isoform X1
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 2104 |
| AccB1I | GGYRCC | 1 cut(s) | 62 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1305 |
| AccII | CGCG | 2 cut(s) | 268, 294 |
| AciI | CCGC | 7 cut(s) | 60, 152, 266, 386, 479, 548, 1776 |
| AcoI | YGGCCR | 1 cut(s) | 2106 |
| AcsI | RAATTY | 5 cut(s) | 23, 35, 1405, 1734, 2196 |
| AcuI | CTGAAG | 2 cut(s) | 191, 464 |
| AcyI | GRCGYC | 1 cut(s) | 63 |
| AfaI | GTAC | 6 cut(s) | 402, 418, 1007, 1781, 1821, 2140 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 335, 356, 1305 |
| AgeI | ACCGGT | 1 cut(s) | 322 |
| AgsI | TTSAA | 7 cut(s) | 643, 1153, 1215, 1232, 1769, 1919, 2350 |
| AhlI | ACTAGT | 1 cut(s) | 421 |
| AjnI | CCWGG | 5 cut(s) | 1257, 1298, 1374, 1591, 2274 |
| Alw21I | GWGCWC | 2 cut(s) | 228, 2313 |
| Alw26I | GTCTC | 1 cut(s) | 1094 |
| Ama87I | CYCGRG | 1 cut(s) | 1446 |
| AoxI | GGCC | 5 cut(s) | 138, 156, 263, 843, 2106 |
| ApeKI | GCWGC | 9 cut(s) | 117, 149, 498, 541, 878, 988, 1015, 1412, 1694 |
| ApoI | RAATTY | 5 cut(s) | 23, 35, 1405, 1734, 2196 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1977, 2009 |
| AsiGI | ACCGGT | 1 cut(s) | 322 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1236, 1749 |
| AspLEI | GCGC | 3 cut(s) | 65, 294, 825 |
| AsuC2I | CCSGG | 5 cut(s) | 43, 531, 603, 1629, 1755 |
| AsuHPI | GGTGA | 5 cut(s) | 80, 570, 1170, 1380, 1463 |
| AsuNHI | GCTAGC | 1 cut(s) | 1397 |
| AvaI | CYCGRG | 1 cut(s) | 1446 |
| AxyI | CCTNAGG | 2 cut(s) | 1548, 2333 |
| BaeGI | GKGCMC | 1 cut(s) | 259 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 408, 441, 2292, 2325 |
| BalI | TGGCCA | 1 cut(s) | 2108 |
| BanI | GGYRCC | 1 cut(s) | 62 |
| BanII | GRGCYC | 1 cut(s) | 228 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 777, 809, 2108, 2140 |
| BbsI | GAAGAC | 1 cut(s) | 1668 |
| Bbv12I | GWGCWC | 2 cut(s) | 228, 2313 |
| BbvCI | CCTCAGC | 1 cut(s) | 1242 |
| BbvI | GCAGC | 9 cut(s) | 129, 136, 510, 553, 865, 975, 1027, 1424, 1681 |
| BccI | CCATC | 5 cut(s) | 418, 589, 1189, 1334, 1829 |
| BceAI | ACGGC | 1 cut(s) | 125 |
| BciT130I | CCWGG | 5 cut(s) | 1259, 1300, 1376, 1593, 2276 |
| BclI | TGATCA | 3 cut(s) | 850, 1324, 1537 |
| BcnI | CCSGG | 5 cut(s) | 43, 531, 603, 1629, 1755 |
| BcoDI | GTCTC | 1 cut(s) | 1094 |
| BcuI | ACTAGT | 1 cut(s) | 421 |
| BfaI | CTAG | 4 cut(s) | 422, 687, 1398, 2295 |
| BfmI | CTRYAG | 3 cut(s) | 1410, 1695, 2214 |
| BfoI | RGCGCY | 1 cut(s) | 66 |
| BfuAI | ACCTGC | 1 cut(s) | 2104 |
