FvH4_1g25960

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001142
Physical Location & Seq
Reverse (-)
17835248 .. 17847519
12272 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25960.t1

Sequence Viewer

Length: 2367 bp
ATGGATAACGGTTACGATCGGAAATTCGCAGACAAGTTCTCGGGGCTGGCGATTTCCGGCGCCGGTGATCCTATTGGTGGAGCGAACAACAACCACGGCAACAATGATAGCAGCTTGTTTCAGGTCATAAAGGCGGTGGAAGCTGCGGAGGCAACGATTAAGCAACAGGTGGAGGAGAACATCCGATTGAGGACTGAGCTTCAGAACAAGATTCTTGAGCTGGATAGATTTAAAGTAGAGGAATCTGGTGCGCAAAGGCCGCGTGCCGATGATCCTTGGTTTGAGCGGGTCCAAGGATCGTATGAAGCTCATCAACCAGTTCCCCCTGTCGGTAGTCAAGAAGATAGGAATAGGAGTGCGGTCAATACTTCTGGACTTGATCCATCTGGTAGACTAGTTCTACACCAAGAGAGTTTGAAGCCAAATGCTGATGCTCCTCTACAAAGTAATGCGGAAACCCAGTCCTCAAGCAGCAAGATCAATGGGACCATGAAGGAACTTCCGGGTAGTCAGCAGCCTGTGGATAATGCCGGCTTCTCTCACTTATCTTCACCATCCACAACATCCTTCTCTCCGGGAAGGTTTAACACACAAGGAGAATATGATCCAAGGTTCCCTTTTTCTGGACAAGGGTTGATGCCTATAACTGAATTAAATCCTAGAAGCCTCTGGAAGCAGGATCTTATTGTCAAGACTCGTGAGCATGAAGATGAGATCATGCAATTACGGAGACATCTGTCTGATTATTCCATAAAGGAAGCACAAATACGCAACGAAAAATATGTCCTTGAGAAGCGCATTGCTTATATGCGTCTGGCCTTTGATCAACAGCAACAGGATCTTGTAGATGCTGCATCGAAAGCTCTATCTTACCGACAAGACATAATTGAGGAAAATATACGCCTCACATATGCATTACAGGATGCACAGCAAGAGAGATCGACATTTGTTTCATCCTTGCTCCCACTTCTTGCAGAGTACTCTTTGCAGCCTCTAGTTCTTGATGCCCAATCAATTGTCAGCAATGTCAAGGTTCTTTTTAAACATTTGCAAGAAAAGCTCCTTCTTACTGAGGCAAAACTAAAGGAGTCACAGTATCATGTACGACCCTGGAACTCAGAGTTGAATAACTCTAACATTCCCATGCAGTCGCCTTCACGCTCTTTTGGTGCAGCATTGACAACTACTCACAAAAATGGGCTTGAACTAGTTCCTCAGCAAACATATTCCCAGGGAAAAGTACCAGTTTCAGCTTCGGATGTTCAGACCACCAGGGATTGGGATCTATTGGGTCATGATCAGAGTGCTTTGGATGGTGTTGTCAGTAGGAATGTGGAACCCAATGACCTGGGGAGGTATTCACCTTTTGCTAGCAGGAATTCTGCAGCACGAGATACACCTACTCAGCTTGATGTTATCCGCGGTGATGCACCAATAACTCGTTATAGTGAAGAAACCACCAATAAACAGGTCACATTTCGTGATCCTGTTAGGAACACAGAGCTCGATGATCAAGATCCTGAGGGAAACCAACATGAGAGAGAAATGTCAACAAATTGGAGTCCAGGAAACACTCCTTACACAACTGCACCTGATGAGCCCGGTTCCTCGTATCATTTTCTACCGCCAGTTCTTGAAGAGCCGTCTTCTTCATTTTCTGAGGCTGCAGATGATGATCCATTACCAGCTATTGAGAACCTTCAAATTTCAGGAGAAGCTTTTCCAGGGAGAGAACTTCAAGCATGCGGGTACTCTATTAATGGGACAACCAGTTGCAATTTTGAGTGGGTACGCCATATGGAAGATGGATCAGTTAATTATATCGAAGGAGCAAAGCAACCAAACTATCTAATTACTGCGGATGATGTTGATACATATCTTGCTATTGAAGTCCAGCCTCTTGATAACAGGAAGCGCAAGGGAGAGCTTGTAAAAGTTTTTGCGAATGAGAACAGAAAGATTCCTTGTGATCCTGAAATGCAAGCCGAGTTACAGAAGATTCTTAATCAAGGTCATTCTTCATTTAAAGTATATCAGTCGACGGGATATCTTGATATATGGGAACCTGCTATATTGGCCATTAAGAAGGACAGTTACAGCATTAAATGTACCGGGCCCACTGGTACTGTAATCACTGAAAAGTTTTCGCCGACAACTAGTGTTGGAATCCCATATGGAAGCCCTACAGAGTTCGTGCTAACTGATTCTGGTGGTGGTCAGCGTCTCCTAAAAACCGACAATGACCAGGCAGATTATAGCTGTTCTAGAGATACAGTTGTGCTCACATTGAGAATGTTTATCCTAAAGGGACTTTGTGGTGTACTGGTGTGTAATTTTCTTTTGACTTCCCTTCTAGAAGGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

