pycom03g05680

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001145
Physical Location & Seq
Reverse (-)
4511802 .. 4515277
3476 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05680.1

Sequence Viewer

Length: 912 bp
ATGAAGCACACGAAGAGGATAAGGAAGAGGAAGAGCTCGGCGAATCCGACGCGGGTCGCCAAGGAAGACGAAGAACAGAAGGTAATCCGCGCGCTTACGGAGGTTTTCTCGTCGGTATCGCTTGACGATGCCATTTATGCGTATCGCGAAGCCGATGGCGACGCGAACAAGGCCGCGGAGATTTTGGAACTGGCGGTGGCTGGAACTGAAAGTTCTGAAGAGCCGTTCACGAGCTCTACAACGGGTGGTGGATCGGACGCGGGCTCGAGCTCGGGTTCGGGTTCGTCGGAAGGGTTTGAGTCGGGTTGTATTCAGAATTTGGTGAGCGAGAAGGGTTTTAGGGGTAAGCAGAAGAGGGTTGTGGCGGCCGCCGGAACGGTATCGACGGTTTTGAGTAAGGAGTACGTGAGCTCGATTCCCAGGAGGGGTCCAACGTCGTCAGAGATGTCGAAGCGGAAGGATTTTGGAAATGGAGGTGCTGCAGGGGAAGAGGAAGCTGAGCAGTTTCTTTGTTCTATGCTTGGAGATGAGTCTGATCTGAGCTTGGCGGTTGTTAGAGACGTCCTTTGTCAGTGTGAGTATGATGTTGAAAAGGCCTTGGATGCATTGCTTGAGGTTTCTTCTTCGTACGAACAGTCCTGTAGTAGGTCTGACTATAGTATGTTTTTTAAGGAAGATAGTAGACATCCCTTCGAACAGTCTGATGCTCTGACAGATAGGGCATCTGATTGCAGTTCTCATTCATCTGAATATGAGCAGGATAATATATGGAATTATGGGAAGGTACTCACTAATTCTGAAGCTCATTCTCCTGTTAGCCCCAAAAGTACCCCGGCAGATCGATCCCTCAAAAGGTGTTGGAATCTTTGTTTAACATCTCCAAGAGTACCGAATATGAACCAAAAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

304

Amino Acids

32.93

Weight (kDa)

5.06

Isoelectric Point (pI)

