pycom03g16520

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001142
Physical Location & Seq
Forward (+)
17696224 .. 17697763
1540 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16520.1

Sequence Viewer

Length: 1500 bp
ATGGAGAACGGTCACGATTGGAAAGTCGCGGAGAAATTCTCAGGGTTGACGGTTTCCGCCGCCGACCCAATTCCTTCTTCTACCCCCACCGCCACCCCAAACAATCAAGACAGCAGCTTGATTCAGGTTATGAACGCCATGGAAGCTGCGGAGGCCACAATCAAGCAGCAGGTGGAAGAGAACATCCGATTGAGGAATCAGCTCGAACACAAGATTCTCGAGTTGCACAGGTACAAGCTCCACGAATCCATGGCTGCCCAGTTGCCGCCTTCTGCCGGCGGATCTCAGGACCAAGCTCATCAGGTAGTTGCCCCCGGCGTCGACAATCGAATCGAGAGGATTGCGACTCACGGTTCTGCACAGCATAGTCGCGGGGAATCGGAAACAAGAGAGACCCAATTGGGCGGCGGCGAAATCAATGGAGGAAAGGTGGTTCGGGTTTCGGGTACTCCGCGGGCGGCTGATAACGCGAGCAGCGGGATATCGAGATTGTCGTCGCCATCCACCACGACGTCGTTTTCGCCAGGTAGGTCGAGCACGGAGGGGGATTACGGAAATGGGTTGATGCCATTGACCGAATCGAACAACCCTAATAGCAGCATGTGGAAGAAGGATCTGGTTGGTAGGGTTCATGAGCACGAGCAAGAAATTACGCAATTGCGAAAGCATTTGGCGGATTATTCAATCAAAGAGGCGGAAATTCGGAACGAAAAGTATGTCGAGCAGCAGCAGGACCTTGTGGATGCTGCATCAAAGTCTCTATCTTACAGGCAAGACATAATTGAGGAGAACATTCGGCTCGCTTACGCCGTGCAGGATGCGCAGCAGGAGAGATCCACGTTTGTTTCGTCTTTGCTGCCGATTCTGGCTGAGTACTCGTTGCAGCCACCCGTCCCCGATGCGCAATCAATTGTTGGTAATGTGAAGGTTTTGTTTAGGCATTTGCAGGAGAAGTTGCTTCTTACTGAGTCCAAATTGAAGGAGTTGCAGTATCAGTTAACGCCTTGGCGACCAGATTCGAATCAGCAGCCTAATAATGTTAATGTTTCTGCACCGCAGTTATCACCACCGCCGCCACCATCACCGCCACCACCTCAGTCCATTGCTGGAGCAGCATTGCTGAATGTAAGCAGAAATGCTCTTGAAATGGTTGCTCAGAATCAGCAAGCATATTCTTACATTGGTGGGAGCAGGGCGCTTGCTACCTCTGATGCTCAGACAACCCCGGAATGGAATCCTCATCAGCAGAGTGGTTTTGGTGGGGTTGTCGCCAACAATGTGGAACACAATGACCTGGTCATGGGGAATAGGTATTCCCCTGATCCAAACCGGATTATTGCTGCCCAAGATGTACCCATTAATCATGCTGTTGTTCCAGGTGATACGCAAGGAAATCGGCATAATGAAGTAACAAGCAATAGACAGGTGACATTTCGTGATCCTATCAGGAACACACACAGAGAGATGGATCGATCAAGATGCAGAGGGAAACCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

500

Amino Acids

54.83

Weight (kDa)

5.99

Isoelectric Point (pI)