789

Amino Acids

87.74

Weight (kDa)

5.01

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IG_AIR9 PF23197 564 - 646 4.7e-08 AIR9 A9 domain
DUF7046 PF23080 679 - 770 2.7e-31 Domain of unknown function (DUF7046)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 2074
AccB1I GGYRCC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 1278
AccBSI CCGCTC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 391, 2039
AccII CGCG 2 cut(s) 262, 1422
AcoI YGGCCR 1 cut(s) 2076
AcsI RAATTY 3 cut(s) 23, 1378, 1704
AcuI CTGAAG 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 8 cut(s) 980, 1104, 1242, 1751, 1791, 2110, 2125, 2322
AfiI CCNNNNNNNGG 4 cut(s) 77, 329, 623, 1278
AgsI TTSAA 7 cut(s) 418, 1126, 1205, 1637, 1703, 1739, 1889
AhlI ACTAGT 3 cut(s) 394, 1207, 2156
AjnI CCWGG 7 cut(s) 1109, 1230, 1271, 1347, 1564, 1723, 2244
Alw21I GWGCWC 2 cut(s) 1506, 2283
Alw26I GTCTC 2 cut(s) 724, 2228
Ama87I CYCGRG 1 cut(s) 40
AoxI GGCC 4 cut(s) 257, 816, 2076, 2114
ApaI GGGCCC 1 cut(s) 2118
ApeKI GCWGC 9 cut(s) 111, 143, 471, 514, 851, 988, 1172, 1385, 1664
ApoI RAATTY 3 cut(s) 23, 1378, 1704
ArsI GACNNNNNNTTYG 2 cut(s) 768, 800
AseI ATTAAT 1 cut(s) 1758
Asp700I GAANNNNTTC 2 cut(s) 1209, 1719
AspLEI GCGC 4 cut(s) 62, 253, 798, 1917
AspS9I GGNCC 4 cut(s) 289, 486, 2114, 2115
AsuC2I CCSGG 4 cut(s) 504, 576, 1602, 2113
AsuHPI GGTGA 4 cut(s) 77, 543, 1353, 1436
AsuNHI GCTAGC 1 cut(s) 1370
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 289, 486
AxyI CCTNAGG 1 cut(s) 1521
BaeGI GKGCMC 1 cut(s) 2118
BaeI ACNNNNGTAYC 2 cut(s) 2262, 2295
BalI TGGCCA 1 cut(s) 2078
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 3 cut(s) 1506, 1602, 2118
BarI GAAGNNNNNNTAC 4 cut(s) 750, 782, 2078, 2110
BauI CACGAG 2 cut(s) 696, 1389
BbsI GAAGAC 1 cut(s) 1638
Bbv12I GWGCWC 2 cut(s) 1506, 2283
BbvCI CCTCAGC 1 cut(s) 1215
BbvI GCAGC 9 cut(s) 123, 130, 483, 526, 838, 1000, 1184, 1397, 1651
BccI CCATC 4 cut(s) 391, 562, 1307, 1799
BceAI ACGGC 2 cut(s) 112, 1627
BciT130I CCWGG 7 cut(s) 1111, 1232, 1273, 1349, 1566, 1725, 2246
BclI TGATCA 3 cut(s) 823, 1297, 1510
