Prupe.4G196300_v2.0.a1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001142
Physical Location & Seq
Reverse (-)
11970450 .. 11973233
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G196300.1

Sequence Viewer

Length: 2388 bp
ATGGAGAACGGTCACGATTGGAAAATTGCTGAGAAATTCCCAGGTTTGACGATTGCAGGCGCTGACCCAATTCGTTCTTCAAACAACAGCACCAGCAATCAAGACGGAAACTTGCTTCAGGTCATGAAAGCCATGGAAGCTGCAGAGGCCACAATCAAGCAACAGGTTGAAGAGAACATCCGATTGAGGACCCAGCTCGAACACAAGATGCTCGAGCTCCACAGATACAAGCTCCAGGAATCCATGCCCACCCAAATGCCGCCTTCTGTTGATCCATGGGCCCAGCGCCTCCATGGATCTCATGAACAAGCTCATCAACCAGTTGCCCATGTCGACAATCAAATCGATAGGATTGCAAATCCCGCGCAGATTCATAGAGAATCAGAAACCCGAGAAACCCAATTGGGTAGCAGCGAGATCAATGGAGCCATGAAGGTCGTTCGAGTTTCTGGTAATCAGGCAGCTGCAGAAAACGCCAGCAGTGGCATCTCTCGGTTGTCTTCACCATCCACAACGACGTCGCTTTCTCCGGGAAGGTCGAGCACCGAAGGGGAATATGATCCTCAGCGTGTCAATTTTTCTGGACATGGGTTGATGCCATTGACTGAAAGAAACAACCCCAGCAACAGCCTGTGGAAGAAGGATCTGGTTGTTAAGGTTCATGAACATGAACAAGAGATTATGCAATTGCGAAAGCATTTGGCGGATTATTCGATCAAAGAAGCGCAAATTCGAAATGAAAAGTATGTGCTGGAGAAGCGCATTGCTTATATGCGTTTGGCATTTGATCAGCAGCAGCAGGACCTTGCTGATGCTGCATCAAAGGCTCTGTCTTACAGACAGGACATTATTGAGGAAAACATTCGGCTCGCTTATGCCGTGCAGGATGCACAGCAGGAGAGATCGACGTTTGTTTCCTCTTTGCTGCCAATTCTGGCTGAGTACTCATTGCAGCCTCCGGTTCCTGATGCACAGTCCATTGTTGGCAATGTCAAGGTTCTGTTTAGGCATTTGCAGGAGAAGTTGCTTCTTACTGAGTCCAAATTGAAGGAGTTGCAGTATCAGTTAACGCCTTGGCGATCAGATTCGAATCAGCAGCCTAATAACATTAGCATTGCTGCGCCTCCTTCACCACCATCTCAGTCCATTGGTGGTGGAGCATTGCTAAATGTGAGCAGAAATGGGCTTGAAATGGTTCCTCAGAATCAGCCAACATATGCTTCCAATGGTGGAAGAGTGCCAGCTTCGGCTTCTGATACTCAGACAACCGCGGATTGGGATCATCATCCTCAGAGTGGTTTCGGTGGTGGTGCCCCCAACAATGTGGAAACCAATGACTTGGGATTGGGGAGGTATTCACCTGTTCCAAGCAGGACTTCAGCAGGCCAAGATCAAGATGTTCCTGTTAATCTTGCTGTTACTCAAGGTGATACACAGCGGGTAAATCAGTATAGTGGAGGGACAAGCAATAGACAGGTCACCTTTCGTGATCCGGTTGGGAACACACACAGAGAGATGGATGATCAATATGCAGAGGGGAACCAGAATGACAGAGAAACCTCATCCAATTGGAGTGCAGGGAACTCTCCTTACACATCCCCGCTTAACGATTCCAGCTCCCCGTACCCTCATTATCTACCGCCGGTTCTCGAAGAGGGTGGTTCATCTTCATATTCAGAGGCTGCAGATGATGATCCATTACCAGCTATAGAGGGCCTCCAAATTTCGGGTGAAGCTTTTCCGGGGCATGAACTTCAAGCGTGCGGGTACTCTATCAACGGAACAACCAGTTGCAACTTTGAGTGGGTGCGCCACATGCAAGATGGATCTGTCAATTACATTGATGGAGCAAAGCAACCAAATTATCTTGTAACTGCTGATGATGTCGATACATACCTTGCTATCGAAGTCCAGCCTCTGGATAACAGGAAGCGCAAGGGAGAGCTTGTAAAGGTGTTTGCCAATGAGCACAAGAAGATCACTTGTGATACTGAAATGCAAGGCCAGATAGAGAAGATTCTTTATAAGGGTCAGGCTTCATTTAAAATATTTCAGTTCGCTGGATATCTTGACATTTGGGAACCTGCTACATTGGCCATTAAGAGGGGAGCCTACAGCATTAAAGGCAGTGGAGGAACTAGTGGAAGTGGTGTTGTAGTTACAGAGAAGTTTTCGCCGAACACATTTGTCACAATTCCTTATGGAAGTCCTGCAGAATTCATAATAGTTAGCGGCTCGGATGGTGGTGGTGGTGGTGGTGAGTATCTCTTAAGAGCAGACAATGAAAGCTGTTCTAGAGATACAGTTGTGCTCACATTAAGATTATTTATCCATAGGGCTGATGTGAGAAAAAACTCGAGGAGAGGGAAAAAGATGTTATTTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

796

Amino Acids

87.55

Weight (kDa)

5.57

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2025
AatII GACGTC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 2092
AccB1I GGYRCC 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 1918
AccI GTMKAC 1 cut(s) 333
AccII CGCG 2 cut(s) 365, 1271
AclWI GGATC 8 cut(s) 266, 304, 554, 651, 1287, 1484, 1688, 1834
AcoI YGGCCR 1 cut(s) 2094
AcsI RAATTY 4 cut(s) 35, 729, 1722, 2216
AcuI CTGAAG 2 cut(s) 101, 1362
AcyI GRCGYC 1 cut(s) 518
AfaI GTAC 3 cut(s) 944, 1625, 1769
AfiI CCNNNNNNNGG 5 cut(s) 1275, 1295, 1726, 1918, 2103
AflII CTTAAG 1 cut(s) 2269
AgsI TTSAA 5 cut(s) 81, 170, 1048, 1190, 1757
AhlI ACTAGT 1 cut(s) 2138
AjnI CCWGG 2 cut(s) 40, 234
AjuI GAANNNNNNNTTGG 6 cut(s) 61, 93, 1204, 1236, 1360, 1392
Alw21I GWGCWC 4 cut(s) 219, 545, 1971, 2313
AlwI GGATC 8 cut(s) 266, 304, 554, 651, 1287, 1484, 1688, 1834
AlwNI CAGNNNCTG 3 cut(s) 62, 1682, 1918
Ama87I CYCGRG 3 cut(s) 212, 390, 2356
AoxI GGCC 6 cut(s) 147, 279, 1384, 1714, 2002, 2094
ApaI GGGCCC 1 cut(s) 283
ApoI RAATTY 4 cut(s) 35, 729, 1722, 2216
ArsI GACNNNNNNTTYG 2 cut(s) 815, 847
Asp700I GAANNNNTTC 3 cut(s) 861, 1194, 1737
AspLEI GCGC 8 cut(s) 62, 288, 367, 727, 762, 1123, 1812, 1935
AspS9I GGNCC 5 cut(s) 189, 279, 280, 802, 1714
AsuC2I CCSGG 2 cut(s) 531, 1743
AsuHPI GGTGA 7 cut(s) 495, 1122, 1350, 1439, 1471, 1742, 2270
AsuII TTCGAA 2 cut(s) 733, 1088
AvaI CYCGRG 3 cut(s) 212, 390, 2356
AvaII GGWCC 2 cut(s) 189, 802
BaeGI GKGCMC 2 cut(s) 283, 1315
BaeI ACNNNNGTAYC 2 cut(s) 2292, 2325
BalI TGGCCA 1 cut(s) 2096
BanI GGYRCC 1 cut(s) 1310
BanII GRGCYC 2 cut(s) 219, 283
BbsI GAAGAC 1 cut(s) 492
Bbv12I GWGCWC 4 cut(s) 219, 545, 1971, 2313
BbvCI CCTCAGC 1 cut(s) 564
BccI CCATC 6 cut(s) 514, 1144, 1510, 1817, 1838, 2234
BceAI ACGGC 1 cut(s) 863
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 2 cut(s) 42, 236
BclI TGATCA 2 cut(s) 787, 1522
