Rroxscaffold_3G00254090

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001145
Physical Location & Seq
Reverse (-)
48237510 .. 48238347
838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00254090.1

Sequence Viewer

Length: 813 bp
ATGCTCGGAGACGAGTCGGAGTTAAACTTGGGTGTTGTTAGGGATGTCCTTTCTCAGTGTGGGTATGAAGTTGAAAAGGCCTTGGATGCCCTACTTGAGTTATCTTCTTCCTCAAGTGAGACATGGTATTGTGTGGGAGATACTCACCAGAGTTATAAAGAGGTTCTTGCTGGTAGTACTTCCAAAGCTCACCGGAGTACGAGCTCAGATAACCTCTCTCAGGTGGTGTTGGAATCTTTGTTCAACAAATCTAAGAGGAGGAGTCCTGAAAATGAAGAACCAAGAACCATGAATTCTTGGAAGAATAGTGTGAAGGAATTGCAAGAGTCATTGGGACCTGAGCTTGTTGATGCCATAGGAGCTGAATACAATGCATATAGAGGGACTGCAAAGCAGCACTGGGATTCAGTAACGTCCCACTATCAGAAAGCTGCAACTGCGTATTCAAACGGATCGCGGGCCTATGCAGGTTACCTCTCTGAGCAGGCGAAGGCGGATGAGAAGGCAAGCCAGGAGATCTTCAAAGCTAGAAACAAGGATAAGTGGATAAACAATGGTGTGATAACTATTGATTTGCATGGCCAGCATGTCAAACAAGCAATGAAGCTTTTGAAAATCCACCTTCTGAAAGGAACCAATGCGCAGTCGCTTCGGCACCTCAGGGTTATCACCGGCTGTGGTGCAGGAAAGTCTATTCTGAAACAATCCGTCATCAGACTCATCCAGAACGAAGGTATTAAATGGAGCGAAGAGAATCCAGGAGCGGTATTAATCAAGCTAGCTTCACAGAGACAACTTCAGGTTCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

270

Amino Acids

29.91

Weight (kDa)