BcnI CCSGG 4 cut(s) 504, 576, 1602, 2113
BcoDI GTCTC 2 cut(s) 724, 2228
BcuI ACTAGT 3 cut(s) 394, 1207, 2156
BfaI CTAG 8 cut(s) 395, 660, 995, 1208, 1371, 2157, 2265, 2354
BfmI CTRYAG 3 cut(s) 1383, 1665, 2184
BfoI RGCGCY 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 2074
BmcAI AGTACT 1 cut(s) 980
Bme18I GGWCC 2 cut(s) 289, 486
BmeT110I CYCGRG 1 cut(s) 40
BmgT120I GGNCC 4 cut(s) 289, 486, 2114, 2115
BmiI GGNNCC 8 cut(s) 61, 290, 487, 614, 1338, 1606, 2064, 2116
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 7 cut(s) 421, 627, 838, 863, 913, 994, 1417
BmtI GCTAGC 1 cut(s) 1374
BmuI ACTGGG 1 cut(s) 454
BoxI GACNNNNGTC 1 cut(s) 736
BpiI GAAGAC 1 cut(s) 1638
Bpu10I CCTNAGC 1 cut(s) 1215
BpuEI CTTGAG 3 cut(s) 236, 451, 809
BpuMI CCSGG 4 cut(s) 504, 576, 1602, 2113
BsaBI GATNNNNATC 4 cut(s) 1281, 1515, 1674, 1875
BsaHI GRCGYC 1 cut(s) 60
BsaXI ACNNNNNCTCC 4 cut(s) 1079, 1109, 1914, 1944
Bsc4I CCNNNNNNNGG 4 cut(s) 77, 329, 623, 1278
Bse118I RCCGGY 2 cut(s) 62, 530
Bse1I ACTGG 7 cut(s) 317, 460, 1244, 1628, 1770, 2125, 2328
Bse21I CCTNAGG 1 cut(s) 1521
Bse3DI GCAATG 2 cut(s) 798, 1030
Bse8I GATNNNNATC 4 cut(s) 1281, 1515, 1674, 1875
BseBI CCWGG 7 cut(s) 1111, 1232, 1273, 1349, 1566, 1725, 2246
BseGI GGATG 8 cut(s) 180, 554, 563, 928, 953, 1264, 1318, 1867
BseJI GATNNNNATC 4 cut(s) 1281, 1515, 1674, 1875
BseLI CCNNNNNNNGG 4 cut(s) 77, 329, 623, 1278
BseMI GCAATG 2 cut(s) 798, 1030
BseMII CTCAG 7 cut(s) 186, 1062, 1131, 1229, 1418, 1512, 1650
BseNI ACTGG 7 cut(s) 317, 460, 1244, 1628, 1770, 2125, 2328
BseRI GAGGAG 2 cut(s) 188, 426
BseSI GKGCMC 1 cut(s) 2118
BseXI GCAGC 9 cut(s) 123, 130, 483, 526, 838, 1000, 1184, 1397, 1651
BsgI GTGCAG 2 cut(s) 1191, 1572
Bsh1236I CGCG 2 cut(s) 262, 1422
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 4 cut(s) 259, 818, 2078, 2116
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 2 cut(s) 1506, 2283
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 7 cut(s) 57, 63, 503, 531, 575, 1602, 2112
BslFI GGGAC 3 cut(s) 499, 1777, 2322
BslI CCNNNNNNNGG 4 cut(s) 77, 329, 623, 1278
BsmAI GTCTC 2 cut(s) 724, 2228
BsmBI CGTCTC 1 cut(s) 2228
BsmFI GGGAC 3 cut(s) 499, 1777, 2322
BsnI GGCC 4 cut(s) 259, 818, 2078, 2116
BsoBI CYCGRG 1 cut(s) 40
Bsp120I GGGCCC 1 cut(s) 2114
Bsp1286I GDGCHC 4 cut(s) 1506, 1602, 2118, 2283
BspANI GGCC 4 cut(s) 259, 818, 2078, 2116
BspCNI CTCAG 7 cut(s) 187, 1063, 1130, 1228, 1417, 1513, 1651
