FvH4_3g38560

DUF1771

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001145
Physical Location & Seq
Forward (+)
32898935 .. 32904379
5445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38560.t2

Sequence Viewer

Length: 1557 bp
ATGAAGAAGCAGACGAGGAGGAGGAGGAAGAAGACCTCGCCGGCGGATCCGACGCCGGCCGCCGCCCCCAAAGACGACGCCGAGGAGAAGGCCGTCGCCGCGCTGAGGGAGGCTTTCTCGTCTGTCTCGGTTGACGACGCCGTTTCGGCGCTTCATGAAGCCAACGGCGACCCGGACAAGGCCGCGGAGATTTTGGTCGAGTCTCTCGCCGACGCTTCCGACGAGCCGTTCACGAGCACTACGTCCGGCGTTTCCGGCTCCGATACAGGGTCTGATGATCCGTTTACTAGCTCTTCGTCGTCCAGCGGCGTGTCCGGATCTGATACGGGCTCGACTTCCGGGTCGGGTTCGTCGGAGGGGTTTGAGTCGGGTTGTGTTCAGAATTTAGTGAGCGAGAGGTGTAGGAATAAGCAGAAGAGGGTGATTGCGTCGGCCGGAACGGTTTCGAATGTGCTGGGAAAAGAGTATGTGAGGTCTAGGATGACGAAGCCCTCTGGTTCGAAAACTGGAGATGCTGTTGGGGAAGAGGAAGCGGAACAGTTCCTCTGTTCAATGCTTGGTGATGACTCTGAGCTCAACATGGCTGTTATTAGAGATGTGCTTTGTCAGTGTGGGTATGATGTTGAAAAGGCCTTGGATTGCTTACTTGAGGTATCTGCGTCCTTGTCTGAACAGTCCAGCCTTAGTTCTAACTATAGTTTGGTTTATAAAGACGATGGTAGATATCCTGCCGGACAGTTTGACTATCTGACAGATAGGGCATCAGATTGTACTTCTTATTCATCTGAGGATAATATATGGTATTCAGGATACTCTCGCCGGAACTATGCGGAGGTTCTTGCTAGTTCGGAAGCTCATCCCCCCACGAGCCCAAGTACTACCGAATCAAATCTCCCGCAAAAGGTGTTGGAATCTTTGTTTAACATATCTAAGACTCCTGAATATGAACCGAAAGCTATGAATTGGAAGAATGTAGTGAGTAAGTTGCAGTCATTAGGACCTGAATTTGATGGTTGCACGTCAAGCTCTTCAGTAGCAAAACAAGACACTTTGGCTAAAGGAGATGAGTATCATGAATTTAGAGGAACTGCAAGGCAGCACTGGGATACCATGAAGTCTTACTATCAGAAGGCTGCTACAGCATATTCAAACGGATCAAGGGGGCATGCAGGCTATCTTTCTGAGCAGGGGAGGGTACAGACTAAAATGGCACAAAAGGCTGATGAAAAGGCAAGCCAGGAAATCTTCAAAGCTAGAAACAAGGACATAGAAAATGTGATAACAATCGATTTGCATGGGCAGCACGTCAAACAAGCAATGAAGCTTTTAAAAATCCATCTTCTATTTGGAACATATGCTCAATCGGTTCAATTTCTCAGGGTTATCACAGGATGTGGGTCTCATGGTCTAGGAAAGTCAAAGCTGAAACAATCGGTCATACAGCTTGTTGAGAACGAAGGTATCACATGGTCTGAAGAGAATAAGGGGGTTGTATTAATAAAGCTTGGTTCACACACGGAGTTCAACTTTATGGATTCCGAAAGTGATGACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1771 Smr

Protein Analysis

519

Amino Acids

56.15

Weight (kDa)

