RLG00000003238

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001145
Physical Location & Seq
Reverse (-)
45925004 .. 45928803
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003238

Sequence Viewer

Length: 1155 bp
ATGGGGTTCCTAAGGGTTGGTTTCGTTGCATCTTTCTTGGTGGGCTCTATGGTTCTTGTGCTGGATTCTGGGCGCCATGGTGATCCCACCATTGATGTTAGGTGGGGTCTAAGATGCAATGCAGTAGTGTCGTTTGATGGTGGTTATGGGTCGGGTCCCACAGCACGTGGTCTAGGCGTGTGGGTTGGGGTTTTGTCTAGTTGTGTTATGCTAGCTGCATGCCAAAGGAGAGGCTTTGTATCTAGTAATTTCATATCTGATTTTGGTATATCTGAGATGCTACAAGTGAGGAGACCAGCACGTCTACTTGGCCAAGCTCACCGAGCAGGCGGAGCGCTACGAGGAGATGGGTCCACCACGGCATCGGAGCTCACCGTCGAGGAGCGCATCGAGCAGAAGGAGGAGGGCCGCAAGAACAAGAAACTGTTCTTATCGTTGACGATGAAGAAGCAGACGAGGAGGAGAAGGAAGAAGAGCTCGCCGGCAGATCCGAAGCTGGCCGCTCCCCCGAAAGACGACGCCGAGCAGAAGGCAGTGGCCGAGCTGACGGAGGCCTTCTCTTCTGTCTCAGTCGACGACGCCATTTCGGCGCTTCTCGAAGCCAAAGGCCACTCGGACAAGGCCGCCGAGATTTTGGTCCAGTCTCTGGACGACACCTCCGACGAGCCGCTCACGAGCTTCACGTCGAGCGGCGTTTCTGGTTTTGATACCGGCTCCGACGAGCCGTTCACGAGCTCTATGTCGAGCGGCATTTCTGGTTTTGATACCGGCTCCGACGAGCCGTTCACGAGCTCTATGTCGAGCGGCATTTCTGGTTTTGATACCGGCTCTGACAATCCGTTCACGAGTTCCTTGTCCAGCAGTGTGTCCGGATCTGATACAAGCTCGACTTCTGGGTCGGGTTCGTCCGAGGGGTTTGAGTCATGTTGTGTTCAGAATTTAAAGAGGGTGGTTGCGTCGGCCGGAACGGTTTCGAATGTGTTGGGCAAAGAGAATGTGAGGTCGAGGATGACGAAGCTCAGTGGTTCAAAAAATGGAGATGCTGGTGGGGAAGAGGAAGCTGAACAGTTTCTTTGTTCAATGCTTGGAGATGACTCTAAGCTCAACTTGGCTGTTGTTAGAGATGTGCTTTGTAAGTGGCTATTGTTTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

385

Amino Acids

40.39

Weight (kDa)