BspFNI CGCG 2 cut(s) 262, 1422
BspHI TCATGA 1 cut(s) 1294
BspLI GGNNCC 8 cut(s) 61, 290, 487, 614, 1338, 1606, 2064, 2116
BspMAI CTGCAG 2 cut(s) 1387, 1669
BspMI ACCTGC 1 cut(s) 2074
BspOI GCTAGC 1 cut(s) 1374
BspQI GCTCTTC 1 cut(s) 1632
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 2 cut(s) 798, 1030
BsrFI RCCGGY 2 cut(s) 62, 530
BsrI ACTGG 7 cut(s) 317, 460, 1244, 1628, 1770, 2125, 2328
BssAI RCCGGY 2 cut(s) 62, 530
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 2 cut(s) 696, 1389
BssT1I CCWWGG 3 cut(s) 275, 292, 608
Bst2BI CACGAG 2 cut(s) 696, 1389
Bst2UI CCWGG 7 cut(s) 1111, 1232, 1273, 1349, 1566, 1725, 2246
Bst4CI ACNGT 5 cut(s) 11, 1095, 2093, 2128, 2275
Bst6I CTCTTC 1 cut(s) 1632
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 6 cut(s) 48, 264, 532, 1372, 1744, 1983
BstDEI CTNAG 7 cut(s) 195, 1071, 1117, 1215, 1404, 1521, 1659
BstDSI CCRYGG 2 cut(s) 94, 1420
BstF5I GGATG 8 cut(s) 180, 554, 563, 928, 953, 1264, 1318, 1867
BstFNI CGCG 2 cut(s) 262, 1422
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 4 cut(s) 62, 253, 798, 1917
BstMAI GTCTC 2 cut(s) 724, 2228
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 6 cut(s) 140, 149, 259, 860, 1057, 2075
BstNI CCWGG 7 cut(s) 1111, 1232, 1273, 1349, 1566, 1725, 2246
BstNSI RCATGY 1 cut(s) 1746
BstPAI GACNNNNGTC 1 cut(s) 736
BstSFI CTRYAG 3 cut(s) 1383, 1665, 2184
BstSLI GKGCMC 1 cut(s) 2118
BstUI CGCG 2 cut(s) 262, 1422
BstV1I GCAGC 9 cut(s) 123, 130, 483, 526, 838, 1000, 1184, 1397, 1651
BstV2I GAAGAC 1 cut(s) 1638
BstX2I RGATCY 4 cut(s) 679, 838, 1282, 1516
BstXI CCANNNNNNTGG 1 cut(s) 1348
BstYI RGATCY 4 cut(s) 679, 838, 1282, 1516
Bsu36I CCTNAGG 1 cut(s) 1521
BsuRI GGCC 4 cut(s) 259, 818, 2078, 2116
BtgI CCRYGG 2 cut(s) 94, 1420
BtsCI GGATG 8 cut(s) 180, 554, 563, 928, 953, 1264, 1318, 1867
BtsIMutI CAGTG 2 cut(s) 2118, 2133
BveI ACCTGC 1 cut(s) 2074
Cac8I GCNNGC 6 cut(s) 48, 264, 532, 1372, 1744, 1983
CciI TCATGA 1 cut(s) 1294
CfoI GCGC 4 cut(s) 62, 253, 798, 1917
Cfr10I RCCGGY 2 cut(s) 62, 530
Cfr13I GGNCC 4 cut(s) 289, 486, 2114, 2115
Cfr42I CCGCGG 1 cut(s) 1423
CseI GACGC 2 cut(s) 800, 2210
Csp6I GTAC 8 cut(s) 979, 1103, 1241, 1750, 1790, 2109, 2124, 2321
CviAII CATG 8 cut(s) 490, 704, 718, 1100, 1144, 1295, 1535, 1743
CviQI GTAC 8 cut(s) 979, 1103, 1241, 1750, 1790, 2109, 2124, 2321