5.16

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_At5g58720 PF24767 26 - 67 1.5e-16 At5g58720 UBA-like domain
DUF1771 PF08590 360 - 423 4.6e-18 Domain of unknown function (DUF1771)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 708
AasI GACNNNNNNGTC 1 cut(s) 340
AccII CGCG 2 cut(s) 101, 185
AccIII TCCGGA 1 cut(s) 314
AclWI GGATC 5 cut(s) 41, 54, 272, 325, 1162
AcoI YGGCCR 2 cut(s) 57, 432
AcsI RAATTY 3 cut(s) 382, 1004, 1076
AcuI CTGAAG 2 cut(s) 1014, 1494
AcyI GRCGYC 3 cut(s) 53, 78, 138
AfaI GTAC 3 cut(s) 772, 877, 1197
AfiI CCNNNNNNNGG 4 cut(s) 105, 178, 267, 901
AgsI TTSAA 6 cut(s) 552, 626, 1149, 1249, 1370, 1525
AjiI CACGTC 2 cut(s) 1020, 1306
AjnI CCWGG 1 cut(s) 1236
Alw21I GWGCWC 2 cut(s) 239, 576
Alw26I GTCTC 3 cut(s) 130, 207, 1404
AlwI GGATC 5 cut(s) 41, 54, 272, 325, 1162
AlwNI CAGNNNCTG 1 cut(s) 272
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 5 cut(s) 57, 90, 180, 432, 630
ApeKI GCWGC 3 cut(s) 1096, 1133, 1300
ApoI RAATTY 3 cut(s) 382, 1004, 1076
AseI ATTAAT 1 cut(s) 1496
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 2 cut(s) 103, 151
AspS9I GGNCC 1 cut(s) 998
AsuC2I CCSGG 2 cut(s) 173, 340
AsuHPI GGTGA 2 cut(s) 433, 572
AsuII TTCGAA 2 cut(s) 446, 500
AvaII GGWCC 1 cut(s) 998
BaeI ACNNNNGTAYC 2 cut(s) 1444, 1477
BamHI GGATCC 1 cut(s) 46
BanII GRGCYC 3 cut(s) 332, 576, 872
BauI CACGAG 2 cut(s) 232, 865
BbsI GAAGAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 239, 576
BbvCI CCTCAGC 1 cut(s) 104
BbvI GCAGC 3 cut(s) 1108, 1120, 1312
BccI CCATC 3 cut(s) 710, 1004, 1344
BceAI ACGGC 4 cut(s) 77, 125, 181, 211
BciT130I CCWGG 1 cut(s) 1238
BciVI GTATCC 2 cut(s) 803, 1099
BcnI CCSGG 2 cut(s) 173, 340
BcoDI GTCTC 3 cut(s) 130, 207, 1404
BfaI CTAG 5 cut(s) 288, 477, 843, 1254, 1409
BfmI CTRYAG 2 cut(s) 694, 1137
BfoI RGCGCY 1 cut(s) 152
BfuI GTATCC 2 cut(s) 803, 1099
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 8 cut(s) 60, 63, 99, 183, 307, 1097, 1134, 1301
BlsI GCNGC 8 cut(s) 61, 64, 100, 184, 308, 1098, 1135, 1302
BmcAI AGTACT 1 cut(s) 877
Bme1390I CCNGG 3 cut(s) 173, 340, 1238
Bme18I GGWCC 1 cut(s) 998
BmgBI CACGTC 2 cut(s) 1020, 1306
BmgT120I GGNCC 1 cut(s) 998
BmiI GGNNCC 2 cut(s) 48, 259
BmrFI CCNGG 3 cut(s) 173, 340, 1238
BmrI ACTGGG 1 cut(s) 1111
BmsI GCATC 2 cut(s) 502, 770
BmuI ACTGGG 1 cut(s) 1111
BpiI GAAGAC 1 cut(s) 38
BplI GAGNNNNNCTC 2 cut(s) 101, 133
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 104
Bpu14I TTCGAA 2 cut(s) 446, 500
BpuEI CTTGAG 1 cut(s) 668
BpuMI CCSGG 2 cut(s) 173, 340
Bsa29I ATCGAT 1 cut(s) 1287
BsaBI GATNNNNATC 2 cut(s) 1068, 1283