BcnI CCSGG 2 cut(s) 531, 1743
BcuI ACTAGT 1 cut(s) 2138
BfaI CTAG 2 cut(s) 2139, 2295
BfmI CTRYAG 6 cut(s) 141, 465, 1683, 1707, 2113, 2211
BfoI RGCGCY 2 cut(s) 63, 289
BfrI CTTAAG 1 cut(s) 2269
BfuAI ACCTGC 1 cut(s) 2092
BmcAI AGTACT 1 cut(s) 944
Bme1390I CCNGG 4 cut(s) 42, 236, 531, 1743
Bme18I GGWCC 2 cut(s) 189, 802
BmeT110I CYCGRG 3 cut(s) 212, 390, 2356
BmgT120I GGNCC 5 cut(s) 189, 279, 280, 802, 1714
BmiI GGNNCC 9 cut(s) 191, 281, 427, 963, 1197, 1312, 1541, 2082, 2110
BmrFI CCNGG 4 cut(s) 42, 236, 531, 1743
BmsI GCATC 7 cut(s) 198, 495, 585, 802, 827, 877, 958
BpiI GAAGAC 1 cut(s) 492
BpmI CTGGAG 2 cut(s) 218, 773
Bpu10I CCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 2 cut(s) 733, 1088
BpuEI CTTGAG 1 cut(s) 1407
BpuMI CCSGG 2 cut(s) 531, 1743
Bsa29I ATCGAT 1 cut(s) 345
BsaBI GATNNNNATC 4 cut(s) 1089, 1278, 1284, 1692
BsaHI GRCGYC 1 cut(s) 518
BsaJI CCNNGG 7 cut(s) 40, 132, 275, 292, 1073, 1269, 1742
BsaWI WCCGGW 2 cut(s) 958, 1492
BsaXI ACNNNNNCTCC 4 cut(s) 1043, 1073, 1932, 1962
Bsc4I CCNNNNNNNGG 5 cut(s) 1275, 1295, 1726, 1918, 2103
Bse118I RCCGGY 1 cut(s) 1642
Bse1I ACTGG 2 cut(s) 320, 1788
Bse3DI GCAATG 5 cut(s) 762, 947, 994, 1113, 1160
Bse8I GATNNNNATC 4 cut(s) 1089, 1278, 1284, 1692
BseBI CCWGG 2 cut(s) 42, 236
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 7 cut(s) 40, 132, 275, 292, 1073, 1269, 1742
BseGI GGATG 8 cut(s) 177, 506, 892, 1285, 1525, 1562, 1595, 2245
BseJI GATNNNNATC 4 cut(s) 1089, 1278, 1284, 1692
BseLI CCNNNNNNNGG 5 cut(s) 1275, 1295, 1726, 1918, 2103
BseMI GCAATG 5 cut(s) 762, 947, 994, 1113, 1160
BseMII CTCAG 8 cut(s) 21, 578, 930, 1026, 1154, 1214, 1274, 1304
BseNI ACTGG 2 cut(s) 320, 1788
BseRI GAGGAG 1 cut(s) 2374
BseSI GKGCMC 2 cut(s) 283, 1315
BseYI CCCAGC 3 cut(s) 192, 282, 620
BsgI GTGCAG 2 cut(s) 902, 1596
Bsh1236I CGCG 2 cut(s) 365, 1271
BshFI GGCC 6 cut(s) 149, 281, 1386, 1716, 2004, 2096
BshNI GGYRCC 1 cut(s) 1310
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 4 cut(s) 219, 545, 1971, 2313
BsiHKCI CYCGRG 3 cut(s) 212, 390, 2356
BsiSI CCGG 5 cut(s) 530, 959, 1493, 1643, 1742
BslFI GGGAC 1 cut(s) 1474
BslI CCNNNNNNNGG 5 cut(s) 1275, 1295, 1726, 1918, 2103
BsmFI GGGAC 1 cut(s) 1474
BsnI GGCC 6 cut(s) 149, 281, 1386, 1716, 2004, 2096
BsoBI CYCGRG 3 cut(s) 212, 390, 2356