51.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
AatII GACGTC 1 cut(s) 515
Acc16I TGCGCA 2 cut(s) 822, 903
Acc36I ACCTGC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 321
AccII CGCG 4 cut(s) 29, 372, 454, 470
AclWI GGATC 6 cut(s) 289, 621, 828, 1316, 1433, 1476
AcsI RAATTY 2 cut(s) 35, 699
AcyI GRCGYC 2 cut(s) 318, 512
AfaI GTAC 4 cut(s) 233, 448, 875, 1353
AfiI CCNNNNNNNGG 3 cut(s) 275, 1230, 1300
AgsI TTSAA 3 cut(s) 684, 979, 1145
AjnI CCWGG 3 cut(s) 523, 1293, 1375
AjuI GAANNNNNNNTTGG 2 cut(s) 61, 93
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 5 cut(s) 117, 146, 202, 238, 296
AluI AGCT 5 cut(s) 117, 146, 202, 238, 296
Alw21I GWGCWC 2 cut(s) 539, 639
Alw26I GTCTC 2 cut(s) 386, 762
AlwI GGATC 6 cut(s) 289, 621, 828, 1316, 1433, 1476
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 1 cut(s) 153
ApoI RAATTY 2 cut(s) 35, 699
ArsI GACNNNNNNTTYG 4 cut(s) 502, 534, 702, 734
AseI ATTAAT 1 cut(s) 1359
AspLEI GCGC 3 cut(s) 823, 904, 1198
AspS9I GGNCC 2 cut(s) 289, 733
AsuC2I CCSGG 2 cut(s) 315, 1226
AsuHPI GGTGA 4 cut(s) 1056, 1074, 1391, 1438
AsuII TTCGAA 1 cut(s) 1019
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 2 cut(s) 289, 733
BauI CACGAG 1 cut(s) 638
Bbv12I GWGCWC 2 cut(s) 539, 639
BccI CCATC 3 cut(s) 508, 1087, 1459
BceAI ACGGC 1 cut(s) 794
BcgI CGANNNNNNTGC 6 cut(s) 323, 357, 1376, 1410, 1461, 1495
BciT130I CCWGG 3 cut(s) 525, 1295, 1377
BcnI CCSGG 2 cut(s) 315, 1226
BcoDI GTCTC 2 cut(s) 386, 762
BfoI RGCGCY 1 cut(s) 1199
BfuAI ACCTGC 1 cut(s) 160
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 5 cut(s) 315, 525, 1226, 1295, 1377
Bme18I GGWCC 2 cut(s) 289, 733
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 2 cut(s) 289, 733
BmrFI CCNGG 5 cut(s) 315, 525, 1226, 1295, 1377
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 7 cut(s) 555, 733, 758, 808, 889, 1201, 1469
BmuI ACTGGG 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 23, 55
BpmI CTGGAG 1 cut(s) 1128
Bpu14I TTCGAA 1 cut(s) 1019
BpuMI CCSGG 2 cut(s) 315, 1226
Bsa29I ATCGAT 1 cut(s) 1471
BsaBI GATNNNNATC 1 cut(s) 1020
BsaHI GRCGYC 2 cut(s) 318, 512
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 6 cut(s) 138, 249, 313, 452, 1004, 1224
BsaWI WCCGGW 1 cut(s) 1329
BsaXI ACNNNNNCTCC 4 cut(s) 414, 444, 974, 1004
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 1230, 1300
Bse118I RCCGGY 1 cut(s) 275
Bse1I ACTGG 1 cut(s) 259
Bse3DI GCAATG 2 cut(s) 1101, 1115
Bse8I GATNNNNATC 1 cut(s) 1020
BseBI CCWGG 3 cut(s) 525, 1295, 1377
BseCI ATCGAT 1 cut(s) 1471
BseDI CCNNGG 6 cut(s) 138, 249, 313, 452, 1004, 1224
BseGI GGATG 4 cut(s) 183, 500, 748, 823
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 3 cut(s) 275, 1230, 1300
BseMI GCAATG 2 cut(s) 1101, 1115
BseMII CTCAG 7 cut(s) 54, 299, 861, 957, 1109, 1169, 1229