| BmcAI | AGTACT | 1 cut(s) | 1007 |
| BmeT110I | CYCGRG | 1 cut(s) | 1446 |
| BmiI | GGNNCC | 8 cut(s) | 64, 327, 348, 1239, 1365, 1633, 2094, 2145 |
| BmrI | ACTGGG | 1 cut(s) | 2142 |
| BmsI | GCATC | 9 cut(s) | 301, 448, 654, 865, 890, 940, 1021, 1444, 2260 |
| BmtI | GCTAGC | 1 cut(s) | 1401 |
| BmuI | ACTGGG | 1 cut(s) | 2142 |
| BpiI | GAAGAC | 1 cut(s) | 1668 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 23, 55 |
| BpmI | CTGGAG | 1 cut(s) | 836 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1242 |
| BpuEI | CTTGAG | 2 cut(s) | 242, 478 |
| BpuMI | CCSGG | 5 cut(s) | 43, 531, 603, 1629, 1755 |
| BsaBI | GATNNNNATC | 4 cut(s) | 315, 1308, 1542, 1704 |
| BsaHI | GRCGYC | 1 cut(s) | 63 |
| BsaWI | WCCGGW | 2 cut(s) | 322, 343 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1616, 1646, 1944, 1974 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 335, 356, 1305 |
| Bse118I | RCCGGY | 2 cut(s) | 65, 322 |
| Bse1I | ACTGG | 5 cut(s) | 1239, 1658, 1800, 2148, 2236 |
| Bse21I | CCTNAGG | 2 cut(s) | 1548, 2333 |
| Bse3DI | GCAATG | 3 cut(s) | 805, 825, 1057 |
| Bse8I | GATNNNNATC | 4 cut(s) | 315, 1308, 1542, 1704 |
| BseBI | CCWGG | 5 cut(s) | 1259, 1300, 1376, 1593, 2276 |
| BseGI | GGATG | 6 cut(s) | 186, 581, 590, 955, 980, 1345 |
| BseJI | GATNNNNATC | 4 cut(s) | 315, 1308, 1542, 1704 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 335, 356, 1305 |
| BseMI | GCAATG | 3 cut(s) | 805, 825, 1057 |
| BseMII | CTCAG | 8 cut(s) | 21, 192, 279, 1089, 1158, 1256, 1539, 1680 |
| BseNI | ACTGG | 5 cut(s) | 1239, 1658, 1800, 2148, 2236 |
| BseRI | GAGGAG | 3 cut(s) | 453, 481, 1624 |
| BseSI | GKGCMC | 1 cut(s) | 259 |
| BseXI | GCAGC | 9 cut(s) | 129, 136, 510, 553, 865, 975, 1027, 1424, 1681 |
| Bsh1236I | CGCG | 2 cut(s) | 268, 294 |
| Bsh1285I | CGRYCG | 1 cut(s) | 19 |
| BshFI | GGCC | 5 cut(s) | 140, 158, 265, 845, 2108 |
| BshNI | GGYRCC | 1 cut(s) | 62 |
| BshTI | ACCGGT | 1 cut(s) | 322 |
| BsiEI | CGRYCG | 1 cut(s) | 19 |
| BsiHKAI | GWGCWC | 2 cut(s) | 228, 2313 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1446 |
| BsiSI | CCGG | 9 cut(s) | 42, 57, 66, 323, 344, 530, 603, 1629, 1754 |
| BsiWI | CGTACG | 1 cut(s) | 2138 |
| BslFI | GGGAC | 1 cut(s) | 1807 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 335, 356, 1305 |
| BsmAI | GTCTC | 1 cut(s) | 1094 |
| BsmFI | GGGAC | 1 cut(s) | 1807 |
| BsnI | GGCC | 5 cut(s) | 140, 158, 265, 845, 2108 |
| BsoBI | CYCGRG | 1 cut(s) | 1446 |
| Bsp1286I | GDGCHC | 3 cut(s) | 228, 259, 2313 |
