Rmu_sc0002658.1_g000035

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002658.1
OG0001145
Physical Location & Seq
Forward (+)
149122 .. 150009
888 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002658.1_g000035.1.cds

Sequence Viewer

Length: 711 bp
atgaagaagcagacgaggaggagaaggaagaagagctcgccggcagatccgaagccggccgctccccccaaagacgacgccgagcagaaggcagtggccgcgctgacggaggccttctcttcggtctcagtcgacgacgccgtttcggcgcttcgcgaagccaaaggccacccagacaaggccgccgagattttggtccagtctctggccgacgacacctctgacgagccgttcacgagcttcacgtcgagcggcgtttctggtttcgataccggctccgacgagccgttcacgagctccatgtcgagtggcgtttccggttttgataccggctccgactatccgttcacgagttcctcgtccagcggcgtgtccggatctgatacgggctcgacttccgggtcgggttcgtccgaggggtttgagtcgggttgtgttcagaatttagtgggagagaggggttttaggaataagcagaagagggtggttgcgtcggccggaacggtttcgaatgtgttgggcaaagagtatgtgaggtcgaggatgacgaagctcagtggttcaaaaaatggagttgctgctggggaagaggaagctgaacagtttctttgttcaatgcttggggatgactctgagctcaacttggctgttgttagagatgtgctttgtcagtgtgggtatgatgttgaaaaggtactaagagcattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

236

Amino Acids

24.62

Weight (kDa)