59.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 23 - 63 1.3e-19 At5g58720 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 564
AccI GTMKAC 1 cut(s) 682
AccII CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
AclWI GGATC 2 cut(s) 259, 837
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 316
AcuI CTGAAG 2 cut(s) 237, 819
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 5 cut(s) 404, 629, 786, 829, 888
AfiI CCNNNNNNNGG 3 cut(s) 425, 645, 852
AgsI TTSAA 1 cut(s) 590
AjnI CCWGG 1 cut(s) 419
AluBI AGCT 7 cut(s) 36, 234, 270, 411, 497, 543, 803
AluI AGCT 7 cut(s) 36, 234, 270, 411, 497, 543, 803
Alw21I GWGCWC 4 cut(s) 38, 236, 272, 413
Alw26I GTCTC 1 cut(s) 552
AlwI GGATC 2 cut(s) 259, 837
Ama87I CYCGRG 2 cut(s) 265, 271
AoxI GGCC 3 cut(s) 171, 366, 594
ApeKI GCWGC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 316
AspLEI GCGC 2 cut(s) 92, 94
AspS9I GGNCC 1 cut(s) 428
AsuC2I CCSGG 1 cut(s) 833
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 693
AvaI CYCGRG 2 cut(s) 265, 271
AvaII GGWCC 1 cut(s) 428
BanII GRGCYC 5 cut(s) 38, 236, 266, 272, 413
BauI CACGAG 1 cut(s) 229
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 4 cut(s) 38, 236, 272, 413
BbvI GCAGC 1 cut(s) 466
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 1 cut(s) 421
BcnI CCSGG 1 cut(s) 833
BcoDI GTCTC 1 cut(s) 552
BfmI CTRYAG 3 cut(s) 480, 640, 655
BisI GCNGC 4 cut(s) 174, 366, 369, 480
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 4 cut(s) 175, 367, 370, 481
Bme1390I CCNGG 2 cut(s) 421, 833
Bme18I GGWCC 1 cut(s) 428
BmeT110I CYCGRG 2 cut(s) 265, 271
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 2 cut(s) 421, 833
BmsI GCATC 4 cut(s) 118, 592, 694, 731
BoxI GACNNNNGTC 1 cut(s) 53
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu1102I GCTNAGC 1 cut(s) 498
Bpu14I TTCGAA 1 cut(s) 693
BpuEI CTTGAG 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 833
Bsa29I ATCGAT 1 cut(s) 841
BsaAI YACGTR 1 cut(s) 406
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 5 cut(s) 60, 174, 419, 597, 831
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 645, 852
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 605
BseBI CCWGG 1 cut(s) 421
BseCI ATCGAT 1 cut(s) 841
BseDI CCNNGG 5 cut(s) 60, 174, 419, 597, 831
BseGI GGATG 2 cut(s) 607, 685
BseLI CCNNNNNNNGG 3 cut(s) 425, 645, 852
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 489, 530
BseNI ACTGG 1 cut(s) 195
BsePI GCGCGC 1 cut(s) 90
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 466
Bsh1236I CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 3 cut(s) 173, 368, 596
BshVI ATCGAT 1 cut(s) 841
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 4 cut(s) 38, 236, 272, 413
BsiHKCI CYCGRG 2 cut(s) 265, 271
BsiSI CCGG 2 cut(s) 372, 833
BsiWI CGTACG 1 cut(s) 627
BslI CCNNNNNNNGG 3 cut(s) 425, 645, 852
BsmAI GTCTC 1 cut(s) 552
BsmBI CGTCTC 1 cut(s) 552
BsnI GGCC 3 cut(s) 173, 368, 596
BsoBI CYCGRG 2 cut(s) 265, 271
Bsp119I TTCGAA 1 cut(s) 693
Bsp1286I GDGCHC 5 cut(s) 38, 236, 266, 272, 413
Bsp143I GATC 4 cut(s) 251, 535, 838, 842
Bsp1720I GCTNAGC 1 cut(s) 498
Bsp68I TCGCGA 1 cut(s) 147
BspANI GGCC 3 cut(s) 173, 368, 596
BspCNI CTCAG 2 cut(s) 490, 531
BspDI ATCGAT 1 cut(s) 841
BspFNI CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 484
BspPI GGATC 2 cut(s) 259, 837
BspQI GCTCTTC 2 cut(s) 26, 213
BspT104I TTCGAA 1 cut(s) 693
BsrDI GCAATG 1 cut(s) 605
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 5 cut(s) 60, 174, 419, 597, 831
BssHII GCGCGC 1 cut(s) 90