| BspACI | CCGC | 7 cut(s) | 60, 152, 266, 386, 479, 548, 1776 |
| BspANI | GGCC | 5 cut(s) | 140, 158, 265, 845, 2108 |
| BspCNI | CTCAG | 8 cut(s) | 22, 193, 280, 1090, 1157, 1255, 1540, 1681 |
| BspFNI | CGCG | 2 cut(s) | 268, 294 |
| BspHI | TCATGA | 1 cut(s) | 724 |
| BspLI | GGNNCC | 8 cut(s) | 64, 327, 348, 1239, 1365, 1633, 2094, 2145 |
| BspMAI | CTGCAG | 2 cut(s) | 1414, 1699 |
| BspMI | ACCTGC | 1 cut(s) | 2104 |
| BspOI | GCTAGC | 1 cut(s) | 1401 |
| BspT107I | GGYRCC | 1 cut(s) | 62 |
| BsrDI | GCAATG | 3 cut(s) | 805, 825, 1057 |
| BsrFI | RCCGGY | 2 cut(s) | 65, 322 |
| BsrI | ACTGG | 5 cut(s) | 1239, 1658, 1800, 2148, 2236 |
| BssAI | RCCGGY | 2 cut(s) | 65, 322 |
| BssNI | GRCGYC | 1 cut(s) | 63 |
| BssT1I | CCWWGG | 5 cut(s) | 281, 298, 449, 635, 1136 |
| Bst2UI | CCWGG | 5 cut(s) | 1259, 1300, 1376, 1593, 2276 |
| Bst4CI | ACNGT | 4 cut(s) | 11, 1674, 2123, 2129 |
| Bst6I | CTCTTC | 2 cut(s) | 174, 732 |
| BstACI | GRCGYC | 1 cut(s) | 63 |
| BstC8I | GCNNGC | 4 cut(s) | 48, 546, 559, 1399 |
| BstDEI | CTNAG | 9 cut(s) | 30, 201, 288, 1098, 1144, 1242, 1548, 1689, 2333 |
| BstDSI | CCRYGG | 1 cut(s) | 141 |
| BstF5I | GGATG | 6 cut(s) | 186, 581, 590, 955, 980, 1345 |
| BstFNI | CGCG | 2 cut(s) | 268, 294 |
| BstH2I | RGCGCY | 1 cut(s) | 66 |
| BstHHI | GCGC | 3 cut(s) | 65, 294, 825 |
| BstMAI | GTCTC | 1 cut(s) | 1094 |
| BstMCI | CGRYCG | 1 cut(s) | 19 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 146, 155, 554, 887, 1084, 2105 |
| BstNI | CCWGG | 5 cut(s) | 1259, 1300, 1376, 1593, 2276 |
| BstSFI | CTRYAG | 3 cut(s) | 1410, 1695, 2214 |
| BstSLI | GKGCMC | 1 cut(s) | 259 |
| BstUI | CGCG | 2 cut(s) | 268, 294 |
| BstV1I | GCAGC | 9 cut(s) | 129, 136, 510, 553, 865, 975, 1027, 1424, 1681 |
| BstV2I | GAAGAC | 1 cut(s) | 1668 |
| BstX2I | RGATCY | 5 cut(s) | 302, 706, 865, 1309, 1543 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1375 |
| BstYI | RGATCY | 5 cut(s) | 302, 706, 865, 1309, 1543 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1548, 2333 |
| BsuRI | GGCC | 5 cut(s) | 140, 158, 265, 845, 2108 |
| BtgI | CCRYGG | 1 cut(s) | 141 |
| BtsCI | GGATG | 6 cut(s) | 186, 581, 590, 955, 980, 1345 |
| BtsIMutI | CAGTG | 1 cut(s) | 2155 |
| BveI | ACCTGC | 1 cut(s) | 2104 |
| Cac8I | GCNNGC | 4 cut(s) | 48, 546, 559, 1399 |
| CciI | TCATGA | 1 cut(s) | 724 |
| CfoI | GCGC | 3 cut(s) | 65, 294, 825 |
| Cfr10I | RCCGGY | 2 cut(s) | 65, 322 |
| CseI | GACGC | 2 cut(s) | 283, 827 |