DdeI CTNAG 7 cut(s) 195, 1071, 1117, 1215, 1404, 1521, 1659
DinI GGCGCC 1 cut(s) 61
DraI TTTAAA 3 cut(s) 232, 1042, 2026
EaeI YGGCCR 1 cut(s) 2076
Eam1104I CTCTTC 1 cut(s) 1632
EarI CTCTTC 1 cut(s) 1632
Ecl136II GAGCTC 1 cut(s) 1504
Eco130I CCWWGG 3 cut(s) 275, 292, 608
Eco24I GRGCYC 3 cut(s) 1506, 1602, 2118
Eco32I GATATC 1 cut(s) 2048
Eco47I GGWCC 2 cut(s) 289, 486
Eco53kI GAGCTC 1 cut(s) 1504
Eco57I CTGAAG 1 cut(s) 185
Eco81I CCTNAGG 1 cut(s) 1521
Eco88I CYCGRG 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 1504
EcoRI GAATTC 1 cut(s) 1378
EcoRII CCWGG 7 cut(s) 1109, 1230, 1271, 1347, 1564, 1723, 2244
EcoRV GATATC 1 cut(s) 2048
EcoT14I CCWWGG 3 cut(s) 275, 292, 608
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 3 cut(s) 1506, 1602, 2118
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 3 cut(s) 275, 292, 608
Esp3I CGTCTC 1 cut(s) 2228
FaeI CATG 8 cut(s) 493, 707, 721, 1103, 1147, 1298, 1538, 1746
FalI AAGNNNNNCTT 2 cut(s) 601, 633
FaqI GGGAC 3 cut(s) 499, 1777, 2322
FatI CATG 8 cut(s) 489, 703, 717, 1099, 1143, 1294, 1534, 1742
FauI CCCGC 2 cut(s) 279, 1739
FauNDI CATATG 3 cut(s) 910, 1797, 2173
FbaI TGATCA 3 cut(s) 823, 1297, 1510
FblI GTMKAC 2 cut(s) 391, 2039
FokI GGATG 8 cut(s) 167, 541, 550, 935, 940, 1271, 1325, 1874
FriOI GRGCYC 3 cut(s) 1506, 1602, 2118
FspBI CTAG 8 cut(s) 395, 660, 995, 1208, 1371, 2157, 2265, 2354
FspI TGCGCA 1 cut(s) 252
GlaI GCGC 4 cut(s) 61, 252, 797, 1916
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 4 cut(s) 259, 818, 2078, 2116
HapII CCGG 7 cut(s) 57, 63, 503, 531, 575, 1602, 2112
HgaI GACGC 2 cut(s) 800, 2210
HhaI GCGC 4 cut(s) 62, 253, 798, 1917
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 8 cut(s) 493, 707, 721, 1103, 1147, 1298, 1538, 1746
Hin6I GCGC 4 cut(s) 60, 251, 796, 1915
HinP1I GCGC 4 cut(s) 60, 251, 796, 1915
HincII GTYRAC 2 cut(s) 1551, 2040
HindII GTYRAC 2 cut(s) 1551, 2040
HindIII AAGCTT 1 cut(s) 1716
HinfI GANTC 9 cut(s) 211, 242, 694, 1088, 1561, 1960, 1999, 2166, 2204
HpaII CCGG 7 cut(s) 57, 63, 503, 531, 575, 1602, 2112
HphI GGTGA 4 cut(s) 77, 543, 1353, 1436
Hpy166II GTNNAC 4 cut(s) 392, 1551, 2040, 2321
Hpy188I TCNGA 9 cut(s) 21, 185, 204, 742, 1120, 1258, 1266, 1302, 1660
Hpy8I GTNNAC 4 cut(s) 392, 1551, 2040, 2321
Hpy99I CGWCG 1 cut(s) 2044
HpyCH4III ACNGT 5 cut(s) 11, 1095, 2093, 2128, 2275
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 149, 259, 860, 1057, 2075
