut(s) 564
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 608
BsnI GGCC 5 cut(s) 117, 137, 152, 432, 489
BsoBI CYCGRG 1 cut(s) 338
Bsp120I GGGCCC 1 cut(s) 150
Bsp1286I GDGCHC 2 cut(s) 154, 345
Bsp143I GATC 2 cut(s) 42, 156
BspANI GGCC 5 cut(s) 117, 137, 152, 432, 489
BspCNI CTCAG 1 cut(s) 502
BspFNI CGCG 1 cut(s) 714
BspLI GGNNCC 1 cut(s) 152
BspPI GGATC 2 cut(s) 37, 151
BsrDI GCAATG 2 cut(s) 595, 666
BsrI ACTGG 3 cut(s) 100, 687, 737
BssECI CCNNGG 5 cut(s) 433, 434, 503, 562, 703
BssMI GATC 2 cut(s) 42, 156
BssNI GRCGYC 2 cut(s) 57, 84
BssT1I CCWWGG 2 cut(s) 503, 703
Bst2UI CCWGG 1 cut(s) 435
Bst4CI ACNGT 2 cut(s) 528, 623
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 2 cut(s) 57, 84
BstC8I GCNNGC 2 cut(s) 27, 654
BstDEI CTNAG 2 cut(s) 393, 510
BstF5I GGATG 3 cut(s) 365, 403, 490
BstFNI CGCG 1 cut(s) 714
BstH2I RGCGCY 1 cut(s) 405
BstHHI GCGC 1 cut(s) 404
BstKTI GATC 2 cut(s) 45, 159
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 2 cut(s) 42, 156
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 433, 562
BstSFI CTRYAG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 154
BstUI CGCG 1 cut(s) 714
BstV1I GCAGC 3 cut(s) 103, 106, 585
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
Bsu36I CCTNAGG 1 cut(s) 393
BsuRI GGCC 5 cut(s) 117, 137, 152, 432, 489
BtsCI GGATG 3 cut(s) 365, 403, 490
Cac8I GCNNGC 2 cut(s) 27, 654
CfoI GCGC 1 cut(s) 404
Cfr13I GGNCC 4 cut(s) 150, 151, 431, 744
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 359, 624
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviQI GTAC 2 cut(s) 359, 624
DdeI CTNAG 2 cut(s) 393, 510
DpnI GATC 2 cut(s) 44, 158
DpnII GATC 2 cut(s) 42, 156
EaeI YGGCCR 2 cut(s) 115, 487
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
EciI GGCGGA 2 cut(s) 253, 364
Eco130I CCWWGG 2 cut(s) 503, 703
Eco24I GRGCYC 2 cut(s) 154, 345
Eco32I GATATC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 744
Eco81I CCTNAGG 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 338
EcoO109I RGGNCCY 1 cut(s) 744
EcoRII CCWGG 1 cut(s) 433
EcoRV GATATC 1 cut(s) 21
EcoT14I CCWWGG 2 cut(s) 503, 703
EcoT38I GRGCYC 2 cut(s) 154, 345
ErhI CCWWGG 2 cut(s) 503, 703
FaiI YATR 3 cut(s) 539, 632, 663
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FauI CCCGC 4 cut(s) 189, 261, 370, 393
Fnu4HI GCNGC 5 cut(s) 92, 95, 115, 389, 599
FokI GGATG 3 cut(s) 352, 410, 477
FriOI GRGCYC 2 cut(s) 154, 345
Fsp4HI GCNGC 5 cut(s) 92, 95, 115, 389, 599
GlaI GCGC 1 cut(s) 403
GluI GCNGC 5 cut(s) 92, 95, 115, 389, 599
GsaI CCCAGC 2 cut(s) 202, 348
GsuI CTGGAG 2 cut(s) 83, 215
HaeII RGCGCY 1 cut(s) 405
HaeIII GGCC 5 cut(s) 117, 137, 152, 432, 489
HapII CCGG 1 cut(s) 564
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 404