BseNI ACTGG 1 cut(s) 259
BseRI GAGGAG 1 cut(s) 800
BsgI GTGCAG 3 cut(s) 342, 833, 1035
Bsh1236I CGCG 4 cut(s) 29, 372, 454, 470
BshFI GGCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 1471
BsiHKAI GWGCWC 2 cut(s) 539, 639
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 4 cut(s) 276, 315, 1226, 1330
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 3 cut(s) 275, 1230, 1300
BsmAI GTCTC 2 cut(s) 386, 762
BsmFI GGGAC 1 cut(s) 878
BsnI GGCC 1 cut(s) 155
Bso31I GGTCTC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 1019
Bsp1286I GDGCHC 2 cut(s) 539, 639
Bsp143I GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
Bsp19I CCATGG 2 cut(s) 138, 249
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 7 cut(s) 53, 298, 862, 958, 1108, 1168, 1228
BspDI ATCGAT 1 cut(s) 1471
BspFNI CGCG 4 cut(s) 29, 372, 454, 470
BspHI TCATGA 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 6 cut(s) 289, 621, 828, 1316, 1433, 1476
BspT104I TTCGAA 1 cut(s) 1019
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 1101, 1115
BsrFI RCCGGY 1 cut(s) 275
BsrI ACTGG 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 275
BssECI CCNNGG 6 cut(s) 138, 249, 313, 452, 1004, 1224
BssMI GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
BssNI GRCGYC 2 cut(s) 318, 512
BssSI CACGAG 1 cut(s) 638
BssT1I CCWWGG 3 cut(s) 138, 249, 1004
Bst2BI CACGAG 1 cut(s) 638
Bst2UI CCWGG 3 cut(s) 525, 1295, 1377
Bst4CI ACNGT 3 cut(s) 11, 52, 353
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 2 cut(s) 318, 512
BstBI TTCGAA 1 cut(s) 1019
BstC8I GCNNGC 6 cut(s) 277, 456, 472, 801, 1167, 1200
BstDEI CTNAG 7 cut(s) 40, 285, 870, 966, 1095, 1155, 1215
BstDSI CCRYGG 3 cut(s) 138, 249, 452
BstF5I GGATG 4 cut(s) 183, 500, 748, 823
BstFNI CGCG 4 cut(s) 29, 372, 454, 470
BstH2I RGCGCY 1 cut(s) 1199
BstHHI GCGC 3 cut(s) 823, 904, 1198
BstKTI GATC 7 cut(s) 284, 616, 836, 1324, 1441, 1471, 1475
BstMAI GTCTC 2 cut(s) 386, 762
BstMBI GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
BstMWI GCNNNNNNNGC 5 cut(s) 143, 152, 467, 820, 1112
BstNI CCWGG 3 cut(s) 525, 1295, 1377
BstNSI RCATGY 1 cut(s) 604
BstSCI CCNGG 5 cut(s) 313, 523, 1224, 1293, 1375
BstUI CGCG 4 cut(s) 29, 372, 454, 470
BstX2I RGATCY 3 cut(s) 281, 613, 833
BstXI CCANNNNNNTGG 1 cut(s) 1279
BstYI RGATCY 3 cut(s) 281, 613, 833
Bsu15I ATCGAT 1 cut(s) 1471
BsuRI GGCC 1 cut(s) 155
BsuTUI ATCGAT 1 cut(s) 1471
BtgI CCRYGG 3 cut(s) 138, 249, 452
BtsCI GGATG 4 cut(s) 183, 500, 748, 823
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 6 cut(s) 277, 456, 472, 801, 1167, 1200
CciI TCATGA 1 cut(s) 631
CfoI GCGC 3 cut(s) 823, 904, 1198
Cfr10I RCCGGY 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 289, 733
Cfr42I CCGCGG 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 1471
CseI GACGC 1 cut(s) 307
CsiI ACCWGGT 1 cut(s) 1293