BssMI GATC 4 cut(s) 251, 535, 838, 842
BssNI GRCGYC 1 cut(s) 561
BssSI CACGAG 1 cut(s) 229
BssT1I CCWWGG 2 cut(s) 60, 597
Bst2BI CACGAG 1 cut(s) 229
Bst2UI CCWGG 1 cut(s) 421
Bst4CI ACNGT 4 cut(s) 379, 388, 636, 699
Bst6I CTCTTC 6 cut(s) 8, 20, 26, 213, 347, 483
BstACI GRCGYC 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 406
BstBI TTCGAA 1 cut(s) 693
BstC8I GCNNGC 2 cut(s) 92, 262
BstDEI CTNAG 2 cut(s) 498, 539
BstDSI CCRYGG 1 cut(s) 174
BstENI CCTNNNNNAGG 1 cut(s) 643
BstF5I GGATG 2 cut(s) 607, 685
BstFNI CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
BstHHI GCGC 2 cut(s) 92, 94
BstKTI GATC 4 cut(s) 254, 538, 841, 845
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 4 cut(s) 251, 535, 838, 842
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 3 cut(s) 137, 170, 602
BstNI CCWGG 1 cut(s) 421
BstPAI GACNNNNGTC 1 cut(s) 53
BstSCI CCNGG 2 cut(s) 419, 831
BstSFI CTRYAG 3 cut(s) 480, 640, 655
BstUI CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
BstV1I GCAGC 1 cut(s) 466
BstV2I GAAGAC 1 cut(s) 72
BstZI CGGCCG 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 841
BsuRI GGCC 3 cut(s) 173, 368, 596
BsuTUI ATCGAT 1 cut(s) 841
BtgI CCRYGG 1 cut(s) 174
BtsCI GGATG 2 cut(s) 607, 685
BtsIMutI CAGTG 1 cut(s) 578
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 2 cut(s) 92, 262
CciNI GCGGCCGC 1 cut(s) 366
CfoI GCGC 2 cut(s) 92, 94
Cfr13I GGNCC 1 cut(s) 428
Cfr42I CCGCGG 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 841
CseI GACGC 3 cut(s) 58, 170, 266
Csp6I GTAC 5 cut(s) 403, 628, 785, 828, 887
CviAII CATG 1 cut(s) 909
CviQI GTAC 5 cut(s) 403, 628, 785, 828, 887
DdeI CTNAG 2 cut(s) 498, 539
DpnI GATC 4 cut(s) 253, 537, 840, 844
DpnII GATC 4 cut(s) 251, 535, 838, 842
EaeI YGGCCR 1 cut(s) 366
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 6 cut(s) 8, 20, 26, 213, 347, 483
EarI CTCTTC 6 cut(s) 8, 20, 26, 213, 347, 483
Ecl136II GAGCTC 4 cut(s) 36, 234, 270, 411
EclXI CGGCCG 1 cut(s) 366
Eco130I CCWWGG 2 cut(s) 60, 597
Eco147I AGGCCT 1 cut(s) 596
Eco24I GRGCYC 5 cut(s) 38, 236, 266, 272, 413
Eco47I GGWCC 1 cut(s) 428
Eco52I CGGCCG 1 cut(s) 366
Eco53kI GAGCTC 4 cut(s) 36, 234, 270, 411
Eco57I CTGAAG 2 cut(s) 237, 819
Eco88I CYCGRG 2 cut(s) 265, 271
EcoICRI GAGCTC 4 cut(s) 36, 234, 270, 411
EcoNI CCTNNNNNAGG 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 419
EcoT14I CCWWGG 2 cut(s) 60, 597
EcoT22I ATGCAT 1 cut(s) 607
EcoT38I GRGCYC 5 cut(s) 38, 236, 266, 272, 413
ErhI CCWWGG 2 cut(s) 60, 597
Esp3I CGTCTC 1 cut(s) 552
FaeI CATG 1 cut(s) 912
FatI CATG 1 cut(s) 908
FauI CCCGC 2 cut(s) 45, 253
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 4 cut(s) 174, 366, 369, 480
FokI GGATG 2 cut(s) 614, 672
FriOI GRGCYC 5 cut(s) 38, 236, 266, 272, 413
Fsp4HI GCNGC 4 cut(s) 174, 366, 369, 480
GlaI GCGC 2 cut(s) 91, 93
GluI GCNGC 4 cut(s) 174, 366, 369, 480
HaeIII GGCC 3 cut(s) 173, 368, 596
HapII CCGG 2 cut(s) 372, 833
HgaI GACGC 3 cut(s) 58, 170, 266
HhaI GCGC 2 cut(s) 92, 94
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 1 cut(s) 912
Hin6I GCGC 2 cut(s) 90, 92
HinP1I GCGC 2 cut(s) 90, 92
HinfI GANTC 5 cut(s) 43, 299, 415, 530, 862
HpaII CCGG 2 cut(s) 372, 833
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 2 cut(s) 228, 683
Hpy188III TCNNGA 2 cut(s) 146, 229
Hpy8I GTNNAC 2 cut(s) 228, 683
Hpy99I CGWCG 6 cut(s) 52, 115, 164, 289, 388, 439
HpyAV CCTTC 6 cut(s) 73, 284, 325, 451, 700, 775
HpyCH4III ACNGT 4 cut(s) 379, 388, 636, 699
HpyCH4IV ACGT 3 cut(s) 405, 434, 561
HpyCH4V TGCA 3 cut(s) 482, 605, 732