4.93

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 205
AccBSI CCGCTC 2 cut(s) 62, 252
AccI GTMKAC 1 cut(s) 132
AccII CGCG 2 cut(s) 101, 156
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 5 cut(s) 60, 99, 183, 252, 366
AclWI GGATC 2 cut(s) 41, 385
AcoI YGGCCR 4 cut(s) 57, 96, 207, 495
AcsI RAATTY 1 cut(s) 442
AcyI GRCGYC 2 cut(s) 78, 138
AfaI GTAC 1 cut(s) 696
AfiI CCNNNNNNNGG 2 cut(s) 178, 205
AgsI TTSAA 3 cut(s) 564, 615, 689
AjiI CACGTC 1 cut(s) 246
AluBI AGCT 6 cut(s) 36, 240, 297, 553, 596, 637
AluI AGCT 6 cut(s) 36, 240, 297, 553, 596, 637
Alw21I GWGCWC 3 cut(s) 38, 299, 639
Alw26I GTCTC 2 cut(s) 130, 207
AlwI GGATC 2 cut(s) 41, 385
AlwNI CAGNNNCTG 1 cut(s) 205
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 7 cut(s) 57, 96, 111, 166, 180, 207, 495
ApeKI GCWGC 1 cut(s) 578
ApoI RAATTY 1 cut(s) 442
Asp700I GAANNNNTTC 2 cut(s) 505, 603
AspLEI GCGC 2 cut(s) 103, 151
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 400
AsuII TTCGAA 1 cut(s) 509
AvaII GGWCC 1 cut(s) 196
BanII GRGCYC 4 cut(s) 38, 299, 392, 639
BauI CACGAG 3 cut(s) 235, 292, 349
Bbv12I GWGCWC 3 cut(s) 38, 299, 639
BbvI GCAGC 1 cut(s) 565
BceAI ACGGC 3 cut(s) 125, 214, 271
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 2 cut(s) 130, 207
BfoI RGCGCY 1 cut(s) 152
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 6 cut(s) 60, 99, 183, 253, 367, 579
BlsI GCNGC 6 cut(s) 61, 100, 184, 254, 368, 580
Bme1390I CCNGG 1 cut(s) 400
Bme18I GGWCC 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 246
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 2 cut(s) 277, 334
BmrFI CCNGG 1 cut(s) 400
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu14I TTCGAA 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 400
BsaHI GRCGYC 2 cut(s) 78, 138
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 414
BsaWI WCCGGW 2 cut(s) 317, 374
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 205
Bse118I RCCGGY 4 cut(s) 40, 55, 272, 329
Bse1I ACTGG 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 414
BseGI GGATG 2 cut(s) 549, 631
BseLI CCNNNNNNNGG 2 cut(s) 178, 205
BseMII CTCAG 3 cut(s) 141, 568, 624
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 2 cut(s) 31, 34
BseX3I CGGCCG 2 cut(s) 57, 495
BseXI GCAGC 1 cut(s) 565
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 2 cut(s) 101, 156
Bsh1285I CGRYCG 2 cut(s) 60, 498
BshFI GGCC 7 cut(s) 59, 98, 113, 168, 182, 209, 497
BsiEI CGRYCG 2 cut(s) 60, 498
BsiHKAI GWGCWC 3 cut(s) 38, 299, 639
BsiSI CCGG 8 cut(s) 41, 56, 273, 318, 330, 375, 399, 498
BslI CCNNNNNNNGG 2 cut(s) 178, 205
BsmAI GTCTC 2 cut(s) 130, 207
BsnI GGCC 7 cut(s) 59, 98, 113, 168, 182, 209, 497
Bso31I GGTCTC 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 4 cut(s) 38, 299, 392, 639
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 2 cut(s) 46, 377
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 5 cut(s) 60, 99, 183, 252, 366
BspANI GGCC 7 cut(s) 59, 98, 113, 168, 182, 209, 497
BspCNI CTCAG 3 cut(s) 140, 567, 625
BspEI TCCGGA 1 cut(s) 374
BspFNI CGCG 2 cut(s) 101, 156
BspLI GGNNCC 2 cut(s) 277, 334
BspPI GGATC 2 cut(s) 41, 385
BspQI GCTCTTC 1 cut(s) 26
BspT104I TTCGAA 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 130
BsrBI CCGCTC 2 cut(s) 62, 252
BsrFI RCCGGY 4 cut(s) 40, 55, 272, 329
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 4 cut(s) 40, 55, 272, 329
BssECI CCNNGG 1 cut(s) 414
BssMI GATC 2 cut(s) 46, 377
BssNI GRCGYC 2 cut(s) 78, 138
BssSI CACGAG 3 cut(s) 235, 292, 349
Bst2BI CACGAG 3 cut(s) 235, 292, 349
Bst4CI ACNGT 2 cut(s) 505, 603
Bst6I CTCTTC 4 cut(s) 26, 124, 473, 582
BstACI GRCGYC 2 cut(s) 78, 138
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 38, 42, 57
BstDEI CTNAG 4 cut(s) 127, 554, 633, 698
BstF5I GGATG 2 cut(s) 549, 631
BstFNI CGCG 2 cut(s) 101, 156
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 103, 151
BstKTI GATC 2 cut(s) 49, 380
BstMAI GTCTC 2 cut(s) 130, 207
BstMBI GATC 2 cut(s) 46, 377
BstMCI CGRYCG 2 cut(s) 60, 498
BstMWI GCNNNNNNNGC 2 cut(s) 98, 146
BstSCI CCNGG 1 cut(s) 398
BstUI CGCG 2 cut(s) 101, 156
BstV1I GCAGC 1 cut(s) 565
BstX2I RGATCY 2 cut(s) 46, 377
BstYI RGATCY 2 cut(s) 46, 377
BstZI CGGCCG 2 cut(s) 57, 495
BsuRI GGCC 7 cut(s) 59, 98, 113, 168, 182, 209, 497
BtrI CACGTC 1 cut(s) 246
BtsCI GGATG 2 cut(s) 549, 631
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 562, 677
BtuMI TCGCGA 1 cut(s) 156
Cac8I GCNNGC 3 cut(s) 38, 42, 57
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 2 cut(s) 103, 151
Cfr10I RCCGGY 4 cut(s) 40, 55, 272, 329
Cfr13I GGNCC 1 cut(s) 196
CseI GACGC 3 cut(s) 86, 146, 480
Csp6I GTAC 1 cut(s) 695
CviAII CATG 1 cut(s) 301
CviQI GTAC 1 cut(s) 695
DdeI CTNAG 4 cut(s) 127, 554, 633, 698