Bsp119I TTCGAA 2 cut(s) 733, 1088
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 6 cut(s) 219, 283, 545, 1315, 1971, 2313
Bsp19I CCATGG 3 cut(s) 132, 275, 292
BspANI GGCC 6 cut(s) 149, 281, 1386, 1716, 2004, 2096
BspCNI CTCAG 8 cut(s) 22, 577, 931, 1027, 1153, 1213, 1273, 1303
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 2 cut(s) 365, 1271
BspHI TCATGA 3 cut(s) 123, 301, 661
BspLI GGNNCC 9 cut(s) 191, 281, 427, 963, 1197, 1312, 1541, 2082, 2110
BspMAI CTGCAG 4 cut(s) 145, 469, 1687, 2215
BspMI ACCTGC 1 cut(s) 2092
BspPI GGATC 8 cut(s) 266, 304, 554, 651, 1287, 1484, 1688, 1834
BspT104I TTCGAA 2 cut(s) 733, 1088
BspT107I GGYRCC 1 cut(s) 1310
BspTI CTTAAG 1 cut(s) 2269
BsrDI GCAATG 5 cut(s) 762, 947, 994, 1113, 1160
BsrFI RCCGGY 1 cut(s) 1642
BsrI ACTGG 2 cut(s) 320, 1788
BssAI RCCGGY 1 cut(s) 1642
BssECI CCNNGG 7 cut(s) 40, 132, 275, 292, 1073, 1269, 1742
BssNI GRCGYC 1 cut(s) 518
BssT1I CCWWGG 4 cut(s) 132, 275, 292, 1073
Bst2UI CCWGG 2 cut(s) 42, 236
Bst4CI ACNGT 3 cut(s) 11, 975, 2305
Bst6I CTCTTC 3 cut(s) 165, 1228, 1647
BstACI GRCGYC 1 cut(s) 518
BstAFI CTTAAG 1 cut(s) 2269
BstBI TTCGAA 2 cut(s) 733, 1088
BstC8I GCNNGC 6 cut(s) 58, 478, 870, 1242, 1384, 1762
BstDEI CTNAG 8 cut(s) 30, 564, 939, 1035, 1140, 1200, 1260, 1290
BstDSI CCRYGG 4 cut(s) 132, 275, 292, 1269
BstEII GGTNACC 1 cut(s) 1477
BstF5I GGATG 8 cut(s) 177, 506, 892, 1285, 1525, 1562, 1595, 2245
BstFNI CGCG 2 cut(s) 365, 1271
BstH2I RGCGCY 2 cut(s) 63, 289
BstHHI GCGC 8 cut(s) 62, 288, 367, 727, 762, 1123, 1812, 1935
BstNI CCWGG 2 cut(s) 42, 236
BstNSI RCATGY 1 cut(s) 1819
BstPI GGTNACC 1 cut(s) 1477
BstSCI CCNGG 4 cut(s) 40, 234, 529, 1741
BstSFI CTRYAG 6 cut(s) 141, 465, 1683, 1707, 2113, 2211
BstSLI GKGCMC 2 cut(s) 283, 1315
BstUI CGCG 2 cut(s) 365, 1271
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 3 cut(s) 296, 643, 1826
BstXI CCANNNNNNTGG 2 cut(s) 1324, 1339
BstYI RGATCY 3 cut(s) 296, 643, 1826
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 6 cut(s) 149, 281, 1386, 1716, 2004, 2096
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 4 cut(s) 132, 275, 292, 1269
BtsCI GGATG 8 cut(s) 177, 506, 892, 1285, 1525, 1562, 1595, 2245
BtsI GCAGTG 2 cut(s) 487, 2134
BtsIMutI CAGTG 2 cut(s) 487, 2134
BveI ACCTGC 1 cut(s) 2092