HpyF3I CTNAG 7 cut(s) 195, 1071, 1117, 1215, 1404, 1521, 1659
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 8 cut(s) 493, 707, 721, 1103, 1147, 1298, 1538, 1746
HspAI GCGC 4 cut(s) 60, 251, 796, 1915
KasI GGCGCC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Ksp22I TGATCA 3 cut(s) 823, 1297, 1510
KspI CCGCGG 1 cut(s) 1423
LguI GCTCTTC 1 cut(s) 1632
LmnI GCTCC 5 cut(s) 80, 439, 966, 1065, 1829
Lsp1109I GCAGC 9 cut(s) 123, 130, 483, 526, 838, 1000, 1184, 1397, 1651
LweI GCATC 7 cut(s) 421, 627, 838, 863, 913, 994, 1417
MaeI CTAG 8 cut(s) 395, 660, 995, 1208, 1371, 2157, 2265, 2354
MaeIII GTNAC 5 cut(s) 11, 1089, 1471, 1989, 2093
MbiI CCGCTC 1 cut(s) 286
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 4 cut(s) 679, 838, 1282, 1516
MhlI GDGCHC 4 cut(s) 1506, 1602, 2118, 2283
MlsI TGGCCA 1 cut(s) 2078
MluNI TGGCCA 1 cut(s) 2078
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 3 cut(s) 688, 1097, 1570
MmeI TCCRAC 1 cut(s) 2143
Mox20I TGGCCA 1 cut(s) 2078
Mph1103I ATGCAT 1 cut(s) 916
MroNI GCCGGC 1 cut(s) 530
MroXI GAANNNNTTC 2 cut(s) 1209, 1719
MscI TGGCCA 1 cut(s) 2078
MslI CAYNNNNRTG 3 cut(s) 708, 1142, 1194
Msp20I TGGCCA 1 cut(s) 2078
MspA1I CMGCKG 1 cut(s) 1422
MspI CCGG 7 cut(s) 57, 63, 503, 531, 575, 1602, 2112
MunI CAATTG 1 cut(s) 1014
MvaI CCWGG 7 cut(s) 1111, 1232, 1273, 1349, 1566, 1725, 2246
MvnI CGCG 2 cut(s) 262, 1422
MwoI GCNNNNNNNGC 6 cut(s) 140, 149, 259, 860, 1057, 2075
NaeI GCCGGC 1 cut(s) 532
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 4 cut(s) 504, 576, 1602, 2113
NdeI CATATG 3 cut(s) 910, 1797, 2173
NgoMIV GCCGGC 1 cut(s) 530
NheI GCTAGC 1 cut(s) 1370
NlaIII CATG 8 cut(s) 493, 707, 721, 1103, 1147, 1298, 1538, 1746
NlaIV GGNNCC 8 cut(s) 61, 290, 487, 614, 1338, 1606, 2064, 2116
NmeAIII GCCGAG 1 cut(s) 2011
NmuCI GTSAC 2 cut(s) 1089, 1471
NsbI TGCGCA 1 cut(s) 252
NsiI ATGCAT 1 cut(s) 916
NspI RCATGY 1 cut(s) 1746
PaeI GCATGC 1 cut(s) 1746
PagI TCATGA 1 cut(s) 1294
PasI CCCWGGG 1 cut(s) 1231
PciSI GCTCTTC 1 cut(s) 1632
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 532
PdmI GAANNNNTTC 2 cut(s) 1209, 1719
PfeI GAWTC 6 cut(s) 211, 242, 1960, 1999, 2166, 2204
PflMI CCANNNNNTGG 1 cut(s) 1278
PfoI TCCNGGA 2 cut(s) 574, 1564
Ple19I CGATCG 1 cut(s) 19
PleI GAGTC 3 cut(s) 688, 1096, 1569
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 3 cut(s) 688, 1096, 1569