5.26

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 173 - 214 9.3e-14 At5g58720 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 300, 895
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 1 cut(s) 646
AccBSI CCGCTC 5 cut(s) 503, 670, 690, 747, 804
AccI GTMKAC 2 cut(s) 304, 573
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 8 cut(s) 330, 409, 501, 624, 668, 690, 747, 804
AclWI GGATC 3 cut(s) 77, 482, 880
AcoI YGGCCR 4 cut(s) 310, 498, 537, 960
AcsI RAATTY 1 cut(s) 937
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 3 cut(s) 73, 519, 579
AfeI AGCGCT 1 cut(s) 336
AfiI CCNNNNNNNGG 1 cut(s) 646
AgsI TTSAA 2 cut(s) 1029, 1080
AjiI CACGTC 2 cut(s) 302, 684
Alw21I GWGCWC 4 cut(s) 372, 479, 737, 794
Alw26I GTCTC 3 cut(s) 286, 571, 648
AlwI GGATC 3 cut(s) 77, 482, 880
AlwNI CAGNNNCTG 1 cut(s) 646
Aor13HI TCCGGA 1 cut(s) 869
Aor51HI AGCGCT 1 cut(s) 336
AoxI GGCC 8 cut(s) 310, 406, 498, 537, 552, 607, 621, 960
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 1 cut(s) 937
Asp700I GAANNNNTTC 3 cut(s) 425, 970, 1068
AspLEI GCGC 4 cut(s) 75, 337, 387, 592
AspS9I GGNCC 4 cut(s) 155, 351, 406, 637
AsuHPI GGTGA 3 cut(s) 92, 311, 364
AsuII TTCGAA 1 cut(s) 974
AsuNHI GCTAGC 1 cut(s) 211
AvaII GGWCC 3 cut(s) 155, 351, 637
AxyI CCTNAGG 1 cut(s) 11
BalI TGGCCA 1 cut(s) 312
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 5 cut(s) 47, 372, 479, 737, 794
BauI CACGAG 4 cut(s) 673, 730, 787, 844
BbrPI CACGTG 1 cut(s) 167
Bbv12I GWGCWC 4 cut(s) 372, 479, 737, 794
BbvI GCAGC 1 cut(s) 202
BccI CCATC 2 cut(s) 131, 341
BceAI ACGGC 3 cut(s) 375, 709, 766
BcgI CGANNNNNNTGC 2 cut(s) 111, 145
BcoDI GTCTC 3 cut(s) 286, 571, 648
BfaI CTAG 4 cut(s) 173, 198, 212, 243
BfoI RGCGCY 3 cut(s) 76, 338, 593
BglI GCCNNNNNGGC 1 cut(s) 587
BisI GCNGC 8 cut(s) 216, 409, 501, 624, 668, 691, 748, 805
BlsI GCNGC 8 cut(s) 217, 410, 502, 625, 669, 692, 749, 806
Bme18I GGWCC 3 cut(s) 155, 351, 637
BmgBI CACGTC 2 cut(s) 302, 684
BmgT120I GGNCC 4 cut(s) 155, 351, 406, 637
BmiI GGNNCC 7 cut(s) 8, 74, 156, 157, 352, 715, 772
BmsI GCATC 6 cut(s) 38, 104, 267, 371, 396, 1030
BmtI GCTAGC 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 542, 574
Bpu14I TTCGAA 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 3 cut(s) 73, 519, 579
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 3 cut(s) 76, 357, 909
BsaWI WCCGGW 1 cut(s) 869
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 1 cut(s) 646
Bse118I RCCGGY 4 cut(s) 481, 710, 767, 824
Bse1I ACTGG 1 cut(s) 640
Bse21I CCTNAGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 124
BseAI TCCGGA 1 cut(s) 869
BseDI CCNNGG 3 cut(s) 76, 357, 909
BseGI GGATG 1 cut(s) 1014
BseLI CCNNNNNNNGG 1 cut(s) 646
BseMI GCAATG 1 cut(s) 124
BseMII CTCAG 3 cut(s) 264, 582, 1033
BseNI ACTGG 1 cut(s) 640
BseRI GAGGAG 6 cut(s) 304, 357, 395, 416, 472, 475
BseX3I CGGCCG 1 cut(s) 960
BseXI GCAGC 1 cut(s) 202
Bsh1285I CGRYCG 1 cut(s) 963
BshFI GGCC 8 cut(s) 312, 408, 500, 539, 554, 609, 623, 962
BshNI GGYRCC 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 963