BsaHI GRCGYC 3 cut(s) 53, 78, 138
BsaI GGTCTC 1 cut(s) 1404
BsaJI CCNNGG 3 cut(s) 81, 183, 633
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 178, 267, 901
Bse118I RCCGGY 2 cut(s) 40, 55
Bse1I ACTGG 2 cut(s) 511, 1106
Bse3DI GCAATG 1 cut(s) 1323
Bse8I GATNNNNATC 2 cut(s) 1068, 1283
BseAI TCCGGA 1 cut(s) 314
BseBI CCWGG 1 cut(s) 1238
BseCI ATCGAT 1 cut(s) 1287
BseDI CCNNGG 3 cut(s) 81, 183, 633
BseGI GGATG 3 cut(s) 486, 856, 1397
BseJI GATNNNNATC 2 cut(s) 1068, 1283
BseLI CCNNNNNNNGG 4 cut(s) 105, 178, 267, 901
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 5 cut(s) 95, 561, 777, 1173, 1390
BseNI ACTGG 2 cut(s) 511, 1106
BseRI GAGGAG 4 cut(s) 31, 34, 37, 98
BseX3I CGGCCG 2 cut(s) 57, 432
BseXI GCAGC 3 cut(s) 1108, 1120, 1312
BseYI CCCAGC 1 cut(s) 454
Bsh1236I CGCG 2 cut(s) 101, 185
Bsh1285I CGRYCG 2 cut(s) 60, 435
BshFI GGCC 5 cut(s) 59, 92, 182, 434, 632
BshVI ATCGAT 1 cut(s) 1287
BsiEI CGRYCG 2 cut(s) 60, 435
BsiHKAI GWGCWC 2 cut(s) 239, 576
BslI CCNNNNNNNGG 4 cut(s) 105, 178, 267, 901
BsmAI GTCTC 3 cut(s) 130, 207, 1404
BsnI GGCC 5 cut(s) 59, 92, 182, 434, 632
Bso31I GGTCTC 1 cut(s) 1404
Bsp119I TTCGAA 2 cut(s) 446, 500
Bsp1286I GDGCHC 4 cut(s) 239, 332, 576, 872
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 4 cut(s) 46, 277, 317, 1154
BspANI GGCC 5 cut(s) 59, 92, 182, 434, 632
BspCNI CTCAG 5 cut(s) 96, 562, 778, 1174, 1389
BspDI ATCGAT 1 cut(s) 1287
BspEI TCCGGA 1 cut(s) 314
BspFNI CGCG 2 cut(s) 101, 185
BspHI TCATGA 2 cut(s) 154, 1072
BspLI GGNNCC 2 cut(s) 48, 259
BspPI GGATC 5 cut(s) 41, 54, 272, 325, 1162
BspQI GCTCTTC 2 cut(s) 298, 1033
BspT104I TTCGAA 2 cut(s) 446, 500
BspTNI GGTCTC 1 cut(s) 1404
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 2 cut(s) 40, 55
BsrI ACTGG 2 cut(s) 511, 1106
BssAI RCCGGY 2 cut(s) 40, 55
BssECI CCNNGG 3 cut(s) 81, 183, 633
BssMI GATC 4 cut(s) 46, 277, 317, 1154
BssNI GRCGYC 3 cut(s) 53, 78, 138
BssSI CACGAG 2 cut(s) 232, 865
BssT1I CCWWGG 1 cut(s) 633
Bst2BI CACGAG 2 cut(s) 232, 865
Bst2UI CCWGG 1 cut(s) 1238
Bst4CI ACNGT 4 cut(s) 442, 540, 675, 738
Bst6I CTCTTC 5 cut(s) 298, 410, 519, 1033, 1470
BstACI GRCGYC 3 cut(s) 53, 78, 138
BstBI TTCGAA 2 cut(s) 446, 500
BstC8I GCNNGC 5 cut(s) 42, 57, 1167, 1171, 1234
BstDEI CTNAG 7 cut(s) 104, 570, 683, 786, 930, 1182, 1376
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 3 cut(s) 486, 856, 1397
BstFNI CGCG 2 cut(s) 101, 185
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 103, 151
BstKTI GATC 4 cut(s) 49, 280, 320, 1157
BstMAI GTCTC 3 cut(s) 130, 207, 1404