Csp6I GTAC 4 cut(s) 232, 447, 874, 1352
CviAII CATG 6 cut(s) 139, 250, 601, 632, 1300, 1364
CviQI GTAC 4 cut(s) 232, 447, 874, 1352
DdeI CTNAG 7 cut(s) 40, 285, 870, 966, 1095, 1155, 1215
DpnI GATC 7 cut(s) 283, 615, 835, 1323, 1440, 1470, 1474
DpnII GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EciI GGCGGA 4 cut(s) 46, 294, 689, 710
Eco130I CCWWGG 3 cut(s) 138, 249, 1004
Eco31I GGTCTC 1 cut(s) 386
Eco32I GATATC 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 289, 733
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 733
EcoRII CCWGG 3 cut(s) 523, 1293, 1375
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 3 cut(s) 138, 249, 1004
ErhI CCWWGG 3 cut(s) 138, 249, 1004
FaeI CATG 6 cut(s) 142, 253, 604, 635, 1303, 1367
FaqI GGGAC 1 cut(s) 878
FatI CATG 6 cut(s) 138, 249, 600, 631, 1299, 1363
FauI CCCGC 3 cut(s) 365, 447, 470
FblI GTMKAC 1 cut(s) 321
FokI GGATG 4 cut(s) 170, 487, 755, 830
FspI TGCGCA 2 cut(s) 822, 903
GlaI GCGC 3 cut(s) 822, 903, 1197
GsuI CTGGAG 1 cut(s) 1128
HaeII RGCGCY 1 cut(s) 1199
HaeIII GGCC 1 cut(s) 155
HapII CCGG 4 cut(s) 276, 315, 1226, 1330
HgaI GACGC 1 cut(s) 307
HhaI GCGC 3 cut(s) 823, 904, 1198
Hin1I GRCGYC 2 cut(s) 318, 512
Hin1II CATG 6 cut(s) 142, 253, 604, 635, 1303, 1367
Hin6I GCGC 3 cut(s) 821, 902, 1196
HinP1I GCGC 3 cut(s) 821, 902, 1196
HincII GTYRAC 3 cut(s) 48, 322, 999
HindII GTYRAC 3 cut(s) 48, 322, 999
HpaI GTTAAC 1 cut(s) 999
HpaII CCGG 4 cut(s) 276, 315, 1226, 1330
HphI GGTGA 4 cut(s) 1056, 1074, 1391, 1438
Hpy166II GTNNAC 3 cut(s) 48, 322, 999
Hpy188I TCNGA 6 cut(s) 188, 382, 705, 1158, 1210, 1218
Hpy8I GTNNAC 3 cut(s) 48, 322, 999
Hpy99I CGWCG 4 cut(s) 323, 499, 514, 517
HpyAV CCTTC 5 cut(s) 84, 279, 604, 919, 973
HpyCH4III ACNGT 3 cut(s) 11, 52, 353
HpyCH4IV ACGT 2 cut(s) 512, 839
HpyCH4V TGCA 9 cut(s) 226, 359, 749, 814, 883, 946, 988, 1052, 1482
HpyF10VI GCNNNNNNNGC 5 cut(s) 143, 152, 467, 820, 1112
HpyF3I CTNAG 7 cut(s) 40, 285, 870, 966, 1095, 1155, 1215
HpySE526I ACGT 2 cut(s) 512, 839
Hsp92I GRCGYC 2 cut(s) 318, 512
Hsp92II CATG 6 cut(s) 142, 253, 604, 635, 1303, 1367
HspAI GCGC 3 cut(s) 821, 902, 1196
KroI GCCGGC 1 cut(s) 275
KroNI GCCGGC 1 cut(s) 277
KspAI GTTAAC 1 cut(s) 999
KspI CCGCGG 1 cut(s) 455
Kzo9I GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
LmnI GCTCC 3 cut(s) 243, 1109, 1188
LweI GCATC 7 cut(s) 555, 733, 758, 808, 889, 1201, 1469
MabI ACCWGGT 1 cut(s) 1293
MaeII ACGT 2 cut(s) 512, 839
MaeIII GTNAC 3 cut(s) 11, 1408, 1426
MalI GATC 7 cut(s) 283, 615, 835, 1323, 1440, 1470, 1474
MboI GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
MboII GAAGA 3 cut(s) 69, 188, 619
MfeI CAATTG 3 cut(s) 398, 656, 909
MflI RGATCY 3 cut(s) 281, 613, 833
MhlI GDGCHC 2 cut(s) 539, 639
MluCI AATT 9 cut(s) 35, 69, 398, 648, 656, 699, 780, 909, 974