BsiHKAI GWGCWC 4 cut(s) 372, 479, 737, 794
BsiSI CCGG 6 cut(s) 482, 711, 768, 825, 870, 963
BslFI GGGAC 1 cut(s) 141
BslI CCNNNNNNNGG 1 cut(s) 646
BsmAI GTCTC 3 cut(s) 286, 571, 648
BsmFI GGGAC 1 cut(s) 141
BsnI GGCC 8 cut(s) 312, 408, 500, 539, 554, 609, 623, 962
Bso31I GGTCTC 1 cut(s) 286
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 5 cut(s) 47, 372, 479, 737, 794
Bsp13I TCCGGA 1 cut(s) 869
Bsp143I GATC 3 cut(s) 82, 487, 872
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 8 cut(s) 330, 409, 501, 624, 668, 690, 747, 804
BspANI GGCC 8 cut(s) 312, 408, 500, 539, 554, 609, 623, 962
BspCNI CTCAG 3 cut(s) 265, 581, 1032
BspEI TCCGGA 1 cut(s) 869
BspLI GGNNCC 7 cut(s) 8, 74, 156, 157, 352, 715, 772
BspOI GCTAGC 1 cut(s) 215
BspPI GGATC 3 cut(s) 77, 482, 880
BspQI GCTCTTC 1 cut(s) 467
BspT104I TTCGAA 1 cut(s) 974
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 286
BsrBI CCGCTC 5 cut(s) 503, 670, 690, 747, 804
BsrDI GCAATG 1 cut(s) 124
BsrFI RCCGGY 4 cut(s) 481, 710, 767, 824
BsrI ACTGG 1 cut(s) 640
BssAI RCCGGY 4 cut(s) 481, 710, 767, 824
BssECI CCNNGG 3 cut(s) 76, 357, 909
BssMI GATC 3 cut(s) 82, 487, 872
BssNI GRCGYC 3 cut(s) 73, 519, 579
BssSI CACGAG 4 cut(s) 673, 730, 787, 844
BssT1I CCWWGG 1 cut(s) 76
Bst2BI CACGAG 4 cut(s) 673, 730, 787, 844
Bst4CI ACNGT 4 cut(s) 376, 426, 970, 1068
Bst6I CTCTTC 3 cut(s) 467, 565, 1047
BstACI GRCGYC 3 cut(s) 73, 519, 579
BstBAI YACGTR 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 974
BstC8I GCNNGC 6 cut(s) 213, 220, 328, 479, 483, 498
BstDEI CTNAG 6 cut(s) 11, 110, 273, 568, 1019, 1098
BstDSI CCRYGG 2 cut(s) 76, 357
BstF5I GGATG 1 cut(s) 1014
BstH2I RGCGCY 3 cut(s) 76, 338, 593
BstHHI GCGC 4 cut(s) 75, 337, 387, 592
BstKTI GATC 3 cut(s) 85, 490, 875
BstMAI GTCTC 3 cut(s) 286, 571, 648
BstMBI GATC 3 cut(s) 82, 487, 872
BstMCI CGRYCG 1 cut(s) 963
BstMWI GCNNNNNNNGC 4 cut(s) 323, 332, 391, 587
BstNSI RCATGY 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 487, 872
BstYI RGATCY 2 cut(s) 487, 872
BstZI CGGCCG 1 cut(s) 960
Bsu36I CCTNAGG 1 cut(s) 11
BsuRI GGCC 8 cut(s) 312, 408, 500, 539, 554, 609, 623, 962
BtgI CCRYGG 2 cut(s) 76, 357
BtrI CACGTC 2 cut(s) 302, 684
BtsCI GGATG 1 cut(s) 1014
BtsI GCAGTG 2 cut(s) 540, 868
BtsIMutI CAGTG 3 cut(s) 540, 868, 1027
Cac8I GCNNGC 6 cut(s) 213, 220, 328, 479, 483, 498
CaiI CAGNNNCTG 1 cut(s) 646
CfoI GCGC 4 cut(s) 75, 337, 387, 592
Cfr10I RCCGGY 4 cut(s) 481, 710, 767, 824
Cfr13I GGNCC 4 cut(s) 155, 351, 406, 637
CseI GACGC 3 cut(s) 527, 587, 945
CviAII CATG 3 cut(s) 77, 219, 924
DdeI CTNAG 6 cut(s) 11, 110, 273, 568, 1019, 1098
DinI GGCGCC 1 cut(s) 74
DpnI GATC 3 cut(s) 84, 489, 874
DpnII GATC 3 cut(s) 82, 487, 872
DraI TTTAAA 1 cut(s) 942
DrdI GACNNNNNNGTC 2 cut(s) 300, 895
DseDI GACNNNNNNGTC 2 cut(s) 300, 895
EaeI YGGCCR 4 cut(s) 310, 498, 537, 960
EagI CGGCCG 1 cut(s) 960
Eam1104I CTCTTC 3 cut(s) 467, 565, 1047
EarI CTCTTC 3 cut(s) 467, 565, 1047
EciI GGCGGA 1 cut(s) 345
Ecl136II GAGCTC 4 cut(s) 370, 477, 735, 792
EclXI CGGCCG 1 cut(s) 960