Cac8I GCNNGC 6 cut(s) 58, 478, 870, 1242, 1384, 1762
CaiI CAGNNNCTG 3 cut(s) 62, 1682, 1918
CciI TCATGA 3 cut(s) 123, 301, 661
CfoI GCGC 8 cut(s) 62, 288, 367, 727, 762, 1123, 1812, 1935
Cfr10I RCCGGY 1 cut(s) 1642
Cfr13I GGNCC 5 cut(s) 189, 279, 280, 802, 1714
Cfr42I CCGCGG 1 cut(s) 1272
ClaI ATCGAT 1 cut(s) 345
Csp6I GTAC 3 cut(s) 943, 1624, 1768
CviQI GTAC 3 cut(s) 943, 1624, 1768
DdeI CTNAG 8 cut(s) 30, 564, 939, 1035, 1140, 1200, 1260, 1290
DraI TTTAAA 1 cut(s) 2044
EaeI YGGCCR 1 cut(s) 2094
Eam1104I CTCTTC 3 cut(s) 165, 1228, 1647
EarI CTCTTC 3 cut(s) 165, 1228, 1647
EciI GGCGGA 1 cut(s) 719
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 4 cut(s) 132, 275, 292, 1073
Eco24I GRGCYC 2 cut(s) 219, 283
Eco32I GATATC 1 cut(s) 2066
Eco47I GGWCC 2 cut(s) 189, 802
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 101, 1362
Eco88I CYCGRG 3 cut(s) 212, 390, 2356
Eco91I GGTNACC 1 cut(s) 1477
EcoICRI GAGCTC 1 cut(s) 217
EcoO109I RGGNCCY 3 cut(s) 189, 802, 1714
EcoO65I GGTNACC 1 cut(s) 1477
EcoRI GAATTC 1 cut(s) 2216
EcoRII CCWGG 2 cut(s) 40, 234
EcoRV GATATC 1 cut(s) 2066
EcoT14I CCWWGG 4 cut(s) 132, 275, 292, 1073
EcoT38I GRGCYC 2 cut(s) 219, 283
ErhI CCWWGG 4 cut(s) 132, 275, 292, 1073
FalI AAGNNNNNCTT 2 cut(s) 1360, 1392
FaqI GGGAC 1 cut(s) 1474
FauI CCCGC 4 cut(s) 370, 1431, 1608, 1757
FauNDI CATATG 1 cut(s) 1216
FbaI TGATCA 2 cut(s) 787, 1522
FblI GTMKAC 1 cut(s) 333
FokI GGATG 8 cut(s) 164, 493, 899, 1272, 1532, 1549, 1582, 2252
FriOI GRGCYC 2 cut(s) 219, 283
FspBI CTAG 2 cut(s) 2139, 2295
GlaI GCGC 8 cut(s) 61, 287, 366, 726, 761, 1122, 1811, 1934
GsaI CCCAGC 3 cut(s) 196, 286, 624
GsuI CTGGAG 2 cut(s) 218, 773
HaeII RGCGCY 2 cut(s) 63, 289
HaeIII GGCC 6 cut(s) 149, 281, 1386, 1716, 2004, 2096
HapII CCGG 5 cut(s) 530, 959, 1493, 1643, 1742
HhaI GCGC 8 cut(s) 62, 288, 367, 727, 762, 1123, 1812, 1935
Hin1I GRCGYC 1 cut(s) 518
Hin6I GCGC 8 cut(s) 60, 286, 365, 725, 760, 1121, 1810, 1933
HinP1I GCGC 8 cut(s) 60, 286, 365, 725, 760, 1121, 1810, 1933
HincII GTYRAC 2 cut(s) 334, 1068
HindII GTYRAC 2 cut(s) 334, 1068
HindIII AAGCTT 1 cut(s) 1734
HinfI GANTC 9 cut(s) 239, 370, 380, 1037, 1085, 1090, 1204, 1610, 2017
HpaI GTTAAC 1 cut(s) 1068
HpaII CCGG 5 cut(s) 530, 959, 1493, 1643, 1742
HphI GGTGA 7 cut(s) 495, 1122, 1350, 1439, 1471, 1742, 2270