8.6

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1771 PF08590 123 - 178 1.6e-08 Domain of unknown function (DUF1771)
Smr PF01713 190 - 244 5e-06 Smr domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 156
Acc16I TGCGCA 1 cut(s) 642
Acc36I ACCTGC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 654
AccBSI CCGCTC 1 cut(s) 764
AccII CGCG 1 cut(s) 457
AciI CCGC 3 cut(s) 457, 494, 764
AclWI GGATC 1 cut(s) 460
AcoI YGGCCR 1 cut(s) 580
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 782
AfaI GTAC 2 cut(s) 178, 199
AfiI CCNNNNNNNGG 1 cut(s) 220
AgsI TTSAA 5 cut(s) 74, 244, 447, 523, 613
AjnI CCWGG 2 cut(s) 510, 757
AloI GAACNNNNNNTCC 2 cut(s) 224, 256
AluBI AGCT 9 cut(s) 188, 204, 343, 362, 431, 527, 607, 778, 782
AluI AGCT 9 cut(s) 188, 204, 343, 362, 431, 527, 607, 778, 782
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 3 cut(s) 3, 113, 784
AlwI GGATC 1 cut(s) 460
AoxI GGCC 3 cut(s) 78, 459, 580
ApeKI GCWGC 2 cut(s) 394, 431
ApoI RAATTY 1 cut(s) 292
AseI ATTAAT 1 cut(s) 770
AspLEI GCGC 1 cut(s) 643
AspS9I GGNCC 2 cut(s) 335, 459
AsuHPI GGTGA 3 cut(s) 137, 182, 661
AsuNHI GCTAGC 1 cut(s) 778
AvaII GGWCC 1 cut(s) 335
AxyI CCTNAGG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 582
BanI GGYRCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 206
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 2 cut(s) 406, 418
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciT130I CCWGG 2 cut(s) 512, 759
BcoDI GTCTC 3 cut(s) 3, 113, 784
BfaI CTAG 2 cut(s) 528, 779
BfuAI ACCTGC 1 cut(s) 458
BglII AGATCT 1 cut(s) 516
BisI GCNGC 2 cut(s) 395, 432
BlsI GCNGC 2 cut(s) 396, 433
BmcAI AGTACT 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 512, 759
Bme18I GGWCC 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 335, 459
BmiI GGNNCC 3 cut(s) 336, 634, 656
BmrFI CCNGG 2 cut(s) 512, 759
BmrI ACTGGG 1 cut(s) 409
BmsI GCATC 2 cut(s) 76, 340
BmtI GCTAGC 1 cut(s) 782
BmuI ACTGGG 1 cut(s) 409
Bpu10I CCTNAGC 1 cut(s) 339
BpuEI CTTGAG 2 cut(s) 97, 116
BsaJI CCNNGG 1 cut(s) 81
BsaWI WCCGGW 1 cut(s) 192
Bsc4I CCNNNNNNNGG 1 cut(s) 220
Bse118I RCCGGY 1 cut(s) 671
Bse1I ACTGG 1 cut(s) 404
Bse21I CCTNAGG 1 cut(s) 659
Bse3DI GCAATG 1 cut(s) 606
BseBI CCWGG 2 cut(s) 512, 759
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 4 cut(s) 49, 91, 502, 720
BseLI CCNNNNNNNGG 1 cut(s) 220
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 6 cut(s) 68, 219, 233, 330, 471, 673
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 2 cut(s) 271, 274
BseXI GCAGC 2 cut(s) 406, 418
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 457
BshFI GGCC 3 cut(s) 80, 461, 582
BshNI GGYRCC 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 206
BsiSI CCGG 2 cut(s) 193, 672
BslFI GGGAC 3 cut(s) 348, 397, 400
BslI CCNNNNNNNGG 1 cut(s) 220
BsmAI GTCTC 3 cut(s) 3, 113, 784
BsmBI CGTCTC 1 cut(s) 3
BsmFI GGGAC 3 cut(s) 348, 397, 400
BsnI GGCC 3 cut(s) 80, 461, 582
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 2 cut(s) 452, 516
BspACI CCGC 3 cut(s) 457, 494, 764
BspANI GGCC 3 cut(s) 80, 461, 582
BspCNI CTCAG 6 cut(s) 67, 218, 232, 331, 472, 672
BspFNI CGCG 1 cut(s) 457
BspLI GGNNCC 3 cut(s) 336, 634, 656