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 554
Eco24I GRGCYC 5 cut(s) 47, 372, 479, 737, 794
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 155, 351, 637
Eco47III AGCGCT 1 cut(s) 336
Eco52I CGGCCG 1 cut(s) 960
Eco53kI GAGCTC 4 cut(s) 370, 477, 735, 792
Eco72I CACGTG 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 11
EcoICRI GAGCTC 4 cut(s) 370, 477, 735, 792
EcoO109I RGGNCCY 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 76
EcoT38I GRGCYC 5 cut(s) 47, 372, 479, 737, 794
EgeI GGCGCC 1 cut(s) 74
EheI GGCGCC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 3 cut(s) 80, 222, 927
FalI AAGNNNNNCTT 4 cut(s) 874, 906, 1091, 1123
FaqI GGGAC 1 cut(s) 141
FatI CATG 3 cut(s) 76, 218, 923
FblI GTMKAC 2 cut(s) 304, 573
Fnu4HI GCNGC 8 cut(s) 216, 409, 501, 624, 668, 691, 748, 805
FokI GGATG 1 cut(s) 1021
FriOI GRGCYC 5 cut(s) 47, 372, 479, 737, 794
Fsp4HI GCNGC 8 cut(s) 216, 409, 501, 624, 668, 691, 748, 805
FspBI CTAG 4 cut(s) 173, 198, 212, 243
GlaI GCGC 4 cut(s) 74, 336, 386, 591
GluI GCNGC 8 cut(s) 216, 409, 501, 624, 668, 691, 748, 805
HaeII RGCGCY 3 cut(s) 76, 338, 593
HaeIII GGCC 8 cut(s) 312, 408, 500, 539, 554, 609, 623, 962
HapII CCGG 6 cut(s) 482, 711, 768, 825, 870, 963
HgaI GACGC 3 cut(s) 527, 587, 945
HhaI GCGC 4 cut(s) 75, 337, 387, 592
Hin1I GRCGYC 3 cut(s) 73, 519, 579
Hin1II CATG 3 cut(s) 80, 222, 927
Hin6I GCGC 4 cut(s) 73, 335, 385, 590
HinP1I GCGC 4 cut(s) 73, 335, 385, 590
HincII GTYRAC 2 cut(s) 438, 574
HindII GTYRAC 2 cut(s) 438, 574
HinfI GANTC 3 cut(s) 65, 920, 1094
HpaII CCGG 6 cut(s) 482, 711, 768, 825, 870, 963
HphI GGTGA 3 cut(s) 92, 311, 364
Hpy166II GTNNAC 7 cut(s) 305, 354, 438, 574, 729, 786, 843
Hpy188III TCNNGA 7 cut(s) 596, 647, 673, 730, 787, 844, 870
Hpy8I GTNNAC 7 cut(s) 305, 354, 438, 574, 729, 786, 843
Hpy99I CGWCG 9 cut(s) 380, 521, 578, 581, 665, 688, 722, 779, 961
HpyAV CCTTC 4 cut(s) 391, 459, 523, 565
HpyCH4III ACNGT 4 cut(s) 376, 426, 970, 1068
HpyCH4IV ACGT 3 cut(s) 166, 301, 683
HpyCH4V TGCA 4 cut(s) 29, 117, 122, 218
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 332, 391, 587
HpyF3I CTNAG 6 cut(s) 11, 110, 273, 568, 1019, 1098
HpySE526I ACGT 3 cut(s) 166, 301, 683
Hsp92I GRCGYC 3 cut(s) 73, 519, 579
Hsp92II CATG 3 cut(s) 80, 222, 927
HspAI GCGC 4 cut(s) 73, 335, 385, 590
KasI GGCGCC 1 cut(s) 72
KflI GGGWCCC 1 cut(s) 155
Kpn2I TCCGGA 1 cut(s) 869
KroI GCCGGC 1 cut(s) 481
KroNI GCCGGC 1 cut(s) 483
Kzo9I GATC 3 cut(s) 82, 487, 872
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 6 cut(s) 332, 367, 382, 508, 719, 776
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 6 cut(s) 38, 104, 267, 371, 396, 1030
MaeI CTAG 4 cut(s) 173, 198, 212, 243
MaeII ACGT 3 cut(s) 166, 301, 683
MalI GATC 3 cut(s) 84, 489, 874
MbiI CCGCTC 5 cut(s) 503, 670, 690, 747, 804
MboI GATC 3 cut(s) 82, 487, 872
MboII GAAGA 5 cut(s) 457, 481, 484, 552, 1064
MflI RGATCY 2 cut(s) 487, 872
MhlI GDGCHC 5 cut(s) 47, 372, 479, 737, 794
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 2 cut(s) 247, 937