BstMBI GATC 4 cut(s) 46, 277, 317, 1154
BstMCI CGRYCG 2 cut(s) 60, 435
BstMWI GCNNNNNNNGC 7 cut(s) 98, 146, 255, 1023, 1139, 1217, 1300
BstNI CCWGG 1 cut(s) 1238
BstNSI RCATGY 1 cut(s) 1169
BstSCI CCNGG 3 cut(s) 171, 338, 1236
BstSFI CTRYAG 2 cut(s) 694, 1137
BstUI CGCG 2 cut(s) 101, 185
BstV1I GCAGC 3 cut(s) 1108, 1120, 1312
BstV2I GAAGAC 1 cut(s) 38
BstX2I RGATCY 2 cut(s) 46, 317
BstYI RGATCY 2 cut(s) 46, 317
BstZI CGGCCG 2 cut(s) 57, 432
Bsu15I ATCGAT 1 cut(s) 1287
BsuI GTATCC 2 cut(s) 803, 1099
BsuRI GGCC 5 cut(s) 59, 92, 182, 434, 632
BsuTUI ATCGAT 1 cut(s) 1287
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 2 cut(s) 1020, 1306
BtsCI GGATG 3 cut(s) 486, 856, 1397
BtsIMutI CAGTG 2 cut(s) 614, 1099
Cac8I GCNNGC 5 cut(s) 42, 57, 1167, 1171, 1234
CaiI CAGNNNCTG 1 cut(s) 272
CciI TCATGA 2 cut(s) 154, 1072
CfoI GCGC 2 cut(s) 103, 151
Cfr10I RCCGGY 2 cut(s) 40, 55
Cfr13I GGNCC 1 cut(s) 998
Cfr42I CCGCGG 1 cut(s) 186
ClaI ATCGAT 1 cut(s) 1287
CseI GACGC 6 cut(s) 61, 86, 146, 221, 417, 648
Csp6I GTAC 3 cut(s) 771, 876, 1196
CviAII CATG 8 cut(s) 155, 580, 1073, 1111, 1166, 1295, 1403, 1467
CviQI GTAC 3 cut(s) 771, 876, 1196
DdeI CTNAG 7 cut(s) 104, 570, 683, 786, 930, 1182, 1376
DpnI GATC 4 cut(s) 48, 279, 319, 1156
DpnII GATC 4 cut(s) 46, 277, 317, 1154
DraI TTTAAA 1 cut(s) 1329
DrdI GACNNNNNNGTC 1 cut(s) 340
DseDI GACNNNNNNGTC 1 cut(s) 340
EaeI YGGCCR 2 cut(s) 57, 432
EagI CGGCCG 2 cut(s) 57, 432
Eam1104I CTCTTC 5 cut(s) 298, 410, 519, 1033, 1470
EarI CTCTTC 5 cut(s) 298, 410, 519, 1033, 1470
EciI GGCGGA 1 cut(s) 59
Ecl136II GAGCTC 1 cut(s) 574
EclXI CGGCCG 2 cut(s) 57, 432
Eco130I CCWWGG 1 cut(s) 633
Eco147I AGGCCT 1 cut(s) 632
Eco24I GRGCYC 3 cut(s) 332, 576, 872
Eco31I GGTCTC 1 cut(s) 1404
Eco32I GATATC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 998
Eco52I CGGCCG 2 cut(s) 57, 432
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 2 cut(s) 1014, 1494
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 998
EcoRII CCWGG 1 cut(s) 1236
EcoRV GATATC 1 cut(s) 725
EcoT14I CCWWGG 1 cut(s) 633
EcoT38I GRGCYC 3 cut(s) 332, 576, 872
ErhI CCWWGG 1 cut(s) 633
FaeI CATG 8 cut(s) 158, 583, 1076, 1114, 1169, 1298, 1406, 1470
FatI CATG 8 cut(s) 154, 579, 1072, 1110, 1165, 1294, 1402, 1466
FauI CCCGC 1 cut(s) 903
FauNDI CATATG 1 cut(s) 1354
Fnu4HI GCNGC 8 cut(s) 60, 63, 99, 183, 307, 1097, 1134, 1301
FokI GGATG 3 cut(s) 493, 843, 1404
FriOI GRGCYC 3 cut(s) 332, 576, 872
Fsp4HI GCNGC 8 cut(s) 60, 63, 99, 183, 307, 1097, 1134, 1301
FspBI CTAG 5 cut(s) 288, 477, 843, 1254, 1409
GlaI GCGC 2 cut(s) 102, 150