BspMI ACCTGC 1 cut(s) 458
BspOI GCTAGC 1 cut(s) 782
BspPI GGATC 1 cut(s) 460
BspT107I GGYRCC 1 cut(s) 654
BsrBI CCGCTC 1 cut(s) 764
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 671
BsrI ACTGG 1 cut(s) 404
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 2 cut(s) 452, 516
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 2 cut(s) 512, 759
Bst6I CTCTTC 1 cut(s) 744
BstC8I GCNNGC 5 cut(s) 459, 486, 508, 584, 780
BstDEI CTNAG 7 cut(s) 54, 205, 219, 252, 339, 480, 659
BstEII GGTNACC 1 cut(s) 470
BstENI CCTNNNNNAGG 1 cut(s) 218
BstF5I GGATG 4 cut(s) 49, 91, 502, 720
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 1 cut(s) 643
BstKTI GATC 2 cut(s) 455, 519
BstMAI GTCTC 3 cut(s) 3, 113, 784
BstMBI GATC 2 cut(s) 452, 516
BstMWI GCNNNNNNNGC 4 cut(s) 86, 359, 437, 583
BstNI CCWGG 2 cut(s) 512, 759
BstNSI RCATGY 1 cut(s) 590
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 2 cut(s) 510, 757
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 2 cut(s) 406, 418
BstX2I RGATCY 1 cut(s) 516
BstYI RGATCY 1 cut(s) 516
Bsu36I CCTNAGG 1 cut(s) 659
BsuRI GGCC 3 cut(s) 80, 461, 582
BtsCI GGATG 4 cut(s) 49, 91, 502, 720
BtsIMutI CAGTG 2 cut(s) 62, 397
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 5 cut(s) 459, 486, 508, 584, 780
CfoI GCGC 1 cut(s) 643
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 2 cut(s) 335, 459
Csp6I GTAC 2 cut(s) 177, 198
CviAII CATG 5 cut(s) 123, 289, 578, 587, 806
CviQI GTAC 2 cut(s) 177, 198
DdeI CTNAG 7 cut(s) 54, 205, 219, 252, 339, 480, 659
DpnI GATC 2 cut(s) 454, 518
DpnII GATC 2 cut(s) 452, 516
EaeI YGGCCR 1 cut(s) 580
Eam1104I CTCTTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 744
EciI GGCGGA 1 cut(s) 509
Ecl136II GAGCTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 81
Eco147I AGGCCT 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 335
Eco53kI GAGCTC 1 cut(s) 204
Eco57I CTGAAG 1 cut(s) 782
Eco81I CCTNAGG 1 cut(s) 659
Eco91I GGTNACC 1 cut(s) 470
EcoICRI GAGCTC 1 cut(s) 204
EcoNI CCTNNNNNAGG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 335
EcoO65I GGTNACC 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 292
EcoRII CCWGG 2 cut(s) 510, 757
EcoT14I CCWWGG 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 3
FaeI CATG 5 cut(s) 126, 292, 581, 590, 809
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 3 cut(s) 348, 397, 400
FatI CATG 5 cut(s) 122, 288, 577, 586, 805
FauI CCCGC 1 cut(s) 450
Fnu4HI GCNGC 2 cut(s) 395, 432
FokI GGATG 4 cut(s) 56, 98, 509, 707
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 395, 432
FspBI CTAG 2 cut(s) 528, 779
FspI TGCGCA 1 cut(s) 642
GlaI GCGC 1 cut(s) 642
GluI GCNGC 2 cut(s) 395, 432
HaeIII GGCC 3 cut(s) 80, 461, 582
HapII CCGG 2 cut(s) 193, 672
HhaI GCGC 1 cut(s) 643
Hin1II CATG 5 cut(s) 126, 292, 581, 590, 809
Hin6I GCGC 1 cut(s) 641
HinP1I GCGC 1 cut(s) 641
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 7 cut(s) 14, 233, 262, 326, 404, 717, 754
HpaII CCGG 2 cut(s) 193, 672
HphI GGTGA 3 cut(s) 137, 182, 661
Hpy188I TCNGA 8 cut(s) 8, 19, 208, 426, 481, 627, 699, 716
Hpy188III TCNNGA 2 cut(s) 266, 724
HpyAV CCTTC 5 cut(s) 307, 484, 496, 632, 725
HpyCH4IV ACGT 1 cut(s) 413