MluNI TGGCCA 1 cut(s) 312
Mly113I GGCGCC 1 cut(s) 73
MlyI GAGTC 2 cut(s) 929, 1088
MmeI TCCRAC 3 cut(s) 684, 741, 798
Mox20I TGGCCA 1 cut(s) 312
MroI TCCGGA 1 cut(s) 869
MroNI GCCGGC 1 cut(s) 481
MroXI GAANNNNTTC 3 cut(s) 425, 970, 1068
MscI TGGCCA 1 cut(s) 312
MseI TTAA 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 6 cut(s) 482, 711, 768, 825, 870, 963
MwoI GCNNNNNNNGC 4 cut(s) 323, 332, 391, 587
NaeI GCCGGC 1 cut(s) 483
NarI GGCGCC 1 cut(s) 73
NcoI CCATGG 1 cut(s) 76
NdeII GATC 3 cut(s) 82, 487, 872
NgoMIV GCCGGC 1 cut(s) 481
NheI GCTAGC 1 cut(s) 211
NlaIII CATG 3 cut(s) 80, 222, 927
NlaIV GGNNCC 7 cut(s) 8, 74, 156, 157, 352, 715, 772
NmeAIII GCCGAG 3 cut(s) 547, 565, 652
NspI RCATGY 1 cut(s) 222
NspV TTCGAA 1 cut(s) 974
PaeI GCATGC 1 cut(s) 222
PceI AGGCCT 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 467
PcsI WCGNNNNNNNCGW 3 cut(s) 657, 680, 1010
PdiI GCCGGC 1 cut(s) 483
PdmI GAANNNNTTC 3 cut(s) 425, 970, 1068
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 646
PkrI GCNGC 8 cut(s) 217, 410, 502, 625, 669, 692, 749, 806
PleI GAGTC 2 cut(s) 928, 1088
PluTI GGCGCC 1 cut(s) 76
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
PpsI GAGTC 2 cut(s) 928, 1088
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 1 cut(s) 155
Psp124BI GAGCTC 4 cut(s) 372, 479, 737, 794
Psp5II RGGWCCY 1 cut(s) 155
PspCI CACGTG 1 cut(s) 167
PspN4I GGNNCC 7 cut(s) 8, 74, 156, 157, 352, 715, 772
PspPI GGNCC 4 cut(s) 155, 351, 406, 637
PspPPI RGGWCCY 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 2 cut(s) 487, 872
SacI GAGCTC 4 cut(s) 372, 479, 737, 794
SalI GTCGAC 1 cut(s) 572
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 941
SatI GCNGC 8 cut(s) 216, 409, 501, 624, 668, 691, 748, 805
Sau3AI GATC 3 cut(s) 82, 487, 872
Sau96I GGNCC 4 cut(s) 155, 351, 406, 637
SchI GAGTC 2 cut(s) 929, 1088
SduI GDGCHC 5 cut(s) 47, 372, 479, 737, 794
SfaNI GCATC 6 cut(s) 38, 104, 267, 371, 396, 1030
SfoI GGCGCC 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 3 cut(s) 155, 351, 637
SphI GCATGC 1 cut(s) 222
Sse9I AATT 2 cut(s) 247, 937
SseBI AGGCCT 1 cut(s) 554
SsiI CCGC 8 cut(s) 330, 409, 501, 624, 668, 690, 747, 804
SspDI GGCGCC 1 cut(s) 72
SspMI CTAG 4 cut(s) 173, 198, 212, 243
SstI GAGCTC 4 cut(s) 372, 479, 737, 794
StuI AGGCCT 1 cut(s) 554
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 4 cut(s) 376, 426, 970, 1068
TaiI ACGT 3 cut(s) 169, 304, 686
TasI AATT 2 cut(s) 247, 937
TauI GCSGC 7 cut(s) 411, 503, 626, 670, 693, 750, 807
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 1 cut(s) 941
Tru9I TTAA 1 cut(s) 941
TscAI CASTG 3 cut(s) 540, 868, 1027
TseI GCWGC 1 cut(s) 215
TspDTI ATGAA 2 cut(s) 241, 458
TspGWI ACGGA 2 cut(s) 563, 828
TspRI CASTG 3 cut(s) 540, 868, 1027
Van91I CCANNNNNTGG 1 cut(s) 646
VpaK11BI GGWCC 3 cut(s) 155, 351, 637
XapI RAATTY 1 cut(s) 937
XceI RCATGY 1 cut(s) 222
XmiI GTMKAC 2 cut(s) 304, 573
XmnI GAANNNNTTC 3 cut(s) 425, 970, 1068
XspI CTAG 4 cut(s) 173, 198, 212, 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.