GluI GCNGC 8 cut(s) 60, 63, 99, 183, 307, 1097, 1134, 1301
GsaI CCCAGC 1 cut(s) 458
GsuI CTGGAG 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 5 cut(s) 59, 92, 182, 434, 632
HgaI GACGC 6 cut(s) 61, 86, 146, 221, 417, 648
HhaI GCGC 2 cut(s) 103, 151
Hin1I GRCGYC 3 cut(s) 53, 78, 138
Hin1II CATG 8 cut(s) 158, 583, 1076, 1114, 1169, 1298, 1406, 1470
Hin6I GCGC 2 cut(s) 101, 149
HinP1I GCGC 2 cut(s) 101, 149
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HindIII AAGCTT 2 cut(s) 1322, 1502
HinfI GANTC 7 cut(s) 200, 365, 566, 884, 911, 934, 1535
HphI GGTGA 2 cut(s) 433, 572
Hpy166II GTNNAC 4 cut(s) 133, 231, 285, 1511
Hpy188III TCNNGA 6 cut(s) 155, 232, 315, 807, 938, 1073
Hpy8I GTNNAC 4 cut(s) 133, 231, 285, 1511
Hpy99I CGWCG 9 cut(s) 55, 80, 98, 140, 215, 224, 301, 355, 433
HpyAV CCTTC 3 cut(s) 82, 1123, 1451
HpyCH4III ACNGT 4 cut(s) 442, 540, 675, 738
HpyCH4IV ACGT 3 cut(s) 242, 1019, 1305
HpyCH4V TGCA 5 cut(s) 988, 1017, 1091, 1169, 1294
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 146, 255, 1023, 1139, 1217, 1300
HpyF3I CTNAG 7 cut(s) 104, 570, 683, 786, 930, 1182, 1376
HpySE526I ACGT 3 cut(s) 242, 1019, 1305
Hsp92I GRCGYC 3 cut(s) 53, 78, 138
Hsp92II CATG 8 cut(s) 158, 583, 1076, 1114, 1169, 1298, 1406, 1470
HspAI GCGC 2 cut(s) 101, 149
Kpn2I TCCGGA 1 cut(s) 314
KroI GCCGGC 2 cut(s) 40, 55
KroNI GCCGGC 2 cut(s) 42, 57
KspI CCGCGG 1 cut(s) 186
Kzo9I GATC 4 cut(s) 46, 277, 317, 1154
LguI GCTCTTC 2 cut(s) 298, 1033
LmnI GCTCC 1 cut(s) 263
Lsp1109I GCAGC 3 cut(s) 1108, 1120, 1312
LweI GCATC 2 cut(s) 502, 770
MaeI CTAG 5 cut(s) 288, 477, 843, 1254, 1409
MaeII ACGT 3 cut(s) 242, 1019, 1305
MalI GATC 4 cut(s) 48, 279, 319, 1156
MboI GATC 4 cut(s) 46, 277, 317, 1154
MflI RGATCY 2 cut(s) 46, 317
MhlI GDGCHC 4 cut(s) 239, 332, 576, 872
MluCI AATT 5 cut(s) 382, 961, 1004, 1076, 1370
MlyI GAGTC 4 cut(s) 209, 374, 560, 928
MmeI TCCRAC 4 cut(s) 74, 243, 333, 888
MreI CGCCGGCG 1 cut(s) 40
MroI TCCGGA 1 cut(s) 314
MroNI GCCGGC 2 cut(s) 40, 55
MroXI GAANNNNTTC 1 cut(s) 442
MseI TTAA 3 cut(s) 921, 1328, 1496
MspA1I CMGCKG 2 cut(s) 185, 306
MspR9I CCNGG 3 cut(s) 173, 340, 1238
MvaI CCWGG 1 cut(s) 1238
MvnI CGCG 2 cut(s) 101, 185
MwoI GCNNNNNNNGC 7 cut(s) 98, 146, 255, 1023, 1139, 1217, 1300
NaeI GCCGGC 2 cut(s) 42, 57
NciI CCSGG 2 cut(s) 173, 340
NdeI CATATG 1 cut(s) 1354
NdeII GATC 4 cut(s) 46, 277, 317, 1154
NgoMIV GCCGGC 2 cut(s) 40, 55
NlaIII CATG 8 cut(s) 158, 583, 1076, 1114, 1169, 1298, 1406, 1470
NlaIV GGNNCC 2 cut(s) 48, 259
NmeAIII GCCGAG 1 cut(s) 106
NspI RCATGY 1 cut(s) 1169
NspV TTCGAA 2 cut(s) 446, 500