| Csp6I | GTAC | 6 cut(s) | 401, 417, 1006, 1780, 1820, 2139 |
| CspAI | ACCGGT | 1 cut(s) | 322 |
| CviAII | CATG | 4 cut(s) | 725, 731, 745, 1171 |
| CviQI | GTAC | 6 cut(s) | 401, 417, 1006, 1780, 1820, 2139 |
| DdeI | CTNAG | 9 cut(s) | 30, 201, 288, 1098, 1144, 1242, 1548, 1689, 2333 |
| DinI | GGCGCC | 1 cut(s) | 64 |
| DraI | TTTAAA | 2 cut(s) | 1069, 2056 |
| EaeI | YGGCCR | 1 cut(s) | 2106 |
| Eam1104I | CTCTTC | 2 cut(s) | 174, 732 |
| EarI | CTCTTC | 2 cut(s) | 174, 732 |
| Ecl136II | GAGCTC | 1 cut(s) | 226 |
| Eco130I | CCWWGG | 5 cut(s) | 281, 298, 449, 635, 1136 |
| Eco24I | GRGCYC | 1 cut(s) | 228 |
| Eco53kI | GAGCTC | 1 cut(s) | 226 |
| Eco57I | CTGAAG | 2 cut(s) | 191, 464 |
| Eco81I | CCTNAGG | 2 cut(s) | 1548, 2333 |
| Eco88I | CYCGRG | 1 cut(s) | 1446 |
| EcoICRI | GAGCTC | 1 cut(s) | 226 |
| EcoRI | GAATTC | 2 cut(s) | 1405, 2196 |
| EcoRII | CCWGG | 5 cut(s) | 1257, 1298, 1374, 1591, 2274 |
| EcoT14I | CCWWGG | 5 cut(s) | 281, 298, 449, 635, 1136 |
| EcoT22I | ATGCAT | 1 cut(s) | 943 |
| EcoT38I | GRGCYC | 1 cut(s) | 228 |
| EgeI | GGCGCC | 1 cut(s) | 64 |
| EheI | GGCGCC | 1 cut(s) | 64 |
| ErhI | CCWWGG | 5 cut(s) | 281, 298, 449, 635, 1136 |
| FaeI | CATG | 4 cut(s) | 728, 734, 748, 1174 |
| FaqI | GGGAC | 1 cut(s) | 1807 |
| FatI | CATG | 4 cut(s) | 724, 730, 744, 1170 |
| FauI | CCCGC | 1 cut(s) | 1769 |
| FauNDI | CATATG | 2 cut(s) | 937, 1827 |
| FbaI | TGATCA | 3 cut(s) | 850, 1324, 1537 |
| FokI | GGATG | 6 cut(s) | 173, 568, 577, 962, 967, 1352 |
| FriOI | GRGCYC | 1 cut(s) | 228 |
| FspBI | CTAG | 4 cut(s) | 422, 687, 1398, 2295 |
| GlaI | GCGC | 3 cut(s) | 64, 293, 824 |
| GsuI | CTGGAG | 1 cut(s) | 836 |
| HaeII | RGCGCY | 1 cut(s) | 66 |
| HaeIII | GGCC | 5 cut(s) | 140, 158, 265, 845, 2108 |
| HapII | CCGG | 9 cut(s) | 42, 57, 66, 323, 344, 530, 603, 1629, 1754 |
| HgaI | GACGC | 2 cut(s) | 283, 827 |
| HhaI | GCGC | 3 cut(s) | 65, 294, 825 |
| Hin1I | GRCGYC | 1 cut(s) | 63 |
| Hin1II | CATG | 4 cut(s) | 728, 734, 748, 1174 |
| Hin6I | GCGC | 3 cut(s) | 63, 292, 823 |
| HinP1I | GCGC | 3 cut(s) | 63, 292, 823 |
| HindIII | AAGCTT | 2 cut(s) | 888, 1746 |
| HinfI | GANTC | 7 cut(s) | 217, 248, 439, 721, 1115, 1588, 1942 |
| HpaII | CCGG | 9 cut(s) | 42, 57, 66, 323, 344, 530, 603, 1629, 1754 |
| HphI | GGTGA | 5 cut(s) | 80, 570, 1170, 1380, 1463 |
| Hpy166II | GTNNAC | 1 cut(s) | 419 |
| Hpy8I | GTNNAC | 1 cut(s) | 419 |
| HpyAV | CCTTC | 7 cut(s) | 130, 514, 600, 604, 1100, 2110, 2370 |