PshAI GACNNNNGTC 1 cut(s) 736
PshBI ATTAAT 1 cut(s) 1758
Psp124BI GAGCTC 1 cut(s) 1506
Psp6I CCWGG 7 cut(s) 1109, 1230, 1271, 1347, 1564, 1723, 2244
PspGI CCWGG 7 cut(s) 1109, 1230, 1271, 1347, 1564, 1723, 2244
PspN4I GGNNCC 8 cut(s) 61, 290, 487, 614, 1338, 1606, 2064, 2116
PspOMI GGGCCC 1 cut(s) 2114
PspPI GGNCC 4 cut(s) 289, 486, 2114, 2115
PstI CTGCAG 2 cut(s) 1387, 1669
PsuI RGATCY 4 cut(s) 679, 838, 1282, 1516
PvuI CGATCG 1 cut(s) 19
RsaI GTAC 8 cut(s) 980, 1104, 1242, 1751, 1791, 2110, 2125, 2322
RsaNI GTAC 8 cut(s) 979, 1103, 1241, 1750, 1790, 2109, 2124, 2321
RseI CAYNNNNRTG 3 cut(s) 708, 1142, 1194
SacI GAGCTC 1 cut(s) 1506
SacII CCGCGG 1 cut(s) 1423
SalI GTCGAC 1 cut(s) 2038
SapI GCTCTTC 1 cut(s) 1632
Sau96I GGNCC 4 cut(s) 289, 486, 2114, 2115
ScaI AGTACT 1 cut(s) 980
SchI GAGTC 3 cut(s) 688, 1097, 1570
SduI GDGCHC 4 cut(s) 1506, 1602, 2118, 2283
SfaNI GCATC 7 cut(s) 421, 627, 838, 863, 913, 994, 1417
SfcI CTRYAG 3 cut(s) 1383, 1665, 2184
SfoI GGCGCC 1 cut(s) 61
Sfr303I CCGCGG 1 cut(s) 1423
SgrAI CRCCGGYG 1 cut(s) 62
SgrBI CCGCGG 1 cut(s) 1423
SinI GGWCC 2 cut(s) 289, 486
SmiMI CAYNNNNRTG 3 cut(s) 708, 1142, 1194
SmlI CTYRAG 3 cut(s) 215, 466, 788
SmoI CTYRAG 3 cut(s) 215, 466, 788
SpeI ACTAGT 3 cut(s) 394, 1207, 2156
SphI GCATGC 1 cut(s) 1746
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 8 cut(s) 395, 660, 995, 1208, 1371, 2157, 2265, 2354
SstI GAGCTC 1 cut(s) 1506
StyI CCWWGG 3 cut(s) 275, 292, 608
TaaI ACNGT 5 cut(s) 11, 1095, 2093, 2128, 2275
TaqI TCGA 5 cut(s) 857, 941, 1506, 1824, 2039
TatI WGTACW 2 cut(s) 978, 2320
TauI GCSGC 1 cut(s) 262
TfiI GAWTC 6 cut(s) 211, 242, 1960, 1999, 2166, 2204
TscAI CASTG 2 cut(s) 2125, 2140
TseFI GTSAC 2 cut(s) 1089, 1471
TseI GCWGC 9 cut(s) 111, 143, 471, 514, 851, 988, 1172, 1385, 1664
Tsp45I GTSAC 2 cut(s) 1089, 1471
TspDTI ATGAA 6 cut(s) 318, 506, 720, 942, 1641, 2010
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 2 cut(s) 2125, 2140
Van91I CCANNNNNTGG 1 cut(s) 1278
VpaK11BI GGWCC 2 cut(s) 289, 486
VspI ATTAAT 1 cut(s) 1758
XapI RAATTY 3 cut(s) 23, 1378, 1704
XbaI TCTAGA 2 cut(s) 2264, 2353
XceI RCATGY 1 cut(s) 1746
XcmI CCANNNNNNNNNTGG 1 cut(s) 1802
XmiI GTMKAC 2 cut(s) 391, 2039
XmnI GAANNNNTTC 2 cut(s) 1209, 1719
XspI CTAG 8 cut(s) 395, 660, 995, 1208, 1371, 2157, 2265, 2354
ZrmI AGTACT 1 cut(s) 980
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.