Hpy166II GTNNAC 2 cut(s) 334, 1068
Hpy188I TCNGA 9 cut(s) 182, 385, 1084, 1203, 1255, 1263, 1293, 1678, 2239
Hpy8I GTNNAC 2 cut(s) 334, 1068
Hpy99I CGWCG 3 cut(s) 520, 523, 910
HpyAV CCTTC 7 cut(s) 273, 427, 528, 542, 634, 1042, 1137
HpyCH4III ACNGT 3 cut(s) 11, 975, 2305
HpyCH4IV ACGT 2 cut(s) 518, 908
HpyF3I CTNAG 8 cut(s) 30, 564, 939, 1035, 1140, 1200, 1260, 1290
HpySE526I ACGT 2 cut(s) 518, 908
Hsp92I GRCGYC 1 cut(s) 518
HspAI GCGC 8 cut(s) 60, 286, 365, 725, 760, 1121, 1810, 1933
Ksp22I TGATCA 2 cut(s) 787, 1522
KspAI GTTAAC 1 cut(s) 1068
KspI CCGCGG 1 cut(s) 1272
LmnI GCTCC 7 cut(s) 222, 237, 425, 1157, 1622, 1847, 2108
LweI GCATC 7 cut(s) 198, 495, 585, 802, 827, 877, 958
MaeI CTAG 2 cut(s) 2139, 2295
MaeII ACGT 2 cut(s) 518, 908
MaeIII GTNAC 6 cut(s) 11, 1417, 1477, 1870, 2158, 2188
MboII GAAGA 9 cut(s) 69, 182, 492, 649, 1245, 1659, 1664, 1987, 2026
MfeI CAATTG 3 cut(s) 401, 686, 1567
MflI RGATCY 3 cut(s) 296, 643, 1826
MhlI GDGCHC 6 cut(s) 219, 283, 545, 1315, 1971, 2313
MlsI TGGCCA 1 cut(s) 2096
MluNI TGGCCA 1 cut(s) 2096
MlyI GAGTC 1 cut(s) 1046
Mox20I TGGCCA 1 cut(s) 2096
MroXI GAANNNNTTC 3 cut(s) 861, 1194, 1737
MscI TGGCCA 1 cut(s) 2096
MslI CAYNNNNRTG 2 cut(s) 254, 666
Msp20I TGGCCA 1 cut(s) 2096
MspA1I CMGCKG 3 cut(s) 464, 1271, 1438
MspCI CTTAAG 1 cut(s) 2269
MspI CCGG 5 cut(s) 530, 959, 1493, 1643, 1742
MspR9I CCNGG 4 cut(s) 42, 236, 531, 1743
MunI CAATTG 3 cut(s) 401, 686, 1567
MvaI CCWGG 2 cut(s) 42, 236
MvnI CGCG 2 cut(s) 365, 1271
NciI CCSGG 2 cut(s) 531, 1743
NcoI CCATGG 3 cut(s) 132, 275, 292
NdeI CATATG 1 cut(s) 1216
NlaIV GGNNCC 9 cut(s) 191, 281, 427, 963, 1197, 1312, 1541, 2082, 2110
NmuCI GTSAC 3 cut(s) 11, 1477, 2188
NspI RCATGY 1 cut(s) 1819
NspV TTCGAA 2 cut(s) 733, 1088
PaeR7I CTCGAG 2 cut(s) 212, 2356
PagI TCATGA 3 cut(s) 123, 301, 661
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PdmI GAANNNNTTC 3 cut(s) 861, 1194, 1737
PfeI GAWTC 8 cut(s) 239, 370, 380, 1085, 1090, 1204, 1610, 2017
PflMI CCANNNNNTGG 1 cut(s) 1918
PfoI TCCNGGA 2 cut(s) 234, 529
PleI GAGTC 1 cut(s) 1045
PpsI GAGTC 1 cut(s) 1045
PpuMI RGGWCCY 2 cut(s) 189, 802
PsiI TTATAA 1 cut(s) 2025
Psp124BI GAGCTC 1 cut(s) 219
Psp5II RGGWCCY 2 cut(s) 189, 802
Psp6I CCWGG 2 cut(s) 40, 234
PspEI GGTNACC 1 cut(s) 1477