| HpyCH4III | ACNGT | 4 cut(s) | 11, 1674, 2123, 2129 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 146, 155, 554, 887, 1084, 2105 |
| HpyF3I | CTNAG | 9 cut(s) | 30, 201, 288, 1098, 1144, 1242, 1548, 1689, 2333 |
| Hsp92I | GRCGYC | 1 cut(s) | 63 |
| Hsp92II | CATG | 4 cut(s) | 728, 734, 748, 1174 |
| HspAI | GCGC | 3 cut(s) | 63, 292, 823 |
| KasI | GGCGCC | 1 cut(s) | 62 |
| Ksp22I | TGATCA | 3 cut(s) | 850, 1324, 1537 |
| LmnI | GCTCC | 5 cut(s) | 86, 466, 1092, 1637, 1859 |
| Lsp1109I | GCAGC | 9 cut(s) | 129, 136, 510, 553, 865, 975, 1027, 1424, 1681 |
| LweI | GCATC | 9 cut(s) | 301, 448, 654, 865, 890, 940, 1021, 1444, 2260 |
| MaeI | CTAG | 4 cut(s) | 422, 687, 1398, 2295 |
| MaeIII | GTNAC | 6 cut(s) | 11, 1116, 1176, 1441, 1498, 2123 |
| MfeI | CAATTG | 2 cut(s) | 1041, 1582 |
| MflI | RGATCY | 5 cut(s) | 302, 706, 865, 1309, 1543 |
| MhlI | GDGCHC | 3 cut(s) | 228, 259, 2313 |
| MlsI | TGGCCA | 1 cut(s) | 2108 |
| MluNI | TGGCCA | 1 cut(s) | 2108 |
| Mly113I | GGCGCC | 1 cut(s) | 63 |
| MlyI | GAGTC | 3 cut(s) | 448, 1124, 1597 |
| MmeI | TCCRAC | 2 cut(s) | 2173, 2204 |
| Mox20I | TGGCCA | 1 cut(s) | 2108 |
| Mph1103I | ATGCAT | 1 cut(s) | 943 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1236, 1749 |
| MscI | TGGCCA | 1 cut(s) | 2108 |
| MslI | CAYNNNNRTG | 3 cut(s) | 729, 1169, 1437 |
| Msp20I | TGGCCA | 1 cut(s) | 2108 |
| MspI | CCGG | 9 cut(s) | 42, 57, 66, 323, 344, 530, 603, 1629, 1754 |
| MunI | CAATTG | 2 cut(s) | 1041, 1582 |
| MvaI | CCWGG | 5 cut(s) | 1259, 1300, 1376, 1593, 2276 |
| MvnI | CGCG | 2 cut(s) | 268, 294 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 146, 155, 554, 887, 1084, 2105 |
| NarI | GGCGCC | 1 cut(s) | 63 |
| NciI | CCSGG | 5 cut(s) | 43, 531, 603, 1629, 1755 |
| NdeI | CATATG | 2 cut(s) | 937, 1827 |
| NheI | GCTAGC | 1 cut(s) | 1397 |
| NlaIII | CATG | 4 cut(s) | 728, 734, 748, 1174 |
| NlaIV | GGNNCC | 8 cut(s) | 64, 327, 348, 1239, 1365, 1633, 2094, 2145 |
| NmuCI | GTSAC | 3 cut(s) | 1116, 1176, 1498 |
| NsiI | ATGCAT | 1 cut(s) | 943 |
| PaeR7I | CTCGAG | 1 cut(s) | 1446 |
| PagI | TCATGA | 1 cut(s) | 724 |
| PasI | CCCWGGG | 1 cut(s) | 1258 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1236, 1749 |
| PfeI | GAWTC | 4 cut(s) | 217, 248, 721, 1942 |
| Pfl23II | CGTACG | 1 cut(s) | 2138 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1305 |
| PfoI | TCCNGGA | 2 cut(s) | 601, 1591 |
| PinAI | ACCGGT | 1 cut(s) | 322 |
| Ple19I | CGATCG | 1 cut(s) | 19 |
| PleI | GAGTC | 3 cut(s) | 447, 1123, 1596 |