Hin1I GRCGYC 2 cut(s) 57, 84
Hin6I GCGC 1 cut(s) 402
HinP1I GCGC 1 cut(s) 402
HindIII AAGCTT 1 cut(s) 27
HinfI GANTC 1 cut(s) 717
HpaII CCGG 1 cut(s) 564
Hpy188I TCNGA 3 cut(s) 55, 382, 764
Hpy188III TCNNGA 3 cut(s) 271, 613, 721
Hpy99I CGWCG 4 cut(s) 59, 67, 86, 89
HpyAV CCTTC 6 cut(s) 176, 277, 286, 312, 690, 757
HpyCH4III ACNGT 2 cut(s) 528, 623
HpyCH4IV ACGT 2 cut(s) 65, 84
HpyCH4V TGCA 3 cut(s) 91, 588, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpyF3I CTNAG 2 cut(s) 393, 510
HpySE526I ACGT 2 cut(s) 65, 84
Hsp92I GRCGYC 2 cut(s) 57, 84
HspAI GCGC 1 cut(s) 402
Kzo9I GATC 2 cut(s) 42, 156
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 103, 106, 585
LweI GCATC 1 cut(s) 374
MaeII ACGT 2 cut(s) 65, 84
MaeIII GTNAC 1 cut(s) 566
MalI GATC 2 cut(s) 44, 158
MboI GATC 2 cut(s) 42, 156
MboII GAAGA 1 cut(s) 491
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 154, 345
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 2 cut(s) 244, 579
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 711
MmeI TCCRAC 3 cut(s) 78, 405, 518
Mox20I TGGCCA 1 cut(s) 489
MscI TGGCCA 1 cut(s) 489
MseI TTAA 1 cut(s) 636
Msp20I TGGCCA 1 cut(s) 489
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 2 cut(s) 435, 564
MvaI CCWGG 1 cut(s) 435
MvnI CGCG 1 cut(s) 714
MwoI GCNNNNNNNGC 1 cut(s) 35
NciI CCSGG 1 cut(s) 564
NdeII GATC 2 cut(s) 42, 156
NlaIV GGNNCC 1 cut(s) 152
NmeAIII GCCGAG 1 cut(s) 90
PasI CCCWGGG 1 cut(s) 434
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PkrI GCNGC 5 cut(s) 93, 96, 116, 390, 600
PleI GAGTC 1 cut(s) 711
PpsI GAGTC 1 cut(s) 711
PpuMI RGGWCCY 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 744
Psp6I CCWGG 1 cut(s) 433
PspFI CCCAGC 2 cut(s) 198, 344
PspGI CCWGG 1 cut(s) 433
PspN4I GGNNCC 1 cut(s) 152
PspOMI GGGCCC 1 cut(s) 150
PspPI GGNCC 4 cut(s) 150, 151, 431, 744
PspPPI RGGWCCY 1 cut(s) 744
PsuI RGATCY 1 cut(s) 156
RsaI GTAC 2 cut(s) 360, 625
RsaNI GTAC 2 cut(s) 359, 624
SaqAI TTAA 1 cut(s) 636
SatI GCNGC 5 cut(s) 92, 95, 115, 389, 599
Sau3AI GATC 2 cut(s) 42, 156
Sau96I GGNCC 4 cut(s) 150, 151, 431, 744
SchI GAGTC 1 cut(s) 711
ScrFI CCNGG 2 cut(s) 435, 564
SduI GDGCHC 2 cut(s) 154, 345
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 473
SinI GGWCC 1 cut(s) 744
Sse9I AATT 2 cut(s) 244, 579
StyD4I CCNGG 2 cut(s) 433, 562
StyI CCWWGG 2 cut(s) 503, 703
TaaI ACNGT 2 cut(s) 528, 623
TaiI ACGT 2 cut(s) 68, 87
TaqI TCGA 1 cut(s) 612
TasI AATT 2 cut(s) 244, 579
TatI WGTACW 1 cut(s) 623
TauI GCSGC 2 cut(s) 117, 391
Tru1I TTAA 1 cut(s) 636
Tru9I TTAA 1 cut(s) 636
TseI GCWGC 3 cut(s) 91, 94, 598
TspDTI ATGAA 2 cut(s) 133, 656
TspGWI ACGGA 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 744
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.