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 170, 602
HpyF3I CTNAG 2 cut(s) 498, 539
HpySE526I ACGT 3 cut(s) 405, 434, 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 1 cut(s) 912
HspAI GCGC 2 cut(s) 90, 92
KspI CCGCGG 1 cut(s) 177
Kzo9I GATC 4 cut(s) 251, 535, 838, 842
LguI GCTCTTC 2 cut(s) 26, 213
Lsp1109I GCAGC 1 cut(s) 466
LweI GCATC 4 cut(s) 118, 592, 694, 731
MaeII ACGT 3 cut(s) 405, 434, 561
MalI GATC 4 cut(s) 253, 537, 840, 844
MboI GATC 4 cut(s) 251, 535, 838, 842
MhlI GDGCHC 5 cut(s) 38, 236, 266, 272, 413
MluCI AATT 3 cut(s) 316, 772, 793
MlyI GAGTC 2 cut(s) 308, 539
MmeI TCCRAC 4 cut(s) 71, 267, 455, 839
MnlI CCTC 9 cut(s) 9, 21, 94, 348, 417, 467, 484, 607, 857
Mph1103I ATGCAT 1 cut(s) 607
MseI TTAA 2 cut(s) 669, 872
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 2 cut(s) 372, 833
MspR9I CCNGG 2 cut(s) 421, 833
MvaI CCWGG 1 cut(s) 421
MvnI CGCG 7 cut(s) 52, 90, 92, 147, 164, 176, 260
MwoI GCNNNNNNNGC 3 cut(s) 137, 170, 602
NciI CCSGG 1 cut(s) 833
NdeII GATC 4 cut(s) 251, 535, 838, 842
NlaIII CATG 1 cut(s) 912
NlaIV GGNNCC 1 cut(s) 429
NmeAIII GCCGAG 1 cut(s) 17
NotI GCGGCCGC 1 cut(s) 366
NruI TCGCGA 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 607
NspV TTCGAA 1 cut(s) 693
PaeR7I CTCGAG 1 cut(s) 265
PauI GCGCGC 1 cut(s) 90
PceI AGGCCT 1 cut(s) 596
PciSI GCTCTTC 2 cut(s) 26, 213
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 3 cut(s) 43, 415, 862
Pfl23II CGTACG 1 cut(s) 627
PkrI GCNGC 4 cut(s) 175, 367, 370, 481
PleI GAGTC 2 cut(s) 307, 538
PpsI GAGTC 2 cut(s) 307, 538
Ppu21I YACGTR 1 cut(s) 406
PshAI GACNNNNGTC 1 cut(s) 53
Psp124BI GAGCTC 4 cut(s) 38, 236, 272, 413
Psp6I CCWGG 1 cut(s) 419
PspGI CCWGG 1 cut(s) 419
PspLI CGTACG 1 cut(s) 627
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 1 cut(s) 428
PspXI VCTCGAGB 1 cut(s) 265
PstI CTGCAG 1 cut(s) 484
PteI GCGCGC 1 cut(s) 90
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 5 cut(s) 404, 629, 786, 829, 888
RsaNI GTAC 5 cut(s) 403, 628, 785, 828, 887
SacI GAGCTC 4 cut(s) 38, 236, 272, 413
SacII CCGCGG 1 cut(s) 177
SapI GCTCTTC 2 cut(s) 26, 213
SaqAI TTAA 2 cut(s) 669, 872
SatI GCNGC 4 cut(s) 174, 366, 369, 480
Sau3AI GATC 4 cut(s) 251, 535, 838, 842
Sau96I GGNCC 1 cut(s) 428
SchI GAGTC 2 cut(s) 308, 539
ScrFI CCNGG 2 cut(s) 421, 833
SduI GDGCHC 5 cut(s) 38, 236, 266, 272, 413
SfaNI GCATC 4 cut(s) 118, 592, 694, 731
SfcI CTRYAG 3 cut(s) 480, 640, 655
Sfr274I CTCGAG 1 cut(s) 265
Sfr303I CCGCGG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 693
SgrBI CCGCGG 1 cut(s) 177
SinI GGWCC 1 cut(s) 428
SlaI CTCGAG 1 cut(s) 265
SmlI CTYRAG 2 cut(s) 265, 611
SmoI CTYRAG 2 cut(s) 265, 611
Sse9I AATT 3 cut(s) 316, 772, 793
SseBI AGGCCT 1 cut(s) 596
SstI GAGCTC 4 cut(s) 38, 236, 272, 413
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 2 cut(s) 419, 831
StyI CCWWGG 2 cut(s) 60, 597
TaaI ACNGT 4 cut(s) 379, 388, 636, 699
TaiI ACGT 3 cut(s) 408, 437, 564
TaqI TCGA 6 cut(s) 266, 383, 413, 449, 693, 841
TasI AATT 3 cut(s) 316, 772, 793
TauI GCSGC 3 cut(s) 176, 368, 371
TfiI GAWTC 3 cut(s) 43, 415, 862
Tru1I TTAA 2 cut(s) 669, 872
Tru9I TTAA 2 cut(s) 669, 872
TscAI CASTG 1 cut(s) 578
TseI GCWGC 1 cut(s) 479
TspDTI ATGAA 3 cut(s) 17, 732, 911
TspGWI ACGGA 1 cut(s) 113
TspRI CASTG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 428
XagI CCTNNNNNAGG 1 cut(s) 643
XapI RAATTY 1 cut(s) 316
XhoI CTCGAG 1 cut(s) 265
XmiI GTMKAC 1 cut(s) 682
ZraI GACGTC 1 cut(s) 562
Zsp2I ATGCAT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.