DpnI GATC 2 cut(s) 48, 379
DpnII GATC 2 cut(s) 46, 377
DrdI GACNNNNNNGTC 1 cut(s) 400
DseDI GACNNNNNNGTC 1 cut(s) 400
EaeI YGGCCR 4 cut(s) 57, 96, 207, 495
EagI CGGCCG 2 cut(s) 57, 495
Eam1104I CTCTTC 4 cut(s) 26, 124, 473, 582
EarI CTCTTC 4 cut(s) 26, 124, 473, 582
Ecl136II GAGCTC 3 cut(s) 36, 297, 637
EclXI CGGCCG 2 cut(s) 57, 495
Eco147I AGGCCT 1 cut(s) 113
Eco24I GRGCYC 4 cut(s) 38, 299, 392, 639
Eco31I GGTCTC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 196
Eco52I CGGCCG 2 cut(s) 57, 495
Eco53kI GAGCTC 3 cut(s) 36, 297, 637
EcoICRI GAGCTC 3 cut(s) 36, 297, 637
EcoT38I GRGCYC 4 cut(s) 38, 299, 392, 639
FaeI CATG 1 cut(s) 304
FaiI YATR 3 cut(s) 302, 531, 681
FatI CATG 1 cut(s) 300
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 6 cut(s) 60, 99, 183, 253, 367, 579
FokI GGATG 2 cut(s) 556, 638
FriOI GRGCYC 4 cut(s) 38, 299, 392, 639
Fsp4HI GCNGC 6 cut(s) 60, 99, 183, 253, 367, 579
GlaI GCGC 2 cut(s) 102, 150
GluI GCNGC 6 cut(s) 60, 99, 183, 253, 367, 579
GsaI CCCAGC 1 cut(s) 585
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 7 cut(s) 59, 98, 113, 168, 182, 209, 497
HapII CCGG 8 cut(s) 41, 56, 273, 318, 330, 375, 399, 498
HgaI GACGC 3 cut(s) 86, 146, 480
HhaI GCGC 2 cut(s) 103, 151
Hin1I GRCGYC 2 cut(s) 78, 138
Hin1II CATG 1 cut(s) 304
Hin6I GCGC 2 cut(s) 101, 149
HinP1I GCGC 2 cut(s) 101, 149
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 2 cut(s) 425, 629
HpaII CCGG 8 cut(s) 41, 56, 273, 318, 330, 375, 399, 498
Hpy166II GTNNAC 4 cut(s) 133, 234, 291, 348
Hpy188I TCNGA 8 cut(s) 51, 223, 280, 337, 382, 415, 441, 634
Hpy188III TCNNGA 5 cut(s) 155, 235, 292, 349, 375
Hpy8I GTNNAC 4 cut(s) 133, 234, 291, 348
Hpy99I CGWCG 7 cut(s) 80, 137, 140, 215, 250, 284, 496
HpyAV CCTTC 3 cut(s) 18, 82, 124
HpyCH4III ACNGT 2 cut(s) 505, 603
HpyCH4IV ACGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 146
HpyF3I CTNAG 4 cut(s) 127, 554, 633, 698
HpySE526I ACGT 1 cut(s) 245
Hsp92I GRCGYC 2 cut(s) 78, 138
Hsp92II CATG 1 cut(s) 304
HspAI GCGC 2 cut(s) 101, 149
Kpn2I TCCGGA 1 cut(s) 374
KroI GCCGGC 2 cut(s) 40, 55
KroNI GCCGGC 2 cut(s) 42, 57
Kzo9I GATC 2 cut(s) 46, 377
LguI GCTCTTC 1 cut(s) 26
LmnI GCTCC 4 cut(s) 67, 281, 302, 338
Lsp1109I GCAGC 1 cut(s) 565
MaeII ACGT 1 cut(s) 245
MalI GATC 2 cut(s) 48, 379
MbiI CCGCTC 2 cut(s) 62, 252
MboI GATC 2 cut(s) 46, 377
MboII GAAGA 6 cut(s) 16, 40, 43, 111, 490, 599
MflI RGATCY 2 cut(s) 46, 377
MhlI GDGCHC 4 cut(s) 38, 299, 392, 639
MluCI AATT 1 cut(s) 442
MlyI GAGTC 2 cut(s) 434, 623
MmeI TCCRAC 2 cut(s) 303, 360
MroI TCCGGA 1 cut(s) 374
MroNI GCCGGC 2 cut(s) 40, 55
MroXI GAANNNNTTC 2 cut(s) 505, 603
MspA1I CMGCKG 1 cut(s) 366
MspI CCGG 8 cut(s) 41, 56, 273, 318, 330, 375, 399, 498
MspR9I CCNGG 1 cut(s) 400
MvnI CGCG 2 cut(s) 101, 156
MwoI GCNNNNNNNGC 2 cut(s) 98, 146
NaeI GCCGGC 2 cut(s) 42, 57
NciI CCSGG 1 cut(s) 400
NdeII GATC 2 cut(s) 46, 377
NgoMIV GCCGGC 2 cut(s) 40, 55
NlaIII CATG 1 cut(s) 304
NlaIV GGNNCC 2 cut(s) 277, 334
NmeAIII GCCGAG 2 cut(s) 106, 211
NruI TCGCGA 1 cut(s) 156
NspV TTCGAA 1 cut(s) 509
PceI AGGCCT 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 26
PcsI WCGNNNNNNNCGW 4 cut(s) 138, 242, 356, 545
PdiI GCCGGC 2 cut(s) 42, 57
PdmI GAANNNNTTC 2 cut(s) 505, 603
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 6 cut(s) 61, 100, 184, 254, 368, 580
PleI GAGTC 2 cut(s) 433, 623
PpsI GAGTC 2 cut(s) 433, 623
Psp124BI GAGCTC 3 cut(s) 38, 299, 639
PspFI CCCAGC 1 cut(s) 581
PspN4I GGNNCC 2 cut(s) 277, 334
PspPI GGNCC 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 2 cut(s) 46, 377
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 1 cut(s) 696
RsaNI GTAC 1 cut(s) 695
SacI GAGCTC 3 cut(s) 38, 299, 639
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 26
SatI GCNGC 6 cut(s) 60, 99, 183, 253, 367, 579
Sau3AI GATC 2 cut(s) 46, 377
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 2 cut(s) 434, 623
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 4 cut(s) 38, 299, 392, 639
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 1 cut(s) 196
Sse9I AATT 1 cut(s) 442
SseBI AGGCCT 1 cut(s) 113
SsiI CCGC 5 cut(s) 60, 99, 183, 252, 366
SstI GAGCTC 3 cut(s) 38, 299, 639
StuI AGGCCT 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 505, 603
TaiI ACGT 1 cut(s) 248
TaqI TCGA 7 cut(s) 132, 248, 267, 305, 392, 509, 539
TaqII GACCGA 1 cut(s) 112
TasI AATT 1 cut(s) 442
TauI GCSGC 5 cut(s) 62, 101, 185, 255, 369
TscAI CASTG 3 cut(s) 99, 562, 677
TseI GCWGC 1 cut(s) 578
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 122, 333
TspRI CASTG 3 cut(s) 99, 562, 677
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 2 cut(s) 505, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.