MlyI GAGTC 2 cut(s) 340, 977
MroNI GCCGGC 1 cut(s) 275
MseI TTAA 3 cut(s) 998, 1041, 1359
MspA1I CMGCKG 2 cut(s) 454, 477
MspI CCGG 4 cut(s) 276, 315, 1226, 1330
MspR9I CCNGG 5 cut(s) 315, 525, 1226, 1295, 1377
MunI CAATTG 3 cut(s) 398, 656, 909
MvaI CCWGG 3 cut(s) 525, 1295, 1377
MvnI CGCG 4 cut(s) 29, 372, 454, 470
MwoI GCNNNNNNNGC 5 cut(s) 143, 152, 467, 820, 1112
NaeI GCCGGC 1 cut(s) 277
NciI CCSGG 2 cut(s) 315, 1226
NcoI CCATGG 2 cut(s) 138, 249
NdeII GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
NgoMIV GCCGGC 1 cut(s) 275
NlaIII CATG 6 cut(s) 142, 253, 604, 635, 1303, 1367
NmuCI GTSAC 2 cut(s) 11, 1426
NsbI TGCGCA 2 cut(s) 822, 903
NspI RCATGY 1 cut(s) 604
NspV TTCGAA 1 cut(s) 1019
PaeR7I CTCGAG 1 cut(s) 218
PagI TCATGA 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 3 cut(s) 491, 807, 845
PdiI GCCGGC 1 cut(s) 277
PflFI GACNNNGTC 1 cut(s) 1295
PleI GAGTC 2 cut(s) 340, 976
PpsI GAGTC 2 cut(s) 340, 976
PpuMI RGGWCCY 1 cut(s) 733
PshBI ATTAAT 1 cut(s) 1359
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 3 cut(s) 523, 1293, 1375
PspGI CCWGG 3 cut(s) 523, 1293, 1375
PspPI GGNCC 2 cut(s) 289, 733
PspPPI RGGWCCY 1 cut(s) 733
PsuI RGATCY 3 cut(s) 281, 613, 833
PsyI GACNNNGTC 1 cut(s) 1295
RsaI GTAC 4 cut(s) 233, 448, 875, 1353
RsaNI GTAC 4 cut(s) 232, 447, 874, 1352
SacII CCGCGG 1 cut(s) 455
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 3 cut(s) 998, 1041, 1359
Sau3AI GATC 7 cut(s) 281, 613, 833, 1321, 1438, 1468, 1472
Sau96I GGNCC 2 cut(s) 289, 733
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 2 cut(s) 340, 977
ScrFI CCNGG 5 cut(s) 315, 525, 1226, 1295, 1377
SduI GDGCHC 2 cut(s) 539, 639
SexAI ACCWGGT 1 cut(s) 1293
SfaNI GCATC 7 cut(s) 555, 733, 758, 808, 889, 1201, 1469
Sfr274I CTCGAG 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 455
SfuI TTCGAA 1 cut(s) 1019
SgrBI CCGCGG 1 cut(s) 455
SinI GGWCC 2 cut(s) 289, 733
SlaI CTCGAG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 9 cut(s) 35, 69, 398, 648, 656, 699, 780, 909, 974
StyD4I CCNGG 5 cut(s) 313, 523, 1224, 1293, 1375
StyI CCWWGG 3 cut(s) 138, 249, 1004
TaaI ACNGT 3 cut(s) 11, 52, 353
TaiI ACGT 2 cut(s) 515, 842
TaqII GACCGA 1 cut(s) 590
TasI AATT 9 cut(s) 35, 69, 398, 648, 656, 699, 780, 909, 974
TatI WGTACW 1 cut(s) 873
TauI GCSGC 6 cut(s) 62, 268, 408, 411, 461, 1075
Tru1I TTAA 3 cut(s) 998, 1041, 1359
Tru9I TTAA 3 cut(s) 998, 1041, 1359
TseFI GTSAC 2 cut(s) 11, 1426
Tsp45I GTSAC 2 cut(s) 11, 1426
TspDTI ATGAA 3 cut(s) 146, 620, 1419
TspGWI ACGGA 2 cut(s) 554, 567
Tth111I GACNNNGTC 1 cut(s) 1295
VpaK11BI GGWCC 2 cut(s) 289, 733
VspI ATTAAT 1 cut(s) 1359
XapI RAATTY 2 cut(s) 35, 699
XceI RCATGY 1 cut(s) 604
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 321
ZraI GACGTC 1 cut(s) 513
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.