PaeI GCATGC 1 cut(s) 1169
PagI TCATGA 2 cut(s) 154, 1072
PceI AGGCCT 1 cut(s) 632
PciSI GCTCTTC 2 cut(s) 298, 1033
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 239
PdiI GCCGGC 2 cut(s) 42, 57
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 3 cut(s) 884, 911, 1535
PkrI GCNGC 8 cut(s) 61, 64, 100, 184, 308, 1098, 1135, 1302
PleI GAGTC 4 cut(s) 208, 373, 560, 928
PpsI GAGTC 4 cut(s) 208, 373, 560, 928
PpuMI RGGWCCY 1 cut(s) 998
PshBI ATTAAT 1 cut(s) 1496
PsiI TTATAA 1 cut(s) 708
Psp124BI GAGCTC 1 cut(s) 576
Psp5II RGGWCCY 1 cut(s) 998
Psp6I CCWGG 1 cut(s) 1236
PspFI CCCAGC 1 cut(s) 454
PspGI CCWGG 1 cut(s) 1236
PspN4I GGNNCC 2 cut(s) 48, 259
PspPI GGNCC 1 cut(s) 998
PspPPI RGGWCCY 1 cut(s) 998
PstNI CAGNNNCTG 1 cut(s) 272
PsuI RGATCY 2 cut(s) 46, 317
RsaI GTAC 3 cut(s) 772, 877, 1197
RsaNI GTAC 3 cut(s) 771, 876, 1196
SacI GAGCTC 1 cut(s) 576
SacII CCGCGG 1 cut(s) 186
SapI GCTCTTC 2 cut(s) 298, 1033
SaqAI TTAA 3 cut(s) 921, 1328, 1496
SatI GCNGC 8 cut(s) 60, 63, 99, 183, 307, 1097, 1134, 1301
Sau3AI GATC 4 cut(s) 46, 277, 317, 1154
Sau96I GGNCC 1 cut(s) 998
ScaI AGTACT 1 cut(s) 877
SchI GAGTC 4 cut(s) 209, 374, 560, 928
ScrFI CCNGG 3 cut(s) 173, 340, 1238
SduI GDGCHC 4 cut(s) 239, 332, 576, 872
SfaNI GCATC 2 cut(s) 502, 770
SfcI CTRYAG 2 cut(s) 694, 1137
Sfr303I CCGCGG 1 cut(s) 186
SfuI TTCGAA 2 cut(s) 446, 500
SgrAI CRCCGGYG 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 186
SinI GGWCC 1 cut(s) 998
SmlI CTYRAG 1 cut(s) 647
SmoI CTYRAG 1 cut(s) 647
SphI GCATGC 1 cut(s) 1169
Sse9I AATT 5 cut(s) 382, 961, 1004, 1076, 1370
SseBI AGGCCT 1 cut(s) 632
SspMI CTAG 5 cut(s) 288, 477, 843, 1254, 1409
SstI GAGCTC 1 cut(s) 576
StuI AGGCCT 1 cut(s) 632
StyD4I CCNGG 3 cut(s) 171, 338, 1236
StyI CCWWGG 1 cut(s) 633
TaaI ACNGT 4 cut(s) 442, 540, 675, 738
TaiI ACGT 3 cut(s) 245, 1022, 1308
TaqI TCGA 5 cut(s) 198, 332, 446, 500, 1287
TaqII GACCGA 1 cut(s) 1423
TasI AATT 5 cut(s) 382, 961, 1004, 1076, 1370
TatI WGTACW 2 cut(s) 770, 875
TauI GCSGC 5 cut(s) 62, 65, 101, 185, 309
TfiI GAWTC 3 cut(s) 884, 911, 1535
Tru1I TTAA 3 cut(s) 921, 1328, 1496
Tru9I TTAA 3 cut(s) 921, 1328, 1496
TscAI CASTG 2 cut(s) 614, 1106
TseI GCWGC 3 cut(s) 1096, 1133, 1300
TspGWI ACGGA 3 cut(s) 270, 1167, 1532
TspRI CASTG 2 cut(s) 614, 1106
VpaK11BI GGWCC 1 cut(s) 998
VspI ATTAAT 1 cut(s) 1496
XapI RAATTY 3 cut(s) 382, 1004, 1076
XceI RCATGY 1 cut(s) 1169
XcmI CCANNNNNNNNNTGG 1 cut(s) 1343
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 5 cut(s) 288, 477, 843, 1254, 1409
ZrmI AGTACT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.