HpyCH4V TGCA 7 cut(s) 322, 374, 389, 434, 467, 577, 683
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 359, 437, 583
HpyF3I CTNAG 7 cut(s) 54, 205, 219, 252, 339, 480, 659
HpySE526I ACGT 1 cut(s) 413
Hsp92II CATG 5 cut(s) 126, 292, 581, 590, 809
HspAI GCGC 1 cut(s) 641
Kzo9I GATC 2 cut(s) 452, 516
LmnI GCTCC 3 cut(s) 359, 744, 761
Lsp1109I GCAGC 2 cut(s) 406, 418
LweI GCATC 2 cut(s) 76, 340
MaeI CTAG 2 cut(s) 528, 779
MaeII ACGT 1 cut(s) 413
MaeIII GTNAC 2 cut(s) 409, 470
MalI GATC 2 cut(s) 454, 518
MbiI CCGCTC 1 cut(s) 764
MboI GATC 2 cut(s) 452, 516
MboII GAAGA 6 cut(s) 96, 99, 287, 313, 511, 761
MflI RGATCY 1 cut(s) 516
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 2 cut(s) 292, 317
MluNI TGGCCA 1 cut(s) 582
MlyI GAGTC 4 cut(s) 23, 271, 335, 711
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 8 cut(s) 121, 154, 224, 249, 252, 374, 485, 668
Mox20I TGGCCA 1 cut(s) 582
Mph1103I ATGCAT 1 cut(s) 376
MscI TGGCCA 1 cut(s) 582
MseI TTAA 3 cut(s) 23, 738, 770
Msp20I TGGCCA 1 cut(s) 582
MspI CCGG 2 cut(s) 193, 672
MspR9I CCNGG 2 cut(s) 512, 759
MvaI CCWGG 2 cut(s) 512, 759
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 4 cut(s) 86, 359, 437, 583
NdeII GATC 2 cut(s) 452, 516
NheI GCTAGC 1 cut(s) 778
NlaIII CATG 5 cut(s) 126, 292, 581, 590, 809
NlaIV GGNNCC 3 cut(s) 336, 634, 656
NsbI TGCGCA 1 cut(s) 642
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 1 cut(s) 590
PceI AGGCCT 1 cut(s) 80
PfeI GAWTC 3 cut(s) 233, 404, 754
PfoI TCCNGGA 1 cut(s) 757
PkrI GCNGC 2 cut(s) 396, 433
PleI GAGTC 4 cut(s) 22, 270, 334, 711
PpsI GAGTC 4 cut(s) 22, 270, 334, 711
PpuMI RGGWCCY 1 cut(s) 335
PshBI ATTAAT 1 cut(s) 770
PsiI TTATAA 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 510, 757
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 2 cut(s) 510, 757
PspN4I GGNNCC 3 cut(s) 336, 634, 656
PspPI GGNCC 2 cut(s) 335, 459
PspPPI RGGWCCY 1 cut(s) 335
PsuI RGATCY 1 cut(s) 516
RsaI GTAC 2 cut(s) 178, 199
RsaNI GTAC 2 cut(s) 177, 198
SacI GAGCTC 1 cut(s) 206
SaqAI TTAA 3 cut(s) 23, 738, 770
SatI GCNGC 2 cut(s) 395, 432
Sau3AI GATC 2 cut(s) 452, 516
Sau96I GGNCC 2 cut(s) 335, 459
ScaI AGTACT 1 cut(s) 178
SchI GAGTC 4 cut(s) 23, 271, 335, 711
ScrFI CCNGG 2 cut(s) 512, 759
SduI GDGCHC 1 cut(s) 206
SfaNI GCATC 2 cut(s) 76, 340
SinI GGWCC 1 cut(s) 335
SmlI CTYRAG 2 cut(s) 95, 112
SmoI CTYRAG 2 cut(s) 95, 112
Sse9I AATT 2 cut(s) 292, 317
SseBI AGGCCT 1 cut(s) 80
SsiI CCGC 3 cut(s) 457, 494, 764
SspMI CTAG 2 cut(s) 528, 779
SstI GAGCTC 1 cut(s) 206
StuI AGGCCT 1 cut(s) 80
StyD4I CCNGG 2 cut(s) 510, 757
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 1 cut(s) 416
TasI AATT 2 cut(s) 292, 317
TatI WGTACW 1 cut(s) 176
TfiI GAWTC 3 cut(s) 233, 404, 754
Tru1I TTAA 3 cut(s) 23, 738, 770
Tru9I TTAA 3 cut(s) 23, 738, 770
TscAI CASTG 2 cut(s) 62, 404
TseI GCWGC 2 cut(s) 394, 431
TspDTI ATGAA 5 cut(s) 81, 288, 305, 617, 794
TspGWI ACGGA 2 cut(s) 465, 697
TspRI CASTG 2 cut(s) 62, 404
VpaK11BI GGWCC 1 cut(s) 335
VspI ATTAAT 1 cut(s) 770
XagI CCTNNNNNAGG 1 cut(s) 218
XapI RAATTY 1 cut(s) 292
XceI RCATGY 1 cut(s) 590
XspI CTAG 2 cut(s) 528, 779
ZrmI AGTACT 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.