| PluTI | GGCGCC | 1 cut(s) | 66 |
| PpsI | GAGTC | 3 cut(s) | 447, 1123, 1596 |
| Psp124BI | GAGCTC | 1 cut(s) | 228 |
| Psp6I | CCWGG | 5 cut(s) | 1257, 1298, 1374, 1591, 2274 |
| PspGI | CCWGG | 5 cut(s) | 1257, 1298, 1374, 1591, 2274 |
| PspLI | CGTACG | 1 cut(s) | 2138 |
| PspN4I | GGNNCC | 8 cut(s) | 64, 327, 348, 1239, 1365, 1633, 2094, 2145 |
| PspXI | VCTCGAGB | 1 cut(s) | 1446 |
| PstI | CTGCAG | 2 cut(s) | 1414, 1699 |
| PsuI | RGATCY | 5 cut(s) | 302, 706, 865, 1309, 1543 |
| PvuI | CGATCG | 1 cut(s) | 19 |
| RsaI | GTAC | 6 cut(s) | 402, 418, 1007, 1781, 1821, 2140 |
| RsaNI | GTAC | 6 cut(s) | 401, 417, 1006, 1780, 1820, 2139 |
| RseI | CAYNNNNRTG | 3 cut(s) | 729, 1169, 1437 |
| SacI | GAGCTC | 1 cut(s) | 228 |
| ScaI | AGTACT | 1 cut(s) | 1007 |
| SchI | GAGTC | 3 cut(s) | 448, 1124, 1597 |
| SduI | GDGCHC | 3 cut(s) | 228, 259, 2313 |
| SfaNI | GCATC | 9 cut(s) | 301, 448, 654, 865, 890, 940, 1021, 1444, 2260 |
| SfcI | CTRYAG | 3 cut(s) | 1410, 1695, 2214 |
| SfoI | GGCGCC | 1 cut(s) | 64 |
| Sfr274I | CTCGAG | 1 cut(s) | 1446 |
| SgrAI | CRCCGGYG | 1 cut(s) | 65 |
| SlaI | CTCGAG | 1 cut(s) | 1446 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 729, 1169, 1437 |
| SmlI | CTYRAG | 3 cut(s) | 221, 493, 1446 |
| SmoI | CTYRAG | 3 cut(s) | 221, 493, 1446 |
| SpeI | ACTAGT | 1 cut(s) | 421 |
| SsiI | CCGC | 7 cut(s) | 60, 152, 266, 386, 479, 548, 1776 |
| SspDI | GGCGCC | 1 cut(s) | 62 |
| SspMI | CTAG | 4 cut(s) | 422, 687, 1398, 2295 |
| SstI | GAGCTC | 1 cut(s) | 228 |
| StyI | CCWWGG | 5 cut(s) | 281, 298, 449, 635, 1136 |
| TaaI | ACNGT | 4 cut(s) | 11, 1674, 2123, 2129 |
| TaqI | TCGA | 5 cut(s) | 228, 968, 1447, 1722, 1854 |
| TatI | WGTACW | 2 cut(s) | 400, 1005 |
| TauI | GCSGC | 2 cut(s) | 63, 268 |
| TfiI | GAWTC | 4 cut(s) | 217, 248, 721, 1942 |
| TscAI | CASTG | 1 cut(s) | 2155 |
| TseFI | GTSAC | 3 cut(s) | 1116, 1176, 1498 |
| TseI | GCWGC | 9 cut(s) | 117, 149, 498, 541, 878, 988, 1015, 1412, 1694 |
| Tsp45I | GTSAC | 3 cut(s) | 1116, 1176, 1498 |
| TspGWI | ACGGA | 3 cut(s) | 766, 769, 2156 |
| TspRI | CASTG | 1 cut(s) | 2155 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1305 |
| XapI | RAATTY | 5 cut(s) | 23, 35, 1405, 1734, 2196 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1832 |
| XhoI | CTCGAG | 1 cut(s) | 1446 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1236, 1749 |
| XspI | CTAG | 4 cut(s) | 422, 687, 1398, 2295 |
| ZrmI | AGTACT | 1 cut(s) | 1007 |
| Zsp2I | ATGCAT | 1 cut(s) | 943 |