PspFI CCCAGC 3 cut(s) 192, 282, 620
PspGI CCWGG 2 cut(s) 40, 234
PspN4I GGNNCC 9 cut(s) 191, 281, 427, 963, 1197, 1312, 1541, 2082, 2110
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 5 cut(s) 189, 279, 280, 802, 1714
PspPPI RGGWCCY 2 cut(s) 189, 802
PspXI VCTCGAGB 2 cut(s) 212, 2356
PsrI GAACNNNNNNTAC 2 cut(s) 1574, 1606
PstI CTGCAG 4 cut(s) 145, 469, 1687, 2215
PstNI CAGNNNCTG 3 cut(s) 62, 1682, 1918
PsuI RGATCY 3 cut(s) 296, 643, 1826
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 3 cut(s) 944, 1625, 1769
RsaNI GTAC 3 cut(s) 943, 1624, 1768
RseI CAYNNNNRTG 2 cut(s) 254, 666
SacI GAGCTC 1 cut(s) 219
SacII CCGCGG 1 cut(s) 1272
SalI GTCGAC 1 cut(s) 332
Sau96I GGNCC 5 cut(s) 189, 279, 280, 802, 1714
ScaI AGTACT 1 cut(s) 944
SchI GAGTC 1 cut(s) 1046
ScrFI CCNGG 4 cut(s) 42, 236, 531, 1743
SduI GDGCHC 6 cut(s) 219, 283, 545, 1315, 1971, 2313
SfaNI GCATC 7 cut(s) 198, 495, 585, 802, 827, 877, 958
SfcI CTRYAG 6 cut(s) 141, 465, 1683, 1707, 2113, 2211
Sfr274I CTCGAG 2 cut(s) 212, 2356
Sfr303I CCGCGG 1 cut(s) 1272
SfuI TTCGAA 2 cut(s) 733, 1088
SgrBI CCGCGG 1 cut(s) 1272
SinI GGWCC 2 cut(s) 189, 802
SlaI CTCGAG 2 cut(s) 212, 2356
SmiMI CAYNNNNRTG 2 cut(s) 254, 666
SmlI CTYRAG 4 cut(s) 212, 1422, 2269, 2356
SmoI CTYRAG 4 cut(s) 212, 1422, 2269, 2356
SpeI ACTAGT 1 cut(s) 2138
SspI AATATT 1 cut(s) 2049
SspMI CTAG 2 cut(s) 2139, 2295
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 4 cut(s) 40, 234, 529, 1741
StyI CCWWGG 4 cut(s) 132, 275, 292, 1073
TaaI ACNGT 3 cut(s) 11, 975, 2305
TaiI ACGT 2 cut(s) 521, 911
TatI WGTACW 1 cut(s) 942
TauI GCSGC 2 cut(s) 262, 2235
TfiI GAWTC 8 cut(s) 239, 370, 380, 1085, 1090, 1204, 1610, 2017
TscAI CASTG 2 cut(s) 487, 2134
TseFI GTSAC 3 cut(s) 11, 1477, 2188
Tsp45I GTSAC 3 cut(s) 11, 1477, 2188
TspGWI ACGGA 2 cut(s) 120, 1794
TspRI CASTG 2 cut(s) 487, 2134
Van91I CCANNNNNTGG 1 cut(s) 1918
Vha464I CTTAAG 1 cut(s) 2269
VpaK11BI GGWCC 2 cut(s) 189, 802
XapI RAATTY 4 cut(s) 35, 729, 1722, 2216
XbaI TCTAGA 1 cut(s) 2294
XceI RCATGY 1 cut(s) 1819
XcmI CCANNNNNNNNNTGG 2 cut(s) 290, 1820
XhoI CTCGAG 2 cut(s) 212, 2356
XmiI GTMKAC 1 cut(s) 333
XmnI GAANNNNTTC 3 cut(s) 861, 1194, 1737
XspI CTAG 2 cut(s) 2139, 2295
ZraI GACGTC 1 cut(s) 519
ZrmI AGTACT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.